• Title: Regulation of the heme A biosynthetic pathway: differential regulation of heme A synthase and heme O synthase in Saccharomyces cerevisiae. (genscript.com)
  • Saccharomyces cerevisiae S288C Cox15p (COX15), partial mRNA. (genscript.com)
  • Saccharomyces cerevisiae fermentation product (SCFP) is a yeast product containing bioactive fermentation metabolites, residual yeast cells, and yeast cell wall fragments. (asas.org)
  • The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. (lu.se)
  • The lager yeast Saccharomyces pastorianus has been known to be an interspecific hybrid between Saccharomyces cerevisiae and another Saccharomyces yeast since at least 1985, but the exact nature of its parents and its proper taxonomy continued to be the subject of much debate. (wikipedia.org)
  • This was confirmed shortly after with the discovery in Argentina of the new species, Saccharomyces eubayanus, with a genome 99% identical to the non-cerevisiae portion of the S. pastorianus genome, irrefutably confirming it to be the second parental species. (wikipedia.org)
  • Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. (wikidata.org)
  • Genetic mapping of arg, cpa, car and tsm genes in Saccharomyces cerevisiae by trisomic analysis. (wikidata.org)
  • Analysis of a set of stationary phase genes in Saccharomyces cerevisiae. (le.ac.uk)
  • This thesis describes the characterisation of a set of six genes of the baker's yeast Saccharomyces cerevisiae, that are induced when a molasses-grown culture reaches the end of rapid fermentative growth. (le.ac.uk)
  • To download a certificate of analysis for Saccharomyces cerevisiae Meyen ex E.C. Hansen ( 66323 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Saccharomyces cerevisiae Meyen ex E.C. Hansen ( 66323 ) is not currently available online. (atcc.org)
  • The highly conserved translation termination factor eRF1 in Saccharomyces cerevisiae is encoded by the essential gene SUP45 . (biomedcentral.com)
  • It's a yeast that is actually a strain of Saccharomyces cerevisiae. (medlineplus.gov)
  • In Saccharomyces cerevisiae , the telomere-associated protein Ndj1 is required for bouquet formation. (biorxiv.org)
  • We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. (springeropen.com)
  • Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. (springeropen.com)
  • Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. (springeropen.com)
  • The yeast Saccharomyces cerevisiae has been used in the production of traditional fermented foods and beverages as well as in the industrial production of ethanol. (springeropen.com)
  • Genes related to the thermotolerance of S. cerevisiae have been identified by DNA microarray analyses (Eisen et al. (springeropen.com)
  • In the present study, we attempted to identify genes whose expression is correlated with the degree of thermotolerance in S. cerevisiae . (springeropen.com)
  • We compared the gene expression profiles of three S. cerevisiae strains that showed different levels of thermotolerance. (springeropen.com)
  • The yeasts used in this study ( Saccharomyces cerevisiae NFRI 3122, NFRI 3145, NFRI 3155, NFRI 3205, NFRI 3225, NFRI 3236, and NFRI 3314) were obtained from the Microbiological Bank at our institute (NFRI). (springeropen.com)
  • Saccharomyces cerevisiae S288C was used as a control strain. (springeropen.com)
  • A wild-type strain, S. cerevisiae BY4743 and the deletion strain fmp21 Δ derived from BY4743 were obtained from EUROSCARF (EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis). (springeropen.com)
  • The nucleotide sequence of chromosome I from Saccharomyces cerevisiae. (genome.jp)
  • Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. (genome.jp)
  • Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X. (genome.jp)
  • The chapter discusses several viable alternatives and their strengths and weaknesses along with the protocols used to map DNase I sensitivity in Saccharomyces cerevisiae and methidium propyl-EDTA.Fe(II) sensitivity in Drosophila melanogaster. (psu.edu)
  • A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. (nyu.edu)
  • The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. (vtt.fi)
  • Interestingly, KlSSO1 and KlSEB1 show similar enhancing effect on production of a secreted protein as the SSO and SEB1 genes of S. cerevisiae when overexpressed. (vtt.fi)
  • In this chapter, we describe a combinatorial metabolic engineering strategy utilizing an orthogonal trifunctional CRISPR system that combines transcriptional activation, transcriptional interference, and gene deletion (CRISPR-AID) in the yeast Saccharomyces cerevisiae. (illinois.edu)
  • Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p. (umass.edu)
  • In Saccharomyces cerevisiae, transcription of most of the phospholipid biosynthetic genes (e.g. (umass.edu)
  • Gene-interleaving patterns of synteny in the Saccharomyces cerevisiae genome: are they proof of an ancient genome duplication event? (biomedcentral.com)
  • Recent comparative genomic studies claim local syntenic gene-interleaving relationships in Ashbya gossypii and Kluyveromyces waltii are compelling evidence for an ancient whole-genome duplication event in Saccharomyces cerevisiae . (biomedcentral.com)
  • We focus on (1) pairwise comparison of gene arrangement sequences in A. gossypii and S. cerevisiae , (2) reconstruction of gene arrangements ancestral to A. gossypii , S. cerevisiae , and K. waltii , (3) synteny patterns arising within and between lineages, and (4) expected gene orientation of duplicate gene sets. (biomedcentral.com)
  • The existence of syntenic patterns between ancestral gene sets and A. gossypii , S. cerevisiae , and K. waltii , and other evidence, suggests that gene-interleaving relationships are the natural consequence of topological rearrangements in chromosomes and that a more gradual scenario of genome evolution involving segmental duplication and recombination constitutes a more parsimonious explanation. (biomedcentral.com)
  • The existence of an ancient whole-genome duplication (WGD) event in Saccharomyces cerevisiae [ 1 ] has been debated over the past several years. (biomedcentral.com)
  • In both studies, regions of 'double synteny' (DS) were identified in which single genes or groups of genes expressed homology relationships with alternating chromosomal regions of S. cerevisiae . (biomedcentral.com)
  • Although the vast majority of protein-encoding genes (95%) show homology to S. cerevisiae genes, only ~10% are gene duplicates in DS patterns. (biomedcentral.com)
  • Petes, Thomas D. / The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy suppressor of mec1 telomeric silencing defects . (uky.edu)
  • Craven, RJ & Petes, TD 2001, ' The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy suppressor of mec1 telomeric silencing defects ', Genetics , vol. 158, no. 1, pp. 145-154. (uky.edu)
  • Saccharomyces cerevisiae showed only minor alterations in growth and gene expression when its 16 chromosomes were combined. (the-scientist.com)
  • Researchers fully engineer a biochemical pathway that turns a sugar into an opioid in Saccharomyces cerevisiae . (the-scientist.com)
  • Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae. (wikigenes.org)
  • Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae. (wikigenes.org)
  • A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae. (bvsalud.org)
  • We have studied the presence of long-range correlations in the complete genomes of ten different dsDNA viruses and Saccharomyces cerevisiae (bakers' yeast) chromosome I. We have also studied the correlation between the distribution of the gene length and the domain of "1/f region" of their genomes. (who.int)
  • Saccharomyces cerevisiae (often known as budding yeast, or brewers yeast) is a single-celled micro- organism that is easy to grow and genetically manipulate. (lu.se)
  • Probiotic strains of Saccharomyces have been shown to have these abilities. (mygenefood.com)
  • Although the optimal temperature for most strains of Saccharomyces range from 22-30°C, S. boulardii survives best at 37°C, giving it a unique advantage of being one of the few yeasts that do best at human body temperatures. (mygenefood.com)
  • These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. (nyu.edu)
  • Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. (nyu.edu)
  • We conducted a multicopy suppressor screen for genes that suppress the loss of silencing in strains overexpressing Mec1p. (uky.edu)
  • This research is directed to understand how disease processes can be impacted by heritable changes in chromosome structure that influence the expression of genes affecting disease. (grantome.com)
  • The rationale for the isolation of such genes was that their promoters may be useful in a biotechnological application, where the regulated expression of heterologous proteins is required. (le.ac.uk)
  • The interactions of proteins with chromosomal DNA control a variety of cellular processes, including gene transcription, DNA packing, replication, recombination, and DNA repair. (psu.edu)
  • To implement this system, three orthogonal Cas proteins were utilized: dLbCpf1 fused to a transcriptional activator, dSpCas9 fused to a transcriptional repressor, and SaCas9 for gene deletion. (illinois.edu)
  • Ribosomes have been suggested to directly control gene regulation, but regulatory roles for ribosomal RNA (rRNA) remain largely unexplored. (nih.gov)
  • Together, these studies unravel unexpected gene regulation directly mediated by rRNA and how ribosome evolution drives translation of critical developmental regulators. (nih.gov)
  • One of the most promising techniques to optimise cell factory performance is to control the expression/repression of relevant genes using dynamic regulation. (biomedcentral.com)
  • Previous efforts to implement dynamic regulation under industrially relevant conditions required the knockout of galactose utilization genes so that a small amount of galactose can be added to fermentations as a gratuitous inducer for gene expression [ 6 ]. (biomedcentral.com)
  • In synthetic biology, the strength of promoter elements is the basis for precise regulation of target gene transcription levels, which in turn increases the yield of the target product. (biomedcentral.com)
  • Hsp26, Hsp78, Hsp82) decreased the production of ethyl acetate, suggesting that the Hsp family was also involved in the regulation of ATF1 gene transcription. (biomedcentral.com)
  • Thus, we demonstrate novel regulation of the PIS1 gene by Ume6p. (umass.edu)
  • FAE ( Fatty Acid Elongation ) like genes take part in the biosynthesis of VLCFAs, growth regulation, and stress responses, and are further comprised of KCS ( Ketoacyl-CoA synthase ) and ELO ( Elongation Defective Elongase ) sub-gene families. (nature.com)
  • This strain was admitted to the Centraalbureau voor Schimmelcultures (CBS) in 1935 as strain CBS 1538, Saccharomyces pastorianus Reess ex Hansen 1904. (wikipedia.org)
  • Saccharomyces boulardii (S. boulardii) is a type of probiotic ("friendly" organism). (medlineplus.gov)
  • Consequently, the WGD scenario requires that almost an entire gene complement be deleted, and that deletion within regions (termed 'blocks') establishing DS patterns proceed with precision and without leaving massive evidence of relics (gene remnants) or pseudogenes (Fig. 1A ). (biomedcentral.com)
  • Overexpression of these genes can rescue temperature‐sensitive (ts) growth defect of many sec mutants impaired in protein secretion. (vtt.fi)
  • Design and construction of an optimal microbial cell factory typically requires overexpression, knockdown, and knockout of multiple gene targets. (illinois.edu)
  • RNA-sequencing analysis showed that the overexpression of differential gene HSP30 increased the transcriptional abundance of ATF1 gene and production of ethyl acetate. (biomedcentral.com)
  • Furthermore, the results proved that the Hsf1, an upstream transcription factor of Hsps, had a positive effect on alleviating the unfolded protein response and that overexpression of Hsf1 reprogramed the pattern of ATF1 gene transcript levels. (biomedcentral.com)
  • Six cDNA clones, which showed differential hybridisation to mRNAs of exponential and stationary phase cells, were used as probes to recover the cognate genes from a library of yeast genomic DNA fragments carried in the bacteriophage vector, EMBL3. (le.ac.uk)
  • The following COX15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
  • Determine the use of Sb probiotic yeast among patients with positive Saccharomyces culture findings in samples other than blood, according to a retrospective registry study of all Saccharomyces sp. (cdc.gov)
  • Analyses of genes whose expression is induced by high temperature are important for understanding the mechanisms of thermotolerance, and for the use of the genes in breeding thermotolerant yeasts. (springeropen.com)
  • The QTL map contained MKT1 , END3 , and RHO2 as high-temperature growth quantitative trait genes. (springeropen.com)
  • A further two, previously undescribed genes, encode a second small, 12kDa, stress-induced protein (HSP12) and a thiamine biosynthetic enzyme (THI4). (le.ac.uk)
  • DNA sequence analysis revealed the THI5 gene to encode a second thiamine biosynthetic enzyme homologous to the product of the Schizosaccharomyces pombe gene nmtl. (le.ac.uk)
  • Transcription of these six genes has been examined under a variety of growth conditions and in various mutant backgrounds. (le.ac.uk)
  • However, the results of many researches proved that excessive transcription levels of target genes actually reduced the yield of the target product. (biomedcentral.com)
  • In this study, promoters PGK1p and TDH3p with different strengths were used to regulate the transcription level of alcohol acetyl transferase encoding gene ATF1 . (biomedcentral.com)
  • The fine-tuning and precise control of key gene transcription levels are the important basis for the successful implementation of these strategies. (biomedcentral.com)
  • It is beneficial to improve the protein expression and product yield by controlling the promoter strength to fine regulate the transcription level of the target gene and maximize the transcription efficiency. (biomedcentral.com)
  • String-based protein-protein interaction analysis suggested that bZIP53, a transcription factor might be involved in the activation of transcription of ELO / KCS genes. (nature.com)
  • Both of the thiamine genes, THI4 and THI5, are subject to repression by exogenous thiamine and become derepressed on depletion of the vitamin from the growth medium. (le.ac.uk)
  • Using sucrose as an activator of gene expression circumvents the need for expensive inducer compounds and enables gene expression to be triggered during growth on a fermentable, high energy-yield carbon source. (biomedcentral.com)
  • The ability to fine-tune the timing and population density at which gene expression is activated from the SUC2 promoter was demonstrated by varying the ratio of glucose to sucrose in the growth medium. (biomedcentral.com)
  • High concentrations of fermentable carbon sources repress gene expression from galactose promoters (GAL) via a carbon catabolite repression mechanism [ 7 ], and galactose utilisation genes are not activated during growth on sucrose [ 8 ]. (biomedcentral.com)
  • URA3 null mutant exhibits growth characteristics typical of other VMA disruptant mutants in genes encoding subunits of the catalytic sector. (tau.ac.il)
  • Starting from simple modelling of individual growth curves, a Bayesian hierarchical model can be built with variable selection indicators for inferring pairs of genes that genetically interact. (lu.se)
  • Blood cyclooxygenase-2 and myeloperoxidase mRNA gene expression was affected by diet during transport stress, being increased in control dogs, but stable or decreased in dogs fed SCFP. (asas.org)
  • The PRP17 gene product is required for the second step of pre-mRNA splicing reactions. (iisc.ac.in)
  • Replacing yeast genes with their human equivalents reveals functional conservation despite a billion years of divergent evolution. (the-scientist.com)
  • Our research also focuses on the synthesis mechanisms of telomerase, and aims for the identification and functional analysis of the genes that regulate telomerase. (lu.se)
  • During the explosion of scientific mycological studies in the 19th century, the yeast responsible for producing these so-called "bottom fermentations" was finally given a taxonomical classification, Saccharomyces pastorianus, by the German Max Reess in 1870. (wikipedia.org)
  • Synthetic biologists introduce bacterial and poppy plant genes into yeast to manufacture morphine. (the-scientist.com)
  • We identified SCS2 (suppressor of choline sensitivity), a gene previously isolated as a suppressor of defects in inositol synthesis. (uky.edu)
  • The name S. carlsbergensis is typically attributed to Emil Christian Hansen from the era when he worked for the Danish brewery Carlsberg in 1883, but in actuality it was not officially described by Hansen as a distinct species until 1908, along with another synonym, Saccharomyces monacensis. (wikipedia.org)
  • In a further publication in 1908, Hansen reclassified the original "Unterhefe Nr. I" as the new species Saccharomyces carlsbergensis and another yeast "Unterhefe Nr. II" as the new species Saccharomyces monacensis. (wikipedia.org)
  • We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. (nyu.edu)
  • A single transcript chosen for a gene which is the most conserved, most highly expressed, has the longest coding sequence and is represented in other key resources, such as NCBI and UniProt. (ensembl.org)
  • Over time U-triangle developed on polyploidization of Brassica 's proved its usefulness in studying the evolution of various genes and phenotypes 3 . (nature.com)
  • However, a viable nonsense mutant has been isolated for the prfB gene of S. typhimurium encoding RF2 release factor. (biomedcentral.com)
  • This application for a MIRA award seeks to consolidate the research in the PI's lab, funded by GM31105 Genetic Analysis of Genes Controlling a Position Effect, currently in its 37th year, and by GM120374 Metabolism and Epigenetics, currently in its fourth year, into one program. (grantome.com)
  • Therefore the aims of this project were to identify the unknown members of this set of genes and to determine the transcriptional regulatory mechanisms acting on all six. (le.ac.uk)
  • Thus, although these genes show apparent coordinate induction they are regulated via different transcriptional control mechanisms. (le.ac.uk)
  • The long legacy of GM31105 enabled the discovery of how the Sir genes control heterochromatin formation, the role of silencers in controlling gene expression, and the epigenetic inheritance of transcriptional states. (grantome.com)
  • Using a randomized crossover study design, 16 adult pointer dogs were used to compare changes in fecal characteristics, oxidative stress marker concentrations, and gene expression when fed a SCFP-supplemented diet or control diet. (asas.org)
  • Engineering dynamic, environmentally- and temporally-responsive control of gene expression is one of the principle objectives in the field of synthetic biology. (biomedcentral.com)
  • This yeast's complicated genome appears to be the result of hybridisation between two pure species in the Saccharomyces species complex, a factor that led to difficulty in establishing a proper taxonomy of the species. (wikipedia.org)