• In the field of molecular biology , gene expression profiling is the measurement of the activity (the expression ) of thousands of genes at once, to create a global picture of cellular function. (wikipedia.org)
  • At the facility, researchers will use image analysis technology and molecular biology processes to generate gene expression data for drug and drug target discovery, the company said. (genomeweb.com)
  • Gerami and colleagues (2015a) assessed the prognostic accuracy of gene expression profiling for molecular staging of cutaneous melanoma in a multicenter cohort study of 217 individuals undergoing sentinel lymph node biopsy (SLNB). (unicare.com)
  • To better understand the molecular mechanisms underlying this transformation, we performed a comparative analysis using gene expression profiling of A2B5 + oligodendrocyte progenitors and O4 + oligodendrocytes. (jneurosci.org)
  • This initiative, which provides a natural complement to Foundation Medicine's comprehensive genomic profiling (CGP) suite of molecular information tests, further enhances support for the Company's biopharma partners in the efficient identification of known and novel genomic and expression-based biomarkers of response for investigational and approved personalized cancer therapies, including new and existing cancer immunotherapies. (businesswire.com)
  • The company offers a full suite of comprehensive genomic profiling assays to identify the molecular alterations in a patient's cancer and match them with relevant targeted therapies, immunotherapies and clinical trials. (businesswire.com)
  • Gene expression profiling is a technique used in molecular biology to query the expression of thousands of genes simultaneously. (wikipedia.org)
  • The unique pattern of gene expression for a given cell or tissue is referred to as its molecular signature. (wikipedia.org)
  • Different molecular subgroups within PTCL unspecified have been identified associated to different expression profiles. (wiley.com)
  • In this study, we use gene expression profiling (GEP) to characterize the molecular profile of H-MM, with a view of gaining some insights about its biology, and to study the heterogeneity within H-MM. (aacrjournals.org)
  • Molecular classification of human carcinomas by use of gene expression signatures. (springer.com)
  • This three-day meeting will address key issues concerning genetics, genotype profiling and gene expressions in the broader context of molecular biology, evolutionary biology, bioinformatics, Genomics and Case studies. (ourglocal.com)
  • METHODOLOGY/PRINCIPAL FINDINGS: To identify genes and molecular functions transcriptionally affected by gamma-secretase activity, the cellular transcriptomes of Chinese hamster ovary (CHO) cells with enhanced and inhibited gamma-secretase activity were analyzed and compared by cDNA microarray. (epfl.ch)
  • CONCLUSIONS/SIGNIFICANCE: Investigating the effects of gamma-secretase activity on gene transcription has revealed several affected clusters of molecular functions and, more specifically, 21 genes that hold significant potential for a better understanding of the biology of gamma-secretase and its roles in cancer and Alzheimer's disease pathology. (epfl.ch)
  • FRIENDSWOOD, Texas--( BUSINESS WIRE )--Castle Biosciences, Inc., a provider of molecular diagnostics to improve cancer treatment, today announced results of an independent study with its noninvasive gene expression profile test, verifying the accuracy and utility of the assay to identify risk of metastasis in patients diagnosed with Stage I or II cutaneous melanoma. (businesswire.com)
  • As only some smokers develop COPD with emphysema, we explored the molecular pathogenesis of early-stage COPD with emphysema using gene expression profiling of human lung tissues. (dovepress.com)
  • Ten muscle samples from LGMD2A patients with in which molecular diagnosis was ascertained were investigated using array technology to analyze gene expression profiling as compared to ten normal muscle samples. (upm.es)
  • This project will further examine the physiological (i.e. sperm counts and quality) and molecular (i.e. testicular and epididymal gene expression profiles) effects of developmental exposure to DES and investigate the effects of genistein (GEN) and o,p'-dichlorodiphenyltrichlorothane (o,p'-DDT), two environmentally relevant, albeit weak estrogenic endocrine disruptors. (epa.gov)
  • For the purposes of risk assessment, elucidating correlations between physiological effects and changes at the molecular level (i.e. modulation of gene expression profiles) is critically important in order to establish potential mechanisms of action and to confirm that changes in the expression of gene networks translate into the manifestation of a toxic response. (epa.gov)
  • In this study, we report for the first time a whole genome transcriptional profile analysis of the human monocyte-to-macrophage differentiation and polarized activation processes, describing distinct molecular signatures which shed new light on these processes and reveal new candidate markers. (jimmunol.org)
  • Candida albicans Transcriptional Profiling Within Biliary Fluid From a Patient With Cholangitis, Before and After Antifungal Treatment and Surgical Drainage. (jcvi.org)
  • Although many genes involved in learning and memory formation have been identified, little is known about the genetic mechanisms required for changing the transcriptional program during different phases of long-term memory (LTM) formation. (genetics.org)
  • I: Uncovering a macrophage transcriptional program by integrating evidence from motif scanning and expression dynamics. (psu.edu)
  • Single genes with the most pronounced transcriptional susceptibility to gamma-secretase activity were evaluated by real-time PCR. (epfl.ch)
  • For instance, skin cells, liver cells and nerve cells turn on (express) somewhat different genes and that is in large part what makes them different. (wikipedia.org)
  • Gene expression profiling tests (Oncotype DX, MammaPrint, others) analyze a number of different genes within your cancer cells to predict your risk of cancer recurrence. (mayoclinic.org)
  • A total of 6878 cDNAs were analyzed in this study, estimated to represent ∼5500 different genes (perhaps ∼40% of genes in the honey bee genome) ( 17 , 18 ). (sciencemag.org)
  • BACKGROUND: Processing by gamma-secretase of many type-I membrane protein substrates triggers signaling cascades by releasing intracellular domains (ICDs) that, following nuclear translocation, modulate the transcription of different genes regulating a diverse array of cellular and biological processes. (epfl.ch)
  • We investigated global gene expression profiles from HCC arising in different liver diseases to test whether HCC development is driven by expression of common or different genes, which could provide new diagnostic markers or therapeutic targets. (pubmedcentralcanada.ca)
  • In response to a cellular perturbation, changes in gene expression take place that result in the expression of hundreds of gene products and the suppression of others. (uptodate.com)
  • Understanding the changes in gene expression in fishes exposed to hypoxic stress could reveal novel mechanisms of tolerance that may shed new light on hypoxia and ischemia in higher vertebrates. (pnas.org)
  • Microarray studies have been used to relate changes in behavior with changes in gene expression in the brain ( 3 - 7 ). (sciencemag.org)
  • Together, our results provide first evidence for a specific effect of EE on T cell differentiation and its associated changes in gene expression profile. (frontiersin.org)
  • The General Linear Model (GLM) will be used as a novel statistical approach to discern dose-dependent and temporal associations between effects on sperm quality and changes in gene expression profiles. (epa.gov)
  • Moreover, analyzing chronological changes in gene expression of cerebral artery may be helpful for elucidating the mechanism of cerebral vasospasm because the delayed onset is a fundamental feature of the pathophysiology of cerebral vasospasm. (omicsonline.org)
  • Sequence based techniques, like RNA-Seq , provide information on the sequences of genes in addition to their expression level. (wikipedia.org)
  • the sequence tells us what the cell could possibly do, while the expression profile tells us what it is actually doing at a point in time. (wikipedia.org)
  • d) for each distinctly sized amplification product detected in step (c), calculating a cycle number, C t , where the amount of that amplification product crosses a predefined threshold, and correlating the threshold cycle with the amount of a nucleic acid having a sequence of interest in said sample, wherein said method provides an amplification profile and a relative abundance for members of said set of nucleic acid sequences of interest. (google.ca)
  • Supplementary Table 1-Primer sequence of selected genes designed for qRT-PCR. (springer.com)
  • To date, however, microarray analyses have been applied almost exclusively to model species for which gene sequence data are abundant. (pnas.org)
  • We show the utility of microarray approaches for the study of gene expression in a species for which, at the onset of our investigation, sequence data were unavailable. (pnas.org)
  • Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling . (primidi.com)
  • The level of fluorescence at a particular spot provides quantitative information about the expression of the particular gene corresponding to the spotted cDNA sequence. (wikipedia.org)
  • First Monoploid Reference Sequence of Sugarcane For the highly polyploid sugarcane, an international team of researchers has successfully assembled a first monoploid reference sequence using a targeted approach that focused on the gene rich part of the genome by harnessing information from a sequenced related species - sorghum. (doe.gov)
  • The first step in gene discovery was to establish a complementary DNA (cDNA) library and a database of expressed sequence tags (ESTs) for P. multiseries. (mit.edu)
  • Several genes that may be involved in domoic acid synthesis were also revealed through sequence similarity, for example, glutamate dehydrogenase and 5-oxo-L-prolinase. (mit.edu)
  • 0.1 with respect to null adenovirus) changes in the expression of 1,238 genes or expressed sequence tags, of which 1,180 (95.3%) were upregulated. (diabetesjournals.org)
  • However, resolving expression of closely related genes (e.g. alleles and gene family members) is challenging on a genome-wide scale due to extensive sequence similarity and frequently incomplete genome sequence data. (plantphysiol.org)
  • Genes with extensive sequence similarity may comprise a significant portion of a given transcriptome. (plantphysiol.org)
  • The extent of these sequence similarities in maize and other complex genomes poses a clear challenge to delineation of gene-specific function. (plantphysiol.org)
  • a growing number of chemosensory genes have also been identified from many other Dipteran species based on sequence similarity. (frontiersin.org)
  • However, our ability to predict gene function from DNA sequence is poor. (biologists.org)
  • This study involves a comprehensive, multi-modal, and integrative assessment of biomarkers implicated in the pathophysiology of PTSD, including measuring differences in whole-blood gene expression and other blood biomarkers of key neurobiological systems, an approach critical to informing risk and resilience prediction algorithms for PTSD, and to develop novel psychopharmacologic approaches for the treatment of this disabling condition in disaster responders and other trauma survivors. (cdc.gov)
  • The aim of this study was to identify genes that are expressed differently in the course of BC progression and to establish new biomarkers for BC. (sigmaaldrich.com)
  • We identified several genes as promising candidates for diagnostic biomarkers of human BC and the CKS2 gene not only as a potential biomarker for diagnosing, but also for staging human BC. (sigmaaldrich.com)
  • Moreover, results from these studies may identify novel gene targets that are involved in the toxicity of endocrine disruptors that could lead to the development of biomarkers for reproductive toxicants. (epa.gov)
  • However, nine acid-inducible genes are clustered in the gadA region, including hdeA , which encodes a putative periplasmic chaperone, and four putative regulatory genes. (asm.org)
  • To identify temperature-responsive genes, genome arrays containing 1,662 putative B. burgdorferi open reading frames (ORFs) were prepared on nylon membranes and employed to assess gene expression in B. burgdorferi B31 grown at 23 and 35 degrees C. Differences in expression of more than 3.5 orders of magnitude could be readily discerned and quantitated. (prohealth.com)
  • With no hypothesis, there is nothing to disprove, but expression profiling can help to identify a candidate hypothesis for future experiments. (wikipedia.org)
  • Together, our results highlight the usefulness of this discovery-driven experimental strategy to identify genes relevant to oligodendrocyte differentiation and myelination. (jneurosci.org)
  • One potentially useful approach to solve these issues would be to identify specific gene expression changes in cancerous mesothelial cells. (aacrjournals.org)
  • and (2) to identify additional genes that discriminate IS from vascular risk factor ( S ex, A ge and V ariation in V ascular functionalit Y [SAVVY]) control subjects and myocardial infarction (MI) control subjects. (ahajournals.org)
  • Mapping Heat Resistance in Yeasts In a proof-of-concept study, researchers demonstrated that a new genetic mapping strategy called RH-Seq can identify genes that promote heat resistance in the yeast Saccharomyces cerevisiae, allowing this species to grow better than its closest relative S. paradoxus at high temperatures. (doe.gov)
  • Therefore, the focus of this thesis was to identify and initiate characterization of actively expressed genes that control cell growth and physiology in P. multiseries, with the specific goal of identifying genes that may play a significant role in toxin production. (mit.edu)
  • In this study, we sequenced the antennal transcriptome of H. illucens adults to identify chemosensory genes. (frontiersin.org)
  • Here, we analyzed genes differentially expressed in each of the three LMS subtypes as compared to benign leiomyomas and then used the Connectivity Map (cmap) to calculate enrichment scores for the 1309 cmap drugs in order to identify candidate molecules with the potential to induce a benign, leiomyoma-like phenotype in LMS cells. (harvard.edu)
  • Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. (nih.gov)
  • Four gene signatures whose expression changed relative to baseline (before wound induction by the pre-treatment biopsy) were identified. (nih.gov)
  • Two additional signatures were observed which included preferentially pro-inflammatory genes in the early post-treatment biopsies (2 days after pre-treatment biopsies) and repair and angiogenesis genes in the later (4 to 8 days) biopsies. (nih.gov)
  • Thus, transcriptome profiling reveals novel molecules and signatures associated with human monocyte-to-macrophage differentiation and polarized activation which may represent candidate targets in pathophysiology. (jimmunol.org)
  • Gene expression profiling revealed that in DLBCL patients who do not respond well to standard chemotherapy, lymphoma cells have activated this pathway, known as NF-kB. (innovations-report.com)
  • In addition, the expression level of NF-kB pathway genes allowed to differentiate two PTCL subgroups, and this difference could have clinical interest. (wiley.com)
  • There are seven genetic subgroups of XP, which are all resultant of pathogenic mutations in genes in the nucleotide excision repair (NER) pathway and a XP variant resultant of a mutation in translesion synthesis, POLH. (mdpi.com)
  • In this webinar, a workflow will be presented for the processing of 384 cell lysates with RNA-seq to generate expression data analyzed at the pathway level. (xtalks.com)
  • We revealed both genes and pathway potentially involved in the pathophysiology of migraine. (biorxiv.org)
  • This is the first study to define Candida global gene expression during deep-seated human infection. (jcvi.org)
  • Single-cell global gene expression profiling showed that undifferentiated cells fell into two clusters delineated by expression of DLL1 and its binding partner syntenin. (biologists.org)
  • Global gene expression profiling was performed for 4 normal (control) livers as well as 8 background liver and 7 HCC from 3 patients with hereditary haemochromatosis (HH) undergoing surgery. (pubmedcentralcanada.ca)
  • For this reason alone, it is clear that additional genes must participate in the glutamate-dependent AR, but these genes remain to be identified ( 9 ). (asm.org)
  • Additional genes that could be important in our understanding of the pathogenesis of mesothelioma, aiding in diagnosis, or improving targets for therapy were also identified. (aacrjournals.org)
  • An average of 4753 and 5965 additional genes was called Present after globin depletion for Donor 37 and Donor 45, respectively. (thermofisher.com)
  • Several gene expression profiling tests exist, and many are being studied in clinical trials. (mayoclinic.org)
  • This document addresses gene expression profiling to assist in the risk stratification and clinical management of cutaneous and uveal (ocular) melanoma. (unicare.com)
  • CAMBRIDGE, Mass.--( BUSINESS WIRE )-- Foundation Medicine, Inc. (NASDAQ:FMI) today announced a comprehensive gene expression profiling (GEP) program to support precision oncology clinical research and development. (businesswire.com)
  • and the benefits of Foundation Medicine's gene expression profiling program to biopharma partners, including optimizing clinical research development programs and drug development. (businesswire.com)
  • The information derived from gene expression profiling often helps in predicting the patient's clinical outcome. (wikipedia.org)
  • The objectives of this study are to compare the gene expression profiles of retinoblastoma, retinocytoma, and normal retina using microarray data and to determine the expression profiles that correlate with clinical and histologic features. (arvojournals.org)
  • These profiles represent further refinement of gene expression as a diagnostic tool in patients with acute IS, which could be used to aid in the diagnosis of stroke in the context of clinical information and evaluation. (ahajournals.org)
  • Gene expression profiling can also be used to predict clinical outcome and response to specific therapeutic agents. (boomerangbooks.com.au)
  • Gene expression profile was measured in 86 of 162 OPL patients who were enrolled in a clinical chemoprevention trial that used the incidence of oral cancer development as a prespecified endpoint. (aacrjournals.org)
  • Our study provides a comprehensive picture of the gene expression landscape in hepatocytes grown on different substrate stiffness, offering insights into the role of substrate stiffness in hepatic pathology. (springer.com)
  • One recent study found that chemotherapy might not be helpful for women with gene expression profiling test results that indicate an intermediate risk of recurrence. (mayoclinic.org)
  • Additional study is need in a randomized sample of individuals to determine how gene expression profiling combined with SLNB would contribute to the accurate staging and treatment planning of individuals with cutaneous melanoma. (unicare.com)
  • The largest group was comprised predominantly of inflammatory genes whose expression was increased throughout the study. (nih.gov)
  • The fourth and smallest set of genes was down-regulated throughout the study. (nih.gov)
  • We're able to reliably predict the survival of these patients using data from a small number of genes, indicating that this technique should be entirely manageable for routine use," said National Cancer Institute (NCI) investigator Louis M. Staudt, M.D, Ph.D., the senior author on the study. (innovations-report.com)
  • For this study, researchers used the Lymphochip, a specialized microarray containing 12,000 DNA spots representing genes expressed in normal and malignant lymphoid cells. (innovations-report.com)
  • In a previous study, we proposed a gene regulatory network (GRN) involved in the formation and regeneration of conspicuous filamentous elongations adorning the unpaired fins of the Neolamprologus brichardi. (diva-portal.org)
  • Our study provided evidence for differences in the anatomy of fin elongation and suggested gene regulatory divergence between the two cichlid species. (diva-portal.org)
  • Fifty-three H-MM and 37 NH-MM with adequately good quality RNA for gene expression study were included. (aacrjournals.org)
  • Using this and other modifications, they completed a study of gene expression in ovarian cancer in a single experiment. (genomenewsnetwork.org)
  • The presented transcriptomic data may further be used as resource to study genes acting at different memory phases. (genetics.org)
  • 6 This profile required validation in a second cohort, which has been done in the current study. (ahajournals.org)
  • Nevertheless, some of the genes identified as SREBP targets in the liver study, including fatty acid synthase and acetyl-CoA carboxylase, were also upregulated by SREBP1c in β-cells ( 3 , 4 ) and in islets ( 6 ), suggesting that this may represent a useful approach to understanding the mechanisms by which SREBP1c overexpression inhibits insulin secretion from islets. (diabetesjournals.org)
  • The study, titled "Estimation of Prognosis in Invasive Melanoma Using a Gene Expression Profile Test" is an independent, single-center performance study of 257 successfully tested, consecutively consented patients, some of whom were previously diagnosed. (businesswire.com)
  • The study objective was to determine the value of gene expression profiling in predicting oral cancer development. (aacrjournals.org)
  • To systematically study the genes associated with risk of OSCC, we used gene expression profiling on a large cohort of samples of OPL patients. (aacrjournals.org)
  • The long-term objective of this study is to elucidate how developmental exposure to estrogenic substances modulate gene expression profiles, leading to compromised male reproductive fitness at later stages of life. (epa.gov)
  • Results: In this study, we first identified 2223 differentially expressed genes by comparing gene expression profiles for two control, two adenoma and two carcinoma samples using an F-test. (sciweavers.org)
  • This study aimed to validate by qPCR the expression of 26 genes, revealed as differentially expressed by other studies, using peripheral blood cells (PBC) of 11 FRDA patients compared to 11 healthy controls. (curefa.org)
  • Through gene expression profiling, researchers are working to find ways to monitor brain health and predict future strokes, aneurysms, and hemorrhages. (ucdavis.edu)
  • If all of the standard factors that doctors use to predict the chance of your cancer returning show that your risk is very small, then gene expression profiling tests probably aren't necessary. (mayoclinic.org)
  • From these genes, the investigators created a formula that could be used to predict survival following chemotherapy. (innovations-report.com)
  • To predict behavior from gene expression profiles in a natural context would demonstrate a more robust relation between genes and behavior than is commonly thought to exist ( 9 ). (sciencemag.org)
  • Our results indicate that surviving 3-day ADX granule cells display lower membrane capacitance, lower relative N-methyl-d-aspartate (NMDA) R1 mRNA expression and higher relative mineralocorticoid receptor (MR), alpha1A voltage-gated Ca-channel, Bcl-2 and NMDA R2C mRNA expression. (uva.nl)
  • The genetic basis for disease is determined by the inheritance of genes containing specific sequences of deoxyribonucleic acid (DNA). (uptodate.com)
  • Traces from 1:20 dilution of one representative whole blood aRNA sample and duplicate non-diluted GLOBINclear Kit samples were overlaid to observe the effects of globin mRNA depletion on aRNA profile. (thermofisher.com)
  • HIF-1α mediates the expression of a series of genes involved in both cellular and systemic responses to hypoxia, leading to enhanced anaerobic metabolism and induced erythropoiesis and angiogenesis ( 2 ). (pnas.org)
  • Diurnally regulated genes in the prefrontal cortex may be controlled by the circadian system, by sleep:wake states, or by cellular metabolism or environmental responses. (umd.edu)