• To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR/FRAP), represents one of these pathways, we have examined the effect of simultaneous inhibition of the Ras-MAPK and PI3K-mTOR pathways on transformation of CEF by v-Src. (embl.de)
  • Background: PIP 2 generated by PIPKI family members regulates many cellular functions, and PIP 2 is a PI3K substrate. (nebraska.edu)
  • Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). (nih.gov)
  • Phosphoinositide-3-kinase (PI3K) that phosphorylates PftdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). (scbdd.com)
  • a phosphatidylinositol 3-kinase (PI3K) to phosphorylate phosphatidylinositol 4,5-bisphosphate and produce phosphatidylinositol 3,4,5-trisphosphate, a major growth signal for activation of Akt kinase. (molcells.org)
  • This phosphorylation generates phosphatidylinositol 4-phosphate (PI4P), a docking site normally located at the Golgi membrane. (wikipedia.org)
  • Serving a critical role in cell signaling, the class I family of phosphoinositide 3-kinases (PI3Ks) catalyze the phosphorylation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ] to generate PI(3,4,5) P 3 . (elifesciences.org)
  • Inositol polyphosphate multikinase (IPMK) is required for the biosynthesis of inositol phosphates (IPs) through the phosphorylation of multiple IP metabolites such as IP3 and IP4. (molcells.org)
  • Common themes to emerge from studies of picornavirus replication are the need for cholesterol and phosphatidylinositol 4-phosphate (PI4P). (microbiologyresearch.org)
  • In infected cells, type III phosphatidylinositol 4-kinases (PI4KIIIs) generate elevated levels of PI4P, which is then exchanged for cholesterol at replication organelles. (microbiologyresearch.org)
  • Other picornaviruses, for example the cardioviruses, are believed to initiate replication at the endoplasmic reticulum and subvert PI4KIIIα to generate PI4P. (microbiologyresearch.org)
  • Five candidate genes for pollen-expressed Arabidopsis phosphatidylinositol-4-phosphate 5-kinases (PI4P 5-kinases) were identified and the recombinant proteins characterized regarding catalytic activity and substrate preferences. (uni-goettingen.de)
  • The data indicate that PI4P 5-kinase isoforms generate distinct PI(4,5)P2-pools that control either actin-dynamics or exocytosis of cell wall-material, respectively, and that these pools coexist within close proximity of the apical plasma membrane of pollen tubes. (uni-goettingen.de)
  • Daher wurden an Hand von Sequenzähnlichkeiten und publizierten Genexpressionsdaten fünf Kandidaten-gene identifiziert, welche möglicherweise Phosphatidylinositol-4-phosphat 5-Kinasen (PI4P 5-Kinasen) kodieren und in Pollenschläuchen exprimiert werden. (uni-goettingen.de)
  • Second, choline-phosphate cytidylyltransferase, localized to the endoplasmic reticulum membrane, catalyzes the conversion of phosphocholine to CDP-choline. (smpdb.ca)
  • Orthologous to human PIK3C2A (phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha). (nih.gov)
  • Enables 1-phosphatidylinositol-3-kinase activity. (nih.gov)
  • Predicted to be part of phosphatidylinositol 3-kinase complex. (nih.gov)
  • PI4KA is a kinase, which phosphorylates a hydroxyl group of phosphatidylinositol in its inositol-ring at position four. (wikipedia.org)
  • Phosphatidylinositol-4 kinase III beta and oxysterol-binding protein accumulate unesterified cholesterol on poliovirus-induced membrane structure. (microbiologyresearch.org)
  • We report here that Sac1p has a specific role in secretion and acts as an antagonist of the phosphatidylinositol 4-kinase Pik1p in Golgi trafficking. (embl.de)
  • Synthesis of new cyclitol compounds that influence the activity of phosphatidylinositol 4-kinase isoform, PI4K230. (embl.de)
  • The synthesis, chemical derivatization, and investigation of the inhibitory properties of novel cyclitol derivatives on the phosphatidylinositol 4-kinase enzymes PI4K55 and PI4K230 involved in the phosphatidylinositol cycle are reported. (embl.de)
  • The phosphatidylinositol 3-phosphate (PtdIns3P) - generating kinase complex is not restricted to selective autophagy, but is also involved in general autophagy, vesicle trafficking, cytokinesis and endocytosis. (ruhr-uni-bochum.de)
  • A previous screen of a human kinase and phosphatase shRNA library to select genes that mediate arsenite induction of spindle abnormalities resulted in the identification of phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PIP4KIIγ), a phosphatidylinositol 4,5-bisphosphate (PIP2)-synthesizing enzyme. (biomedcentral.com)
  • Monomeric GTPases of the Rho and ARF families which are engaged downstream of activated receptors, in cooperation with phosphatidylinositol 4-phosphate 5-kinase and phosphatidylinositol 3-kinase lipid modifying enzymes, can modulate locally the assembly of the submembranous actin filament system leading to particle internalization [ 3345 , 5261 ]. (nanomedicine.com)
  • The main question in both cases concerns the functional role of the posttranslational modification of proteins with ubiquitin, as well as the impact of signalling lipids like phosphatidylinositol 3-phosphate (PtdIns3P). (ruhr-uni-bochum.de)
  • Critical for cellular organization, phosphatidylinositol phosphate (PIP) lipids regulate the localization and activity of numerous proteins across intracellular membranes in eukaryotic cells ( Di Paolo and De Camilli 2006 ). (elifesciences.org)
  • Mass spectrometric analysis of lipids from slc1Δ and slc4Δ cells demonstrates that in vivo Slc1p and Slc4p generate almost the same glycerophospholipid profile. (unifr.ch)
  • The recruitment of specific cytosolic proteins to intracellular membranes through binding phosphorylated derivatives of phosphatidylinositol (PtdIns) controls such processes as endocytosis, regulated exocytosis, cytoskeletal organization, and cell signaling. (embl.de)
  • Protein modules such as FVYE domains and PH domains that bind specifically to PtdIns 3-phosphate (PtdIns-3-P) and polyphosphoinositides, respectively, can direct such membrane targeting. (embl.de)
  • Indeed, affinity-purified Slc1p displays Mg²⁺-dependent acyltransferase activity not only towards lyso-phosphatidic acid but also lyso forms of phosphatidylserine and phosphatidylinositol. (unifr.ch)
  • The DHPC-binding mode of the cPLA 2 α C2-domain, which differs from phosphatidylserine or phosphatidylinositol 4,5-bisphosphate binding by other C2-domains, expands and deepens knowledge of the lipid-binding mechanisms mediated by C2-domains. (rcsb.org)
  • Therefore, we also elucidate the requirements that define where and when PtdIns3P is generated and how the outcome of the subsequent signalling cascade is integrated in one of the mentioned cellular events. (ruhr-uni-bochum.de)
  • These enzymes hydrolyze sphingomyelin and glycerophospholipids, respectively, generating products identical to the ones produced by eukaryotic enzymes which play crucial roles in distinct physiological processes, including membrane dynamics, cellular signaling, migration, growth, and death. (inra.fr)
  • These viruses use PI4KIIIa to generate PI(4)P-enriched environments increasing cellular levels of PI(4)P. PI4KIIIb (PI4KB), regulates the golgi reorganization during mitosis. (echelon-inc.com)
  • Phosphatidylinositol 4-phosphate (PI(4)P) is the most abundant monophosphorylated phosphoinositide found in mammalian cells. (echelon-inc.com)
  • Design of drug-resistant alleles of type-III phosphatidylinositol 4-kinases using mutagenesis and molecular modeling. (microbiologyresearch.org)
  • We have found that Arf6 acts through activation of type I phosphatidylinositol 4-phosphate 5-kinases to generate phosphatidylinositol 4,5-bisphosphate and that this activity is instrumental in facilitating the actin cytoskeletal rearrangements and alterations in endosomal membrane trafficking observed with increased Arf6 activation. (portlandpress.com)
  • Arf1 can also stimulate the activity of phosphatidylinositol kinases and recruit coat proteins and actin cytoskeletal elements to the Golgi complex. (portlandpress.com)
  • IP6 can be further metabolized by the inositol-hexakisphosphate kinases (IP6Ks), which add a phosphate at the 5-position to produce 5-diphosphoinositol pentakisphosphate (5-PP-IP5,5-IP7). (molcells.org)
  • To address this question, we generated Vps34 knock-out mouse embryonic fibroblasts (MEFs) and analyzed the impact of Vps34 deletion on autophagy in mammalian cells. (columbia.edu)
  • Lipidomics is an emerging powerful tool with the potential to identify new interconnected metabolic lipid networks as well as generate new hypotheses. (columbia.edu)
  • However, the lipid profiles generated by microsomes from slc1Δ and slc4Δ cells are different and this suggests that Slc1p and Slc4p have a different substrate specificity or have access to different lyso-glycerophospholipid substrates because of a different subcellular location. (unifr.ch)
  • It is also a novel regulator of tumor growth and generates PtdIns4P-rich domains within the Golgi. (echelon-inc.com)
  • DFCP1 generates cup-shaped protrusions from the ER membrane, termed omegasomes, which provide a platform for the production of autophagosomes. (whiterose.ac.uk)
  • This observation is reminiscent of the transient association of LC3 and DFCP1 during omegasome formation, and therefore we propose that omegasomes are utilized by HCV to generate the double-membrane vesicles which are the hallmark of HCV replication complexes. (whiterose.ac.uk)
  • We show that the DOCK homology region (DHR)-1 domain of DOCK8 binds specifically to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and is required for its recruitment to the plasma membrane. (life-science-alliance.org)
  • Phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) occurs in the tip-plasma membrane of growing pollen tubes. (uni-goettingen.de)
  • SLC4 harbors a membrane bound O-acyl transferase (MBOAT) motif, and down regulation of SLC4 strongly reduces 1-acyl-sn-glycerol-3-phosphate acyltransferase activity in microsomes from slc1Δ cells. (unifr.ch)
  • PLCs are activated by external stimuli such as growth factors to cleave phosphatidylinositol 4,5-bisphosphate and produce IP3, which is then released from the cell membrane into the cytosol. (molcells.org)
  • Therefore, the aims of the present study were to determine systematically the effects of a clinically relevant concentration of rapamycin on cytokine production, T cell stimulatory capacity and CD8+ Treg-generating capacity of human PDC. (c-kitinhibitors.com)
  • Phosphatidylinositol 4,5-bisphosphate (PI4,5P2, hereafter referred to as PIP2) either binds to intracellular proteins and directly modulates their subcellular localization and activity or acts as a precursor for the generation of other second messengers. (biomedcentral.com)
  • Deletion of SLC1 is not lethal and does not eliminate all microsomal 1-acylglycerol-3-phosphate O-acyltransferase activity suggesting that an additional enzyme may exist. (unifr.ch)
  • Gelsolin regulates the dynamic rearrangement of the actin cytoskeleton by severing actin filaments (F-actin) and capping the newly generated barbed filament ends. (ubc.ca)
  • Description: RNA profiling data sets generated by the Mouse ENCODE project. (nih.gov)
  • Thus, Slc1p and Slc4p may not only be active as 1-acylglycerol-3-phosphate O-acyltransferases, but also be involved in fatty acid exchange at the sn-2 position of mature glycerophospholipids. (unifr.ch)
  • The 7-fold repeat domain is homologous to a similar domain in phosphatidylinositol-glycan-specific phospholipase D. (embl.de)
  • One of these Ca 2+ ions, along with a third Ca 2+ , bridges the C2-domain to the DHPC phosphate group, which also interacts with Asn65. (rcsb.org)
  • Here we show that SLC4 (Yor175c), a gene of hitherto unknown function, encodes a second 1-acyl-sn-glycerol-3-phosphate acyltransferase. (unifr.ch)
  • Polycarbonate plastics may contain bisphenol A, and the effects of exposure to these 3D printer-generated emissions on neuroendocrine function are similar to those noted following exposure to bisphenol A. (cdc.gov)
  • Receptors able to mediate phagocytosis are expressed almost exclusively in neutrophils, monocytes and macrophages, and receptor clustering is thought to occur upon particle binding which in turn generates a phagocytic signal, activating the phagocytic process [ 3345 ]. (nanomedicine.com)
  • During an infection, chemotactic agents are generated that attract neutrophils to the site of infection, which in turn play a critical role in phagocytosing and killing microorganisms. (medscape.com)
  • The increased use of this technology has resulted in questions regarding the influence of inhaling emissions generated during printing. (cdc.gov)
  • Previous study revealed that the protective effect of TIGAR in cell survival is mediated through the increase in PPP (pentose phosphate pathway) flux. (nature.com)
  • The goal of this study was to determine if inhalation of particulate and/or toxic chemicals generated during printing with polycarbonate (PC) plastic affected the neuroendocrine system. (cdc.gov)