Gene regulates bovine skeletal muscle satellite cell proliferation and myogenic differentiation skeletal muscle proliferation and differentiation. Medical search. Frequent questions

Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Satellite cells and skeletal muscle regeneration, Compr. (copernicus.org)
In the context of muscle development and regeneration, hnRNPs are involved in a wide range of regulatory mechanisms, including alternative splicing, transcription regulation, miRNA regulation, and mRNA stability regulation. (bvsalud.org)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
Therefore, promotion of myoblast proliferation and differentiation and induction of myotube hypertrophy should be beneficial for muscle regeneration and control of muscle mass. (kosfaj.org)

Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
To understand the function of SFRP1 and miRNA-1/206 in swine myogenesis, we first predicted the targets of miRNA-1/206 with the TargetScan and PicTar programs, and analyzed the molecular characterization of the porcine SFRP1 gene. (biomedcentral.com)
Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)

Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells. (biomedcentral.com)

When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
These monocyte myoblasts proliferate and differentiate, following which, they fuse with existing muscle fibers to form multinucleated myotubes and myofibers. (kosfaj.org)

Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)

To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
Comparison of transcriptome of skeletal muscles and other tissues between phenotypically different pig breeds has been proposed to improve the understanding of the genetic mechanisms underlying differences in growth and meat quality [ 10 ]. (biomedcentral.com)
The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
We observed that, as skeletal muscle progressed from perinatal to postnatal and adult developmental stages, MSY3 protein became gradually dephosphorylated and accumulated in the nucleus. (biomedcentral.com)
Furthermore, forced expression of Akt in adult skeletal muscle induced MSY3 phosphorylation and myogenin derepression. (biomedcentral.com)
Given the significance of myogenin regulation in adult muscle, the Akt/MSY3/myogenin regulatory circuit is a potential therapeutic target to counteract muscle degenerative disease. (biomedcentral.com)
Circular transcripts of the testis-determining gene Sry in adult mouse testis, Cell, 73, 1019-1030, https://doi.org/10.1016/0092-8674(93)90279-y, 1993. (copernicus.org)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)

Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
We showed previously that cytoplasmic release of mtDNA activates the cGAS STING TBK1 pathway resulting in interferon-stimulated gene (ISG) expression that promotes antiviral immunity4. (regenerativemedicine.net)

Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, Ban S. "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. (sciepub.com)
Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)

Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)
Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. (kosfaj.org)

Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)

Cell Metabolism 2013;17(2):210-24. (scielo.br)
In this report, we provide an overview of our current understanding of how hnRNPs regulate RNA metabolism and emphasize the significance of the key members of the hnRNP family in muscle development. (bvsalud.org)
We observed alteration in biological functions related to extracellular matrix function and organization, cellular adhesion, muscle growth, lipid metabolism and proteolysis. (biomedcentral.com)
Genes related to lipid metabolism and proteolysis were found among those upregulated in IB. (biomedcentral.com)
Muscle atrophy with aging is accompanied by muscle damage caused by oxidative damage and malnutrition caused by lack of muscle metabolism. (kosfaj.org)

Basic helix-loop-helix (bHLH) myogenic regulatory factors coordinate the correct function and organization of many skeletal muscle functions. (biomedcentral.com)
Of particular importance has been the identification of two families of transcription factors: the myogenic regulatory factors (MRFs) and the myocyte enhancer factors (MEFs). (biologists.com)
The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. (biomedcentral.com)

This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
The KEGG pathway enrichment analysis showed that the down-regulated DEmRNAs were mainly enriched in adrenergic signaling, axon guidance, calcium signaling pathway, etc. (bvsalud.org)
The up-regulated DEmRNAs were enriched in the adhesion function, chemokine signaling pathway, apoptosis, etc. (bvsalud.org)
The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
The SFRPs inhibit Wnt receptor binding to down-regulate pathway signaling during development [ 8 ]. (biomedcentral.com)

LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)

Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)
Piglets (14 from each genetic type) were slaughtered at weaning (28 days) and longissimus dorsi was sampled for composition and gene expression studies. (biomedcentral.com)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
Possible MSY3 mechanisms of action have been studied in cell culture studies. (biomedcentral.com)
However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
then we review the studies on the molecular mechanisms of LPLI-induced proliferation since January 1999, which will serve as a reference for the researchers in this field. (biomedcentral.com)

This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
Musgrove E, Seaman M, Hedley D. "Relationship between cytoplasmic pH and proliferation during exponential growth and cellular quiescence. (sciepub.com)

The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)

Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
In the presence of antler extracts, the length and thickness of myotubes and myogenin differentiation 1 (MyoD1) and myogenic factor 5 (Myf5) gene expression were increased compared to those in the control group (CON). (kosfaj.org)

Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)

While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)

We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)

Cell Stem Cell 2013;13(5):590-601. (scielo.br)
Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
Although adipocyte differentiation starts early in fetal stages, the increase in adipose cell number and size is maintained along early postnatal growth and later development [ 6 ]. (biomedcentral.com)

Yamada K. "Biological effects of low power laser irradiation on clonal osteoblastic cells (MC3T3-E1). (sciepub.com)

Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)

Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
By utilizing gene diversity in the major histocompatibility complex gene group, it was found that there were several single-nucleotide polymorphisms in the B Locus Beta 2 gene significantly associated with immunoglobulin Y concentrations in IPB-D2 chickens. (copernicus.org)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. (kosfaj.org)

The functional enrichment analysis indicate that the down-regulated mRNAs are mainly involved in cardiac muscle cell contraction, potassium channel activity, etc. and the up-regulated mRNAs are mainly involved in T cell differentiation and mononuclear cell migration, etc. (bvsalud.org)

Expression of muscle growth related genes was quantified with qPCR. (nature.com)
Transcript differences were validated for a subset of DE genes by qPCR. (biomedcentral.com)

Muscle growth of low birth weight (LBW) piglets may be improved with adapted nutrition. (nature.com)
More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)
Our previous studies showed that Gln had the potential to increase muscle fiber size in piglets 16 , but the mechanism was not clear. (nature.com)

The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. (kosfaj.org)
In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. (kosfaj.org)

The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
These proteins play a crucial role in various biological processes by regulating RNA splicing, transcription, and translation through their binding to RNA. (bvsalud.org)

The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

Knocking down Akt expression increased the amount of dephosphorylated MSY3 and reduced myogenin expression and muscle differentiation. (biomedcentral.com)
Maternal obesity alters circRNA expression and the potential role of mmu_circRNA_0000660 via sponging miR_693 in offspring liver at weaning age, Gene, 731, 144354, https://doi.org/10.1016/j.gene.2020.144354, 2020. (copernicus.org)
In addition, increased expression of PVT1, 1700071M16Rik, Tox and Themis2 may be considered as potential diagnostic gene biomarkers in AR. (bvsalud.org)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
Correlation among the expression of these RF and DE genes show relevant differences between genetic types. (biomedcentral.com)
Here, we find that persistent mtDNA stress is not associated with basally activated NF-κB signalling or interferon gene expression typical of an acute antiviral response. (regenerativemedicine.net)

Loin muscle chemical composition showed significant differences between genetic types in intramuscular fat content (6.1% vs. 4.3% in IB and DUxIB animals, respectively, P = 0.009) and in saturated ( P = 0.019) and monounsaturated fatty acid proportions ( P = 0.044). (biomedcentral.com)
Within skeletal muscle, lipids are stored as droplets both in myofiber cytoplasm and in adipocytes (interspersed between fiber fasciculi) with IMF deposition being highly dependent on the number of intramuscular adipocytes [ 5 ]. (biomedcentral.com)

Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)

These results support the hypothesis that MSY3 phosphorylation by Akt interferes with MSY3 repression of myogenin circuit activity during muscle development. (biomedcentral.com)
We have previously shown that MSY3/Csda binds a highly conserved cis-acting element located upstream of the myogenin promoter (myogHCE) and regulates the postnatal down-regulation of myogenin [ 16 ]. (biomedcentral.com)

Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)

Other two pathways, ΔΨm/ATP/cAMP/JNK/AP-1 and ROS/Src, are also involved in LPLI-induced proliferation. (biomedcentral.com)

Differentially expressed ceRNA involved in programmed cell death were further verified with qRT-PCR testing. (bvsalud.org)

Yamaguchi DT, Huang JT, Ma D. "Regulation of gap junction intracellular communication by pH in MC3T3-E1 osteoblastic cells. (sciepub.com)

Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)

The formation of skeletal muscle:from somite to limb. (scielo.br)

Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)

Hepatocyte growth factor receptor (c-Met), a member of tyrosine protein kinase receptors (TPKR), is phosphorylated during LPLI-induced proliferation, but tumor necrosis factor alpha (TNF-alpha) receptor has not been affected. (biomedcentral.com)

Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)

We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)

These findings increase understanding of the biological functions and the regulation of the SFRP1 gene in mammals. (biomedcentral.com)

Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)

Anatomy18

Organisms5

Diseases7

Chemicals and Drugs18

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes19

Disciplines and Occupations1

Information Science2

Regeneration15

Myogenesis6

Porcine4

Myofibers4

Progenitor cells2

Tissues5

Adult8

Promotes3

Osteoblast-like cells3

C2C123

Vitro4

Embryonic2

Metabolism5

Regulatory3

Pathway5

Progression1

Longissimus dorsi2

Mechanisms4

Cellular3

Rudnicki1

Myotubes2

Sarcopenia1

Ablation2

Model of Duchenne muscular1

Stem Cell3

Adipose2

Clonal1

Regenerative capacity1

Significantly5

Cardiac1

QPCR2

Piglets4

Atrophy2

Transcription2

Vivo3

Expression7

Intramuscular2

Extracellular3

Myogenin2

Power laser irradiation1

Pathways1

Differentially1

Intracellular1

Development1

Limb1

Characterization1

Receptor1

Tissue1

Functional2

Biological1

Molecular1