• Validation experiments with knockdown of GAB2 and Far-Western blots proved the direct interaction of SHP2 with p85, independent of adaptor proteins and transfected FLAG-p85 provided evidence that SHP2 binding on p85 occurred on the SH2 domains. (sdbonline.org)
  • Noonan syndrome and related disorders are caused by mutations in genes encoding for proteins of the RAS-ERK1/2 signaling pathway, which affect development by enhanced ERK1/2 activity. (sdbonline.org)
  • Structural basis for phosphotyrosine recognition by the Src homology-2 domains of the adapter proteins SH2-B and APS. (genscript.com)
  • Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1). (rndsystems.com)
  • Endophilin-A2, SH3 domain protein 2B or SH3 Domain-Containing GRB2-Like Protein 1 is encoded by the gene Sh3gl1 and a member of the endophilin family of Src homology 3 domain-containing proteins. (antibodiesinc.com)
  • The ECM proteins bind to the extracellular domains of integrin heterodimers, whereas the actin stress fibers link to integrin cytoplasmic tails via large molecular complexes. (rupress.org)
  • Tensin is a gene family with four members (tensin1, tensin2, tensin3, and cten), and their encoding proteins are localized to the cytoplasmic side of focal adhesions. (rupress.org)
  • Here, we show that insulin-induced activation of ERKs was enhanced by stable overexpression of growth factor receptor-bound protein 2 (GRB2) but not by overexpression of GRB2 proteins with point mutations in the Src homology 2 and 3 domains. (nih.gov)
  • The SH2 domain binds to phosphotyrosine residues in RTKs such as PDGF and EGF, non-RTKs such as Bcr/Abl and FAK, and docking proteins such as FRS-2 and Gab1. (rndsystems.com)
  • Formins are multidomain proteins defined by a conserved FH2 (formin homology 2) domain with actin nucleation activity preceded by a proline-rich FH1 (formin homology 1) domain. (biomedcentral.com)
  • In vitro, the FH2 domain competes with barbed-end capping proteins and is necessary and sufficient to nucleate actin polymerization, but the FH1 domain, which interacts with profilin-actin, funnels actin to the nucleation vicinity and confers full activity to the molecule [ 1 ]. (biomedcentral.com)
  • The FH1 domain is also a binding site for diverse SH3-domain containing proteins like Src-like non-receptor tyrosine kinases, WISH (WASP-interacting SH3 protein) and IRSp53 (insulin receptor substrate) in mammals, and Hof1p in yeast [ 6 ]. (biomedcentral.com)
  • An important consequence of reversible phosphorylation of tyrosine residues on proteins is the creation of binding sites for phosphotyrosine recognizing domains such as Src homology 2 (SH2) and phosphotyrosine binding (PTB) domains [ 1 , 2 ]. (biomedcentral.com)
  • There are 47093 RhoGAP domains in 46979 proteins in SMART's nrdb database. (embl-heidelberg.de)
  • Taxonomic distribution of proteins containing RhoGAP domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with RhoGAP domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing RhoGAP domain in the selected taxonomic class. (embl-heidelberg.de)
  • Rho-specific GAP domains are found in a wide variety of large, multi-functional proteins. (embl-heidelberg.de)
  • CAPN3 provides some exclusive domains including its NH2-terminal domains I which has 20C30 additional proteins not within - and m-calpains and two exclusive insertion sequences' of 62 and 77 proteins on the COOH-terminal parts of domains II (known as Is normally1) and domains III (known as IS2). (exposed-skin-care.net)
  • Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • This protein contains the following domains, which are often found in intracellular kinases: N-terminus - PH (pleckstrin homology domain) TH - Tec family homology domain (including Bruton's tyrosine kinase Cys-rich motif and Proline rich region) SH3 - (Src homology 3) SH2 - (Src homology 2) C-terminus - tyrosine kinase, catalytic domain ITK (gene) has been shown to interact with: FYN, Grb2 and KHDRBS1, KPNA2, LAT, LCP2, PLCG1, PPIA, and WAS. (wikipedia.org)
  • PAG3 encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. (novusbio.com)
  • PIP3 binds to the pleckstrin homology (PH) domains of PKB, recruits it to the membrane where it is phosphorylated and converted to its activated form. (justia.com)
  • Gene expression of Sh3d19, a novel adaptor protein with five Src homology 3 domains, in anagen mouse hair follicles. (atlasgeneticsoncology.org)
  • GRB2 (growth factor receptor-bound protein 2), an adaptor protein involved in signal transduction, contains a central SH2 domain flanked by two SH3 domains. (rndsystems.com)
  • SHP-2 can hamper the TRIF (TIR-domain-containing adapter-inducing interferon-β) adaptor protein-dependent TLR4 and TLR3 signal transduction with a consequent block of the pro-inflammatory cytokine production [ 7 ]. (biomedcentral.com)
  • c-Src tyrosine kinase plays an important role in signal transduction pathways, where its activity is regulated by phosphorylation of the two tyrosine residues. (intechopen.com)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • In contrast to the activated epidermal growth factor receptor that binds the GRB2-Sos complex directly, activation of the insulin receptor results in the interaction of GRB2-Sos with IRS-1 and Shc, thus linking the insulin receptor to Ras signaling pathways. (nih.gov)
  • The SH3 domains associate with proline rich motifs on the guanine nucleotide releasing factor, Sos, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathways. (rndsystems.com)
  • Tyrosine phosphorylation of GRB2 SH3 domains reduces binding to Sos and negatively regulates downstream signaling pathways including Ras, JNK and MAPK. (rndsystems.com)
  • CRKL or v-crk sarcoma virus CT10 oncogene homolog (avian)-like is a protein kinase containing SH2 and SH3 (src homology) domains that activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion (1). (rockland.com)
  • Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. (rndsystems.com)
  • These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). (rndsystems.com)
  • Mutations on DLC-1 perturb the interaction with cten and disrupt the focal adhesion localization of DLC-1. (rupress.org)
  • Here we report the characterization of a new gene on the long arm of the X-chromosome (position Xq12) and the identification in unrelated individuals of different mutations that are predicted to cause a loss of function. (embl-heidelberg.de)
  • Characterization of novel Bruton's tyrosine kinase gene mutations in Central European patients with agammaglobulinemia. (lu.se)
  • Four novel and three recurrent mutations of the BTK gene and pathogenic effects of putative splice mutations. (lu.se)
  • Mutations in the STAT3 gene cause most cases of AD-HIES. (medscape.com)
  • In 2007, autosomal dominant mutations in signal transducer and activator of transcription-3 ( STAT3) gene were identified as the molecular cause of this disease. (medscape.com)
  • STAT3 gene is diagrammed with depiction of hotspots (areas where higher numbers of patients were noted to have mutations). (medscape.com)
  • Mutations in the STAT3 gene have been identified in almost all cases of clinically verified autosomal dominant hyper-IgE syndrome (AD-HIES). (medscape.com)
  • Increased curiosity about CAPN3 was highly stimulated when it had been reported that mutations in its gene bring about limb-girdle muscular dystrophy (LGMD) type 2A, seen as a the continuous atrophy of hip and make muscle tissues (LGMD2A, or calpainopathy) (21). (exposed-skin-care.net)
  • As opposed to other styles of muscles dystrophy, where mutations take place in genes encoding structural protein, calpainopathy was the initial reported kind of dystrophy predetermined by mutations within a gene encoding a proteolytic enzyme. (exposed-skin-care.net)
  • You can find over 440 noted mutations within the calpain 3 gene up to now, included in this 212 (50%) are missense mutations, a lot of which alter its catalytic activity (22). (exposed-skin-care.net)
  • It really is plausible which the subcellular localization of CAPN3 determines its particular mobile function, which mutations make a difference some of those features straight or indirectly (1). (exposed-skin-care.net)
  • Expression of the suppressor of cytokine signaling-1 (SOCS1) is inactivated in hematopoietic and solid cancers by promoter methylation, miRNA-mediated silencing, and mutations. (aacrjournals.org)
  • STAT3 binds to these sites via its SH2 (Src homology 2) domain, and is, in turn, tyrosine-phosphorylated by the receptor-associated JAKs. (nih.gov)
  • It then homodimerizes and translocates to the nucleus where it binds with high affinity to STAT-binding elements (SBE) in the promoters of various IL-10-responsive genes. (nih.gov)
  • We report that cten binds to another tumor suppressor, deleted in liver cancer 1 (DLC-1), and the SH2 domain of cten is responsible for the interaction. (rupress.org)
  • IRS then binds to the regulatory subunit of PI3K through its Src homology domains, and this interaction activates the catalytic unit. (diabetesjournals.org)
  • Unexpectedly, the interaction between DLC-1 and the cten SH2 domain is independent of tyrosine phosphorylation of DLC-1. (rupress.org)
  • By site-directed mutagenesis, we have identified several amino acid residues on cten and DLC-1 that are essential for this interaction. (rupress.org)
  • This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. (novusbio.com)
  • Structural determinants required for the interaction between Rho GTPase and the GTPase-activating domain of p190. (embl-heidelberg.de)
  • 1. Cheng, G. et al: Binding of Bruton's tyrosine kinase to Fyn, Lyn, or Hck through a Src homology 3 domain-mediated interaction. (signalchem.com)
  • Additionally, a protein-protein interaction (PPI) network was constructed to identify key genes involved in enhanced X-ray susceptibility of FaDu cells treated with RITA. (sagepub.com)
  • It is involved in flagellar movements and motility, and in cellular events that occur in the sperm head, that will lead to successful interaction with the egg and to fertilization [ 1 , 2 ]. (plos.org)
  • The tensin family member cten (C-terminal tensin like) is an Src homology 2 (SH2) and phosphotyrosine binding domain-containing focal adhesion molecule that may function as a tumor suppressor. (rupress.org)
  • The assay detected signal across at least 3 orders of magnitude of lysate input with a linear range spanning 1-2 orders and a low femtomole limit of detection for EGFR phosphotyrosine. (biomedcentral.com)
  • Noonan syndrome (NS) and NS with multiple lentigines (NSML) cognitive dysfunction are linked to SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) gain-of-function (GoF) and loss-of-function (LoF), respectively. (sdbonline.org)
  • East-Asian-type- cagA has a higher binding affinity for the Src homology-2 domain-containing phosphatase 2 (SHP2), resulting in a higher risk of peptic ulcer and/or gastric cancer than Western-type- cagA [ 4 - 7 ]. (biomedcentral.com)
  • BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors (1).Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. (signalchem.com)
  • Binding of IL-10 to the extracellular domain of IL-10R1 activates phosphorylation of the receptor-associated Janus tyrosine kinases, JAK1 and Tyk2. (nih.gov)
  • We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
  • SH2 profiling is a unique proteomic method in which interactions between an array of SH2 domains and protein samples are quantitatively analyzed, thereby defining the functional output of tyrosine phosphorylation. (biomedcentral.com)
  • Covalent modifications of Src mainly include phosphorylation and oxidization. (xcessbio.com)
  • Binding of insulin to its receptor activates the insulin receptor tyrosine kinase, resulting in autophosphorylation and phosphorylation of several substrates, including insulin receptor substrate (IRS)-1 through -4. (diabetesjournals.org)
  • PTPH1 overexpression in Jurkat T cells reduces indirectly the TCR-induced serine phosphorylation of Mek, Erk, Jnk and AP-1 leading to a decreased IL-2 gene activation [ 13 ]. (biomedcentral.com)
  • GRB2 overexpression led to increased formation of a complex between the guanine nucleotide-releasing factor Sos (the product of the mammalian homolog of son of sevenless gene) and GRB2. (nih.gov)
  • Src homology 2 (SH2)-containing 5'-inositol phosphatase localizes to podosomes, and the SH2 domain is implicated in the attenuation of bone resorption in osteoclasts. (nih.gov)
  • SRC homology 2 (SH2)-containing tyrosine phosphatase-2 (SHP-2) has a controversial effect on lymphocyte signaling. (biomedcentral.com)
  • Another negative regulator of hematopoietic cell development and function is SHP-1 (SRC homology 2 (SH2)-containing tyrosine phosphatase 1), that is mainly expressed in hematopoietic and lymphoid cells [ 8 ]. (biomedcentral.com)
  • c-Src tyrosine kinase consists of the N-terminal unique region, the Src homology 3 (SH3), SH2, linker, kinase domain, and the regulatory C-terminal tail. (intechopen.com)
  • The ~1.5 Mb candidate region was shown to contain five protein-coding genes. (biomedcentral.com)
  • The candidate interval was shown to exhibit an extraordinary level of alternate splicing - 19 transcripts were identified for the 5 protein coding genes, but it appears that a significant portion (14/19) of these alternate transcripts did not have an open reading frame, hence their functional role is questionable. (biomedcentral.com)
  • These calpains work as a heterodimer, comprising a big (80 kDa) and a little (28 kDa) subunit (1,4,5). (exposed-skin-care.net)
  • PI3K is composed of two subunits: the p85 regulatory subunit, containing two Src homology-2 domains, and the p110 catalytic subunit ( 2 - 4 ). (diabetesjournals.org)
  • In response to insulin stimulation, this complex bound to tyrosine-phosphorylated IRS-1 (insulin receptor substrate-1) and Shc. (nih.gov)
  • Calpain 3 provides 54 and 51% series homology towards the 80 kDa subunits of - and m-calpains, respectively, and stocks similar properties with one of these ubiquitously portrayed calpains such as for example Ca2+- reliant activation and maximal activity at natural pH (1). (exposed-skin-care.net)
  • The N-terminal A domain differs among the four subtypes of Ca 2+ channel β subunits (β 1 -β 4 ) primarily as the result of two duplications of an ancestral gene containing multiple alternatively spliced exons. (jneurosci.org)
  • this finding indicates that the gene for NFJ syndrome is not located in the epidermal differentiation complex, the type II keratin cluster, or the desmosomal cadherin cluster, respectively. (medscape.com)
  • The study identified three potential loci with a LOD score of ≥ 3, spanning across four candidate genes, namely SLC17A1 (rs1165196:pT269I), SLC17A3 (rs942379: p.S370S), TATDN2 (rs394558:p.V256I), and TMEM131L (rs6848033:p.R190R). (bvsalud.org)
  • Future studies may focus on replicating the findings in a larger T1D cohort and delineate the mechanistic details of the impact of these novel candidate genes on the pathophysiology of T1D. (bvsalud.org)
  • The underlying functions of the DEGs were predicted by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and BioCarta enrichment analysis. (sagepub.com)
  • Human G-CSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. (rndsystems.com)
  • It was later established that v-Akt is the oncogenic version of a cellular enzyme PKB/c-Akt, in which a truncated viral group specific antigen, gag, is fused in frame to the full length Akt−1 and is membrane bound whereas PKB/c-Akt is cytoplasmic. (justia.com)
  • Primary structure of mouse actin-related protein 1 (Arp1) and its tissue expression. (nih.gov)
  • Thus, the ability of IL-10 to inhibit gene expression in monocytes is associated with its ability to rapidly induce synthesis of SOCS-3. (nih.gov)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. (stowers.org)
  • We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. (stowers.org)
  • We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. (stowers.org)
  • Expression of each cluster's gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq. (stowers.org)
  • t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). (stowers.org)
  • Real-time PCR was used to study the expression pattern of formin genes. (biomedcentral.com)
  • Expression of forC, D, I and J increased during transition to multi-cellular stages, while the rest of genes displayed less marked developmental variations. (biomedcentral.com)
  • Various growth factors, cytokines, and prostaglandins upregulate HGF gene expression, including basic fibroblast growth factor, oncostatin M, hypoxia-inducible factor 1α and nuclear factor-κB (NF-κB) ( 9 ). (spandidos-publications.com)
  • By contrast, transforming growth factor (TGF)-β1 was demonstrated to markedly downregulate HGF gene expression ( 10 , 11 ). (spandidos-publications.com)
  • Although a high level of PDE10A gene expression is observed in the testis, information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. (plos.org)
  • CRKL is also required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression. (rockland.com)
  • Cytokine release, inflammatory pain and gene expression were investigated in challenged PTPH1-WT and KO mice. (biomedcentral.com)
  • We also identified several genes that do not appear to be protein coding, and generated 17 kb of novel transcript sequence data from re-sequencing 97 EST clones. (biomedcentral.com)
  • In this study, using RNA sequencing data from the HSCC cell line FaDu, we aimed to screen differentially expressed genes (DEGs) between 8 Gy X-ray-treated FaDu cells and 0 Gy X-ray-treated FaDu cells, as well as those between 8 Gy X-ray + RITA treated FaDu cells and 8 Gy X-ray treated FaDu cells. (sagepub.com)
  • Sequencing of Akt revealed a high degree of homology to PKA (˜75%) and PKC isozymes (˜50%), a fact which led to its renaming as PKB. (justia.com)
  • Since PKB activation is PI′-3-kinase dependent, the persistent activation of certain protein tyrosine kinases, such as IGF−1 receptor, EGF receptor, PDGF receptor, pp60c-Src, and the like, leads to the persistent activation of PKB which is indeed encountered in many tumors. (justia.com)
  • The novel gene MIA2 acts as a tumour suppressor in hepatocellular carcinoma. (nih.gov)
  • One of these genes, SOCS-3 (Suppressor of Cytokine Signaling-3) is a member of a newly identified family of genes that inhibit JAK/STAT-dependent signaling. (nih.gov)
  • Deletions in the gene coding for the tumor suppressor PTEN also induce the persistent activation of PKB/cAkt since it is the negative regulator of this enzyme. (justia.com)
  • The following Sh2b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • A nonsense mutation in a corresponding region of KRT5 has been found in Dowling-Degos disease and a missense mutation in the V1 domain of KRT5 has been described in patients with epidermolysis bullosa with mottled pigmentation. (medscape.com)
  • The patient had molecular analysis for KRT 14 exons 1, 4, and 6 and lacked a mutation. (medscape.com)
  • These observations support a mutation in a basal keratin gene as causing both blistering and pigmentary disorders. (medscape.com)
  • Mutation of the BTK gene and clinical feature of X-linked agammaglobulinemia in mainland China. (lu.se)
  • De novo mutation in the BTK gene of atypical X-linked agammaglobulinemia in a patient with recurrent pyoderma. (lu.se)
  • Activation of Src in human cancers employs a variety of mechanisms mainly including covalent modification, allosteric regulation, gene mutation. (xcessbio.com)
  • This gene is highly expressed in fetal brain and encodes a protein of relative molecular mass 91K, named oligophrenin-1, which contains a domain typical of a Rho-GTPase-activating protein (rhoGAP). (embl-heidelberg.de)
  • This gene encodes an intracellular tyrosine kinase expressed in T-cells. (wikipedia.org)
  • This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). (nih.gov)
  • Oligophrenin-1 encodes a rhoGAP protein involved in X-linked mental retardation. (embl-heidelberg.de)
  • It encodes the inactive pre-pro-HGF, a single chain of 728 amino acids (83 kDa), which includes a signal sequence ( 1 - 31 ), a heavy α chain (69 kDa), and a light β chain (34 kDa). (spandidos-publications.com)
  • Identification of novel Runx1 (AML1) translocation partner genes SH3D19, YTHDf2, and ZNF687 in acute myeloid leukemia. (atlasgeneticsoncology.org)
  • Thus, genetic studies from this Arab region are expected to lead to the identification of novel gene loci for T1D. (bvsalud.org)
  • Identification of differentially expressed genes associated with the enhancement of X-ray. (sagepub.com)
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution. (rndsystems.com)
  • These kinases then phosphorylate specific tyrosine residues (Y446 and Y496) on the intracellular domain of the IL-10R1 chain. (nih.gov)
  • The release of Ca 2+ from intracellular stores through the inositol 1,4,5-trisphosphate receptor (InsP 3 R) occurs in both excitable and nonexcitable cells. (jneurosci.org)
  • Furthermore, the calcium focus needed for activation is normally in the nanomolar range (weighed against micro and millimolar concentrations for calpain 1 and 2, correspondingly) (19). (exposed-skin-care.net)
  • This has prompted intensive investigations on γ-globin gene regulation, the outcome of which may provide insights for the therapeutic augmentation of HbF as treatment for the β-hemoglobinopathies. (biomedcentral.com)
  • In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. (novusbio.com)
  • c-Src (cellular Src), encoded by Src gene, is a non-receptor tyrosine kinase first isolated as the normal cellular homolog to the potent avian sarcoma viral transforming oncogene v-Src [ 4 ]. (intechopen.com)
  • The X protein of hepatitis B virus activates hepatoma cell proliferation through repressing melanoma inhibitory activity 2 gene. (nih.gov)
  • The major part of his production relates to variations ranging from protein engineering to effects and mechanisms of variations in protein structures, genes and diseases. (lu.se)
  • [ 4 ] The genetic defect appears to be a region of the gene encoding the KRT14 nonhelical head (E1/V1) domain located between the microsatellite markers D17S798 and D17S957, which are separated by approximately 26.97 cM. (medscape.com)
  • This domain is also known as the breakpoint cluster region-homology (BH) domain. (embl-heidelberg.de)
  • The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. (cancerindex.org)
  • however the existence of soluble G-CSF R in human serum has not been reported (1). (rndsystems.com)
  • Western Blot: PAG3 Antibody [NBP2-24614] - Analysis of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified this antibody. (novusbio.com)
  • Launch Calpain 3 (CAPN3) belongs to a Rabbit polyclonal to CDH1 family group of Ca2+- turned on natural cysteine proteinases which have been discovered in a multitude of microorganisms as disparate as human beings and worms (1,2). (exposed-skin-care.net)
  • In human beings, calpains are encoded by 15 genes with examined associates from the grouped family members getting the ubiquitously portrayed associates, calpains 1 and 2, referred to as - and m-calpain also, respectively. (exposed-skin-care.net)
  • The tyrosine kinase Src is activated in a large number of human malignancies and plays significant roles in the development of cancers. (xcessbio.com)
  • We have developed PLA-SH2, an alternative in-solution modular domain binding assay that takes advantage of Proximity Ligation Assay and real-time PCR. (biomedcentral.com)
  • We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
  • This feature along with short assay runtime makes this method a useful platform for the development of high throughput assays to determine modular domain-ligand interactions which could have wide-ranging applications in both basic and translational cancer research. (biomedcentral.com)
  • In an oligonucleotide-tagged multiplex (OTM) assay, a mixture of SH2 domains with domain-specific DNA tags are incubated with a sample spot allowing for competitive binding (single sample per well) [ 8 ]. (biomedcentral.com)