• For outgoing non-human material transfers (i.e. mice, modified cell lines, reagents, etc.), please refer the Office of Technology Management (OTM) website or e-mail [email protected] . (ucsf.edu)
  • Examples of such research material include physical materials such as tissue samples, transgenic mice, cell lines, gene constructs, compounds, antibodies, as well as computer software and data. (ucsf.edu)
  • Methods: To address this question, transgenic Non-Obese Diabetic (NOD) mice which express insulin in the liver were made using the Phosphoenolpyruvate Carboxykinase (PEPCK) promoter to drive the mouse insulin I gene (Ins). (deepdyve.com)
  • The transgenic PEPCK-Ins NOD mice became diabetic at 20-25 weeks of age, with blood glucose levels of 24.1 ± 1.7 mmol/l. (deepdyve.com)
  • Haematoxylin and eosin staining of liver sections from these transgenic NOD PEPCK-Ins mice revealed the absence of an infiltrate of immune cells, a feature that characterised the pancreatic islets of these mice. (deepdyve.com)
  • person to synthesise, store and secrete insulin in the same manner as a pancreatic β cell is a potential therapy of type The liver of the transgenic PEPCK-Ins NOD mice were 1 diabetes. (deepdyve.com)
  • transplanted into diabetic mice these cells can lower blood glucose levels to the normal range [2]. (deepdyve.com)
  • Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. (cshlpress.com)
  • This video guide complements Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition and Transgenic Techniques in Mice: A Video Guide . (cshlpress.com)
  • How are genes inserted into mice? (promisekit.org)
  • Transgenic mice are made by using glass micropipettes to inject a solution that contains DNA from a chosen source into the nucleus of a fertilized mouse egg. (promisekit.org)
  • How transgenic mice can be produced? (promisekit.org)
  • To do this they genetically-engineered mice by adding a fluorescent gene from fireflies to them. (promisekit.org)
  • Which is the first method used to produce the transgenic mice? (promisekit.org)
  • Why are mice used for transgenic animals? (promisekit.org)
  • Such work laid the basis for the creation of transgenic mice genetically modified to inherit particular forms of cancer. (promisekit.org)
  • The double transgenic mice (dTg) were obtained by mating: (i) transgenic mice expressing the hemagglutinin of influenza virus under the insulin promoter with (ii) transgenic mice expressing specific T lymphocytes with receptor for the immunodominant epitope of the same virus. (promisekit.org)
  • Human NK cells developed in hIL-2 NOG mice express various NK receptors and produce both granzyme A and perforin upon stimulation. (taconic.com)
  • Founders were backcrossed to NOG mice to establish the hIL-2 NOG line. (taconic.com)
  • Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. (taconic.com)
  • at the 1986 ISIR-TNO Annual Meeting, September 7-12, 1986, reported the production of transgenic mice carrying mouse interferon beta or gamma genes coupled to the mouse MT-I or heat shock promoters. (justia.com)
  • Apparently, Kawade had not determined whether the chimeric gene was actually expressed in the mice, but had already been able to express it in cultured cells induced by Cd.sup.2+ or by heat. (justia.com)
  • one of the mice was transgenic. (justia.com)
  • Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. (jax.org)
  • 2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
  • I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)
  • However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)
  • Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)
  • The transfer of lymphocytes or ethylene di-isocyanate (HDI), which are used in a serum from sensitized mice can cause clinical disease in naive variety of industries including polyurethane foam mice. (cdc.gov)
  • We observed a significant increase in IFN-γ-producing CD8 + T cells in bronchoalveolar lavage fluid (BALF) of immunocompetent mice that repeatedly aspirated A. fumigatus conidia in contrast to mice challenged with A. versicolor , a species that is not typically associated with invasive, disseminated disease. (cdc.gov)
  • Airway IFN-γ + CD8 + T-cells were decreased and lung germination was eliminated in mice that aspirated A. fumigatus conidia that were formaldehyde-fixed or heat-inactivated. (cdc.gov)
  • Furthermore, A. fumigatus particles exhibited greater persistence in the lungs of recipient mice when compared to non-viable A. fumigatus or A. versicolor , and this correlated with increased maintenance of airway memory-phenotype CD8 + T cells. (cdc.gov)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • During embryogenesis, ISWI was found to interact with select ArsI sites throughout the genome, and when inactivated led to misregulation of select gene expression, loss of insulator activity and aberrant morphogenesis. (biologists.com)
  • Chromatin insulators are DNA sequences that function in the epigenetic regulation of gene expression, including three-dimensional structural alterations of the chromatin. (biologists.com)
  • These findings shed light on the regulation of gene expression in guard cells and provide new promoter modules as useful tools for manipulating gene expression in guard cells, both for physiological studies and future biotechnological applications. (biomedcentral.com)
  • this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
  • Our results suggest that regulation of gene expression in sensory neurons can function in the integration of a wide array of sensory information and facilitate decision-making behaviors in C. elegans . (elifesciences.org)
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • STAT4 also signals activation of the TF T-bet, a lineage-defining factor for Th1 differentiation, which upregulates the IL-12 receptor, IFN-γ expression, and causes further expansion of Th1 cells (Figure 1 ) ( 12 ). (frontiersin.org)
  • In general, information derived from microarray databases such as Arabidopsis eFP Browser ( http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi ) and Genevestigator Gene Chronologer ( https://www.genevestigator.com/gv/index.jsp ) support experimental data on ACBP expression obtained from investigations using Northern blot analysis and reverse-transcription-polymerase chain reaction. (aocs.org)
  • These two cell types differed, however, in their NF-κB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. (ersjournals.com)
  • In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
  • Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. (cshlpress.com)
  • Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
  • These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)
  • Viral expression of wild type α-Synuclein caused accumulation of α-Synuclein and was associated with increased cell death and inflammation. (biomedcentral.com)
  • Parkin co-expression reduced the levels of phosphorylated α-Synuclein and attenuated cell death and inflammation. (biomedcentral.com)
  • Biochemical regulation of gene expression. (entrance-exam.net)
  • The gene encodes the interferon of a different animal species and therefore its expression product is less toxic to the host at an embryonic, fetal, neonatal or junvenile stage of development. (justia.com)
  • Expression was not reported, and the purpose of the study was to show that the technique could be used to study gene action during mammalian development. (justia.com)
  • Contrary to expectations, a fragment containing the entire region between the TBPH gene and the bgcn translational start drove strong HEG expression only during late spermatogenesis rather than in the germline stem cells and spermatogonia as expected. (plos.org)
  • While transformation procedures have become routine for a growing number of plant species, the random introduction of complex transgenenic DNA into the plant genome by current methods generates unpredictable effects on both transgene and homologous native gene expression. (bioone.org)
  • GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
  • The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. (lu.se)
  • Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. (lu.se)
  • There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
  • These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
  • 2000). We of glial cell line-derived neurotrophic factor (GDNF) in models of have recently reported that this vector directs transgene expression to Parkinson's disease (Georgievska et al. (lu.se)
  • An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)
  • Materials and methods approach to regulate transgenic expression. (lu.se)
  • This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)
  • The upcoming objective is certainly to apply these cells for the creation of transgenic lamb by somatic cell nuclear transfer technique. (researchensemble.com)
  • The retinal cells in your eyes use proteins called opsins to detect incoming light, which in turn enable you to see. (laserglow.com)
  • Thus, stress response studies during these growth stages reveal novel differentially regulated genes or proteins with important functions in plant stress adaptation. (intechopen.com)
  • In plants cells, lipid-transfer proteins (LTPs) and acyl-CoA-binding proteins (ACBPs) have been identified as candidates for lipid transfer within the cell [reviewed in 1,2]. (aocs.org)
  • To test the potential role of parkin in modulating α-Synuclein post-translational modifications (i.e. ubiquitination and phosphorylation) and toxicity, we used lentiviral gene transfer animal models, which allow us to examine the in vivo effects of these proteins. (biomedcentral.com)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
  • This paper examines the safety implication of recent revelations on the recombination hotspot of the cauliflower mosaic viral (CaMV) promoter, which is in practically all current transgenic crops released commercially or undergoing field trials. (i-sis.org.uk)
  • As a precautionary measure, we strongly recommend that all transgenic crops containing CaMV 35S or similar promoters which are recombinogenic should be immediately withdrawn from commercial production or open field trials. (i-sis.org.uk)
  • All products derived from such crops containing transgenic DNA should also be immediately withdrawn from sale and from use for human consumption or animal feed. (i-sis.org.uk)
  • That is all the more urgent as CaMV promoter is in practically all transgenic crops already released commercially or undergoing field trials. (i-sis.org.uk)
  • Early indications suggest that gene editing should be regulated like conventionally bred crops, as the final product can simply match what may be done by conventional breeding. (ufl.edu)
  • This will enable transgenic versions of soybean, corn and potato crops, or even ornamental plants, to be used in the future for large-scale production of medical drugs. (fapesp.br)
  • The solution found by the NIH team, led by O'Keefe, was to seek out Professor Elíbio Rech at Embrapa, the coordinator of the Brazilian group that had filed an international patent for a gene insertion technique for soybeans, and who had experience in developing transgenic crops. (fapesp.br)
  • Agricultural scientists are also attempting to develop insect-resistant transgenic crops by using insecticidal toxin genes derived from Photorhabdus spp ( 4 ). (cdc.gov)
  • Using human embryonic stem cells requires COMS registration and approval by the Embryonic Stem Cell Research Oversight Committee. (harvard.edu)
  • To generate a transgenic mouse, microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert the gene of interest into a zygote or into embryonic stem cells. (promisekit.org)
  • A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. (cshlpress.com)
  • Scientist Phil L'Huillier who is heading the project, say's the first two or three years will be spent putting transgenic embryos into cows and breeding from them to produce transgenic calves which will in turn breed to produce herds of up to 30 animals for milking. (jesus-is-savior.com)
  • Many protocols possess been set up for sexing the cell and embryos lines in farm pets. (researchensemble.com)
  • As a consequence, cell fate acquisition and subsequent differentiation of endosperm tissues are affected to varying degrees of severity. (silverchair.com)
  • Finally, a number of biologics that target CD4 + T cell differentiation into inflammatory subsets or their byproducts (i.e., cytokines) have shown efficacy in treating patients with IBD ( 5 - 7 ). (frontiersin.org)
  • Interleukin (IL)-12, which is secreted by antigen-presenting cells, acts via signal transducer and activator of transcription (STAT)4 to promote the differentiation of naïve T cells into Th1 cells ( 9 - 11 ). (frontiersin.org)
  • Critical factors in the differentiation of effector Th cells during inflammatory bowel disease (IBD). (frontiersin.org)
  • CD80/86-CD28 interaction and others), these signals initiate a program of cell division and differentiation. (frontiersin.org)
  • Interleukin (IL)-12 and IL-23, cytokines induced during early stages of IBD, play important roles in differentiation of interferon (IFN)-γ/tumor necrosis factor (TNF)-producing Th1 cells as well as IL-17-producing Th17 cells. (frontiersin.org)
  • Predominant differentiation of human NK cells following human HSC engraftment, with>10-fold higher CD56+ NK cell numbers compared to the base NOG mouse. (taconic.com)
  • May be an improved model for differentiation/engraftment of IL-2 dependent cells including certain blood cancers. (taconic.com)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • Two common approaches for transferring this behavior are germline transgenesis and somatic gene delivery . (laserglow.com)
  • Gordon and Ruddle, Science, 214:1244 (1981) claimed integration and stable germline transmission of genes injected into mouse pronuclei. (justia.com)
  • In particular, HEG gene drive could be particularly effective if activity was restricted to the male germline to target genes required for female fertility/viability or engineered to destroy the X-chromosome by cutting at multiple X-specific sites [5] , [6] . (plos.org)
  • Creating transgenic animals and plants. (harvard.edu)
  • Based on policy discussions, it is possible that the first-generation plants containing the edited genes will not require extensive regulation, and these tools will be extremely valuable in the long-term efforts of every strawberry breeding program. (ufl.edu)
  • The first step is to develop and optimize a tissue culture and transformation system (protocols to introduce foreign DNA to new plants) for UF strawberry lines, so that new plants can be regenerated from cells containing introduced DNA. (ufl.edu)
  • GUS reporter fusions in transgenic plants we were able to demonstrate a modular organization for the AtMYB60 promoter. (biomedcentral.com)
  • Dozens of experiments by companies and academic institutions all over the world are using techniques to insert genes in the genomes of plants, enabling them to start producing enzymes that have pharmacological value. (fapesp.br)
  • Seventy-five percent (6 out of 8) of the edited events tested remained asymptomatic in comparison to the control non-edited plants under water stress conditions, confirming inhibition of eBSV and reversal of its ability to be converted into infectious viral particles in the edited lines. (nature.com)
  • There are many plants that produce blue flowers in nature, but our research team first chose a petunia exhibiting a dark violet color in order to isolate blue genes. (suntory.com)
  • Therefore, to reduce the time required to obtain results, we decided to introduce the candidate genes of blue genes to yeast instead of plants and test their enzymatic activities in yeast. (suntory.com)
  • There are several methods to introduce genes into plants. (suntory.com)
  • Because this bacterium has the ability to carry its own genes into plant cells, it is used in the gene transfer of many plants. (suntory.com)
  • Future research programs can be focused on the development of transgenic plants with enhanced stress tolerance in field conditions based upon the outcome of genomic approaches and knowing the mystery of nucleotides sequences hidden in cells. (intechopen.com)
  • There is lack of consideration for a large segment of the population that has ethical or religious beliefs that classify genetically engineered plants carrying animal or human genes as being totally unacceptable as foods. (jesus-is-savior.com)
  • The six Arabidopsis ACBPs are subcellularly located in various compartments based on experimental results using a variety of techniques including fluorescent protein tagging in confocal microscopy, immunoelectron microscopy and western blot analysis of subcellular fractions from wild-type plants as well as from transgenic plants expressing fluorescent-tagged ACBPs. (aocs.org)
  • Application of genetic engineering in improving many maize genotypes is, however, limited by lack of suitable regeneration systems that result in normal and fertile plants from the in vitro grown transformed cells. (scialert.net)
  • Current trends and future prospects for optimization of excision-activation machinery and its practical implementation for the generation of transgenic plants and plant products free of undesired genes are discussed. (bioone.org)
  • The myosin-binding protein C, cardiac-type is a protein that in humans is encoded by the MYBPC3 gene. (wikipedia.org)
  • cMyBP-C is a myosin-associated protein that binds at 43 nm intervals along the myosin thick filament backbone, stretching for 200 nm on either side of the M-line within the crossbridge-bearing zone (C-region) of the A band in striated muscle. (wikipedia.org)
  • The other project involves the study of a single nucleotide polymorphism (SNP) in the human p53 gene that results in either arginine (R) or proline (P) at position 72 of the p53 protein. (mdanderson.org)
  • 2009) and by Meyer et al (2010), who used this promoter to complement the mrp5-1 mutant phenotype exclusively in guard cells, and to specifically express the AtLMT12 protein at high levels in guard cells, respectively. (biomedcentral.com)
  • The first described CID systems involve microbe-derived dimerizing ligands, such as rapamycin, that direct protein neo-associations that rewire eukaryotic cell signaling 5 . (nature.com)
  • The active principle of the drug in question is taliglucerase alpha, a protein produced by transgenic carrot cells for the treatment of Gaucher's disease, a rare genetic condition caused by a lack of glucocerebrosidase, an enzyme our bodies need to process glucocerebrosides, a type of cellular fat. (fapesp.br)
  • Up until now, Gaucher's Disease was treated with a different drug, whose active protein is produced by hamster cells modified through a biotechnological process that is more vulnerable to contamination. (fapesp.br)
  • The NIH attempted a production method using recombinant DNA, in which the gene that encodes the protein is inserted in the genome of Escherichia coli (which is more easily grown in the laboratory) for subsequent extraction of the desired substance. (fapesp.br)
  • Western blotting and quantitative real-time reverse transcription PCR (qRT-PCR) were used to determine protein and gene levels. (bvsalud.org)
  • Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma. (kanazawa-u.ac.jp)
  • Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
  • February 12, 1987) observed inhibition of influenza virus in NIH 3T3 mouse cells genetically modified to express the mouse protein Mx. (justia.com)
  • Staehelin suggested that an animal could be protected against viral infection by inserting a gene encoding the Mx protein into that animal, preferably at the single cell embryo stage. (justia.com)
  • He taught that the gene of choice would be the one encoding the Mx protein that is normally found in the species of animal to be protected. (justia.com)
  • When a HEG is integrated into its recognition sequence in the genome, its protein product acts to cleave its cognate site on the homologous chromosome and gene conversion or homologous recombination can result in a new copy of the HEG being inserted. (plos.org)
  • In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green ¯uorescent protein (GFP)-expressing cells. (lu.se)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
  • The CaMV promoter is a sequence of about 350 basepairs upstream of the 35S transcript (-343 to +8, with Cap site at +1), about 250 basepairs of which overlap with the 3' end of gene Vl, the last of the six large open reading frames. (i-sis.org.uk)
  • These studies show that the loss of up to 40 amino acids from the 3' end of gene Vl (which overlaps with the 35S promoter) had no effect on pathogenesis whereas further truncation into a putative zinc finger region was fatal to the virus. (i-sis.org.uk)
  • Enhancer-blocking activities include the repression of gene transcription as a result of the insulator being located between an enhancer and the promoter of that gene. (biologists.com)
  • Guard cell specificity of the AtMYB60 promoter has been also demonstrated by Nagy et al . (biomedcentral.com)
  • 9] has recently showed that adenovirus-medi- Materials ated in vivo transfer of the PDX-1 transgene to the mouse The PEPCK promoter was generously donated by Dr R. W. liver results in the conversion of a hepatocyte subpopula- Hanson (Case Western Reserve University, Cleveland, tion to the beta cell phenotype. (deepdyve.com)
  • However, the fundamental issue whether insulin-secreting hepatocytes in vivo will be destroyed by the autoimmune processes that kill pancreatic β cells has not been fully addressed. (deepdyve.com)
  • Results: The liver cells were found to possess preproinsulin mRNA, translate (pro)insulin in vivo and release it when exposed to 100 nmol/l glucagon in vitro. (deepdyve.com)
  • Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
  • However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
  • Additionally, our data suggest that acquisition of aleurone cell fate does not solely rely upon signalling from the maternal surrounding tissue to the periphery of the endosperm, as previously thought, but that other factor(s) present within the endosperm are involved. (silverchair.com)
  • IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
  • CD4 + T cells are enriched in lesional tissue from patients with CD and UC and blockade or depletion of CD4 + T is effective in treating patients with IBD. (frontiersin.org)
  • Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
  • Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
  • Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)
  • In the absence of mature B cells, patients lack lymphoid tissue and fail to develop plasma cells, the cells that manufacture antibodies. (medscape.com)
  • Germinal centers where B cells proliferate and differentiate are poorly developed in all lymphoid tissue, including the spleen. (medscape.com)
  • Therefore, murine airway CD8 + T cell-responses to aspiration of Aspergillus conidia may be mediated in part by the ability of conidia to germinate in the host lung tissue. (cdc.gov)
  • Viral titers in ferret nasal wash specimens and nasal turbinate tissue correlated positively with peak titer in Calu-3 cells, whereas additional phenotypic and molecular determinants of influenza virus virulence and transmissibility in ferrets varied in their association with in vitro viral titer measurements. (cdc.gov)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
  • However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
  • Latest research have got demonstrated that fetal skeletal muscles satellite television cells possess a versatile potential to end up being utilized for transgenic pet creation by somatic cell nuclear transfer technique because these cells are muscle-derived control cells that can possibly expand and differentiate. (researchensemble.com)
  • To usage of fetal transgenic satellite television cells for nuclear transfer Prior, sex recognition of transgenic cell lines singled out from one cell cloning is certainly required because the gender of transgenic 217087-09-7 manufacture embryo can end up being motivated by sex recognition of nuclear donor cells. (researchensemble.com)
  • MN9 D cells were maintained in Dulbecco's modified Eagle's medium (Sigma, D5648) containing 10% fetal bovine serum (FBS) and hygromycin B (200 μg/mL). (biomedcentral.com)
  • Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
  • The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
  • [ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
  • Mutations can occur in any area of the gene. (medscape.com)
  • If this is the case, gene-correction presents a solution for patients with known monogenetic mutations. (lu.se)
  • Foreign gene sequences could be inserted into deletion mutants, which may alter the infectious characteristics of the virus. (i-sis.org.uk)
  • Background This study was performed to explain the basic methods to isolate and culture of primary satellite cells (PSCs) obtained from 50 to 60-day-old sheep fetuses, single cell cloning of transfected sexing and PSCs of PSCs based on the ZFY/ZFX, amelogenin and high-motility-group (HMG) box sequences. (researchensemble.com)
  • Burt proposed that such methods could be applied to engineer HEGs that recognise and cleave sequences within coding sequences of genes in insect genomes, with the subsequent invasion of these HEGs into a population leading to the inactivation of target genes and the subsequent decline in fitness of the targeted population [2] . (plos.org)
  • The signal in question is molecule called DAF-7, which is released by several sensory neurons-nerve cells that are used for detecting cues from the environment. (elifesciences.org)
  • CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
  • This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
  • Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
  • This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
  • The faculty and staff of the Georgia Esoteric and Molecular (GEM) Core provide diagnostic services for the diagnosis and comprehensive evaluation of disease primarily through the molecular analysis of tissues and cells. (augusta.edu)
  • Also, satellite television cells offer a steady model for tissues system research, such as those regarding the transplantation of muscle-derived satellite television cells for muscles tissues renovation [2]. (researchensemble.com)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • The use of viral vectors to deliver foreign genes to the brain is highly logical states in the human brain (Eng et al. (lu.se)
  • For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
  • By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)
  • Transgenic technology' refers to the transfer of a genetic material from one species to another. (ufl.edu)
  • Page 1 of 12 (page number not for citation purposes) Journal of Autoimmune Diseases 2004, 1:3 http://www.jautoimdis.com/content/1/1/3 would be unsatisfactory to address the issue of insulin Background Genetic alteration of non-pancreatic cells in a diabetic autoantigenicity. (deepdyve.com)
  • They gave us the genetic sequence that encodes the gene, and we inserted it in the genome of Embrapa's soybean variety 10-16. (fapesp.br)
  • To resolve misconfigured cells we include a Bayesian fitted online artificial that is a different restriction to perform the genetic and the yeast genes, Qbeing P-splines. (scoutconnection.com)
  • Hence there is a need to diversify the genetic basis of the locally adapted germplasm by introgressing genes responsible for improving tolerance to drought stress. (scialert.net)
  • The homing endonuclease (HEG) gene drive system is one proposed genetic strategy [2] . (plos.org)
  • Lateral transfer of genetic material between Photorhabdus and Yersinia species is thought to have resulted from their common association with insects as bacterial pathogens ( 5 ). (cdc.gov)
  • Genetic polymorphism studies demonstrated that the can develop immediate, late or dual asthmatic reactions glutathione-S-transferase gene family is associated with after chemical exposure. (cdc.gov)
  • The clinical C31 online artificial coverage treatments enriched for time Integrate have aimed from the Eukaryotic transgenic vertebrate scan Purification accuracy HEK293 and CHO microRNAs, which are from artifacts of the observed Hamster. (scoutconnection.com)
  • The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
  • The use of cell lines as "biological minipumps" to chronically deliver antinociceptive molecules into the pain-processing centers of the spinal cord represents a newly developed technique for the treatment of pain [ 3 ]. (hindawi.com)
  • On Monday May 13, 2013, the Supreme Court issued a unanimous decision in Bowman v. Monsanto Inc. , a closely-watched case with implications for patents on various types of technologies with "self-replicating" features, such as man-made cell lines, DNA molecules, certain nanotechnologies, and transgenic animals. (wolfgreenfield.com)
  • Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)
  • Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition . (cshlpress.com)
  • The other was to 'develop the methods to introduce these genes to cells of roses and produce genetically modified roses from these cells. (suntory.com)
  • Packaging cell PA317/TK was successfully constructed, and we acquired SW1990/TK through virus progeny infection. (wjgnet.com)
  • Interestingly, IBD patients with concurrent HIV infection also exhibit a greater incidence of remission as compared to non-HIV controls, correlating with decreased total blood CD4 + T cell counts ( 3 , 4 ). (frontiersin.org)
  • We compiled data from ferrets inoculated with an extensive panel of over 50 human and zoonotic IAV (inclusive of swine-origin and high- and low-pathogenicity avian influenza viruses associated with human infection) under a consistent protocol, with all viruses concurrently tested in a human bronchial epithelial cell line (Calu-3). (cdc.gov)
  • We show that certain viral titer measurements following infection of a human bronchial epithelial cell line are positively correlated with viral titers in specimens collected from virus-inoculated ferrets and employ mathematical modeling to identify commonalities between viral infection progression between both models. (cdc.gov)
  • In these studies, CD4 + T cell-depleting and blocking antibodies caused remission from disease in a number of CD and UC patients examined, suggesting a prominent role of CD4 + T cells in propagating disease ( 1 , 2 ). (frontiersin.org)
  • Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
  • Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
  • This type of experiment gained momentum in May 2012 when the United States Food and Drug Administration (FDA) issued its first-ever approval for a pharmaceutical drug produced by genetically engineered plant cells, allowing its commercial use to treat human patients. (fapesp.br)
  • Germ Line Germ line refers to the sex cells (eggs and sperm) that sexually reproducing organisms use to pass on their genomes from one generation to the next (parents to offspring). (promisekit.org)
  • Transcription occurs from a nonintegrated, circular minichromosome in the nucleus of the plant cell, and virion DNA is synthesised in the cytoplasm by reverse transcription of the 35S RNA transcript (4, 5). (i-sis.org.uk)
  • The hepatocyte has been suggested as a suita- characterised with respect to insulin mRNA transcription, ble target cell for such gene therapy [1-9]. (deepdyve.com)
  • Given the importance of CD4 + Th cells in the disease process, this review will focus on how Th cells differentiate in the inflamed intestinal tract during IBD and how the Th lineage-specific cytokines and transcription factors (TFs) contribute to disease. (frontiersin.org)
  • The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
  • 4] Porcine skin has been grafted onto burn patients,[5] and pig neuronal cells have been transplanted into patients with Parkinson (Parkinson's) disease and Huntington (Huntington's) disease. (medscape.com)
  • Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)
  • We tested how these two genes interact by examining the effects of parkin on post-translational modification of α-Synuclein in gene transfer animal models, using a lentiviral gene delivery system into the striatum of 2-month old male Sprague Dawley rats. (biomedcentral.com)
  • This ®nding will lead to the development of lentiviral vectors with autoregulatory capacities that may be very useful for gene therapy to the brain. (lu.se)
  • In this study we used a lentiviral vector that drives transgenic populations (BloÈmer et al. (lu.se)
  • Katano I, Takahashi T, Ito R, Kamisako T, Mizusawa T, Ka Y, Ogura T, Suemizu H, Kawakami Y, Ito M. (2015) Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouse. (taconic.com)
  • These approaches include cotransformation with two independent T-DNAs or plasmid DNAs followed by their subsequent segregation, transposon-mediated DNA elimination, and most recently, attempts to replace bacterial T-DNA borders and selectable marker genes with functional equivalents of plant origin. (bioone.org)
  • In the absence of functional Btk, mature B cells that express surface Ig and the marker CD19 are few to absent. (medscape.com)
  • Th1 cells are important for protecting against infectious pathogens. (frontiersin.org)
  • These cells primarily produce interferon (IFN)-γ and tumor necrosis factor (TNF) that, respectively, activate macrophages and direct cytotoxic CD8 + T cell responses, that in turn promote elimination of intracellular pathogens such as viruses and bacteria ( 8 ). (frontiersin.org)
  • Christian Conze, Nataliya Trushina and Roland Brandt (2023) Molekulare Medizin: Live cell imaging zeigt, wie Tau den axonalen Transport ausbremst. (uni-osnabrueck.de)
  • The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
  • Recently, research has increased in the area of transplanting embryonic cells across species and growing kidneys and endocrine pancreas cells in situ. (medscape.com)
  • Various approaches to avoid or eliminate undesirable transgenes, most notably selectable marker genes used in plant transformation, have recently been developed. (bioone.org)
  • in comparison, muscle-derived satellite television cells research are uncommon in animals, such as lamb and cows. (researchensemble.com)
  • Our results offer an fresh basis for the comprehensive analysis and program of satellite television cells in various other areas, such as animals mating. (researchensemble.com)
  • This invention relates to the production of virus-resistant transgenic animals. (justia.com)
  • No evidence of tumour formation was observed in the transplanted animals, a major concern when utilising a pluripotent cell source. (lu.se)
  • Transgenic animals have identified a role for specific manufacturing, auto body painting and repair and immunity, including the involvement of T-helper type 1/2 plastics manufacturing. (cdc.gov)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
  • 1] Animal experimentation is going on all over the world the genes from chicken, cows, rats and humans have been engineered into commercial fish species in order to increase the growth rates- GM salmon, for instance, have been engineered to reach six times their natural size. (jesus-is-savior.com)
  • Our invention relates to the introduction of the interferon gene of another animal species into a host animal, and to the production of a healthy transgenic animal with enhanced viral resistance which it can genetically transmit to its offspring. (justia.com)
  • He did not suggest use of the interferon gene, or of a gene derived from a different species than the host. (justia.com)
  • Chan Y-S, Huen DS, Glauert R, Whiteway E, Russell S (2013) Optimising Homing Endonuclease Gene Drive Performance in a Semi-Refractory Species: The Drosophila melanogaster Experience. (plos.org)
  • The risk of transgene transfer into related plant species and consumers is another concern associated with the conventional transformation technologies. (bioone.org)
  • This term emerged out of the standardized longitudinal observation of pre-symptomatic carriers of autosomal dominant genes for ALS within the pre-fALS study, a natural history observational study. (medscape.com)
  • Homing endonuclease gene (HEG) drive is a promising insect population control technique that employs meganucleases to impair the fitness of pest populations. (plos.org)
  • We also observed that the fraction of targets undergoing homing was temperature-sensitive, falling nearly four-fold when the temperature was lowered to 18°C. Taken together, this study demonstrates how a few simple measures can lead to substantial improvements in the HEG-based gene drive strategy and reinforce the idea that the HEG approach may be widely applicable to a variety of insect control programs. (plos.org)
  • We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
  • The interactions with the various BM components differentially affect β-cells, although the underlying mechanism is largely unknown. (nature.com)
  • Pincikova T, Flodström-Tullberg M, Hjelte L. Cystic Fibrosis bronchial epithelial cells have impaired ability to activate vitamin D. Acta Paediatr. (karolinska.se)
  • Glucocorticoids can affect Pseudomonas aeruginosa (ATCC 27853) internalization and intracellular calcium concentration in cystic fibrosis bronchial epithelial cells. (karolinska.se)
  • Thus, we hypothesized that pericytes regulate β-cells through the production of BM components. (nature.com)
  • The endoscopy tableaux of the donor invention and the non-transgenic simple cohorts are with the network network convergence and θ administrator was. (scoutconnection.com)
  • Proper engineering of stomatal responses requires the use of guard cell-specific promoters, or the identification of guard cell-specific mutants, to avoid undesirable side effects on plant growth and productivity. (biomedcentral.com)
  • The sre Gene( ORF469) has a Site-Specific Recombinase Responsible for Integration of the R4 Phage Genome, 1996 J. Transgene approximating by the online artificial photosynthesis receptor functionality: substances for the cytoplasm of several IntechOpen data in promoters and bacteria, 2000 Plant Mol. (scoutconnection.com)
  • This review focuses on recent reports demonstrating the elimination of undesirable transgenes (essentially selectable marker and recombinase genes) from the plant genome and concomitant activation of a silent transgene (e.g., a reporter gene) mediated by different site-specific recombinases driven by constitutive or chemically, environmentally or developmentally regulated promoters. (bioone.org)
  • Early investigations were focused on the 10-kDa ACBPs from mammals and yeast, and from these studies the 10-kDa ACBP has been implicated in biological roles ranging from acyl-CoA transport, maintenance of acyl-CoA pools within the cell and in the protection of acyl-CoAs in the cytosol from enzymes including acyl-CoA hydrolase, acetyl-CoA carboxylase and acyl-CoA synthetase [reviewed in 3]. (aocs.org)
  • This suggests that CaMV evolved subsequent to the horizontal transfer of a retrotransposon to the cruciferae, either as the result of capture of RTgene by a pre-existing virus or by the transposable element acquiring additional genes to become a virus. (i-sis.org.uk)
  • We can utilize established DNA marker-assisted breeding tools to track the edited genes in subsequent generations, adding to the speed of new variety development. (ufl.edu)
  • Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner. (hindawi.com)
  • Before the announcement in February 1997 of the cloning of a sheep by somatic cell nuclear transfer, existing legislation in a number of countries already precluded human cloning for reproductive purposes, sometimes implicitly. (who.int)
  • based role shuffling of truncated and clinical observed genes: large amounts and women: time-dependent systems for the several using of different and baculovirus-dependent h0tis are Alternatively recently taken for a heterologous logarithmic estimationAfter and a longitudinal uptake example. (scoutconnection.com)
  • Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
  • Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
  • The use of site-specific recombination to remove undesired DNA from the plant genome and concomitantly, via excision-mediated DNA rearrangement, switch-activate by choice transgenes of agronomical, food or feed quality traits provides a versatile "transgene maintenance and control" strategy that can significantly contribute to the transfer of transgenic laboratory developments into farming practice. (bioone.org)
  • Inflammatory bowel disease is a complex set of diseases that includes Crohn's disease (CD) and ulcerative colitis (UC), each with multiple bacterial, immune, and non-immune cell types contributing to inflammation. (frontiersin.org)
  • During cellularisation,and at later stages of development, aberrant cell division and localised domains of cell proliferation are apparent in glo1-1 endosperms. (silverchair.com)
  • In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
  • E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
  • Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
  • Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
  • The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
  • May be useful for efficacy studies involving antibody therapeutics with antibody dependent cell cytotoxcity mechanism of action. (taconic.com)
  • Roundup Ready" refers to soybeans with a "chimeric" gene that protects them from common herbicides. (wolfgreenfield.com)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • The vascular BM surrounds the islet capillary network and directly contacts mouse endocrine cells. (nature.com)
  • Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
  • The eggs are then transferred to a foster female mouse. (promisekit.org)
  • What is a transgenic mouse line? (promisekit.org)
  • The CIEA NOG mouse ® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. (taconic.com)
  • mouse: an excellent recipient mouse model for engraftment of human cells. (taconic.com)
  • A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. (justia.com)
  • Preferably, the human beta interferon gene is introduced into the cells of a mouse. (justia.com)
  • Transgenic mouse models pro- fection. (who.int)
  • Monographs , transgenic mouse tients. (who.int)
  • Together with previous reports that proinsulin is a possible autoantigen in the development of Type 1 diabetes, the autoimmune destruction of insulin producing liver cells is a distinct possibility. (deepdyve.com)
  • Such cells made (pro)insulin content and (pro)insulin release. (deepdyve.com)
  • nylon membrane for the Southern Blot and ribonuclease protection assay (RPA) was from Amersham International For this gene therapy approach to be viable, the insulin (Bucks, UK). (deepdyve.com)
  • Human insulin and rat insulin standards for producing liver cells must not be destroyed by the the in-house radioimmunoassay (RIA) were obtained immune system or else this could lead to liver damage. (deepdyve.com)
  • This fundamental question has not been addressed by previous studies with (pro)insulin producing hepatocytes Transient transfection of HEPG2 cells The transfection of the HEP G2 cells with the PEPCK-Ins as diabetes in these models was induced chemically rather than by autoimmune means [6-9]. (deepdyve.com)
  • Gaussian online artificial photosynthesis in Human Cells Catalyzed by Phage polynucleotide Integrase Mutants, 2000 J. Maeser and Kahmann, The Gin model of event Mu can promote supernatant health in spline pairs, 1991 Mol. (scoutconnection.com)
  • Finally, we succeeded in isolating blue genes on June 13, 1991. (suntory.com)
  • This approach is known as 'CRISPR' gene editing. (ufl.edu)
  • We anticipate that, over the next decade, CRISPR and other gene editing techniques will be used to rapidly develop elite strawberry varieties with improved disease resistance, fruit quality, and other valuable attributes. (ufl.edu)
  • What is CRISPR gene editing? (ufl.edu)
  • CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is one form of gene editing that can be used to precisely modify a gene of interest without otherwise compromising the favorable traits of an elite variety (Rani et al. (ufl.edu)
  • Does CRISPR gene editing = transgenic technology? (ufl.edu)
  • We published another article about CRISPR gene editing in crop improvement ( https://edis.ifas.ufl.edu/publication/HS1334 ). (ufl.edu)
  • Twenty-eight pups were born, four of whom carried the chimeric IFN genes. (justia.com)
  • Egg and sperm cells are called germ cells, in contrast to the other cells of the body, which are called somatic cells. (promisekit.org)