Gene from mice. Medical search. Frequent questions

According to the researchers, gene transfer into specific cell lineages in vivo remains an attractive yet elusive approach for correcting inherited mutations. (sciencedaily.com)
Mutations in the beta subunit of the cGMP phosphodiesterase gene (beta PDE) can cause a recessively inherited retinal degeneration in several species, including mice, dogs and humans. (nih.gov)
Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Mice homozygous for targeted mutations in this gene are viable, fertile and phenotypically normal. (jax.org)
Retinitis pigmentosa, estimated to impair the vision of one in 4,000 people, can be caused by mutations in over 100 different genes. (scienceboard.net)
To restore vision in the mice, the team developed a new and more versatile CRISPR system called PE SpRY , which can be programmed to correct many different genetic mutations, regardless of where in the genome they occur. (scienceboard.net)
One such molecule, known as KITL, controls numerous stages of oocyte development, which is why individuals harboring mutations within the gene that codes for this particular compound tend to suffer from fertility issues . (iflscience.com)
As CRISPR-Cas9, a new gene-editing technology, moves into gene therapy clinical trials, a new study published in Nature Methods has reported that it can introduce hundreds of unintended mutations into the genome of mice, including single nucleotide mutations and mutations in non-coding regions of the genome (Item 1). (biosafety-info.net)
In the new study, the researchers sequenced the entire genome of mice that had undergone CRISPR gene editing in the team's previous study and looked for all mutations, including those that only altered a single nucleotide. (biosafety-info.net)
The researchers found that the genomes of two independent gene therapy recipients had sustained more than 1,500 single-nucleotide mutations and more than 100 larger deletions and insertions. (biosafety-info.net)
Dr Michael Antoniou, a London-based molecular geneticist, believes that not only is it necessary to conduct whole genome sequencing to identify all off-target mutations from CRISPR-based genome editing, but it is also essential to ascertain the effects of these unintended changes on global patterns of gene function (Item 2). (biosafety-info.net)
New York- As CRISPR-Cas9 starts to move into clinical trials, a new study published in Nature Methods has found that the gene-editing technology can introduce hundreds of unintended mutations into the genome. (biosafety-info.net)
Mutations affecting the expression of genes are known to underlie much of adaptive evolution. (biorxiv.org)
Such mutations can arise in cis -(near the gene of interest) or in trans -(at a distant locus), but the relative contribution of these different kinds of changes to adaptation is poorly understood, especially during very recent divergence. (biorxiv.org)
Using crosses between warm-adapted and cold-adapted mice, we found that mutations acting in a context specific manner ( cis -regulatory and tissue-specific changes) predominate expression divergence and are more likely to be involved in parallel evolution. (biorxiv.org)
This protocol is significant for creating and determining mind-body desired mutations in the genome of Pseudomonas aeruginosa, as well as the testing the effect of mutations on virulence reduction in a reproducible mouse model. (jove.com)
Many variants (also called mutations) have been identified in the ARG1 gene. (medlineplus.gov)
Loss of function mutations in conserved regions of the human arginase I gene. (medlineplus.gov)

The mHTT and wtHTT groups showed the highest number of differentially expressed genes compared to the BACHD mouse model. (lu.se)

The following LCE1M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
This figure increases to 87% using less stringent criteria for gene structure similarity, thus implying that for about 13% of the human RefSeq annotated genes (and about 25% of the corresponding transcripts) we could not identify any mouse transcript showing sufficient similarity to be confidently assigned as a splicing ortholog. (biomedcentral.com)

The C57BL/6J (B6) and DBA/2J (D2) inbred strains of mice exhibit an age-related hearing loss (AHL) due to a recessive gene (Ahl) that maps to Chromosome 10. (cdc.gov)

Researchers have discovered a new gene therapy that may prevent the progression of emphysema. (sciencedaily.com)
Using mice, the BUSM researchers discovered a system to deliver genes selectively to as many as 70 percent of a mouse lung's alveolar macrophages (AM), a key cell type contributing to emphysema. (sciencedaily.com)
According to the researchers after 24 weeks of sustained gene expression, no humoral or cellular immune responses to the human hAAT protein were detected. (sciencedaily.com)
Going into the study, the researchers worried the muscle growth catalyzed by the gene therapy could harm the heart, mainly through thickening of the heart's walls. (singularityhub.com)
The antibiotics' beneficial effects, discovered in experiments in the lab and with mice, are unrelated to their ability to kill bacteria, the researchers report in the Jan. 6 issue of Nature. (scienceblog.com)
Of the antibiotics, penicillin protected nerve cells best in laboratory dishes, but ceftriaxone had the best results in mice, probably because it more easily crosses into the brain from the blood, the researchers report. (scienceblog.com)
In a series of experiments, the researchers discovered that the antibiotics activate the gene encoding glutamate's main transporter in brain cells. (scienceblog.com)
Researchers have assigned phenotypes to around 150 mouse genes, according to a study published in Nature Genetics this week (July 27). (the-scientist.com)
The researchers examined more than 27,000 mice to categorize the phenotypes the mice developed as a result. (the-scientist.com)
March 17, 2023 -- Researchers in China successfully restored the vision of mice with retinitis pigmentosa, a major cause of blindness in humans. (scienceboard.net)
The researchers also performed a variety of behavioral tests, which confirmed that the gene-edited mice retained their vision even into old age. (scienceboard.net)
Researchers at the Francis Crick Institute have developed a database of gene activity in mice, which could reduce the use of animals in medical research. (verdict.co.uk)
Although the ethics of animal testing may be hotly debated, currently, when studying a particular disease, researchers create, infect, cull, obtain samples from mice and extract and sequence the RNA. (verdict.co.uk)
Accessible via an app, researchers will be able to view the activity of any gene across a range of diseases without needing their own mice. (verdict.co.uk)
Using sequencing technology, researchers measured gene activity across the different diseases and analysed the genetic signatures associated with these diseases to help understand the immune response. (verdict.co.uk)
Researchers anywhere in the world can now view this information on the gene activity in the lungs and blood of mice infected with a range of pathogens including the , influenza virus, the respiratory syncytial virus, and the house dust mite allergen. (verdict.co.uk)
This is the result of a ten-year project, with researchers first used a technique called microarray back in 2009 to detect gene activity in lung and blood samples. (verdict.co.uk)
A team of researchers, including UConn assistant professor of pharmaceutics Raman Bahal , has, for the first time, corrected a genetic mutation in a mammalian fetus using a targeted gene editing technique. (uconn.edu)
In the current study, the researchers injected the nanoparticle suspension into the amniotic fluid of pregnant mice whose fetuses carried a genetic mutation that causes beta thalassemia, a blood disorder that reduces the production of hemoglobin and causes a severe form of anemia. (uconn.edu)
Untreated mice with the genetic disorder died much earlier, the researchers found. (uconn.edu)
Most importantly, the researchers say, the chemically-oriented process resulted in no off-target effects from treatment, which has been a major concern for other gene editing tools like CRISPR/Cas9, which can erroneously damage untargeted DNA and cause complications. (uconn.edu)
A crucial clue to explain the DFNA27 form of progressive deafness arose from later studies of the mouse RE1 Silencing Transcription Factor, or Rest, gene conducted by researchers at the University of Iowa. (hearingreview.com)
Because the human counterpart of the mouse Rest gene is located in the DFNA27 region, the Iowa and NIDCD researchers teamed up to reexamine the mystery of DFNA27 progressive deafness. (hearingreview.com)
While the researchers hoped to see an increase in KITL production within these infertile mice, they were somewhat stunned to find that the rodents actually became capable of reproduction following treatment. (iflscience.com)
NewsNation ) - Researchers at the University of Rochester transferred a longevity gene from naked mole rats to mice, calling it a "groundbreaking endeavor. (wgnradio.com)

A European consortium identifies phenotypes for 320 genes, assigning new functions for half. (the-scientist.com)
cis -regulated genes under selection influenced phenotypes such as body size, fat content, immune functioning, and skeletal morphology. (biorxiv.org)

The study describes a method to express therapeutic genes in lung tissue for a lifetime after only a single treatment. (sciencedaily.com)
Results We investigated the alteration of genome-wide transcription in mouse skeletal muscle tissue (rectus femoris muscle) during aging using a high-throughput sequencing technique. (figshare.com)
Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells. (genscript.com)
Cartilage thickness and proteoglycan staining of articular cartilage in the knee joints of 12-week-old KO mice were increased as compared to controls, with higher cellularity throughout the tissue. (bepress.com)
Here, we studied gene expression in liver and brown adipose tissue in two wild-derived strains of house mice that independently adapted to cold, northern environments, and we compared them to a strain of house mice from a warm, southern environment. (biorxiv.org)
Second, we discovered that expression evolution occurred largely at tissue-specific and cis -regulated genes, and that these genes were over-represented in parallel cases of evolution. (biorxiv.org)
These results demonstrate that parallel patterns of gene expression in mice that have independently adapted to cold environments are driven largely by tissue-specific and cis -regulatory changes, providing insight into the mechanisms of adaptive gene regulatory evolution at the earliest stages of divergence. (biorxiv.org)
Excel file with five tabs, providing (i) the legends for the tissue sources, (ii) the table for the gene lists, (iii) the information for the reassembled transcripts, (iv) the proteomic evidence shown in detail, and (v) the accession numbers for the ENCODE data. (elifesciences.org)

Interestingly, insulin injection of wild-type mice, which activates PKB (protein kinase B) and inhibits GSK3 to a greater degree than feeding (50% versus 25%), does not repress these genes. (portlandpress.com)
The number of stimulations required to evoke focal (grade 1 and 2) seizures did not differ between the groups, but the NT-3 mutants spent a considerably longer period of time (13 +/- 3 days) than wild-type mice (2 +/- 1 days) in grade 2 seizures. (lu.se)
BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)

Alpha-1 Anti-trypsin Deficiency is the most common inherited form of emphysema seen in young people due to a mutation in the Alpha-1 Anti-trypsin gene. (sciencedaily.com)
They used mice with retinitis pigmentosa caused by a mutation in the PDE6β gene, which encodes a critical enzyme. (scienceboard.net)
When programmed to target the mutant PDE6β gene, the PE SpRY system was able to correct the mutation and restore the enzyme's activity in the retinas of the affected mice. (scienceboard.net)
But mice who had the PDE6β gene mutation corrected through the PE SpRY system had much thicker retinas containing numerous rod cells. (scienceboard.net)
Yet in a paper appearing in the international science journal Nature Communications , scientists from UConn, Yale University, and Carnegie Mellon University describe how they corrected a genetic mutation in fetal mice using an intravenous injection of nanoparticles loaded with a combination of donor DNA and synthetic molecules known as peptide nucleic acids or PNAs. (uconn.edu)
Almkvist O, Basun H, Wagner SL, Rowe BA, Wahlund LO, Lannfelt L (1997) Cerebrospinal fluid levels of alpha-secretase-cleaved soluble amyloid precursor protein mirror cognition in a Swedish family with Alzheimer disease and a gene mutation. (springer.com)
The investigators localized the deafness-causing mutation to a region on chromosome four called DFNA27 , which includes a dozen or so genes. (hearingreview.com)

Overexpression of GSK3 in cells antagonizes insulin regulation of these genes. (portlandpress.com)
In the present study we demonstrate that regulation of these three genes by feeding is normal in mice that express insulin-insensitive GSK3. (portlandpress.com)
These results indicate that endogenous NT-3 levels can influence the rate of epileptogenesis, and suggest a link between NT-3 and BDNF gene regulation in dentate granule cells. (lu.se)
Botond Banfi, MD, PhD and Yoko Nakano, PhD, lead authors of the current study, discovered that mouse Rest is regulated through an unusual mechanism in the sensory cells of the inner ear, and this regulation is critical for hearing in mice. (hearingreview.com)
Changes in gene regulation have long been appreciated as a driving force of adaptive evolution, however the relative contributions of cis- and trans- acting changes to gene regulation over short evolutionary timescales remain unclear. (biorxiv.org)
Instances of recent, parallel phenotypic evolution provide an opportunity to assess whether parallel patterns are seen at the level of gene expression, and to assess the relative contribution of cis - and trans -changes to gene regulation in the early stages of divergence. (biorxiv.org)

Oct. 24, 2023 One problem in gene therapy is that not all genes transfer equally well into the target cells. (sciencedaily.com)

Mus musculus predicted gene, 21698 (Gm21698), mRNA. (genscript.com)

The aim of this study was to identify the genes and pathways underlying the growth of the mouse sclera during postnatal development. (molvis.org)
In AAV groups, from the combined limma with GSEA using ClusterProfiler, we found both shared and unique gene sets and pathways for mice with wtHTT overexpression compared to mice with mHTT overexpression. (lu.se)
Multiple biosynthetic pathways such as sterol synthesis were among the top shared processes, where both unique and shared genes constituted leading-edge subsets. (lu.se)
In conclusion, mice with targeted overexpression of HTT (wtHTT or mHTT) in the hypothalamus show dysregulation of pathways, of which there are subsets of shared pathways and pathways unique to either wtHTT or mHTT overexpression. (lu.se)

In situ hybridization showed 30% reduction of basal NT-3 mRNA levels and lack of upregulation of TrkC mRNA expression at 2 h after a generalized seizure in dentate granule cells of the NT-3+/- mice, whereas the seizure-evoked increase in brain-derived neurotrophic factor (BDNF) and TrkB mRNA levels was enhanced. (lu.se)
In general, mRNA levels of ECM and collagen genes have been found to be downregulated in both the adult mouse sclera [ 6 ] and cornea [ 7 ] when compared with samples from an earlier developmental stage. (molvis.org)
Most previous studies had missed exon 4 in the Rest gene because this small exon is not edited into the Rest mRNA in most cells. (hearingreview.com)
Interestingly, prior intraperitoneal administration (15 min) of CINN (0.5 mM) significantly reduced cardiac lactate concentration, activities of HK and CS, and mRNA levels of PGC-1α and COX-IV in mice that ran at 90% of Smax. (bvsalud.org)

Here, based on significant liver contribution to the catabolism of the branched chain amino acid leucine, we develop a liver-directed adeno-associated virus (AAV8) gene therapy for MSUD. (nature.com)
The study authors, therefore, used a type of virus called an adeno-associated virus (AAV) to introduce a gene for the production of KITL into female mice that lacked this key reproductive molecule. (iflscience.com)

We tested the possibility of altering the course of retinal degeneration in the rd mouse through subretinal injection of a recombinant replication-defective adenovirus that contains the murine cDNA for wild-type (beta PDE, Ad.CMV beta PDE. (nih.gov)
Total RNA was isolated from each of 30 single mouse sclera (n=30, 6 sclera each from 1-, 2-, 3-, 6-, and 8-week-old mice) and reverse-transcribed into cDNA using a T7-N 6 primer. (molvis.org)
The resulting cDNA was fragmented, labeled with biotin, and hybridized to a Mouse Gene 1.0 ST Array. (molvis.org)

Gene Set Enrichment Analysis (GSEA) with leading-edge analysis showed that suppressed sterol- and cholesterol metabolism were shared between hypothalamic wtHTT and mHTT overexpression. (lu.se)
To analyze microarray datasets (34760 variables) and obtain functional implications of differential expression patterns, we used Linear Models for Microarray Data (limma) followed by Gene Set Enrichment Analysis (GSEA) using ClusterProfiler. (lu.se)

Precision genome editing agents can enable gene correction and disease rescue in inherited retinal diseases, according to University of California, Irvine. (scienceboard.net)
Esvelt says that the feedback is still coming in, but the initial reaction from Nantucket is pushing the project toward aiming to develop mice resistant not just to Lyme disease but to other tick-borne diseases , like babesiosis. (wbur.org)
This study provides a benchmark for gene editing technology where diseases can be treated at a very early stage of development," says Bahal, a recent arrival to UConn who performed the research when he was an associate scientist working in the Yale lab of Dr. Peter Glazer, a professor of therapeutic radiology and genetics. (uconn.edu)
Gene expression of eye diseases should be studied as early as postnatal weeks 1-2 to ensure that any changes in gene expression pattern during disease development are detected. (molvis.org)

CRISPR-Cas9 editing technology-by virtue of its speed and unprecedented precision-has been a boon for scientists trying to understand the role of genes in disease. (biosafety-info.net)

Instead, the drugs squelch the dangerous side of a brain chemical called glutamate by turning on at least one gene, thereby increasing the number of "highways," or transporters, that remove glutamate from nerves. (scienceblog.com)
Rats and mice that received daily ceftriaxone for up to a week had triple the usual amount of the transporter, known as GLT1, in their brain cells, an effect that lasted for up to three months after treatment. (scienceblog.com)
Differential promoter methylation and histone modification contribute to the brain specific expression of the mouse Mbu-1 gene. (nih.gov)
Characterization of a novel mouse brain gene (mbu-1) identified by digital differential display. (nih.gov)
Taip2 is a novel cell death-related gene expressed in the brain during development. (nih.gov)
Here, we used a gene therapy approach to directly overexpress APPsα in the brain using AAV-mediated gene transfer and explored its potential to rescue structural, electrophysiological and behavioral deficits in APP/PS1∆E9 AD model mice. (springer.com)
Raw RNA locations of a sagittal mouse brain section produced by EEL FISH for 440 genes. (figshare.com)
Similar to the hormone insulin, which is responsible for making and controlling glucose levels in our circulation, the gene promotes fetal growth and plays a key part in the development of fetal tissues including the placenta, liver and brain. (technologynetworks.com)

The progression of emphysema in mice exposed to elastase was significantly improved by the gene therapy as evidenced by improvements in lung compliance and alveolar size," said Andrew Wilson, MD, lead author of the study and an assistant professor of medicine at BUSM. (sciencedaily.com)
6 ], the transforming growth factor beta 1 ( Tgfβ1 ) gene and several collagen genes were significantly downregulated in 8-week-old B6 mice scleras when compared with 3-week-old B6 mice scleras. (molvis.org)
In addition, cardiac lactate levels were significantly correlated to both PGC-1α and COX-IV cardiac gene expression . (bvsalud.org)

The genetic study showed promising results in "silencing" the genes that translate into tau protein, the primary component behind dementia. (medicaldaily.com)
Genome editing was previously used to restore vision in mice with the genetic eye disease Leber congenital amaurosis, which affects the retinal pigment epithelium, a layer of non-neuronal cells in the eye that supports the light-sensing rod and cone photoreceptor cells. (scienceboard.net)
National Eye Institute scientists have mapped the genetic architecture that determines gene expression and disease phenotype in human age-related eye. (scienceboard.net)
Mice born with a genetic defect that renders them infertile have given birth to litters of pups thanks to a gene-editing technique. (iflscience.com)
Genetic basis for susceptibility to noise -induced hearing loss in mice. (cdc.gov)
These genetic effects implicate the Ahl gene as contributing to NIHL susceptibility. (cdc.gov)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

All nucleotide sequences are aligned to the mm10 mouse reference, and amino acids are translated according to the reference frame (note that this implies that the frameshifts in the individual sequences are not shown). (elifesciences.org)

The recent identification of mammalian circadian clock genes now makes it possible to examine time zone adjustments from the perspective of molecular events within the suprachiasmatic nucleus (SCN), the principal circadian oscillator. (jneurosci.org)

though various " exercise pills " have successfully mimicked the effects of regular cardiovascular activity in mice, scientists still don't fully understand how, at a molecular level, exercise has the effects it does on the human body. (singularityhub.com)
An international group of scientists working with the European Mouse Disease Clinic (EUMODIC) altered 320 genes within individual mouse lines and put the animals through a battery of tests to understand what each gene did. (the-scientist.com)
Scientists gave cancerous lab mice a gene therapy that turned off the production of the Myc protein, which causes tumor growth. (medicaldaily.com)
In pregnant mice, scientists selectively altered the signalling cells in the placenta that tell mothers to allocate nutrients to her developing fetuses. (technologynetworks.com)

To investigate the possible effects of the expression of this tightly regulated gene in vivo, we produced transgenic mice in which CD40L expression was deregulated. (nki.nl)

We establish and characterize the Bckdha ( branched chain keto acid dehydrogenase a ) −/− mouse that exhibits a lethal neonatal phenotype mimicking human MSUD. (nature.com)
BCKDHA gene transfer rescued the lethal phenotype. (nature.com)

Image showing the signalling cells (in green) of the mouse placenta that are key for remote controlling the metabolism of the mother to support nutrient supply and growth of the fetus. (technologynetworks.com)
Although pregnancy is largely cooperative, there is a big arena for potential conflict between the mother and the baby, with imprinted genes and the placenta thought to play key roles. (technologynetworks.com)

Since multiple transcript and protein variants expressed by the same gene are, by definition, structurally distinct and need not to be functionally equivalent, the concept of gene orthology should be extended to the transcript level in order to describe evolutionary relationships between structurally similar transcript variants. (biomedcentral.com)
In other words, the identification of true orthology relationships between gene products now should progress beyond primary sequence and "splicing orthology", consisting in ancestrally shared exon-intron structures, is required to define orthologous isoforms at transcript level. (biomedcentral.com)
The observation that alternative splicing produces large numbers of alternative transcripts and proteins, some of them conserved across species and others truly species-specific, suggests that, still maintaining the conventional definition of gene orthology, a new concept of "splicing orthology" can be defined at transcript level. (biomedcentral.com)
Strict orthology of genes requires vertical descent from a common ancestor in the absence of intervening gene duplications, and it is often interpreted as indicating functional equivalence. (biomedcentral.com)

We applied this novel approach to achieve sustained in vivo expression of normal human alpha-1 antitrypsin (hAAT) protein at levels able to ameliorate emphysema in mice," said senior author Darrell Kotton, MD, an associate professor of medicine and pathology and co-director, Center for Regenerative Medicine at BUSM. (sciencedaily.com)
Our results challenge the dogma that lung macrophages are short-lived and suggest these differentiated cells as a target cell that may be considered for in vivo gene therapy applications including the sustained correction of hAAT deficiency," added Wilson. (sciencedaily.com)
The findings demonstrate cell rescue by in vivo gene transfer, thus supporting the feasibility of treating an inherited retinal degeneration by somatic gene therapy. (nih.gov)

According to the Foundation of Biomedical Research, 95% of animals used in scientific research are mice and rats, with 3.97 million experiments carried out on animals in the UK in 2017. (verdict.co.uk)

Sustained APPsα overexpression in aged mice with already preexisting pathology and amyloidosis restored synaptic plasticity and partially rescued spine density deficits. (springer.com)
Overexpression in mature T cells is compatible with life, but in one-third of the cases, mice developed atypical lymphoid proliferations which, occasionally, progressed into frank lymphomas. (nki.nl)

In a study done by a team at Washington University in St. Louis' medical school, mice quickly built muscle mass and reduced obesity after receiving the therapy, even while eating a diet high in fat and not exercising. (singularityhub.com)
Because we study ALS, we tested the drugs in a mouse model of that disease, but this is much bigger than ALS. (scienceblog.com)
This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150 mg/l NaF for 49 days. (greenmedinfo.com)
The fetal research study was patterned after an earlier successful trial of the gene editing process in anemic adult mice. (uconn.edu)
Bahal served as the lead author of the adult mice study, which was reported in Nature Communications in 2016. (uconn.edu)
In the present study, we combined masseter muscle tendon ligation (MMTL)-produced myogenic TMD with systemic injection of nitroglycerin (NTG)-induced migraine-like hypersensitivity in mice. (iasp-pain.org)
A conditional knockout resource for the genome-wide study of mouse gene function. (genscript.com)
The present study provides evidence that cardiac lactate levels are associated to gene transcription during an acute bout of high intensity running exercise . (bvsalud.org)
The present study demonstrates segregation for the putative Ahl gene and mapping of such a gene to Chromosome 10, consistent with other independent mapping of Ahl for AHL in 10 strains of mice [Johnson et al. (cdc.gov)

new nomenclature, gene organization, and divergence of human and rodent genes. (genscript.com)

The ARG1 gene is found on chromosome 6 . (medlineplus.gov)

If every condition, process, and trait in our bodies is tightly linked to our genes, it's conceivable that almost any aspect of our health could be manipulated using gene therapy and related tools. (singularityhub.com)
Even though gene therapy is one of the most promising approaches to cure human hyper IgM syndrome, these results suggest that when we modify very tightly regulated genes such as cytokines or other growth factors, particular care has to be taken to avoid excessive stimulation of the target cells. (nki.nl)

A gene therapy trial performed on mice may foreshadow yet another way to hack fitness. (singularityhub.com)
They also experienced reduced damage related to osteoarthritis, less inflammation in their joints, and had healthier hearts and blood vessels than mice that didn't receive the gene therapy -even though all the mice ate the same high-fat diet and did the same amount of exercise. (singularityhub.com)
If the muscle-building gene therapy eventually reaches a point where it can be used in humans, though, the research team isn't viewing it as a quick-fix health hack. (singularityhub.com)
Here we show efficacy of gene therapy for MSUD demonstrating its potential for clinical translation. (nature.com)
As an autosomal recessive monogenic disease, MSUD represents an ideal target for liver-directed gene therapy since clinical OLT data suggests that incomplete restoration of liver BCKD enzyme activity (representing 9-13% of body BCKD activity 10 ) is fully therapeutic. (nature.com)
Human Gene Therapy Methods 23:18-28. (springer.com)
For these reasons, the CD40L gene is a good candidate for gene therapy studies. (nki.nl)
Considered to be the most promising viral vectors for gene therapy, AAVs are able to deliver macromolecules into cells via a process called transcytosis. (iflscience.com)
Overall, they conclude that "our results, although in mice, suggest that some cases of female infertility in humans may be treated by AAV-mediated gene therapy. (iflscience.com)

Structural changes and neuropathology in the hypothalamus have been suggested to contribute to the non-motor manifestations of Huntington's disease (HD), a neurodegenerative disorder caused by an expanded cytosine-adenine-guanine (CAG) repeat in the huntingtin (HTT) gene. (lu.se)

Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from MSM/Ms mice. (nih.gov)
For example, of 38 lung modules there is a module associated with allergy, and seen only in the allergy model, containing over 100 genes and another module associated with T cells containing over 200 genes. (verdict.co.uk)
Their machine-learning algorithm can identify backup genes that only tumor cells are using so that drugs can target cancer precisely. (sflorg.com)
This approach is known as collateral lethality-using information gleaned from genes that cancer cells discard to find weaknesses. (sflorg.com)
Cancer cells themselves used to contain suppressor genes that prevent their spread. (sflorg.com)
In doing so, the cells typically lose other genes that are necessary for survival. (sflorg.com)
To avoid death, the cells find a paralog-a gene that can serve a similar function. (sflorg.com)
When a direct replacement for the deleted metabolic gene is not available, our algorithms use a mathematical model of the cancer cells' metabolism to predict the paralogous metabolic pathway they might use," said Abhinav Achreja, a U-M research fellow in biomedical engineering and lead author on the research paper. (sflorg.com)
In examining ovarian cancer cells, U-M's team zeroed in on one gene, UQCR11, that was often deleted along with a suppressor gene. (sflorg.com)
Top: Rows of healthy sensory hair cells in the mouse inner ear with green stereocilia arcs. (hearingreview.com)
Middle: In Rest mutant mice, hair cells are disorganized, and stereocilia barely visible. (hearingreview.com)
Bottom: In Rest mutant mice treated with HDAC inhibitors, organization and structure of hair cells is partially restored. (hearingreview.com)
When Banfi's team deleted exon 4 of Rest in mice, inner ear hair cells died, and mice became deaf. (hearingreview.com)
Many genes that should have been active were shut off in hair cells prior to their death. (hearingreview.com)
Cytosolic T3-binding protein modulates dynamic alteration of T3-mediated gene expression in cells. (harvard.edu)
Retinoic Acid and IFN Induce Parallel Arginase-1 and Nos2 Gene Expression in Primary Mouse Kupffer Cells. (gradientcorp.com)

This constitutes a strong rationale for testing liver gene transfer as a therapeutic option for MSUD. (nature.com)
GSK3 inhibition in liver reduces expression of the gluconeogenic genes PEPCK (phosphoenolpyruvate carboxykinase), G6Pase (glucose-6-phosphatase), as well as IGFBP1 (insulin-like growth factor binding protein-1). (portlandpress.com)

This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon. (genscript.com)
Alignment of the ORF of Gm13030 among the mouse populations, subspecies and related species where the sequence could be identified in the respective genomic region. (elifesciences.org)

Professor Amanda Sferruzzi-Perri, Professor in Fetal and Placental Physiology, a Fellow of St John's College and co-senior author of the paper, said: "It's the first direct evidence that a gene inherited from the father is signalling to the mother to divert nutrients to the fetus. (technologynetworks.com)
The baby's genes controlled by the father tend to promote fetal growth and those controlled by the mother tend to limit fetal growth. (technologynetworks.com)
Professor Sferruzzi-Perri explained: "Those genes from the mother that limit fetal growth are thought to be a mother's way of ensuring her survival, so she doesn't have a baby that takes all the nutrients and is too big and challenging to birth. (technologynetworks.com)

These modules represent clusters of genes that are co-regulated and can often be annotated to determine their function and known physiological roles. (verdict.co.uk)
D ) Comparison of fractions of sequence covered by hydrophobic clusters between de novo and all other protein-coding genes. (elifesciences.org)

Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. (greenmedinfo.com)

Specifically, genes associated with energy metabolism, cell proliferation, muscle myosin isoforms, as well as immune functions were found to be altered. (figshare.com)
A typical human gene, then, produces multiple transcript isoforms (ten per gene, on average), that can differ both in their coding and untranslated regions. (biomedcentral.com)

The lung macrophages carrying the therapeutic gene survived in the lungs air sacks for the two-year lifetime of the treated mice following a single intra-tracheal injection of the lentiviral vector we had engineered," he added. (sciencedaily.com)
T2Ps isolated from CNT-exposed lungs were found to have upregulated proinflammatory genes, including interleukin 1β (IL-1β), in contrast to those from CA exposed. (cdc.gov)

Lactate modulates cardiac gene expression in mice during acute physical exercise. (bvsalud.org)

Over the next decade, EUMODIC said it plans to identify roles for 20,000 mouse genes that will be stored in a database of mouse gene functions by the International Mouse Phenotyping Consortium (IMPC). (the-scientist.com)
Ratigan then sets out for Buckingham Palace, where his henchmen hijack the royal guards' roles and kidnap the Mouse Queen. (wikipedia.org)

Therefore one needs to follow up whole genome sequencing with other molecular profiling analyses or "omics": transcriptomics - gene expression profiling, proteomics - protein composition profiling, metabolomics - profiling of metabolites, and miR-omics - microRNA profiling. (biosafety-info.net)
Functional and evolutionary analyses of genes are usually performed on one or few representatives of their expression products, i.e. transcripts and proteins. (biomedcentral.com)

Finally, we integrated the expression data with scans for selection in natural populations and found substantial parallelism in the two northern populations for genes under selection. (biorxiv.org)

Here, we compared evolved gene expression differences between a warm-adapted house mouse strain and two different cold-adapted strains that have independently evolved similar phenotypic traits, such as increased body size and decreased extremity length during the last few hundred years. (biorxiv.org)

Together, our work describes the gene regulatory dynamics of rapid environmental adaptation, and the repeatability of these patterns over multiple instances of adaptation. (biorxiv.org)
second, to provide a more detailed annotation and quantification of true human-mouse orthologous transcripts defined as transcripts of orthologous genes exhibiting the same splicing patterns. (biomedcentral.com)

French biotechnology firm GenSight Biologics is highlighting its lenadogene neparvovec (Lumevoq) and GS030 gene therapies at the Cell & Gene Meeting. (scienceboard.net)
The technique also has raised hope for more powerful gene therapies that can delete or repair flawed genes, not just add new genes. (biosafety-info.net)

Carbon nanotube and asbestos exposures induce overlapping but distinct profiles of lung pathology in non-swiss albino CF-1 mice. (cdc.gov)

Biologists at the university introduced a specific gene responsible for enhanced cellular repair and protection into mice, which they say may be a step toward unlocking the secrets of aging and potentially extending human lifespan. (wgnradio.com)

Based on these findings, it was proposed that oxidative stress induced by excessive ROS may trigger sperm apoptosis through mitochondrial impairment, resulting in decreased fertility in mice exposed to fluoride. (greenmedinfo.com)
We used population level selection scans in wild animals to identify regions of the genome under selection and combined these findings with the gene expression data to identify candidate genes underlying adaptation to novel environments. (biorxiv.org)

In practice, two genes α and β are usually annotated as orthologous if their products are the "best reciprocal hits": that is, one of the proteins encoded by the α gene of species A has, as its most similar counterpart in species B, one of the proteins encoded by the β gene, and vice versa. (biomedcentral.com)

Mutations18

Differentially expressed genes1

RefSeq2

Inbred Strains1

Researchers25

Phenotypes2

Tissue8

Wild-type mice3

Mutation7

Regulation6

20231

Musculus1

Pathways4

MRNA4

Adeno-assoc2

CDNA3

Enrichment Analysis2

Diseases4

CRISPR1

Brain8

Significantly3

Genetic7

Sequences1

Mammalian1

Scientists4

Transgenic1

Phenotype2

Placenta2

Orthology4

Vivo3

Rats1

Overexpression2

Study9

Divergence1

Chromosome1

Tightly2

Therapy9

Hypothalamus1

Cells16

Liver2

Genomic2

Fetal3

Clusters2

Microarray1

Isoforms2

Lungs2

Modulates1

Roles2

Analyses2

Populations1

Strain1

Patterns2

Therapies2

Pathology1

Cellular1

Findings2

Counterpart1