Gene expression regulating the expression micro rna that regulates. Medical search. Frequent questions

Recent genome-wide molecular genetic studies have identified several protein-coding genes and microRNAs (miRNAs) significantly associated with BD. (en-journal.org)
Together, our results provide new insights into the potential role of miR-1908-5p in the pathogenesis of BD and also propose a hypothesis that neuronal synapses could be a key converging pathway of some BD-associated protein-coding genes and miRNAs. (en-journal.org)
A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
Furthermore, the miRNAs correlated negatively with the immunohistochemical expression of NRF1 and NRF2 but positively with their respective mRNA. (hindawi.com)
MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression by imperfect matching of mRNA [ 6 ]. (hindawi.com)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
It seemed possible that these genes are regulated by transcription factors that are themselves regulated by miRNAs, and at a translational level by a process involving DRB2. (nature.com)
MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. (frontiersin.org)
The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)
MicroRNAs (miRNAs) are short (21-24 nucleotides) sequences of RNA that reduce gene expression by promoting the breakdown of messenger RNAs (mRNAs) and by repressing translation of mRNAs into proteins. (freethoughtblogs.com)
We show that, similar to metazoan steady-state systems, endogenous miRNAs in Chlamydomonas can regulate gene expression both by destabilization of the mRNA and by translational repression. (freethoughtblogs.com)
We aimed to evaluate the selected miRNAs expression based on bioinformatic analysis and their changes before and after an ultramar. (researchgate.net)
Objective: To identify miRNAs that regulate angiogenesis. (ca.gov)
MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
In our study, we identified miRNAs in hops and their expression patterns in the roots of susceptible and resistant hop cultivars in the early stages of infection with the fungus V. nonalfalfae . (researchsquare.com)
Differentially expressed miRNAs target 49 transcripts and their gene ontology enrichment analysis showed that the susceptible cultivar responds by modulating the processes of protein localization and pigment synthesis, whereas the resistant cultivar responds by modulating transcription factors and hormone signalling. (researchsquare.com)
DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
Based on the consistent expression patterns associated with patient survival outcomes and in tumors vs. normal lung tissues, 10 miRNAs were considered to be putatively tumor suppressive and 4 miRNAs were deemed as oncogenic in lung cancer. (cdc.gov)
From the list of genes that were targeted by the 73 diagnostic miRNAs, DGKE and WDR47 had significant associations with responses to both systemic therapies and radiotherapy in lung cancer. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)

The differentially expressed genes (DEGs) in AD were screened in GSE48350 dataset. (nature.com)
The differentially expressed oxidative stress genes (DEOSGs) were analyzed by intersection of DEGs and oxidative stress-related genes. (nature.com)
The OA subchondral bone dataset GSE51588 was downloaded from the GEO database, and the differentially expressed genes (DEGs) were screened. (spandidos-publications.com)
Core genes were expressed differentially between OA subchondral bone and normal tissue samples. (spandidos-publications.com)
Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
The expression of the following genes was salient: (a) Cd163, an anti-inflammatory stroke-associated gene, was the most differentially expressed gene by NPD1+RvD1, displaying more than a 123-fold upregulation in the ipsilesional penumbra (Lisi et al. (springer.com)
Conclusion and Clinical Relevance: Examination of the pulmonary genetic profiles revealed differentially expressed genes and microRNAs following subchronic inhalation exposure to A. fumigatus. (cdc.gov)

Investigation of hub genes for the development of potential therapeutic targets and candidate biomarkers is warranted. (nature.com)
Circulating extracellular RNAs (ex-RNAs) are an emerging class of biomarkers with target-organ epigenetic effects. (researchgate.net)
Furthermore, altered microRNAs may serve as potential biomarkers to evaluate fungal exposure. (cdc.gov)
How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)

MicroRNAs are tiny ribonucleic acid (RNA) molecules (~22 nucleotides long) that recently have been found to play important roles in regulating gene expression in eukaryotic organisms, including plants and animals. (sciencedaily.com)

A total of 1160 DEGs (259 up-regulated and 901 down-regulated) were screened in GSE48350. (nature.com)

miRNA binding downregulates the expression of target mRNAs either by decreasing their stability or by inhibiting translation. (en-journal.org)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
mRNAs corresponding to several regulatory genes that mediate auxin responses contain short stretches of sequence that are complementary to microRNAs, and therefore have been considered potential targets of microRNA-mediated regulation. (sciencedaily.com)
The plants showed increased accumulation of ARF17 mRNA and altered levels of mRNAs corresponding to several genes that may be regulated by ARF17. (sciencedaily.com)

In animals, the degree of base pairing between a miRNA and its target messenger RNA seems to determine whether the regulation occurs through cleavage or translation inhibition 1 . (nature.com)
Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (sciencedaily.com)
Objective: The impact of fungal viability on the pulmonary microRNA and messenger RNA profiles that regulate murine immune responses was evaluated following subchronic inhalation exposure to Aspergillus fumigatus conidia. (cdc.gov)

Notably, some of the proteins expressed from BD-associated genes function in neuronal synapses, suggesting that abnormalities in synaptic function could be one of the key pathogenic mechanisms of BD. (en-journal.org)
It is a main inductor of genes of antioxidant proteins and phase II detoxifying enzymes [ 1 ]. (hindawi.com)
Immunohistochemical staining was performed to confirm the expression of related proteins in OA and non‑OA tissue samples. (spandidos-publications.com)
Furthermore, our results reveal that the core silencing proteins ARGONAUTE1 (AGO1) and SERRATE (SE) are highly regulated by miRNA-guided translational inhibition. (nature.com)
DRB2 has been remarkably conserved throughout plant evolution, raising the possibility that translational repression is the ancient form of miRNA-directed gene regulation in plants, and that Dicer partnering proteins, such as human TRBP, might play a similar role in other eukaryotic systems. (nature.com)
Research reported in The Plant Cell shows that microRNAs control the accumulation of transcription factor proteins that regulate the expression of genes in the auxin response pathway. (sciencedaily.com)
There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (sciencedaily.com)
Degradation of cellular proteins in the ubiquitin-proteasome system regulates various biological processes. (helsinki.fi)
Their presence in the cytoplasm is directly or indirectly detected by receptors, so-called resistance (R) proteins, or nucleotide-binding leucine-rich repeat (NB-LRR) proteins encoded by R- genes. (researchsquare.com)

However, the components and mechanism(s) that determine whether a plant miRNA ultimately regulates its targets by guiding cleavage or translational inhibition are unknown 6 . (nature.com)
We found that DRB2 determines miRNA-guided translational inhibition and represses DRB1 expression, thereby allowing the active selection of miRNA regulatory action. (nature.com)
The microarray analysis of drb2 also revealed a large set of gene transcripts that were significantly different from wild-type levels, but which are not thought to be the primary targets of miRNA-directed regulation ( Supplementary Fig. 3 ). (nature.com)
Figure 1: Expression patterns of DRB1 and DRB2 in wild-type plants, and miRNA target expression in drb1 and drb2 . (nature.com)
The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
Here, we provide a comprehensive global analysis of the evolutionarily distant unicellular green alga Chlamydomonas reinhardtii to quantify the effects of miRNA on protein synthesis and RNA abundance. (freethoughtblogs.com)
Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)
Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)

Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)

Functional enrichment analysis was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and then Gene Set Enrichment Analysis (GSEA) was used to formulate the molecular functions and pathways based on the results of GO and KEGG analyses. (spandidos-publications.com)
2021 ). Moreover, the resolution of inflammation is not passive as it engages a biosynthetically active process, regulated by specific mediators and receptor-signaling pathways and driven by specialized pro-resolving mediators (SPMs) (Serhan and Petasis 2011 ). (springer.com)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

Auxin influences development by affecting the expression of numerous genes that control the processes of cell division and cell expansion in specific plant tissues at specific stages during the plant life cycle - e.g. for leaves, roots, and floral organs to develop in the correct patterns and correct time sequence. (sciencedaily.com)
They can modulate various biochemical and physiological processes, including plant response and resistance to fungal pathogens, by regulating the expression of numerous genes. (researchsquare.com)

One of these targets is the transcription factor AUXIN RESPONSE FACTOR17 (ARF17), which is thought to repress the expression of a number of other genes involved in auxin responses. (sciencedaily.com)
Although they are structurally similar, plant and animal microRNAs repress gene expression through very different mechanisms. (freethoughtblogs.com)

Finally, we constructed the drug, transcription factors (TFs), and microRNA network of the diagnostic genes. (nature.com)
Our aim was to investigate these redox transcription factors in conjunction with redox-related microRNAs in naevi and melanoma. (hindawi.com)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)
In a PCR array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of signal transducer and activator of transcription 3 (STAT3). (cdc.gov)

Enrichment analysis was performed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. (nature.com)

Twenty-two of the pairs, chromosome numbers 1 to 22, the autosomes, are normally homologous (identical in size, shape, and position and number of genes). (msdmanuals.com)
the smaller Y chromosome carries genes that initiate male sex differentiation, as well as a few other genes. (msdmanuals.com)
The genes that occupy the same locus on each chromosome of a pair (one inherited from the mother and one from the father) are called alleles. (msdmanuals.com)
A chromosome contains many genes. (msdmanuals.com)

Our study identified 9 key genes associated with oxidative stress and immune reaction in AD pathogenesis. (nature.com)
Oxidative stress is involved in the modulation of gene expression patterns and metabolic activities, characterized by the disruption of redox homeostasis 7 . (nature.com)
Similar to NRF2, it is responsive to oxidative stress and activates antioxidant responsive element- (ARE-) driven genes [ 4 ]. (hindawi.com)

We characterized the immunohistochemical expression of NRF1 and NRF2 in 99 naevi, 88 primary skin melanomas, and 67 lymph node metastases. (hindawi.com)

These changes were correlated with dramatic development defects in leaves, roots, and flowers, showing that microRNA-mediated regulation of ARF17 is essential for normal plant development. (sciencedaily.com)
Arabidopsis ago1 mutants lacking the AGO1 protein have numerous severe developmental defects, supporting the notion that regulation by microRNAs is critical for normal plant growth. (sciencedaily.com)
Dr. Bellini and her colleagues found that the mutant plants over-accumulate ARF17 mRNA within the hypocotyl, pointing to ARF17 as a major regulator of adventitious rooting and microRNA-mediated regulation as a major regulator of ARF17. (sciencedaily.com)
We have only known that microRNAs even existed since the early 1990's, and their importance in gene regulation and development wasn't appreciated until the 2000's. (freethoughtblogs.com)
The objective of this study is to identify microRNAs-small, non-coding RNAs that help control gene expression-that contribute to the regulation of new blood vessel formation. (ca.gov)

Research conducted by three independent groups and reported in The Plant Cell shows that fundamental developmental processes controlled by the plant hormone auxin are regulated by microRNAs in the model plant Arabidopsis thaliana. (sciencedaily.com)

The immune-related DEOSGs and hub genes were identified by weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis, respectively. (nature.com)
We obtained 66 immune-related DEOSGs by intersecting turquoise module with the DEOSGs and identified 15 hub genes through PPI analysis. (nature.com)
2021 ). They exert multiple biological functions, including limiting excessive inflammatory responses, regulating metabolism and immune cell functions, decreasing the production of pro-inflammatory factors, increasing anti-inflammatory mediators, and promoting tissue repair and homeostasis (Miao et al. (springer.com)
MicroRNAs regulating genes involved in the pulmonary immune responses were those with the greatest fold change. (cdc.gov)

The mRNA expression of diagnostic genes was determined by qRT-PCR analysis. (nature.com)
Influence of Aspergillus fumigatus conidia viability on murine pulmonary microRNA and mRNA expression following subchronic inhalation exposure. (cdc.gov)

Dual-luciferase reporter gene assays demonstrated Arhgap11a as the target of miR-211-5p. (frontiersin.org)

Analysis from the Gene Expression Omnibus datasets supported the mRNA findings. (hindawi.com)
Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)

Dr. Bonnie Bartel at Rice University in Houston, TX together with Drs. David Bartel and Allison Mallory at the Massachusetts Institute of Technology and the Whitehead Institute for Biomedical Research in Cambridge, MA report experiments using transgenic Arabidopsis plants that produce a version of ARF17 mRNA that resists microRNA-mediated cleavage. (sciencedaily.com)
This result implies that the Arabidopsis embryo relies on microRNA restriction of ARF17 activity to achieve normal bilateral symmetry. (sciencedaily.com)
A second report focuses on the function of the protein ARGONAUTE1 (AGO1), a major component of the RNA-induced silencing complex in Arabidopsis. (sciencedaily.com)

Developmental genes, especially Hox genes, turned out to be remarkably conserved across lineages that diverged over half a billion years ago. (freethoughtblogs.com)

PTI comprises both physical and chemical defence responses, e.g. the deposition of lignin-like compounds in the cell wall, the production of reactive oxygen species (ROS) and the activation of signalling cascades that modulate gene expression. (researchsquare.com)

It is generally hypothesized to be related to aging, trauma, inflammation, obesity, metabolism and genetics, and may also be related to genetic factors, chromosomal abnormalities and gene fusion ( 5 , 6 ). (spandidos-publications.com)
Genetic experiments in zebrafish revealed that miR-10 functions, in part, by directly regulating the level of a protein called FLT1, which inhibits the behavior of endothelial cells that promotes new blood vessel growth. (ca.gov)

Results: Comparison of datasets revealed increased Il4, Il13, and Il33 expression in mice exposed to viable versus heat-inactivated conidia. (cdc.gov)

Scope includes mutations and abnormal protein expression. (cancerindex.org)
It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)

Methods and Results: We show that the highly conserved microRNA family encoding miR-10 regulates the behavior of endothelial cells during angiogenesis by positively titrating pro-angiogenic signaling. (ca.gov)

The mRNA levels in a cell are fine tuned by different mechanisms, one of which is driven by microRNA molecules. (sciencedaily.com)
MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (sciencedaily.com)
Within the complex, microRNAs are thought to bind to mRNA molecules containing a complementary stretch of RNA sequence. (sciencedaily.com)
Micro RNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. (researchsquare.com)

Comparative Toxicogenomics Database and TargetScan were used to identify the hub‑gene‑related diseases and the microRNAs that regulated the central hub genes. (spandidos-publications.com)
The ability to regulate blood vessel growth is important for treating many diseases, including cancer. (ca.gov)
Accumulating evidences have elucidated connection between the expression of GTPases members and several diseases, including colorectal cancer, lung cancer, and prostate cancer [ 16 - 18 ]. (hindawi.com)

Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. (researchgate.net)

Moreover, bioinformatic analyses of human brain expression profiles revealed that the expression levels of miR-1908-5p and its synaptic target genes show an inverse-correlation in many brain regions. (en-journal.org)

The subsequent huge changes in morphology were more often due to changes in when and where those genes were expressed than to changes in the coding sequences of the genes themselves. (freethoughtblogs.com)

The diagnostic value of hub genes was assessed by receiver operating characteristic analysis and validated in GSE1297. (nature.com)
In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)

I am molecular and cellular biologist with expertise in virus and microRNAs. (researchgate.net)
8 Howard Hughes Medical Institute and Laboratory for RNA Molecular Biology, The Rockefeller University, New York, New York, USA. (jci.org)

Altered expression of circular RNAs (circRNAs) has previously been investigated in breast cancer. (researchgate.net)

We identified and validated its novel target genes including DLGAP4 , GRIN1 , STX1A , CLSTN1 and GRM4 , which all function in neuronal glutamatergic synapses. (en-journal.org)
As a consequence, both factors are transported to the nucleus to induce the expression of their target genes. (hindawi.com)
In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

We found that miR-10 functions, in part, by directly regulating the level of fms-related tyrosine kinase 1 (FLT1), a cell-surface protein that sequesters VEGF, and its soluble splice variant sFLT1. (ca.gov)

The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
Osterix (OSX) is a transcription factor in the SP family, which is essential for osteoblast differentiation, and its expression is closely related to Runx2 ( 9 ). (frontiersin.org)

In our preliminary experiments, the expression of miR-1908-5p was increased after chronic treatment with valproate but not lithium in control human neural progenitor cells. (en-journal.org)

To achieve the best effectiveness of the defence response, all defence mechanisms must be well regulated. (researchsquare.com)

One of the biggest changes in evolutionary theory in the late 20th century was the growing appreciation for the central role of changes in gene expression in macroevolution. (freethoughtblogs.com)

Weighted gene co‑expression network analysis was performed, and a protein‑protein interaction network was constructed and further analyzed using Cytoscape and STRING. (spandidos-publications.com)
Total RNA was isolated from whole lung 24 and 48 hours post final exposure and was further processed for gene expression and microRNA array analysis. (cdc.gov)
HBEpCs overexpressing DEFB1 caused a significant reduction in IAV, that was confirmed by IAV matrix gene analysis, plaque assay, and confocal microscopy. (cdc.gov)

Anatomy7

Organisms2

Chemicals and Drugs35

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes40

Disciplines and Occupations1

Information Science3

Health Care1

MiRNAs23

Differentially7

Biomarkers4

Nucleotides1

DEGs1

MRNAs4

Messenger RNA4

Proteins9

MiRNA8

Tumor1

Pathways3

Numerous genes2

Repress2

Transcription factors5

Ontology1

Chromosome4

Oxidative stress3

Immunohistochemical1

Regulation5

Developmental processes1

Immune4

MRNA expression2

Assays1

Omnibus3

Arabidopsis3

Remarkably conserved1

Responses1

Genetic2

Datasets1

Protein expression3

Angiogenesis1

Molecules4

Diseases3

Small1

Profiles revealed1

Sequences1

Diagnostic2

Molecular2

Breast Cancer1

Target3

Functions1

Differentiation2

Control1

Mechanisms1

Evolutionary1

Analysis3