• PACE essentially comprises a system by which E. coli cells continuously flow through a fixed-volume vessel, or lagoon, which contains a replicating population of phage DNA vectors (the selection phage) encoding the gene or genes of interest. (wordpress.com)
  • To ensure that positive cDNA clones are successfully isolated from complex libraries in a single round of selection, functional screens using mammalian cells have been restricted to cellular assays in which the background of false-positive cells meeting a selection criterion is low, typically 1 in 10 5 false-positive cells. (pnas.org)
  • Mammalian expression cloning methods that permit multiple rounds of selection and enrichment have been extremely successful in identifying cDNA clones from plasmid libraries that correspond to unknown receptors or ligands ( 7 ). (pnas.org)
  • Here we describe a strategy, cyclical packaging rescue (CPR), which allows rapid recovery and retransmission of retroviral cDNA libraries. (pnas.org)
  • Retroviral cDNA libraries have been used in a number of functional screens to identify unique mammalian genes that regulate cellular responses ( 1 - 6 ). (pnas.org)
  • Here we describe an alternative method called cyclical packaging rescue (CPR) that uses direct repackaging of retroviral RNAs into new infectious virions to identify genes regulating functional responses in mammalian cells (Fig. 1 A ). In CPR, stably integrated helper-free retroviral libraries are recovered rapidly from mammalian cells as infectious helper-free retroviral supernatants 24 h after infection with adenoviruses expressing retroviral gag-pol and env genes. (pnas.org)
  • When performed in concert with selection using functional assays, cDNAs regulating functional responses can be identified by enrichment through multiple rounds of retroviral library recovery and retransmission. (pnas.org)
  • Scientists have created the first retroviral CRISPR-Cas9 gene editing library to examine the regulation of mouse T cells, it was announced last week. (b-s-h.org.uk)
  • Dr Xi Chen, joint first author from the Wellcome Sanger Institute, said while previous studies have mostly examined one gene at time, the development of a retroviral CRISPR library has enabled them to knock-out each individual gene in mouse T helper cells for the first time. (b-s-h.org.uk)
  • The fonds was donated to University of Manitoba Archives & Special Collections by Gene Telpner in 1991. (umanitoba.ca)
  • The Library of Congress maintains one of the largest and most diverse collections of scientific and technical information in the world. (loc.gov)
  • The Science, Technology and Business Division provides reference and bibliographic services and develops the general collections of the Library in all areas of science, technology, business and economics, with the exception of clinical medicine and technical agriculture, which are the subject specialties of the National Library of Medicine and the National Agricultural Library. (loc.gov)
  • Screening Screening to isolate one particular clone from a gene library routinely involves using a nucleic acid probe for hybridization. (powershow.com)
  • None of the genes identified was represented by more than one clone. (arvojournals.org)
  • Traditionally, clone libraries have been compared by describing differences in phylogenetic distributions and diversity indices (e.g., see references 3 , 10 , 18 , and 20 ). (asm.org)
  • The revolution in the development of DNA microarray technology for examining gene expression has created a new era for further exploration of living systems, source of disease and drug development (He & Hui, 2009). (igi-global.com)
  • The regulation of P450 gene expression has been well documented in experimental animals, but at present there is very little information available about the regulation of human P450 genes, particularly in extra-hepatic tissues. (bl.uk)
  • Regulation of P450 expression by a range of xenobiotics, known to have profound effects on the expression of rodent P450 genes, has been studied in a mouse model and in cultured cells. (bl.uk)
  • A model was developed, using human tumours grown as xenografts in immune deficient mice, in which the in vivo regulation of human P450 genes could be examined. (bl.uk)
  • TCPOBOP was shown to be equally effective at influencing human P450 gene expression and, in most cases, the patterns of gene regulation observed in experimental animals were also seen in the human tumours. (bl.uk)
  • RNAi2011: Gene Regulation by Small RNAs, the 6 th international Oxford RNAi conference, was held at St Hilda's College, University of Oxford (UK), between 29 and 31 March, 2011. (libpubmedia.co.uk)
  • Regulation of aldose reductase gene expression in renal cortex and medulla of rats. (bl.uk)
  • They found many genes involved in the regulation of Th2 development, and identified the transcription factor PPARG as being particularly important. (b-s-h.org.uk)
  • This has not only allowed us to identify genes involved in the regulation of Th2 cells but could also be used to study other mouse immune cells and their regulation. (b-s-h.org.uk)
  • The post-transcriptional and/or post-translational regulation of genes involved in several biological processes is certainly important to adapt the parasite to survive in the harsh conditions of the parasitophorous vacuole and to circumvent the host's immune response. (springer.com)
  • Vol. 19, Issue 4, Human Metallothionein Expression under Normal and Pathological Conditions: Mechanisms of Gene Regulation Based on In silico Promoter Analysis. (begellhouse.com)
  • You and other family members can then be tested for that specific gene change. (wellspan.org)
  • Sets of siRNAs focused on a specific gene class, also called siRNA libraries, have the capacity to greatly increase the pace of pathway analysis and functional genomics. (thermofisher.com)
  • Users can select articles or chapters that meet their interests and gain access to the full content permanently in their personal online InfoSci-OnDemand Plus library . (igi-global.com)
  • Discovering the function of a gene requires cloning a DNA sequence and expressing it. (eurekalert.org)
  • Genes regulating responses in mammalian cells are often difficult to identify by functional cloning strategies limited to a single round of selection. (pnas.org)
  • Unfortunately, the requirement for large T antigen-driven replication of plasmid libraries in mammalian cells has severely limited adaptation of expression cloning methods to functional screens with only highly transfectable cell lines. (pnas.org)
  • Molecular cloning of a group of mouse pancreatic islet beta-cell-related genes by random cDNA sequencing. (bl.uk)
  • The T47D cell ds cDNA was generated by SMART TM technology and the library was constructed using in vivo recombination-mediated cloning in the AH109 yeast strain using a pGADT7-Rec plasmid. (go.jp)
  • Isogenica's CIS display technology is an in vitro display technology that allows the rapid generation of polypeptide and antibody libraries from which it is possible to select lead molecules with high affinity and specificity for most targets. (businesswire.com)
  • With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose GENEWIZ for their partially (NNK) or completely randomized (NNN) nucleotide libraries. (genewiz.com)
  • Moving beyond some of the alarming sci-fi scenarios of gene editing, groundbreaking scientists are harnessing the power of these biological breakthroughs to save lives. (lfla.org)
  • The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. (asm.org)
  • Libraries of 16S rRNA genes provide insight into the membership of microbial communities. (asm.org)
  • To contribute to a growing statistical framework for comparing 16S rRNA libraries, we present a computer program, ∫-LIBSHUFF, which calculates the integral form of the Cramér-von Mises statistic. (asm.org)
  • Using ∫-LIBSHUFF, we found that previously published 16S rRNA gene libraries constructed from Scottish and Wisconsin soils contained different bacterial lineages. (asm.org)
  • The use of 16S rRNA gene libraries to describe microbial communities continues to provide powerful insights into microbial ecology. (asm.org)
  • As environmental microbiologists attempt to understand the ecological mechanisms that underlie differences between 16S rRNA gene libraries, robust statistical tools will be necessary. (asm.org)
  • Sry is believed to act as a switch in the complex gene mechanism that diverts mammalian development from its usual course - the female - to the male. (sciencephoto.com)
  • Complex interactions between large numbers of genes frequently govern traits and behavior. (healthcanal.com)
  • Published online in the journal Cell , the work focuses on the complex control mechanism, describing how different genes are involved in both activation and development of T helper cells. (b-s-h.org.uk)
  • For this study, we attempted to produce dsRNA of the chitin deacetylase (CDA) region of the lgx-1 gene in vitro to be used for RNAi experiments by soaking. (tufts.edu)
  • Poly(2-(dimethylamino)ethyl methacrylate)-based polymers for the delivery of genes in vitro and in vivo / F. J. Verbaan, D. J. A. Crommelin, W. E. Hennink and G. Storm -- Ch. 9. (princeton.edu)
  • Until now, such genome-wide RNAi screens have mostly been restricted to cultured cells and ubiquitous gene inactivation in Caenorhabditis elegans. (nih.gov)
  • Our RNAi transgenes consist of short gene fragments cloned as inverted repeats and expressed using the binary GAL4/UAS system. (nih.gov)
  • These results suggest that CPR is a versatile method that permits functional identification of both wild-type and dominant-negative gene products that regulate cellular responses. (pnas.org)
  • refs) PCR-based expression analysis and identification of microRNAs David P Lu †, Rebecca L Read †, David T Humphreys ‡, Fiona M Battah †, David I K Martin ‡ and John E J Rasko †§* † Gene. (libpubmedia.co.uk)
  • It allowed analyzing the expression of a maximal number of Leishmania genes, the delimitation of the 3' UTR of 478 genes and the identification of biological processes that are differentially modulated during the promastigote to amastigote differentiation. (springer.com)
  • Hence, a systematic identification of these genes is necessary to understand the mechanisms underlying parasite intracellular survival. (springer.com)
  • The research team created a new genome-wide CRISPR library of 88,000 guide RNAs that enabled them to switch off each of the 20,000 genes from mouse Th2 cells. (b-s-h.org.uk)
  • Kostas Kostarelos (University of London, UK) described how carbon nanotubes, which are internalised by the cell without apparent damage to cellular structure, could be chemically functionalised and combined with siRNAs to form gene-silencing complexes that are effectively delivered to target cells. (libpubmedia.co.uk)
  • mRNA Silencing by 178 Kinase siRNAs from the Silencer ™ Kinase siRNA Library. (thermofisher.com)
  • Several experimental approaches including SAGE (Serial analysis of gene expression) have been developed in order to investigate the parasite transcriptome organisation and plasticity. (springer.com)
  • The SOAP-based DGD API can only use Ensembl gene IDs as input data and does not allow the retrieval of cross-references in the output file. (programmableweb.com)
  • Pluronic block copolymers for nonviral gene delivery / Alexander V. Kabanov, Srikanth Sriadibhatla and Valery Yu. (princeton.edu)
  • Glycopolymer tools for studying targeted nonviral gene delivery / Kevin G. Rice, Je-Seon Kim and Dijie Liu -- Ch. 33. (princeton.edu)
  • The lgx-1 gene in C. elegans codes for a putative chitin deacetylase and has been shown to be expressed in the pharynx of the organism by in situ hybridization experiments (Kohara, 2010). (tufts.edu)
  • Here is an example of the power of Ambion's Silencer™ Kinase siRNA Library, as demonstrated through preliminary screening experiments carried out by Cenix BioScience GmbH. (thermofisher.com)
  • Baltimore deserves praise him for orchestrating an important three-day conversation that was truly international on a morally, culturally divisive issue - determining appropriate use of emergent technology capable of altering genes in a way that might cure diseases such as HIV, hepatitis-B and sickle cell anemia. (georgetown.edu)
  • Learning more about these interactions could help researchers tease out links between certain genes and certain diseases, for example. (healthcanal.com)