Gene reporter assay. Medical search. Frequent questions

TargetScan analysis predicted sphingosine 1-phosphate receptor-1 ( S1pr1 ) as a target gene of Mir155 , which was further confirmed by luciferase assay and Mir155 knockdown. (elifesciences.org)

In vitro transcriptional activation (TA) assays are based upon the production of a reporter gene product induced by a chemical, following binding of the chemical to a specific receptor and subsequent downstream transcriptional activation. (nih.gov)
Transactivation assays in yeast revealed the transcriptional activation potential of full-length OsHOX22 and OsHOX24 proteins. (frontiersin.org)
1) protocol for expression and purification of the active JH receptor complex, 2) new and efficient ligand-binding assay for JH and related compounds, and 3) cell-based reporter system to measure JH-receptor mediated transcriptional activation by JH and related compounds. (europa.eu)
6) A cell-based reporter system has been deviced to measure JH-dependent transcriptional activation of target genes. (europa.eu)

The assays measure the activities of downstream transcription factors, using either dual-luciferase or green fluorescent protein (GFP) reporter systems. (qiagen.com)
Gene ontology analysis of ZNF322A putative downstream genes elicited the contribution of ZNF322A in maintenance of CSC-like properties. (nature.com)
Online bioinformatics prediction software ( https://cn.string-db.org/ and https://www.genecards.org/ ) was used to determine potential downstream target genes of PTPN12. (degruyter.com)
EGFR and ERBB2 were selected as the downstream-regulation target genes. (degruyter.com)

To systematically characterize the drivers of cell identity among the unique cell types, we are using a novel massively parallel reporter assay to quantify enhancer activity. (nih.gov)
We introduce a new method, Reg-Seq, that links massively-parallel reporter assays with mass spectrometry to produce a base pair resolution dissection of more than 100 E. coli promoters in 12 growth conditions. (caltech.edu)
Massively parallel reporter assays of melanoma risk variants identify MX2 as a gene promoting melanoma. (cdc.gov)

We have investigated DA associated genotypes, using candidate gene studies, GWAS and Next Generation Sequencing (NGS) to identify gene variants associated with DA. (cdc.gov)
Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene. (cancerindex.org)
Address correspondence to Lisa rearrangements and rare variants that occur in the CYP2D6 gene. (cdc.gov)
For protein analyses, Dr. Sun uses EMSA, receptor binding and receptor signaling analysis luciferase binding assays to screen neurotransmitter receptor variants and analyze target membrane proteins. (nih.gov)
It's also not clear to me whether we have the right scalable technologies to engage in systematic interrogation of these gene variants, and/or the pathway to developing those scalable technologies. (nih.gov)
Genetic variants in PARP1 (rs3219090) and IRF4 (rs12203592) genes associated with melanoma susceptibility in a Spanish population. (cdc.gov)

Binding interactions between, hsa_circRNA_101996 and miR-143 and between, miR-143 and TET2 were detected by Dual-luciferase reporter assays. (jcancer.org)
dual-luciferase reporter assay was used to detect the targeting relationship between circ_0000419 and microRNA-224. (ijpsonline.com)
Studying the targeted regulatory relationship between miR-146b-3p and PTPN12 was based on dual luciferase reporter gene analysis. (degruyter.com)

Examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed [fr]. (wikipedia.org)

Electrophoretic mobility shift assays (EMSA) identified oligonucleotide-protein binding for risk and non-risk SNPs to nuclear extracts of A549, BEAS 2B, and IMR-90 lung cell lines. (cdc.gov)
Electrophoretic mobility shift assay established the binding of these homeobox proteins with specific DNA sequences, AH1 (CAAT(A/T)ATTG) and AH2 (CAAT(C/G)ATTG). (frontiersin.org)
Exposure of the cells to crocidolite asbestos caused a parallel increase in TNF production and NF-B activation, as analyzed by enzyme-linked immunosorbent assay and electrophoretic mobility shift assay. (cdc.gov)

Microarray analysis revealed differential expression of several stress-responsive genes in transgenic lines as compared to wild-type. (frontiersin.org)
Dr. Sun uses real-time PCR, reporter assays, nanostring, and microarray analysis to examine gene expression at the level of mRNA and protein. (nih.gov)
Global gene expression profiles in response to PPARα agonists were obtained by microarray analysis. (biomedcentral.com)

The human proteasome C3 subunit promoter contains elements homologous to the consensus NF- κ B-binding site ( Du et al, 2000 ), suggesting that NF- κ B may be involved in gene transcription. (nature.com)
Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. (nfkb-p65.org)
The particles contain a firefly luciferase gene driven by ARE located upstream of the minimal TATA promoter. (nfkb-p65.org)
The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. (nfkb-p65.org)
This method is an example of using cis-acting elements where the two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule. (wikipedia.org)
A recent study, evaluating LGI1 promoter, prodynorphin ( PDYN ), and GABA (B) receptor 1 ( GABBR1 ) genes in 104 sporadic IPEAF, did not show any statistically significant differences between patients and controls [ 15 ]. (hindawi.com)
The genetic construct, which is the gene of interest fused with the luciferase gene, is put next to its promoter sequence so that it will be expressed when the right stimulus is given. (beforeitsnews.com)
13. Optimization of a synthetic beta-catenin-dependent promoter for tumor-specific cancer gene therapy. (nih.gov)
14. Prostate-specific antigen promoter/enhancer driven gene therapy for prostate cancer: construction and testing of a tissue-specific adenovirus vector. (nih.gov)
15. Adenovirus mediated prostate specific enzyme prodrug gene therapy using prostate specific antigen promoter enhanced by the Cre-loxP system. (nih.gov)
20. Cre-loxP system as a versatile tool for conferring increased levels of tissue-specific gene expression from a weak promoter. (nih.gov)
Gene transfection assays using an expression plasmid containing a luciferase reporter gene and a TNF-derived NF-B gene promoter further indicated the dependence of NF-B activation on asbestos -induced gene expression. (cdc.gov)

When primary hepatocytes were exposed to these agents, transactivation of PPARα was elevated as measured by luciferase assay. (biomedcentral.com)

Bound proteins were purified by DNA affinity precipitation assays (DAPA) and eluted proteins analyzed by mass spectrometry (MS), demonstrating that rs14798008 bound H1 histones. (cdc.gov)

Compounds are evaluated in cell-based assays for inhibition of viral replication and reduction of virus yields, plaques, or cytopathic effect. (nih.gov)
However, those studies were confined to conventional hemagglutination-inhibition (HI) or microneutralization (MN) assays that are limited to propagation of the live lethal subtype H7N9 influenza virus in biosafety level 3 (BSL-3) laboratories. (cdc.gov)
Thus, Mir155 showed a catabolic effect on osteogenesis and bone mass phenotype via interaction with the S1pr1 gene, suggesting inhibition of Mir155 as a potential strategy for bone regeneration and bone defect healing. (elifesciences.org)

Many methods of transfection and transformation - two ways of expressing a foreign or modified gene in an organism - are effective in only a small percentage of a population subjected to the techniques. (wikipedia.org)
Dr. Sun has a wide range of expertise including Real-time PCR, genotyping, gene expression, oligonucleotide primer design, electrophoresis of DNA and proteins, and DNA sequencing as well as cell culture, receptor binding, and transfection techniques. (nih.gov)

Human IgG antibody Laboratories manufactures the luciferase reporter gene assay reagents distributed by Genprice. (nfkb-p65.org)

Transwell assays were used to analyze the knockdown effects of hsa_circRNA_101996, miR-143, and overexpression of TET2 on cell proliferation, migration, and invasion abilities. (jcancer.org)
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H -tetrazolium, clone formation, transwell migration, and transwell invasion assays were used to assess the proliferation, migration, and invasion ability of LSCC cells. (degruyter.com)

Cotransfection of a plasmid carrying a reporter gene and a reporter control siRNA is a commonly used control in high-throughput RNAi. (qiagen.com)
95% confluency and transiently transfected with a NF-κB-dependent luciferase reporter plasmid according to the manufacturer's instructions (Stratagene, La Jolla, USA). (medscape.com)

If you need to monitor cell signaling pathway activity, our gene reporter assays support your research by providing rapid, sensitive and quantitative assessment of signal transduction pathway activation. (qiagen.com)
Available as single pathway assays or as multi-pathway arrays, our gene reporter assays for signaling pathway analysis allow you to monitor an individual pathway or obtain a comprehensive view of multiple pathways involved in a biological process. (qiagen.com)

The AllStars Reporter Control should always result in high reporter-gene knockdown. (qiagen.com)

Glucocorticoids also induce gene transcription and protein synthesis of the NF- κ B inhibitor, I κ B, and inhibit the expression of cytokines ( Almawi and Melemedjian, 2002 ). (nature.com)
Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. (wikipedia.org)
and evidence that Met interacts with the circadian clock genes to induce exit from a reproductive diapause (Bajgar et al. (europa.eu)

Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. (nfkb-p65.org)
A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. (wikipedia.org)

Another approach is the indirect in vivo labeling of immune cells via PET reporter gene expression and involves the ex vivo genetic engineering of T lymphocytes with a reporter gene, reinfusion into the host, and the subsequent use of a PET probe specific for the reporter gene. (snmjournals.org)
In addition to their applications in basic research, luciferase reporter assays have also been used for therapeutic purposes, such as monitoring gene expression levels in patients with genetic diseases or identifying areas where drugs may bind to target proteins more efficiently. (beforeitsnews.com)
1. Pharmacological and genetic modulation of Wnt-targeted Cre-Lox-mediated gene expression in colorectal cancer cells. (nih.gov)
Enhancers are non-coding genetic elements that regulate transcription and are overwhelmingly responsible for cell type-specific gene expression. (nih.gov)
It had been previously shown that a genetic variant found within the IFITM-3 gene predisposes people to having a more severe disease following influenza infection. (nih.gov)
Most recently this same genetic variant has been associated with severe COVID-19 so this kind of just reinforces that this gene is particularly important. (nih.gov)

Luciferase activity was assayed using the Luciferase Assay Kit (Stratagene) according to the manufacturer's directions and detected with a Fluorostar Optima (BMG Labtech, Offenburg, Germany). (medscape.com)
Luciferase reporter assay exhibited that miR-146b-3p inhibited the luciferase activity of PTPN12 markedly. (degruyter.com)

Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. (cancerindex.org)
Indeed, overexpression of ZNF322A promoted self-renewal ability and increased stemness-related gene expressions in vitro and in vivo. (nature.com)
Overexpression of ZNF322A transcriptionally dysregulates genes in control of cell growth and motility, therefore contributes to lung tumorigenesis and poor prognosis. (nature.com)

Your second year builds on this knowledge and covers areas such as gene regulation, cell biology and metabolism. (kent.ac.uk)
5. The human programmed cell death-2 (PDCD2) gene is a target of BCL6 repression: implications for a role of BCL6 in the down-regulation of apoptosis. (nih.gov)
Many of these genes were found to be involved in transcriptional regulation and various metabolic pathways. (frontiersin.org)
However, up-regulation of PTPN12 and miR-146b-3p mimic suppressed ERBB2 protein expression but induced its gene expression. (degruyter.com)

SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. (mobitec.com)
This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. (wikipedia.org)
In the luciferase reporter assay, a gene that codes for the production of luciferase, an enzyme that makes light, is put into an organism. (beforeitsnews.com)
The enzyme luciferase is used in the luciferase reporter assay , which is a key part of bioluminescence imaging. (beforeitsnews.com)
An enzyme must oxidize its substrate, luciferin, for the luciferase reporter system to function. (beforeitsnews.com)
Our study shows, that certain phase I genes and their enzyme activities are increased by epigenetic modification in HepG2 cells with a concomitant reduction of EMT marker gene SNAIL. (cancerindex.org)

Therefore, various susceptibility genes and environmental factors are believed to be involved in the aetiology of TLE, which is considered to be a heterogeneous, polygenic, and complex disorder. (hindawi.com)

9. Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. (nih.gov)
This gene encodes a melanocortin receptor-interacting protein. (nih.gov)
Using this assay, the essential components of the JH receptor complex: the Met/Gce and Tai proteins were determined (Jindra et al. (europa.eu)

qRT-PCR and WB analyses were used to assess the mRNA and protein expression levels of target genes. (degruyter.com)
In this study, we examine the role of nuclear transcription factor-B (NF-B) and free oxygen radicals in asbestos -induced TNF gene and protein expression in lung macrophages. (cdc.gov)

In this study, we conducted genome-wide analyses including cell-based chromatin-immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq) analyses and in vitro oligo binding assay to identify ZNF322A transcriptional targets and to characterize ZNF322A DNA binding elements. (nature.com)

The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. (mobitec.com)
As a result, luciferase reporter assays are often used to test the activity of promoters and the way that reporter cells send and receive signals. (beforeitsnews.com)
PPARα/RXR heterodimer binds effectively to the peroxisome proliferator response elements (PPREs) located in promoters of various target genes and regulate the expression of genes involved in lipid metabolism and peroxisome proliferation [ 1 - 3 ]. (biomedcentral.com)

LPA receptors were originally classified in the Endothelial Differentiation Gene (EDG) family of receptors. (thermofisher.com)

In these cases, trans-acting elements, such as transcription factors are used to express the reporter gene. (wikipedia.org)

Proteolysis-inducing factor induced degradation of I- κ B α , nuclear accumulation of NF- κ B and an increase in luciferase reporter gene activity in myotubes containing wild-type, but not mutant, I- κ B α proteins. (nature.com)
The cell culture labo-ra-tory of VelaLabs supports Pharma and Biotech compa-nies with customized cell based assays to deter-mine bioac-tivity and prop-er-ties of biophar-ma-ceu-ti-cals, ther-a-peutic proteins, vaccines, etc. (vela-labs.at)

However, it is vital to continue replication studies and collaborative attempts to find significant results and thus determine which gene variant combination plays a definitive role in the aetiology of TLE. (hindawi.com)
let's make a knockout mouse, or let's make a gene variant mouse, let's study 30 cell lines with this mutation, or let's understand the impact of this mutation in an "X" system. (nih.gov)

Over-expression of OsHOX24 imparted higher sensitivity to stress hormone, ABA, and abiotic stresses in the transgenic Arabidopsis plants as revealed by various physiological and phenotypic assays. (frontiersin.org)

This well-established chromosome 10q24 locus contains leucine-rich, glioma-inactivated 1 ( LGI1 ) gene, which has a putative role in development [ 11 ]. (hindawi.com)

These results will yield the first functional enhancer map of human pancreas cells and advance our understanding of how these enhancers orchestrate the remarkable cell type-specific gene expression programs. (nih.gov)

11. Cell-specific Cre-mediated activation of the diphtheria toxin gene in pituitary tumor cells: potential for cytotoxic gene therapy. (nih.gov)
These results indicate that asbestos -induced TNF gene expression is mediated through a process that involves NF-B activation and free radical reactions. (cdc.gov)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. (labettor.com)
In this method, molecules that give off light, like luciferase reporter enzymes, are used to find target molecules or cells. (beforeitsnews.com)

Mutations in this gene have been associated with familial glucocorticoid deficiency type 2. (nih.gov)
Deletion of the p23 gene in the hsp82G170D mutant background had a greater effect on AhR signaling than the individual mutations, suggesting that these 2 mutations have separate actions on AhR signaling. (bioone.org)

During experimental setup using a reporter-gene construct, a reporter control siRNA can be used to determine optimal conditions. (qiagen.com)
When using a reporter-gene construct, a reporter control siRNA transfected in every experiment will indicate if conditions become suboptimal. (qiagen.com)
Reporter gene studies for nanoparticle mediated DNA and siRNA delivery. (cuni.cz)
Transfekce DNA a siRNA pomocí nanočástic: studium pomocí gene reporter assay. (cuni.cz)

Characterization of Gonadotropin-Releasing Hormone Analogs Based on a Sensitive Cellular Luciferase Reporter Gene Assay. (mpg.de)
While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. (cancerindex.org)

It is important to use a reporter gene that is not natively expressed in the cell or organism under study, since the expression of the reporter is being used as a marker for successful uptake of the gene of interest. (wikipedia.org)
Thus, a method for identifying those few successful gene uptake events is necessary. (wikipedia.org)

The now active HIF complexes regulate genes that are involved in hypoxic adaptation, including glucose metabolism, angiogenesis, and neurodegeneration. (jneurosci.org)

In this study, we carried out functional analysis of two candidate stress-responsive HD-ZIP I class homeobox genes from rice, OsHOX22 , and OsHOX24 . (frontiersin.org)

The Luciferase Reporter Gene Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (nfkb-p65.org)
In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. (wikipedia.org)
Reporter genes are often used as an indication of whether a certain gene has been taken up by or expressed in the cell or organism population. (wikipedia.org)
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. (wikipedia.org)
Reporter genes can produce a protein that has little obvious or immediate effect on the cell culture or organism. (wikipedia.org)
Finally, recommendations are provided to guide the experimental design for germ cell mutagenicity data for regulatory submission, as well as other possible approaches to increase the reliability of the TGR assay. (nih.gov)
The compounds are tested with cell-based assays, and the resulting data are used to decide whether further studies in animal models of infection are warranted. (nih.gov)
Researchers can study dynamic processes like cell proliferation, apoptosis, and signal transduction pathways by using luciferase reporter assays instead of killing animals or other organisms. (beforeitsnews.com)
In this technique, a gene of interest is fused to a luciferase gene and inserted into a cell. (beforeitsnews.com)
GeneBLAzer™ EDG7-NFAT- bla HEK293T cells contain the human Endothelial Differentiation Gene (EDG7) stably integrated into the CellSensor™ NFAT- bla HEK293T cell line. (thermofisher.com)
Cell activity was detected by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. (ijpsonline.com)
Some of the gene changes were associated with production of reactive oxygen species, cell proliferation of peroxisomes, and hepatic disorders. (biomedcentral.com)

The ARE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. (nfkb-p65.org)
Only those cells that have successfully taken up the construct containing the CAT gene will survive and multiply under these conditions. (wikipedia.org)
Bioluminescence imaging is based on luciferase reporter assays, which have been used for decades to watch what is happening inside cells in real time. (beforeitsnews.com)
After expressing the gene construct, one can measure luminescence from the cells using a luminometer or other instrumentation. (beforeitsnews.com)
Also, luciferase reporter assays don't hurt the cells or tissues and don't need to label or stain them. (beforeitsnews.com)
7. Butyrate induced changes in Wnt-signaling specific gene expression in colorectal cancer cells. (nih.gov)
A luciferase reporter assay, in A549 cells, identified allele-dependent mRNA transcription. (cdc.gov)
We have cloned thousands of candidate enhancers into lentiviral reporter libraries and are assessing activity of these elements in primary pancreas cells obtained from healthy donors. (nih.gov)
We simply wanted to do those first set of experiments to better understand whether this is an antiviral gene that is active against Sars-CoV-2, the cause of COVID-19, and what type of impact it has on cells that are physiologically relevant for infections in our bodies. (nih.gov)
K1538) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element. (thermofisher.com)
In addition, GeneBLAzer™ EDG7-NFAT- bla HEK293T cells have been tested for assay performance under variable conditions. (thermofisher.com)
Moreover, PTPN12 serves as a tumor suppressor gene through regulating the proliferation, migration, and invasion of LSCC cells. (degruyter.com)

Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida. (nih.gov)

However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. (cdc.gov)

In addition, peroxisome proliferation and gene expression regulated by PPARα were not remarkably altered. (biomedcentral.com)

The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Placental alkaline phosphatase standard, "mix and read" assay protocol that is compatible with high throughput screening. (mobitec.com)
Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. (cancerindex.org)

We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls. (cdc.gov)

Anatomy23

Organisms13

Diseases3

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes80

Disciplines and Occupations1

Information Science3

Confirmed by luciferase assay1

Transcriptional activation4

Downstream4

Massively parallel reporter3

Variants6

Dual-luciferase reporter3

Green fluores1

Electrophoretic3

Microarray analysis3

Promoter12

Transactivation1

Mass spectrometry1

Inhibition3

Transfection2

Reagents1

Transwell2

Plasmid2

Signaling pathway2

Knockdown1

Induce3

Beta-galactosidase2

Genetic6

Luciferase activity2

Overexpression3

Regulation4

Enzyme6

Susceptibility1

Receptor3

Protein expression2

Vitro1

Promoters3

RECEPTORS1

Transcription factors1

Proteins2

Variant2

Transgenic1

Putative1

Enhancer1

Activation2

Target2

Mutations2

SiRNA4

Cellular2

Uptake2

Regulate1

Functional1

Cell12

Cells12

Activity1

Laboratory1

Proliferation1

Placental2

Assessment1