Gene receptor clone cells. Medical search. Frequent questions

E. coli B strains are "naturally" deficient in the Lon protease which in K-12 strains serves to degrade misfolded proteins and to prevent some cell cycle-specific proteins from accumulating. (neb.com)
Cells deficient in both these proteases are much more amenable to the production of proteins from cloned genes. (neb.com)
A total of 62 dolphin genes of known stress or immune function have been cloned by targeted PCR (including immunoglobulins, T-cell receptors, cytokines, Toll-like receptors, stress proteins and toxin response proteins). (vin.com)
IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. (rndsystems.com)
Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. (rndsystems.com)
The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
Effector proteins interacting with the Fc portion of immunoglobulin M (IgM) include complement and complement receptors. (medscape.com)
Partial or complete deficiencies of the components of the complement system, including its receptors and regulatory proteins, are now described in humans and may be of a genetic or familial origin or acquired. (medscape.com)
Defects in the expression of complement or complement receptors may result in loss of tolerance to self-proteins and the development of immune complex-mediated autoimmune diseases such as systemic lupus erythematosus (SLE). (medscape.com)
The impaired function of specific organelles indicates that the causative genes encode proteins operative in the formation of lysosomes and vesicles. (medscape.com)

Molecular characterization of two novel alternative spliced variants of the KLRF1 gene and subcellular distribution of KLRF1 isoforms. (nih.gov)
This serves as a guidepost for rationally designing new combination checkpoint therapies, downstream T cell receptor (TCR) isolation, cancer antigen identification and molecular characterization of naturally occurring tumor-reactive T cells in human cancer. (upenn.edu)
To our knowledge, this is the first report on the molecular cloning of an oxytocin/vasopressin-like receptor and its ligand from arthropods. (lu.se)
High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes. (genscript.com)
The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. (nih.gov)
This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. (nih.gov)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
ST13 plays a role in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. (prospecbio.com)
Molecular characterization and gene expression of lhcb5 gene encoding CP26 in the light-harvesting complex II of Chlamydomonas reinhardtii. (bio.net)
The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)
Recent advances have been made in the of host factors, polymorphisms, and candidate genes associated characterization of the immune response to low-molecular- with occupational asthma may improve our understanding of weight agents. (cdc.gov)

Leukotriene B4 receptor 1 is a protein that in humans is encoded by the LTB4R gene. (wikipedia.org)
125 I-Bolton Hunter substance P binding would be done to verify the presence of the neurokinin receptor protein. (usda.gov)
Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled. (lu.se)
The glmS gene (glucosamine synthetase) is mutated and the expressed GlmS protein (67 kDa) contains six histidine to alanine substitutions (positions 62, 65, 432, 436, 466, 467). (neb.com)
The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. (nih.gov)
We later identified PIAS (protein inhibitor of activated STAT)xalpha as a DJ-1-binding protein, and found that DJ-1 restored androgen receptor (AR) transcription activity that was repressed by PIASxalpha. (nih.gov)
To further characterize the function of DJ-1, we cloned cDNA encoding a novel DJ-1-binding protein, DJBP, by a yeast two-hybrid system. (nih.gov)
A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. (jax.org)
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. (sigmaaldrich.com)
Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. (sigmaaldrich.com)
A novel 160-kDa intermediate filament associated protein, named restin (Reed-Sternberg intermediate filament associated protein), is specifically expressed in the malignant cells of Hodgkin's disease and anaplastic large cell lym-phoma (Ki-1 lymphoma). (karger.com)
The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. (rndsystems.com)
Maturation occurs in the cell membrane resulting in a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity ( 5 , 6 ). (spandidos-publications.com)
RON is activated in response to macrophage-stimulating protein (MSP), and then induces an invasive program ( 8 ) consisting of cell proliferation, migration, and invasion, all of which are important at multiple points during tumorigenesis. (spandidos-publications.com)
ST13 is an adaptor protein (co-chaperone) that mediates the association of HSP70 & HSP90 and appears in early receptor complexes. (prospecbio.com)
Anti-human ST13 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ST13 protein 1-369 amino acids purified from E. coli. (prospecbio.com)
The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. (rndsystems.com)
The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. (rndsystems.com)
Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
Electrophoretic mobility shift assays detected allele-dependent nuclear protein binding in A549 cells for 8 of 21 variants. (cdc.gov)
The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity. (microbiologyresearch.org)
Antibiotics (ABX) compromise the efficacy of programmed cell death protein 1 (PD-1) blockade in cancer patients, but the mechanisms underlying their immunosuppressive effects remain unknown. (bvsalud.org)

In addition, intraperitoneally administered Colivelin suppresses memory impairment caused by a muscarinic acetylcholine receptor antagonist, 3-quinuclidinyl benzilate, indicating that a substantial portion of intraperitoneally administered Colivelin passes through the blood-brain barrier and suppresses functional memory deficit. (jneurosci.org)

Genotypic pattern observed in small plaque parapsoriasis is similar to that observed in chronic dermatitis, and the pattern of clonality of T cells is consistent with the response of a specific subset of T cells that have been stimulated by an antigen. (medscape.com)
Another approach relies upon the use of chimeric antigen receptors (CARs) that directly target cancer cells or immunosuppressive cellular elements in the tumor microenvironment. (upenn.edu)
He is best known for cloning the genes of the human T cell antigen receptor and using genetically modified mice to elucidate the function of the first immune checkpoint regulator, CTLA-4. (uhnresearch.ca)
Antigen-specific T cell unresponsiveness in cloned helper T cells mediated via the CD2 or CD3/Ti receptor pathways. (ox.ac.uk)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

Genetic changes include mutations, gene silencing through epigenetics, gene amplification or deletion and whole chromosome gains or losses. (bmj.com)
Alterations in receptor tyrosine kinases (TKs), such as the epidermal growth factor receptor (EGFR) and insulin-like growth factor receptor 1, include overexpression, amplification or mutations. (bmj.com)
In this new research, investigators at PACT Pharma report on being able to use CRISPR to not only take out specific genes, but also to insert new ones in immune cells efficiently redirecting the immune cells to recognize mutations in the patient's own cancer cells. (eurekalert.org)
This is a leap forward in developing a personalized treatment for cancer, where the isolation of immune receptors that specifically recognize mutations in the patient's own cancer are used to treat the cancer" said Dr. Ribas, corresponding author of the article. (eurekalert.org)
The patients had immune cells isolated from their blood based on their binding to capture reagents that displayed up to 350 mutations from the patient's own cancer, for a total of over 5000 mutations being targeted across 34 flavors of HLA subtypes of the immune system. (eurekalert.org)
The genes of the immune receptors that allowed the immune cells to specifically recognized the cancer mutations were then sequenced, totaling 175 newly isolated cancer-specific immune receptors. (eurekalert.org)
They were then inserted back into the patient's own immune cells using a one-step CRISPR editing, which included the knock-out of the existing immune cell receptors and knock-in of the immune receptors that could redirect those cells to specifically recognize mutations in their cancer. (eurekalert.org)
Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Given that influenza A (H5N1) viruses isolated from humans are not transmitted efficiently despite their ability to recognize human-type receptors ( 14 ), mutations in the polymerase and other viral genes may also be needed for replication of influenza A (H5N1) viruses in the upper respiratory tract ( 15 ). (cdc.gov)
Although a number of studies have shown that patients with activating mutations in exons 18-21 of the epidermal growth factor receptor (EGFR) gene respond particularly well to gefitinib and erlotinib, a prospective, randomised study was needed to differentiate between the prognostic and predictive value of EGFR mutations. (ersjournals.com)
possibility is that the damage is not induces mutations in a mutation integrated viral genome ( Todaro and repaired at al , and when the cell rep- assay permits it to be classified as Huebner, 1972) , and alteration of im- licates, the DNA polymerase correct- mutagenic. (who.int)
Mutations of the Janus kinase 2 ( JAK2 ) gene are responsible for polycythemia vera and a high proportion of cases of essential thrombocythemia and primary myelofibrosis. (msdmanuals.com)
in 2016, a tenth type was defined based on mutations in the AP3D1 gene. (medscape.com)

Here we show the features of repertoire diversity and the gene expression profile of HLA-A24 cytomegalovirus-specific cytotoxic T-cells in actual recipients according to the cytomegalovirus reactivation pattern. (nature.com)
Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)
On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)
However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
Alterations in gene sequence or expression can occur in the cell-signalling and regulatory pathways involved in cell-cycle control, apoptosis, proteosome regulation and angiogenesis. (bmj.com)
Gene expression and cellular localization of neurokinin receptors would be examined by reverse transcription-polymerase chain reaction and in situ hybridization. (usda.gov)
Cloning the neurokinin receptor genes in guinea pig epithelial cells and their expression in epithelial cells lines would allow study of their function. (usda.gov)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
Expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) is strictly regulated during mammalian development and tumorigenesis. (nih.gov)
This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. (nih.gov)
Virus receptor specificities and expression patterns of receptors on host cells are thought to be major determinants of the host range restriction of influenza viruses ( 13 ). (cdc.gov)
Clones were assessed for plasma membrane expression of the individual receptor subunits by mass spectrometry and immunochemistry, and their calcium flux was measured in the presence of a library of kinase inhibitors and a focused library of acetylcholine receptor ligands. (rti.org)
We demonstrated an incorporation of two alpha 3 subunits in approximately 98% of plasma membrane receptor pentamers, indicating a 2/3 subunit expression ratio of alpha 3 to beta 4 alone or to coexpressed beta 4 and alpha 5. (rti.org)
With prolonged nicotine exposure, the plasma membrane expression of receptors with and without incorporated alpha 5 increased. (rti.org)
Whereas alpha 5 subunit expression decreased the cell calcium response to nicotine and reduced plasma membrane receptor number, it partially protected receptors from nicotine mediated desensitization. (rti.org)
This clone was engineered to express the complete ORF with an expression tag. (origene.com)
Expression varies depending on the nature of the gene. (origene.com)
Two cDNA libraries from known health status dolphin PBL have been generated including an IL-2 and an LPS-stimulated cDNA library, respectively biased towards T and B-cell gene expression. (vin.com)
The expression of ST13 is downregulated in colorectal carcinoma tissue signifying that is candidate tumor suppressor gene. (prospecbio.com)
The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa. (prospecbio.com)
In the luciferase assay 4 of the 21 SNPs exhibited allele-dependent changes in gene expression. (cdc.gov)
To define a comprehensive landscape of ICs in the human CRC tumor microenvironment (TME), we evaluated, using multiparametric flow cytometry, their ex vivo expression via tumor-infiltrating lymphocytes (TILs) (n = 40 CRCs) as well as that of their respective ligands on tumor and myeloid cells (n = 29). (bvsalud.org)
Supervised flow cytometry analyses showed that (i) most CD3+ TILs expressed PD-1 and TIGIT and, to a lesser extent, Tim-3, Lag3 and NKG2A, and (ii) EpCAM+ tumor cells and CD11b+ myeloid cells differed in their IC ligand expression profile, with a strikingly high expression of CD155 by tumor cells. (bvsalud.org)
A brief introduction to high throughput technologies for measuring and analyzing gene expression is given. (lu.se)
The main emphasis is on supervised machine learning methods for classification and prediction of tumor gene expression profiles. (lu.se)
Using such probes, one can obtain a fingerprint of the gene expression activity in a macroscopic sample. (lu.se)
Microarrays are one such tool that allows for the study of expression of thousands of genes simultaneously. (lu.se)
In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
Levels of gene expression are measured using a preparation of fluorescently labeled tissue RNA (copies of DNA), together with reference RNA labeled with a different fluorochrome, hybridized onto the slides. (lu.se)
Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)
Because the expression of the beta-3A subunit is normally ubiquitous, deficiency of the beta-3A subunit leads to a precise phenotype in cells with a large number of intracellular granules (eg, neutrophils, natural killer cells, cytotoxic T lymphocytes, platelets, melanocytes). (medscape.com)
The data presented is normalized to the expression of Rpl32 , a housekeeping gene. (cdc.gov)

The epidermal growth factor receptor (EGFR) tyrosine kinase (TK) plays an important role in the pathogenesis of NSCLC. (bmj.com)
This ~145 kDa type I transmembrane glycoprotein is encoded by Kit (KIT proto-oncogene receptor tyrosine kinase) which belongs to the receptor tyrosine kinase family (RTK) within the immunoglobulin superfamily (IgSF). (bdbiosciences.com)
Recepteur d'origine nantais (RON) is a receptor tyrosine kinase (RTK) normally expressed at low levels in epithelial cells. (spandidos-publications.com)
Janus kinase 2 is a member of the type I tyrosine kinase family of enzymes and is involved in signal transduction for the erythropoietin , thrombopoietin, and granulocyte colony-stimulating factor (G-CSF) receptors. (msdmanuals.com)

The gene is localized on the same chromosome at the distance of about 50 kb from telomeric sequences of IL-17A gene, and both genes are in a tail-to-tail orientation [ 3 ]. (hindawi.com)
A pseudogene located on chromosome 1 has been found for this gene. (nih.gov)
By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31→q24.33, while localization of the α-2-macroglobulin receptor (A2MR) was refined to 12q13.1→q13.3. (karger.com)
They are encoded by separate but linked genes termed regulation of complement activation (RCA) genes, located on the q32 region of chromosome 1. (medscape.com)
HPS-2 is caused by a mutation in the gene encoding the beta-3A subunit of the heterotetrameric AP3 complex ( ADTB3A ), which resides on chromosome 5. (medscape.com)

The CRISPR technique has been previously used in humans to remove specific genes to allow the immune system to be more activated against cancer. (eurekalert.org)
Correspondingly, epithelial cells in the upper respiratory tracts of humans mainly bear SAα2,6Gal receptors ( 10 , 11 ), and those in duck intestines (the major replication site for duck viruses) mainly possess SAα2,3Gal ( 12 ). (cdc.gov)
The receptors are widely expressed in humans. (medscape.com)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy. (nih.gov)
Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
However, cellular immune reconstitution of CMV-specific cytotoxic T-cells (CMV-CTL) is generally considered to be necessary for the long-term control of CMV reactivation after allo-HCT. (nature.com)
The identification of CMV-CTL clones is one method used to assess cellular immunity against CMV. (nature.com)
It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. (bdbiosciences.com)
We identify monocyte-derived dendritic cells (moDC) as a cellular subtype co-expressing both cytokines and validate our finding using in vitro culture systems. (bvsalud.org)
Gene therapy products and cancer vaccines are reviewed by the CBER Office of Tissues and Advanced Therapies (OTAT, formerly known as the Office of Cellular, Tissue and Gene Therapies, or OCTGT). (wcgclinical.com)
C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
The complement system, interacting with its regulatory molecules and cellular receptors, plays a central role in the induction and regulation of immunity. (medscape.com)

We have recently identified, for the first time, that bronchial epithelial cells are its novel cell source in response to IL-33 via ST2-ERK1/2-MSK1 signaling pathway. (hindawi.com)

Substance P which is found in extrinsic and intrinsic neurons is elevated along with its receptor during inflammation. (usda.gov)
The first aim addresses whether neural secretory reflexes triggered by mucosal stroking and activation of 5-HT1P receptors present on intrinsic afferent neurons stimulate chloride secretion. (usda.gov)
5 The anorexigenic effect of monoamine serotonin is also mediated by the 5HT-2C receptor in POMC neurons. (nature.com)
F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)

Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)
In this work, we extended the characterization of IL-22BP production and distribution in CD tissues by applying enzyme-linked immunosorbent assays to supernatants obtained from the culture of endoscopic biopsies of patients, and reverse transcription-quantitative polymerase chain reaction on sorted immune cell subsets. (bvsalud.org)

These three cytokines are differentially expressed in activated CD4 + T cells. (hindawi.com)
Moreover, NC16A-specific T cell clones derived from these patients were shown to express a CD4+ memory T cell phenotype and secrete cytokines that may promote autoantibody production. (uni-luebeck.de)
IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. (rndsystems.com)
Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)

As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. (rndsystems.com)

Other current efforts include the exploration of immunomodulation to potentiate endogenous antitumor T cell responses, use of bispecific antibodies, pharmacological sensitization of tumor cells to immune attack, tumor vasculature targeting, preclinical validations, clinical translation and trial support. (upenn.edu)

Another field of study is the de novo generation of tumor-reactive T cells through genetic engineering methods. (upenn.edu)
Genetic variants with gene regulatory effects are associated with diisocyanate -induced asthma. (cdc.gov)
These results demonstrate that many DA-associated genetic variants likely act by modulating gene regulation. (cdc.gov)
The principle of preferentially selecting patients most likely to benefit from therapy according to their genetic profile has led to substantial clinical benefit in some tumour types, and has potential to considerably refine treatment in advanced nonsmall cell lung cancer (NSCLC). (ersjournals.com)
These deleterious ABX effects were mimicked by oral gavage of Enterocloster species, by genetic deficiency, or by antibody-mediated neutralization of MAdCAM-1 and its receptor, α4ß7 integrin. (bvsalud.org)

We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
The following Lrrtm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
Random Expressed Sequence Tag (EST) clones have been isolated and sequenced from dolphin Peripheral Blood Leukocyte (PBL) cDNA libraries. (vin.com)
Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. (rndsystems.com)

Effects of chloroplast DNA content on the cell proliferation and aging in Chlamydomonas reinhardtii. (bio.net)
Mastocytosis and Mast Cell Activation Syndrome Mastocytosis is mast cell proliferation with infiltration of skin or other tissues and organs. (msdmanuals.com)
Although proliferation of one or more hematopoietic cell types dominates the clinical picture in each of these disorders, all three are caused by clonal proliferation of a pluripotent hematopoietic stem cell, causing an increased proliferation of normal RBC, WBC, and platelet progenitors in the bone marrow. (msdmanuals.com)

It has been generally accepted that increased levels of toxic amyloid-βs (Aβs) are closely related to AD pathogenesis, including neuronal cell death (for review, see Hardy and Selkoe, 2002 ). (jneurosci.org)

Thus, the IL-17 cytokine family appears to represent a distinct ligand-receptor signaling system. (hindawi.com)
CD117 (c-kit) serves as a receptor for c-Kit ligand (aka, stem cell factor or SCF). (bdbiosciences.com)

These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
A better and deeper understanding of immune reconstitution following allo-HCT is required for further clinical application of these adoptive T-cell therapies. (nature.com)
One focus of the Powell lab is on the generation/isolation high avidity, tumor-reactive T cells from heterogenous tumor infiltrating lymphocyte populations in traditionally "non-immunogenic" cancers utilizing novel T cell capture techniques, and to deeply examine the characteristics and function of these immune cells. (upenn.edu)
One approach relies on the isolation and cloning of T cell receptors (TCRs) that confer non-reactive T cells with specific and potent immune function following gene transfer. (upenn.edu)
NOVEMBER 10, 2022-For the first time, scientists have used CRISPR technology to insert genes that allow immune cells to focus their attack on cancer cells, potentially leaving normal cells unharmed and increasing the effectiveness of immunotherapy. (eurekalert.org)
When infused back to patients, these CRISPR engineered immune cells preferentially traffic to the cancer and become the most represented immune cells there. (eurekalert.org)
The human immune system has specific receptors on immune cells that can specifically recognize cancer cells and differentiate them from normal cells. (eurekalert.org)
These are different for every patient, so finding an efficient way to isolate them and insert them back into immune cells to generate a personalized cell therapy to treat cancer is key to making the approach feasible on a large scale. (eurekalert.org)
The new research reports on an efficient way to isolate these immune receptors from patient's own blood using new technologies developed initially through a collaboration by Dr. Ribas with James Heath, PhD, president of the Institute for Systems Biology in Seattle, and the Nobel Laureate David Baltimore, PhD, emeritus professor at Caltech and member of the UCLA Jonsson Comprehensive Cancer Center, and further developed into the clinic by PACT Pharma. (eurekalert.org)
After isolation, the immune receptors are used to redirect immune cells to recognize cancer using CRISPR gene editing. (eurekalert.org)
The generation of a personalized cell treatment for cancer would not have been feasible without the newly developed ability to use the CRISPR technique to replace the immune receptors in clinical-grade cell preparations in a single step. (eurekalert.org)
Up to three such preparations of gene edited immune cells were infused back to the patients after giving conditioning chemotherapy, for a total of 37 immune receptors being infused to the 16 patients participating in this research. (eurekalert.org)
The gene edited immune cells were preferentially recovered from biopsies of patient's post-infusion, frequently representing the top 20% of immune cells in the cancer. (eurekalert.org)
Mice homozygous for disruptions of this gene displace abnormalities of the immune system that are highly background sensitive. (jax.org)
Moreover, genes known to be important in the innate and adaptive immune responses of terrestrial mammals and in responses to stress and contaminant exposure have been targeted for cloning using PCR-based techniques. (vin.com)
The 2200 unigenes from the EST collection and the 62 immune-function targeted genes, together with other genes randomly selected without sequencing from the cDNA libraries, have been amplified and used to construct a cDNA microarray representing 3700 dolphin genes in duplicate for a total of 7400. (vin.com)
Complement and complement receptors play a critical role in immune defense by initiating the rapid destruction of invading microorganisms, amplifying the innate and adaptive immune responses, and mediating solubilization and clearance of immune complexes. (medscape.com)
They tend to enhance the effects of complement and are highly important in the binding of opsonized immune complexes on B cells. (medscape.com)
The CR1 receptor preferentially binds C3b that is covalently attached to immune complexes, and it has a weaker affinity for bound C4b and iC3b. (medscape.com)
Opsonized immune complexes (coated by C3b and C4b) bind to CR1, mostly on red blood cells, and are cleared through the liver where they can be transferred to CR3-bearing phagocytes and endocytosed. (medscape.com)
CR1 and CR2 have been shown to influence the immune environment in a B-cell receptor-independent manner. (medscape.com)
Another theory sug- perature, blood pressure, and noting signs gests that circulating immune complexes of liver cell failure such as pallor, jaundice, composed of HCV antigens and antibodies palmar erythema, fetor hepaticus and flap- deposit in tissues and initiate an inflamma- ping tremor. (who.int)

Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

Four variants exhibited effects on gene regulation (ATF rs11571537, CDH17 rs2446824 and rs2513789, and TACR1 rs2287231). (cdc.gov)
By inducing the down-regulation of mucosal addressin cell adhesion molecule 1 (MAdCAM-1) in the ileum, post-ABX gut recolonization by Enterocloster species drove the emigration of enterotropic α4ß7+CD4+ regulatory T 17 cells into the tumor. (bvsalud.org)

Novel therapeutic agents, in particular those that specifically target members of the human epidermal growth factor receptor (HER (ErbB1)) pathway, have shown encouraging therapeutic efficacy. (bmj.com)
Clinically relevant improvements in survival have been attained by administering targeted therapy to the appropriate patient population: for example, the addition of trastuzumab to standard first-line chemotherapy in patients with human epidermal growth factor receptor-positive (HER2+) metastatic breast cancer 3 . (ersjournals.com)

The third aim is to determine whether substance P activates neurokinin receptors on epithelial cells to stimulate chloride secretion and prostaglandin synthesis. (usda.gov)
Their tracheal epithelial cells reportedly bear SAα2,3Gal and SAα2,6Gal receptors ( 18 ). (cdc.gov)
RON was found to be primarily expressed in cells of epithelial origin such as colon, breast and skin ( 9 ). (spandidos-publications.com)

Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. (sigmaaldrich.com)
These studies identify pharmacological tools from two distinct classes of drugs, antagonists and modifiers that are alpha 5 and alpha 5-D398N subtype selective that provide a means to characterize the role of the CHRNAS/A3/B4 gene cluster in smoking and cancer. (rti.org)
Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. (rndsystems.com)
Four distinct complement receptors, CR1, CR2, CR3, and CR4, have been described for the surface-bound complement fraction C3 and its cleavage fragments. (medscape.com)

We discovered that 83% of T cell clones obtained from BP patients preferentially express TCRBV13, while clones derived from a PG patient express the TCRBV3 gene. (uni-luebeck.de)

The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)

Ovarian cancer cell heparan sulfate 6-O-sulfotransferases regulate an angiogenic program induced by heparin-binding epidermal growth factor (EGF)-like growth factor/EGF receptor signaling. (nih.gov)
This review considers the current evidence linking biomarker profile to efficacy of epidermal growth factor receptor (EGFR)-targeted therapy in advanced nonsmall cell lung cancer (NSCLC) and the clinical implications of recent findings. (ersjournals.com)

Building on interrogations in basic T cell biology in the lab, bench-to-bedside translational immunology is being developed, with a focus on T cell-based therapy for ovarian cancer. (upenn.edu)

However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)

Deletion of this gene confers resistance to this type of phage, but does not significantly affect the transformation or growth characteristics of the cell. (neb.com)
CDC is aware of three Monkeypox virus (MPXV) cases in California in which preliminary data show a significant deletion in the tumor necrosis factor (TNF) receptor gene. (cdc.gov)
At this point, the TNF receptor gene deletion is rare. (cdc.gov)

The ACK2 monoclonal antibody specifically binds to CD117, which is also known as Proto-oncogene c-Kit (c-kit), Stem Cell Factor Receptor (SCFR), and Mast/stem cell growth factor receptor. (bdbiosciences.com)

Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)
It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)

A) Sex differences in density of -gal+ (PR+) cells in adult PRPL/PL mice. (cdc.gov)
G) No sex difference in nuclear or soma size of PR+ VMHvl cells in PRPL mice. (cdc.gov)

NKp80 is a marker of natural killer (NK) cell maturity in secondary lymphoid tissues and supports NK cell development through a stage 4a intermediate with ILC3-associated features. (nih.gov)
Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)

diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
Weak relationships were found between c-myc amplification and the presence of lymph-node metastasis, advanced stage, DNA non-diploidy and premenopausal status, but not tumor size, estrogen receptor or progesterone receptor status, or int-2 amplification. (lu.se)

IL-17F is expressed in activated CD4 + T cells, basophils, and mast cells, three important cell types involved in allergic airway inflammation [ 3 ]. (hindawi.com)
The third aim addresses the role of mast cells in mediating secretory reflexes by comparing reflexes in a model of mast cell hyperplasia, the Trichinella spiralis model, with the beta-lactoglobulin-sensitized guinea pig model with normal mast cell numbers. (usda.gov)
Mast cell activation syndrome is increased and inappropriate activation of mast cells without clonal. (msdmanuals.com)

Multiple dominant clones can be detected by polymerase chain reaction (PCR) of T-cell receptor gene usage, which supports a reactive process. (medscape.com)

HS6ST1 Insufficiency Causes Self-Limited Delayed Puberty in Contrast With Other GnRH Deficiency Genes. (nih.gov)
Given a role of DOCK8 in T- and B-cell development/function, DOCK8 deficiency may require BMT at an earlier age, depending on penetrance. (medscape.com)
Note the image below, as well as the article Pediatric Complement Receptor Deficiency may be helpful. (medscape.com)

The majority of patients with non-small-cell lung cancer (NSCLC) present with advanced disease, with targeted therapies providing some improvement in clinical outcomes. (bmj.com)
Lung cancer is the most prevalent life-threatening cancer worldwide 1 with more than 80% being non-small-cell lung cancer (NSCLC). (bmj.com)
IL-17a-producing gammadeltaT cells and NKG2D signaling mediate bacterial endotoxin-induced neonatal lung injury: implications for bronchopulmonary dysplasia. (jax.org)
Studies link a gene cluster encoding for alpha 3 beta 4 alpha 5-D398N nicotinic acetylcholine receptors to lung cancer risk as well as link a second mutation in this cluster to an increased risk for nicotine dependence. (rti.org)
Ranking of DA-associated SNPs based on their likelihood to affect gene regulatory mechanisms in the lung yielded 21 prioritized SNPs. (cdc.gov)
Risk and nonrisk oligonucleotides were tested for binding of nuclear extracts from A549, BEAS-2B, and IMR-90 lung cell lines by using electrophoretic mobility shift assays. (cdc.gov)

Previously, we demonstrated that the NC16A domain of this autoantigen harbors key epitopes of autoantibodies and T cells, indicating that it plays an essential role in the pathogenesis of diseases. (uni-luebeck.de)

fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

KLRF1, an activating homodimeric C-type lectin-like receptor (CTLR), is expressed on nearly all natural killer (NK) cells and stimulates their cytoxicity and cytokine release (Kuttruff et al. (nih.gov)
Similarly, the IL-17 receptor family (IL-17RA-RE) is not related to any of the other known cytokine receptors [ 2 ]. (hindawi.com)
We undertook this study to investigate receptor (IL-2R) accumulation is related to whether the cytokine accumulation in PCs the leukocyte content of the platelet comc obtained from single apheresis donors was ponent and can be reduced by prestorage beyond the acceptable limits of WBC conc leukocyte depletion [ 3 ]. (who.int)

The cDNAs for Epo have been cloned from many species. (rndsystems.com)

The initiating cause of parapsoriasis is unknown, but the diseases likely represent different stages in a continuum of lymphoproliferative disorders from chronic dermatitis stimulated by activated T cells to frank malignancy of cutaneous T-cell lymphoma (CTCL). (medscape.com)
In conclusion, our results provide an advanced understanding of the characteristics of autoimmune T cells in immunobullous diseases. (uni-luebeck.de)

Nicotinic acetylcholine receptors regulate the nicotine dependence encountered with cigarette smoking, and this has stimulated a search for drugs binding the responsible receptor subtypes. (rti.org)

We observe that in a cell-intrinsic fashion, populations of mononuclear phagocytes and eosinophils express IL-22BP at the highest levels in comparison to other sources of T cells. (bvsalud.org)

In particular: how do NK cells recognize B cells that are latently infected with Epstein-Barr virus? (stanford.edu)
Are there NK cell subsets that are better equipped to recognize latent EBV? (stanford.edu)
One isolate had acquired the ability to recognize a human-type receptor. (cdc.gov)

The human RON gene was originally cloned from keratinocytes ( 1 ). (spandidos-publications.com)

[ 2 ] T cell-derived complement C3a and CD46 activation is required for Th1 induction. (medscape.com)
The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)

Untreated (dashed line histogram) or treated (solid line histogram) peripheral blood mononuclear cells (PBMC) from the BD Phosflow™ T Cell Kit Lyophilized Cells (Catalog No. 560760) were reconstituted in BD Pharmingen™ Stain Buffer (Cat. (bdbiosciences.com)

C-myc amplification, and especially a high gene copy number (greater than 5 copies), was significantly related to early recurrence and death in breast cancer, a relationship seen in both the lymph-node-negative and node-positive subcategories. (lu.se)
Gene amplification, on the other hand, is relatively infrequent and occurs mostly at low copy number, implying that tumors are heterogeneous with respect to cell clones harboring c-myc amplification. (lu.se)

New model systems are being developed to test and optimize various immunotherapy strategies, including bispecific antibodies, checkpoint inhibitors, epigenetic modifiers and adoptive T cell transfer both in vitro and in vivo. (upenn.edu)

The inotocin/receptor couple can also be found in the recently sequenced genome from the parasitic wasp Nasonia vitripennis but not in any other holometabolous insect with a completely sequenced genome (12 Drosophila species, the malaria mosquito Anopheles gambiae, the yellow fever mosquito Aedes aegypti, the silk worm Bombyx mori, and the honey bee Apis mellifera), suggesting that this neuropeptide system is confined to basal holometabolous insects. (lu.se)
As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)

Four such genes, HPS1, ADTB3A, HPS3, and HPS4, are associated with the 4 known subtypes of Hermansky-Pudlak syndrome: Hermansky-Pudlak syndrome type 1 (HPS-1), Hermansky-Pudlak syndrome type 2 (HPS-2), Hermansky-Pudlak syndrome type 3 (HPS-3), and Hermansky-Pudlak syndrome type 4 (HPS-4). (medscape.com)

As the nomenclature and description of the disease spectrum under the descriptive term parapsoriasis evolved, the primary focus has been on the distinction of whether the disorder progresses to mycosis fungoides (MF) or cutaneous T-cell lymphoma (CTCL). (medscape.com)
Early patch-stage cutaneous T-cell lymphoma. (medscape.com)

A soluble version of the type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4 and IL-13. (rndsystems.com)

In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. (nih.gov)
Conserved transcription factor-binding sites within promoter regions of human RON genes were then searched. (spandidos-publications.com)
DNA constructs were cloned into a pGL3 promoter vector for luciferase gene reporter assays. (cdc.gov)

ST13 functions to promote the efficiency of glucocorticoid receptor maturation in cells. (prospecbio.com)

In a case-control study of 867 unrelated Japanese subjects, a His161 to Arg161 (H161R) substitution in the third exon of the IL-17F gene was associated with asthma. (hindawi.com)
In this variant, a 16-base pair frame shift duplication occurs at exon 15 of the HPS1 gene. (medscape.com)

The adjacent pvt gene was co-amplified with c-myc in all tumors analyzed. (lu.se)

gene symbol (e.g. (genscript.com)

Anatomy10

Organisms3

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes37

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care1

Proteins10

Molecular11

Protein22

Antagonist1

Antigen5

Mutations13

Expression33

Tyrosine kinase4

Chromosome5

Humans4

Cellular9

Signaling pathway1

Neurons4

Characterization2

Cytokines4

CDNA clones1

Endogenous1

Genetic5

Sequence4

Proliferation3

20021

Ligand2

Immune22

Localization1

Regulation2

Human epidermal2

Epithelial3

Distinct4

Preferentially1

Kinase1

Epidermal2

Immunology1

Sequences1

Deletion3

Proto-oncogene1

Dendritic cells2

Mice2

Tissues2

Estrogen receptor2

Mast3

Polymerase1

Deficiency3

Lung6

Role in the pathogenesis1

Isolation1

Cytokine3

CDNAs1

Diseases2

Regulate1

Intrinsic1

Recognize3

Keratinocytes1

Activation2

Peripheral blood mononuclear cells1

Amplification2

Inhibitors1

Species2

Subtypes1

Cutaneous T-cell l2

Soluble1

Promoter3

Maturation1

Exon2

Tumors1

Symbol1