• We identified differentially expressed genes from 14 case-control human breast cancer gene expression datasets and integrated them with drug-protein networks. (sutterhealth.org)
  • Differentially expressed genes (DEGs) may be responsible for phenotypic differences between various biological conditions. (mdpi.com)
  • DEGs, or Differentially Expressed Genes, are genes whose expression levels show significant differences between two or more conditions or experimental groups. (pharmiweb.com)
  • After identifying Differentially Expressed Genes (DEGs), we delve deeper into their functional significance. (pharmiweb.com)
  • Hypergeometric analysis was used to identify canonical pathways and curated networks having statistically significant enrichment of differentially expressed genes. (urotoday.com)
  • Cluster analysis identified 3 gene expression signatures on the basis of IRF4 and IRGs expression which were differentially used by SLE and RA patients. (frontiersin.org)
  • The mHTT and wtHTT groups showed the highest number of differentially expressed genes compared to the BACHD mouse model. (lu.se)
  • In BACHD mice, few hypothalamic genes were differentially expressed compared to age-matched WT controls. (lu.se)
  • Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. (wikipedia.org)
  • These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA. (wikipedia.org)
  • Gene expression in living cells is strictly self-regulated to ensure that the correct amounts of proteins are made at the most appropriate timing and location for maintaining cellular homeostasis. (nature.com)
  • Similarly, while your genes are "fixed", the expression of those genes - the amount of proteins they cause to be made, whether or not they are even switched on or off at all - depends on the "environment," the circumstances surrounding those genes . (marksdailyapple.com)
  • Although non-coding RNAs fail to produce functional proteins, researchers believe that in some cases these RNAs may control gene expression. (sciencedaily.com)
  • Cells regulate the expression of their genes with proteins called transcription factors,' said co-corresponding author Dr. Josephine C. Ferreon, assistant professor of pharmacology and chemical biology and member of the Dan L Duncan Comprehensive Cancer Center at Baylor. (news-medical.net)
  • EPFL scientists have just found that members of the DUX family of proteins are responsible for igniting the gene expression program of the nascent embryo. (news-medical.net)
  • The study points to DUX4, and by extension the DUX family of proteins, as the master regulator responsible for kick-starting genome expression at the earliest stage of embryonic life in humans, mouse and probably all placental mammals. (news-medical.net)
  • The fact that the Gimap genes are located together in a tight cluster on RNO4 (and in conserved synteny with many other species), combined with their sequence similarities, suggests the possibility that the proteins carry out similar function. (hindawi.com)
  • Applying the same criterium to the process of gene expression, which also involves many 'intermediate steps', would lead us to the conclusion that no genetic information is transmitted from genes to proteins, which is no less difficult to defend. (cyclingforums.com)
  • In genetics and genomics research, gene expression refers to the process by which information encoded in a gene's DNA sequence is converted into functional proteins or RNA molecules. (pharmiweb.com)
  • Analysis of the pattern of proteins or messenger RNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics.This typically involves the visualization of a few proteins or expressed genes at a time. (lu.se)
  • We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
  • Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)
  • While transcription of prokaryotic protein-coding genes creates messenger RNA (mRNA) that is ready for translation into protein, transcription of eukaryotic genes leaves a primary transcript of RNA (pre-RNA), which first has to undergo a series of modifications to become a mature RNA. (wikipedia.org)
  • These profiles measure mRNA, not protein, so further studies are needed to corroborate that any detected changes in gene expression actually have meaning in the life of the cell. (genengnews.com)
  • Using mRNA from total blood leukocytes, they found that a full one-quarter of the human genome exhibits changes in expression levels during the early stages of post-trauma (40-80 hours post-trauma). (genengnews.com)
  • While researchers once believed that only genes were transcribed into messenger RNA (mRNA), investigators have recently discovered that non-coding DNA is copied into mRNA as well. (sciencedaily.com)
  • Pulmonary OPN relative mRNA expression (peaked at 7 d) and protein levels were induced in wild-type mice following MWCNT exposure. (cdc.gov)
  • Pulmonary cytotoxicity, inflammatory cell influx, and relative mRNA expression of genes related to macrophage function and recruitment (Ccl2, Ccl22, and Arg1) were increased as a result of MWCNT exposure but not affected by OPN deficiency. (cdc.gov)
  • All steps in the gene expression process may be modulated (regulated), including the transcription, RNA splicing, translation, and post-translational modification of a protein. (wikipedia.org)
  • Regulation of gene expression gives control over the timing, location, and amount of a given gene product (protein or ncRNA) present in a cell and can have a profound effect on the cellular structure and function. (wikipedia.org)
  • RNA polymerase II (Pol II) transcribes all protein-coding genes but also some non-coding RNAs (e.g., snRNAs, snoRNAs or long non-coding RNAs). (wikipedia.org)
  • A classic example is the heat shock protein system, particularly Hsp90 , whose expression is environmentally plastic and increases under thermal stress, but buffers phenotypic changes induced by mutations to maintain homeostasis 2 , a process termed canalization 3 . (nature.com)
  • The expression level from luc 25.7k is 1000-times higher than that with luc 1.7k, indicating that the protein yield per target gene is increased by 1000-times of magnitude for a longer template, luc 25.7k (Fig. 1 d). (nature.com)
  • In the comment section of last week's post, Ed was interested in other concrete examples of gene expression (the ability of a gene to produce a biologically active protein). (marksdailyapple.com)
  • While the CD79b gene normally codes for a protein in blood lymphocytes, researchers discovered that CD79b appears to play a very different role in the pituitary gland. (sciencedaily.com)
  • Gene expression is the process by which the information encoded in a gene is used to direct the assembly of a protein molecule. (lupusresearch.org)
  • They found that DUX4 is one of the very first genes expressed at this stage, releasing a high concentration of its protein product just before zygotic genome activation. (news-medical.net)
  • In line with this lead, the scientists could show that the DUX4 protein binds to the regulatory region of genes that are induced during zygotic genome activation, stimulating their expression. (news-medical.net)
  • EHRs provide real-world treatment and outcome patterns, while complementary biomolecular data, including disease-specific gene expression and drug-protein interactions, provide mechanistic understanding. (sutterhealth.org)
  • The first 2 pairs were also enriched among pairs discovered using gene expression data and are supported by molecular interactions in drug-protein networks and preclinical and epidemiologic evidence. (sutterhealth.org)
  • Positional cloning of lymphopenia ( lyp ) in the BB rat revealed a frameshift mutation in Gimap5 , a member of at least seven related GTPase Immune Associated Protein genes located on rat chromosome 4q24. (hindawi.com)
  • Gimap5 is a member of at least seven related GTPase Immune Associated Protein ( Gimap ) genes located within 150 Kilobases (Kb) on rat chromosome (RNO) 4 [ 2 , 3 ]. (hindawi.com)
  • Gimap5 was identified as the lyp gene in the BBDP rat through a frameshift mutation and premature truncation of the Gimap5 protein [ 2 , 6 ] and can be rescued in a P1-derived artificial chromosome (PAC) transgenic rat [ 7 ]. (hindawi.com)
  • Inhibition of albumin gene transcription was also observed upon intravenous infusion of other protein or nonprotein macromolecules, such as gamma-globulin and dextran. (jci.org)
  • Feedback regulation of albumin gene expression by serum colloids may serve as a specific homeostatic mechanism to maintain the steady-state level of total protein in the circulation. (jci.org)
  • Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
  • There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)
  • Research in gene expression is exploding right now and is examining both the impact of environmental factors and the promise of epigenetic therapies. (marksdailyapple.com)
  • SCIENCE TRANSLATIONAL MEDICINE Scientists at Massachusetts General Hospital and Harvard Medical School have-for the first time-imaged epigenetic regulation of gene expression in the living human brain. (the-scientist.com)
  • Similarly, the neural circuits 'translate' the external stimulus, which is 'senseless' to genes, into a specific epigenetic information for their activation/inactivation. (cyclingforums.com)
  • Recent studies support a role for biotin in cell proliferation, DNA repair, epigenetic gene regulation as well as normal immune function. (medscape.com)
  • In addition to environmental factors, phenotypes can also respond to genetic perturbations in a plastic or homeostatic manner, which characterizes the potential of an organism to express phenotypes when genes mutate. (nature.com)
  • Finally, they aim to test the top two gene candidates in mouse models or induced pluripotent stem cells to ensure that they show the predicted expression pattern and to learn whether they exaggerate phenotypes of known autism genes in females but not males. (sfari.org)
  • Many factors can affect gene expression (and thus phenotypes). (msdmanuals.com)
  • Future studies should be clear about methods used to analyze PTSD status, as phenotypes based on PTSD symptom dimensions may yield different gene sets than combined CAPS score analysis. (cdc.gov)
  • The most common approach in transcriptomics (RNA-seq and microarrays) is differential gene expression analysis (DGEA). (mdpi.com)
  • How to Do Differential Gene Expression Analysis? (pharmiweb.com)
  • Differential Gene Expression analysis is a fundamental tool for identifying genes whose expression levels significantly differ between experimental conditions or biological samples. (pharmiweb.com)
  • In genetics, gene expression is the most fundamental level at which the genotype gives rise to the phenotype, i.e. observable trait. (wikipedia.org)
  • Using whole-genome quantitative gene expression as a model, here we study how the genetic architecture of regulatory variation in gene expression changed in a population of fully sequenced inbred Drosophila melanogaster strains when flies developed in different environments (25 °C and 18 °C). We find a substantial fraction of the transcriptome exhibited genotype by environment interaction, implicating environmentally plastic genetic architecture of gene expression. (nature.com)
  • Finally, although genotype by environment interaction in gene expression could potentially disrupt genetic networks, the co-expression networks are highly conserved across environments. (nature.com)
  • Ruderfer DM, Roberts DC, Schreiber SL, Perlstein EO, Kruglyak L. Using expression and genotype to predict drug response in yeast. (broadinstitute.org)
  • Cell-free gene expression systems have been valuable tools for understanding how transcription/translation can be regulated in living cells. (nature.com)
  • PAR-2 and ERGIC-53 genes in HaCaT cells were up-regulated by 3.5 mW/cm2 millimeter wave exposure for 4 times. (nih.gov)
  • Gene expression in groups of ear cells after noise damage. (newswise.com)
  • After making their study known to other researchers in their field, the team heard from scientists at Decibel Therapeutics (led by Joe Burns, PhD) and the Karolinska Institute (led by Barbara Canlon, PhD), who had the gene expression data from the inner ear's neurons, side wall and immune cells from before and after noise damage. (newswise.com)
  • In another example, the researchers found that only one out of the four types of immune cells detected showed major differences in gene expression. (newswise.com)
  • De Iaco also found that when DUX4 is forcibly produced in muscle cells, it turns on a whole set of genes that are expressed during zygotic genome activation. (news-medical.net)
  • Next, they looked at mouse embryonic stem cells, which contain the mouse version of the DUX4 gene (called simply DUX). (news-medical.net)
  • When in culture, a small fraction of these cells exhibit at any given time the gene expression pattern of 2-cell stage embryos, before cycling back to the features of more advanced embryonic cells. (news-medical.net)
  • After many years of concerted effort to systematically analyze the expression of genes in all human cells and tissues, RIKEN and the FANTOM consortium publish the findings today in two landmark Nature reports, and 16 related articles in ten other scholarly journals (ref.1,2,3). (riken.jp)
  • Using a highly sensitive technique called Cap Analysis of Gene Expression (CAGE), developed at RIKEN (ref. 4, 5), the researchers monitored the activity of promoters and enhancers across over 180 human primary cells. (riken.jp)
  • The difference between all these cells is what parts of the genome they use - for instance, brain cells use different genes than liver cells, and therefore they work very differently. (riken.jp)
  • Unlike other large-scale genomics projects, FANTOM5 focused on identifying gene expression on normal primary cells rather than cell lines derived from cancers. (riken.jp)
  • Light entering the eye causes genes to be expressed in specific cells. (cyclingforums.com)
  • Synaptic transmitter binding to receptors on melatonin secreting cells in the pineal activate genes in the cell nucleus, but that involves a whole series of intermediate steps. (cyclingforums.com)
  • Now researchers from North Carolina State University have found a way to use naturally occurring hydrogen peroxide inside cells to switch on gene expression. (phys.org)
  • They designed a molecule that was sensitive to hydrogen peroxide and enabled expression of the firefly luciferase gene in live mammalian cells. (phys.org)
  • The exciting aspects of this synthetic gene switch are that you can use it not only as a way to detect the presence of hydrogen peroxide within cells, in this case, by making cells containing hydrogen peroxide glow, but also to express any gene of interest in response to the presence of hydrogen peroxide," Deiters says. (phys.org)
  • Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives" Jeane M. Govan, Andrew L. McIver, Chad Riggsbee and Alexander Deiters, North Carolina State University, Angewandte Chemie , 2012. (phys.org)
  • When studying gene expression, researchers often compare gene expression profiles between different biological samples or experimental conditions, such as healthy and diseased tissues, treated and untreated cells, or different developmental stages. (pharmiweb.com)
  • The locus of the psl polysacchide genes encode the cells to adhere to a surface and thus maintaining the biofilm structure. (ukessays.com)
  • Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
  • We used the paradigmatic GATA-PU.1 axis to explore, at the systems level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. (lu.se)
  • To identify differing patterns of gene expression, they used RNA sequencing (RNAseq), a technique that reflects how strongly genes are being expressed in a given tissue. (eurekalert.org)
  • With RNA-seq , we can capture and quantify the abundance of transcripts, revealing the dynamic landscape of gene expression within a cell or tissue. (pharmiweb.com)
  • By comparing gene expression patterns during embryogenesis or tissue differentiation, we unravel the molecular events that shape organisms, advancing our understanding of developmental biology. (pharmiweb.com)
  • Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics. (lu.se)
  • With this study, we've seen thousands and thousands of genes change how they are turned on and turned off. (upi.com)
  • By analyzing the expression levels of thousands of genes simultaneously, researchers can identify genes that are upregulated (higher expression) or downregulated (lower expression) in one condition compared to another. (pharmiweb.com)
  • These findings will help in the identification of genes involved in disease and the development of personalized and regenerative medicine. (riken.jp)
  • DGE analysis facilitates the identification of genes responsive to specific drug treatments. (pharmiweb.com)
  • But even so, gene-expression profiling can be enormously valuable in finding biomarkers, highlighting druggable targets, revealing the metabolic pathways important in a biological process, and identifying subsets of a population that might respond to a certain therapy or be more susceptible to a certain clinical outcome. (genengnews.com)
  • It is not the external stimulus per se , but the chemical output (=information) generated by processing of the stimulusin the neural circuit that via the respective signal transduction pathways reaches the genes responsible for the synthesis of melatonin, which is intelligible to (i.e. contains informatio for) activating those genes. (cyclingforums.com)
  • By subjecting DEGs to gene ontology enrichment analysis, pathway analysis, or functional annotation, we gain insights into the biological processes, molecular functions, and pathways associated with the observed gene expression changes. (pharmiweb.com)
  • Characterisation of gene expression patterns in bladder cancer allows the identification of pathways involved in its pathogenesis, and may stimulate the development of novel therapies targeting these pathways. (urotoday.com)
  • In AAV groups, from the combined limma with GSEA using ClusterProfiler, we found both shared and unique gene sets and pathways for mice with wtHTT overexpression compared to mice with mHTT overexpression. (lu.se)
  • Multiple biosynthetic pathways such as sterol synthesis were among the top shared processes, where both unique and shared genes constituted leading-edge subsets. (lu.se)
  • Gene-expression tests like this, that analyze a small number of genes at a time, are widely available for breast, prostate and some other cancers, and they've proven to be a very accurate and inexpensive alternative to other types of tests," said Chen. (ucsf.edu)
  • This chapter describes methods to analyze gene expression in S. aureus during interaction with human neutrophils . (bvsalud.org)
  • To analyze microarray datasets (34760 variables) and obtain functional implications of differential expression patterns, we used Linear Models for Microarray Data (limma) followed by Gene Set Enrichment Analysis (GSEA) using ClusterProfiler. (lu.se)
  • Here we report the effect of the length of linearized reporter DNAs encoding the firefly luciferase gene so as to exclude the influence of supercoiling. (nature.com)
  • This alignment process enables the determination of the origin of each read, allowing us to associate it with specific genes or genomic regions. (pharmiweb.com)
  • For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
  • In other words, expression of specific genes might have relevance to pathogenesis of diseases. (intechopen.com)
  • Researchers around the globe are harnessing gene-expression profiling studies in a variety of clinical settings to prevent and treat a number of diseases. (genengnews.com)
  • Researchers recently discovered a novel mechanism that works over an extensive genomic distance and controls the expression of human growth hormone (hGH) in the pituitary gland. (sciencedaily.com)
  • Researchers at the University of Pennsylvania School of Medicine recently discovered a novel mechanism that works over an extensive genomic distance and controls the expression of human growth hormone (hGH) in the pituitary gland. (sciencedaily.com)
  • By examining the relationship between these non-coding RNAs and the hGH gene, researchers hope to understand how these remote regions impact hGH gene expression and dysfunction. (sciencedaily.com)
  • While mutations to the hGH gene often lead to abnormal growth in children and adults, these mutations have provided researchers with key clues regarding the genomic areas that appear to control expression of the hGH gene. (sciencedaily.com)
  • A team led by researchers at Baylor College of Medicine has discovered a mechanism by which transcription factor KLF4 can help to organize chromatin, thus influencing gene expression. (news-medical.net)
  • The team added their newest data on noise-induced hearing loss to gEAR - Gene Expression Analysis Resource - a tool developed by her laboratory that allows researchers not trained in informatics to browse gene expression data ( published earlier this summer ). (newswise.com)
  • But when the EPFL researchers deleted the DUX gene, this process stopped, the appearance of the 2-cell stage-like subpopulation was suppressed. (news-medical.net)
  • Now, researchers at UC San Francisco and Northwestern Medicine, in collaboration with 10 other medical centers, have found a highly accurate way to predict the best treatment for patients based on patterns of gene expression - which genes are turned on and off - in their tumors. (ucsf.edu)
  • By looking for brain regions and stages of development at which these genes converge, the researchers will seek to understand when, where and how the female protective effect occurs. (sfari.org)
  • In this genomic age, one possible answer is that a cell can be defined by a profile of all of the genes it is expressing. (genengnews.com)
  • At the genomic level, a 14,000 base-pair separation is equal to the size of 10 growth hormone genes lined end to end. (sciencedaily.com)
  • Genomic imprinting is the differential expression of genetic material depending on whether it has been inherited from the father or mother. (msdmanuals.com)
  • Gene expression is summarized in the central dogma of molecular biology first formulated by Francis Crick in 1958, further developed in his 1970 article, and expanded by the subsequent discoveries of reverse transcription and RNA replication. (wikipedia.org)
  • Hara Levy, professor of physiology at the Human and Molecular Genetics Center of the Medical College of Wisconsin, studies cystic fibrosis (CF). Although CF is caused by mutations in the CF transmembrane conductance regulator gene, patients with identical genetic mutations in this gene can suffer variable levels of lung disease severity. (genengnews.com)
  • Expressing a particular gene requires that the gene expression molecular machinery has access to that stretch of DNA. (news-medical.net)
  • The purpose of this study was to develop a robust, standardized method for comparative expression profiling and molecular characterization of Leishmania donovani clinical isolates. (cambridge.org)
  • Intracellular amastigote protocols need further refinement, but we describe a first generation tool for high-throughput comparative molecular characterization of patients' isolates, based on the changing expression profiles of promastigotes during in vitro differentiation. (cambridge.org)
  • The molecular basis for this proposed regulation has now been investigated by adding oncotically active macromolecules to the circulation of normal or genetically albumin-deficient Nagase analbuminemic rats (NAR) and analyzing the hepatic expression of genes, including albumin after 24 h. (jci.org)
  • Stephan Sanders and his colleagues have previously shown that spontaneous, or de novo , mutations (seen only in the child and not in either parent) are important risk factors for autism and can be used to find the genes involved in causing autism. (sfari.org)
  • To find these mutations, they plan to build a map of the differences in gene expression between males and females in multiple brain regions and across multiple stages of brain development. (sfari.org)
  • They hope to look for a group of genes that have mutations in girls with autism and that also show a difference in brain expression between the sexes. (sfari.org)
  • E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
  • Intravenous infusion of albumin into normal rats also decreased transcriptional activity of the albumin gene by 50-60%, and this decrease correlated with changes in serum colloid osmotic pressure after albumin infusion. (jci.org)
  • In this study, we investigated whether hypothalamic HTT expression causes transcriptional changes. (lu.se)
  • Regulation of gene expression is the basis for cellular differentiation, development, morphogenesis and the versatility and adaptability of any organism. (wikipedia.org)
  • An alternative approach is gene coexpression analysis, which detects groups of genes with similar expression patterns across unrelated sets of transcriptomic data from the same organism. (mdpi.com)
  • These changes in RNA levels are known as changes in "gene expression. (newswise.com)
  • She collected data on the changes in gene expression from before and after noise damage. (newswise.com)
  • The differential expression of IRF4 in RA was then confirmed in publicly available gene expression datasets. (frontiersin.org)
  • Many studies have investigated the determining factors that affect gene expression. (nature.com)
  • Millimeter wave exposure could affect gene expression in human keratinocytes, which might be related to the intensity and the times of exposure. (nih.gov)
  • Recent global analyses of gene transcripts revealed that specific transcription factors (TFs) and their networking systems physiologically correspond to the onset of human diseases, including cancer. (intechopen.com)
  • Our more granular analyses of PTSD symptom dimensions identified additional genes that did not reach statistical significance in our analyses with total CAPS scores. (cdc.gov)
  • The human genome is comprised of both non-coding DNA and coding regions, or genes. (sciencedaily.com)
  • Gene expression profiles were obtained using the Affymetrix human genome U95A GeneChip. (nih.gov)
  • The papers published in Nature describe maps of promoters and enhancers - short regions of DNA that influence the activity of genes - encoded in the human genome, and their activity across the vast wealth of human cell types and tissues of the human body. (riken.jp)
  • Microarray technology and next-generation sequencing methods have enabled us to see all of the genes expressed by a given cell type at any particular time, or under a particular circumstance. (genengnews.com)
  • The early findings suggest space travel boosts methylation, the process of switching genes on and off. (upi.com)
  • Formation of this biomolecular condensate is strongly enhanced by a DNA modification called CpG methylation, a change that influences gene expression,' said co-corresponding author Dr. Kevin MacKenzie, associate professor of pathology and immunology and of pharmacology and chemical biology at Baylor. (news-medical.net)
  • We identified 66 genes significantly associated with total highest lifetime CAPS score (FDR-corrected p (cdc.gov)
  • In particular, we identified 82 genes significantly associated with lifetime anxious arousal symptoms. (cdc.gov)
  • Several genes significantly associated with multiple PTSD symptom dimensions and total lifetime CAPS score (SERPINA1, RPS6KA1, and STAT3) have been previously associated with PTSD. (cdc.gov)
  • In addition, we enable manipulation of the cellular gene expression program by providing different genome-wide collections of ORFs. (epfl.ch)
  • It is expected that the enhancement of gene expression efficiency with a shrunken DNA conformation would also be a rather general mechanism in living cellular environments. (nature.com)
  • To elucidate the complex mechanism of gene regulation in living cellular systems, it is necessary to closely investigate each phase of gene expression and also to shed light on the cooperative effects between transcription and translation. (nature.com)
  • Small and Large Ribosomal Subunit Deficiencies Lead to Distinct Gene Expression Signatures that Reflect Cellular Growth Rate. (broadinstitute.org)
  • Although the majority of expression quantitative trait loci (eQTLs) for the gene expression traits in the two environments are shared and have similar effects, analysis of the environment-specific eQTLs reveals enrichment of binding sites for two transcription factors. (nature.com)
  • Lastly, we determined whether synergistic drug pairs found in the EHRs were enriched among synergistic drug pairs from gene-expression data using a method similar to gene set enrichment analysis. (sutterhealth.org)
  • Gene Set Enrichment Analysis (GSEA) with leading-edge analysis showed that suppressed sterol- and cholesterol metabolism were shared between hypothalamic wtHTT and mHTT overexpression. (lu.se)
  • This study involves a comprehensive, multi-modal, and integrative assessment of biomarkers implicated in the pathophysiology of PTSD, including measuring differences in whole-blood gene expression and other blood biomarkers of key neurobiological systems, an approach critical to informing risk and resilience prediction algorithms for PTSD, and to develop novel psychopharmacologic approaches for the treatment of this disabling condition in disaster responders and other trauma survivors. (cdc.gov)
  • Analysis of gene expression from these individuals can help in identifying biomarkers of PTSD. (cdc.gov)
  • To test whether the transcription of the locus control region and CD79b played a significant role in hGH expression in transgenic mice, Ho then inserted a special piece of DNA into the locus control region. (sciencedaily.com)
  • BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)
  • RNA polymerase III transcribes 5S rRNA, transfer RNA (tRNA) genes, and some small non-coding RNAs (e.g., 7SK). (wikipedia.org)
  • This mechanism involves a newly discovered set of "non-coding RNAs" expressed in the vicinity of the hGH gene. (sciencedaily.com)
  • When you change to a diet low in sugars and rich in healthy fats, those or other genes are directed to reduce inflammatory expression, down-regulate insulin-producing metabolic machinery, up-regulate insulin receptors and rebuild cell membranes to reflect the presence of better building materials (omega 3 fatty acids, etc. (marksdailyapple.com)
  • FANTOM, a large international consortium led by RIKEN releases today the first comprehensive map of gene activity across the human body, and provides the first holistic view of the complex networks that regulate gene expression across the wide variety of cell types that make up a human being. (riken.jp)
  • A key discovery on the way was that by employing CAGE, the technology used to find active genes, the team could identify the additional DNA regions that regulate the activity of genes in every cell type, called enhancers. (riken.jp)
  • Using a genetically modified mouse model, Nancy E. Cooke, MD, Stephen A. Liebhaber, MD, Professors of Genetics and Medicine, and colleagues, demonstrated a critical role of two non-coding regions on the activation of the hGH gene. (sciencedaily.com)
  • By carefully analyzing the 14,000 base pairs separating the hGH gene and its locus control region, co-authors Yugong Ho, PhD, an Instructor of Genetics at Penn and a Cooke/Liebhaber lab member, and Felice Elefant, PhD, Assistant Professor at Drexel University and former member of the Cooke/Liebhaber lab, found that the locus control region was copied into RNA, and discovered a gene called CD79b within this region. (sciencedaily.com)
  • Because TNF-alpha expression is upregulated in hepatic inflammation, the data suggest TNF-alpha-mediated increases in tTG expression may play an important role in the process of hepatic fibrogenesis. (nih.gov)
  • We are pleased to announce the twelfth Cold Spring Harbor meeting on Gene Expression & Signaling in the Immune System , which will begin on Tuesday evening, April 16, and end at noon on Saturday, April 20, 2024. (cshl.edu)
  • We are looking forward to a broad-based meeting, and abstracts are welcomed on all scientific topics related to immune system signaling, gene regulation, and effector activities. (cshl.edu)
  • Nucleus-specific expression in the multinuclear mushroom-forming fungus reveals different nuclear regulatory programs. (broadinstitute.org)
  • A novel feedback regulatory mechanism operating on transcription of the albumin gene is described in the rat. (jci.org)
  • Impaired control of Interferon-Responding Genes (IRGs) expression by their regulatory mechanisms, including Interferon Regulatory Factors (IRFs), may underlie these findings and it may explain the heterogeneity observed among these conditions. (frontiersin.org)
  • and (4) how dynamic modeling of DNA-binding and gene expression data can be used to infer regulatory logic of TF circuitry. (lu.se)
  • In the current study, we focused on master transcription factor KLF4, which is known to selectively mediate gene expression and reprogramming that determines cell fate. (news-medical.net)
  • Dr. Hertzano says it was so important that they looked at a cell specific level, rather than looking at the entire ear because they found that most of the gene expression changes were specific to only one or two cell types. (newswise.com)
  • The cell reads the sequence of the gene in groups of three bases. (lupusresearch.org)
  • In FANTOM5, we have for the first time systematically investigated exactly what genes are used in virtually all cell types across the human body, and the regions which determine where the genes are read from the genome," explains Dr. Alistair Forrest, scientific coordinator of FANTOM5. (riken.jp)
  • At this time, there is a paucity of information as to the expression of the Gimap genes in specific cell types. (hindawi.com)
  • A very similar set of genes is induced in unlesioned animals by administration of the psychostimulant cocaine or the antipsychotic eticlopride, although in distinct striatal cell populations. (jneurosci.org)
  • When hydrogen peroxide was present, the luciferase gene was expressed, causing the cell to glow. (phys.org)
  • When KLF4 interacts with specific chromatin regions, it forms a condensate - a separate liquid phase - that preferentially recruits other molecules that help open the chromatin and mediate gene transcription. (news-medical.net)
  • Transcription factors such as KLF4 are involved in reorganizing the chromatin to provide access and facilitate gene transcription, but it is not clear how this is accomplished. (news-medical.net)
  • Type I IFN production is tightly controlled at the gene expression level in a highly ordered process regulated by multiple transcription factors ( 14 ). (frontiersin.org)
  • This analysis enables us to pinpoint genes that play pivotal roles in phenotypic variation, disease development, or response to treatments. (pharmiweb.com)
  • And we can influence gene expression to a far greater degree than anyone ever thought possible. (marksdailyapple.com)
  • Diet, exercise, exposure to toxic chemicals (or fresh air), medicines, even the thoughts you think (which generate actual chemical signals) all influence gene expression - positively and/or negatively, depending on the choice. (marksdailyapple.com)
  • The study, published in the journal Nature Communications, reveals that the binding of KLF4 can cause DNA to condense into a separate liquid phase in a process called biomolecular condensation, which recruits other factors that influence gene expression. (news-medical.net)
  • Genetic variance in expression increases at 18 °C relative to 25 °C for most genes that have a change in genetic variance. (nature.com)
  • Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR. lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background. (hindawi.com)
  • Keeping the boron out of the ER: A genetic switch was engineered that activates gene expression in the presence of H2O2. (phys.org)
  • Analysis of Staphylococcus aureus gene expression during PMN phagocytosis. (bvsalud.org)
  • His innovation was to correlate the within-patient change in gene expression with the severity of the clinical outcome, rather than using absolute expression values as has traditionally been done. (genengnews.com)
  • On the contrary, we found that the subset of neurons that are resistant to noise trauma, turn on a program that protects them while the very sensitive neurons had little change in gene expression. (newswise.com)
  • The process of gene expression is used by all known life-eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses-to generate the macromolecular machinery for life. (wikipedia.org)
  • It took me a long time to appreciate just how extensively population heterogeneity and other unmeasured sources of variation introduce unwanted and systematic biases into the statistical analysis of gene-expression studies," Dr. Storey noted. (genengnews.com)
  • The FANTOM project (for Functional Annotation of the Mammalian genome) is a RIKEN initiative launched in 2000 to build a complete library of human genes using the capabilities offered by new, state-of-the-art cDNA technologies. (riken.jp)
  • At the "Critical Assessment of Massive Data Analysis" conference held recently in Vienna, John Storey, from the Lewis-Sigler Institute for Integrative Genomics at Princeton University, gave a talk on dissecting the inflammatory complications in critically injured patients by within-patient gene-expression changes. (genengnews.com)
  • Eat a diet that is high in sugar, and gene expression moves in a direction that produces more insulin, that shuts off insulin receptors, that down-regulates lipase and other enzymes involved in fat-burning, that increases pro-inflammatory cytokines, etc. (marksdailyapple.com)
  • Although these factors have been studied extensively, little is known about how the length of the DNA template influences gene expression. (nature.com)
  • Using samples from 1,856 meningioma patients at 12 medical centers in the U.S., Europe and Hong Kong, Raleigh's team came up with a set of 34 genes whose gene expression patterns had the potential to predict whether a tumor would return. (ucsf.edu)
  • Citations to Albumin gene expression is down-regulated by albumin or macromolecule infusion in the rat. (jci.org)
  • Albumin gene expression is down-regulated by albumin or macromolecule infusion in the rat. (jci.org)
  • The transcription rate of the albumin gene was higher in NAR than in normal rats and was dramatically reduced by raising serum albumin to 1.6 g/dl. (jci.org)
  • 1 % of alleles, expression is possible only from the paternal or maternal allele. (msdmanuals.com)
  • The present study contributes to a growing body of work on gene expression signatures in bladder cancer. (urotoday.com)
  • The differential expression of IRF4 and IRGs observed in SLE and RA can delineate gene expression signatures associated with clinical features and treatment outcomes. (frontiersin.org)
  • We expected the subset of neurons typically sensitive to noise and aging, to have "bad" changes in genes, so that we could counter them with drugs, but there was no such thing," said Dr. Hertzano. (newswise.com)
  • Once the reads are mapped, we quantify the expression level of each gene. (pharmiweb.com)
  • In this research project we try to quantify the gene expression of the above genes pilB, pslA, lecA, ureB using rpoD as the house keeping gene. (ukessays.com)
  • Previous work in the laboratories of Cooke and Liebhaber found that the hGH gene is controlled by a non-coding DNA region, or locus control region. (sciencedaily.com)
  • The effects of the locus control region on human growth hormone expression is as if you turn a key in the lock of a house at one end of your street and find that this action opens the lock and door of a house a block away," notes Liebhaber. (sciencedaily.com)
  • RNAseq of the dorsolateral prefrontal cortex and the nucleus accumbens, two brain areas strongly associated with addiction pathophysiology, showed that certain genes were expressed differently in people with OUD compared to controls. (eurekalert.org)
  • We combined global ChIP-seq of GATA1, GATA2, and PU.1 with expression profiling during differentiation to erythroid and neutrophil line- ages. (lu.se)
  • Coexpressed genes tend to be involved in similar biological processes. (mdpi.com)
  • The limitations and possibilities associated with expression profiling in intracellular amastigotes and promastigotes were assessed through a series of comparative experiments in which technical and biological parameters were scrutinized. (cambridge.org)
  • On the biological level, we demonstrate that gene expression levels fluctuate during in vitro development of both intracellular amastigotes and promastigotes. (cambridge.org)
  • We chose to use expression-curves rather than single, specific, time-point measurements to capture this biological variation. (cambridge.org)
  • The distinction in significant genes between total lifetime CAPS score and the anxious arousal symptom dimension of PTSD highlights a potential biological difference in the mechanism underlying the heterogeneity of the PTSD phenotype. (cdc.gov)
  • his statistical challenge in this study was to correlate gene expression over multiple time points, in multiple patients, with multiple and different clinical presentations. (genengnews.com)
  • In a paper appearing Nov. 9, 2023 in Nature Medicine , the team concluded that just 1 in 5 patients with low-grade tumors (those less likely to regrow) may need radiation, while around 2 in 5 with higher-grade tumors may be better off without radiation, based on the results of the new gene-expression test. (ucsf.edu)
  • Raleigh and Chen and their multidisciplinary team suspected that gene expression could more accurately point out the patients who would be helped by radiotherapy. (ucsf.edu)
  • Expression of survivin messenger RNA was evaluated by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in patients with MDS and acute myeloid leukemia (AML). (spandidos-publications.com)
  • Eleven out of 12 patients with refractory anemia (RA) (91.6%), and all 3 patients with refractory anemia with excess blasts in transformation (RAEBt) (100%), were positive for survivin expression with the majority of cases showing abundant levels of the survivin transcript. (spandidos-publications.com)
  • On the other hand, expression of survivin was undetectable in the 4 patients with chronic myelomonocytic leukemia (CMMoL). (spandidos-publications.com)
  • The level and frequency of survivin expression in patients with refractory anemia were compared to those in patients with AML. (spandidos-publications.com)
  • Out of 12 patients with de novo AML, 5 patients (41.7%) showed detectable levels of survivin expression. (spandidos-publications.com)
  • In the present study we aimed to evaluate the associations between IRF4 gene expression and those of IRGs in SLE and RA patients to gain insight about its links with the IFN signature as well as to explore the potential clinical relevance of these associations. (frontiersin.org)
  • The gene expression of IRF4 and IRGs (IFI44, IFI44L, IFI6, and MX1) in peripheral blood was analyzed in 75 SLE patients, 98 RA patients, and 28 healthy controls. (frontiersin.org)
  • Patients with minimal expression are sometimes considered to have a forme fruste of the disorder. (msdmanuals.com)
  • CONCLUSIONS: This is a proof-of-concept study demonstrating that a combination of complementary data sources, such as EHRs and gene expression, can corroborate discoveries and provide mechanistic insight into drug synergism for repurposing. (sutterhealth.org)
  • Our data substantiates the Gimap5 frameshift mutation as the primary defect with only limited contributions to lymphopenia from the remaining Gimap genes. (hindawi.com)
  • Structural changes and neuropathology in the hypothalamus have been suggested to contribute to the non-motor manifestations of Huntington's disease (HD), a neurodegenerative disorder caused by an expanded cytosine-adenine-guanine (CAG) repeat in the huntingtin (HTT) gene. (lu.se)
  • In contrast, the ubiquitous expression of full-length mHTT in the BACHD hypothalamus moderately affects the transcriptomic profile. (lu.se)
  • The final piece of evidence came when the EPFL scientists removed the DUX gene from fertilized mouse oocytes using CRISPR/Cas9 genome editing. (news-medical.net)