Gene expression and apoptosis induction leukemic cells. Medical search. Frequent questions

The up-regulated proteins from BC-derived uEVs were enriched in pathways related to cancer progression (i.e., cell proliferation, cell survival, cell cycle, cell migration, carbohydrate metabolism, and angiogenesis). (bvsalud.org)
Since these six proteins often act as markers of cell proliferation and progression, they may be potential biomarkers for BC screening and diagnosis. (bvsalud.org)
proliferation and cell cycle phase distribution, (2). (nih.gov)
Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
There is not cured for MM. Tan II A has been reported to reduce cancer cell proliferation. (biomedcentral.com)
We sought to explore the possible mechanism by which Tan II A regulated cell proliferation through autophagy signaling pathway in A375 cells. (biomedcentral.com)
Cell proliferation was assessed by using methyl thiazol tetrazolium (MTT) assay. (biomedcentral.com)
We found that Tan IIA inhibited melanoma A375, MV3, and M14 cell proliferation in dose and time dependent manner. (biomedcentral.com)
We concluded that Tan II A reduced A375 cells proliferation by activation of autophagy production, blocked PI3K- Akt - mTOR - p70S6K1 signaling pathway, increased autophagic related gene beclin-1, LC3-II protein expressions and induced autophagocytosis. (biomedcentral.com)
In esophageal cancer, radiation therapy was found inducing autophagy in cancer cells, promoting cancer cell proliferation and causing treatment resistance [ 10 ]. (biomedcentral.com)
Other reports suggested that autophage suppressed cancer development in early stage and promoted cancer cell proliferation in later stage [ 4 ]. (biomedcentral.com)
Knockdown of miR-128a and/or miR-130b significantly impaired proliferation of human MLL-AF4 + cell lines, and inhibition of miR-130a significantly impaired disease initiation by the pediatric MLL-AF4 + SEM ALL cell line in immunodeficient NOD- scid IL2Rgamma null (NSG) mice. (ashpublications.org)
overexpression of these genes decreased proliferation or induced apoptosis of human MLL-AF4 + ALL cell lines, respectively. (ashpublications.org)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
Research has shown that cannabis possesses anti-neoplastic properties, meaning that it can inhibit or halt the development of cancerous tumors by initiating cell death, limiting inflammation, cell proliferation, and cell survival. (nanocraftcbd.com)
Although all forms of leukemia share similarities such as excessive white blood cell production within the blood indicating a heightened immune response and cellular mutation and proliferation, each subtypes posses their own particular traits. (nanocraftcbd.com)
Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
Overexpression of TRIM13 in NCI-H1975 and SPC-A-1 cells hampered cell proliferation. (cancerindex.org)

As the SEC can regulate the transcriptional activity of RNA polymerase II, it was hypothesized that these MLL-fusions induce aberrant regulation of transcriptional elongation on MLL-target genes 14 . (nature.com)
Both compounds induced up-regulation of proapoptotic BH3-only proteins Bim and Puma, and subsequent cell death. (ashpublications.org)
FLT3 (FMS-like tyrosine kinase 3) is a type III receptor tyrosine kinase (RTK) closely related to the platelet-derived growth factor (PDGF) receptor and c-Kit with important functions in the regulation of early hematopoietic cells. (ashpublications.org)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
The key interests lie within the field of genome integrity and variation, cell division, regulation of gene expression, protein turnover and signal transduction. (ki.se)
We examined not just the effect of proadifen and MTX on the expression of BCRP, but we were also interested in other molecular mechanisms involved in the possible antitumour activity of proadifen alone and in combination with MTX, such as the expression of anti-apoptotic proteins, proteins involved in the regulation of BCRP and proteins involved in the reparation of chemotherapeutic drug-induced DNA damage. (spandidos-publications.com)
These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)

SETD2 loss causes growth arrest and differentiation of AML cells, and leads to increased DNA damage. (nature.com)
SETD2 loss synergizes with pharmacologic inhibition of the H3K79 methyltransferase DOT1L to induce DNA damage, growth arrest, differentiation, and apoptosis. (nature.com)
We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
Exposure of sensitive cell lines with PFI-1 results in G1 cell-cycle arrest, downregulation of MYC expression, as well as induction of apoptosis and induces differentiation of primary leukemic blasts. (ox.ac.uk)
This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. (cancerindex.org)
This research aims at characterizing molecules and signals involved in controlling the undifferentiated state and self-renewal capacity of stem cells, and to develop methods to direct the differentiation of stem cells into clinically relevant cell types. (ki.se)
Basic genetic and molecular biology methodologies are combined with complementary bioinformatic and genome-wide approaches, allowing investigation global molecular changes in cells in various differentiation processes. (ki.se)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
In 1974, Leo Sachs pioneered research on leukemic cell differentiation in vivo. (medscape.com)
CSCs take charge of a cancer hierarchy, harboring stem cell-like properties involving self-renewal and aberrant differentiation potential. (bmbreports.org)

However, better understanding of the role of increased JAK-STAT signaling [either through activating mutations ( JAK2 , MPL515L/K ) within the signaling pathway, or mutations involving CALR ], the role of deregulated pro-inflammatory cytokine expression, and the impaired bone marrow microenvironment is transforming the treatment approach for MF. (haematologica.org)
Mechanistically, the CCL20/CCR6 signaling pathway upregulates HIF-1α by stimulating nuclear factor kappa B-driven transactivation of the HIF1A gene. (cancerindex.org)
It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
KEGG enrichment and gene set enrichment analysis (GSEA) showed that a large number of genes were enriched in inflammatory immune-related pathways, such as the toll-like receptor signaling pathway. (frontiersin.org)
The integrated stress response is a signaling pathway activated in cancer cells in response to both dysregulated growth and metabolism, and also following exposure to many therapies that appears one such targetable pathway for improved treatment of these diseases. (biomedcentral.com)
The ISR (Fig. 1 ) is a complex signaling pathway that regulates cellular responses to stress stimuli and enables either adaptation or the instigation of cell death mechanisms [ 2 ]. (biomedcentral.com)

Flow cytometric analysis revealed partial cell arrest in the G(1) phase at less than 5 micro L/mL and induction of apoptosis at 10 micro L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. (nih.gov)
Similar findings were made in bone marrow cells from gene-targeted mice lacking Bim and/or Puma infected with FLT3-ITD and treated with inhibitor, where loss of Puma only provided transient protection from apoptosis, but loss of Bim preserved clonal survival upon FLT3-ITD inhibition. (ashpublications.org)
Solanum nigrum (SN) is a herb with anticancer effects such as destruction of tumor cell membrane, induction of apoptosis by NF-kappaB, caspase activation, nitric oxide production and inhibition of angiogenesis [ 3 ]. (ijpsonline.com)
Intriguingly, cells exposed to PFI-1 showed significant downregulation of Aurora B kinase, thus attenuating phosphorylation of the Aurora substrate H3S10, providing an alternative strategy for the specific inhibition of this well-established oncology target. (ox.ac.uk)
The acquired resistance was associated with subclonal amplification of MET and increased MET expression, suggesting that inhibition of EGFR and BRAF selected for MET -amplified clones. (aacrjournals.org)
We show for the first time that proadifen is able to enhance the cytotoxic properties of MTX in cBCRP cells, particularly through the inhibition of BCRP expression and activity. (spandidos-publications.com)
This proadifen-MTX synergism was also mediated by the inhibition of various cellular proteins engaged in apoptosis, including Mc-1, Bcl-xL, survivin and activation of procaspase-3. (spandidos-publications.com)
Moreover, we revealed the ability of proadifen to increase the intracellular hypericin content in HT-29 cells most likely via the inhibition of BCRP and MRP1 transporters ( 8 ). (spandidos-publications.com)

Similar to MLL-AF4 + human ALL, pMIRH-128a - and pMIRH-130b -transplanted mice showed significant leptomeningeal tumor cell infiltration. (ashpublications.org)
However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
Studies have suggested that statins may inhibit tumor cell growth and possibly prevent carcinogenesis. (cdc.gov)
More than 15 years ago, cholesterol decrement was first shown to inhibit tumor cell growth, metastasis of tumor cells, and induction of apoptosis (7). (cdc.gov)

In addition to its role in H3K36 tri-methylation, SETD2 is required to maintain high H3K79 di-methylation and MLL-AF9-binding to critical target genes, such as Hoxa9 . (nature.com)
Comparative transcriptomic analysis of tumor cells from diseased mice revealed the expression of several well-known MLL-rearranged leukemia-associated target genes, including Meis1 , Runx1 ( pMIRH-128a mice), HoxA9 , Flt3 ( pMIRH-130b mice), Cdk6 , and Bcl2 . (ashpublications.org)
Malouf et al also identified several candidate target genes that may mediate the leukemic activity of miR-128 (including the nuclear orphan receptor NR2F6 gene), miR-130b, or both (including the sphingomyelin synthase 1 [ SGMS1 ] gene). (ashpublications.org)

1 Mixed lineage leukemia (MLL) gene fusions are the molecular hallmark of infant acute lymphoblastic leukemia (ALL) and are present in tumor cells in up to 80% of patients. (ashpublications.org)
T-cell acute lymphoblastic leukemia (T-ALL) arises from leukemic transformation and expansion of immature T-cell precursors. (aacrjournals.org)
Acute lymphocytic leukemia (ALL) , also called acute lymphoblastic leukemia and acute lymphoid leukemia, is a blood cancer that results when abnormal white blood cells (leukemia cells) accumulate in the bone marrow. (nanocraftcbd.com)

The expression of autophagy-associated protein beclin-1 and microtubule-associated protein 1 light chain 3(LC3)-II, as well as phosphatidylinositol 3-kinase(PI3K)、protein kinase B (Akt)、mammalian target of rapamycin (mTOR)、p70S6K1 signaling pathways were detected by using Western blotting. (biomedcentral.com)
Tan IIA promoted autophagic body production and increased autophagy-associated protein beclin-1 and LC3-II expression in A375 cells. (biomedcentral.com)
The host cell exerts its self-clearing of toxic substances such as damaged proteins and organelles through autophagy processes [ 7 ]. (biomedcentral.com)
Autophagy plays an important role in cell growth, development and disease suppression. (biomedcentral.com)
When the cell's DNA and protein damaged, the cells maintained its cellular homeostasis through autophagy. (biomedcentral.com)
It has been reported that the reduction of autophagy-related gene expressions in skin melanoma [ 8 ]. (biomedcentral.com)
Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
Low expression of the autophagy initiator ULK1 in AML can partially be attributed to high expression of the ULK1-targeting microRNA-106a. (hindawi.com)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
Studies in yeast have identified a series of autophagy- (ATG-) related genes forming the autophagy machinery. (hindawi.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
Moreover, genome instability was attributed to impaired autophagy and several autophagy genes with tumor suppressor functions (e.g. (hindawi.com)
In this investigation, the role of autophagy and apoptosis in Solanum nigrum fruit extract-induced cytotoxicity in MDA-MB-468, a triple negative breast cancer cell line was studied. (ijpsonline.com)
Autophagy was detected using acridine orange staining and real-time PCR for Lc3 and Beclin1 genes expression. (ijpsonline.com)
The expression levels of autophagy-related genes, Lc3 and Beclin1 increased in cells treated with 1.5 mg/ml of Solanum nigrum extract. (ijpsonline.com)
These findings indicated that Solanum nigrum extract induced cell death in MDA-MB-468 cells by two distinct mechanisms, apoptosis and autophagy, and these findings further suggest a possibility that this extract could be used to treat triple negative breast cancer. (ijpsonline.com)
A previous study reported that the extract of the whole plant of SN activated cell death in hepatoma cells through autophagy and apoptosis [ 7 ]. (ijpsonline.com)
Autophagy and apoptosis are two forms of programmed cell death, which are essential for cellular homeostasis. (ijpsonline.com)
Autophagy, the type II cell death, is a physiological mechanism involving degradation of intracellular damaged proteins and organelles into membrane vacuoles. (ijpsonline.com)
Autophagy is a membrane trafficking pathway that cells activate under diverse stress conditions to deliver cytosolic material for degradation to lysosomes. (lifescienceevents.com)

Bromo and extra terminal (BET) proteins (BRD2, BRD3, BRD4, and BRDT) are transcriptional regulators required for efficient expression of several growth promoting and antiapoptotic genes as well as for cell-cycle progression. (ox.ac.uk)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
Therefore, we investigated the role of infiltrating B cells in LUAD progression and its correlation with PD-L1 expression. (cancer-genetics.org)
Many studies reported that some semaphorins are involved in the development of a permissive tumor niche, guiding cell-cell communication and, consequently, the development and progression, as well as the response to therapy, of different cancer histotypes, including melanoma. (biomedcentral.com)
Moreover, semaphorins and their cognate receptors are involved in controlling cancer stem cell phenotype, contributing to tumor progression, resistance to therapies, and metastasis initiation [ 18 ]. (biomedcentral.com)

3 Detection of the MLL-AF4 fusion protein in CD34 + /CD19 − fetal hematopoietic stem or progenitor cells or in fetal cells before hematologic specification suggests that there is a window of opportunity during development for the MLL-AF4 fusion protein to immortalize hematopoietic stem and progenitor cells (HSPCs). (ashpublications.org)
Here, we have determined the growth-inhibitory and proapototic mechanisms of 2 small molecule inhibitors of FLT3, AG1295 or PKC412, in hematopoietic progenitor cells, human leukemic cell lines, and primary AML cells expressing FLT3-ITD. (ashpublications.org)
Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
BC comprises of variant subtypes and those subtypes arise from basal progenitor or luminal progenitor cells undergoing different genetic mutations[ 2 ]. (ijpsonline.com)

120 different translocations, resulting in the generation of MLL-fusion proteins encompassing more than 75 different partner genes 5 . (nature.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
Multidrug resistance caused by the overexpression of ABC transporter proteins in cancer cells remains a major obstacle limiting chemotherapy efficacy. (spandidos-publications.com)
Proadifen also decreased the expression of γH2AX, which is involved in the recruitment of reparation proteins. (spandidos-publications.com)
It was previously reported that proadifen (SKF-525A), a well-known cytochrome P450 monooxygenase inhibitor, not only has anti-proliferative potential in some cancer cell lines, but it is also able to inhibit BCRP and MRP1 transporter proteins ( 8 ). (spandidos-publications.com)
05. Between the 69 ejaculated proteins, 68 may very well be linked to Gene Ontology data. (smadpathway.com)
2nd, we qualitatively examined genes to search for commonality of function amid proteins with high NSAF. (smadpathway.com)
CSCs exhibit certain properties, such as high expression of various anti-apoptotic proteins, expression of ABC pumps, and others, that would allow them to resist chemotherapeutic agents (Li et al. (docksci.com)
Tripartite Motif Containing 13 (TRIM13), a member of TRIM proteins, is deleted in multiple tumor types, especially in B-cell chronic lymphocytic leukemia and multiple myeloma. (cancerindex.org)

FLT3-ITD expression causes malignant transformation and factor-independent growth when expressed in factor-dependent cell lines. (ashpublications.org)
Hypoxia stimulates cancer cells to acquire a more malignant phenotype via activation of hypoxia-inducible factor 1 (HIF-1). (cancerindex.org)
Hematopoietic stem cell transplantation (HSCT) is an important therapeutic option for patients with non-malignant diseases as well as high-risk and refractory hematopoietic malignancies. (haematologica.org)
BCRP expression was detected in several different solid tumours ( 1 - 5 ), as well as in malignant hematopoietic and lymphoid cells ( 6 ). (spandidos-publications.com)

However, only silencing of Bim, or its direct transcriptional activator FOXO3a, abrogated apoptosis efficiently. (ashpublications.org)
The blood microvascular endothelium consists of a heterogeneous population of cells with regionally distinct morphologies and transcriptional signatures in different tissues and organs. (researchgate.net)

In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
What pathways are this gene/protein implicaed in? (cancerindex.org)
Targeted therapies exploit molecular vulnerabilities unique to cancer cells and typically alter cellular signaling pathways to inhibit tumorigenic growth and promote cell death. (biomedcentral.com)
Alternatively, dasatinib may possibly not be capable of mitigating non apoptotic pathways of motor neuron degeneration caused by mutant SOD1, because non apoptotic programmed cell death has also been implicated in motor neuron harm in G93A mice. (smadpathway.com)

By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)

Phase contrast microscopy evidenced some specific morphological modifications indicating apoptosis allied cytotoxicity of piperine like apoptotic bodies, condensed nucleus and membrane blabbing. (ijpsonline.com)
The apoptotic index of tumor cells in the radiation plus β-elemene-treated group was also significantly higher than those in the untreated group and radiation group. (jcimjournal.com)
In our previous study, we demonstrated the anti-proliferative and pro-apoptotic activity of proadifen in the HT-29 cancer cell line ( 9 ). (spandidos-publications.com)
Therefore, our outcomes suggest that dasatinib ameliorates the phenotype of those animals by suppressing apoptotic cell death of motor neurons brought on by mutant SOD1. (smadpathway.com)

Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo, in human promyelocytic leukemic cell lines, and ex vivo in human blood neutrophils. (cdc.gov)
Induction of CYP4F3 by phenol was also observed in differentiated HL-60 cells, in pro-erythroid cell line K562, and ex vivo in human neutrophiles. (cdc.gov)
The findings demonstrated, for the first time, that benzene and metabolites induce CYP4F3 in human blood cells both in vivo and in vitro. (cdc.gov)
The β-elemene can enhance the effects of irradiation on VX2 carcinoma transplanted to kidney in rabbits in vivo by inducing apoptosis of tumor cells. (jcimjournal.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
Cytotoxic And Endocrine-Disrupting Potential Of atrazine, diazinon, endosulfan and mancozeb in adrenocortical steroidogenic cells of rainbow trout in vivo. (innspub.net)

Mechanistic analysis revealed that phenol , a major metabolite of benzene, significantly induced the expression of CYP4F3A at both mRNA and protein levels in cultured promyelocytic leukemia cells (HL-60) similarly to all-trans retinoic acid (ATRA). (cdc.gov)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
As the number of leukemia cells increases, fewer healthy white blood cells, red blood cells and platelets are produced. (nanocraftcbd.com)
RUNX1-positive leukemia cells in preclinical models. (lu.se)

Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
Because mitoxantrone (MTX) is a strong BCRP substrate and is often used in the treatment of leukemia, we investigated the effect of 24 h proadifen pre-treatment on the cytotoxicity of MTX in leukemic cell lines that are sensitive to MTX (HL-60) and MTX-resistant ABCG2-overexpressing subclone (cBCRP). (spandidos-publications.com)

Different volumes of 70% ethanol and water extracts of I'm-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. (nih.gov)
2021). The antiandrogen enzalutamide downregulates TMPRSS2 and reduces cellular entry of SARS-CoV-2 in human lung cells . (essex.ac.uk)
We tested the effects of Tan II A on melanoma A375, MV3, M14, and other human cell lines including Hacat and HUVEC cells in cell culture model. (biomedcentral.com)
and human embryonic stem cells(hESCs). (sigmaaldrich.com)
Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)
Human promyelocytic leukemia cell line HL-60 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and its ABCG2-overexpressing subclone (here referred to as cBCRP) was kindly provided by Dr Balazs Sarkadi (Membrane Research Group, Hungarian Academy of Sciences, Budapest, Hungary) ( 10 ). (spandidos-publications.com)
We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)

Targeting of Mll-AF4 in early fetal HSPCs enhanced the lymphoid potential of primed multipotent cells but was insufficient by itself to induce leukemia. (ashpublications.org)

Induction of apoptosis leads to characteristic cellular changes leading to death. (ijpsonline.com)
Here, we investigated bleomycin-induced lung injury responses in young and aged mice at single-cell resolution to gain insights into the cellular and molecular contributions of aging to fibrosis. (researchgate.net)

TCR stimulation via antigen/MHC presentation or anti-CD3 mAb induces T-ALL cell death. (aacrjournals.org)
possibility is that the damage is not induces mutations in a mutation integrated viral genome ( Todaro and repaired at al , and when the cell rep- assay permits it to be classified as Huebner, 1972) , and alteration of im- licates, the DNA polymerase correct- mutagenic. (who.int)

Further transplantation experiments demonstrated that lympho-myeloid progenitors (LMPPs) allowed the propagation of pMIRH-130b mixed B-cell/myeloid leukemia, whereas Lin-Sca1 +/− Il7 + Kit + blasts maintained pro-B-ALL disease of pMIRH-128a mice. (ashpublications.org)

They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)
Several compounds have been shown to inhibit the efflux activity of ABC transporters, thereby increasing intracellular drug accumulation and sensitising cancer cells to therapy. (spandidos-publications.com)

In addition, cell apoptosis was measured by double staining, DNA fragmentation and annexin V-fluorescein isothiocyanate binding assays. (ijpsonline.com)
Immunoblotting, reporter assays, quantitative RT-PCR, and chromatin immunoprecipitation were performed to evaluate HIF-1 signaling in glioblastoma cells. (cancerindex.org)

Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)
Through detailed characterization of fundamental molecular mechanisms of normal and perturbed cells, the theme strives to unravel information that increases the understanding of various diseases such as cancer and neurodegenerative, cardiovascular and inflammatory disorders. (ki.se)
The stem cell and developmental biology program at CMB is engaged in a broad range of basic research aimed at understanding the molecular mechanisms underlying diverse aspects of embryonic development and tissue regeneration. (ki.se)

Gene landscape and correlation between B-cell infiltration and programmed death ligand 1 expression in lung adenocarcinoma patients from The Cancer Genome Atlas data set. (cancer-genetics.org)

To make the gene signature and risk cutoff widely applicable, we developed a novel analytic approach based on mRNA expression levels of gene-pairs to construct a robust prognostic model, and show how one-hot binary encoding of the gene-pairs effectively eliminates the influence of batch effects. (nature.com)
mRNA microarray analyses of A549, PC14, and PC14CDDP cells that were transiently transfected with either pre-miR-197 or LNA-miR-197 and their controls. (nih.gov)

In 1997, Bonnet & Dick (1997) published their findings concerning the existence of CSCs, by isolating a subpopulation of leukemic cells. (docksci.com)
According to recent findings, especially targeting cancer stem cells (CSCs) is the most promising therapeutic strategy. (bmbreports.org)

PML-RARA represses genes responsible for hematopoiesis and activates super-enhancer genes responsible for specific characteristics of APL. (medscape.com)

Cell migration ability melanoma A375 was monitored by using cell scratch assay. (biomedcentral.com)
Tan II A reduced CXCL12-induced A375 cell invasive ability and migration in a dose dependent manner. (biomedcentral.com)
Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)

in particular, benzene-induced aberrant gene expression has not been addressed. (cdc.gov)
Increased expression of inflammatory cytokines, lysyl oxidase, transforming growth factor-β, impaired megakaryocyte function, and aberrant JAK-STAT signaling have all been implicated in the pathogenesis of bone marrow fibrosis. (haematologica.org)

Therefore, remedies based on the induction of programmed cell death attracted greater attention for cancer treatment [ 8 ]. (ijpsonline.com)

After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)

Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
6 Causes of early death include leukemic transformation, complications arising from progressive bone marrow failure, portal/pulmonary hypertension, infections, thrombosis and bleeding. (haematologica.org)
It occurs when the bone marrow begins to make blasts, cells that have not yet completely matured. (nanocraftcbd.com)
Chronic myeloid leukemia (CML) , also known as chronic myelogenous leukemia, begins in the blood-forming cells of the bone marrow and then, over time, spreads to the blood. (nanocraftcbd.com)
HCL is caused when bone marrow makes too many B cells (lymphocytes), a type of white blood cell that fights infection. (nanocraftcbd.com)
Myelodysplastic syndromes (MDS) are a group of closely related diseases in which the bone marrow produces too few functioning red blood cells (which carry oxygen), white blood cells (which fight infection), or platelets (which prevent or stop bleeding), or any combination of the three. (nanocraftcbd.com)

I am especially interested in the role of mesenchymal stem cells (MSCs) in this process. (essex.ac.uk)
3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
CMB is comprised of more than 30 independent research groups organized in three themes: Cell Biology, Developmental and Stem Cell Biology and Infection and Cancer. (ki.se)
Key areas of investigation are the identification and functional characterization of stem cell niches in developing tissues and adult organs. (ki.se)
Chronic graft- versus -host disease (cGvHD) is a major cause of morbidity after hematopoietic stem cell transplantation (HSCT). (haematologica.org)
Nanomaterials for targeted drug delivery to cancer stem cells. (docksci.com)
Recent developments in cancer biology have identified the existence of a sub-poplulation of cells - cancer stem cells (CSC) that are resistant to most traditional therapies (e.g. chemotherapy and radiotherapy) and have the ability to repair their damaged DNA. (docksci.com)
One of the possible reasons for the failure of the present therapeutic regimens is the existence of a relatively rare cell population known as cancer stem cells (CSCs) that are resistant to or not targeted by present therapeutic modalities. (docksci.com)
For the past 20 years, plenty of studies have suggested that only a small subpopulation of the cancer cells with tumor-initiating capability is the core origin of the tumorigenesis and the subset of cancer cells was named cancer stem cells (CSCs). (bmbreports.org)
As it can be inferred from its nomenclature, CSCs share several features of normal stem cells. (bmbreports.org)

In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)

It is mainly expressed in hematopoietic cells and has an important role in regulating gene expression. (medscape.com)

We also found that viable NK reg cells may be isolated by sorting on CD56 + and CD16 - NK cells, and this population can suppress allogeneic CD4 + T cells, but not T reg cells or CD8+ T cells through a non-cytolytic, cell-cell contact dependent mechanism. (haematologica.org)
With further investigation we may decipher the mechanism of NK reg suppression and operationalize expansion of NK reg cells associated with cGvHD suppression. (haematologica.org)

For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The Ph chromosome is an abnormality that occurs when a piece of chromosome 22 breaks off and attaches to the end of chromosome 9, which also breaks off and attaches to chromosome 22.The breaks in both chromosomes cause the BCR and ABL genes, which combine to create the cancer gene. (nanocraftcbd.com)
The retinoic acid alpha receptor gene ( RARA ) is encoded by the long arm of chromosome 17. (medscape.com)
The promyelocytic gene ( PML ) is encoded by the long arm of chromosome 15 and is expressed ubiquitously. (medscape.com)
In about 5% of cases, rearrangements of chromosome 17q21 with other gene partners occur. (medscape.com)

These blasts normally develop into white blood cells. (nanocraftcbd.com)

Scope includes mutations and abnormal protein expression. (cancerindex.org)

HL-60 cells incubated with various amounts (1, 3, 5, 7.5, and 10 micro l/mL) of the extracts for 1-3 days showed dose-dependent, time-dependent growth suppression and decrease in cell viability. (nih.gov)
In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
We aimed to isolate and define the characteristics of regulatory NK (NK reg ) cells associated with suppression of cGvHD. (haematologica.org)
Moreover, this is the first paper to clearly establish that a CD56 bright CD3 - CD16 - perforin- NK reg population associates with a lack of cGvHD and has several unique characteristics, including the suppression of helper T-cell function in vitro. (haematologica.org)
Various immune-suppressive cell populations appear to associate with suppression of cGvHD, including T reg cells, B reg cells, M2 macrophages, dendritic cells, and CD56 bright natural killer (NK) cells. (haematologica.org)
Moreover, ethanolic extracts of I'm-Yunity-Too exhibited more potent activity compared to its aqueous extracts with regard to suppression of the growth and induction of apoptosis, as assayed by the more pronounced downregulation of phosphorylation of Rb and increased cleavage of poly(ADP‑ribose) polymerase (PARP) from its native 112-kDa form to the inactive 89-kDa product. (spandidos-publications.com)
PML is thought to be involved in apoptosis and tumor suppression. (medscape.com)

B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)

The protein encoded by this gene represents the beta subunit and is a type I membrane protein. (cancer-genetics.org)

To test whether extracts also affected normal cells, similar experiments were also performed using isolated peripheral blood lymphocytes from healthy volunteers, with and without stimulation by the mitogen phytohemagglutinin (PHA). (nih.gov)
Exposure to benzene elicits a spectrum of hematotoxicity ranging from reduction of peripheral blood cell counts to aplastic anemia and leukemia. (cdc.gov)
We analyzed differential gene expression in the peripheral white blood cells (WBC) from 7 female patients diagnosed with occupational benzene poisoning and 7 matched controls. (cdc.gov)

I'm-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle restriction through the G(1)/S checkpoint and the induction of apoptosis. (nih.gov)
As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
Muse flow cytometric analysis was performed for assessing of different cell cycle phases. (ijpsonline.com)

This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)
This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)

Examples DNA repair and apoptosis path- concept is that there is a distinct dif- of DNA damage are DNA adducts ways, which are described in detail ference between DNA damage and (i.e. a molecule bound covalently to by Ciccia and El edge (2010) . (who.int)

To address the role of these miRNAs in transformation, the investigators overexpressed them in lineage-marker-depleted Sca1 + Kit + (LSK) fetal liver cells from targeted Mll-AF4 invertor mice (see figure). (ashpublications.org)
Transplantation of Mll-AF4 + LSK cells virally expressing miR-128a ( pMIRH-128a ) or miR-130b ( pMIRH-130b ) induced a hematologic malignancy in mice with increasing penetrance upon propagation into secondary and tertiary recipients. (ashpublications.org)
pMIRH-130b mice presented with a mixed B-cell/myeloid malignancy, whereas pMIRH-128a mice developed pro-B-cell ALL. (ashpublications.org)
A single attainable explanation for your rather tiny effects of dasatinib on this examine is always that the effective results of this treatment on apoptosis have been minimal in motor neurons and couldn't reverse the bodily dysfunction in the mice, despite the improvement in innervation at NMJs. (smadpathway.com)

The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. (cancerindex.org)

In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)

Breast cancer cells were treated with varying concentrations (1 to 5 mg/ml) of Solanum nigrum extract for 24 and 48 h. (ijpsonline.com)
One of the hallmarks of breast cancer is resistance of tumor cells to cell death. (ijpsonline.com)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)

Transplant experiments in which miRNA knockdown was combined with target overexpression suggest that induction, as well as maintenance, of the disease phenotype is miRNA dependent and involves downregulation of NR2F6 and SGMS1. (ashpublications.org)

Using a robust gene pairing approach, which effectively eliminates batch effects across heterogeneous patient cohorts and transcriptomic data, we developed an immunity and pyroptosis-related prognostic (IPRP) signature that consists of 15 genes. (nature.com)

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. (cancerindex.org)
This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. (cancerindex.org)

Anatomy27

Organisms9

Diseases29

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes56

Disciplines and Occupations1

Information Science3

Health Care1

Proliferation22

Regulation7

Differentiation12

Signaling pathway6

Inhibition8

Tumor cell4

Target genes3

Acute lymphobl3

Autophagy21

Progression6

Progenitor5

Proteins10

Malignant4

Transcriptional2

Pathways4

Promyelocytic HL-601

Apoptotic4

Vivo6

Leukemia cells4

Cytotoxicity2

Human9

Induce1

Cellular2

Induces2

Progenitors1

Inhibit2

Assays2

Arrest2

Mechanisms3

Ligand1

MRNA2

Findings2

Activates1

Migration4

Aberrant2

Programmed cell1

Cytokine1

Bone6

Stem10

EPITHELIAL1

Hematopoietic cells1

Mechanism2

Chromosome5

Blasts1

MUTATIONS1

Suppression7

Tumors1

Membrane1

Peripheral3

Cycle4

Lymphoma2

Colorectal cancer cell1

Distinct1

Mice4

Protein encoded by this gene1

Survival1

Breast cancer4

Phenotype1

Immunity1

Encodes2