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  • antibody genes
  • Although antibody diversity arises mainly from apparently random combinatorial and somatic mutational mechanisms acting upon a limited number of germline antibody genes, the antibody repertoire develops in an ordered fashion during mammalian ontogeny. (caltech.edu)
  • In preparation for cloning mouse hybridoma antibody genes, a primer for reverse transcription (RT primer) and a reverse primer for RACE PCR (5′ RACE primer) were designed for H, L (κ), or L (λ) chains based on the sequence of all mouse IgG classes registered in the NCBI nucleotide database. (clontech.com)
  • kappa
  • Human IG variable heavy (V(H)) and kappa (V(κ)) genes were amplified, cognate V(H)-V(κ) gene-pairs assembled as single-chain variable fragment-Fc Abs (scFvFcs) and functional studies were performed. (nih.gov)
  • lymphoid
  • Lymphocytes belong to the lymphoid lineage and are considered as divergent from other blood cells lineages as those from the myeloid or erythroid lineage. (intechopen.com)
  • Lymphoid hematopoiesis is not trivial, because although lymphocytes are found in the bloodstream and their precursor originates in the bone marrow, they mainly belong to the separate lymphatic system, which interacts with the blood circulation. (intechopen.com)
  • the common lymphoid progenitors (CLPs) can differentiate into all types of lymphocytes but lack the myeloid potential under physiological conditions, although some myeloid-related genes can be detected in CLPs depending on experimental conditions [ 1 , 2 ]. (intechopen.com)
  • The proliferating lymphoid cells showed restricted expression of immunoglobulin (Ig) light chain and amplification of clonally rearranged V-D-J regions of Ig heavy chain gene. (nih.gov)
  • Hardy, R. R., Kemp, J. D., and Hayakawa, K. (1989) Analysis of lymphoid populations in scid mice: detection of a potential B lymphocyte progenitor population present at normal levels in scid mice by three color flow cytometry with B220 and S7. (springer.com)
  • B cells are derived from a common lymphoid progenitor (CLP) cell (lin − c- kit low Sca-1 low IL-7R + ) ( 1 ), which is limited in its ability to develop into T lymphocytes but is fully capable of developing into B cells. (pnas.org)
  • mutations
  • Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. (origene.com)
  • Those with this condition are more susceptible to infections because their body makes very few of these antibodies.This condition is inherited in an X-linked recessive pattern and is caused by mutations in the BTK gene . (nih.gov)
  • polymerase chain re
  • Clonality of the lymphoproliferation in the case was examined by immunohistochemistry and polymerase chain reaction (PCR) amplification using paraffin embedded biopsy material. (nih.gov)
  • sequence
  • The antigen-binding site is composed of the variable regions of the heavy and light chains, whereas the effector site of the antibody is determined by the amino acid sequence of the heavy chain constant region. (nih.gov)
  • The inherent variability in the upstream 5′ terminus of the H and L chains results in low sequence homology. (clontech.com)
  • diagnostic
  • The latest PCR analysis of archive peripheral blood smears confirmed B lymphocyte clonality without diagnostic criteria for lymphoproliferative disease of CLL type. (semanticscholar.org)
  • occur
  • The association of segments appears to occur at random and the theoretical number of different variable region genes that might be generated in this way is the product of the number of functional V, D and J segments, i.e. ~8262. (europeanmedical.info)
  • NCBI
  • This database is built by NCBI, and, provides only a single record for each gene/transcript. (origene.com)
  • variable region
  • A heavy chain gene contains three segments (V, D, and J) that come together to form the variable region, as well as a constant region. (nih.gov)
  • The V gene segments are longer than J or D segments and encode much of the framework of the variable-region domain, together with the first and second hypervariable regions (known as the complementarity-determining regions - CDR1 and CDR2). (europeanmedical.info)
  • Since it is the variable region on the N-terminal side of the H and L chains that determines antibody-antigen affinity, analysis of this region is of great interest. (clontech.com)
  • immune
  • Furthermore, the comprehension about lymphocytes and their contribution to the immune response will favor their application in developmental hematology and immunology. (intechopen.com)