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  • regulate
  • Seems to regulate expression and localization of ITGA3 by transporting it from the nucleus to cytoplasm at adhesion plaques. (uniprot.org)
  • For example, fluorescence labeling of transcription factors demonstrated that while some transcription factors are locally translated in dendrites and travel back to the nucleus, others operate at shorter time scales in the nucleus by increased binding to specific genomic locations, and thereby regulate activity-dependent transcriptional responses. (frontiersin.org)
  • Abstract
  • article{6939d08a-7431-491e-9f57-0be6bd919af5, abstract = {The BCR gene is implicated in the development of Ph-positive leukemia through its fusion with the nonreceptor tyrosine kinase gene ABL. (lu.se)
  • pathways
  • RNA sequencing analysis identified gene expression changes within several key pathways, including Notch and smooth muscle cell contractility. (jci.org)
  • Microarray and next generation sequencing approaches are being used to investigate developmental, physiological and stress response processes, dissect epigenetic and small RNA pathways, and build large gene regulatory networks 1-3 . (jove.com)
  • Dysregulation of these pathways can lead to aberrant gene expression patterns and has been associated with a number of neurodevelopmental and neuropsychiatric impairments like dementia, autism spectrum disorders, and mental retardation. (frontiersin.org)
  • To this end, fluorescence imaging, including optogenetic manipulation of signaling pathways, provides a tremendous boost in the field of molecular neuroscience, whereby we are now beginning to understand the molecular interplay in the context of gene expression with respect to space and time. (frontiersin.org)
  • Therefore, establishing the pathways regulating these patterns could allow linking gene activities with specific outcomes in behavior. (pnas.org)
  • Many oncogenic signalling pathways can induce EMT, but the specific mechanisms of how this occurs, and how this process is controlled are not fully understood. (soton.ac.uk)
  • RESULTS: RNA-Seq analysis of colorectal cancer cells expressing mutant and wild-type β-catenin and analysis of colorectal cancer cells expressing inducible mutant RAS showed that ELF3 expression is reduced in response to oncogenic signalling and antagonizes Wnt and RAS oncogenic signalling pathways. (soton.ac.uk)
  • maturation
  • Interestingly, this mutation accelerated arterial maturation, as observed in retina vessel morphology and aberrant α-smooth muscle actin localization, particularly in vascular pericytes. (jci.org)
  • mutations
  • Several mutations that affect the early stages of the morphogenesis phase correspond to genes involved in morphogenetic processes that occur during vegetative development. (plantphysiol.org)
  • Mutations in the X-linked MID1 gene are responsible for Opitz G/BBB syndrome, a malformation disorder of developing midline structures. (springer.com)
  • Mutations in the X-linked MID1 gene cause Opitz G/BBB syndrome (OS). (springer.com)
  • Von Hippel-Lindau (VHL) gene mutations induce neural tissue hemangioblastomas, as well as highly vascularized clear cell renal cell carcinomas (ccRCCs). (jci.org)
  • We show Vhl mutations also disrupt Notch signaling, causing mutation-specific vascular abnormalities, e.g., type 1 (null) vs. type 2B (murine G518A representing human R167Q). (jci.org)
  • atml1 pdf2 double mutation results in severe defects in shoot epidermal cell differentiation, which are not observed in plants carrying mutations of only one of the genes. (biologists.org)
  • Although biochemical assays exist for some viral RNA polymerases that permit quantitative measure of incorporation fidelity, here we describe a simple method of measuring mutation frequencies of RNA viruses that has proven to be as accurate as biochemical approaches in identifying fidelity altering mutations. (jove.com)
  • LNA-ON-mediated targeted mutagenesis has been used to introduce point mutations into genes of interest in cultured cells of both yeast and mice 3,4 . (jove.com)
  • It is caused by deletions or mutations of the survival of motor neuron 1 gene (SMN1). (jove.com)
  • The starting constructs are GFP negative as the GFP gene is inactivated by an additional exon, or by mutations. (jove.com)
  • genome
  • However, long noncoding RNAs (lncRNAs) represent the largest fraction of transcriptional output in the mammalian genome. (biomedcentral.com)
  • By generating an H5-expressing cell line, we were able to isolate a deletion virus that lacks the H5 gene and show definitively that genome synthesis does not occur in the absence of H5. (embl.de)
  • Given the ever expanding number of model plant species for which complete genome sequences are available and the abundance of bio-resources such as knockout mutants, wild accessions and advanced breeding populations, there is a rising burden for gene functional annotation. (jove.com)
  • Within the relatively large (~ 200 kb) vaccinia genome, three classes of genes are encoded: early, intermediate, and late. (jove.com)
  • While transcriptomic and proteomic approaches have been the methods of choice to study genome-wide changes in neuronal gene expression, the "when and where" aspects are lost during biochemical extraction, providing only ensemble measurements. (frontiersin.org)
  • Analysis of gene-expression patterns across The Cancer Genome Atlas (TCGA) and protein localization in colorectal cancer tumour panels showed that ELF3 expression is anti-correlated with β-catenin and markers of EMT and correlates with better clinical prognosis. (soton.ac.uk)
  • sequence
  • RNA-binding protein that binds to 5'ACACCC-3' core sequence, termed zipcode, within the 3'UTR of ITGA3. (uniprot.org)
  • Sequence analysis revealed that most of SlHsp20 genes possessed no intron or a relatively short intron in length. (frontiersin.org)
  • ATSs in which an exogenous gene sequence is positioned 5' to the viral structural genes is used for stable protein expression in insects. (jove.com)
  • ATSs, in which a gene sequence is positioned 3' to the structural genes, is used to trigger RNAi and silence expression of that gene in the insect. (jove.com)
  • Although determination of the sequence of every gene in Volvox or any other species allows a better understanding of the organism's physiological potential, it is just the first step of a complete description of how the organism works. (biomedcentral.com)
  • introns, 5′ and 3′ UTRs in grey) has cis-acting localization elements in its primary sequence. (nih.gov)
  • Nucleotide anddeduced amino acid sequence of XLPOUl.Positionsofthe bases areshowntotherightofthe nucleotidesequence.The POU-specific domain and homeodomain are underlined. (xenbase.org)
  • polymerases
  • While all three classes are transcribed by virally-encoded RNA polymerases, each class serves a different function in the life cycle of the virus. (jove.com)
  • functional
  • Two SlHsp20 genes displayed differential expression levels between cultivated tomato and S. pimpinellifolium in vegetative (leaf and root) and reproductive organs (floral bud and flower), suggesting inter-species diversification for functional specialization during the process of domestication. (frontiersin.org)
  • However, testing specific features of the visual system provide more empirical evidence for functional applications. (jove.com)
  • These interactions are mediated by specific functional groups within the RNA. (searlescholars.net)
  • In this Research Topic, we aim to bring together original research and review articles that elucidate the causality between gene expression and functional/structural plasticity of neurons in health and disease using imaging, and that also encompass new imaging methods and photo-manipulation techniques to investigate neuronal gene expression. (frontiersin.org)
  • This review examines evidence showing that genes and gene networks important for myelination can be regulated by functional activity in axons. (frontiersin.org)
  • Successful repair of the I-SceI-induced breaks by NHEJ or HR restores the functional GFP gene. (jove.com)
  • mutation
  • Increasing evidence shows that the intrinsic error rates, and the resulting mutation frequencies, of RNA viruses can be modulated by subtle amino acid changes to the viral polymerase. (jove.com)
  • The remaining copy of the gene, SMN2, generates only low levels of the SMN protein due to a mutation in SMN2 exon 7 that leads to exon skipping. (jove.com)
  • In a screen for modifiers of the oocyte DV patterning defects, we identified a mutation in the eIF1A gene as a dominant suppressor. (biologists.org)
  • processes
  • This is particularly important in establishing how local gene expression is essential for processes like axon guidance, network formation and synaptic plasticity. (frontiersin.org)
  • To identify the molecular processes leading to these adaptations, we performed Cre/loxP-mediated genetic ablations of two key regulators of gene expression in response to activity, the Ca 2+ /calmodulin-dependent protein kinase IV (CaMKIV) and its postulated main target, the cAMP-responsive element binding protein (CREB). (pnas.org)
  • embryonic development
  • MID1 expression is tightly regulated through concerted action of alternative promoters and alternative polyadenylation signals both during embryonic development and in the adult. (springer.com)
  • Christiansen J. H., Dennis C. L., Wicking C. A., Monkley S. J., Wilkinson D. G., and Wainwright B. J. (1995) Murine Wnt-11 and Wnt-12 have temporally and spatially restricted expression patterns during embryonic development. (springer.com)
  • sequences
  • Precursor messenger RNA (pre-mRNA) splicing is an essential step in gene expression mediated by the spliceosome, a large protein complex in the cell nucleus that interacts with specific intronic sequences in the pre-mRNA called splice sites for the proper removal of introns and correct joining of exons. (mdpi.com)
  • viral
  • For a more precise investigation of how H5 contributes to DNA synthesis, we placed the ts57 H5 allele in an otherwise wild-type viral background and also performed small interfering RNA-mediated depletion of H5. (embl.de)
  • Foreign gene expression is made possible by the insertion of an additional viral subgenomic RNA initiation site or promoter. (jove.com)
  • In particular, the expression of fluorescent and bioluminescent reporters has been instrumental tracking the viral infection in the vector and virus transmission 5,14-16,18 . (jove.com)
  • This technique has been used for delivery of adeno-associated virus (AAV) vectors, which in turn can provide almost body-wide, stable transgene expression for the life of the animal depending on the viral serotype chosen. (jove.com)
  • proteomic
  • For diagnostic applications, monitoring (detecting) the presence, absence, or abnormal expression of targeted proteomic or genomic biomarkers found in patient samples can be used to determine treatment approaches or therapy efficacy. (jove.com)
  • annotation
  • In this protocol, annotation of plant gene function using combined co-expression gene analysis, metabolomics and informatics is provided ( Figure 1 ). (jove.com)
  • B ) Summary of relevant features of the MiMIC insertion collection based on the FlyBase 6.01 gene annotation. (nih.gov)
  • Note that the counts of insertions associated with features of the gene annotation do not sum to the total number of insertions. (nih.gov)
  • nascent
  • Before this information can be relayed to the cytoplasm, the nascent RNA undergoes extensive post-transcriptional processing before arriving at its final destination in cells. (mdpi.com)
  • binds
  • Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs. (uniprot.org)
  • We show here that PROTODERMAL FACTOR2 ( PDF2 ), a member of the HD-GL2 class of homeobox genes, is expressed exclusively in the L1 of shoot meristems and that recombinant PDF2 protein specifically binds to the L1 box in vitro. (biologists.org)
  • The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. (merckmillipore.com)
  • Moreover
  • Moreover, combined WM-FISH/-FIHC approaches using an AgOR2-specific riboprobe and AgOBP-specific antisera revealed the expression of the "ligand-matched" AgOBP1, AgOBP4 and AgOR2 to single trichoid hairs. (jove.com)