• However, a key discovery was that genes encoding proteins involved in interaction with the host coagulation/fibrinolysis system and bacterial-host interactions were rapidly up-regulated. (nih.gov)
  • Extensive transcript changes also occurred for genes involved in carbohydrate metabolism, including multi-functional proteins and regulators putatively involved in pathogenesis. (nih.gov)
  • and genes encoding proteins implicated in binding/activation of plasminogen: gbs0608 (eno), gbs1811 (gapC), and gbs1195 (ska). (nih.gov)
  • Importantly, we observed up-regulation of four genes encoding LPXTG proteins after 30 min of incubation in blood, including gbs0428, gbs1087 (fbsA) encoding a major receptor for fibrinogen, gbs1420 (bsp) which encodes a putative choline-binding protein, and gbs2018 (bibA) which encodes a protein that binds to human C4-binding protein and contributes to survival in human blood (Fig. 4A and Table 2). (nih.gov)
  • The mRNA levels of several aggregated proteins of the yajL mutant were severely affected, suggesting that YajL also acts at the level of gene expression. (ecoliwiki.net)
  • Examination of cellular proteins in stpA, hns, or stpA-hns backgrounds revealed that StpA can repress and activate a subset of H-NS-regulated genes. (mblwhoilibrary.org)
  • Mechanistic parallels in regulation of gene expression are indicated by the ability of both proteins to inhibit transcription from promoters containing curved DNA sequences, and to form nucleoprotein structures that constrain DNA supercoils. (mblwhoilibrary.org)
  • At this moment he is Associate Professor in Medical Microbiology, head of the Laboratory for Experimental Bacteriology and head of the Netherlands Reference Laboratory for Bacterial Meningitis (all at the Dept. of Medical Microbiology, Academic Medical Centre, University of Amsterdam). (amc.nl)
  • Importantly, our findings demonstrate for the first time that recombinant E. coli communicate the stress or burden of overexpressing heterologous genes through the quorum-based AI-2 signaling pathway. (nih.gov)
  • Generally, in these mechanisms, a transcription terminator is located between a promoter and a downstream gene(s), and the efficiency of the terminator is controlled by a regulatory effector that can be a metabolite, protein, or RNA. (asm.org)
  • However, NMR studies show that in the DNA-bound state, two domains tumble separately and an REC-DBD interaction is transiently populated in solution.Reporter gene analyses of PmrA variants with altered interface residues suggest that the interface is not crucial for supporting gene expression.We propose that REC-DBD interdomain dynamics and the DBD-DBD interface help PmrA interact with RNA polymerase holoenzyme to activate downstream gene transcription. (nih.gov)
  • We propose that REC-DBD interdomain dynamics and the DBD-DBD interface help PmrA interact with RNA polymerase holoenzyme to activate downstream gene transcription. (nih.gov)
  • en] The production of beta-lactamase in Streptomyces cacaoi, which contains two beta-lactamase-encoding genes, blaL and blaU, is inducible by beta-lactam compounds. (uliege.be)
  • We therefore propose that in addition to its role as a molecular back-up of H-NS, StpA's superior effect on RNA may be exploited under some specific cellular conditions to promote differential gene expression. (mblwhoilibrary.org)
  • Analysis of stpA and hns expression indicated that although stpA transcript levels are much lower than those of hns, the two gene products are capable of both negative autogenous control and cross-regulation. (mblwhoilibrary.org)
  • Consequently, the new KS based method proposed here, which assigns P -values to fixed length upstream regions, provides a fast and accurate approach for predicting bacterial transcription targets. (frontiersin.org)
  • Biotechnological doxorubicin production: pathway and regulation engineering of strains for enhanced production. (nih.gov)
  • The aidB gene encodes a protein of ca. 60 kDa that is homologous to several mammalian acyl coenzyme A dehydrogenases. (umassmed.edu)
  • Ada protein plays a direct role in aidB regulation: methylated Ada is able to bind to the aidB promoter region and to activate transcription from aidB in an in vitro transcription-translation system using crude E. coli extracts. (umassmed.edu)
  • The protein aggregation phenotype of the yajL mutant, which can be rescued by the wild-type yajL gene, but not by the corresponding cysteine 106 mutant allele, is similar to that of multiple mutants deficient in superoxide dismutases and catalases, although intracellular hydrogen peroxide levels were not increased in the yajL mutant, suggesting that protein aggregation in this strain does not result from a hydrogen peroxide detoxification defect. (ecoliwiki.net)
  • The two beta-lactamase genes of Streptomyces cacaoi, blaL and blaU, are under the control of the same regulatory system. (uliege.be)
  • This approach is implemented for pleiotropic bacterial regulators, including σ 70 (bacterial housekeeping σ factor) target predictions, which is a classical bioinformatics problem characterized by low specificity. (frontiersin.org)
  • We are particularly interested in the bacterial stress response and its regulation by small RNAs (ribo-regulation) in conjunction with alternative sigma factors like sigmaE. (amc.nl)
  • PmrA, an OmpR/PhoB family response regulator, manages genes for antibiotic resistance. (nih.gov)
  • In collaborative studies with Dr. D. van de Beek (Department of Neurology, AMC) we investigate clinical aspects of bacterial meningitis as well correlation between clinical features, outcome and host and bacterial factors. (amc.nl)
  • In Bordetella species, the BvgAS system represses and activates sets of genes, and mediates a biphasic phenotypic transition. (umassmed.edu)
  • Another T-N11-A motif upstream of the blaU gene was also shown to bind BlaA. (uliege.be)
  • A plasmid bearing the blaU structural gene and the blaA-blaB regulatory region was constructed and shown to confer on an S. lividans host the capacity to produce inducible beta-lactamase. (uliege.be)
  • We show that growth rate can be used to predict the unregulated expression baseline of a gene, since growth rate dependence of global regulation occurs both in steady state and during transient changes in growth rate. (embopress.org)