• Our analysis identified five genes (GTF2H2, KLHL5, LRRC37A4P, PRR24, and MRPL23) that were significantly differentially expressed in the tissue of patients with neuropathic pain compared to controls. (iasp-pain.org)
  • XLS) pone.0182983.s008.xls (35K) GUID:?5238B94B-24E2-4BD0-B745-1CB33B5FB5D0 S2 Table: Significantly differentially expressed genes with FC5. (woofahs.com)
  • The results provide evidence that genes involved in tissue remodeling and repair are differentially regulated in the lungs of obstructed smokers and suggest that they are potential therapeutic targets. (nih.gov)
  • RNA sequencing of the gills identified genes that were differentially expressed in association with the salinity change and associated with the loss of saltwater tolerance in the Toltén population . (bvsalud.org)
  • As the suite of differentially expressed genes in the diadromous-resident comparison differed between freshwater populations , we hypothesize that diadromy loss results in unique evolutionary trajectories due to drift, so the loss of diadromy does not necessarily lead to a loss in upper salinity tolerance . (bvsalud.org)
  • Expression data were measured using microarrays, and analyzed to identify differentially expressed genes and elucidate underlying biology. (immuneering.com)
  • Differentially expressed genes induced by proposed generic vs. GA were associated with activation of inflammatory responses and increased cell adhesion and migration. (immuneering.com)
  • Proposed generic upregulated inflammatory monocyte-related genes to a greater extent than GA. Additional genes differentially expressed between GA vs. proposed generic, and amongst proposed generics from different manufacturers, also may play a role in MS pathology and therapeutic response. (immuneering.com)
  • It has become a prevalent technology, allowing an efficient genome-wide relative quantification of gene expression and, in particular, it is the method of choice to find differentially expressed genes between two or more biological conditions of interest. (frontiersin.org)
  • In this study, we performed a new bioinformatics analysis on microarray-based gene expression data obtained from the public domain to explore genes that were differentially induced by drugs between the group of capreomycin and PA-824 and the group of mainly the first-line TB drugs. (hindawi.com)
  • We identified differentially expressed genes from 14 case-control human breast cancer gene expression datasets and integrated them with drug-protein networks. (sutterhealth.org)
  • Cells regulate the expression of their genes with proteins called transcription factors,' said co-corresponding author Dr. Josephine C. Ferreon, assistant professor of pharmacology and chemical biology and member of the Dan L Duncan Comprehensive Cancer Center at Baylor. (news-medical.net)
  • A cell culture system is here proposed opening new integrative studies on biomineralization, improving our knowledge on the role played by non-collagenous proteins in bone. (sorbonne-universite.fr)
  • A temporal correlation was established between quantified gene expression of DMP1 and IBSP, and the presence of hydroxyapatite, confirming the contribution of these proteins to the mineralization process. (sorbonne-universite.fr)
  • Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. (wikipedia.org)
  • These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA. (wikipedia.org)
  • Gene expression in living cells is strictly self-regulated to ensure that the correct amounts of proteins are made at the most appropriate timing and location for maintaining cellular homeostasis. (nature.com)
  • Although non-coding RNAs fail to produce functional proteins, researchers believe that in some cases these RNAs may control gene expression. (sciencedaily.com)
  • Genes encoding disrupted complementary interactors tend to be risk genes, and an interaction network built from these proteins is enriched for ASD proteins. (springer.com)
  • Real-time quantitative PCR is a powerful technique for the investigation of comparative gene expression, but its accuracy and reliability depend on the reference genes used as internal standards. (edu.au)
  • Only genes that show a high level of expression stability are suitable for use as reference genes, and these must be identified on a case-by-case basis. (edu.au)
  • The identification of stable internal reference genes for this species is important for its development as a model species, and would enable comparative analysis of candidate biosynthetic genes in the different tissues of the coca plant. (edu.au)
  • From our results we conclude that Ec10131 and TPB1 are the most appropriate internal reference genes in leaves (where the majority of cocaine is produced), while Ec10131 and Ec6409 are the most suitable internal reference genes across all of the tissues tested. (edu.au)
  • Regulation of gene expression gives control over the timing, location, and amount of a given gene product (protein or ncRNA) present in a cell and can have a profound effect on the cellular structure and function. (wikipedia.org)
  • Regulation of gene expression is the basis for cellular differentiation, development, morphogenesis and the versatility and adaptability of any organism. (wikipedia.org)
  • Gene regulation may therefore serve as a substrate for evolutionary change. (wikipedia.org)
  • Gene regulation can occur at any point in gene expression, from the start of the transcription phase to the translation phase. (nature.com)
  • Due to the complexity of gene regulation, unveiling the complete mechanism of gene regulation has been a long-standing quest. (nature.com)
  • To elucidate the complex mechanism of gene regulation in living cellular systems, it is necessary to closely investigate each phase of gene expression and also to shed light on the cooperative effects between transcription and translation. (nature.com)
  • The aforementioned complex factors have made it difficult to investigate the impact of the DNA length on gene regulation, which has been an unexplored topic. (nature.com)
  • Instead, the non-coding RNA was suspected to play a role in hGH gene regulation. (sciencedaily.com)
  • Although glucocorticoid receptors (GRs) in the hippocampus play a vital role in the regulation of physiological and behavioural responses to stress, the regulation of receptor expression remains unclear. (karger.com)
  • studies on cell cycle regulation and genome instability. (ncl.ac.uk)
  • The recent identification of mammalian circadian clock genes now makes it possible to examine time zone adjustments from the perspective of molecular events within the suprachiasmatic nucleus (SCN), the principal circadian oscillator. (jneurosci.org)
  • Get inducible gene expression for mammalian systems with SBI. (systembio.com)
  • Early application of second-generation sequencing technologies to transcript quantitation (RNA-seq) has hinted at a vast mammalian transcriptome, including transcripts from nearly all known genes, which might be fully measured only by ultradeep sequencing. (biomedcentral.com)
  • 8 ] demonstrated that gene expression in mammalian cells measured by RNA-seq follows a bimodal distribution of high and low expression genes, and suggested that the high-expression genes comprise the active, functional transcriptome of the cell. (biomedcentral.com)
  • The early findings suggest space travel boosts methylation, the process of switching genes on and off. (upi.com)
  • Formation of this biomolecular condensate is strongly enhanced by a DNA modification called CpG methylation, a change that influences gene expression,' said co-corresponding author Dr. Kevin MacKenzie, associate professor of pathology and immunology and of pharmacology and chemical biology at Baylor. (news-medical.net)
  • This was related to an increased DNMT3a protein association with a discreet region of the Nr3c1 (GR gene) promoter, shown here to undergo increased DNA methylation after FSS. (karger.com)
  • These developments will allow us to study in parallel the transcriptional program and components of the epigenetic program (nuclear position, timing of replication, chromatin structure and modification, DNA methylation) to determine the hierarchy and order of events required to switch genes on and off during differentiation and development. (ox.ac.uk)
  • 2017. An epigenome-wide DNA methylation study of PTSD and depression in World Trade Center responders . (cdc.gov)
  • A similar study in rats revealed that approximately the same sample size was required for rat brain mRNA expression studies.The mRNA levels of several genes were studied in 55 schizophrenia and 55 control prefrontal brain autopsies, using a novel and more powerful statistical analysis. (avhandlingar.se)
  • The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)
  • XLS) pone.0182983.s014.xls (62K) GUID:?04D9F0F7-81E4-4D80-A3AD-12575347D4BE Data Availability StatementAll microarray data supporting the conclusions of this article are available in the Gene Expression Omnibus database with the accession number GSE81058 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE81058). (woofahs.com)
  • CONCLUSIONS: Important levels of expression of GPD1 (but not of other stress genes) are required during the first hours of vinification, because of the need for glycerol production to counteract the hyperosmotic stress at this point. (unboundmedicine.com)
  • CONCLUSIONS: This is a proof-of-concept study demonstrating that a combination of complementary data sources, such as EHRs and gene expression, can corroborate discoveries and provide mechanistic insight into drug synergism for repurposing. (sutterhealth.org)
  • We provide an updated view of the accuracy and efficiency of RNA-seq experiments, using genomic data from large-scale studies like the ENCODE project to provide orthogonal information against which to validate our conclusions. (biomedcentral.com)
  • This study aimed to identify genes associated with neuropathic pain through transcriptome analysis in order to gain a better understanding of the mechanisms underlying this chronic, difficult-to-treat pain. (iasp-pain.org)
  • Gene ontology (GO) term enrichment analysis was used to highlight the most relevant GO terms associated with a given gene list following toxin exposure. (plos.org)
  • Gene ontology: tool for the unification of biology. (microbiologyresearch.org)
  • DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
  • We also analyzed the impact of both the replicate number and library size on gene ontology (GO) enrichment analysis. (frontiersin.org)
  • A team led by researchers at Baylor College of Medicine has discovered a mechanism by which transcription factor KLF4 can help to organize chromatin, thus influencing gene expression. (news-medical.net)
  • Transcription factors such as KLF4 are involved in reorganizing the chromatin to provide access and facilitate gene transcription, but it is not clear how this is accomplished. (news-medical.net)
  • When KLF4 interacts with specific chromatin regions, it forms a condensate - a separate liquid phase - that preferentially recruits other molecules that help open the chromatin and mediate gene transcription. (news-medical.net)
  • Gene expression" refers to the process by which a DNA sequence (i.e. a gene) is used to make a specific RNA or protein, while "epigenetics" refers to the ways phenotypes are impacted by factors other than genetic sequence (e.g. gene repression by chromatin packing). (umassmed.edu)
  • Using genomics to study how chromatin influences gene expression. (ox.ac.uk)
  • The Rando Lab studies the role of epigenetic inheritance in programming health and disease. (umassmed.edu)
  • Are there epigenetic changes in specific immune genes of patients with PTSD that may predispose them to respiratory illness? (cdc.gov)
  • In Expression Atlas, users can query genes or gene-sets of interest and explore their expression across or within species, tissues, developmental stages in a constitutive or differential context, representing the effects of diseases, conditions or experimental interventions. (oregonstate.edu)
  • Novel features and visualizations include the on-the-fly analysis of gene set overlaps and the option to view gene co-expression in experiments investigating constitutive gene expression across tissues or other conditions. (oregonstate.edu)
  • The expression of prostanoid receptor genes in uterine and fetal tissues. (gla.ac.uk)
  • 1. The aim of this project was to determine whether advancing gestational age and parturition were associated with alteration in the relative level of expression of genes encoding prostanoid receptors in key uterine and fetal tissues. (gla.ac.uk)
  • I also sought to determine whether advancing gestational age and parturition were associated with alteration in the expression of genes encoding lipoxygenase (LOX) enzymes in key intra-uterine tissues. (gla.ac.uk)
  • Central to this investigation is an understanding of what gene products are required and expressed during a natural infection and how this expression changes over time (from initial colonization to causation of disease and spread of the pathogen to new hosts) and space (in different cells or tissues within the host). (cdc.gov)
  • Scientists have mapped which genes are turned on and off in the different tissues and organs in a sheep's body. (ed.ac.uk)
  • RNA serves as a functional read-out of exactly which genes are expressed in which tissues at any one time. (ed.ac.uk)
  • The expression of these genes was measured in seven tissues (flowers, stems, roots and four developmental leaf stages) and the stability of expression was assessed using three algorithms (geNorm, NormFinder and BestKeeper). (edu.au)
  • 3 Initial studies of animals not in labour using cDNA probes demonstrated transcripts of similar size to the human genes for prostanoid EP2, EP3, EP4, and FP receptor mRNA using Northern blot in myometrium. (gla.ac.uk)
  • 4 When expression of prostanoid receptor genes was studied in myometrium obtained from animals both in labour and not in labour and the techniques employed were optimized (principally the use of riboprobes), transcripts of similar size to the human genes were detected for prostanoid EP1, EP2, EP3, EP4, IP, FP and TP receptor mRNA using Northern blot. (gla.ac.uk)
  • Gene expression was related to forced expiratory flow between 25 and 75% of forced expiratory volume (FEF(25-75%) % predicted) revealing a signature gene set of 203 transcripts. (nih.gov)
  • moreover, most RNA-seq experiments did not provide enough read depth to generate high-confidence estimates of gene expression for low-abundance transcripts. (biomedcentral.com)
  • Increased read length improves mapping accuracy and lowers the odds of spurious multiple mapping, while greater read depth allows more accurate assessment of the relative abundance of low-expression transcripts as well as the detection (by at least one read mapping) of a greater number of genes [ 5 ]. (biomedcentral.com)
  • Of patients with FSHD, 50-75% exhibit retinal vasculopathy and increased expression of vascular or endothelial-related FRG1 transcripts in the muscle. (medscape.com)
  • Methods of studying differential GENE EXPRESSION of especially low-abundance, tissue-specific gene transcripts. (bvsalud.org)
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
  • The serotonin receptor 2C gene (HTR2C) showed a significant 1.5-fold decrease in the patients as compared to controls, and the monoamine oxidase B gene (MAOB) a 1.2-fold increase.The mechanism behind the decrease of HTR2C mRNA levels was investigated by studying the correlation of drug treatment and HTR2C promoter polymorphisms to the HTR2C expression levels. (avhandlingar.se)
  • For all these genes, mRNA levels increase as temperature decreases or pH increases. (unboundmedicine.com)
  • FSS also caused a time-dependent increase in the expression of miR-124a, a microRNA known to reduce GR mRNA expression, which was inversely correlated with a reduction in GR mRNA levels 30 min after FSS. (karger.com)
  • Interestingly, both TGF-beta1 and SERPINE2 have been identified as candidate genes in COPD genetic linkage and association studies. (nih.gov)
  • Dr. Pryciak studies how protein kinases regulate cell signaling and the cell cycle. (umassmed.edu)
  • Because the environment encountered within a living host will be quite different from the external environment, pathogens must be able to regulate the necessary genes in coordination as they move from the environment to the host and from one host niche to another. (cdc.gov)
  • The Wnt genes encode secreted glycoprotein ligands that regulate a wide range of developmental processes, including axis elongation and segmentation. (biomedcentral.com)
  • Data from large-scale RNA sequencing studies including Blueprint, PCAWG, ENCODE, GTEx and HipSci can be visualized next to each other. (oregonstate.edu)
  • We analyse by means of quantitative PCR the expression of several stress induced genes in 24 efficient commercial wine yeast strains at the beginning of vinifications performed under standard conditions or with small variations in pH and temperature. (unboundmedicine.com)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • Gene expression profiling of tumors using DNA microarrays is a promising method for predicting prognosis and treatment response in cancer patients. (lu.se)
  • XLSX) pone.0182983.s011.xlsx (22K) GUID:?430A4094-4A03-426B-AD26-C3C99AA539C3 Snap23 S5 Table: Expression of genes in the IE and RPE that are involved in genetic retinal diseases originating in the RPE. (woofahs.com)
  • To understand the molecular-genetic basis of functional specialization and identify potential drug targets specific to each neuron subtype, we performed a genome wide assessment of both gene expression and splicing across EXC, PV, SST and VIP neurons from male and female mouse brains. (jneurosci.org)
  • An extensive literature documenting physiological and ecological diversity make Daphnia an ideal model to study the genetic architecture of phenotypic variation in natural populations and the evolution of genome structure/function relationships in aquatic environments. (biomedcentral.com)
  • Although the genetic lesion is well described in FSHD, the causal gene and the protein products are not known. (medscape.com)
  • We work on large scale studies on the genetic basis of common diseases with complex causes. (ncl.ac.uk)
  • Although these factors have been studied extensively, little is known about how the length of the DNA template influences gene expression. (nature.com)
  • Gene expression differences between patients and controls can be used to find susceptibility genes and drug targets for a disease. (avhandlingar.se)
  • Lastly, we determined whether synergistic drug pairs found in the EHRs were enriched among synergistic drug pairs from gene-expression data using a method similar to gene set enrichment analysis. (sutterhealth.org)
  • Gene expression signature of human neuropathic pain identified through transcriptome analysis. (iasp-pain.org)
  • Expression of genes was quantified by Northern analysis as a ratio of the signal for the gene of interest to each of three housekeeping genes (glyceraldehyde-3-phosphate dehydrogenase [GAPDH], beta-actin and cyclophilin). (gla.ac.uk)
  • 5 When expression of prostanoid receptor genes was studied in cervix obtained from animals both in labour and not in labour, clear signals which were similar in estimated size to the human genes were detected by Northern analysis for EP1, EP2, EP3, EP4, FP, IP and TP receptors. (gla.ac.uk)
  • The authors declare that the sponsors had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
  • To overcome this limitation and perform analysis at the cellular level, we aimed to design bicistronic vectors to misexpress, in the same cell, the gene-of-interest and the reporter gene. (biologists.com)
  • TY - JOUR T1 - Analysis of the expression of some stress induced genes in several commercial wine yeast strains at the beginning of vinification. (unboundmedicine.com)
  • The additional information generated by whole genome studies goes far beyond that derived by characterizing in isolation more genes and gene products, because analysis of the whole genome allows complete regulatory networks to be identified and characterized. (cdc.gov)
  • Two of these methods, signature-tagged mutagenesis and in vivo expression technology, do not directly measure gene expression and do not allow true genomic-scale analysis, but they have been devised to identify genes necessary for pathogens during real infections. (cdc.gov)
  • Whole-genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation. (ethz.ch)
  • In silico analysis of available array data showed that a large number of genes traditionally classified as PEP dependent genes are transcribed during early embryo development from the pre-globular stage to the mature-green-stage. (frontiersin.org)
  • Ten DEGs, including 7 hub genes from PPI analysis, were confirmed by real-time PCR. (medscimonit.com)
  • RNA-Seq is a widely used technology that allows an efficient genome-wide quantification of gene expressions for, for example, differential expression (DE) analysis. (frontiersin.org)
  • While the main drawback of previous relevant studies is the lack of generality, we conducted both an analysis of a two-condition experiment (with eight biological replicates per condition) to compare the results with previous benchmark studies, and a meta-analysis of 17 experiments with up to 18 biological conditions, eight biological replicates and 100 million (M) reads per sample. (frontiersin.org)
  • As a global trend, we concluded that the replicate number has a larger impact than the library size on the power of the DE analysis, except for low-expressed genes, for which both parameters seem to have the same impact. (frontiersin.org)
  • For individuals working on tomato research, on the basis of the meta-analysis, we recommend at least four biological replicates per condition and 20 M reads per sample to be almost sure of obtaining about 1000 DE genes if they exist. (frontiersin.org)
  • This resource, combining a unique new dataset and novel application of analysis methods to multiple relevant datasets, identifies numerous potential drug targets for manipulating circuit function, reveals neuron subtype-specific roles for disease-linked genes, and is useful for understanding gene expression changes observed in human patient brains. (jneurosci.org)
  • We used a genome-wide analysis which not only examined differential gene expression levels but could also detect differences in splice isoform usage. (jneurosci.org)
  • Ongoing comparative analysis will provide insights to help us understand gene function across all large animal species, including humans. (ed.ac.uk)
  • As a result, the community adopted several heuristics for RNA-seq analysis, most notably an arbitrary expression threshold of 0.3 - 1 FPKM for downstream analysis. (biomedcentral.com)
  • This includes the analysis of genes in germline stem cell proliferation and differentiation. (ncl.ac.uk)
  • ER- status has been shown to Recent studies have shown that microarray analysis of leave a remarkable imprint on the expression profile of a tumors generate molecular phenotypes that can be used tumor, and may have confounding effects on the clinical to classify tumors into subgroups that are not evident by factor studied [7-9]. (lu.se)
  • The first example describes a research project involving sequencing based gene expression analysis and the second example describes a microarray service facility use of BASE. (lu.se)
  • Genes in this pathway have been shown to be involved in the activation of transforming growth factor (TGF)-beta1 and matrix metalloproteinases and are subject to inhibition by SERPINE2. (nih.gov)
  • We have previously utilised an NT2.D1 neurosphere based model of neuronal differentiation to study the effects of a variety of compounds on neural development [7] . (plos.org)
  • After performing limb somite electroporation using these vectors, we observed a simultaneous cellular expression of Tomato (membrane) and GFP (nuclei) at the different stages of muscle differentiation. (biologists.com)
  • We believe that this set of tools can be used to efficiently misexpress genes at different time points of myogenic cell differentiation and analyse the consequences for muscle development. (biologists.com)
  • Expression Atlas ( http://www.ebi.ac.uk/gxa ) is an added value database that provides information about gene and protein expression in different species and contexts, such as tissue, developmental stage, disease or cell type. (oregonstate.edu)
  • The electroporation technique is widely used in developmental biology to deliver foreign DNA into cells and study gene function. (biologists.com)
  • Expression Atlas: gene and protein expression across multiple studies and organisms. (oregonstate.edu)
  • Among persisting questions on bone calcification, a major one is the link between protein expression and mineral deposition. (sorbonne-universite.fr)
  • Impact of 60-GHz millimeter waves on stress and pain-related protein expression in differentiating neuron-like cells. (ethz.ch)
  • Although using these technologies to analyze pathogens within a host is still in its infancy, initial studies indicate that these technologies will be valuable tools for understanding how the pathogen reacts to the in vivo microenvironment. (cdc.gov)
  • The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
  • In the current study, we focused on master transcription factor KLF4, which is known to selectively mediate gene expression and reprogramming that determines cell fate. (news-medical.net)
  • Gene expression is summarized in the central dogma of molecular biology first formulated by Francis Crick in 1958, further developed in his 1970 article, and expanded by the subsequent discoveries of reverse transcription and RNA replication. (wikipedia.org)
  • All steps in the gene expression process may be modulated (regulated), including the transcription, RNA splicing, translation, and post-translational modification of a protein. (wikipedia.org)
  • While transcription of prokaryotic protein-coding genes creates messenger RNA (mRNA) that is ready for translation into protein, transcription of eukaryotic genes leaves a primary transcript of RNA (pre-RNA), which first has to undergo a series of modifications to become a mature RNA. (wikipedia.org)
  • Cell-free gene expression systems have been valuable tools for understanding how transcription/translation can be regulated in living cells. (nature.com)
  • To test whether the transcription of the locus control region and CD79b played a significant role in hGH expression in transgenic mice, Ho then inserted a special piece of DNA into the locus control region. (sciencedaily.com)
  • Forkhead box (Fox) transcription factors evolved early in animal evolution and represent important components of conserved gene regulatory networks (GRNs) during animal development. (lu.se)
  • This study will examine the potential mechanisms underlying PTSD/respiratory co-morbidity that may facilitate the development of more effective, theory-driven interventions for these difficult to treat patients. (cdc.gov)
  • The overall goal of this study is to explicate the mechanisms linking PTSD and lower respiratory symptoms (LRS) in WTC responders through an epigenetics approach. (cdc.gov)
  • Expression of EP1, EP3 and IP receptor mRNA was significantly higher in myometrium from the fundus (compared with lower segment) whereas EP2 gene expression was significantly lower in the fundus. (gla.ac.uk)
  • non-etheless, we also discovered major specific distinctions: For instance, genes and molecular pathways, linked to the visual circuit and retinol biosynthesis are higher portrayed in the RPE AZD6244 supplier than in the IE significantly. (woofahs.com)
  • We found that some of these "saltwater acclimation " genes , including the cystic fibrosis transmembrane conductance regulator gene (CFTR), were not significantly upregulated in the saltwater intolerant resident population (Toltén), suggesting a potential mechanism for the loss of tolerance to higher salinities. (bvsalud.org)
  • Statins have been associated with a significantly lower risk of breast (8), colorectal (9), and lymph cancers (10-12) in several observational studies (10,13). (cdc.gov)
  • A study by Fogh et al of induction chemoradiotherapy followed by surgery, a strategy that is widely used in treating esophageal cancer, found that perioperative morbidity and mortality with this approach was not significantly different in patients aged 70 years or older compared with younger patients. (medscape.com)
  • Environmental conditions experienced by parents can influence gene expression patterns in their offspring. (umassmed.edu)
  • Now, researchers at UC San Francisco and Northwestern Medicine, in collaboration with 10 other medical centers, have found a highly accurate way to predict the best treatment for patients based on patterns of gene expression - which genes are turned on and off - in their tumors. (ucsf.edu)
  • Using samples from 1,856 meningioma patients at 12 medical centers in the U.S., Europe and Hong Kong, Raleigh's team came up with a set of 34 genes whose gene expression patterns had the potential to predict whether a tumor would return. (ucsf.edu)
  • For genes encoding the disrupted interactions, we evaluate their expression patterns across developing brains and within specific cell types, using both bulk and inferred cell-type-specific brain transcriptomes. (springer.com)
  • Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)
  • EHRs provide real-world treatment and outcome patterns, while complementary biomolecular data, including disease-specific gene expression and drug-protein interactions, provide mechanistic understanding. (sutterhealth.org)
  • Although analyses that give information on the expression of a few genes provide insight and have been responsible for a large proportion of the bacterial pathogenesis literature currently available, our ultimate goal is to understand expression changes across the whole genome. (cdc.gov)
  • Their findings shed new light on the animal's complex biology, including insight into the function of genes linked to immunity and meat quality. (ed.ac.uk)
  • There were no gestational age related changes in expression of these genes. (gla.ac.uk)
  • This study represents one of the most comprehensive views of human biology," said Mason, an associate professor at Weill Cornell Medicine. (upi.com)
  • GA also upregulated FOXP3 and other genes associated with tolerance-inducing regulatory T cells (Tregs) more consistently and to a greater extent than proposed generic. (immuneering.com)
  • We show that although point mutations and structural variants that drive cancer are less frequent in non-coding genes and regulatory sequences than in protein-coding genes, additional examples of these drivers will be found as more cancer genomes become available. (lu.se)
  • The zFPKM normalization method accurately separates the biologically relevant genes in a cell, which are associated with active promoters, from the ultralow-expression noisy genes that have repressed promoters. (biomedcentral.com)
  • This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
  • Electroporation of limb somites allows the misexpression of genes in limb somite derivatives, like myogenic and endothelial cells, while electroporation of the lateral plate targets other limb cells like cartilage, bone and tendons. (biologists.com)
  • The p57MRE/βactin promoter, which drives expression in differentiated myoblasts, targeted mononucleated (myosin-) cells and muscle fibres (myosin+). (biologists.com)
  • Lateral plate electroporation with the vector containing the CMV/βactin promoter allowed Tomato and GFP co-expression in cartilage, tendons and connective tissue of the limbs, but never in myogenic (Pax7+ or myosin+) cells. (biologists.com)
  • Therefore, many studies in the last decade focused on the development and use of induced pluripotent stem cells (iPSC) as a source for autologous cell replacement therapy. (woofahs.com)
  • Immunohistochemistry confirmed expression of urokinase plasminogen activator (PLAU), urokinase plasminogen activator receptor (PLAUR), and thrombospondin (THBS1) by alveolar macrophages and airway epithelial cells. (nih.gov)
  • On long term cultures human bone cells seeded on the osteoid-like matrix displayed a clear osteoblast phenotype as revealed by the osteoblast-like morphology, expression of specific protein such as alkaline phosphatase and expression of eight genes classically considered as osteoblast markers, including BGLAP, COL1A1, and BMP2. (sorbonne-universite.fr)
  • 2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells. (ethz.ch)
  • Researchers at UC San Francisco and Johns Hopkins may have found a new way to diagnose Lyme disease, based on a distinctive gene "signature" they discovered in white blood cells of patients infected with the tick-borne bacteria. (universityofcalifornia.edu)
  • In an effort to find better ways of diagnosing the disease, and discovering molecular pathways that might explain how Lyme disease could cause long-term symptoms, researchers used a next-generation sequencing technique, called RNA-seq, to investigate the transcriptome - the genes that are being turned on - in peripheral blood mononuclear cells. (universityofcalifornia.edu)
  • The aim of this study was to investigate the expression and silencing of the S100A8 gene, which encodes the S100 calcium-binding protein A8 (S100A8), and apoptosis and phosphorylation of protein kinase B (Akt) in tissue samples of endometrial carcinoma and HEC-1A endometrial adenocarcinoma cells in vitro . (medscimonit.com)
  • A stable S100A8 gene knockdown cell line was constructed using lentiviral packing short hairpin RNA (shRNA) transfected into HEC-1A cells. (medscimonit.com)
  • The effects of expression of the S100A8 gene by endometrial cancer cells was investigated by the MTT assay, cell cycle and apoptotic assays, qRT-PCR, and Western blotting. (medscimonit.com)
  • Across cell types, these genes affect neuronal morphogenesis and neuronal communication, while neural progenitor cells show strong enrichment for development of the limbic system. (springer.com)
  • In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (who.int)
  • As of August 2017, Expression Atlas holds data from 3,126 studies across 33 different species, including 731 from plants. (oregonstate.edu)
  • In addition to the web interface, data sets can now be searched and downloaded through the Expression Atlas R package. (oregonstate.edu)
  • In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
  • We looked into the gene appearance information on both gene and useful network level, using R and the data database Ingenuity. (woofahs.com)
  • Data are available at the Gene Expression Omnibus under accession number GSE156478 . (nature.com)
  • Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
  • The first 2 pairs were also enriched among pairs discovered using gene expression data and are supported by molecular interactions in drug-protein networks and preclinical and epidemiologic evidence. (sutterhealth.org)
  • However, the data used in these studies were from short read RNA-seq (often 32-base single-end reads) of moderate depth (typically ~20 million reads). (biomedcentral.com)
  • These advances undermine the assumptions upon which previous heuristics for evaluating gene expression were based, highlighting the need for updated understanding of the signal and noise present in RNA-seq data. (biomedcentral.com)
  • This retrospective study was conducted by using the computerized data sets of a large health maintenance organization (HMO) in Israel. (cdc.gov)
  • Having observed an overlap in those data between the genes that predict outcome and those that predict estrogen receptor- status, we examined their predictive power in an independent data set. (lu.se)
  • ER- status is by far the most obvious and noticeable cal variables from gene expression data is complicated by subdivision of breast tumors based on gene expression a common correlation between ER- status and the clini- profiles. (lu.se)
  • 8]. genes that discriminate ER- status in their data and those that were reported by us and by West et al. (lu.se)
  • Analyses include generation of gene expression and sequencing data with the ultimate goal to build an infrastructure for future real-time clinical implementation. (lu.se)
  • This particular BASE installation was initially set up in 2003 and to date manage array data from more than 13 000 hybridisation covering a variety of technical platforms such as cDNA, oligo, and BeadChip expression arrays, as well as BAC and oligo aCGH arrays. (lu.se)
  • Misexpression of FRG1 (FSHD region gene 1) may lead to the development of FSHD. (medscape.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • Sin embargo, algunos genes involucrados en la aclimatación al agua salada , incluyendo el gen regulador de la conductancia transmembrana de la fibrosis quística (CFTR), no se diferenciaron significativamente en la población residente intolerante al agua salada (Toltén), sugiriendo un mecanismo potencial de la pérdida de tolerancia a ambientes con salinidad elevada. (bvsalud.org)
  • These findings suggest that these five genes may be potential therapeutic targets for neuropathic pain. (iasp-pain.org)
  • Six among the 42 genes identified in this study are on the list of the top 100 persistence targets selected by the TB Structural Genomics Consortium. (hindawi.com)
  • Gene expression provides a means to investigate the biological impact of these differences, particularly given the absence of PK/PD markers for GA. This represents a test case for the broader challenge of assessing biosimilars/biogenerics and generics for non-biological-complex-drugs. (immuneering.com)
  • We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)
  • The objective of this study was to investigate the association between persistent statin use and the risk of primary cancer in adults. (cdc.gov)
  • The objective of this study was to investigate the association between persistent use of statins and the risk of overall and site-specific cancer in adults, to assess dose-response relationship, and to examine the effects of varying types of statins. (cdc.gov)
  • OBJECTIVES: The aim of this study was to investigate the genotoxicity of waterpipe smoking in the lymphocytes of waterpipe smokers using chromosomal aberrations (CAs) assay. (who.int)
  • Most of the researches concerning Fox genes, however, are on vertebrates and only a relatively low number of studies investigate Fox gene function in invertebrates. (lu.se)
  • Treatment with VPA and LiCl resulted in the differential expression of 331 and 164 genes respectively. (plos.org)
  • The study, published in the journal Nature Communications, reveals that the binding of KLF4 can cause DNA to condense into a separate liquid phase in a process called biomolecular condensation, which recruits other factors that influence gene expression. (news-medical.net)
  • Oct. 25 (UPI) -- NASA has announced some preliminary results from its Twins Study, an effort to study the effects of space travel on human health. (upi.com)
  • Scientists expect to publish finalized results from the Twins Study in 2018. (upi.com)
  • These results cannot be achieved with a "one-gene-at-a-time" approach. (cdc.gov)
  • Acute stress results in decreased GR mRNA expression specifically in the dentate gyrus. (karger.com)
  • These results reveal the potential for dissociation of mPer and mCry expression within the central oscillator during circadian resetting and a differential molecular response of the clock during advance and delay resetting. (jneurosci.org)
  • In a paper appearing Nov. 9, 2023 in Nature Medicine , the team concluded that just 1 in 5 patients with low-grade tumors (those less likely to regrow) may need radiation, while around 2 in 5 with higher-grade tumors may be better off without radiation, based on the results of the new gene-expression test. (ucsf.edu)
  • These results reveal numerous examples where neuron subtype-specific gene expression, as well as splice-isoform usage, can explain functional differences between neuron subtypes, including in presynaptic plasticity, postsynaptic receptor function, and synaptic connectivity specification. (jneurosci.org)
  • The results of several studies constrain the range of the threshold that divides active from low-expression genes: at the upper bound, Hebenstreit et al . (biomedcentral.com)
  • Deletion of D4Z4 macrosatellites results in aberrant gene expression. (medscape.com)
  • The level of synaptophysin messenger RNA paralleled that of synaptophysin immunoreactivity, indicating that changes in gene expression contribute to variations in the latter observed in aging and other situations. (ox.ac.uk)
  • To our knowledge, this study is the first to document changes in gene expression occurring even after a bacterial infection has been treated with appropriate antibiotics," said John Aucott, MD, assistant professor of medicine at the Johns Hopkins University School of Medicine and a senior investigator on the study. (universityofcalifornia.edu)
  • Researchers recently discovered a novel mechanism that works over an extensive genomic distance and controls the expression of human growth hormone (hGH) in the pituitary gland. (sciencedaily.com)
  • Researchers at the University of Pennsylvania School of Medicine recently discovered a novel mechanism that works over an extensive genomic distance and controls the expression of human growth hormone (hGH) in the pituitary gland. (sciencedaily.com)
  • While mutations to the hGH gene often lead to abnormal growth in children and adults, these mutations have provided researchers with key clues regarding the genomic areas that appear to control expression of the hGH gene. (sciencedaily.com)
  • At the genomic level, a 14,000 base-pair separation is equal to the size of 10 growth hormone genes lined end to end. (sciencedaily.com)
  • Detection of synaptophysin messenger RNA broadens the range of methods by which synaptic protein gene products can be studied and used as markers of synaptic density and synaptic involvement during physiological and pathological processes in human brain. (ox.ac.uk)
  • Some of the most exciting things that we've seen from looking at gene expression in space is that we really see an explosion, like fireworks taking off, as soon as the human body gets into space," Chris Mason , lead researcher on the Twins Study, said in a news release late Tuesday. (upi.com)
  • Indeed, both the hESc and human neuroprogenitor cell (hNPC) models provide valid and useful tools for studying DNT. (plos.org)
  • Comparison of Hsps expression after radio-frequency field exposure in three human glioma cell lines. (ethz.ch)
  • The human genome is comprised of both non-coding DNA and coding regions, or genes. (sciencedaily.com)
  • The effects of the locus control region on human growth hormone expression is as if you turn a key in the lock of a house at one end of your street and find that this action opens the lock and door of a house a block away," notes Liebhaber. (sciencedaily.com)
  • Studies in human monocytes are ongoing and will be reported accordingly. (immuneering.com)
  • By establishing the appropriate technical tools, we are moving from an era in which such questions have been asked by studying individual genes to one in which large domains, whole chromosomes, and the entire human genome can be investigated. (ox.ac.uk)
  • We show that a human cell's transcriptome can be divided into active genes carrying out the work of the cell and other genes that are likely the by-products of biological or experimental noise. (biomedcentral.com)
  • We conduct studies of early human brain development. (ncl.ac.uk)
  • Myometrium from the lower uterine segment (LUS) had greater expression of EP2 receptor mRNA and less expression of EP3 mRNA compared with the fundus and corpus. (gla.ac.uk)
  • Expression of EP2, EP3 and EP4 receptor mRNA were also detected in cervix, decidua and chorion. (gla.ac.uk)
  • The variation in myometrial expression of genes encoding EP receptor sub-types paralleled the contractile responses of paired samples (reported elsewhere). (gla.ac.uk)
  • Labor was associated with a reduction in the regional variation of both EP2 and IP receptor gene expression, but not EP1 and EP3 expression. (gla.ac.uk)
  • Labor was also associated with an overall lower level of expression of EP2 receptor mRNA. (gla.ac.uk)
  • Expression of the EP2, FP and TP receptor genes was much lower in animals that were delivered during spontaneous labor than in animals which were not in labor. (gla.ac.uk)
  • 6 When expression of prostanoid receptor genes was studied in decidua and chorion obtained from animals both in labour and not in labour, expression of the genes encoding the EP1 and FP receptor in decidua and the EP4 receptor in chorion was lower with advancing gestational age. (gla.ac.uk)
  • receptor gene was lower in labour in decidua, whereas expression of the IP receptor gene was higher in labour in both decidua (2-fold) and chorion (4-fold). (gla.ac.uk)
  • 1994. Ah receptor in embryonic mouse palate and effects of TCDD on receptor expression. (cdc.gov)
  • We conclude that it may be important to define prognostic expression profiles separately for estrogen receptor--positive and estrogen receptor-- negative tumors. (lu.se)
  • from which they extracted 70 that were used to predict recurrences within 5 years with an accuracy of 89% for a Estrogen receptor- and gene expression in test set of 20 tumors. (lu.se)
  • Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. (who.int)
  • Finally, the combination of limb somite and lateral plate electroporation can provide us with a tool to study the molecular and cellular interactions between the different components of the musculoskeletal system. (biologists.com)
  • The host-pathogen interactions that define a disease are clearly complex, and, in many cases, the study of these interactions is limited by the lack of a suitable animal model. (cdc.gov)
  • Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
  • Disrupted protein interactions identify gene sets involved in risk for ASD. (springer.com)
  • Collectively, these studies demonstrate that inhibition of AREG reduced, whereas AREG supplementation promoted, the airway inflammatory recovery response following environmental bioaerosol exposure, and AREG enhanced fibroblast function, suggesting that AREG could be targeted in agricultur al workers repetitively exposed to organic dust environments to potentially prevent and/ or reduce disease. (cdc.gov)
  • Work in our department has developed a rapid evolution computational protocol whereby the fitness of thousands of mutations of any given gene can be assessed simultaneously. (umassmed.edu)