Gene under selection. Medical search. Frequent questions

First, genes are clustered into gene groups with a clustering algorithm so that genes belonging to the same cluster have similar expression profile. (mun.ca)
GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
The recent advance in the microarray data analysis makes it easy to simultaneously measure the expression levels of several thousand genes. (hindawi.com)
In this work, we are interested in gene expression data dimension reduction for cancer classification, which is a common task in most microarray data analysis studies. (hindawi.com)
This technology developed in the early 1990s allowed researchers to simultaneously measure the expression levels of several thousand genes [ 1 , 2 ], These levels of expression are very important for the detection or classification of the specific tumor type. (hindawi.com)
A gene expression matrix composed with M samples and N genes. (hindawi.com)
Gene expression studies could be considered as one of the key experimental approaches for such purposes. (lu.se)
A suitable reference gene selection is fundamental for quantitative gene expression analysis and functional genomics studies in I. sexdentatus. (lu.se)
Twelve commonly used reference genes in Coleopterans were screened under different experimental conditions to obtain accurate and reliable normalization of gene expression data. (lu.se)
In contrast, different sets of genes were shown higher stability in terms of expression under tissue-specific conditions, i.e. (lu.se)
The findings were validated further using real-time quantitative reverse transcription PCR (RT-qPCR)-based target gene expression analysis. (lu.se)
Nevertheless, the present study delivers a catalog of reference genes under varied experimental conditions for the coleopteran forest pest I. sexdentatus and paves the way for future gene expression and functional genomic studies on this species. (lu.se)
Analyzing gene expression of PSGs during aging in the long-lived naked mole-rat and up-regulation in the short-lived rat, we found a pattern fitting the antagonistic pleiotropy theory of aging. (biorxiv.org)
The expression stability of these genes was analyzed using different algorithms (geNorm, NormFinder, and BestKeeper). (techscience.com)
To verify the reliability of the data, the expression of the glucose dehydrogenase ( gdh ) gene under different soluble phosphate levels was quantified using the most stably expressed reference gene. (techscience.com)
Therefore, this study provides a stable and reliable reference gene of Serratia for the accurate quantification of functional gene expression in future studies. (techscience.com)
This study reports a 22-bp nucleotide insertion-deletion (indel) genetic polymorphism that may be causally related to the control of gene expression of the fatty acid desaturases, enzymes that control the biosynthesis of long-chain polyunsaturated fatty acids (LCPUFA) from 18-carbon PUFA. (fatsoflife.com)
None of these small polymorphisms can be clearly assigned to changes in the amino acid sequence of the FADS enzymes, and are believed to mostly influence the regulation of gene expression. (fatsoflife.com)
This genetic variant, termed an indel (insertion/deletion mutation), was found to influence the regulation of the expression levels of both FADS-1 and FADS-2 in a cellular system, with the minor allele in Japanese people being associated with lower expression of the FADS-1 and FADS-2 genes. (fatsoflife.com)
Bertelsen A.H., Velculescu V.E., High-throughput gene expression analysis using SAGE, Drug Discovery Today 3 (1998) 152-159. (gse-journal.org)
Expressed Sequence Tag (EST) sequencing is one of the most efficient means for gene discovery and gene expression profiling. (auburn.edu)
We present SRGS, SPLS (sparse partial least squares)-based recursive gene selection, to infer GRNs from bulk or single-cell expression data. (biomedcentral.com)
When dealing with gene expression data with dropouts, we randomly scramble samples, set some values in the expression matrix to zeroes, and generate multiple copies of data through multiple iterations to make SRGS more robust. (biomedcentral.com)
It has been shown that SRGS is competitive with the existing GRN methods and effective in the gene regulatory network inference from bulk or single-cell gene expression data. (biomedcentral.com)
For microarray gene expression data, we first summarize solutions in dealing with 'large p, small n' problems, and then propose an integrative Bayesian variable selection (iBVS) framework for simultaneously identifying causal or marker genes and regulatory pathways. (escholarship.org)
Exploring Plasmodium falciparum Var Gene Expression to Assess Host Selection Pressure on Parasites During Infancy. (tropmedres.ac)
We hypothesized that, if maternal antibodies to VSA imposed a selection pressure on parasites, then the expression of a relatively conserved subset of var genes called group A var genes in infants should change with waning maternal antibodies. (tropmedres.ac)
The observed increase in group A var gene expression with age in the first year of life, when the maternal antibodies are waning and before acquisition of naturally acquired antibodies with repeated exposure, is consistent with the idea that maternally acquired antibodies impose a selection pressure on parasites that infect infants and may play a role in protecting these infants against severe malaria. (tropmedres.ac)
The wealth of gene expression values being generated by high throughput microarray technologies leads to complex high dimensional datasets. (biomedcentral.com)
This paper introduces a Balanced Iterative Random Forest (BIRF) algorithm to select the most relevant genes for a disease from imbalanced high-throughput gene expression microarray data. (biomedcentral.com)
The designed BIRF algorithm is an appropriate choice to select genes from imbalanced high-throughput gene expression microarray data. (biomedcentral.com)
The huge number of gene expression values generated by microarray technology leads to very complex datasets, and many cohorts have the imbalanced classes problem (e.g. 80% alive vs. 20% deceased). (biomedcentral.com)
Hence, feature selection is a critical technique in the field of bioinformatics [ 1 ] and it has been used in various domains for large and complex data, such as gene expression datasets. (biomedcentral.com)
Gene expression datasets are typically noisy and often consist of a limited number of observations (hundreds) relative to the large number of gene expression values (thousands of genes). (biomedcentral.com)
These characteristics result in difficulties in working with standard machine learning techniques, which must be modified to deal with the complexities of gene expression data and to build an effective feature selection algorithm. (biomedcentral.com)
techniques used for evaluating gene expression [1]. (scirp.org)
genes in gene expression studies of neutrophils [10]. (scirp.org)
90-100 million years, repeated losses, relaxed selection, and low expression highlighted the dispensability of SIT3, consistent with a model of deterioration and eventual loss due to relaxed selection on SIT3 expression. (lu.se)
The extensive but relatively balanced history of duplications and losses, together with paralog-specific expression patterns, suggest diatoms continuously balance gene dosage and expression dynamics to optimize silicon transport across major environmental gradients. (lu.se)
Movement of these genes from 1 organism to another or an increase in their expression, for example, can trigger a resistance phenotype ( 1 ). (cdc.gov)
Changes in THEMIS gene expression due to the rs138300818 variant promote the development of early-onset type 1 diabetes. (wikipedia.org)
How much are natural selection and gene characteristics, such as the number of protein-protein interactions (PPIs), tissue specificity (𝞽), and expression level, connected? (qxmd.com)
We observed that the variance of expression of tissue-specific genes seems not related to their level of selection constraints, which is the opposite of what is found on non-tissue-specific genes. (qxmd.com)
The expression stability of the candidate reference genes were analyzed using geNorm, NormFinder, Bestkeeper, Delta CT method, and RefFinder software packages. (researcher-app.com)
The six genes exhibiting less variation in each organism were evaluated in terms of their expression stability using geNorm, NormFinder and BestKeeper. (1library.org)
The comprehensive evaluation of gene expression patterns is an important part of this characterization and, at present, the quantitative real-time polymerase chain reaction (RT-qPCR) is considered the gold standard for measurement of transcript abundance [6]. (1library.org)
The advent of new methods to obtain large-scale surveys of various mathematical structures, such as systems of differen- gene expression in which transcript levels can be determined tial equations, graphical networks, stochastic functional re- for thousands of genes simultaneously has facilitated the ex- lations, and simulation models. (lu.se)
D-SWCNT, D-MWCNT, ASB, ultrafine carbon black (UFCB) and control SAEC genome profiles were subjected to comparative marker and class neighbor analyses followed by multistep cross validation to identify genes with highly correlated expression for each treatment. (cdc.gov)
Gene marker subsets and disease markers were validated using rtPCR and protein expression. (cdc.gov)
miRNAs are small noncoding RNAs that have broad regulatory functions on gene expression. (lu.se)
Changes in gene expression mediate growth and development in all living organisms. (lu.se)
Strong selection pressures experienced during one stage of development may differ from those experienced in other stages, and with consequences for the underlying patterns of gene expression. (lu.se)
We want to understand differences in life stage-specific gene expression in the peacock fly and relate these patterns to signatures of selection across the genome. (lu.se)
The student will analyze gene expression in a reference-based pipeline using existing RNA sequencing data from larvae, pupae, and adult flies from each of the two host forms. (lu.se)
This will include co-expression network analyses to identify groups of genes that are expressed similarly and might belong to the same or linked gene regulatory networks. (lu.se)
The student will evaluate gene expression in relation to measures of genetic diversity, differentiation, and neutrality across the genome to evaluate whether the sequences or the flanking regions of genes with stage-specific expression demonstrate signs of selection. (lu.se)
Selection for contextual fear conditioning affects anxiety-like behaviors and gene expression. (bvsalud.org)
Type a gene name with the keyboard to color graphs by the expression of that gene. (lu.se)
If index sorting has been used the graph can be colored by the expression of a selection cell surface marker using the menu. (lu.se)

Under developmental conditions, the Tubulin beta-1 chain (β-Tubulin) was the most stable reference gene, followed by translation elongation factor (eEF2) and ribosomal protein S3 (RPS3). (lu.se)
In 2012, a much larger, 22-base pair long stretch of nucleotides was identified within a part of the FADS-2 gene that is not translated to protein (an intron). (fatsoflife.com)
While the long-term goal of the SSPsyGene Consortium is to build a comprehensively annotated resource describing the CNS function of all neurodevelopmental and psychiatric disorder risk genes, the current target for the initiative is 100-250 protein coding genes. (nih.gov)
The goal of this initiative is to fill a very specific knowledge gap area, which is that many protein-coding genes are now associated with NPD risk, but their basic neurobiology remains poorly understood. (nih.gov)
Numerous factors shape the evolution of protein-coding genes, including shifts in the strength or type of selection following gene duplications or changes in the environment. (lu.se)
Positive selection results in frequent reversible amino acid replacements in the G protein gene of human respiratory syncytial virus. (bvsalud.org)
Protein THEMIS is a protein encoded by the eponymous THEMIS gene. (wikipedia.org)
This protein plays a regulatory role in both positive and negative T cell selection during late thymocyte development. (wikipedia.org)
pac is a gene that encodes the puromycin resistance protein, puromycin N-acetyltransferase. (toku-e.com)
Insights into gene tissue specificity and protein-protein interactions in the context of purifying selection in humans. (qxmd.com)
Nonsynonymous mutations change the protein sequences and are frequently subjected to natural selection. (biomedcentral.com)
In coding region of DNA, it is imaginable that the nonsynonymous mutations that change the protein sequences would undergo strong selection, and those nonsense mutations that cause pre-mature termination of protein synthesis are also highly deleterious and subjective to purifying selection. (biomedcentral.com)
This method allows variations in the protein-coding region of any gene to be identified, rather than in only a select few genes. (medlineplus.gov)
However, researchers have found that DNA variations outside the exons can affect gene activity and protein production and lead to genetic disorders--variations that whole exome sequencing would miss. (medlineplus.gov)
The student will be responsible for improving the annotations, especially for the untranslated regions that lie up and downstream of protein coding genes. (lu.se)

A notable challenge exists when dealing with the high dimensionality of the data sets, as thousands of genes or millions of genetic variants are collected on a relatively small number of subjects. (escholarship.org)

Although some tests were marginally significant for some populations and genes, none of them were significant after multiple-testing correction. (discovermagazine.com)
This is consistent with the theory that positive selection may play a role in the persistence of schizophrenia at a frequency of one per cent in human populations around the world, despite its strong effects on reproductive fitness and its high heritability from generation-to-generation. (sciencedaily.com)
Jointly, these two sets of data support the hypothesis that advantageous mutations within the FADS gene cluster occurred prior to human migration out of Africa (~85 kya), and swept to fixation within African but not European or Asian populations. (blogspot.com)
Here, we show evidence that these high efficiency converter alleles in the FADS gene cluster were likely driven to near fixation in African populations by positive selection ~85 kya. (blogspot.com)
We hypothesize that selection at FADS variants, which increase LC-PUFA synthesis from plant-based MC-PUFAs, played an important role in allowing African populations obligatorily tethered to marine sources for LC-PUFAs in isolated geographic regions, to rapidly expand throughout the African continent 60-80 kya. (blogspot.com)
Positive Selection of an Indel Polymorphism in the FADS Gene Cluster May be Driving Long-Chain PUFA Biosynthetic Capacity in Specific Human Populations. (fatsoflife.com)
Here, we studied whether the complete set of ZTGs are enriched for signals of positive selection in worldwide populations and population groups from South Asia. (upf.edu)
ZTGs showed higher levels of genetic differentiation between African and non-African populations than would be randomly expected, as well as other signals of polygenic selection outside Africa. (upf.edu)
Discharges of chemical agents (drugs, disinfectants, heavy metals, and other pollutants) into the environment can accelerate the lateral movement of resistance genes across bacterial populations by increasing selective pressure for maintenance of mobile genetic elements (MGEs) ( 4 ) or by increasing the rate of gene transfer ( 5 ). (cdc.gov)
Sure, it takes me more time to prepare culture media, but the tradeoff is that I have populations of high reporter-expressing cells that were a lot less work to make than if I had done clonal selection. (imanislife.com)
The role of natural selection in shaping adaptive trait differentiation in natural populations has long been recognized. (rug.nl)
Evidence of Early-Stage Selection on EPAS1 and GPR126 Genes in Andean High Altitude Populations. (ox.ac.uk)
With the advent of NGS technique, the explosion of NGS data generated from the tumor tissues help researchers identify driver mutations in cancer-related genes, but relatively less attention is paid to the SNP data in healthy human populations when studying cancer. (biomedcentral.com)

Selecting relevant genes that are involved with a cancer is a challenging task. (mun.ca)
this is carried out by selecting a small subset of relevant genes and eliminating the redundant or noisy genes. (hindawi.com)

We obtained genomic and transcriptomic data from 17 rodent species and systematically scanned eleven lineages associated with the evolution of longevity and eusociality for positively selected genes (PSGs). (biorxiv.org)
Most of the lost genes were found to have moved to other genomic regions through segmental duplications, suggesting an evolutionary trend of centromere-linked genes escaping from centromeric regions. (plantae.org)
Therefore it is not surpris- pansion of biological understanding from the analysis of in- ing that the advent of high-throughput genomic and pro- dividual genes to the analysis of systems of genes (and pro- teomic technologies is drawing a growing interest from the teins). (lu.se)

However, to obtain reliable RT-qPCR results the use and validation of reference genes is mandatory. (1library.org)
To our knowledge, this is the first work on the validation of reference genes for cyanobacteria constituting a valuable starting point for future works. (1library.org)

Similarly, only considering the number of mutations within the haplotype group D1, the TMRCA was 85,000±84,000 years, thus suggesting that selection in Africa occurred approximately 85 kya. (blogspot.com)
To maximize the potential disease relevance, genes with a genome-wide significant excess burden of loss-of-function mutations in NPDs such as intellectual disability/developmental delay, autism spectrum disorder, and schizophrenia are the focus. (nih.gov)
Many deleterious mutations may then be harmless, because even if one gene suffers a mutation, the redundant gene copy can provide a back-up function. (biomedcentral.com)
By simply dividing the human genes into cancer-related genes and other genes, we compared the features of nonsynonymous, synonymous and nonsense mutations in these two gene sets from multiple aspects. (biomedcentral.com)
We provided lines of evidence that the nonsynonymous, synonymous and nonsense mutations in cancer-related genes undergo stronger purifying selection when compared to the expected pattern in other genes. (biomedcentral.com)
The synonymous SNPs, after excluding those in splicing regions, exhibit preferred changes in codon usage and higher codon frequencies in cancer-related genes compared to other genes, indicating the constraint exerted on these mutations. (biomedcentral.com)
Nonsense mutations are less frequent and located closer to stop codons in cancer-related genes than in other genes, which putatively minimize their deleterious effects. (biomedcentral.com)
Our study demonstrated the evolutionary constraint on mutations in CDS of cancer-related genes without the requirement of data from cancer tissues or patients. (biomedcentral.com)
Our work provides novel perspectives on interpreting the constraint on mutations in cancer-related genes. (biomedcentral.com)
We reveal extra constraint on synonymous mutations in cancer-related genes which is related to codon usage bias and is in addition to the splicing effect. (biomedcentral.com)
However, the selection on synonymous mutations for a long time was thought to be negligible or very weak. (biomedcentral.com)
Thus, it is comparably important to study the role of synonymous mutations that affect the codon usage bias in cancer-related genes. (biomedcentral.com)

This workshop introduces the functional metagenomic selections as a tool to identify known and novel antibiotic resistance gene from complex clinical and environmental communities. (dtu.dk)
SSPsyGene is designed to provide a collaborative and efficient framework for identifying biological function beyond current small-scale and often ad hoc single gene efforts in order to generate a standardized, experimentally derived, functional catalog of NPD risk genes. (nih.gov)
Traditional gene-wise selection methods using univariate analyses face difficulty to incorporate correlational, structural, or functional structures amongst the molecular measures. (escholarship.org)
A novel partial least squares (PLS) g-prior for iBVS is developed to allow the incorporation of prior knowledge on gene-gene interactions or functional relationships. (escholarship.org)
The male sterilizing factors are produced by recombination of the mitochondrial genome and consist of unusual genes or open reading frames (ORFs) that usually contain a portion of functional mitochondrial genes and sequences of unknown origin ( Hanson and Bentolila, 2004 ). (frontiersin.org)
eling require a broad range of signal processing approaches, Two salient goals of functional genomics are to screen for including signal representation relevant to transcription and key genes and gene combinations that explain specific cel- system modeling using nonlinear dynamical systems. (lu.se)

In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)

In order to evaluate the proposed method, we tested MWIS-ACO-LS on ten well-replicated microarray datasets of high dimensions varying from 2308 to 12600 genes. (hindawi.com)
These characteristics of the microarray data make it very likely the presence of redundant or irrelevant genes, which limit the performance of classifiers. (hindawi.com)
Thus, extracting a small subset of genes containing valuable information about a given cancer is one of the principal challenges in the microarray data analysis [ 4 ]. (hindawi.com)
The main motivation of this selection is to identify and select the useful genes contained in a microarray dataset for distinguishing the sample classes. (hindawi.com)

nonetheless, until now, no validated reference genes from the genus Serratia have been reported that can be used for the normalization of quantitative real-time polymerase chain reaction (RT-qPCR) data. (techscience.com)
In general, TUBB3 was consistently the least stable gene for normalization. (researcher-app.com)
In addition, the minimum number of reference genes required for normalization was determined. (1library.org)
Based on the three algorithms, we provide a list of genes for cyanobacterial RT-qPCR data normalization. (1library.org)

Cancer datasets normally contain a small size of samples while each sample has a large number of genes as features. (mun.ca)
In the cancer classification task, a small number of samples are available, while each sample is described by a very large number of genes. (hindawi.com)

Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria. (1library.org)
Citation: Pinto F, Pacheco CC, Ferreira D, Moradas-Ferreira P, Tamagnini P (2012) Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria. (1library.org)

The parallel analysis of ESTs from the two closely related ictalurid catfishes should also provide powerful means for the evaluation of ancient and recent gene duplications, and for the development of high-density microarrays in catfish. (auburn.edu)
We compiled SITs from 37 diatom genomes to characterize shifts in selection following gene duplications and marine- freshwater transitions. (lu.se)
Episodic diversifying selection was detected but not associated with gene duplications or habitat shifts. (lu.se)
The excess gene loss is consistent with selective loss of the parental gene copy in dynamic centromeric region after gene duplications and supports the antagonistic relationship between the centromere environment and gene survival. (plantae.org)
This hypothesis originated as least as early as Ohno's seminal book [ 1 ], which emphasized the importance of gene duplications in organismal evolution. (biomedcentral.com)

Long time readers will be familiar with the large literature in behavior genetics/genomics and dopamine receptor genes. (discovermagazine.com)

Mechanisms involved include modification of the drugs to less toxic derivatives or avoidance of their action by exclusion, target modification, and mutation of target-associated genes. (cdc.gov)
Once a person affected with tuberous sclerosis complex is found to have a mutation in the TSC1 or TSC2 gene, at-risk family members may be tested. (medscape.com)

Feature selection as one of the methods of dimensionality reduction is used for selecting a subset of features which lead to a better performance of the classifier. (mun.ca)
In wrapper methods, the selection can be seen as an exploration of all the possible subsets, and the principle is to generate a subset of genes and evaluate it afterward. (hindawi.com)
Generally, the accuracy according to the final subset of genes is high because of the bias of the process of generating the classifier used. (hindawi.com)
While it is anticipated that assays should aim to characterize 100-250 genes, some phenotypes and assay formats may be more amenable to moderate scale screening against a subset (50-100) of the gene set. (nih.gov)
ADGCs supported in Phase 2 of the initiative will be involved in continued efforts to refine and expand the list, as well as developing a rigorous, data-informed approach for selecting patient variants in allelic series from a subset of NPD risk genes. (nih.gov)
Particularly for moderate throughput assays, strategies for selecting a subset of genes from the list should be discussed in the context of the chosen assay(s) or readout(s). (nih.gov)

For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR) is the standard technique. (1library.org)

Genes belonging to proteases, bacterial exporters and DNA stabilization were found to be under strong positive selection, thus facilitating pathogenicity and survival of the outliers. (frontiersin.org)
Me and two friends thought about your question during our Biology BSc at ICL (in fact we also thought about an even more puzzling phenomenon called "bacterial transformation" which is like conjugation, but they incorporate genes from dead bacteria! (stackexchange.com)

The inter- and intra- specific SNPs identified from all catfish EST dataset assembly will greatly benefit the catfish introgression breeding selection and whole genome association studies. (auburn.edu)
A deep gene duplication, which coincided with a whole-genome duplication, gave rise to two gene lineages. (lu.se)
Put differently, after gene duplication - which can arise through polyploidization (whole-genome duplication), non-homologous recombination, or through the action of retrotransposons - one or both duplicates should experience relaxed selective constraints that result in elevated rates of evolution. (biomedcentral.com)
While many more genetic changes can be identified with whole exome and whole genome sequencing than with select gene sequencing, the significance of much of this information is unknown. (medlineplus.gov)
GeneReviews compares whole genome sequencing, whole exome sequencing, and the sequencing of a selection of individual genes , particularly their use in the diagnosis of genetic conditions. (medlineplus.gov)
The project will rely on published whole genome assemblies and gene annotations of various qualities. (lu.se)

Our findings show that ecDNA can develop early in the transition from high-grade dysplasia to cancer, and that ecDNAs progressively form and evolve under positive selection. (nature.com)
However, centromeres may evolve from gene-containing regions and active genes have indeed been found within centromeric regions. (plantae.org)
It will be interesting to investigate how new genes evolve and fix at pericentromeric regions. (plantae.org)
Does one gene evolve more rapidly than the other? (biomedcentral.com)
Several recent genome-wide studies have suggested that duplicate genes are always under purifying selection and do not always evolve at the same rate. (biomedcentral.com)

Question: Is the list of genes available? (nih.gov)

From the point view of systems biology, iBVS enables user to directly target the joint effects of multiple genes and pathways in a hierarchical modeling diagram to predict disease status or phenotype. (escholarship.org)
The first of these, the follistatin gene, is expressed in the developing feather bud and found in pathways with genes that determine the structure of feathers and may thus be important in generating variation in structural colouration. (rug.nl)
Two of these genes, arginase 1 and eotaxin 1 are the focus of continuing investigations in mouse models of asthma regarding differences in mechanistic pathways depending on the allergen. (cdc.gov)
Array studies provide candidate genes to identify common mechanistic pathways in the effector phase, as well as mechanistic pathways unique to individual allergens. (cdc.gov)

Virulence genotype analysis revealed that strain CR1 lacked hemolytic phospholipase C and D, three genes for LPS biosynthesis and had reduced antibiotic resistance genes when compared with clinical strains. (frontiersin.org)
We will explore the options for antibiotic resistance gene annotations, showing participant how they can broadly annotate hundreds of antibiotic resistance genes from different data input and perform detailed BLAST analysis in CARD, Resfam and Pfam. (dtu.dk)
Antimicrobial drug resistance is caused by microbial gene products that attenuate the activity of an antibiotic in an otherwise drug-sensitive organism. (cdc.gov)
thus, resistance genes may be latent under certain conditions, only to be activated in others. (cdc.gov)
Well, my personal favorite is the puromycin resistance gene. (imanislife.com)
If we consider that a bacterium is another bacterium's rival (in terms of obtaining resources) why should a bacterium "share" it's antibiotic resistance gene, for example, with another bacterium? (stackexchange.com)

2018). Comparison of Oryza sativa and Oryza brachyantha Genomes Reveals Selection-Driven Gene Escape from the Centromeric Regions. (plantae.org)
In addition, there may be species-specific differences in K a /K s , but detection of such differences is sensitive to how information on gene duplicates is extracted from genomes and on how K a and K s are estimated. (biomedcentral.com)

Which antibiotic selection gene? (imanislife.com)
I grow a lot of reporter cell lines, and most of them are under antibiotic selection. (imanislife.com)
And even if you don't end up using antibiotic selection, having the selection gene in your cells usually won't impact your study. (imanislife.com)
Doing the kill curve will help you establish a baseline for how much antibiotic is required to kill cells that aren't expressing the selection gene. (imanislife.com)
For reporter cell lines with selection genes, I recommend maintaining the cells in antibiotic at a concentration that kills greater than 95% of parental cells. (imanislife.com)
Well, for selection I recommend using a higher concentration of antibiotic. (imanislife.com)

Given the complex genetic nature of the condition, selection may be mediated by a diverse array of neurodevelopmental, neurophysiological and psychological mechanisms. (sciencedaily.com)
The researchers analysed the molecular evolution of the 76 genes that have the strongest genetic association with the disorder. (sciencedaily.com)
Understanding the impact of positive selection may also help refine hypotheses concerning genetic links between schizophrenia and aspects of human creativity and cognition. (sciencedaily.com)
Over many generations, the selection of beneficial traits associated with particular polymorphisms can lead to gradual changes in the frequency of specific genetic polymorphisms in a genetically-isolated population. (fatsoflife.com)
In contrast with the positive selection of favorable adaptive polymorphisms, a genetic variant may also gradually disappear from a population if there is no survival advantage in having it. (fatsoflife.com)
Studies on polymorphisms found in one cluster of closely-located genes coding for fatty acid desaturases (FADS), enzymes that play an important role in the biosynthesis of both omega-6 and omega-3 LCPUFA, are providing an interesting perspective on the interaction between traditional diets and the population genetic history of humans. (fatsoflife.com)
In order to identify novel molecular targets of tumor cell migration and invasion we performed a genetic screen with an shRNA library against ubiquitination pathway-related genes. (nature.com)
This zeitgeist-y innovation is the subject of a new Netflix series, Unnatural Selection, from film-makers Joe Egender and Leeor Kaufman, and explores the various forms of genetic engineering, as well as the societal and environmental implications of its research and use. (herokuapp.com)
With genetic engineering, Dr Kevin Esvelt, an evolutionary engineer profiled in the series, hopes to foster a process called gene drive in rodents, a technology designed to transmit a particular suite of genes throughout a population through breeding. (herokuapp.com)
Although, there was a significant difference between polygenic and major gene genetic response between two methods, after the 5th generations, but there was no significant difference for the sum of polygenic and major gene response. (asabe.org)
The aim of this study is to identify genetic variants that harbour signatures of recent positive selection and may facilitate physiological adaptations to hypobaric hypoxia. (ox.ac.uk)
Few in vivo studies identified whole lung gene markers associated with MWCNT exposure, but did not compare CNT vs. asbestos genetic response. (cdc.gov)

With this kind of dataset, biologists need to identify a small number of informative genes that can be used as biomarkers for a disease. (biomedcentral.com)

Biological networks can be used to functionally annotate genes on the basis of interaction-profile similarities. (umn.edu)

Table 1: A selection of the most cited therapeutics and genes in implementation studies in PGx PHGKB as of March 21, 2022. (cdc.gov)
The Ihsan Doramaci Family Health Foundation Prize Selection Panel met on 24 January 2022 to consider the nominations of the five candidates, together with the Administrator's technical comments on the candidatures. (who.int)

Application of Approximate Bayesian Computation Markov chain Monte Carlo based to these sequence data using a simple forward simulator revealed broad posterior distributions of the selective parameters for all four genes, providing no support for positive selection. (discovermagazine.com)
However, the selective forces influencing the evolution of these genes remain unknown. (sciencedaily.com)
In humans, some zinc transporter genes (ZTGs) have been previously reported as candidates for strong geographically restricted selective sweeps. (upf.edu)
The associated genes often offer some broader selective advantage and consequently have been mobilized and horizontally transferred to other microorganisms sharing their ecologic habitat. (cdc.gov)
Our study showed that selective forces conferred by centromere chromatin dynamics may drive gene relocation out of centromeric regions. (plantae.org)
In other words, the duplicate genes are under few or no selective constraints. (biomedcentral.com)
The smaller the K a /K s number of eliminated substitutions and the greater the selective constraint under which the two genes have evolved. (biomedcentral.com)
Even very closely related gene duplicates, no older than a few million years, experience selective constraints - the ratio K a /K s is smaller than one even in these cases. (biomedcentral.com)

This is the first study to describe a systematic comprehensive selection of reference genes for murine extramedullary hematopoietic tissues over a developmental time course. (researcher-app.com)
We provide suggested reference genes for individual tissues and developmental stages and propose that a combination of reference genes affords flexibility in experimental design and analysis. (researcher-app.com)

Of these unique sequences, over 35% had significant similarities to known genes by BLASTX searches, which allowed the identification of 14,776 unique genes in the catfish. (auburn.edu)

The results showed that the zipA and 16S rRNA genes were the most stable reference genes, and the least stable genes were thiC and recA . (techscience.com)
A total of 1,194 potential lactic acid bacterium (LAB) isolates were tentatively grouped and identified by repetitive element sequence-based PCR, followed by sequence-based identification using 16S rRNA and pheS genes from a selection of genotypically unique LAB isolates. (who.int)
In parallel, all samples were analyzed by denaturing gradient gel electrophoresis (DGGE) of V3-16S rRNA gene amplicons. (who.int)

Micro- array data from the ovalbumin and TMA model of asthma were also compared to previous data using Aspergillus as allergen to identify putative asthma `signature genes', i.e. genes up-regulated with all 3 allergens. (cdc.gov)

This method decreases the computational complexity and redundancy of genes which leads to a better classification accuracy. (mun.ca)

In sex-specific conditions, RPS3, β-Tubulin, and eEF2 were the most stable reference genes. (lu.se)

And how frequent is gene conversion of duplicate genes, in which recombination and DNA repair between very similar genes convert the sequence of one to that of the other? (biomedcentral.com)

Applying all commonly used neutrality-test statistics for allele frequency distribution to the newly generated sequence data provided conflicting results regarding evidence for positive selection. (discovermagazine.com)
However, when we applied this approach to published sequence data on SLC45A2, another human pigmentation candidate gene, we could readily confirm evidence for positive selection, as previously detected with sequence-based and some haplotype-based tests. (discovermagazine.com)
The Scalable and Systematic Neurobiology of Psychiatric and Neurodevelopmental Disorder Risk Genes (SSPsyGene) Consortium: Assay and Data Generation Centers (RM1) (RFA-MH-24-145). (nih.gov)
The resulting phenotypic data will be integrated across modalities, levels of organization, and genes to create a harmonized, integrated knowledge base needed to substantively advance our understanding of basic gene function and, ultimately, make robust inferences into potential shared and unique disease mechanisms. (nih.gov)
A Gene Selection Task Group within the SSPsyGene Consortium has initiated efforts to prioritize a set of ~100-250 genes associated with NPDs to be interrogated by all Assay and Data Generation Centers (ADGCs), the list of which can be found on the "Resources" tab of the SSPsyGene Consortium website operated by the UC Santa Cruz Data Resource and Administrative Coordinating Center (DRACC). (nih.gov)
Array data are intended to provide candidate genes for hypothesis generation and further experimentation. (cdc.gov)

Such frequent evolutionary reversals in HRSV are indicative of a combination of frequent positive selection, reflecting the changing immune status of the human population , and a limited repertoire of functionally viable amino acids at specific amino acid sites. (bvsalud.org)
If a duplicate gene pair shows a K a /K s ratio of about 1, that is, if amino-acid replacement substitutions occur at the same rate as synonymous substitutions, then few or no amino-acid replacement substitutions have been eliminated since the gene duplication. (biomedcentral.com)
The lower nonsynonymous to synonymous ratio observed in cancer-related genes suggests the suppression of amino acid substitutions in these genes. (biomedcentral.com)

PCR-RFLP was used to genotype 87 animals of Iranian Sarabi cattle for A and B alleles of kappa casein gene. (asabe.org)

The second is a gene potentially underlying the ability to detect this variation: SWS1 opsin. (rug.nl)
But the variation across gene pairs is huge. (biomedcentral.com)

2] In this latter case we could hypothetically see a discontinuous sort of negative frequency-dependent selection, with variants that were "too successful" getting periodically wiped out. (gnxp.com)
We identified two missense variants as significant targets of selection. (ox.ac.uk)
One of these variants, located within the GPR126 gene, has been previously associated with the forced expiratory volume/forced vital capacity ratio. (ox.ac.uk)

Roca-Umbert A, Caro-Consuegra R, Londono-Correa D, Rodriguez-Lozano GF, Vicente R, Bosch E. Understanding signatures of positive natural selection in human zinc transporter genes. (upf.edu)

Several SNPs located in the FADS gene cluster are now known to be related to changes in risk for complex and chronic diseases. (fatsoflife.com)

So depending on the type of in vivo study you have planned, you may want to skip the selection gene. (imanislife.com)

Our finding that positive evolutionary processes have impacted genes underlying the disorder is consistent with this idea. (sciencedaily.com)
How and to what extent have centromere locations changed and what is the evolutionary fate of genes within centromeric and pericentromeric regions? (plantae.org)

From our screen, we identified the Ubiquitin-specific protease 19 (USP19) as a candidate gene associated with the regulation of cell migration. (nature.com)
Continued studies focused on genes showing minimal changes in the TMA-induced model but with clear up-regulation in the ovalbumin model. (cdc.gov)

Here, we search for signals of selection in candidate genes for colour and its perception in a passerine bird. (rug.nl)
Organ-level analysis demonstrated that HPRT1 was the most suitable reference gene for heart, liver and thymus samples, while TBP was the best candidate for spleen samples. (researcher-app.com)
Towards this goal we have selected and analyzed twelve candidate reference genes from three morphologically distinct cyanobacteria grown under routinely used laboratory conditions. (1library.org)
EPAS1 is known to be the major selection candidate gene in Tibetans. (ox.ac.uk)
Different statistical methods with varying selection criteria resulted in identification of similar candidate genes. (cdc.gov)

Based on these observations, previous studies have hypothesized that positive selection has acted on the DRD4 7R allele. (discovermagazine.com)
In this study, to re-examine the possibility of recent positive selection favoring the DRD4 7R allele, we genotyped HapMap subjects for DRD4 VNTR, and conducted several neutrality tests including long range haplotype test and iHS test based on the extended haplotype homozygosity. (discovermagazine.com)
Thus, we conclude that the DRD4 7R allele has not been subjected to strong recent positive selection. (discovermagazine.com)
Researchers found a higher prevalence of the influence of so-called positive selection on genes or gene regions known to be associated with the disorder than a comparable control set of non-associated genes, functioning in similar neuronal processes. (sciencedaily.com)
Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection. (ox.ac.uk)
Our study replicated some well-characterized targets for positive selection in East Asia and sub-Saharan Africa, and proposes new candidates for follow-up in South Asia (SLC39A5) and Africa (SLC39A7). (upf.edu)

The identification of gene regulatory networks (GRNs) facilitates the understanding of the underlying molecular mechanism of various biological processes and complex diseases. (biomedcentral.com)

In this project, we conduct a comprehensive study by examining widely used clustering, feature selection, and classification methods, and applying them to different cancer datasets including Colon, Leukemia and, Lymphoma. (mun.ca)

Anatomy10

Organisms25

Diseases2

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment50

Psychiatry and Psychology13

Phenomena and Processes120

Disciplines and Occupations8

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture5

Humanities1

Information Science14

Health Care29

Expression59

Protein15

Thousands of genes1

Populations13

Relevant genes2

Genomic3

Validation of reference genes2

Mutations12

Functional6

Proteins2

Microarray4

Normalization4

Large number of genes2

Suitable reference genes2

Duplications5

Genomics1

Mutation2

Subset6

Quantitative1

Bacterial2

Whole-genome6

Evolve5

List of ge1

Pathways4

Resistance6

Genomes2

Antibiotic6

Genetic12

Dataset1

Annotate1

20222

Selective8

Tissues2

Sequences1

RRNA3

Putative1

Redundancy1

Stable1

Recombination1

Data6

Amino acid3

Alleles1

Variation2

Variants3

Signatures of positive1

SNPs1

Skip1

Evolutionary2

Regulation2

Candidate5

Positive selection6

Regulatory1

Datasets1