Gene expression and muscle cell differentiation myod protein. Medical search. Frequent questions

Recent evidence indicates that muscle gene expression is also regulated at the cis level by differential affinity for DNA between MyoD and other E-box binding proteins during myogenesis. (wustl.edu)
C(2)C(12) cells are a standard model for the study of myogenesis. (zfin.org)
These results display a crucial evolutionarily conserved role for Achn in myogenesis and suggest that it plays key roles in the processes of differentiation and self-renewal. (zfin.org)
Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
We focus our research on the post-transcriptional regulation of embryonic myogenesis and adult muscle differentiation by RNA-binding proteins.We use zebrafish as a model, which is most suitable for in vivo and in vitro analyses using cell and molecular biology, genetics, and living imaging approaches. (sorbonne-universite.fr)
Although DMD is a progressive disease, there is growing evidence for early-stage defects in myogenesis and gene expression in DMD. (seattlechildrens.org)
Changes in gene expression can cause defects in myogenesis and muscle regeneration, apoptosis, and sensitivity to oxidative stress. (comprehensivephysiology.com)
a reverse function mutant Talniflumate of Nkx3.2 blocks the ability of Sox9 to both inhibit myogenesis and induce chondrogenesis suggesting that Nkx3.2 is required for Sox9 to promote chondrogenic differentiation in satellite cells. (healthyguide.info)
TNF- TNF- is transiently upregulated in myoblasts inside 3 to 48 h post differentiation induction inside a dose-dependent manner: myogenesis is stimulated at low TNF- concentrations, although is inhibited at higher concentrations [80,81]. (rockinhibitor.com)

Skeletal muscle development is orchestrated by the myogenic regulatory factor MyoD, whose activity is blocked in myoblasts by proteins preventing its nuclear translocation and/or binding to G/C-centered E-boxes in target genes. (wustl.edu)
Canonical Wnt signaling induces Barx2 expression in muscle progenitors and perturbation of Barx2 leads to misregulation of Wnt target genes. (edu.au)
MicroRNAs are small non-coding post-translational biomolecules which, when expressed, modify their target genes. (archivesofmedicalscience.com)
In earlier reports, PRMTs promoted active transcription of target genes by mediating asymmetric dimethylation of arginine residues on histones, resulting in increased acetylation and the subsequent activation of transcription. (e-enm.org)

Skeletal muscle differentiation is triggered by a unique family of myogenic basic helix-loop-helix transcription factors, including MyoD, MRF-4, Myf-5, and Myogenin. (unipv.it)
Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. (vt.edu)
Upon activation satellite cells rapidly initiate MyoD expression which leads to the activation of myogenin and terminally differentiated structural muscle mass genes such as myosin heavy chain (MHC) [1] [3]. (healthyguide.info)

MyoD binds to G/C-centered E-boxes, enriched in muscle differentiation genes, in myotubes but not in myoblasts. (wustl.edu)
Achn expression is induced in myoblasts that form myotubes and acts upstream of the muscle specific transcription factor MyoD. (zfin.org)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)
We find that C/EBPβ can regulate Notch2 expression and that restoration of Notch activity in myoblasts lacking C/EBPβ prevents precocious differentiation. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Loss of C/EBPβ in SCs results in precocious differentiation at the expense of self-renewal in both primary myoblasts and myofiber cultures. (biomedcentral.com)
Ostrovsky O., Bengal, E .*, and Aronheim, A.* (2002) Induction of Terminal Differentiation by the c-Jun Dimerization Protein JDP2 in C2 Myoblasts and Rhabdomyosarcoma Cells . (technion.ac.il)
Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. (vt.edu)
Proliferating myoblasts fuse every single other's within four days in absence of TNF-, whereas TNF- therapies fully inhibit myotube formation and cut down Myog expression. (rockinhibitor.com)
In addition, this proliferating impact is exerted on satellite cells after in vivo TNF- intraperitoneal injection [82], while Myog is lowered confirming differentiation inhibition of this cytokine on myoblasts [85]. (rockinhibitor.com)
IFN- IFN-, a pro-inflammatory cytokine, favors myoblast proliferation, prevents fibrotic events in SkMR, and is expressed by proliferating myoblasts although not by differentiated cells. (rockinhibitor.com)

Regulation of MUC5B Expression in Idiopathic Pulmonary Fibrosis. (nationaljewish.org)
We now identify Barx2, MyoD, and Pax7 as novel components of the Wnt effector complex, providing a new molecular pathway for regulation of muscle progenitor differentiation. (edu.au)
We propose that antagonism between Barx2 and Pax7 in regulation of Wnt signaling may help mediate the switch from myoblast proliferation to differentiation. (edu.au)
Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
The post-transcriptional regulation of gene expression orchestrated by RNA-binding proteins is involved in a wide variety of physiological and pathological processes. (sorbonne-universite.fr)
In particular, we focus our research on the role of RNA-binding proteins in the post-transcriptional regulation of muscle differentiation and regeneration. (sorbonne-universite.fr)
Transcriptional derepression and post-translational regulation of gene expression play important roles in tumorigenesis, but their implication in early development remains elusive. (sorbonne-universite.fr)
We are interested in the networks of gene regulation that control tissue and organ formation in the early vertebrate embryo. (kcl.ac.uk)
The correct regulation of gene activation and repression is crucial both for cell types to become established during embryonic development and for on-going differentiation of stem cells in the adult. (kcl.ac.uk)
Our main focus is on gene regulation during the formation of mesoderm and endoderm - two basic cell types that form in the early embryo. (kcl.ac.uk)
We use a combination of approaches to study gene regulation during cell differentiation including experimental embryology, molecular biology, genomics, proteomics and computational biology in a variety of systems including zebrafish and mammalian cells. (kcl.ac.uk)
Kolpakova A*, Katz S*, Keren A, Rojtblat A, Bengal E . (2013) Transcriptional Regulation of Mesoderm Genes by MEF2D during Early Xenopus Development . (technion.ac.il)
Ciechanover, A., Breitschopf, K., Abu Hatoum, O., and Bengal E. (1999) Degradation of MyoD by the ubiquitin pathway: regulation by specific DNA-binding and identification of a novel site for ubiquitination . (technion.ac.il)
Glucose homeostasis is tightly controlled by the regulation of glucose production in the liver and glucose uptake into peripheral tissues, such as skeletal muscle and adipose tissue. (e-enm.org)

ATGL in beta cells regulates insulin secretion via the production of signalling MAGs. (omicsdi.org)
Estrogen-related receptor alpha (ESRRa) regulates a number of cellular processes including development of bone and muscles. (nature.com)
Thus, our objective is to understand how RBM24 regulates protein homeostasis during muscle cell differentiation and regeneration. (sorbonne-universite.fr)
We demonstrated that XDSCR6, a Xenopus homolog of human Down syndrome critical region protein 6, regulates mesoderm and embryonic axis formation through derepression of polycomb group (PcG) proteins, including Ezh2. (sorbonne-universite.fr)
The 2'-5' oligoadenylate/RNase L/RNase L inhibitor pathway regulates both MyoD mRNA stability and muscle cell differentiation. (nih.gov)

This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
Inhibition of EHMT2 expression or activity in human ERMS cell lines reduced DKK1 expression and elevated canonical Wnt signaling resulting in myogenic differentiation in vitro and in mouse xenograft models in vivo. (elifesciences.org)
Lately, in vitro differentiation of human being embryonic stem cells proven that Wnt/-catenin signaling takes on an important part in releasing early genes that are necessary for NC advancement [22]. (globaltechbiz.com)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)

Here, we used cell-based and in vivo Drosophila, Xenopus laevis, and mouse models to show that ZEB1, a G/C-centered E-box binding transcriptional repressor, imposes a temporary stage-dependent inhibition of muscle gene expression and differentiation via CtBP-mediated transcriptional repression. (wustl.edu)
Inhibition of muscle genes by ZEB1 occurs via transcriptional repression and involves recruitment of the CtBP corepressor. (wustl.edu)
Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
B) complete inhibition of transcription of the structural gene controlled by that regulator. (easynotecards.com)
This theory emphasizes the abnormal intracellular accumulation of many proteins, protein aggregation and misfolding, proteosome inhibition, and endoplasmic reticulum (ER) stress. (wikidoc.org)
Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. (nih.gov)
Alter, J., Rozentzweig, D., and Bengal, E. (2008) Inhibition of myoblast differentiation by tumor necrosis factor alpha is mediated by c-Jun N-terminal kinase 1 and leukemia inhibitory factor . (technion.ac.il)
IFN- stimulation impairs myoblast fusion and differentiation gene expression, most likely by way of inhibition of Myog expression by Class II Main Histocompatibility Complex transactivator (CIITA). (rockinhibitor.com)
Even so, this inhibition is reversible as CIITA is swiftly downregulated, and muscle-specific genes upregulated [86,87]. (rockinhibitor.com)

Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor-kappaB inflammatory signaling pathway in human skin cells. (nationaljewish.org)
In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)
MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
Loss of function analysis shows that knockdown of esrra impairs expression of genes including sox9, col2a1, sox5, sox6, runx2 and col10a1 thus induces abnormally formed cartilage in pharyngeal arches. (nature.com)
Tamir, Y., and Bengal E . (2000) Phosphoinositide 3-kinase induces the transcriptional activity of MEF2 proteins during muscle differentiation . (technion.ac.il)

Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)
These transcription factors bind promoters and distant regulatory regions, including E-box elements, of genes whose expression is restricted to muscle cells. (unipv.it)
how Wnt signals integrate with muscle-specific regulatory factors in this process is poorly understood. (edu.au)
We previously demonstrated that the Barx2 homeobox protein promotes differentiation in cooperation with the muscle regulatory factor (MRF) MyoD. (edu.au)
3) Which of the following is a protein produced by a regulatory gene? (easynotecards.com)
7) A mutation that inactivates the regulatory gene of a repressible operon in an E. coli cell would result in A) continuous transcription of the structural gene controlled by that regulator. (easynotecards.com)
Recent basic and clinical research has revealed that hydrogen is an important physiological regulatory factor with antioxidant, anti-inflammatory and anti-apoptotic protective effects on cells and organs. (researchgate.net)
The backbone of the sarcomere is composed of three filament systems: the myosin-based thick filament, the actin-based thin filament, supplemented with the regulatory protein tropomyosin and the troponin complex, and the titin filament. (frontiersin.org)
TNF- could possibly be also involved in muscle strength recovery, most likely via modulation of muscle regulatory gene expression, including MyoD [80,84]. (rockinhibitor.com)

Mostafa MM, Bansal A, Michi AN, Sasse SK, Proud D, Gerber AN , Newton R. Genomic determinants implicated in the glucocorticoid-mediated induction of KLF9 in pulmonary epithelial cells. (nationaljewish.org)
The REAC action bypassed a persistent reprogramming toward an induced pluripotent stem cell-like state and involved the transcriptional induction of the NADPH oxidase subunit Nox4. (nih.gov)
The need for other pathways continues to be being researched: Notch signaling participation was founded through studies where gain- or loss-of-function of Notch signaling or the Notch effectors, genes, had been associated with standards, nC or induction migration [23,24,25,26]. (globaltechbiz.com)
The purpose of this function was to Azlocillin sodium salt determine whether HES-1 and MSX-1 take part in the induction/differentiation from the NC using pluripotent mammalian ESCs like a model. (globaltechbiz.com)
3 contains a cell surface receptor site for thyroid hormone that is linked to activation of mitogen-activated protein kinase and induction of angiogenesis. (biologie-journal.org)

Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
We investigate the role of Zeb2 in skeletal muscle differentiation using genetic tools and transgenic mouse embryonic stem cells, together with single-cell RNA-sequencing and in vivo muscle engraftment capability. (unipv.it)
In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
BMP signaling is pertinent during NC differentiation in vivo. (globaltechbiz.com)
in vivo, cytokines expression, promoted tissue clearance and its regeneration. (rockinhibitor.com)

Expression levels of mouse Akt2 mRNA and Akt2 protein varied among tissues, with the highest levels in skeletal muscle. (nih.gov)
These data demonstrate that Akt2 expression is activated during cellular differentiation and suggest that it functions in the signaling pathways of some adult tissues. (nih.gov)
The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
The manipulation of fat stores is an obvious therapeutic dream, but disruption of the normal differentiation or development of white adipose tissues (WAT) causes ectopic lipid storage and severe pathology (lipodystrophy) in both humans and experimental animals. (bioseek.eu)
Here, we show that the transcription factor zinc finger E-box-binding homeobox 2 (Zeb2, Sip-1, Zfhx1b) is present in skeletal muscle tissues. (unipv.it)
Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
Expression levels of adipogenic and lipogenic genes in adipose tissues were also dramatically reduced by Lkb1 deletion. (omicsdi.org)
A complex expression pattern of ESRRs during animal development seems to be consistent with the potential roles for ESRRa during appropriate developmental programs of tissues and organs in mouse and zebrafish 9 . (nature.com)
However, aside from muscle development, the roles of ESRRs in other tissues including bone and cartilage have just begun to be investigated 10 . (nature.com)
Deficiency in its expression level could be the cause of congenital disorders, such as cardiomyopathy, myopathy or blindness, which affect the normal function of related tissues where crucial roles of this gene have been demonstrated in different animal models. (sorbonne-universite.fr)
Cells specified as mesoderm at early stages will go on to form tissues such as blood, skeletal muscle, cardiac muscle, cartilage and bone, whereas endoderm cells will form liver, lung, pancreas and gut. (kcl.ac.uk)
By identifying the programs of gene expression that lead to the formation of these cells types we hope to be able to recapitulate them in the lab and generate cells that could be used for repair and replacement of damaged tissues in humans. (kcl.ac.uk)
Expression of mRNAs of pancreatic and L type RNase inhibitors as a function of age in different tissues of SAMP8 and BDF1 mice. (nih.gov)
Under prolonged fasting, hepatic gluconeogenesis is mainly responsible for glucose production in the liver, which is essential for tissues, organs, and cells, such as skeletal muscle, the brain, and red blood cells. (e-enm.org)
IFN- expression is at basal levels in wholesome muscle tissues, although increases just after injury, peaking at day 5 post-injury corresponding to immune cell and myoblast infiltration. (rockinhibitor.com)

Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
FSHD candidate genes on chromosome 4q35. (comprehensivephysiology.com)
Locations of the candidate genes and the DME1 and DME2 myogenic transcription enhancers (92) are shown. (comprehensivephysiology.com)

Somatic cells can be directly reprogrammed to alternative differentiated fates without first becoming stem/progenitor cells. (nih.gov)
Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and to self-renew. (biomedcentral.com)
Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)
We show that Zeb2 over-expression has a positive impact on skeletal muscle differentiation in pluripotent stem cells and adult myogenic progenitors. (unipv.it)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
Bengal E*, Perdiguero E, Serrano AL, Muñoz-Cánoves P* (2017) Rejuvenating stem cells to restore muscle regeneration in aging . (technion.ac.il)
Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
Intro Satellite cells are the cells specific stem cells in the adult skeletal muscle mass. (healthyguide.info)
In the beginning satellite cells were considered to Talniflumate be unipotent stem cells with the ability of generating a unique specialized phenotype the skeletal muscle mass cells. (healthyguide.info)

Its knockdown results in precocious expression of muscle differentiation genes and acceleration of myotube formation. (wustl.edu)
We find that loss of C/EBPβ leads to precocious differentiation at the expense of self-renewal in primary myoblast and myofiber cultures. (biomedcentral.com)
Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development. (vt.edu)

Davis P.J., Shih A., Lin H.Y., Martino L.J. & Davis F.B., Thyroxine promotes association of mitogen-activated protein kinase and nuclear thyroid hormone receptor (TR) and causes serine phosphorylation of TR. (biologie-journal.org)
Zetser, A., Gredinger, E., and Bengal, E. (1999) p38 mitogen-activated protein kinase promotes skeletal muscle differentiation: participation of the MEF2C transcription factor . (technion.ac.il)

Specifically,treatment of primary limb bud cultures with p38 inhibitors increases the expression of myogenic markers and causes a striking increase in formation of myotubes, which were detected using antibodies specific for myosin heavy chain. (biologists.com)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)

Sequence analysis revealed that, like its human homolog, Akt2 encodes a protein-serine/threonine kinase containing a pleckstrin homology domain at its amino terminus. (nih.gov)
Liver kinase B1 inhibits smooth muscle calcification via high mobility group box 1. (omicsdi.org)
Tamir, Y., and Bengal, E. (1998) p53 protein is activated during muscle differentiation and participates with MyoD in the transcription of muscle creatine kinase gene . (technion.ac.il)

The MyoD gene was duplicated during the teleost whole genome duplication and, while a second MyoD gene ( MyoD2 ) was subsequently lost from the genomes of some lineages (including zebrafish), many fish lineages (including Alcolapia species) have retained both MyoD paralogues. (mdpi.com)
Expression of esrra is colocalised with genes necessary for cartilage development in pharyngeal arches during zebrafish embryogenesis. (nature.com)
We are using CRISPR genome screening and precision editing in zebrafish to engineer mutations in genes that have been implicated in human congenital heart defects. (seattlechildrens.org)
We recently identified novel epigenetic small molecules that improve muscle degeneration in DMD zebrafish ( https://doi.org/10.1186/s13395-020-00251-4 ). (seattlechildrens.org)
We are using zebrafish and mouse models to study the functions of factors such as Pbx homeodomain transcription factors and how they control muscle fiber-type development. (seattlechildrens.org)
We take advantage of zebrafish models to address whether factors that regulate fiber-type differentiation enhance or suppress the zebrafish dmd muscle degeneration phenotype. (seattlechildrens.org)

Other E-box binding zinc finger proteins target the same DNA response elements, however, their function in muscle development and regeneration is still unknown. (unipv.it)
We therefore propose that Zeb2 is a novel myogenic regulator and a possible target for improving skeletal muscle regeneration. (unipv.it)
Thus, our aim is to decipher the post-transcriptional mechanisms mediated by RBM24 in muscle development and regeneration. (sorbonne-universite.fr)
Furthermore, we found that RBM24 displays dynamic subcellular localization and post-transcriptional functions during muscle differentiation and regeneration. (sorbonne-universite.fr)
Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
After a second round of injury, muscle regeneration is impaired in C/EBPβ conditional knockout mice compared to wild-type control mice. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
Further, C/EBPβ-deficient SCs are unable to self-renew after muscle injury leading to a reduction in the SC pool such that it cannot efficiently support muscle regeneration after a second round of injury. (biomedcentral.com)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)

Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)

Recently, several chromatin-related proteins have been shown to regulate ESC pluripotency and/or differentiation, yet the role of the major heterochromatin proteins in pluripotency is unknown. (biomedcentral.com)
It is estimated that microRNAs regulate production of approximately 60% of all human proteins and enzymes that are responsible for major physiological processes. (archivesofmedicalscience.com)
Importantly, we identify putative ESRRa binding elements in upstream regions of sox9 to which ESRRa can directly bind, indicating that Esrra may directly regulate sox9 expression. (nature.com)
We are also working to identify new epigenetic factors that regulate muscle fiber type. (seattlechildrens.org)
CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)

RNA-binding proteins (RBPs) control RNA metabolism at multiple levels, and are critical for maintaining the homeostasis of protein synthesis during early development and in adult life. (sorbonne-universite.fr)

There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
Satellite cells from your trunk and the limb are derived from an embryonic populace of progenitor cells in the somites transient mesodermal constructions that develop on either part of the neural tube [1]. (healthyguide.info)

The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
In multipotent ectodermal cells (pet hats), a BMP focus similar compared to that necessary to induce the NC improved levels [29]. (globaltechbiz.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Odeh M, Tamir-Livne Y, Haas T, Bengal E (2019) P38α MAPK coordinates the activities of several metabolic pathways that together induce atrophy of denervated muscles . (technion.ac.il)

Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
The balance between differentiation and self-renewal is critical to maintain muscle tissue homeostasis, and alterations in this equilibrium can lead to chronic muscle degeneration. (biomedcentral.com)

Keren, A., Tamir , Y., and Bengal, E . (2006) The p38 MAPK signaling pathway: A major regulator of skeletal muscle development . (technion.ac.il)

We found that, contrary to MyoD, ZEB1 binds to G/C-centered E-boxes in muscle differentiation genes at the myoblast stage but not in myotubes. (wustl.edu)
The minor fraction of HP1β that is chromatin-bound in ESCs is enriched within exons, unlike the situation in differentiated cells, where it binds heterochromatic satellite repeats and chromocenters. (biomedcentral.com)
Among these, EZH2 that mediates repressive histone H3 lysine 27 trimethylation (H3K27me3) is upregulated and binds to muscle specific genes in ERMS. (elifesciences.org)
5) Which of the following, when taken up by the cell, binds to the repressor so that the repressor no longer binds to the operator? (easynotecards.com)
B) Allolactose binds to the repressor protein. (easynotecards.com)
C) Allolactose binds to the regulator gene. (easynotecards.com)

In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. (vt.edu)

A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
In TNF- receptor double-knockout mice, p38 MAPK expression diminishes collectively with MyoD-1, a proliferation marker, in TNF- deficient mice [84]. (rockinhibitor.com)

Fasting signals are relayed by various intracellular enzymes, such as kinases, phosphatases, acetyltransferases, and deacetylases, which affect the transcriptional activity of transcription factors and transcriptional coactivators for gluconeogenic genes. (e-enm.org)

Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)
Moreover, Barx2 interacts with the core Wnt effectors β-catenin and T cell-factor 4 (TCF4), is recruited to TCF/lymphoid enhancer factor sites, and promotes recruitment of β-catenin. (edu.au)
Beside PGC1a/b, ESRRa has been shown to potentiate a metabolic syndrome by acting downstream of mammalian target of rapamycin (mTOR) 7 and also promotes hypoxic adaptation of cancer cells by stabilizing hypoxia inducible factor 1-alpha (HIF1-a) from degradation 8 . (nature.com)

As a brand new graduate student starting in October 1956, my supervisor Michail Fischberg, a lecturer in the department of Zoology at Oxford, suggested that I should try to make somatic cell nuclear transplantation work in the South African frog Xenopus laevis . (biologists.com)
Even advanced donor cells from the endoderm of Xenopus tadpoles have nuclei that can sometimes yield normal individuals after nuclear transfer [data taken from Briggs and King ( Briggs and King, 1957 ) for Rana and from Gurdon ( Gurdon, 1962 ) for Xenopus ]. (biologists.com)

This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
Our team is interested in understanding the molecular and cellular mechanisms that control muscle development in vertebrate embryos. (sorbonne-universite.fr)
In addition, it displays dynamic subcellular localization during cellular differentiation. (sorbonne-universite.fr)
Analyses of RBM24 loss of function reveal that it is critically required for cellular differentiation. (sorbonne-universite.fr)
DUX4 misexpression dysregulates diverse cellular activities that may contribute to FSHD muscle pathology. (comprehensivephysiology.com)
ABC proteins transport various molecules across extra- and intra-cellular membranes. (nih.gov)

Muscle samples were collected and myogenic cells were isolated and cultivated. (nature.com)
DUX4 expression was visualized in differentiated 17ABic myogenic cells (104) using an integrated DUX4‐GFP reporter (198). (comprehensivephysiology.com)
Macrophages expressing pro-inflammatory markers are abundant in regenerating regions damaging for Myog (a transcription aspect expressed only in differentiated myogenic cells) suggesting various associations depending on proliferation or differentiation of myogenic precursors [78,79]. (rockinhibitor.com)

Akt2 expression was low in a multipotent fibroblast cell line, but it was upregulated when these cells were transformed with Myod and induced to differentiate into myocytes. (nih.gov)
Tamir-Livne Y, Mubariki R, Bengal E . (2017) Adhesion molecule Kirrel3/Neph2 is required for the elongated shape of myocytes during skeletal muscle differentiation . (technion.ac.il)
Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)

Here, we provide evidence that exposure of human skin fibroblasts to a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering radio electric conveyed fields at a radiofrequency of 2.4 GHz, afforded remarkable commitment toward cardiac, neuronal, and skeletal muscle lineages. (nih.gov)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

Conversely, REAC treatment elicited a biphasic effect on a number of stemness-related genes, leading to early transcriptional increase of Oct4, Sox2, cMyc, Nanog, and Klf4 within 6-20 h, followed by a downregulation at later times. (nih.gov)
Conversely, deregulation of gene expression may lead to cancerous changes and other diseases. (kcl.ac.uk)

Altonsy MO, Mostafa MM, Gerber AN , Newton R. Long-acting beta2-agonists promote glucocorticoid-mediated repression of NF-kappaB by enhancing expression of the feedback regulator TNFAIP3. (nationaljewish.org)
A) The repressor protein attaches to the regulator. (easynotecards.com)
Silent information regulator 2 (Sir2) proteins, or sirtuins, are protein deacetylases dependent on nicotine adenine dinucleotide (NAD) and are found in organisms ranging from bacteria to humans. (biomedcentral.com)

The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. (vt.edu)

With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)

The primary purpose of this study is to determine whether breast cancer tumors respond (as measured by pathologic complete response: the absence of microscopic evidence of invasive tumor cells in the breast) to combined chemotherapy of AC(doxorubicin and cyclophosphamide) followed by paclitaxel plus trastuzumab or lapatinib or both given before surgery to patients with HER2-positive breast cancer. (stanford.edu)
Also, the study will look at whether there are gene expression profiles in the tumor tissue that can predict pathologic complete response. (stanford.edu)
RATIONALE: Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die. (stanford.edu)
In addition, growing evidence has indicated that LKB1 functions as a tumor suppressor gene. (omicsdi.org)

Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)

When deprived of growth factors, these cells can be induced to: form multinucleated myotubes, arrest as quiescent satellite-like reserve cells, or undergo apoptosis. (zfin.org)
Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
These cells lay beneath the basement membrane of the muscle mass fiber and are usually mitotically quiescent [1]. (healthyguide.info)
Interestingly recent data indicated that although MyoD is not indicated in quiescent satellite cells in the adult it is transiently indicated in satellite cell progenitors in the embryo suggesting that satellite cells are derived from committed embryonic precursors of myogenic lineage [4] [5]. (healthyguide.info)

The two DUX4‐fl isoforms produce the same protein sequence and differ only in the splicing of the 3′UTR. (comprehensivephysiology.com)
Differential splicing of the titin gene results in numerous species- and muscle-specific titin isoforms. (frontiersin.org)

Accordingly, ectopic expression of sox9 rescues defective formation of cartilage induced by the knockdown of esrra . (nature.com)
Ectopic expression of either Pax3/Zic1 or Snai2/Wnt8 made gastrula explants qualified to produce myogenic differentiation 1 (MyoD) and Endodermin. (globaltechbiz.com)

The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
DUX4‐fl transcription factor misexpression in skeletal muscle leads to disrupted RNA splicing, nonsense mediated decay (NMD), and protein degradation, possibly causing damaged associated molecular pattern (DAMP) release, which may elicit an immune response to targeted damaged muscles. (comprehensivephysiology.com)

Am J Respir Cell Mol Biol. (nationaljewish.org)
Front Cell Dev Biol 2020). (sorbonne-universite.fr)
Cell Biol. (biologie-journal.org)
Mol Cell Biol, 2000 Jul. (nih.gov)

One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)
We found that in the descendents of muscle mass progenitors that contributed to cartilage formation Nkx3.2 and Sox9 were strongly induced while Pax3 manifestation was strongly repressed. (healthyguide.info)

Single cell RNA sequencing identifies TGFbeta as a key regenerative cue following LPS-induced lung injury. (nationaljewish.org)
Our results show for the first time the feasibility of using a physical stimulus to afford the expression of pluripotentiality in human adult somatic cells up to the attainment of three major target lineages for regenerative medicine. (nih.gov)

Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
We are investigating the mechanisms of skeletal muscle fiber-type differentiation. (seattlechildrens.org)
One focus of our lab is understanding the mechanisms behind how skeletal muscle cells differentiate into a specific muscle fiber type, either fast twitch or slow twitch. (seattlechildrens.org)
Protein-interactions e.g., with muscle ankyrin repeat proteins or muscle LIM-protein link titin to hypertrophic signaling and via p62 and Muscle Ring Finger proteins to mechanisms that control protein quality control. (frontiersin.org)

The evolutionary history of MyoD1 and MyoD2 is compared to the presence of this polyserine region using phylogenetics, and its functional relevance is tested using overexpression in a heterologous system to investigate subcellular localisation, stability, and activity of MyoD proteins that include and do not include the polyserine region. (mdpi.com)

Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. (biomedcentral.com)
Frontiers in Cell and Developmental Biology. (kcl.ac.uk)

FSHD is caused by a genetic mutation leading to deregulation of the DUX4 gene. (wikipedia.org)
How this genetic modulation causes muscle damage remains unclear. (wikipedia.org)
The DUX4 gene was discovered in 1999, found to be expressed and toxic in 2007, and in 2010 the genetic mechanism causing its expression was elucidated. (wikipedia.org)
We expect that this project will identify genetic and epigenetic regulators of muscle fiber-type identities that confer susceptibility or resistance to muscular dystrophy. (seattlechildrens.org)

Anatomy20

Organisms5

Chemicals and Drugs23

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes30

Disciplines and Occupations1

Information Science3

Myogenesis11

Target genes4

Myogenin3

Myoblasts12

Regulation16

Regulates5

Vitro6

Inhibition9

Induces5

Regulatory9

Induction5

VIVO6

Tissues16

Candidate genes3

Stem18

Precocious3

Mitogen-activa2

Myosin3

Kinase3

Zebrafish6

Regeneration11

Inhibits1

Regulate5

Metabolism1

Progenitor cells4

Induce5

Self-renewal2

Signaling pathway1

Binds6

Proliferation and differentiation1

Mice3

Transcriptional activity1

Promotes3

Xenopus2

Cellular7

Myogenic cells3

Myocytes3

Fibroblasts2

Conversely2

Regulator3

Skeletal muscle development1

Mammalian1

Tumor4

Pluripotent cells1

Quiescent4

Isoforms2

Ectopic expression2

Transcription factor2

Biol4

Progenitors2

Regenerative2

Mechanisms4

Stability1

Developmental2

Genetic4