• The identified genes and species fulfil essential functions in the ecosystem," Paul Wilmes explains: "Many metabolic pathways and species are dependent on them. (sciencedaily.com)
  • Mediators of gene silencing pathways, microRNA (miRNA) populations have now been extensively characterised and implicated in numerous fundamental processes. (edu.au)
  • These lists are commonly analyzed using software tools and databases that map genes to known pathways or construct subnetworks that connect input lists of genes using known protein-protein or other types of molecular interactions [ 1 - 10 ]. (biomedcentral.com)
  • The objective of this work is to identify dysregulated genes and pathways of ccRCC temporally according to systematic tracking of the dysregulated modules of reweighted Protein-Protein Interaction (PPI) networks. (hindawi.com)
  • Finally, gene compositions of altered modules were analyzed, and pathways enrichment analyses of genes in altered modules were carried out based on Expression Analysis Systematic Explored (EASE) test. (hindawi.com)
  • We successfully identified dysregulated genes and pathways of ccRCC in different stages, and these might be potential biological markers and processes for treatment and etiology mechanism in ccRCC. (hindawi.com)
  • Delineating the pathogenesis of ccRCC by investigating the gene and epigenetic changes and their effects on key molecules and their respective biologic pathways is of crucial importance for the improvement of current diagnostics, prognostics, and drug development [ 4 ]. (hindawi.com)
  • In addition, a PPI network can be modeled as an undirected graph, where vertices represent proteins and edges represent interactions between proteins, to prioritize disease associated genes or pathways and to understand the modus operandi of disease mechanisms [ 9 , 10 ]. (hindawi.com)
  • By subjecting DEGs to gene ontology enrichment analysis, pathway analysis, or functional annotation, we gain insights into the biological processes, molecular functions, and pathways associated with the observed gene expression changes. (pharmiweb.com)
  • The goal of our search was to identify publications describing the role of specific genes, gene signatures, or molecular pathways and their effects in response to environmental exposures. (cdc.gov)
  • Whether you're targeting specific genes or performing a genome-wide screen, we offer extensive pooled and arrayed formats to meet your needs. (sigmaaldrich.com)
  • Phylogenetic trees for specific genes in Omnitrophota genomes. (figshare.com)
  • This alignment process enables the determination of the origin of each read, allowing us to associate it with specific genes or genomic regions. (pharmiweb.com)
  • With decades of gene editing experience and an unsurpassed portfolio of CRISPR and ZFN technology patents, Sigma-Aldrich® Advanced Genomics delivers the highest quality reagents for your entire workflow, from early-stage discovery to translational research. (sigmaaldrich.com)
  • Sigma-Aldrich® Advanced Genomics RNAi Single Clones deliver powerful, long term, and stable gene silencing. (sigmaaldrich.com)
  • We are one of the few groups worldwide that can apply functional genomics to the study of smORFs, due to our expertise in functional genetic analysis, bioinformatics, and ribosomal profiling. (cabd.es)
  • Six among the 42 genes identified in this study are on the list of the top 100 persistence targets selected by the TB Structural Genomics Consortium. (hindawi.com)
  • The limitation of current population genomics-based methods for determining LOF intolerance is they are underpowered when predicting genes that are short in length. (biomedcentral.com)
  • Our method integrates a population genomics-based approach with a functional genomics approach, providing unparalleled ability to predict LOF intolerance, particularly in short genes. (biomedcentral.com)
  • In genetics and genomics research, gene expression refers to the process by which information encoded in a gene's DNA sequence is converted into functional proteins or RNA molecules. (pharmiweb.com)
  • To explore work on gene-environment interaction (GxE) at the Centers for Disease Control and Prevention (CDC) and the Agency for Toxic Substances and Disease Registry (ATSDR), we examined the CDC-Authored Genomics and Precision Health Publications Database (CDC-Authored GPHPD) , which includes articles published by CDC/ATSDR authors since 2011. (cdc.gov)
  • How to Do Differential Gene Expression Analysis? (pharmiweb.com)
  • Differential Gene Expression analysis is a fundamental tool for identifying genes whose expression levels significantly differ between experimental conditions or biological samples. (pharmiweb.com)
  • Various statistical tests, such as the negative binomial, edgeR, or DESeq2, are commonly used to assess differential gene expression. (pharmiweb.com)
  • The differentially expressed genes (DEGs) in AD were screened in GSE48350 dataset. (nature.com)
  • The differentially expressed oxidative stress genes (DEOSGs) were analyzed by intersection of DEGs and oxidative stress-related genes. (nature.com)
  • The expression of the following genes was salient: (a) Cd163, an anti-inflammatory stroke-associated gene, was the most differentially expressed gene by NPD1+RvD1, displaying more than a 123-fold upregulation in the ipsilesional penumbra (Lisi et al. (springer.com)
  • In this study, we performed a new bioinformatics analysis on microarray-based gene expression data obtained from the public domain to explore genes that were differentially induced by drugs between the group of capreomycin and PA-824 and the group of mainly the first-line TB drugs. (hindawi.com)
  • In this study, differentially expressed genes (DEGs) under drought and flooding conditions were investigated using Illumina RNA-Seq transcriptome profiling. (frontiersin.org)
  • DEGs, or Differentially Expressed Genes, are genes whose expression levels show significant differences between two or more conditions or experimental groups. (pharmiweb.com)
  • After identifying Differentially Expressed Genes (DEGs), we delve deeper into their functional significance. (pharmiweb.com)
  • These are typically not the most abundant species but far more important are organisms carry copies of distinct, essential genes -- researchers here speak of "keystone genes" -- which are also transcribed disproportionately often. (sciencedaily.com)
  • The ability to accurately predict essential genes intolerant to loss-of-function (LOF) mutations can dramatically improve the identification of disease-associated genes. (biomedcentral.com)
  • Recently, there have been numerous computational methods developed to predict human essential genes from population genomic data. (biomedcentral.com)
  • While the existing methods are highly predictive of essential genes of long length, they have limited power in pinpointing short essential genes due to the sparsity of polymorphisms in the human genome. (biomedcentral.com)
  • Motivated by the premise that population and functional genomic data may provide complementary evidence for gene essentiality, here we present an evolution-based deep learning model, DeepLOF, to predict essential genes in an unsupervised manner. (biomedcentral.com)
  • Unlike previous population genetic methods, DeepLOF utilizes a novel deep learning framework to integrate both population and functional genomic data, allowing us to pinpoint short essential genes that can hardly be predicted from population genomic data alone. (biomedcentral.com)
  • Compared with previous methods, DeepLOF shows unmatched performance in predicting ClinGen haploinsufficient genes, mouse essential genes, and essential genes in human cell lines. (biomedcentral.com)
  • Furthermore, DeepLOF discovers 109 novel essential genes that are too short to be identified by previous methods. (biomedcentral.com)
  • The predictive power of DeepLOF shows that it is a compelling computational method to aid in the discovery of essential genes. (biomedcentral.com)
  • The rationale behind these prediction methods is that essential genes, with minimal inter-individual variation, are subject to purifying selection in order to maintain their sequence in the population, as alterations would lead to reduced fitness. (biomedcentral.com)
  • Introduction to loss of function (LOF) mutations, essential genes versus nonessential genes as well as LOF intolerance versus LOF tolerance. (biomedcentral.com)
  • Enrichment analysis was performed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. (nature.com)
  • We identified the genetic origin of the disorder in the 7 tested families, which happened to be different variants in different genes for each of them. (ub.edu)
  • We identified four different variants putatively affecting splicing patterns of different genes (ASXL1, KAT6A, PIGT and FOXP1) and tested them directly using fibroblasts obtained from patients or indirectly using a mini-gene splicing assay. (ub.edu)
  • Specifically, a long gene typically has a large expected number of LOF variants under a neutral mutation model. (biomedcentral.com)
  • Gene-editing tools are now being used to understand how gene variants are linked to disease in mammalian cells and whole animal models, indicating the potential for this technology to be used to understand and treat human disease (see FIG. 3). (royalsociety.org.nz)
  • Rare Variants in Primary Immunodeficiency Genes and Their Functional Partners in Severe COVID-19. (bvsalud.org)
  • In patients with severe COVID-19, an excess of rare high-impact variants was observed at the whole- exome level, but maximal association signals were detected for PID + FP gene subsets among the genes intolerant to LoF variants, haploinsufficient and essential. (bvsalud.org)
  • Genetic variants with gene regulatory effects are associated with diisocyanate -induced asthma. (cdc.gov)
  • Objective: The aim of this study was to identify DA-associated functional genetic variants through next-generation sequencing (NGS), bioinformatics, and functional assays. (cdc.gov)
  • Four variants exhibited effects on gene regulation (ATF rs11571537, CDH17 rs2446824 and rs2513789, and TACR1 rs2287231). (cdc.gov)
  • These results demonstrate that many DA-associated genetic variants likely act by modulating gene regulation. (cdc.gov)
  • This promoter region polymorphism may be useful as both a functional and an anonymous genetic marker for MAOA. (nih.gov)
  • Among these are "enhancers," sequences that can sit tens of thousands of genetic letters away from a gene, yet still force it into overdrive when activated. (technologynetworks.com)
  • The development of severe COVID-19, which is a complex multisystem disease , is thought to be associated with many genes whose action is modulated by numerous environmental and genetic factors. (bvsalud.org)
  • The focal dermal hypoplasia (FDH) genetic defect has been associated with at least 80 different mutations in the PORCN gene of the X chromosome (Xp11.23). (medscape.com)
  • More than half (23 of 39) of the articles represent epidemiologic studies including candidate gene and genome-wide association studies (GWAS) examining genetic effect modification on disease risk. (cdc.gov)
  • Scientists have now succeeded for the first time in determining key functional genes and the organisms encoding these in such ecological systems, working from extensive data of bacterial genetics and bacterial metabolism. (sciencedaily.com)
  • Scientists of the Luxembourg Centre for Systems Biomedicine (LCSB) and the Life Science Research Unit (LSRU) of the University of Luxembourg, in collaboration with US researchers, have now succeeded for the first time in determining key functional genes and the organisms encoding these in such ecological systems, working from extensive data of bacterial genetics and bacterial metabolism. (sciencedaily.com)
  • Therefore, population genetics-based prediction of LOF-intolerant genes is a powerful strategy to discover haploinsufficient genes associated with human disease. (biomedcentral.com)
  • In this study, we focused on the ideas of the omnigenic model of heritability of complex traits , which assumes that a small number of core genes and a large pool of peripheral genes expressed in disease -relevant tissues contribute to the genetics of complex traits through interconnected networks. (bvsalud.org)
  • They then characterised the entirety of all genes, analysed what genes were being transcribed and gained an overview of the resulting proteins. (sciencedaily.com)
  • This tells us what substances can be produced by the community in general, what genes exist in multiple copies in different bacterial species and in what quantities certain proteins are produced. (sciencedaily.com)
  • Protein-protein, cell signaling, metabolic, and transcriptional interaction networks are useful for identifying connections between lists of experimentally identified genes/proteins. (biomedcentral.com)
  • Studies that utilize genome-wide profiling methods which attempt to explain the differences between two or more experimental conditions such as cells treated with a drug vs. control, diseased tissue vs. normal, gene or protein expression at different time points during cellular differentiation or reprogramming, or candidate gene lists harboring mutations associated with a particular disease, produce lists of genes/proteins without apparent functional relationship. (biomedcentral.com)
  • Calculating the similarity between gene expression patterns (co-expression degree) by using gene expression data has an important guiding function in understanding the relationship between the corresponding proteins of the gene, and can help to identify whether different proteins have same or similar functions and whether they can be composed as protein complexes or functional modules. (biomedcentral.com)
  • Multiple gene mutations have been identified and considered as important substrates for the development of MDS, such as RNA splicing, histone manipulation, DNA methylation, transcription factors, kinase signaling, DNA repair, cohesin proteins, and other signal transduction elements. (frontiersin.org)
  • The sodium channels containing proteins produced from the SCN5A gene are abundant in heart (cardiac) muscle cells and play key roles in these cells' ability to generate and transmit electrical signals. (medlineplus.gov)
  • This gene provides instructions for making a protein that is responsible for modifying other proteins, including Wnt proteins. (medscape.com)
  • PORCN , a member of the porcupine (PORC) gene family, encodes transmembrane endoplasmic reticulum proteins that target Wnt signaling proteins. (medscape.com)
  • Drosophila melanogaster porcupine and its mouse homologue PORCN gene encode transmembrane bound endoplasmic reticulum proteins needed for the secretion of Wnt (Wingless and INT-1) proteins. (medscape.com)
  • From gene expression knockdown with CRISPRi and RNAi to overexpression with CRISPRa and ORF, we have everything you need for your target discovery research. (sigmaaldrich.com)
  • Toxicogenomics tries to understand and predict drug toxicity by studying gene expression. (cam.ac.uk)
  • However, besides physical or co-expression interactions there are many ways in which pairs of genes, or their protein products, can be associated. (biomedcentral.com)
  • Developing a protein function module recognition method ECTG based on Topological Features and Gene expression data for Protein Complex Identification. (biomedcentral.com)
  • The algorithm can effectively remove the noise data reflected by calculating the topological structure characteristic values in the PPI network through the similarity of gene expression patterns, and also properly use the information hidden in the gene expression data. (biomedcentral.com)
  • ECTG(Evolutionary Clustering Algorithm Based on Topological Features and Gene expression data for Protein Complex Identification). (biomedcentral.com)
  • This method is based on evolutionary algorithm (EA), which effectively fuses protein topology and gene expression data. (biomedcentral.com)
  • The immune-related DEOSGs and hub genes were identified by weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis, respectively. (nature.com)
  • The mRNA expression of diagnostic genes was determined by qRT-PCR analysis. (nature.com)
  • Oxidative stress is involved in the modulation of gene expression patterns and metabolic activities, characterized by the disruption of redox homeostasis 7 . (nature.com)
  • The sequencing of single oocytes and their surrounding somatic cells revealed coordinated expression of hundreds of genes that formed functional regulatory networks. (auburn.edu)
  • Here, we examined the expression profile of 108 patients with MDS from the GSE58831 dataset, and identified 22 genes that were significantly associated with overall survival. (frontiersin.org)
  • However, with the development of gene expression profile and new high-throughput technology, the understanding of the pathogenesis of MDS is getting further and better. (frontiersin.org)
  • Genes whose expression levels rise and fall similarly in a large set of samples, may be considered coexpressed. (mdpi.com)
  • We also performed a transcriptomics analysis on fibroblasts from different patients carrying TRAF7 mutations, which showed alterations in the expression of different genes that might contribute to the phenotype. (ub.edu)
  • therefore, it is imperative to assess the expression of genes involved in response to flood and drought stress. (frontiersin.org)
  • The expression of photosynthesis and chlorophyll synthesis related genes were significantly reduced under both types of stresses, which limit the metabolic processes and thus help prolong survival under extreme conditions. (frontiersin.org)
  • The changes in expression of genes involved in regulating the flux of cell wall precursors and starch/sugar content can serve as an adaptive mechanism for soybean survival under stress conditions. (frontiersin.org)
  • for example, Chu and Chen [ 13 ] combined PPI and gene expression data to construct a cancer perturbed PPI network in cervical carcinoma to study gain- and loss-of-function genes as potential drug targets. (hindawi.com)
  • 14 ] combined PPI and gene expression data to construct tissue-specific PPI networks for 60 tissues and used them to prioritize disease genes. (hindawi.com)
  • When studying gene expression, researchers often compare gene expression profiles between different biological samples or experimental conditions, such as healthy and diseased tissues, treated and untreated cells, or different developmental stages. (pharmiweb.com)
  • By analyzing the expression levels of thousands of genes simultaneously, researchers can identify genes that are upregulated (higher expression) or downregulated (lower expression) in one condition compared to another. (pharmiweb.com)
  • With RNA-seq , we can capture and quantify the abundance of transcripts, revealing the dynamic landscape of gene expression within a cell or tissue. (pharmiweb.com)
  • Once the reads are mapped, we quantify the expression level of each gene. (pharmiweb.com)
  • Statistical methods are employed to identify genes that exhibit significant changes in expression between experimental conditions. (pharmiweb.com)
  • By comparing gene expression patterns during embryogenesis or tissue differentiation, we unravel the molecular events that shape organisms, advancing our understanding of developmental biology. (pharmiweb.com)
  • In the luciferase assay 4 of the 21 SNPs exhibited allele-dependent changes in gene expression. (cdc.gov)
  • Results: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana , including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. (lu.se)
  • We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. (lu.se)
  • Although biochemical functions of the human PORCN gene are not well characterized, Wnt signaling may be involved in the phenotypic expression of focal dermal hypoplasia where defective/deficient Wnt signaling could affect cell fate or result in failure of a progenitor cell line to expand. (medscape.com)
  • Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)
  • 0 gene-expression profiling to tinct subtypes of SRBCTs (Fig. 1). (lu.se)
  • Gene-expression patterns used to differentiate four type cause heart damage, and markable finding that genes cur- types of SRBCT. (lu.se)
  • In a general gene expression. (lu.se)
  • In a general gene expression pattern screen for panarthropod Fox genes including the red flour beetle Tribolium castaneum, the pill millipede Glomeris marginata, the common house spider Parasteatoda tepidariorum, and the velvet worm Euperipatoides kanangrensis, we identified a Fox gene with a highly conserved expression pattern along the ventral ectoderm of arthropod and onychophoran limbs. (lu.se)
  • How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)
  • Interleukin-8 (IL-8) gene polymorphisms have been considered as susceptibility factors in periodontal disease. (nih.gov)
  • The Ahl gene appears to play a role in susceptibility to NIHL but, other genes as well as systemic and local factors must also be involved. (cdc.gov)
  • While it is relatively straightforward to identify LOF mutations in protein-coding genes, it is challenging to infer their effects on evolutionary fitness and disease risk. (biomedcentral.com)
  • A promising high throughput strategy, which is the gene-annotation enrichment analysis, can increase the elucidation ability of related biological processes. (eurekaselect.com)
  • As an exclusive provider of the TRC1.5 and TRC2 collections from The RNAi Consortium (TRC), our comprehensive offering consists of nearly 200,000 pre-cloned shRNA constructs targeting more than 22,500 human and 20,000 mouse genes. (sigmaaldrich.com)
  • Genes2FANs is a web based tool and a database that utilizes 14 carefully constructed FANs and a large-scale protein-protein interaction (PPI) network to build subnetworks that connect lists of human and mouse genes. (biomedcentral.com)
  • The FANs are created from mammalian gene set libraries where mouse genes are converted to their human orthologs. (biomedcentral.com)
  • This section includes genomic Reference Sequences (RefSeqs) from all assemblies on which this gene is annotated, such as RefSeqs for chromosomes and scaffolds (contigs) from both reference and alternate assemblies. (nih.gov)
  • The long-term undifferentiation of molluscan sex chromosomes is potentially sustained by the unexpected intertwined regulation of reversible sex-biased genes, together with the lack of sexual dimorphism and occasional sex chromosome turnover. (nature.com)
  • The pleiotropic constraint of regulation of reversible sex-biased genes is widely present in ancient homomorphic sex chromosomes and might be resolved in heteromorphic sex chromosomes through gene duplication followed by subfunctionalization. (nature.com)
  • The evolutionary dynamics of sex chromosomes suggest a mechanism for 'inheritance' turnover of sex-determining genes that is mediated by translocation of a sex-determining enhancer. (nature.com)
  • This approach also abstracts the genes from the query list to higher order biological functions, allowing for the identification of novel relevant genes. (biomedcentral.com)
  • DGE analysis facilitates the identification of genes responsive to specific drug treatments. (pharmiweb.com)
  • ABSTRACT This study aimed to evaluate the association of plasma MIF level and -173 G/C single nucleotide polymorphism of the MIF gene with the occurrence, severity and mortality of sepsis patients. (who.int)
  • To control these shifting patterns, our genomes contain regulatory sequences that turn genes on and off in response to specific chemical cues. (technologynetworks.com)
  • The genomes of malaria parasites contain many genes of unknown function. (gla.ac.uk)
  • Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench. (sigmaaldrich.com)
  • Here, we demonstrate for the first time the use of the CRISPR/Cas9 system to engineer the IL-8 gene, and tested the functionality of different haplotypes. (nih.gov)
  • Now a research team led by University of California and Chan Zuckerberg Biohub scientists has used a modified version of the gene-editing technique CRISPR to find enhancers -- not by editing them but by prompting them into action. (technologynetworks.com)
  • A team from UC San Francisco and the University of California, Berkeley, used a tool called CRISPR activation (CRISPRa), developed at UCSF in 2013, to search for enhancers of a gene that affects development of the immune cells known as T cells. (technologynetworks.com)
  • Now we are using a modified version of the gene-editing technique CRISPR to find enhancers - not by editing them but by prompting them into action with a tool called CRISPR activation (CRISPRa), developed at UCSF in 2013. (technologynetworks.com)
  • For example, CRISPR has been used in research mouse models to correct a mutation in genes responsible for Hepatitis B, haemophilia, severe combined immunodeficiency, cataracts, cystic fibrosis, hereditary tyrosinemia and inherited Duchenne muscular dystrophy. (royalsociety.org.nz)
  • Researchers at Deakin University in Australia are working with gene modifications using CRISPR to produce hypoallergenic eggs. (royalsociety.org.nz)
  • In the UK, the Human Fertilisation and Embryology Authority (HFEA) has approved an application for the use of CRISPR in healthy human embryos to help researchers to investigate the genes involved in early embryo development. (royalsociety.org.nz)
  • In China, researchers have used CRISPR in non-viable human embryos to genetically modify genes responsible for ß-thalassemia, a potentially fatal blood disorder, and to modify genes in immune cells to develop increased HIV resistance. (royalsociety.org.nz)
  • In commercial development, the common white button mushroom has been modified by CRISPR at Penn State University to prevent them from becoming off-colour by targeting a gene that produces an enzyme that causes browning. (royalsociety.org.nz)
  • We hypothesized that the transcript profiles of the cumulus cells and oocyte display distinct gene regulatory networks within the oocyte and cumulus cells. (auburn.edu)
  • Forkhead box (Fox) transcription factors evolved early in animal evolution and represent important components of conserved gene regulatory networks (GRNs) during animal development. (lu.se)
  • ATC/TTC haplotype cells significantly increased transmigration of neutrophils confirming the functional role for this IL-8 haplotype. (nih.gov)
  • A significantly higher diagnosis yield was reached compared with other studies, potentially explained by a deep analysis of the sequencing data using in silico predictors, followed by the performance of specific functional studies for each case. (ub.edu)
  • Notably, at a false positive rate of 5%, DeepLOF detects 50% more ClinGen haploinsufficient genes than previous methods. (biomedcentral.com)
  • It is these patterns of expressed and repressed genes that determine what kind of cell -- kidney, brain, skin, heart -- each will become. (technologynetworks.com)
  • 0 pression patterns of 96 transcripts and prognosis based on a limited representing 80 known genes and 20 13 numbers of markers1-5. (lu.se)
  • Of the approximately 25,000 identified genes in the human genome so far, mutations in over 3,000 have been linked to disease. (royalsociety.org.nz)
  • Determining which genes are LOF intolerant can aid with discovery of human disease genes. (biomedcentral.com)
  • We describe a new polymorphism upstream of the gene for monoamine oxidase A (MAOA), an important enzyme in human physiology and behavior. (nih.gov)
  • The polymorphism is in linkage disequilibrium with other MAOA and MAOB gene markers and displays significant variations in allele frequencies across ethnic groups. (nih.gov)
  • The polymorphism has been shown to affect the transcriptional activity of the MAOA gene promoter by gene fusion and transfection experiments involving three different cell types. (nih.gov)
  • Functional Polymorphism in the ADRB3 Gene, Encoding the Beta-3 Adrenergic Receptor, and Response to Intra-Detrusor Injection of Botulinum Toxin-A in Women with Overactive Bladder. (urotoday.com)
  • There are reports suggesting an association between the rs4994 polymorphism in the ADRB3 gene encoding the beta-3 adrenergic receptor and OAB risk in females. (urotoday.com)
  • High MIF levels and MIF -173G/C gene polymorphism are powerful predictors of the severity of sepsis and its outcome. (who.int)
  • Investigation of hub genes for the development of potential therapeutic targets and candidate biomarkers is warranted. (nature.com)
  • DMY is the second vertebrate sex-determining gene identified from the fish, Oryzias latipes. (amrita.edu)
  • We showed that sarcolamban, encoding two smORFs of 28 and 29aa, is the true homologue of the vertebrate genes sarcolipin and phospholamban. (cabd.es)
  • Two sgRNAs vectors targeting the IL-8 gene and the naked homologous repair DNA carrying different haplotypes were used to successfully generate HEK293T cells carrying the AT genotype at the first SNP - rs4073 (alias -251), TT genotype at the second SNP - rs2227307 (alias +396), TC or CC genotypes at the third SNP - rs2227306 (alias +781) at the IL-8 locus. (nih.gov)
  • Sequencing of a 2.5-kb chromosomal Sma I- Pst I fragment containing the structural gene revealed an open reading frame encoding 537 amino acids, homologous to known pckA genes. (asm.org)
  • The three define an ancient gene family and their peptides are structurally and functionally homologous. (cabd.es)
  • We will explore the options for antibiotic resistance gene annotations, showing participant how they can broadly annotate hundreds of antibiotic resistance genes from different data input and perform detailed BLAST analysis in CARD, Resfam and Pfam. (dtu.dk)
  • Using a human liver cell culture model, SimuGen has demonstrated that with the correct choice of biomarkers, empiric functional data analysis models, and higher level exploratory analysis such as clustering (based on the models), it is possible to predict and describe liver toxicity with greater sensitivity than animal tests. (cam.ac.uk)
  • The diagnostic value of hub genes was assessed by receiver operating characteristic analysis and validated in GSE1297. (nature.com)
  • We obtained 66 immune-related DEOSGs by intersecting turquoise module with the DEOSGs and identified 15 hub genes through PPI analysis. (nature.com)
  • Gene coexpression analysis refers to the en masse discovery of coexpressed genes from a large variety of transcriptomic experiments. (mdpi.com)
  • The emergence of high throughput data in genomic, proteomic and bioinformatics has increased the necessity to develop large amount of functional analysis tools to facilitate the inference of biologically meaningful data. (eurekaselect.com)
  • Gene reduction analysis revealed that the gene deletion processes are under selective pressure, and many of the inactivations are probably related to the organism's interaction with its host environment. (asm.org)
  • This analysis enables us to pinpoint genes that play pivotal roles in phenotypic variation, disease development, or response to treatments. (pharmiweb.com)
  • DGE analysis elucidates how genes respond to environmental stimuli, such as heat stress, chemical exposure, or pathogen infection. (pharmiweb.com)
  • Reuters) - The U.S. Food and Drug Administration rejected BioMarin Pharmaceutical Inc's gene therapy for bleeding disorder hemophilia A citing the need for longer-term data, the drugmaker said on Wednesday, potentially pushing any approval out to 2022. (equities.com)
  • From these, we can for example identify key functional traits within the community and we are then able to attribute such traits to specific community members. (sciencedaily.com)
  • JR: Why was there a need for a technique that can identify stimulus-responsive enhancers for a target gene, independent of exposure to the stimulus? (technologynetworks.com)
  • This workshop introduces the functional metagenomic selections as a tool to identify known and novel antibiotic resistance gene from complex clinical and environmental communities. (dtu.dk)
  • By systematically incorporating knowledge on shared properties of genes from diverse sources to build functional association networks (FANs), researchers may be able to identify additional functional interactions between groups of genes that are not readily apparent. (biomedcentral.com)
  • To identify the photoreceptive molecule that regulates photoperiodic responses, in the present study, we have cloned and characterized the cDNA encoding an opsin gene expressed in the ayu brain, a putative site of the photoreceptor for photoperiodism. (bioone.org)
  • The authors used artificial neural croarrays is to study changes in gene ex- .nature organ the tumor arose from, how big it networks (ANNs) to identify gene-ex- pression that accompany changes in is, and what it looks like after it has pression signatures associated with spe- cell physiology, such as during develop- been imbedded in wax and colored cific subtypes of tumors. (lu.se)
  • In this thesis, we have used a combination of whole-exome sequencing (WES) and functional studies to establish the diagnosis of 9 cases tentatively diagnosed as Opitz C, a clinical entity that encompasses patients with very different molecular causes. (ub.edu)
  • Illumina and 454 data and Sanger resequencing yielded circularized plastomes of 11,348 and 15,167 bp length, with both species containing five possibly functional genes (accD, rps3, rps4, rm16, rm23) and two/three pseudogenes (rpoC2 in P. aethiopica and rpf2 and rps12 in both species;rps12 may be functional in P. hamiltonii). (uni-muenchen.de)
  • Functional data from each mouse were aligned with the cytocochleogram using the frequency-place map of Ou et al. (cdc.gov)
  • Better integration of GWAS with functional data? (who.int)
  • Armed with this knowledge, the researchers then went about first identifying "keystone genes" and linking these to keystone species. (sciencedaily.com)
  • Gene editing is also being used by researchers to try to overcome allergic reactions to chicken eggs, which prevents about 2% of children worldwide from receiving many routine vaccinations. (royalsociety.org.nz)
  • In the US, researchers have used gene-editing technologies on agricultural crops such as maize, soybean, sorghum, and developed a rice resistant to bacterial blight. (royalsociety.org.nz)
  • Finally, we constructed the drug, transcription factors (TFs), and microRNA network of the diagnostic genes. (nature.com)
  • These genes comprise 289 Transcription Factors (TFs) representing Basic Helix-loop Helix (bHLH), Ethylene Response Factors (ERFs), myeloblastosis (MYB), No apical meristem (NAC), and WRKY amino acid motif (WRKY) type major families known to be involved in the mechanism of stress tolerance. (frontiersin.org)
  • Translation may be a secondary consequence of a non-coding function (for example, in the regulation of translation of other sequences) and give raise to non-functional peptides. (cabd.es)
  • Independent clustering of co-expressed genes revealed critical biological functions for the oocyte, such as regulation of transcription, stem cell population maintenance, and insulin receptor signaling pathway. (auburn.edu)
  • In cumulus cells translation, cellular response to insulin stimulus, and regulation of transcription emerged as critical functions among co-expressing genes. (auburn.edu)
  • Functional investigation of FoxB in Parasteatoda reveals a hitherto unrecognized important function of FoxB upstream of wingless (wg) and decapentaplegic (dpp) in the GRN orchestrating dorsal-ventral limb patterning. (lu.se)
  • Tissues in which cells select for the defective PORCN gene show anomalies. (medscape.com)
  • Some SCN5A gene mutations associated with Brugada syndrome change single protein building blocks (amino acids) in the SCN5A protein. (medlineplus.gov)
  • The SCN5A gene mutations change single amino acids in the SCN5A protein. (medlineplus.gov)
  • The SCN5A gene mutations that cause Romano-Ward syndrome include changes in single amino acids and deletions or insertions of a small number of amino acids in the SCN5A protein. (medlineplus.gov)
  • Aiming to characterize truncating mutations in MAGEL2, which are responsible for Schaaf-Yang syndrome (SYS), we performed different experiments that suggest a potential toxic effect of the produced truncated form of the protein, which lacks its most relevant functional domain. (ub.edu)
  • The T4 Gene 32 Protein is intended for molecular biology applications. (qiagen.com)
  • Keystone species: Which are most important functional genes in an ecosystem? (sciencedaily.com)
  • With this knowledge about keystone genes and species, we can take a closer look at the molecular causes in future -- and explore measures for restoring ecological balance in the gut, for example. (sciencedaily.com)
  • The 23 species of mycoheterotrophic or exoparasitic land plants (from 15 genera and 6 families)studied so far all retain a minimal set of 17 of the normally 116 plastome genes. (uni-muenchen.de)
  • The genome assemblies and functional annotations of scallop species are also available in the MolluscDB database ( http://mgbase.qnlm.ac/page/download/download ). (nature.com)
  • Although arthropods represent the largest and most diverse animal group, most other Fox genes have not been investigated in detail, not even in the arthropod model species Drosophila melanogaster. (lu.se)
  • In plant populations, self-incompatibility mechanisms may cause related plant specimens to be incompatible, which may lead to functional extinction if an entire population becomes self-incompatible. (wikipedia.org)
  • SCN5A gene mutations also cause sudden unexpected nocturnal death syndrome (SUNDS), which was originally described in Southeast Asian populations. (medlineplus.gov)
  • For example, studies suggest that ccRCC is closely associated with tumor suppressor von-Hippel Lindau ( VHL ) gene mutations that lead to stabilization of hypoxia inducible factors (HIF-1 α and HIF-2 α , also known as HIF1A and EPAS1) in both sporadic and familial forms [ 5 , 6 ]. (hindawi.com)
  • Studies indicate that focal dermal hypoplasia (FDH) is usually caused by mutations of the PORCN gene, mapped to locus Xp11.23. (medscape.com)
  • To test whether ATC/TTC haplotype is functional, we used a trans-well assay to measure the transmigration of primary neutrophils incubated with supernatants from the Poly I:C stimulation experiment. (nih.gov)
  • DNA binding of single stranded DNA by T4 Gene 32 Protein was measured using a gel shift assay with a single-stranded, fluorescently labeled oligonucleotide. (qiagen.com)
  • Birth defects can be defined as structural or functional abnormalities, including metabolic disorders, which are present from birth. (who.int)
  • However, the effect of autophagy-related genes (ARGs) on the prognosis of MDS remains unclear. (frontiersin.org)
  • This can be achieved by counting the number of reads that align to each gene or by estimating transcript abundance using sophisticated algorithms. (pharmiweb.com)
  • Our study identified 9 key genes associated with oxidative stress and immune reaction in AD pathogenesis. (nature.com)