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  • Infections
  • Single genome amplification characterized the infections of 2 unprotected animals in the gag-env immunized group, both of which had reduced acute plasma viral loads that ended as transient infections indicating partial immune control. (nih.gov)
  • particles
  • Further studies showed that TRIM22 inhibited budding of virus-like particles containing Gag only, indicating that Gag was the target of TRIM22. (nih.gov)
  • Importantly, Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing native late domains.These observations indicate that Hrs normally functions to recruit Tsg101 to the endosomal membrane.HIV-1 Gag apparently mimics this Hrs activity, and thereby usurps Tsg101 and other components of the MVB vesicle fission machinery to facilitate viral budding. (nih.gov)
  • Importantly, Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing native late domains. (nih.gov)
  • The appearance of these products was unaffected or only moderately affected by substitutions in the MHR of the Gag-Pol precursor, suggesting that the mutant Gag-Pol precursors were efficiently incorporated into viral particles. (asm.org)
  • Sequencing
  • Sequencing of gag, nef, and pol was performed in 37 epidemiologically linked recipients from Zambia at the time of identified infection and every 3 mo for the first year. (nih.gov)
  • Sequencing of the gag genes, LTRs, and untranslated regions of several ev/J proviruses revealed a high level of identity between isolates, indicating that they have not undergone significant sequence variation since their introduction into the avian germ line. (asm.org)
  • residues
  • A group of 41 peptides, each 24 amino acids long and overlapping with each other by 12 residues spanning the total gag open reading frame (orf) of HIV-1 (HTLV-IIIBH 10 isolate) were synthesized using Fmoc chemistry. (uni-regensburg.de)
  • Gag p24 (residues 261-269, GEIYKRWII) was used as a control HLA-B8 restricted peptide. (nih.gov)
  • Conservative substitution of two invariant residues (glutamine 155 and glutamic acid 159) abolished viral replication and significantly reduced the particle-forming ability of the mutant gag gene products. (asm.org)
  • antigen
  • To examine the extent of active immune response to virally encoded CEs, we analyzed human leukocyte antigen class I-associated polymorphisms in HIV-1 gag, pol, and nef genes from a large cohort of South Africans with chronic infection. (nih.gov)
  • TRIM22
  • Inhibition was associated with diffuse cytoplasmic staining of HIV Gag rather than accumulation at the plasma membrane, suggesting TRIM22 disrupts proper trafficking. (nih.gov)
  • Gene chip data on TRIM22 induction was confirmed using Northern blot analysis (Figure 1A). (nih.gov)
  • TRIM22 interacts with Gag.HOS-CD4/CXCR4 cells were co-transfected with pGag-GFP and pFLAG-TRIM22 (or empty vector control pFLAG) and immunoprecipitated with anti-GFP or anti-FLAG. (nih.gov)
  • Precipitated Gag and TRIM22 were detected by Western blotting using p24CA or FLAG antibodies. (nih.gov)
  • We immunoprecipitated a Gag-GFP fusion protein expressed in the presence of FLAG-tagged TRIM22 with anti-GFP antibodies and then performed a Western blot with anti-Flag antibodies and detected co-precipitation of FLAG-TRIM22 (Figure 7). (nih.gov)
  • Reverse immunoprecipitation using anti-FLAG pulled down Gag-GFP only when FLAG-TRIM22 was co-expressed. (nih.gov)
  • Treatment of the samples with RNaseA prior to immunoprecipitation did not interfere with the association of TRIM22 with Gag, indicating that an RNA bridge did not mediate the interaction. (nih.gov)
  • Thus TRIM22 binds specifically to HIV Gag. (nih.gov)
  • accumulation
  • The observed Env accumulation surrounding Gag assemblies, with a lower density on the actual bud, could facilitate viral spread in vivo. (nih.gov)
  • Expression of HIV-1 Gag attenuated downregulation of CXCR4, resulting in accumulation of undegraded receptors within intracellular compartments. (nih.gov)
  • amino acid seq
  • In this study, amino acid sequence variation in HIV-1 gag and nef was examined in five untreated mother-infant pairs to evaluate the potential role of CD8+ T cell responses in the evolution of the viral quasispecies. (umassmed.edu)
  • structural
  • The gag gene of HIV-1 [GenBank:Kwas selected for the study because its protein motifs and structural components are well documented. (nih.gov)
  • There is a highly specific relationship between IMRs and structural and functional aspects of the Gag polyprotein. (nih.gov)
  • CXCR4
  • Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag. (nih.gov)
  • C) HOS-CD4/CXCR4 cells were co-transfected with pGag in the absence or presence of pTRIM22 and pulse-chase analysis of intracellular Gag protein was done. (nih.gov)
  • envelope
  • This family was identified during our investigation into the origin of the novel subgroup J envelope gene that was found several years ago in unusual strains of avian leukosis virus (ALV) (called ALV-J) in England ( 46 ). (asm.org)
  • Our analysis found that 2 of 2 gag-env vaccinated but infected macaques exhibited single but distinct virus envelope lineages whereas rhesus vaccinated with gag-env-GM-CSF or HA control exhibited both single and multiple env lineages. (nih.gov)
  • motif
  • The effect of Gag was dependent on an ESCRT-I interacting motif within the C-terminal p6 region of Gag. (nih.gov)
  • A conserved P(S/T)AP tetrapeptide motif within Gag (the "late domain") binds directly to the NH2-terminal ubiquitin E2 variant (UEV) domain of Tsg101. (nih.gov)
  • Fluorescence
  • Gag-GFP localization was assessed using fluorescence microscopy. (nih.gov)
  • Forty-eight hours after release of the cycloheximide block, the localization of the Gag-GFP protein was visualized by fluorescence microscopy by taking optical slices through the center of cells (Figure 5A). (nih.gov)
  • assembly
  • Distinctive HIV-1 Gag assembly sites were readily detected and were associated with Env clusters that always extended beyond the actual Gag assembly site and often showed enrichment at the periphery and surrounding the assembly site. (nih.gov)
  • variants
  • Because there were only 2 infected animals in the gag-env vaccinated rhesus compared to 10 infected rhesus in the other 2 groups, the significance of finding single env variants in the gag-env vaccinated group could not be established. (nih.gov)
  • relatively
  • Following challenge with SIVsmE660, 5 of the 6 RhMs were infected in gag-env-GM-CSF group with relatively high PVLs that were comparable to the control group [Fig. 1C]. (nih.gov)
  • transmission
  • 2008). In 37 epidemiologically linked transmission pairs (LTPs) from Zambia, we sequenced HIV-1 gag, nef, and pol at baseline and every 3 mo for 1 yr. (nih.gov)
  • contrast
  • The SU-coding region of the subgroup J env gene, in contrast, shows only 40% identity to those of the subgroup A to E viruses ( 4 , 5 , 9 , 58 ). (asm.org)
  • Sequence
  • However, the subgroup J env gene also included regions that were not related to E51 or to any other sequence in the GenBank database, indicating that if E51-type proviruses contributed to the generation of the subgroup J env gene, there must have been other elements that contributed as well. (asm.org)
  • significantly
  • In common with two other HLA-B*3501-restricted epitopes, in Gag and Nef, that were not targeted differentially, a response toward NY10 was associated with a significantly lower viral set point. (forskningsdatabasen.dk)
  • addition
  • The results demonstrated that VSV-vectored GM-CSF abrogated the protective effect of the vaccine, since 4 of 6 animals were protected in gag-env group, whereas addition of GM-CSF reduced protection to 1 of 6 animals in gag-env-GM-CSF group as previously reported . (nih.gov)
  • plasma
  • B) Model illustrating how HIV Gag mimics the Tsg101-recruiting function of Hrs and redirects Tsg101 and the ESCRT-I complex to the plasma membrane to facilitate viral budding. (nih.gov)
  • Viral env was prepared from rhesus plasma in 3 groups of 6 immunized with vesicular stomatitis virus (VSV) vectors and boosted with Semliki forest virus (SFV) replicons expressing (a) SIVsmE660 gag-env (b) SIVsmE660 gag-env plus rhesus GM-CSF and (c) control influenza hemagglutinin protein. (nih.gov)