• 2006). "Regulation of the human taurine transporter by oxidative stress in retinal pigment epithelial cells stably transformed to overexpress aldose reductase" (PDF). (wikipedia.org)
  • DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
  • Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
  • Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
  • In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)
  • In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
  • This cell line very closely mimics the layer of epithelial cells that line the inside of the small intestine [ enterocytes ] and to which STEC adheres to create colonies," Moreira Filho explained. (thecattlesite.com)
  • Sääf AM, Halbleib JM, Chen X, Tsan Yuen S, Yi Leung S, Nelson WJ, Brown PO "Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal and Colon Cancer. (openwetware.org)
  • FUT2 encodes an enzyme that produces histo-blood group antigens (HBGA) on the surface of epithelial cells and in mucosal secretions ( 21 , 22 ). (cdc.gov)
  • Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
  • The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. (biomedcentral.com)
  • These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
  • Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
  • Interestingly, IL-8 has been measured in the colon milieu of healthy volunteers using a dialysis bag inserted in the rectum [ 9 ] suggesting that it might be apically secreted by colonic epithelial cells. (biomedcentral.com)
  • Expression of acrB gene, growth at 37 degrees C and invasiveness of populations in Caco-2 intestinal epithelial cells were assessed. (birmingham.ac.uk)
  • infection with a Gram-positive bacterium leads to altered expression of microRNAs involved in the host cell response in epithelial cells. (uni-giessen.de)
  • Jiang R, Lonnerdal B. Transcriptomic profiling of intestinal epithelial cells in response to human, bovine and commercial bovine lactoferrins. (ucdavis.edu)
  • Chronic exposure of intestinal epithelial cells to IFN-γresults in selective downregulation of NHE2 and NHE3 expression and activity, a potential cause of inflammation-associated diarrhea. (elsevierpure.com)
  • Immune Response Signatures of Benzo(α)pyrene Exposure in Normal Human Mammary Epithelial Cells in the Absence or Presence of Chlorophyllin. (boysandgirlsclubofcypress.com)
  • CYP1A1 and CYP1B1 gene expression and DNA adduct formation in normal human mammary epithelial cells exposed to benzo[a]pyrene in the absence or presence of chlorophyllin. (boysandgirlsclubofcypress.com)
  • However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
  • In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
  • In this study, we determined the effects of the Kigelia africana, the Mimusops zeyheri and the Ximenia caffra seed-oil on cell proliferation in culture. (iiarjournals.org)
  • The suppression of Caco-2 and HEK-293 cell proliferation by K. africana, M. zeyheri and X. caffra seed oils suggest a potential antiproliferative effect of these tree seed oils on the two cell lines. (iiarjournals.org)
  • The objective of this study was to determine the effect of K. africana, M. zeyheri and X. caffra seed oils on Caco-2 and HEK-293 cell proliferation. (iiarjournals.org)
  • APJ‑suppressed fibroblasts treated with an antagonist or small interfering RNA showed myofibroblast‑like properties, including increased proliferation and migratory abilities, via accelerated phosphorylation of Sma‑ and Mad‑related protein 2/3 (Smad2/3). (spandidos-publications.com)
  • This gene-expression profile translated to a phenotype of halted proliferation and included the induction of alkaline phosphatase activity, a marker of epithelial cell differentiation. (monash.edu)
  • SPRY2 knockdown in Caco-2 cells carrying the wild-type (WT) KRAS gene upregulated phosphorylated ERK (p-ERK) levels and increased cell proliferation in vitro, but inhibited cell invasion. (ncku.edu.tw)
  • However, SPRY2 knockdown in SW480 cells (activating KRAS mutant) or Caco-2 cells transfected with KRAS-mutant plasmid did not significantly alter p-ERK levels, cell proliferation, or invasion. (ncku.edu.tw)
  • To further characterize cytochrome P450 (CYP) and P-glycoprotein (Pgp) expression in monolayers of the Caco-2 cell clone TC7, a cell culture model of the human intestinal epithelium. (nih.gov)
  • mRNA expression of eight CYPs and Pgp was investigated in TC7 and parental Caco-2 (Caco-2p) cell monolayers using RT-PCR. (nih.gov)
  • Effects of chlorophyllin on transport ofdibenzo(a, l)pyrene, 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine,and aflatoxin B(1) across Caco-2 cell monolayers. (boysandgirlsclubofcypress.com)
  • The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight junction proteins. (frontiersin.org)
  • Adenine has been suggested to induce cell cycle arrest in cancer cells, leading to cell death [ 9 , 10 ]. (hindawi.com)
  • Although EGF has been demonstrated to induce expression of angiogenic mediators, potential interactions in CRC between EGF-mediated signalling and the hypoxia/HIF pathway remain uncharacterised. (ox.ac.uk)
  • CONCLUSION: These findings suggest that although EGFR phosphorylation activates the MAP kinase signalling and promotes HIF stabilisation in CRC, this alone is not sufficient to induce angiogenic gene expression. (ox.ac.uk)
  • Finally, HIF activation synergises with EGF-mediated signalling to additionally induce a unique sub-group of candidate angiogenic genes. (ox.ac.uk)
  • Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
  • Colonic biopsies from inflamed mucosa of 6 IBD patients (2 with Crohn's disease (CD) and 4 with ulcerative colitis (UC)) were cultured with VPA or control media. (ecco-ibd.eu)
  • Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. (aber.ac.uk)
  • abstract = "Ethnopharmacology relevanceMaytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.Aim of the studyWe investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.Materials and MethodsToxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. (aber.ac.uk)
  • Determining the transepithelial electrical resistance and paracellular permeability of Caco-2 monolayer/macrophage co-cultured system suggested that the OJPs-loaded nanoparticles effectively protected the intestinal epithelial barrier integrity against the damage caused by LPS-stimulated macrophage inflammation and attenuated the defects of intestinal epithelial TJ barrier and permeability. (tmu.edu.tw)
  • Furthermore, permeability was found to be highly temperature-dependent in Chinese hamster ovary (CHO) control cells and artificial membranes (parallel artificial membrane permeability assay). (aspetjournals.org)
  • The results showed that the strain associated with HUS expressed a set of virulence genes that induced a distinct response in human enterocytes, which could help explain its more severe pathogenicity. (thecattlesite.com)
  • Classical virulence genes for this species (tdh, trh) are rarely detected in environmental strains, which are usually considered to lack virulence potential. (umd.edu)
  • Total RNA was extracted and a MiRNA array was performed (miScript™ miRNA PCR Array), profiling the expression of 84 MiRNAs predicted to regulate pro- or anti-inflammatory genes. (ecco-ibd.eu)
  • We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway. (aber.ac.uk)
  • Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge. (aber.ac.uk)
  • With the assistance of CS and WP, the anti-inflammatory and antioxidant activities of OJPs were enhanced because the nanoparticles improved OJPs uptake by RAW264.7 macrophage cells as evidenced by efficient scavenging of DPPH and ABTS free radicals and effective inhibition of NO production and the gene expressions of iNOS, COX2, TNF-α, CCL2, and CXCL2 inflammatory signals. (tmu.edu.tw)
  • The purpose of this study was to determine whether chronic stimulation of intestinal mucosa by interferon-γ (IFN-γ) affects expression and function of the apical membrane Na + /H + exchangers NHE2 and NHE3 in rat intestine and Caco-2/bbe (C2) cells. (elsevierpure.com)
  • The rat HMGCS2 gene contains elements that control its multihormonal regulation and tissue specificity ( 11 - 14 ). (aacrjournals.org)
  • Soen Y, Mori A, Palmer TD, Brown PO "Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. (openwetware.org)
  • In addition, these two binding sites play different roles in the regulation of Sema4D expression in HUVEC and Caco-2 cells. (jcancer.org)
  • Also, siRNA-mediated down-regulation of NFE2L2 did not sensitize SW620 and Caco-2 cells to DHA. (ntnu.no)
  • This coenzyme is essential in energy generation and some other processes including formation of disulfide bonds in proteins, regulation of gene expression and detoxification of reactive oxygen species (ROS) (Groneberg et al. (ijbiotech.com)
  • Down-regulation of MCP-l and MIP-l J3 responses by application of CCR5 deficient mice and MCP-l neutralizing antibody, resulted in minimal effects in inflammatory cell influx but a significant delay in muscle function recovery. (cdc.gov)
  • In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)
  • We therefore undertook a preliminary screening study of the three IFBTS seed oils in vitro using human colon adenocarcinoma cells (Caco-2), a commonly used gastrointestinal cell line, and human embryonic kidney (HEK-293), an epithelial immortalised cell line. (iiarjournals.org)
  • Next, to analyze the response of cells in the inner wall of the small intestine to these two strains, the researchers performed an experiment using the Caco-2 cell line, which is widely used as an in vitro model for studying the human intestinal epithelial barrier. (thecattlesite.com)
  • Halbleib JM, Sääf AM, Brown PO, Nelson WJ "Transcriptional Modulation of Genes Encoding Structural Characteristics of Differentiating Enterocytes During Development of a Polarized Epithelium In Vitro. (openwetware.org)
  • Buess M, Nuyten DS, Hastie T, Nielsen T, Pesich R, Brown PO "Characterization of heterotypic interaction effects in vitro to deconvolute global gene expression profiles in cancer. (openwetware.org)
  • In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
  • From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
  • Altogether, the present in vitro study provides insights into the effects of selected PFASs on B cells, identifying RAG1 and RAG2 expression as possible relevant targets that may play a role in the immunotoxicity of PFASs. (wur.nl)
  • CONCLUSIONS: In vitro exposure to QACFG and triclosan selects for Salmonella Typhimurium cells with reduced susceptibility to several antibiotics. (birmingham.ac.uk)
  • We manipulated SPRY2 gene expression and used an activating KRAS-mutant plasmid to determine its effect on CRC cell function in vitro and/or in vivo. (ncku.edu.tw)
  • Further, using in vitro model, C2C12, a myoblast cell line, we demonstrated that satellite cells express CCR2 and CCRS receptors and these chemokine pathways are involved in satellite cell migration activity. (cdc.gov)
  • Finally, an exploratory, observational, pilot study involving 36 infants diagnosed with at least one FGID (infant colic and/or functional constipation) showed the probiotic formula was well tolerated and FGID severity was significantly reduced after 14 days of treatment with the 2 strains. (frontiersin.org)
  • Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
  • K. africana oil significantly suppressed Caco-2 cell growth compared to HEK-293 cell growth at all oil concentrations, whereas M. zeyheri and X. caffra seed oils significantly suppressed HEK-293 and Caco-2 cell growth, only at a concentration of 80 mg/l. (iiarjournals.org)
  • Luciferase assays showed that T471C and C862T could significantly increase the expression efficiency of downstream target genes. (jcancer.org)
  • APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
  • Crucially, addition of DMOG in combination with EGF significantly increased expression of a further 11 genes (in addition to the 9 genes upregulated in response to either DMOG alone or hypoxia alone). (ox.ac.uk)
  • 4 In this study, we analysed whether VPA influences MiRNA expression in ex-vivo biopsies from IBD patients. (ecco-ibd.eu)
  • VPA increases the expression of MiR449a in cultured ex-vivo biopsies and in Caco-2 cells. (ecco-ibd.eu)
  • Gene expression in intestinal mucosal biopsies revealed different profiles in CD and UC. (nature.com)
  • Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling. (biomedcentral.com)
  • In the present study, we first assessed the effects of PFOA on the transcriptome of the human Namalwa B cell line using RNA seq analysis. (wur.nl)
  • Epithelial barrier function was investigated by transcriptome analysis of fully polarized Caco-2 cells exposed for 6 h to SL or GOS. (monash.edu)
  • Transcriptome analysis showed that SL and GOS both induced pathways that regulate cell cycle control. (monash.edu)
  • We show that expression patterns of particular microRNAs strongly depend on pathogen localization and the presence of bacterial effector proteins. (uni-giessen.de)
  • Stimulation with EGF resulted in EGFR tyrosine autophosphorylation, activation of p42/p44 MAP kinases and stabilisation of HIF-1α and HIF-2α proteins. (ox.ac.uk)
  • Pathogens' inhibition was mostly mediated by the production of organic acids, but neutralization experiments strongly suggested the presence of additional antimicrobial compounds in probiotic culture supernatants such as the bacteriocin Lantibiotic B, whose gene was detected in the genome of B. longum KABP042. (frontiersin.org)
  • Inhibition of cell viability was assessed with focus on apoptosis transcriptionally and translationally. (hindawi.com)
  • 1,2 Modulation of MiRNAs is hypothesized to be a possible mechanism of action of HDAC inhibition. (ecco-ibd.eu)
  • MicroRNA 5703 enriched in exosomes derived from HCT116 sh p53 cells inhibited APJ expression, and inhibition of miR‑5703 diminished APJ suppression in fibroblasts caused by cancer cells. (spandidos-publications.com)
  • Neither vitamin E nor the antioxidants butylated hydoxyanisole (BHA) and butylated hydoxytoluene (BHT) did affect the growth inhibition in SW620 cells after DHA-treatment. (ntnu.no)
  • Inhibition of autophagy by siRNA mediated knock down of ATG5 and ATG7 sensitized both SW620 and Caco-2 cells to DHA. (ntnu.no)
  • Stimulation of autophagy by rapamycin in SW620 and Caco-2 cells resulted in decreased DHA-sensitivity and inhibition of autophagy in Caco-2 cells by chloroquine resulted in increased DHA-sensitivity. (ntnu.no)
  • Semaphorin 4D (Sema4D) has been proven to be one of the hypoxia effectors regulated by hypoxia inducible factor (HIF-1) in multiple cells, and play a role in angiogenesis like VEGF. (jcancer.org)
  • METHODS: PCR-based profiling was applied to identify angiogenic genes in Caco-2 CRC cells regulated by hypoxia, the hypoxia mimetic dimethyloxallylglycine (DMOG) and/or EGF. (ox.ac.uk)
  • qPCR was carried out to determine the changes in mRNA expression of genes of interest. (hindawi.com)
  • We have examined the DHA-induced stress response in two human colon cancer cell lines, SW620 and Caco-2. (ntnu.no)
  • Caco-2 cells, a human colon cancer cell line that can differentiate to a small bowel phenotype, were transiently transfected with human NT/N promoter fragments linked to luciferase and various amounts of Src expression plasmids or dominant negative Raft luciferase and β-galactosidase activities were measured after 48 hours. (uky.edu)
  • Once digested LWE was applied to Caco2 in transport experiments, no significant effect was noticed on the modulation of cholesterol-related gene expression. (nih.gov)
  • Modulation of mitochondrial electron transport chain activity differentially regulates cell death in proliferating and quiescent cells. (mitoeagle.org)
  • Gene expression signature of DMBA-induced hamster buccal pouch carcinomas: modulation by chlorophyllin and ellagic acid. (boysandgirlsclubofcypress.com)
  • Gene expression analysis resulted in 1404 differentially expressed genes upon H(2)O(2) challenge and 979 genes after menadione treatment . (bvsalud.org)
  • Conjugated linoleic acid (CLA) exhibits isomer-specific effects on transepithelial calcium (Ca) transport as well as on cell growth in human intestinal-like Caco-2 cells. (uea.ac.uk)
  • Ablation of DNMT3A in Caco-2 cells results in global DNA hypomethylation, which is linked to impaired regenerative capacity, transepithelial resistance and intercellular junction formation. (nature.com)
  • In this study, we investigated the pathogenic potential of these marine V. parahaemolyticus strains by studying their adherence ability, their cytotoxicity, their effect on zonula occludin protein 1 (ZO-1) of the tight junctions, and their effect on transepithelial resistance (TER) in infected Caco-2 cells. (umd.edu)
  • HBGA are complex carbohydrates distinguished by different monosaccharides added to a precursor oligosaccharide by fucosyltransferase enzymes ( 2 3, 24 ). (cdc.gov)
  • 24. Wolfrum C, Spener F. Chlorophyll-derived fatty acids regulate expression of lipid metabolizing enzymes in liver, a nutritional opportunity[J]. OCL - Oleagineux Corps Gras Lipides, 2001, 8(1):39-44. (boysandgirlsclubofcypress.com)
  • Archives of Toxicology , 97 (2), 457-468. (wur.nl)
  • Human colon adenocarcinoma (Caco-2) and human embryonic kidney (HEK-293) cells were maintained and treated with various concentrations (0, 20, 40, 80, 100 and 120 mg/l) of K. africana, M. zehyeri and X. caffra seed oil. (iiarjournals.org)
  • We hypothesized that rhythmicity of a key circadian component, PERIOD2 (PER2), would diminish along a continuum from ex vivo intestinal organoids (epithelial 'miniguts'), nontransformed mouse small intestinal epithelial (MSIE) cells and transformed human colorectal adenocarcinoma (Caco-2) cells. (biologists.com)
  • But, when the lower compartment of the Caco2 monolayer was applied to HepG2, some genes were modulated (after 24 h). (nih.gov)
  • Bacteria of the strains EH41 and Ec472/01 were placed on Caco-2 cells for three hours. (thecattlesite.com)
  • These virulence-related genes have previously been detected only in clinical strains. (umd.edu)
  • However, during screening of a collection of environmental V. parahaemolyticus isolates obtained in the North Adriatic Sea in Italy, a number of marine strains carrying virulence-related genes, including genes involved in the T3SS2, were detected. (umd.edu)
  • Our results indicate that, similarly to clinical strains, marine V. parahaemolyticus strains carrying vopT and vopB2 and that other genes included in the VP-PAI are capable of adhering to human cells and of causing cytoskeletal disruption and loss of membrane integrity in infected cells. (umd.edu)
  • A major finding from human NV volunteer studies is that persons with strains containing 2 mutated alleles of the α(1,2) fucosyltransferase ( FUT2 ) gene were resistant to NV infection ( 16 - 20 ). (cdc.gov)
  • Surprisingly, crtE expression had adverse effects on CoQ10 production in both strains. (ijbiotech.com)
  • We hypothesize that early inflammatory mediators, such as chemokines, are released during the muscle disruption and degeneration and are involved in macrophage recruitment and satellite cell migration and activation. (cdc.gov)
  • c-Myc transrepression is mediated by blockade of the transactivating activity of Miz-1, which occurs mainly through a Sp1-binding site in the proximal promoter of the gene. (aacrjournals.org)
  • Src increases NT/N promoter activity in Caco-2 cells acting, in part, through a proximal AP- 1/CRE promoter element. (uky.edu)
  • DNA vaccines are administered in the form of plasmid DNA (pDNA) carrying a strong promoter and the gene of interest. (silverchair.com)
  • Global gene expression analysis reveals differences in cellular responses to hydroxyl- and superoxide anion radical-induced oxidative stress in caco-2 cells. (bvsalud.org)
  • We find that oxidative stress is induced in both cell lines, although at different time points and to different extent. (ntnu.no)
  • DHA induced nuclear translocation of the oxidative stress sensor NFE2L2 in both cell lines, indicating an induction of an anti-oxidative response. (ntnu.no)
  • These results indicate that oxidative stress response is not the cause of DHA-induced cytotoxicity in SW620 cells. (ntnu.no)
  • Semaphorin 4D (Sema4D) belongs to the semaphorin family, which binds to receptors such as Pleixn-B1 (transmembrane structure receptor), CD72 (cluster of differentiation antigen 72, mainly expresses on lymphocyte cells), then activates downstream signaling molecules. (jcancer.org)
  • IL-8 can signal through two receptors, CXCR1 and CXCR2 both of which are members of the G-protein-coupled receptor (GPCR) family [ 2 ]. (biomedcentral.com)
  • BACKGROUND: Colorectal cancer (CRC) is characterised by hypoxia, which activates gene transcription through hypoxia-inducible factors (HIF), as well as by expression of epidermal growth factor (EGF) and EGF receptors, targeting of which has been demonstrated to provide therapeutic benefit in CRC. (ox.ac.uk)
  • In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. (biomedcentral.com)
  • Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
  • Animal-derived oils and purified fatty acids, but not indigenous fruit-tree-derived seed oils, have been used to study cell growth and differentiation. (iiarjournals.org)
  • 1. Peritoneal involvement: Cancer cells can spread to the peritoneum, leading to inflammation and the production of fluid. (researchgate.net)
  • In neutrophils, IL-8 binding to CXCR1 triggers G protein-coupled signalling and formation of second messengers that mediate cellular migration, exocytosis of effectors and a respiratory burst to facilitate oxygen-dependent killing of phagocytosed microorganisms [ 2 ]. (biomedcentral.com)
  • Cellular uptake and receptor specificity were studied in cultures of cod atrial endocardial endothelial cells (aEEC) and head kidney leukocytes. (silverchair.com)
  • Differences in gene expression were investigated by hybridization on two- color microarrays against nonexposed time -matched control cells . (bvsalud.org)
  • Total RNA was isolated and microRNA and target gene expression was compared to the expression in non-infected cells using microRNA microarrays and qRT-PCR. (uni-giessen.de)
  • The mutational inactivation of the TP53 gene in tumor cells has been reported to affect not only tumor cells but also the surrounding cells in the tumor microenvironment and to promote tumor-stromal activation and subsequent tumor growth ( 11 , 12 ). (spandidos-publications.com)
  • Caco-2 cells were infected with Listeria monocytogenes EGD-e, a mutant strain (Delta inlAB or Delta hly) or incubated with purified listeriolysin (LLO). (uni-giessen.de)
  • Interestingly, high SPRY2 expression was related to shorter cancer-specific survival in both KRAS-WT and KRAS-mutant CRC patients. (ncku.edu.tw)
  • It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
  • We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
  • Various genetic abnormalities such as wingless and int-1 (Wnt) signaling, tissue growth factor beta (TGFβ) signaling, or TP53 signaling have been known in the carcinogenesis of colorectal cancer ( 2 , 3 ). (spandidos-publications.com)
  • TP53 is a significant tumor suppressor gene, and its somatic mutations are one of the most frequent alterations in ~50% of all human cancers, including colorectal cancer ( 10 ). (spandidos-publications.com)
  • Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer. (ox.ac.uk)
  • Morphological alterations in the Caco-2 cells were assessed on the basis of scanning electron microscopy at intervals of one, two and three hours. (thecattlesite.com)
  • The factors responsible for the postnatal and starvation-associated induction of HMGCS2 gene expression have been identified and shown to involve changes in circulating hormone and fatty acid levels. (aacrjournals.org)
  • Furthermore, studies have shown HIF-mediated Sema4D induction in the generation of the pro-angiogenic phenotype in oral squamous cell carcinoma (OSCC) and have determined its biological significance for tumor growth and vascularity ( 10 ). (jcancer.org)
  • Induction of Hypoxic Response in Caco-2 Cells Promote the Expression of Genes Involved in SARS-CoV-2 Endocytosis and Transcytosis. (cdc.gov)
  • Caco-2 (ATCC ® HTB-37™) cell cultures treated with VPA (5 mM) or control media were used to confirm changes of H3 acetylation and MiRNA expression. (ecco-ibd.eu)
  • As a proangiogenic response effecter, the interaction of Sema4D with plexin-B1 is dependent on the COOH-terminal PDZ-binding motif of plexin-B1, which binds two guanine nucleotide exchange factors (GEFs) for Rho and is mediated by the activation of Rho-initiated (Ras homolog gene family) pathways ( 4 - 8 ). (jcancer.org)
  • All three tree seed oils suppressed both Caco-2 and HEK-293 cell growth in a dose-dependent manner. (iiarjournals.org)
  • In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
  • The HEK-293 cell line has been used extensively ( 16 - 17 ) as a laboratory work-horse in several cell culture studies. (iiarjournals.org)
  • CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)
  • Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)
  • Recently, a type 3 secretion system (T3SS2) able to secrete and translocate virulence factors into the eukaryotic cell has been identified in a pathogenicity island (VP-PAI) located on the smaller chromosome. (umd.edu)
  • However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
  • Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
  • Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion. (aber.ac.uk)
  • Sodium- and chloride-dependent taurine transporter is a protein that in humans is encoded by the SLC6A6 gene. (wikipedia.org)
  • Reported immunotoxic effects of PFASs include decreased antibody responses in experimental animals and humans, indicating that PFASs may, among others, affect B cell function. (wur.nl)
  • Levels of phosphorylation and protein expression were determined using western blotting. (hindawi.com)
  • HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
  • 50% reduction in NHE2 and NHE3 activities and protein expression. (elsevierpure.com)
  • In rats, region-specific, time- and dose-dependent reductions of NHE2 and NHE3 activities, protein expression, and mRNA were observed after exposure to IFN-γ. (elsevierpure.com)
  • The clinical cohort study revealed a positive association of SPRY2 protein expression with pT status, lymphovascular invasion, and perineural invasion in KRAS-WT CRCs. (ncku.edu.tw)
  • Importantly, the tree seed oils did not cause increased cell death as the number of dead cells remained unchanged under control and oil-treated conditions. (iiarjournals.org)
  • However, expression of 84 angiogenic genes remained unchanged in response to EGF alone. (ox.ac.uk)
  • In the present study, we investigated the anticancer effects of adenine and the underlying mechanism in colon cancer cells. (hindawi.com)
  • Our findings suggest that adenine inhibits the growth of colon cancer cells. (hindawi.com)
  • Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
  • These results suggest that autophagy is important for the DHA sensitivity of colon cancer cells and imply possible therapeutic effects of this fatty acid against cancer cells with low autophagy. (ntnu.no)
  • E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin. (boysandgirlsclubofcypress.com)
  • Therefore, we studied global gene expression by an extensive time series (0.08, 0.25, 0.5, 1, 2, 4, 8, 16, or 24 h) analyses in human colon cancer (caco-2) cells after exposure to H(2)O(2) or the superoxide anion donor menadione . (bvsalud.org)
  • Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
  • Interestingly, PFOA decreased the expression of RAG1 and RAG2, genes involved in immunoglobulin and T cell receptor V(D)J recombination. (wur.nl)
  • These additional genes included chemokines (CCL-11/eotaxin-1 and interleukin-8), collagen type IV α3 chain, integrin β3 chain, TGFα and VEGF receptor KDR. (ox.ac.uk)
  • Fluorescence microscopy of tissue sections from cod injected with fluorescein-labelled pDNA confirmed intracellular uptake of pDNA by the endocardial cells of the atrium and ventricle. (silverchair.com)
  • This two-compartmental model was parameterized to account for nonspecific binding, bidirectional passive diffusion, and active uptake processes based on the physiology of the cells. (aspetjournals.org)
  • Hypoxia is the most common characteristic in the microenvironment of solid tumors because tumor progression and metastasis depend on the ability of cancer cells to initiate angiogenesis and ensure the delivery of oxygen and nutrients ( 11 , 12 ). (jcancer.org)
  • RESULTS: We identified a total of 9 angiogenic genes, including angiopoietin-like (ANGPTL) 4, ephrin (EFNA) 3, transforming growth factor (TGF) β1 and vascular endothelial growth factor (VEGF), to be upregulated in a HIF dependent manner in Caco-2 CRC cells in response to both hypoxia and the hypoxia mimetic dimethyloxallylglycine (DMOG). (ox.ac.uk)
  • In contrast, HIF activation downstream of hypoxia/DMOG drives expression of genes such as ANGPTL4, EFNA3, TGFβ1 and VEGF. (ox.ac.uk)
  • Although DNMT3A and DNMT3B are highly homologous, their redundancy seems to be limited, and their unique biological function, e.g., specificity for methylation patterns in distinct cell types, is still elusive 9 . (nature.com)
  • Distinct microRNA expression depends on both LLO and pathogen localization. (uni-giessen.de)
  • Ancient out of Africa mitochondrial DNA variants associate with distinct mitochondrial gene expression patterns. (mitoeagle.org)
  • Ubiquinone or coenzyme Q (CoQ) is a lipid-soluble molecule of the electron transport chain located in the mitochondrial inner membrane of human cells as well as the cytoplasmic membrane of bacteria. (ijbiotech.com)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • As a next step, time- and concentration-dependent changes in the expression of RAG1 and RAG2 upon exposure to PFOA, PFNA, PFHxS and PFOS were studied through RT-qPCR analysis. (wur.nl)