Gene using luciferase reporter gene. Medical search. Frequent questions

Q1：Optimum reaction temperature for dual luciferase reporter gene? (yeasenbiotech.com)
Q2：How to choose a dual luciferase reporter gene vector? (yeasenbiotech.com)
The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
Dual-luciferase reporter gene assays demonstrated Arhgap11a as the target of miR-211-5p. (frontiersin.org)

Firefly luciferase and Renilla luciferase detection systems are mostly used in traditional reporter gene detection systems, but the short half-life of the luminescent signal and poor stability, etc., affect the experimental results and use and cause inconvenience. (yeasenbiotech.com)
During the actual experiment, due to the inconsistent quality of the plasmids extracted from firefly luciferase and Renilla luciferase, and the different regulatory capabilities of the target regulatory elements to be detected, we recommend pre-experiment optimization of firefly luciferase. (yeasenbiotech.com)
Both Firefly luciferase and Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. (addgene.org)

Other luciferases in our collection include Gaussia luciferase (Gluc) and the red and green Click-beetle luciferases (CBRluc and CBGluc, respectively). (addgene.org)
Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short luminescence half-life makes it too dim for many experimental systems. (addgene.org)
A Gaussia luciferase reporter assay was used to determine if CORO1C is a putative YB-1 downstream target. (biomedcentral.com)
To facilitate development of antivirals against these viruses, we established two distinct screening platforms based on MARV and EBOV reverse genetics systems that express secreted Gaussia luciferase (gLuc). (genscript.com)

We examined the activities of cloned promoter DNA fragments with luciferase assay. (molvis.org)
We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
This method is an example of using cis-acting elements where the two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule. (wikipedia.org)
In addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive promoter and can be used to monitor transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of expression. (addgene.org)
Available in a variety of promoter-reporter combinations that enable multiple imaging modalities-fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)-you can select from lentivector or minicircle technologies for both integrated and episomal expression. (reportergene.com)
The CMV-Luciferase-EF1α-copGFP vector expresses luciferase from a CMV promoter for high expression in most cell types, and the bright copGFP from the EF1α promoter for medium expression. (reportergene.com)
GenTargetDescription:Premade lentivirus expressing human TERT gene under suCMV promoter, containing RFP-Puromycin marker.Storage conditions: (-80°C). (reportergene.com)
GenTargetDescription:Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing RFP-Puromycin marker. (reportergene.com)
To do this, first both the GBA gene mutations and the variations in the promoter of GlcCer synthase are analysed in patients with GD. (fundacionareces.es)
Luciferase reporter assays demonstrated that the Adcy1 promoter is selectively activated by neuronal PAS-domain protein 2 (NPAS2)/BMAL1. (jneurosci.org)
INDIGO's TR reporter cells include the luciferase reporter gene functionally linked to a responsive promoter. (thomassci.com)
The effect of the promoter variant was further studied by luciferase gene reporter assay in MCF-7, HEK293, HeLa and LNCaP cell lines. (biomedcentral.com)
Luciferase reporter gene assay of the promoter construct bearing this variant did not suggest a variation of expression in the MCF-7 breast cancer cell line, but indicated a reduction of luciferase expression in both the HEK293 and LNCaP cell lines. (biomedcentral.com)
The gfp gene expression is now under the control of the promoter for the β-tubulin gene. (jove.com)

Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
Examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed [fr]. (wikipedia.org)
A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. (wikipedia.org)
Reporter genes can produce a protein that has little obvious or immediate effect on the cell culture or organism. (wikipedia.org)
Reporter gene assay have been increasingly used in high throughput screening (HTS) to identify small molecule inhibitors and activators of protein targets and pathways for drug discovery and chemical biology. (wikipedia.org)
It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)
Activity of recombinant Lucia luciferase protein determined by measuring Relative Light Units (RLU) in a luminometer using QUANTI-Luc™ . (invivogen.com)
Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. (biomedcentral.com)
Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. (addgene.org)
NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available - these attributes have made it popular with scientists since its release. (addgene.org)
Silencing the YBX1 gene (which encodes the YB-1 protein) by small interfering RNA (siRNA) was performed in MDA-MB-231 and Hs578T breast cancer cell lines, followed by phenotypic assays including cell migration and invasion assays. (biomedcentral.com)
Global gene expression profiling in the YBX1 silenced MDA-MB-231 cells identified differential expression of several genes, including CORO1C (which encodes for an actin binding protein, coronin-1C) as a potential downstream target of YB-1. (biomedcentral.com)
Recent research has shed light on a potential biomarker for early metastasis, namely Y-box binding protein-1 (YB-1) encoded by the YBX1 gene. (biomedcentral.com)
The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
A reporter gene codes for a protein that can be tracked, such as a protein with a known enzymatic activity or one that is fluorescent. (jove.com)
One of the well-known reporter genes is one that encodes for a protein called green fluorescent protein - or GFP. (jove.com)
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. (jove.com)
Thus, reporter genes "report" the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell. (jove.com)
Commonly used reporter genes are - GFP (green fluorescent protein gene), lacZ (β- galactosidase gene), RFP (red fluorescent protein gene), and Luc (luciferase gene). (jove.com)

The payload is also DNA, which encodes both a reporter gene and the CRISPR/Cas9 system. (futurity.org)
mutations high frequency of IDH1/2 mutations in oligodendrogliomas, astrocytomas and in alteRations in the RB1 pathway in The TET2 gene encodes the -KG- secondary glioblastomas derived thereof low-gRade diffuse gliomas lacking dependent enzyme that catalyses suggests that these tumours share a common genetic alteRations the conversion of 5-methylcytosine to common progenitor cell population. (who.int)

Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
Furthermore, based on differentially expressed genes and WGCNA analyses, we found that the hypoxia signaling pathway was enriched under heat shock conditions. (biomedcentral.com)
Overexpression and silencing of YBX1 were performed to assess the expression of CORO1C , one of the differentially regulated genes from the transcriptomic analysis. (biomedcentral.com)
RNA sequencing identified differentially expressed genes (DEGs) post RBM39 knockdown. (bvsalud.org)

For technology of reporter plasmids, the 39 UTRs of human CASP3 and seven were amplified working with the oligonucleotides NotIhCASP3-3UTRf, XhoIhCASP3-3UTRr, NotIhCASP73UTRf and XhoIhCASP7-3UTRr (table S3). (signsin1dayinc.com)
Endotoxin-cost-free reporter plasmids (pTKGhCASP3 and pTKGhCASP7) were being developed for transfection employing NucleoBond Xtra Midi Furthermore EF (Macherey-Nagel GmbH & Co. KG). (signsin1dayinc.com)
The reporter plasmids pTKGhCASP3 or pTKGhCASP7 were cotransfected with Pre-miR miRNA Precursors hsa-enable-7e or hsamiR-29a (Lifetime Technologies), respectively. (signsin1dayinc.com)
Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression. (addgene.org)
Empty backbones for inserting regulatory elements before or after the luciferase ORF can be found in a table below, as well as plasmids that already contain a region of interest before or after luciferase . (addgene.org)
Welcome to Reportergene IMAGE clones, Plasmids & Lentivectors! (reportergene.com)

The Yeasen detection system interacts with luciferase, and the luminescence quenching effect of luciferin is comparable to that of the P* brand. (yeasenbiotech.com)
The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. (sigmaaldrich.com)
Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. (jove.com)

Co-transfection amount of plasmid and Renilla luciferase plasmid. (yeasenbiotech.com)
Nucleofection was carried out employing 561056106 HeLa making use of .9.eight mg reporter plasmid (pTK-Gluc derivatives, NEB GmbH), 100200 ng normalisation plasmid (pTK-Cluc, NEB GmbH) and MiRNA expression in contaminated human monocytes was learned using the miRCURY LNATM miRNA Array package v. ten. (signsin1dayinc.com)
Scientists have developed a wide range of luciferase-based plasmid systems for many different applications. (addgene.org)

Similar corresponding luciferase mRNA levels indicate translational rather than transcriptional alterations. (uzh.ch)
These data suggest that Sf accounts for most of the ARE-activated transcriptional induction of antioxidant genes by broccoli. (usda.gov)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)

In view of the shortcomings of traditional flash type luciferase, Sheng Bio has developed a new generation of glow type luciferase detection system with more convenient operation, longer half-life and better stability through painstaking research, suitable for samples such as mammalian cells, protoplasts, etc. (yeasenbiotech.com)
Other tumor imaging's techniques that rely upon the detection of gene expression (reporter) in the body of the animals have been developed [16] which made a significant impact on both the versatility and the specificity of tumor imaging of animals. (biotech-angels.com)
In addition to TR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. (thomassci.com)

Only those cells that have successfully taken up the construct containing the CAT gene will survive and multiply under these conditions. (wikipedia.org)
An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
Cells will be labeled with luciferase or other reporter genes. (stanford.edu)
thus its infectivity can be quantified by luciferase activity in virus-infected cells ( 5 - 7 ). (cdc.gov)
MDCK cells were infected with serially diluted pseudovirus stock, and the infectivity reflected by the relative luciferase activity (RLA) was determined as the median (50%) tissue culture infective dose, according to the method of Reed and Muench. (cdc.gov)
The CMV-Luciferase-EF1α-copGFP BLIV 2.0 Lentivector for In Vivo Imaging leverages SBI's well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. (reportergene.com)
Reporter gene expression was not affected upon exposure of transiently transfected cells to hypoxia (1% oxygen). (uzh.ch)
Our study demonstrates a circadian rhythm of contrast sensitivity that peaks during the daytime, and that its regulation involves interactions of D4Rs, the clock gene Npas2 , and the clock-controlled gene adenylyl cyclase 1 ( Adcy1 ) in a subset of retinal ganglion cells (RGCs). (jneurosci.org)
Gene expression profiling using Affymetrix GeneChip® Human Transcriptome 2.0 array was subsequently carried out in YB-1 silenced MDA-MB-231 cells. (biomedcentral.com)
While knockdown of YBX1 gene decreased CORO1C gene expression, the opposite effects were seen in YB-1 overexpressing cells. (biomedcentral.com)
Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. (nutrimedical.com)
INDIGO's panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. (thomassci.com)
Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in TRα or TRβ activity. (thomassci.com)
TR reporter cells are prepared using INDIGO's proprietary CryoMite™ process. (thomassci.com)
This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. (thomassci.com)
There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. (thomassci.com)
The pattern and timing of gene expression can be determined by creating recombinant DNA with a reporter gene under the control of a cis -regulatory sequence of interest and introducing it into cells or an organism. (jove.com)
Since the gene of interest and the reporter gene have the same cis -regulatory sequence, they are expressed in the same cells and at the same time. (jove.com)
The cells expressing the Luc gene produce luciferase enzymes that catalyze a reaction with luciferin to produce light. (jove.com)
In ER+ cancer cells, PIP5K1α acted on pSer-473 AKT, and was in complexes with VEGFR2, serving as co-factor of ER-alpha to regulate activities of target genes including cyclin D1 and CDK1. (lu.se)

Background Renilla Luciferase reporter gene (rLuc) GL4. (biotech-angels.com)
The most common types of luciferase found in Addgene's collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc), and NanoLuc® Luciferase (Nluc). (addgene.org)

In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. (wikipedia.org)
This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. (wikipedia.org)
An example of a selectable marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase (CAT) gene, which confers resistance to the antibiotic chloramphenicol. (wikipedia.org)
In the case of selectable-marker reporters such as CAT, the transfected population of bacteria can be grown on a substrate that contains chloramphenicol. (wikipedia.org)
Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. (addgene.org)

Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. (wikipedia.org)

Positive control for QUANTI-Luc™ , a luciferase reporter assay reagent. (invivogen.com)

The second platform is complementary to the first and expresses gLuc as a reporter gene product encoded in recombinant infect. (genscript.com)
The second platform is complementary to the first and expresses gLuc as a reporter gene product encoded in recombinant infectious MARV and EBOV, thereby allowing for rapid quantification of viral growth during treatment with antiviral compounds. (genscript.com)
This recombinant gene is introduced into different cell types, and both the gene of interest and the reporter gene are allowed to express. (jove.com)
This recombinant DNA was introduced into the worm using a microinjection, and the gene was expressed, similarly to β-tubulin. (jove.com)

It is due to mutations in the glucocerebrosidase gene (GBA) that catalyses the hydrolysis of glucosylceramide (GlcCer). (fundacionareces.es)

The long-term undifferentiation of molluscan sex chromosomes is potentially sustained by the unexpected intertwined regulation of reversible sex-biased genes, together with the lack of sexual dimorphism and occasional sex chromosome turnover. (nature.com)
The pleiotropic constraint of regulation of reversible sex-biased genes is widely present in ancient homomorphic sex chromosomes and might be resolved in heteromorphic sex chromosomes through gene duplication followed by subfunctionalization. (nature.com)
The regulation of HSP70 by HSF1 was preliminarily confirmed by binding site predictions and by a dual luciferase assay. (biomedcentral.com)
In order to study the regulation or IRE1 branch of the UPR, and to identify the ER-stress-modulating compounds, a human luciferase reporter cell line (XBP1-NLuc) was created in this work. (helsinki.fi)

The effects of PIP5K1α and its inhibitor on growth and invasion of BC were confirmed by using in vitro assays including proliferation, migration, apoptosis and luciferase reporter assays and in vivo xenograft mouse models. (lu.se)

Because the reporter enzymes themselves (e.g. firefly luciferase) can be direct targets of small molecules and confound the interpretation of HTS data, novel coincidence reporter designs incorporating artifact suppression have been developed. (wikipedia.org)
Mature miRNAs are generated by the RNase III enzymes Drosha and Dicer, then incorporate into the RNA-induced silencing intricate (RISC), and eventually bind to the 39-untranslated location (39-UTR) of their target gene mRNAs, inhibiting their expression[1,2]. (signsin1dayinc.com)
Aberrant expression of these two enzymes may result in epigenetic alteration and, consequently, dysregulated gene expression ( 10 , 11 ). (spandidos-publications.com)

A modified firefly luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo . (addgene.org)

In tests, the BV was loaded with green fluorescent proteins or firefly luciferase. (futurity.org)
In addition to its critical role in viral replication, PL pro removes post-translational modifications like ubiquitin and interferon-stimulated gene product 15 (ISG15) from host proteins through its deubiquitinase domain, leading to host immunosuppression and increased ability of the virus to evade the host antiviral immune response. (biorxiv.org)

Broccoli extracts, purified Sf, ascorbic acid and PA were tested for ability to induce thioredoxin reductase and quinone reductase transcription in luciferase reporter gene constructs. (usda.gov)

As expected clones varied in their specific luciferase activity along with the dilutions. (biotech-angels.com)
Examples of how SBI's BLIV lentivectors deliver strong luciferase activity after both transduction and transfection. (reportergene.com)
Examples of how SBI's BLIV minicircle vectors deliver strong luciferase activity in small animal models. (reportergene.com)
Examples of how SBI's BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity. (reportergene.com)
In conclusion, the XBP1-NLuc reporter cell line provided a reliable reporter of IRE1 endonuclease activity, whose expression is increased during the ER stress. (helsinki.fi)
Whereas the in vitro translation efficiency of these two mRNA isoforms was about equal, the mHIF-1alphaI.2 5'-untranslated region (5'-UTR) conferred significantly higher in vivo luciferase reporter gene activity than the mHIF-1alphaI.1 5'-UTR. (uzh.ch)
During pilocarpine-induced SE, in vivo disruption of STEP activity allowed activation of the MAPK pathway, leading to immediate-early gene expression and significant rescue from cell death. (jneurosci.org)
We characterized these viruses by comparing luciferase activity to virus production, and validated luciferase activity as an authentic real-time measure of viral growth. (genscript.com)
Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. (muni.cz)
Here, we introduce LuminoCell-an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. (muni.cz)

Many methods of transfection and transformation - two ways of expressing a foreign or modified gene in an organism - are effective in only a small percentage of a population subjected to the techniques. (wikipedia.org)
Luciferase measurement of a few unbiased transfection experiments was carried out in triplicate employing 15 ml of the supernatant according to the manufacturer's protocol. (signsin1dayinc.com)
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)

Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. (muni.cz)

Hypoxia inducible component 1 (HIF-1) is a heterodimeric transcription element that regulates transcription of genes this sort of as vascular endothelial expansion element (VEGF) and primary fibroblast development factor (bFGF) [fourteen,15,sixteen]. (signsin1dayinc.com)
MicroRNA (miRNA) belongs to the family of single-stranded noncoding RNA with approximately 22 nucleotides that negatively regulates the target genes at the posttranscriptional level, which is involved in a wide range of biological processes [ 11 ]. (hindawi.com)

As expected, ER stress treatment increased the reporter expression, while IRE1 inhibitors decreased the expression of the reporter. (helsinki.fi)
High-throughput, luciferase-based reverse genetics systems for identifying inhibitors of Marburg and Ebola viruses. (genscript.com)

The GUS gene has been commonly used in plants but luciferase and GFP are becoming more common. (wikipedia.org)

They are ideally not present in the native genome to be able to isolate reporter gene expression as a result of the gene of interest's expression. (wikipedia.org)
Note that lentivectors can be used to generate stable reporter cell lines as they integrate into the genome. (reportergene.com)

It is recommended to use medium-strength promoters (such as TK) rather than strong promoters (such as SV40, CMV), so as not to affect the expression of the main reporter gene, or to conduct experiments to find a suitable vector. (yeasenbiotech.com)

MicroRNAs (MiRNAs) are brief non-coding RNAs (182 nt), which inhibit gene expression. (signsin1dayinc.com)
MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
RNA interference is a conserved pathway common to plants and mammals, where double-stranded RNAs (dsRNAs) suppress expression of genes with complementary sequences [ 1-2 ]. (idtdna.com)

A：1) Firefly luciferase is recommended to choose pGL-3 or pGL-4 or a self-constructed vector. (yeasenbiotech.com)
2) Renilla luciferase is recommended to select pRL-TK or pGL-4 vector or construct the corresponding vector by yourself. (yeasenbiotech.com)

A set of three sensors that are increasingly structurally destabilized versions of Firefly luciferase which serves as a convenient toolkit to assess the proteostasis status in a wide range of experimental systems. (addgene.org)

In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression. (muni.cz)

Application of the magnetic field allows BV transduction, the payload-delivery process that introduces gene-editing cargo into the target cell. (futurity.org)

MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. (frontiersin.org)
HDACs and histone acetylases together regulate gene expression by histone acetylation and de-acetylation. (spandidos-publications.com)

We identified 150 genes responsive to heat shock including seven HSP genes, six of which belonging to the group of 17 HSP genes enriched in response to heat shock, according to weighted gene co-expression network analysis (WGCNA). (biomedcentral.com)
Many heat responsive genes were reported in these studies. (biomedcentral.com)

Reporter genes are often used as an indication of whether a certain gene has been taken up by or expressed in the cell or organism population. (wikipedia.org)
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. (wikipedia.org)
It is important to use a reporter gene that is not natively expressed in the cell or organism under study, since the expression of the reporter is being used as a marker for successful uptake of the gene of interest. (wikipedia.org)

A：at room temperature (20-22°C). Reaction components such as cell lysate, substrate working solution, etc. need to be adjusted to room temperature, because the reaction rates of the two luciferases are affected by temperature. (yeasenbiotech.com)
ATP Cell Viability Luciferase Assay A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability. (sigmaaldrich.com)
The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. (sigmaaldrich.com)
Store ATP Cell Viability Luciferase Assay at -80°C. Avoid repeated freeze-thaw cycles. (sigmaaldrich.com)
A new technology that relies on a moth-infecting virus and nanomagnets could edit defective genes that give rise to diseases like sickle cell, muscular dystrophy, and cystic fibrosis. (futurity.org)
That's big enough to transport more than 38,000 base pairs of DNA, which is enough to supply multiple gene-editing units to a target cell, Bao says. (futurity.org)
Targeted disruption of the gene encoding D4Rs reduces the amplitude of the contrast sensitivity rhythm by reducing daytime sensitivity and abolishes the rhythmic expression of Npas2 and Adcy1 mRNA in the ganglion cell layer (GCL) of the retina. (jneurosci.org)
This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. (cancerindex.org)
INDIGO Bioscience's nuclear receptor reporter assays are all-inclusive cell-based assay systems. (thomassci.com)

To activate reporter genes, they can be expressed constitutively, where they are directly attached to the gene of interest to create a gene fusion. (wikipedia.org)
The researchers use the magnetic nanoparticles to activate BV and deliver gene-editing payloads only where they're needed. (futurity.org)

Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons. (addgene.org)

A comprehensive understanding of gene expression in human RB is essential for the development of safe and effective new therapies. (molvis.org)

Subsequent verification using the reporter assay revealed that CORO1C is an indirect downstream target of YB-1. (biomedcentral.com)

Anatomy23

Organisms19

Diseases3

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes80

Technology, Industry, Agriculture1

Information Science3

Dual luciferase reporter gene4

Firefly luciferase and Renilla3

Gaussia4

Promoter15

Protein22

Encodes2

Differentially4

Plasmids6

Luminescence3

Plasmid3

Transcriptional3

Detection3

Cells22

RLuc2

Bacteria5

Visually identifiable characteristics1

Reagent1

Recombinant4

Mutations1

Regulation4

Vivo xenograft1

Enzymes3

Bioluminescence1

Proteins2

Constructs1

Activity10

Transfection3

Molecular biology1

Regulates2

Inhibitors2

Commonly1

Genome2

Experiments1

RNAs3

Vector2

Experimental1

Cytotoxicity1

Transduction1

Regulate2

Responsive2

Organism3

Cell9

Activate2

Catalyzes1

Understanding of gene expression1

Target1