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  • rRNA gene
  • Adequate characterization of environmental and host-associated samples remains a challenge for microbiologists, despite the advent of 16S rRNA gene sequencing. (asm.org)
  • Instead, an alternative community profiling approach involves surveying distributions of the small subunit rRNA gene due to its ubiquity across all domains of life (16S rRNA in the Bacteria and Archaea and 18S rRNA in the Eukarya ) ( 27 ). (asm.org)
  • Additionally, the 16S rRNA gene provides valuable phylogenetic information ( 6 ) for comparison to database collections. (asm.org)
  • The genes coding for it are referred to as 16S rRNA gene and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. (wikipedia.org)
  • Some (hyper)thermophilic archaea (i.e. order Thermoproteales) contain 16S rRNA gene introns that are located in highly conserved regions and can impact the annealing of "universal" primers. (wikipedia.org)
  • As a result, 16S rRNA gene sequencing has become prevalent in medical microbiology as a rapid and cheap alternative to phenotypic methods of bacterial identification. (wikipedia.org)
  • Illumina
  • While cheaper and allowing for deeper community coverage, Illumina sequencing only produces reads 75-150 base pairs long, and has no established protocol for reliably assembling the full gene in community samples. (wikipedia.org)
  • synthesis
  • This support from the TSB follows the successful development by Isogenica of its Colibra™ technology for the commercial synthesis of ratio controlled gene libraries using a patent protected methodology discovered by Dr Anna Hine of Aston University which was also supported by TSB funding. (businesswire.com)
  • The funding enables Isogenica to develop a robust, automated manufacturing process for the commercial synthesis of ratio controlled gene libraries using a patent protected methodology invented by Dr Anna Hine and her team at Aston University. (isogenica.com)
  • MOLECULAR
  • In a report in the current online issue of the journal Nature Methods, Dr. Hugo Bellen (http://flypush.imgen.bcm.tmc.edu/lab/), a professor of molecular and human genetics at BCM and a Howard Hughes Medical Institute investigator, and his colleagues describe the new libraries. (innovations-report.com)
  • This approach is called reverse genetics and it is a powerful and sensitive tool to study the function of genes and, when combined with studies in higher plants such as Arabidopsis thaliana, can be used to study molecular plant evolution. (wikipedia.org)
  • marker
  • In this case, a gene cassette with a dual selectable marker can be incorporated into the DNA at the specific location of mutagenesis. (wikipedia.org)
  • The embryonic stem cells that incorporated the knocked-out gene are isolated from the unaltered cells using the marker gene from step 1. (wikipedia.org)
  • In order to ensure growth of only transformed bacteria (which carry the desired plasmids to be harvested), a marker gene is used in the destination vector for selection. (wikipedia.org)
  • So, for example, the "marker gene" could be for resistance to the antibiotic ampicillin. (wikipedia.org)
  • synthetic biology
  • CAMBRIDGE, England--( BUSINESS WIRE )--Today, Monday 30th June, 2014, Isogenica, Cambridge UK and Imperial College London secure funding from the Technology Strategy Board (TSB), the UK's innovation agency, for a £347,000 project to optimise a gene library assembly technology for potential use in synthetic biology. (businesswire.com)
  • We are now interested in further optimising the technology for high throughput, automated manufacture of whole gene libraries and we believe that this could represent a powerful and valuable tool for commercialisation in the synthetic biology arena" said Kevin Mathews, CEO of Isogenica. (businesswire.com)
  • genetic
  • Moreover, cells that do not integrate any of the genetic material test negative for both genes and therefore die as a result of poisoning with neomycin. (wikipedia.org)
  • Yet, T-even phages are in fact among the largest and highest complexity virus, in which these phages genetic information is made up of around 160 genes. (wikipedia.org)
  • Search
  • The following is a list of libraries from ProgrammableWeb's Library Directory that matched your search term. (programmableweb.com)
  • Although there many different interpretations of the word "library" among software developers, ProgrammableWeb adheres to a specific definition so as to clearly distinguish libraries from SDKs and frameworks in a way that will facilitate clean search results. (programmableweb.com)
  • retrieval
  • The SOAP-based DGD API can only use Ensembl gene IDs as input data and does not allow the retrieval of cross-references in the output file. (programmableweb.com)
  • In this paper, we propose an effective gene retrieval technique based on LPP and PCA called LPCA. (igi-global.com)
  • University
  • Isogenica, Cambridge UK and Aston University, Birmingham UK secure funding from the Technology Strategy Board, the UK's innovation agency, for a £0.5M project to develop a gene library assembly technology exclusively licensed to Isogenica. (isogenica.com)
  • University of New Mexico, University Libraries, Center for Southwest Research. (wikipedia.org)
  • The Eugene C. Eppley Administration Building is located on the University of Nebraska at Omaha north campus in Omaha, Nebraska. (wikipedia.org)
  • After attending an Omaha University football game with then-college president Milo Bail in 1949, Gene Eppley was asked what the growing university needed. (wikipedia.org)
  • The overall growth of the university in the years after the building's dedication is attributed to the opening of the library, as well. (wikipedia.org)
  • 1995
  • Funeral services for Gene Charles Rejda, age 54, were held April 30, 1995, at Bader Funeral Home in Lusk with Rev. Del Cowley officiating. (niobraracountylibrary.org)
  • recombination
  • citation needed] At its inception, SCOPE was developed as a homology-independent recombination technique to enable the creation of multiple crossover libraries from distantly related genes. (wikipedia.org)
  • Cells that were transformed with a vector containing the neomycin resistance gene and the herpes tk+ gene are grown in a solution containing neomycin and Ganciclovir in order to select for the transformations that occurred via homologous recombination. (wikipedia.org)
  • opens
  • She intends it to convey a double meaning-the library opens its doors to anyone who wants to learn, and one is drawn in by the hint of interesting things inside. (easternct.edu)
  • construct
  • In addition, a second gene, such as herpes tk+, is also included in the construct in order to accomplish a complete selection. (wikipedia.org)
  • methods
  • As mosses are haploid organisms, the regenerating moss filaments (protonemata) can be directly assayed for gene targeting within 6 weeks using PCR methods. (wikipedia.org)
  • multiple
  • This method incorporates indexed primers to enable the characterization of multiple microbial communities in a single flow cell lane, may be modified readily to target other variable regions or genes, and demonstrates unprecedented and economical access to DNAs from organisms that exist at low relative abundances. (asm.org)
  • exclusively
  • Recently I resubscribed to the Safari online tech library (a lot of O'Reilly titles, but not exclusively) after a hiatus of a few years. (gnxp.com)