Gene receptor erbb 2. Medical search. Frequent questions

The authors studied DNA amplification of the HER-2/neu gene on 4-microm sections obtained from 62 formalin-fixed, paraffin-embedded PCs by fluorescence in situ hybridization (FISH). (nih.gov)
Amplification of HER-2/neu correlated with Gleason score (P = .0001). (nih.gov)
Nondiploid tumors had a significantly greater rate of HER-2/neu amplification compared with diploid tumors (P = .0003). (nih.gov)
There was no correlation of HER-2/neu amplification by FISH with patient age or preoperative serum PSA levels. (nih.gov)
The authors conclude that HER-2/neu gene amplification status can be determined by FISH on archival prostate cancer specimens, significantly correlates with high tumor grade and nondiploid DNA content, and is more frequently encountered in tumors with advanced pathological stage. (nih.gov)
ERBB2 gene amplification detected by fluorescent differential polymerase chain reaction in paraffin-embedded breast carcinoma tissues. (thieme-connect.de)
HER-2/neu amplification predicts poor survival in node-positive breast cancer. (thieme-connect.de)
14 King C R, Kraus M H, Aaronson S A. Amplification of a novel v-erbB-related gene in a human mammary carcinoma. (thieme-connect.de)
Amplification of the HER-2 gene with overexpression of the protein occurs in 20-30% of breast cancers, and tumor progression in mammary cancer is associated with elevated levels of tyrosine-phosphorylated neu and erbB-3. (ihcworld.com)
One type of breast cancer is identified by the human epidermal growth factor receptor 2 (HER2) gene amplification. (carcinogenesis.com)
Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. (activemotif.com)
Positive staining, which is known to correlate with gene amplification, was associated with earlier relapse, shorter postrelapse survival, and shorter overall survival. (ox.ac.uk)
Gene amplification and overexpression of the proteins in breast malignancies (BCs) anticipate poor disease final result due to raised metastatic potentials4,5,6,7. (president2010.info)
C-myc amplification was significantly correlated to a high S-phase fraction and to amplification of the c-erbB-2 proto-oncogene. (lu.se)
Weak relationships were found between c-myc amplification and the presence of lymph-node metastasis, advanced stage, DNA non-diploidy and premenopausal status, but not tumor size, estrogen receptor or progesterone receptor status, or int-2 amplification. (lu.se)
C-myc amplification, and especially a high gene copy number (greater than 5 copies), was significantly related to early recurrence and death in breast cancer, a relationship seen in both the lymph-node-negative and node-positive subcategories. (lu.se)
Gene amplification, on the other hand, is relatively infrequent and occurs mostly at low copy number, implying that tumors are heterogeneous with respect to cell clones harboring c-myc amplification. (lu.se)
Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. (arigobio.com)
The Human HER2 Gene Copy Number Variation Detection Assay allows for the accurate assessment of HER2 gene amplification or deletion in human DNA samples. (rainsureglobal.com)
Loss of heterozigosity (LOH) EGFR overexpression/amplification and cyclooxygenase-2 (COX2) dysregulation in pre-malignant lesions have been associated with … The EGFR has been implicated in head and neck squamous cell carcinoma (HNSCC) carcinogenesis. (ecolowood.com)
Subsequent drive gene analysis with the method of amplification refractory mutation system (ARMS) using a collected tissue sample suggested a deletion of EGFR exon 19 without T790 M mutation. (biomedcentral.com)
In 20-30% of breast carcinomas, Her-2/ neu status is altered, and this is manifested either as amplification of the gene or overexpression of the protein product [ 3 ]. (biomedcentral.com)
A subset of patients with tumors having 2+ IHC results were found to show no response to the drug, whereas all those having gene amplification responded favorably. (biomedcentral.com)
Nevertheless, a negative (0 or 1+) or a 3+ Her-2/ neu IHC correlates well with a negative or positive Her-2/ neu gene amplification, respectively. (biomedcentral.com)
Her-2/ neu gene amplification is primarily detected by in situ hybridization and uses fluorescence (FISH) to detect the signals. (biomedcentral.com)
CISH is potentially able to detect Her-2/ neu gene amplification and to minimize, if not eliminate, the false positive fraction with the IHC procedure. (biomedcentral.com)
Amplification, also known as the over-expression of the ERBB2 gene, occurs in approximately 15-30% of breast cancers . (iiab.me)
Amplification and overexpression of the neu gene have been found in several human adenocarcinomas. (prinsesmaximacentrum.nl)
Genetic changes include mutations, gene silencing through epigenetics, gene amplification or deletion and whole chromosome gains or losses. (bmj.com)
Alterations in receptor tyrosine kinases (TKs), such as the epidermal growth factor receptor (EGFR) and insulin-like growth factor receptor 1, include overexpression, amplification or mutations. (bmj.com)
Human epidermal receptor growth factor 2 ( HER2 , ERBB2 ) gene amplification occurs in about 15-20% of invasive breast cancers and is associated with biologically aggressive disease and poor overall survival in the absence of systemic therapy. (medscape.com)
In fact, it not only yielded, in combination with chemotherapy, a significant improvement in progression-free and overall survivals, [ 2 ] but also allowed the evolution of the other HER2-targeted therapies, which currently represent the mainstay of treatment for locally advanced and metastatic breast cancer (mBC) with an HER2 amplification. (medscape.com)

In TGF-beta-treated MCF10A mammary epithelial cells overexpressing HER2 and by chromatin immunoprecipitation, we identified novel Smad targets including protein tyrosine phosphatase receptor type kappa (PTPRK). (nih.gov)
Endogenous RPTPkappa associated with EGF receptor and HER2, resulting in suppression of basal and ErbB ligand-induced proliferation and receptor phosphorylation. (nih.gov)
With the addition of trastuzumab, a monoclonal antibody targeting the human epidermal growth factor receptor-2 (HER2), improvements in overall survival have been observed among patients with advanced HER2-positive disease. (carcinogenesis.com)
HER2 is a member of the cErbB family of receptor tyrosine kinases, and is responsible for HER2-mediated signal transduction in the cytoplasm. (carcinogenesis.com)
This membrane deformation will not need receptor signalling actions, but outcomes from the high degrees of HER2 in the cell surface area. (president2010.info)
HER2 is certainly a CAY10471 Racemate known person in the ErbB/HER receptor tyrosine kinase family members1,2,3. (president2010.info)
HER2 BC cells using a 3+ immunohistochemistry (IHC) rating display high basal degrees of receptor kinase activity and phosphorylation, and these induce CAY10471 Racemate constitutive activation from the mitogen-activated proteins phosphatidylinositol and kinase 3-kinase/Akt pathways8. (president2010.info)
These features of HER2 overexpression had been acquired from several ensemble experiments, where receptor actions are averaged more than an extremely large numbers of cells typically. (president2010.info)
A lot of area points of specific HER2s had been obtained by monitoring one receptors (for 100?s in a 10.72?Hz acquisition price) labelled with anti-HER2 Fab:QD (H2Fab:QD) conjugates using total inner reflection fluorescence microscopy (TIRFM)13. (president2010.info)
We initial likened the receptor distribution patterns inside the same cell type where HER2 appearance levels had been artificially changed. (president2010.info)
The HER Alosetron family is comprised of four unique receptors: EGFR (also known as HER1 or ErbB-1) HER2 (ErbB-2 Neu) HER3 (ErbB-3) and HER4 (ErbB-4). (ecolowood.com)
HER2 does not have any identified ligand an undeniable fact explained from the structure from the extracellular area from the receptor which is already in an activated conformation and does not allow ligand docking. (ecolowood.com)
It is also frequently called HER2 (from human epidermal growth factor receptor 2 ) or HER2/neu . (iiab.me)
HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. (iiab.me)
HER2 is so named because it has a similar structure to human epidermal growth factor receptor, or HER1. (iiab.me)
Molecular cloning of the gene showed that HER2, Neu, and ErbB-2 are all encoded by the same orthologs. (iiab.me)
[10] [11] HER2 can heterodimerise with any of the other three receptors and is considered to be the preferred dimerisation partner of the other ErbB receptors. (iiab.me)
HER2 is colocalised and most of the time, coamplified with the gene GRB7, which is a proto-oncogene associated with breast, testicular germ cell, gastric, and esophageal tumours. (iiab.me)
The AP-2 genes are expressed in many human breast cancer cell lines, and critical AP-2-binding sites are present in both the ERBB-2 (HER2/neu) and estrogen receptor promoters. (johnshopkins.edu)
Last, continuing HER2 blockade with trastuzumab in combination with several chemotherapeutic agents is preferable for subsequent lines of therapy, and the combinations of lapatinib [a dual kinase inhibitor that targets the intracellular domain of HER2 and epidermal growth factor receptor (EGFR)] with trastuzumab or capecitabine are reasonable treatment options for some patients. (medscape.com)

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. (thermofisher.com)
EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. (thermofisher.com)
EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. (thermofisher.com)
Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. (thermofisher.com)
Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. (thermofisher.com)
Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. (thermofisher.com)
c- erb B-2 (HER-2/ neu ) is a 185 kDa receptor tyrosine kinase belonging to the epidermal growth factor receptor family, which also includes EGFR (HER-1), c- erb B-3 (HER-3), and c- erb B-4 (HER-4). (ihcworld.com)
The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). (activemotif.com)
In our prior research of epidermal development aspect receptor (EGFR) dimerization dynamics at a single-molecule level, we demonstrated that receptor activation by ligand binding is certainly spatially asymmetric on cells overexpressing EGFR (ref. 13). (president2010.info)
sub:provenance { sub:assertion dcterms:description "[C-erbB-2 and epidermal growth factor receptor (EGFR) genes were independently shown to be associated with breast cancer progression. (vu.nl)
C-erbB-2/HER-2 is a member of the EGFR family. (neobiotechnologies.com)
The epidermal growth factor receptor (EGFR) continues to be implicated in head and neck squamous cell carcinoma (HNSCC) carcinogenesis. (ecolowood.com)
The EGFR is definitely a 170-kd protein encoded by a 110-kb-long gene localized in the short arm of chromosome 7. (ecolowood.com)
They may be created as transmembrane precursors and could become subdivided into three organizations according with their affinity for just one or even more HER receptors: the 1st group includes ligands that bind specifically to EGFR (e.g. (ecolowood.com)
Once activated these pathways contribute to the development of a malignant cellular phenotype including resistance to apoptosis increased proliferation invasion metastasis and stimulation of angiogenesis (Figure 1).1 Figure 1 Epidermal growth factor receptor (EGFR) pathway activation during HNSCC carcinogenic process. (ecolowood.com)
Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) have been emerged as the standard selection in non-small cell lung cancer (NSCLC) patients with EGFR sensitive mutations. (biomedcentral.com)
Owing to the well-established beneficial outcomes of clinical trials, epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs), have been approved as the standard regimens in patients with non-small cell lung cancer (NSCLC) harboring EGFR sensitive mutations [ 1 ]. (biomedcentral.com)
ErbB-2 was named for its similarity to ErbB (avian erythroblastosis oncogene B), the oncogene later found to code for EGFR. (iiab.me)
The epidermal growth factor receptor (EGFR) tyrosine kinase (TK) plays an important role in the pathogenesis of NSCLC. (bmj.com)

These in vivo data suggest that the Gq-coupled 5-HT(2B)R uses the signaling pathway of the tyrosine kinase receptor ErbB-2 for cardiac differentiation. (hal.science)

ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. (rndsystems.com)
Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. (rndsystems.com)
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-2 Mouse Monoclonal Antibody (ERBB2/3078). (neobiotechnologies.com)
SDS-PAGE Analysis Purified HER-2 Mouse Monoclonal Antibody (ERBB2/3078). (neobiotechnologies.com)
Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/3078). (neobiotechnologies.com)
Receptor tyrosine-protein kinase erbB-2 , also known as CD340 (cluster of differentiation 340), proto-oncogene Neu , Erbb2 (rodent), or ERBB2 (human), is a protein that in humans is encoded by the ERBB2 gene. (iiab.me)

In addition to plexins, various other proteins have been implicated either as receptors for semaphorins or as components of the semaphorin receptor complex ( Pasterkamp and Kolodkin, 2003 ). (rupress.org)
Here, we show that the dual inhibitor of ErbB receptors, lapatinib, enhances cytostatic and induces cytotoxic effects of GTC in two bladder cancer cell lines which differ with regard to expression levels of proteins taking part in the ErbB pathway. (edu.kz)
ABA is a stress hormone perceived by the soluble receptor PYR1 (encoded by Pyrabactin resistance 1 ) and its related PYR1- like and R egulatory C omponent of ABA R ecepto r proteins ( PYL/RCAR , PYLs for simplicity) 18 . (nature.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
Previous in vitro studies have also indicated that the AP-2 family of proteins may be involved in the etiology of human breast cancer. (johnshopkins.edu)
There was also a significant correlation between the presence of the AP-2α protein and estrogen receptor expression (P=0.018) and between specimens containing both AP-2α/AP-2γ proteins and ERBB-2 expression (P=0.003). (johnshopkins.edu)
Thus, AP-2 proteins may directly regulate the transcription of this growth factor receptor. (johnshopkins.edu)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

The results were validated both by gene expression analysis and immunohistochemistry study in an independent 55 clinical samples from Fudan University Shanghai Cancer Center. (cancerindex.org)
Results of estrogen receptor and c-erbB-2 status using immunohistochemistry were documented. (ispub.com)
Chromogenic in situ hybridization (CISH) is emerging as a practical, cost-effective, and valid alternative to fluorescent in situ hybridization in testing for gene alteration, especially in centers primarily working with immunohistochemistry (IHC). (biomedcentral.com)

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. (rndsystems.com)
Novel therapeutic agents, in particular those that specifically target members of the human epidermal growth factor receptor (HER (ErbB1)) pathway, have shown encouraging therapeutic efficacy. (bmj.com)

The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1 (NRG1), another member of the neuregulin family of ligands. (wikipedia.org)
As ErbB-family ligands and receptors are highly expressed within the stem-cell niche 7 , we hypothesize that strong endogenous regulators must control the pathway in the stem-cell compartment. (nature.com)
Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. (activemotif.com)
Its structure consists of an extracellular ligand-binding website a Alosetron single transmembrane hydrophobic helix and a cytoplasmic carboxy-terminal website to which tyrosine kinase activity is definitely limited.1 HER receptors are usually located in the basolateral membrane of the epithelial cells where they can interact with their ligands present in the stroma thus mediating signaling between the epithelium and the extra-cellular matrix. (ecolowood.com)
The ligands to HER receptors (also known as epidermal growth factor [EGF] family of growth factors) are characterized by the presence of an EGF-like website (composed of three disulfide-bonded intramolecular organizations which confer binding specificity) and extra structural motifs (such as for example immunoglobulin-like domains heparin-binding sites and glycosilation sites). (ecolowood.com)
The final cleavage is performed by -secretase, whose substrates include all four Notch receptors and their ligands as well as -amyloid precursor protein, E-cadherin, CD44, ErbB-4, and ephrin-B1 [2-8]. (scienceexhibitions.org)

Nodal positivity correlated with histological grade and tumour size, but not with estrogen receptor or c-erbB-2 status. (ispub.com)
Chi square was used to test the association between ethnic group, tumour size (categorized as ≤2 cm, >2 cm and ≤5 cm, and >5 cm), histological grade, estrogen receptor (ER) status, c-erbB-2 status and lymph node metastases. (ispub.com)
Lymph node, epidermal growth factor receptor, and estrogen receptor status, tumor size, and histological grade also had prognostic significance but, applying multivariate analysis, only lymph node status was a more important predictor of relapse-free and overall survival than staining for the oncoprotein. (ox.ac.uk)
Positive staining was correlated with negative estrogen receptor status and high histological grade, but there was no association with either lymph node or epidermal growth factor receptor status or tumor size. (ox.ac.uk)
Your Milliliters classifier was validated inside self-sufficient training (n = 40) along with affirmation (n = 109) cohorts discovering MSR, PSA, and also ISP to become associated with growth stage, lymph node reputation, Nottingham quality, stromal difference (SD), cancer dimension, excess estrogen receptor (Im), progesterone receptor (Public relations), and also receptor tyrosine-protein kinase erbB-2 (HER-2). (hif-signal.com)

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. (cancerindex.org)
Here, we show that plexin-B family members stably associate with the receptor tyrosine kinase ErbB-2. (rupress.org)
Binding of Sema4D to plexin-B1 stimulates the intrinsic tyrosine kinase activity of ErbB-2, resulting in the phosphorylation of both plexin-B1 and ErbB-2. (rupress.org)
A cell surface protein-tyrosine kinase receptor that is overexpressed in a variety of ADENOCARCINOMAS. (bvsalud.org)
Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. (rndsystems.com)
However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. (arigobio.com)
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. (arigobio.com)
Following dimerization there is increased intracellular kinase activity of the receptor through a proximity effect resulting in phosphorylation of critical tyrosine residues on the cytoplasmic domain which then triggers the signal transduction cascade. (ecolowood.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
Inactivation of the 5-HT(2B) receptor (5-HT(2B)R) gene leads to embryonic and neonatal death due to the following defects in the heart: (a) 5-HT(2B)R mutant embryos exhibit a lack of trabeculae in the heart and a reduction in the expression levels of a tyrosine kinase receptor, called ErbB-2, leading to mid-gestation lethality. (hal.science)
lacks kinase domain), and erbB-4. (iiab.me)

Expression analyses of epidermal growth factor receptor and HER-2/neu: no advantage of prediction of recurrence or survival in breast cancer patients. (thieme-connect.de)
MA5-14485 targets Epidermal Growth Factor Receptor in IHC (P) applications and shows reactivity with Human samples. (thermofisher.com)
Importance of epidermal growth factor receptor signaling in establishment of adenomas and maintenance of carcinomas during intestinal tumorigenesis. (nature.com)
ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. (rndsystems.com)
Violin plots show distribution of expression levels for epidermal growth factor receptor 2 (SMED30029442) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
Expression of epidermal growth factor receptor 2 (SMED30029442) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)
Violin plots show distribution of expression levels for epidermal growth factor receptor 2 (SMED30029442) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells. (stowers.org)
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. (arigobio.com)
At least four major transcripts are produced by the avian c-erbB/epidermal growth factor receptor gene. (elsevierpure.com)
The HER-2/neu gene, also known as c-erbB-2, is a member of the epidermal growth factor receptor family and acts as a proto-oncogene. (rainsureglobal.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)

Neuregulin 2, also known as NRG2, is a protein which in humans is encoded by the NRG2 gene. (wikipedia.org)

A dominant-negative form of ErbB-2 blocks Sema4D-induced RhoA activation as well as axonal growth cone collapse in primary hippocampal neurons. (rupress.org)
Our data indicate that ErbB-2 is an important component of the plexin-B receptor system and that ErbB-2-mediated phosphorylation of plexin-B1 is critically involved in Sema4D-induced RhoA activation, which underlies cellular phenomena downstream of plexin-B1, including axonal growth cone collapse. (rupress.org)
Our findings reveal ErbB activation as a strong inductive signal for stem-cell proliferation. (nature.com)
Figure 5: Lrig1 controls ErbB activation in vivo . (nature.com)
Activation of the erbB-2 receptor occurs through heterodimer formation with a ligand-bound erbB receptor family member. (bvsalud.org)
Activation of HER-2 is frequent in ductal carcinoma in situ suggesting that HER-2 signaling plays a critical role in the early stages of breast tumorigenesis. (ihcworld.com)
Therefore, we utilized quantum dot (QD)-structured single-receptor monitoring and analysis strategies in live cells to research whether a spatial control exists that may impact the activation of overexpressed HER2s in BC cells. (president2010.info)
Ligand-induced Notch receptor activation requires at least two cleavages that release the intracellular domain from the cytomembrane and allow it to translocate into the nucleus where it activates its target genes [1]. (scienceexhibitions.org)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)

The Her-2/ neu proto-oncogene, also known as c-erbB-2, is a member of the type I growth factor receptor gene family and is located in the long arm of chromosome 17 (17q12-21.32) [ 1 ]. (biomedcentral.com)

Regulation of cyclooxygenase-2 by interferon γ and transforming growth factor α in normal human epidermal keratinocytes and squamous carcinoma cells. (houstonmethodist.org)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Data obtained with these reagents demonstrate that whereas AP- 2α and AP-2γ are both present in benign breast epithelia, there is a significant up-regulation of AP-2γ expression in breast cancer specimens (P=0.01). (johnshopkins.edu)
Alterations in gene sequence or expression can occur in the cell-signalling and regulatory pathways involved in cell-cycle control, apoptosis, proteosome regulation and angiogenesis. (bmj.com)
Achieving systematic, genome-scale perturbations within intact biological systems is important for elucidating gene function and epigenetic regulation. (cdc.gov)

Shan W, Cao M, Leung D, Tyler BM: The Avr1b locus of Phytophthora sojae encodes an elicitor and a regulator required for avirulence on soybean plants carrying resistance gene Rps1b. (interleukin-receptor.com)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)

The total-ErbB-2 antibody supplied in the FACE ErbB-2 Kits recognizes ErbB-2 protein regardless of its phosphorylation site. (activemotif.com)

Also, FISH is more sensitive than IHC for detection of abnormalities in the HER-2/neu gene, and further studies should be undertaken to determine whether a FISH-based HER-2/neu detection method may prove of importance in the prediction of prognosis and planning of therapy in prostate cancer patients. (nih.gov)
The HER-2/neu onkogene may play a role in the prognosis and management of patients with breast cancer. (thieme-connect.de)
Prospective studies correlating HER-2/neu overexpression with prognosis might provide additional data. (thieme-connect.de)
5 Beckmann M W, Niederacher D, Schnurch H G, Bender H G. The erbB gene family: significance for tumor development, prognosis and new therapeutic modalities. (thieme-connect.de)
Expression of the c-erbB-2 oncoprotein appears to be an important independent indicator of prognosis in human breast cancer. (ox.ac.uk)

Once the ligand binds to the extracellular domain the receptor undergoes a conformational change of this region which allows homodimerization or heterodimerization with another activated receptor of the HER family. (ecolowood.com)

After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. (cellsignal.com)
The Y877 kit contains phospho-ErbB-2 antibody that recognizes ErbB-2 only when phosphorylated at Tyr877, while the Y1248 kit provides a phospho-antibody that recognizes ErbB-2 only when phosphorylated at Tyr1248. (activemotif.com)
Each kit contains a total-ErbB-2 antibody that recognizes ErbB-2 regardless of its phosphorylation state. (activemotif.com)
The FACE ErbB-2 (Y877) Kit contains a phospho-ErbB-2 antibody that was raised in rabbit against a synthetic phospho-peptide corresponding to residues surrounding phosphorylated Tyr877 of human ErbB-2 and recognizes ErbB-2 only when phosphorylated at Tyr877. (activemotif.com)
Sections of formalin-fixed, paraffin-embedded tissue from 185 primary breast carcinomas were stained immunohistochemically using a polyclonal antibody against the c-erbB-2 oncoprotein. (ox.ac.uk)
For continuous use, store undiluted antibody at 2-8°C for up to a week. (arigobio.com)
The 70-kDa truncated receptor could be coimmunoprecipitated from conditioned medium of transfected COS1 cells that was supplemented with recombinant human transforming growth factor alpha (TGFα) by a monoclonal antibody against human TGFα. (elsevierpure.com)
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. (neobiotechnologies.com)
We assessed Her-2/ neu alteration using CISH on formalin-fixed paraffin-embedded primary invasive ductal carcinoma tumors in which IHC (CB11 antibody) had previously been performed, and we compared the results with IHC. (biomedcentral.com)

The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
In summary, signaling through the ErbB family of receptors promotes cell proliferation and opposes apoptosis, and therefore must be tightly regulated to prevent uncontrolled cell growth from occurring. (iiab.me)

Gene expression profiling of ErbB receptor and ligand-dependent transcription. (rochester.edu)
Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. (arigobio.com)
Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. (arigobio.com)
The AP-2 transcription factors are required for normal growth and morphogenesis during mammalian development. (johnshopkins.edu)

gene located on chromosome 7p12-13. (ecolowood.com)

Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. (cancerindex.org)

2 Barnes D M, Lammie G A, Millis R R, Gullick W L, Allen D S, Altman D G. An immunohistochemical evaluation of c-erbB-2 expression in human breast carcinoma. (thieme-connect.de)
Cases of invasive breast carcinoma with the phosphorylated receptor have displayed aggressive clinicopathological features and adverse prognoses. (ihcworld.com)

Sequence analysis revealed that the 3′ end of one cDNA clone diverged from the known sequence of the extracellular ligand-binding domain (LBD) of the full-length receptor. (elsevierpure.com)
Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. (neobiotechnologies.com)

Figure 4: Lrig1 controls endogenous signalling through the ErbB pathway. (nature.com)
Major final results incorporated resolution of plasma fibrin clot components, such as clog permeability (Ks) along with performance regarding fibrinolysis making use of clog lysis period (CLT), as well as thrombin technology (prothrombin fragmented phrases 1 + 2) as well as endogenous thrombin possible (ETP) along with fibrinolysis inhibitor quantities. (hif-signal.com)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)

Through interaction with the ErbB family of receptors, NRG2 induces the growth and differentiation of epithelial, neuronal, glial, and other types of cells. (wikipedia.org)

Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). (rndsystems.com)

Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). (arigobio.com)
Dimerisation results in the autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptors and initiates a variety of signaling pathways. (iiab.me)

Sensitivity of HER-2/neu antibodies in archival tissue samples of invasive breast carcinomas. (thieme-connect.de)
About 5% of breast carcinomas are positive for phospho-c- erb B-2/HER-2/ neu oncoprotein. (ihcworld.com)
ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. (activemotif.com)
c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. (neobiotechnologies.com)
HER-2 is over-expressed in approximately 7-34% of patients with gastric cancer [20] [21] and in 30% of salivary duct carcinomas. (iiab.me)

Assessment of F-MuLV-induced tumorigenesis reveals new candidate tumor genes including Pecam1, St7, and Prim2. (rochester.edu)

Conditioned medium from these transfected cells contained a 70-kDa protein that was specifically immunoprecipitated by a polyclonal antiserum directed against the LBD of the avian c-erbB gene product. (elsevierpure.com)
ERBB is abbreviated from erythroblastic oncogene B, a gene isolated from avian genome. (iiab.me)

Expression of each cluster's gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq. (stowers.org)
Cluster identity was assigned based on the top 10 marker genes of each cluster ( Table S2 ), followed by inspection of RNA in situ hybridization patterns. (stowers.org)

The HER-2/neu gene was localized using the Oncor (Gaithersburg, MD) digoxigenin-labeled unique sequence probe. (nih.gov)
Sequence analysis of this genomic DNA fragment revealed that the 2.6-kb c-erbB transcript is produced by alternative processing. (elsevierpure.com)

Furthermore, we detected an association (P=0.04) between the expression of AP-2γ and the presence of an additional signal transduction molecule implicated in breast cancer, the insulin-like growth factor I receptor. (johnshopkins.edu)
Analysis of the proximal promoter of the insulin-like growth factor I receptor revealed a novel AP-2-binding site. (johnshopkins.edu)

Neuregulin 2 (NRG2) is a novel member of the neuregulin family of growth and differentiation factors. (wikipedia.org)
Taken together, these data strongly support a role for the AP-2 gene family in the control of cell growth and differentiation in breast cancer. (johnshopkins.edu)

Anatomy1

Diseases1

Chemicals and Drugs9

Phenomena and Processes4

Amplification32

HER220

EGFR19

Signaling pathway1

ERBB26

Proteins8

Immunohistochemistry3

Human Epidermal Growth F2

Ligands6

Estrogen receptor5

Kinase11

Epidermal Growth11

Humans1

Activation10

Growth factor r1

Regulation5

Encodes2

Phosphorylation site1

Prognosis5

Extracellular domain1

Antibody10

Apoptosis2

Transcription4

Located on chromosome1

Neoplasms1

Breast carcinoma2

Ligand bindin2

Endogenous3

Induces1

Proliferation1

ErbB31

Tyrosine residues2

Carcinomas5

Tumor genes1

Avian2

Situ Hybridization2

Sequence2

Insulin2

Differentiation2