• We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting from treatment of hepatocytes with cytokines and hormones known to influence synthesis of other proteins of hepatic origin. (nih.gov)
  • Thus the inhibitory effects of SLTM on gene expression appear to result from generalized down-regulation of mRNA synthesis and initiation of apoptosis consequent upon overexpressing the protein. (bris.ac.uk)
  • RNA codons and protein synthesis. (crossref.org)
  • Maintenance of fat free mass is dependent upon nutrient stimulation of protein synthesis via the mTOR complex, although during caloric restriction a decrease (atrophy) in skeletal muscle may be driven by a homeostatic shift favouring protein catabolism. (biomedcentral.com)
  • Emerging evidence suggests a high ratio of protein to carbohydrate in a low-fat, caloric restricted (CR) diet may mitigate FFM reductions during weight loss through increasing muscle protein synthesis and/or reducing protein catabolism, thereby improving net muscle protein balance[ 6 ]. (biomedcentral.com)
  • Herpes simplex virus type 1 (HSV-1) transcription can be arrested at the immediate early (IE) stage by continuous treatment of cells with inhibitors of protein synthesis, usually cycloheximide, from the time of infection. (microbiologyresearch.org)
  • Expression from the HSV-1 IE promoters that control synthesis of ICP0 and ICP27 was, unexpectedly, stimulated by inhibition of protein synthesis. (microbiologyresearch.org)
  • Expression from the human cytomegalovirus major IE promoter, when cloned into the genome of HSV-1 mutants, was also increased by inhibition of protein synthesis. (microbiologyresearch.org)
  • Activation of the ICP0 promoter was observed when protein synthesis was blocked by alternative agents. (microbiologyresearch.org)
  • Since inhibitors of protein synthesis are known to activate cellular signal transduction pathways, our findings demonstrate new mechanisms for the regulation of HSV-1 IE gene expression which may be important during latency and reactivation. (microbiologyresearch.org)
  • Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate early gene products by protein synthesis inhibition. (microbiologyresearch.org)
  • Protein synthesis inhibitors differentially superinduce c-fos and c-jun by three distinct mechanisms: lack of evidence for labile repressors. (microbiologyresearch.org)
  • Differential dependence of herpes simplex virus immediate-early gene expression on de novo-infected cell protein synthesis. (microbiologyresearch.org)
  • Effect of protein synthesis inhibitors on growth factor activation of c- fos , c- myc , and actin gene transcription. (microbiologyresearch.org)
  • We recently reported that plasma albumin redox state, which correlates with albumin synthesis rate, could be associated with the quality of dietary protein. (frontiersin.org)
  • Plasma albumin redox state was shifted to a more oxidized state in rats fed 3% casein (CN) diet than those fed 3% whey protein or 3% wheat gluten diet, while supplementing 3% CN diet with cystine reversed it to a more reduced state, indicating that cystine would complement the shortage of cysteine in CN, thereby increasing albumin synthesis rate. (frontiersin.org)
  • We have consecutively reported that the redox state of plasma albumin (the balance of reduced and oxidized forms of plasma albumin) is influenced by the amount of protein intake in rats ( 13 - 15 ), and the ratio of MA to total albumin correlates with the albumin fractional synthesis rate, which is also stimulated by dietary proteins ( 14 ). (frontiersin.org)
  • 2022 ) Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae . (lu.se)
  • Finally, FZ-mediated expression of the Arabidopsis transcription factor MYB75 caused N. benthamiana to bear brown leaves and purple, twisted flowers, indicating that MYB75 retained the function of activating anthocyanin synthesis pathways in a different plant. (researchsquare.com)
  • With gene synthesis, scientists are able to create artificial genes in a laboratory setting for further study. (synbio-tech.com)
  • This is where Synbio Technologies comes in, offering a gene synthesis protein expression package that ensures the necessary amount of protein expression critical to various fields in the genetics industry. (synbio-tech.com)
  • That's why our gene synthesis protein expression package offers up to 5 mg with 85% purity of the requested protein, guaranteed to be delivered within a six-week timeframe. (synbio-tech.com)
  • Our unique and revolutionary Syno Gene Synthesis Platform allows us to generate any requested genetic sequence up to and including 150 Kb in length, all while optimizing the gene synthesis protein expression along the way. (synbio-tech.com)
  • What sets us apart from the "do it yourself" process is our top-notch codon optimization, gene synthesis, protein expression, and 2-step purification process. (synbio-tech.com)
  • Even more impressive is our pricing, which, on average, is over $1,000 cheaper than the "do it yourself" method, even when gene synthesis with codon optimization is included. (synbio-tech.com)
  • Our gene synthesis protein expression package is the perfect solution to all of our customer's gene synthesis expression needs, ensuring ease and efficiency in studying the proper gene synthesis expression level. (synbio-tech.com)
  • In conclusion, the study of DNA and genes is an ever-evolving field, and at Synbio Technologies, we pride ourselves in offering one of the premier gene synthesis protein expression packages in the industry. (synbio-tech.com)
  • By utilizing our Syno Platform, top-notch processes, and advanced gene synthesis techniques, we strive to provide our customers with the highest quality products at an affordable price. (synbio-tech.com)
  • Our goal is to make gene synthesis as accessible as possible, and we are committed to providing our customers with the best experience possible. (synbio-tech.com)
  • Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tuberization in vitro [J]. J Integr Plant Biol. (jipb.net)
  • This is a short video showing how to improve protein expression yields with GeneArt Gene Synthesis and Gateway expression vectors. (thermofisher.com)
  • For example, viral infection, stress and the regulation of other proteins can also change the aggregation of p53 protein ( 20 ). (spandidos-publications.com)
  • In our proposed project, you would investigate expression regulation in clinically significant bacterium non-typeable Haemophilus influenzae (NTHi). (lu.se)
  • 2022 ) Gene Expression Regulation in Airway Pathogens: Importance for Otitis Media. (lu.se)
  • However, we did not observe any regulation of the OsDREB1B gene expression by light under control conditions. (unl.pt)
  • Researchers have identified a host of factors involved in epigenetic control of gene expression and development, but many of the precise mechanisms underlying this type of regulation remain unclear. (mdanderson.org)
  • Hha, along with the chromatin-associated protein H-NS, is involved in the regulation of expression of the toxin alpha-haemolysin in response to osmolarity and temperature. (wikipedia.org)
  • Investigation of UTR Variants by Computational Approaches Reveal Their Functional Significance in PRKCI Gene Regulation. (nih.gov)
  • Developmental regulation of neuronal gene expression by Elongator complex protein 1 dosage. (figshare.com)
  • RafK carries a C-terminal regulatory domain present in a subset of ATP-binding proteins that has been involved in both direct regulation of transporter activity (inducer exclusion) and transcription of transporter genes. (lu.se)
  • S100A8 mRNA and S100A8 protein levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. (medscimonit.com)
  • Although SLTM also suppressed the ability of oestrogen to activate a reporter gene in MCF-7 breast-cancer cells, inhibition of a constitutively active beta-galactosidase gene suggested that this was primarily the consequence of a generalized inhibitory effect on transcription. (bris.ac.uk)
  • Transcription factors interacting with herpes simplex virus a gene promoters in sensory neurons. (microbiologyresearch.org)
  • They found that TDRD3 forms a complex with a protein called topoisomerase 3B (TOP3B), an enzyme that unwinds DNA and, in so doing, prevents DNA structures from forming that impede gene transcription. (mdanderson.org)
  • R-loop accumulation at a gene can prevent transcription, and these loops are also often sites of DNA breaks. (mdanderson.org)
  • Together, transcription and translation are known as gene expression. (medlineplus.gov)
  • Through the processes of transcription and translation, information from genes is used to make proteins. (medlineplus.gov)
  • Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold. (nih.gov)
  • In a model with a changing volume that follows the cell cycle, we integrate successively the protein production mechanism (transcription and translation), the random segregation of compounds at divi- sion, and the gene replication. (hal.science)
  • Conservation of order and transcription orientation of two proximal genes between fishes and mammals strongly indicates gene orthology. (nih.gov)
  • Tumor protein 53 ( TP53 ) is a recognized tumor suppressor gene located on chromosome 17q13.1. (spandidos-publications.com)
  • Gene and chromosome 7 copy numbers were identified by fluorescent in situ hybridization (FISH). (nih.gov)
  • The prevalent FISH patterns were balanced disomy (40%) and trisomy (38%) for EGFR gene and chromosome 7 (40%), whereas balanced polysomy was seen in 13% and gene amplification was seen in 9% of the patients. (nih.gov)
  • This gene lies within the major histocompatibility complex class I region on chromosome 6, and alternatively spliced transcript variants have been observed for this gene. (nih.gov)
  • A chromosome contains a single, long piece of DNA with many different genes. (cdc.gov)
  • The pump mutation is in ATP2C1 , a gene localized on chromosome 3. (medscape.com)
  • We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)
  • Utilizing our previous interactome perturbation predictor, we identify a set of missense variants that are likely disruptive to protein-protein interactions. (springer.com)
  • For genes encoding the disrupted interactions, we evaluate their expression patterns across developing brains and within specific cell types, using both bulk and inferred cell-type-specific brain transcriptomes. (springer.com)
  • Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
  • Extending earlier work, we show that de novo missense variants that disrupt protein interactions are enriched in individuals with ASD, often affecting hub proteins and disrupting hub interactions. (springer.com)
  • Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
  • Disrupted protein interactions identify gene sets involved in risk for ASD. (springer.com)
  • Background: Large-scale identification of the interrelationships between different components of the cell, such as the interactions between proteins, has recently gained great interest. (ugent.be)
  • Results: In this study, we have developed a computational method that exploits the existing knowledge on protein-protein interactions in diverse species through orthologous relations on the one hand, and functional association data on the other hand to predict and filter protein-protein interactions in Arabidopsis thaliana. (ugent.be)
  • A highly reliable set of protein-protein interactions is predicted through this integrative approach making use of existing protein-protein interaction data from yeast, human, C. elegans and D. melanogaster. (ugent.be)
  • Localization, biological process, and co-expression data are used as powerful indicators for protein-protein interactions. (ugent.be)
  • The functional repertoire of the identified interactome reveals interactions between proteins functioning in well-conserved as well as plant-specific biological processes. (ugent.be)
  • Conclusion: We conclude that the integration of orthology with functional association data is adequate to predict protein-protein interactions. (ugent.be)
  • Through this approach, a high number of novel protein-protein interactions with diverse biological roles is discovered. (ugent.be)
  • Overall, we have predicted a reliable set of protein-protein interactions suitable for further computational as well as experimental analyses. (ugent.be)
  • De Bodt S, Proost S, Vandepoele K, Rouzé P, Van de Peer Y. Predicting protein-protein interactions in Arabidopsis thaliana through integration of orthology, gene ontology and co-expression. (ugent.be)
  • The authors set out to find clues to TDRD3's function by looking for physical interactions between TDRD3 and other proteins in the cell. (mdanderson.org)
  • The last model integrates then all the genes of the cell and takes into account their interactions in the productions of different proteins through a common sharing of RNA-polymerases and ribosomes, available in limited quantities. (hal.science)
  • The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)
  • DNA is tightly packaged into chromosomes along with histone proteins. (mdanderson.org)
  • The BCG vaccine has been shown to increase methylation of one of the histone proteins, resulting in increased cytokine production in certain immune cells. (cdc.gov)
  • The type of RNA that contains the information for making a protein is called messenger RNA (mRNA) because it carries the information, or message, from the DNA out of the nucleus into the cytoplasm. (medlineplus.gov)
  • In addition, chromobox proteins CBX5/HP1α and CBX3/HP1γ cooperated with the PEX domain in induction of HSP70B′ mRNA. (elsevierpure.com)
  • The aim of this study was to investigate the expression and silencing of the S100A8 gene, which encodes the S100 calcium-binding protein A8 (S100A8), and apoptosis and phosphorylation of protein kinase B (Akt) in tissue samples of endometrial carcinoma and HEC-1A endometrial adenocarcinoma cells in vitro . (medscimonit.com)
  • This gene encodes a protein phosphatase 1 binding protein. (nih.gov)
  • This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. (nih.gov)
  • The gene ATP2C1 encodes the human secretory pathway Ca ++ -ATPase hSPCA1, which is dysfunctional and causes abnormal calcium release from the Golgi apparatus and endoplasmic reticulum. (medscape.com)
  • The BCG bacterium has lost a comparatively large quantity of genomic DNA through attenuation since its primary production in 1921, of which the majority represented genes encoding antigenic proteins. (herts.ac.uk)
  • Therefore, the overexpression of p53 does not always indicate a mutation in the TP53 gene. (spandidos-publications.com)
  • 001). EGFR overexpression or high gene copy numbers had no significant influence on prognosis. (nih.gov)
  • EGFR overexpression is frequent in NSCLC, is most prominent in SCC, and correlates with increased gene copy number per cell. (nih.gov)
  • Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. (researchsquare.com)
  • An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. (researchsquare.com)
  • However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption. (researchsquare.com)
  • Comprehensive recombinant protein expression and purification services. (synbio-tech.com)
  • The drug substance anakinra is a recombinant protein (Interleukin-1 receptor antagonist) produced in E. coli bacteria containing an expression plasmid in which a synthetic gene coding for human IL-1Ra has been inserted.According to the Guideline on the Environmental Risk Assessment of Medicinal Products for Human Use (EMEA 2006), proteins are exempted from environmental risk assessment because they are unlikely to result in significant risk to the environment. (janusinfo.se)
  • and b) liberate bioactive peptides that are able to exhibit physiological responses that otherwise would be diminished from consuming intact protein sources. (biomedcentral.com)
  • The use of amino acids, proteins and peptides is not considered to have any environmental impact. (janusinfo.se)
  • According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CHMP/SWP/4447/00),vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids proteins, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)
  • We select from GenBank a group of 138 sequences and obtained a consensus PDCoV membrane protein (M-PDCoV) sequence of 216 a.a. (authorea.com)
  • Over the past few decades it has become increasingly clear that epigenetic changes - heritable changes in the cell that do not result from DNA sequence alteration - may be just as important as gene mutations in cancer development. (mdanderson.org)
  • We then put the sequence through expression tests to optimize the synthesized gene's expression levels, ensuring that it meets the customer's request. (synbio-tech.com)
  • Protein assembly continues until the ribosome encounters a "stop" codon (a sequence of three nucleotides that does not code for an amino acid). (medlineplus.gov)
  • Potato virus Y (PVY) N coat protein (CP) coding sequence was cloned into a plant expression vector pMON316 under the CaMV 35S promoter. (jipb.net)
  • Cloning and Sequencing of Coat Protein Gene of Papaya Ringspot Virus and Sequence Comparison of Strains of PRV-P and PRV-YS [J]. J Integr Plant Biol. (jipb.net)
  • Amplification of Soybean Mosaic Virus Coat Protein Gene by Polymerase Chain Reaction and Its Sequence Analysis [J]. J Integr Plant Biol. (jipb.net)
  • The cDNA Cloning and Nucleotide Sequence of Potato Virus X Coat Protein Gene [J]. J Integr Plant Biol. (jipb.net)
  • We report evidence from cDNA isolation and expression analysis as well as analyses of genome, expressed sequence tag (EST), cDNA and expression databases for a new gene named SPRN (shadow of prion protein). (nih.gov)
  • The specific order, or sequence, of these bases determines the exact information carried in each gene (for example, instructions for making a specific protein). (cdc.gov)
  • We show here that in vitro exposure to SARS-CoV-2 activates the expression of the human endogenous retrovirus (HERV) HERV-W proinflammatory envelope protein (ENV) in peripheral blood mononuclear cells from a subset of healthy donors, in ACE2 receptor and infection-independent manner. (unmc.edu)
  • This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
  • Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
  • Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)
  • bHLH proteins are able to bind to hexameric E-box (CANNTG) or N-box (CACG(A/C)G) motifs, depending on transcriptional activity. (unl.pt)
  • Several types of chemical modifications of the five major histone protein families give rise to a complex 'histone code' that signals the transcriptional machinery to turn genes on or off. (mdanderson.org)
  • however, further transcriptional target genes and cofactors of intranuclear MMP3 have not been uncovered. (elsevierpure.com)
  • In this paper, we used transcriptomics analysis in order to examine novel transcriptional target genes regulated by intracellular MMP3. (elsevierpure.com)
  • The hemopexin-like repeat (PEX) domain of the human MMP3 was essential for transcriptional induction of the HSP70B′ gene. (elsevierpure.com)
  • Taken together, this study newly clarified that intracellular MMP3 cooperate with CBXs/HP1s in transcriptional promotion of HSP genes. (elsevierpure.com)
  • Alternatively nuclear receptor coactivators can function as adaptor proteins that bring nuclear receptors into close proximity with transcriptional complexes. (bvsalud.org)
  • Further, gene length analysis of the differentially expressed genes showed that the loss of Elp1 mainly impacts the expression of long genes and that by gradually restoring Elongator their expression is progressively rescued. (figshare.com)
  • Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
  • Fig. 7: ASAP-seq enables detection of intracellular proteins with barcoded antibodies. (nature.com)
  • A new study by Mark Bedford, Ph.D. , professor of Molecular Carcinogenesis , and colleagues published in Molecular Cell sheds light on the mechanism by which TDRD3 - a protein that 'reads' epigenetic marks on chromosomal proteins - turns on certain genes. (mdanderson.org)
  • Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were performed to detect the expression of p53 protein and gene deletion in ESCC tissue samples from different ethnic groups in Xinjiang, in order to analyze their association with clinicopathological characteristics and patient prognosis, as well as the sensitivity and specificity of the two methods. (spandidos-publications.com)
  • The detection of mutant p53 protein by immunohistochemistry (IHC) has some limitations. (spandidos-publications.com)
  • Immunohistochemistry (IHC) was used to detect expression of the S100A8 protein in 74 tissue samples of endometrial cancer and 22 normal endometrial tissue samples. (medscimonit.com)
  • Protein expression was assessed by immunohistochemistry on a scale from 0 to 400 (percentage of positive cells x staining intensity). (nih.gov)
  • Paired CRC tumorous and ANNT were examined for HCMV UL55 by PCR and for IE/ E proteins by immunohistochemistry (IHC). (bvsalud.org)
  • As of August 2017, Expression Atlas holds data from 3,126 studies across 33 different species, including 731 from plants. (oregonstate.edu)
  • De Iaco also found that when DUX4 is forcibly produced in muscle cells, it turns on a whole set of genes that are expressed during zygotic genome activation. (news-medical.net)
  • They found that DUX4 is one of the very first genes expressed at this stage, releasing a high concentration of its protein product just before zygotic genome activation. (news-medical.net)
  • In line with this lead, the scientists could show that the DUX4 protein binds to the regulatory region of genes that are induced during zygotic genome activation, stimulating their expression. (news-medical.net)
  • The final piece of evidence came when the EPFL scientists removed the DUX gene from fertilized mouse oocytes using CRISPR/Cas9 genome editing. (news-medical.net)
  • The study points to DUX4, and by extension the DUX family of proteins, as the master regulator responsible for kick-starting genome expression at the earliest stage of embryonic life in humans, mouse and probably all placental mammals. (news-medical.net)
  • Whole-genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation. (ethz.ch)
  • The gradual conversion from endosymbiont to organelle during the course of evolution has clearly been accompanied by a dramatic reduction in genome size as the chloroplasts lost most of their genes to the nucleus. (frontiersin.org)
  • Southern blot indicated that the PCR product was indeed PVY CP gene which had been integrated into the potato genome. (jipb.net)
  • Could gene editing give humans the ability to regrow lost limbs? (thetech.org)
  • Specifically we evaluate the effect of increased dietary protein intake and caloric restricted diets on gene expression in skeletal muscle, particularly focusing on biosynthesis, degradation and the expression of genes in the ubiquitin-proteosome (UPP) and mTOR signaling pathways, including MuRF-1, MAFbx/atrogin-1, mTORC1, and S6K1. (biomedcentral.com)
  • We then conducted a comprehensive transcriptome analysis in mouse embryos to identify genes and pathways whose expression correlates with the amount of ELP1. (figshare.com)
  • A Transgenic Study on Affecting Potato Tuber Yield by Expressing the Rice Sucrose Transporter Genes OsSUT5Z and OsSUT2M [J]. J Integr Plant Biol. (jipb.net)
  • Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)
  • Genes (Basel), 2023 Jan 18. (nih.gov)
  • In Expression Atlas, users can query genes or gene-sets of interest and explore their expression across or within species, tissues, developmental stages in a constitutive or differential context, representing the effects of diseases, conditions or experimental interventions. (oregonstate.edu)
  • Treatment with VPA and LiCl resulted in the differential expression of 331 and 164 genes respectively. (plos.org)
  • There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)
  • The flow of information from DNA to RNA to proteins is one of the fundamental principles of molecular biology. (medlineplus.gov)
  • Genes encoding disrupted complementary interactors tend to be risk genes, and an interaction network built from these proteins is enriched for ASD proteins. (springer.com)
  • However, unraveling large-scale protein-protein interaction maps is laborious and expensive. (ugent.be)
  • The clustering algorithms used to analyze the information contained in protein-protein interaction network are effective ways to explore the characteristics of protein functional modules. (biomedcentral.com)
  • We have shown that OsPIF14 binds to the OsDREB1B promoter through two N-boxes and that the flanking regions of the hexameric core are essential for protein-DNA interaction and stability. (unl.pt)
  • When the interaction propensity score of a disordered region exceeds that of the full-length protein (points above the red line), disorder is considered to promote interaction with RNA molecules. (biomedcentral.com)
  • (B) For 66% of the proteins (137 entries), disorder contributes at low interaction propensities, while full-length protein sequences dominate at high interaction propensities (Mann-Whitney U test). (biomedcentral.com)
  • Interaction propensities are averaged per protein. (biomedcentral.com)
  • More recent data has focused on the potential health benefits of hydrolyzed dietary proteins. (biomedcentral.com)
  • Plasma albumin would thus be influenced by amino acid balance in dietary proteins, and it could be useful as a biomarker that contributes to prevention of protein under-nutriton, caused by not only insufficient protein intake but also ingestion of poor-quality protein. (frontiersin.org)
  • Calculating the similarity between gene expression patterns (co-expression degree) by using gene expression data has an important guiding function in understanding the relationship between the corresponding proteins of the gene, and can help to identify whether different proteins have same or similar functions and whether they can be composed as protein complexes or functional modules. (biomedcentral.com)
  • abstract = "DREB1/ CBF genes, known as major regulators of plant stress responses, are rapidly and transiently induced by low temperatures. (unl.pt)
  • Trained immunity is mediated through epigenetics , the modification of gene expression without changing the genetic code. (cdc.gov)
  • Gene ontology (GO) term enrichment analysis was used to highlight the most relevant GO terms associated with a given gene list following toxin exposure. (plos.org)
  • Altogether, these results demonstrate that SARS-CoV-2 could induce HERV-W envelope protein expression and suggest its involvement in the immunopathogenesis of certain COVID-19-associated syndromes and thereby its relevance in the development of personalized treatment of patients. (unmc.edu)
  • When Gm SubPep is supplied to soybean cultures, it was found to induce the expression of defense genes. (isaaa.org)
  • The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • When in culture, a small fraction of these cells exhibit at any given time the gene expression pattern of 2-cell stage embryos, before cycling back to the features of more advanced embryonic cells. (news-medical.net)
  • I just read about scientists using CRISPR to fix a gene in human embryos. (thetech.org)
  • Time Course Study: Knowledge of time dependent cytokine expression following M. bovis infection would aid vaccine development by revealing potential correlates of protection. (herts.ac.uk)
  • Further analysis of the data as a profile of cytokine expression for each lymph node type revealed that IFN-γ was dominantly expressed within the LB and CRM nodes, whereas within the CM and CRT nodes, IL10 was the dominant cytokine. (herts.ac.uk)
  • Rice phytochrome-interacting factor protein OsPIF14 represses OsDREB1B gene expression through an extended N-box and interacts preferentially with the active form of phytochrome B . Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms , 1859 (2), 393-404. (unl.pt)
  • A few genes produce regulatory molecules that help the cell assemble proteins. (medlineplus.gov)
  • Like RafK, the presence of DLDH also activated the expression of raf operon genes, as DLDH-negative pneumococci showed a significantly decreased expression of aga and rafEFG, but DLDH did not regulate rafK or the putative regulatory genes rafR and rafS. (lu.se)
  • The study of protein complexes and protein functional modules has become an important method to further understand the mechanism and organization of life activities. (biomedcentral.com)
  • This paper conducts an intensive study on the problems of low recognition efficiency and noise in the overlapping structure of protein functional modules, based on topological characteristics of PPI network. (biomedcentral.com)
  • The experimental results show that the ECTG algorithm can detect protein functional modules better. (biomedcentral.com)
  • We find that topological structure and attribute information are very effective in identifying protein complexes by analyzing the existing mainstream PPI network methods for identifying protein functional modules [ 12 , 13 ], even though there are not much approaches take both information into consideration. (biomedcentral.com)
  • We have analysed the effect of cycloheximide on IE gene expression with HSV-1 mutants deficient in the production of functional levels of the three major transactivators, the virion protein (VP16) and two IE proteins (ICP0 and ICP4). (microbiologyresearch.org)
  • The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
  • Virus-induced gene silencing (VIGS) is an alternative functional genomics tool for soybean because it does not involve the generation of inheritable changes. (researchsquare.com)
  • Most genes contain the information needed to make functional molecules called proteins. (medlineplus.gov)
  • This result together with some common structural features led to the suggestive hypothesis of a possible functional link between mammalian PrP and Sho proteins. (nih.gov)
  • Fluorescence in situ hybridization (FISH) is a molecular pathological method with high sensitivity and specificity, which uses specific DNA probes to detect chromosomal aberrations, as well as gene deletion and amplification ( 21 , 22 ). (spandidos-publications.com)
  • The study establishes a link between TDRD3 and an enzyme that unwinds DNA at regions of active gene expression, and provides evidence that this partnership can prevent DNA breakage and chromosomal translocations - two of the hallmarks of cancer. (mdanderson.org)
  • The research team observed that in mice lacking the TDRD3 gene there was an increase in both R-loop formation and chromosomal translocations. (mdanderson.org)
  • This family consists of haemolysin expression modulating protein (Hha) from Escherichia coli and its enterobacterial homologues, such as YmoA from Yersinia enterocolitica, and RmoA encoded on the R100 plasmid. (wikipedia.org)
  • Novel features and visualizations include the on-the-fly analysis of gene set overlaps and the option to view gene co-expression in experiments investigating constitutive gene expression across tissues or other conditions. (oregonstate.edu)
  • During eucaloric conditions the continual breakdown of protein that occurs in the body's organs and vital tissues is replenished in the post-absorptive state via supply of amino acids derived primarily from the skeletal muscle component of FFM[ 7 ]. (biomedcentral.com)
  • The study aimed at detecting HCMV UL55 gene, immediate early and early (IE/E) proteins in colorectal tumor tissues and adjacent non neoplastic tissues (ANNT). (bvsalud.org)
  • Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
  • But when the EPFL researchers deleted the DUX gene, this process stopped, the appearance of the 2-cell stage-like subpopulation was suppressed. (news-medical.net)
  • ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
  • This data suggests that the INGAP gene is a novel pancreatic gene expressed during islet neogenesis whose protein product is a constituent of Ilotropin and is capable of initiating duct cell proliferation, a prerequisite for islet neogenesis. (jci.org)
  • Using inferred gene co-expression for three neuronal cell types-excitatory, inhibitory, and neural progenitor-we implicate several hundred genes in risk (FDR \(\le \hspace{0.17em}\) 0.05), ~ 60% novel, with characteristics of genuine ASD genes. (springer.com)
  • Across cell types, these genes affect neuronal morphogenesis and neuronal communication, while neural progenitor cells show strong enrichment for development of the limbic system. (springer.com)
  • Their gene expression during brain development and within cell types highlights how they relate to ASD. (springer.com)
  • Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)
  • The aim of the present study was to investigate the association between tumor protein 53 (TP53) gene deletion and protein expression and clinical features in esophageal squamous cell carcinoma (ESCC), and to evaluate the predictive value of these two characteristics in the prognosis of ESCC. (spandidos-publications.com)
  • A stable S100A8 gene knockdown cell line was constructed using lentiviral packing short hairpin RNA (shRNA) transfected into HEC-1A cells. (medscimonit.com)
  • The effects of expression of the S100A8 gene by endometrial cancer cells was investigated by the MTT assay, cell cycle and apoptotic assays, qRT-PCR, and Western blotting. (medscimonit.com)
  • Expression Atlas ( http://www.ebi.ac.uk/gxa ) is an added value database that provides information about gene and protein expression in different species and contexts, such as tissue, developmental stage, disease or cell type. (oregonstate.edu)
  • The gene structure and expression of the non-specific cytotoxic cell receptor protein (NCCRP-1) in Atlantic cod (Gadus morhua L. (nofima.no)
  • The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system. (wikidata.org)
  • Epidermal growth factor receptor in non-small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis. (nih.gov)
  • High gene copy numbers per cell showed a trend toward poor prognosis. (nih.gov)
  • A cell culture system is here proposed opening new integrative studies on biomineralization, improving our knowledge on the role played by non-collagenous proteins in bone. (sorbonne-universite.fr)
  • Chemical modification of these histones is one way that the cell regulates the packing and unpacking of the DNA and its associated proteins (collectively called chromatin), which in turn helps to determine whether a given gene is activated or repressed. (mdanderson.org)
  • The researchers studied the two proteins in breast cancer cell lines, where they discovered that TDRD3 recruits TOP3B to active chromatin (where genes are turned on) that is marked by arginine methylation. (mdanderson.org)
  • Comparison of Hsps expression after radio-frequency field exposure in three human glioma cell lines. (ethz.ch)
  • 2-GHz band CW and W-CDMA modulated radiofrequency fields have no significant effect on cell proliferation and gene expression profile in human cells. (ethz.ch)
  • The journey from gene to protein is complex and tightly controlled within each cell. (medlineplus.gov)
  • In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
  • Yet, it has been suggested in the literature that the variations of these quantities during the cell cycle may participate to protein vari- ability. (hal.science)
  • DNA wraps around proteins called histones such that some genes can be accessed easily by cell proteins that "read" the gene, increasing gene expression, while others are less accessible, decreasing gene expression. (cdc.gov)
  • The lectures will cover the current status of cell-based protein production systems and the theoretical aspects of the methodology. (lu.se)
  • The algorithm can effectively remove the noise data reflected by calculating the topological structure characteristic values in the PPI network through the similarity of gene expression patterns, and also properly use the information hidden in the gene expression data. (biomedcentral.com)
  • an effect which may be explained by an increased expression of adipose tissue lipolysis-related gene expression patterns following chronic supplementation [ 12 ], an increase in protein-induced thermogenesis (reviewed in [ 15 ]), and/or a consistent reduction in food intake given its satiety-stimulatory effects as discussed above. (biomedcentral.com)
  • Finally, through evaluation of co-expression modules, we identified gene sets with unique expression patterns that depended on ELP1 expression. (figshare.com)
  • The structural similarity is particularly interesting given recent reports of three new genes with similarities to PrPs found in Fugu (PrP-like, PrP-461/stPrP-1 and stPrP-2) and other fish, but for which direct evolution to higher vertebrate PrPs is unlikely and for which no other mammalian homologues have been found. (nih.gov)
  • Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. (nature.com)
  • Upon depletion of RBPJ using CRISPR/Cas9, we observed specific upregulation of Notch target gene expression. (mdpi.com)
  • Here we present ATAC with select antigen profiling by sequencing (ASAP-seq), a tool to simultaneously profile accessible chromatin and protein levels. (nature.com)
  • As technology continues to advance in the field of genetics, the study of DNA and genes has become more accessible. (synbio-tech.com)
  • We examined the acute effects of different dietary protein sources (0.19 g, dissolved in 1 ml of water) on skeletal muscle, adipose tissue and hypothalamic satiety-related markers in fasted, male Wistar rats (~250 g). (biomedcentral.com)
  • Higher amounts of whey versus egg protein stimulate the greatest post-treatment anabolic skeletal muscle response, though test proteins with higher amounts of WPH more favorably affected post-treatment markers related to adipose tissue lipolysis. (biomedcentral.com)
  • Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. (jax.org)
  • Aquaporin-9 Protein Is the Primary Route of Hepatocyte Glycerol Uptake for Glycerol Gluconeogenesis in Mice. (lu.se)
  • Using total DNA purified from transgenic plants as templates and two oligonucleotides synthesized from 5' and 3' of the PVY coat protein gene as primers, the authors carried out polymerase chain reaction (PCR) to check the presence of this gene and obtained a 0. (jipb.net)
  • Enzyme-linked immunosorbent assay (ELISA) of our transgenic plants showed that CP gene was expressed in at least some transgenic potato plants. (jipb.net)
  • Transgenic Potato with PVY Coat Protein Gene and Its Small-Scale Field Test [J]. J Integr Plant Biol. (jipb.net)
  • Studies on Transgenic Potato with High Essential Amino Acid Encoding Gene [J]. J Integr Plant Biol. (jipb.net)
  • Construction of Plant Expression Vectors and Identification of Transgenic Potato Plants [J]. J Integr Plant Biol. (jipb.net)
  • Resistance to Potato Virus X Infection in Transgenic Tobacco Plants with Coat Protein Gene of Virus [J]. J Integr Plant Biol. (jipb.net)
  • A type of RNA called transfer RNA (tRNA) assembles the protein, one amino acid at a time. (medlineplus.gov)
  • Cloning, expression, and characterization of potential immunogenic recombinant porcine deltacoronavirus (PDCoV) membrane protein from a synthetic gene. (authorea.com)
  • Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. (microbiologyresearch.org)
  • Construction and characterization of herpes simplex virus type 1 mutants with defined lesions in immediate early gene 1. (microbiologyresearch.org)
  • Variation in the Coat Protein Gene of Papaya ringspot Virus Isolates from Multiple Locations of China [J]. J Integr Plant Biol. (jipb.net)
  • Resistance to Mixed PVY and PLRV Infection in Potato Cultivars Expressing Dual PVY and PLRV Coat Protein Genes [J]. J Integr Plant Biol. (jipb.net)
  • Cloning and Sequencing of the Gene Encoding Coat Protein of Potato Virus Y [J]. J Integr Plant Biol. (jipb.net)
  • Providing there is adequate dietary protein intake, muscle protein gains which occur in the fed state balances the loss of muscle protein which occurs in the post absorptive state allowing day to day skeletal muscle mass to remain relatively constant[ 7 , 10 ]. (biomedcentral.com)
  • The FFM reduction that typically occurs during CR weight loss also implies a negative net skeletal muscle protein balance. (biomedcentral.com)
  • This, combined with the relatively small numbers of seed each soybean plant produces, makes it extremely challenging to generate mutant seed libraries that cover even 50% of genes. (researchsquare.com)
  • Mutant proteins in familial benign pemphigus create a loss of sensitivity to Ca 2+ and Mn 2+ ion binding and transport. (medscape.com)
  • Hailey-Hailey disease, or familial benign pemphigus, is hypothesized to result from a genetic defect in a calcium pump protein. (medscape.com)
  • [ 6 ] This gene defect is similar to the genetic defect in Darier disease , which also is a calcium pump defect, ATP2A2 . (medscape.com)
  • In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
  • In the subset of VPA targeted genes, 114 were downregulated whilst 217 genes were upregulated. (plos.org)
  • In the subset of LiCl targeted genes, 73 were downregulated and 91 were upregulated. (plos.org)
  • The TP53 gene mutation is the most common gene alteration in many tumors, including esophageal cancer. (spandidos-publications.com)
  • Although the biological significance of TP53 gene mutation is well characterized, its clinical significance in esophageal cancer remains controversial, especially as a prognostic biomarker. (spandidos-publications.com)
  • Similarly, TP53 mutation does not always lead to the accumulation of p53 protein in cells. (spandidos-publications.com)
  • Familial dysautonomia (FD), a hereditary sensory and autonomic neuropathy, is caused by a mutation in the Elongator complex protein 1 (ELP1) gene that lead to a tissue-specific reduction of ELP1 protein. (figshare.com)
  • Dominant diseases can be caused by only one copy of a gene with a DNA mutation. (cdc.gov)
  • For recessive diseases, both copies of a gene must have the DNA mutation for a person to have one of these diseases. (cdc.gov)
  • Alberto de Iaco, a postdoc in the lab of Didier Trono at EPFL, drew upon a seemingly irrelevant study of patients suffering from a form of muscular dystrophy where mutations lead to the production in muscle cells of a protein called DUX4, which is normally detected only at the earliest stage of human embryonic development. (news-medical.net)
  • A portion of human INGAP gene was cloned and appears to be highly homologous to the hamster gene. (jci.org)
  • ARPs, actin-related proteins) exist between different lineages, they are active in specific processes such as growth, cancer metastasis and trichome development in yeast, human and Arabidopsis, respectively. (ugent.be)
  • On long term cultures human bone cells seeded on the osteoid-like matrix displayed a clear osteoblast phenotype as revealed by the osteoblast-like morphology, expression of specific protein such as alkaline phosphatase and expression of eight genes classically considered as osteoblast markers, including BGLAP, COL1A1, and BMP2. (sorbonne-universite.fr)
  • The SPRN gene was found in fish (zebrafish, Fugu) and mammals (mouse, rat, human). (nih.gov)
  • Database information indicates expression of SPRN in embryo, brain and retina of mouse and rat, hippocampus of human, and in embryo and retina of zebrafish, and we directly confirmed a strikingly specific expression of the mammalian (human, mouse, rat) transcripts in whole brain. (nih.gov)
  • Given that FD is caused by a reduction, not loss, of ELP1, we generated two new mouse models by introducing different copy numbers of the human FD ELP1 transgene into the Elp1 knockout mouse (Elp1-/-) and observed that human ELP1 expression rescues embryonic development in a dose dependent manner. (figshare.com)
  • Detection of Human cytomegalovirus UL55 Gene and IE/E Protein Expression in Colorectal Cancer Patients in Egypt. (bvsalud.org)
  • SPRN comprises two exons, with the open reading frame (ORF) contained within exon 2, and codes for a protein of 130-150 amino acids named Shadoo (Japanese shadow), predicted to be extracellular and GPI-anchored. (nih.gov)
  • In the nucleus, SLTM has a punctate distribution and it does not co-localize with SR (serine/arginine) proteins. (bris.ac.uk)
  • Our work to generate a phenotypic mouse model for FD headed to the discovery that homozygous deletion of the mouse Elp1 gene leads to embryonic lethality prior to mid-gestation. (figshare.com)