Gene encoding bacteriophage t7 rna polymerase. Medical search. Frequent questions

a plastid-encoded bacterial-type RNA polymerase (PEP) and a nuclear-encoded phage-type RNA polymerase (NEP), which recognize distinct types of promoters. (frontiersin.org)
KP1345 was built by transduction of GUC41 with Sobre3, which encodes T7 RNA polymerase, and connected phage lysates, as referred to in the Sobre3 lysogenization package. (informationalwebs.com)
A search of human gut metagenomes for circular contigs encoding phage hallmark genes resulted in the identification of 3738 apparently complete phage genomes that represent 451 putative genera. (biomedcentral.com)
Some "Quimbyviridae" phages possess Diversity-Generating Retroelements (DGRs) that generate hypervariable target genes nested within defense-related genes, whereas the previously known targets of phage-encoded DGRs are structural genes. (biomedcentral.com)
The replication of the genomic DNA occurs concurrently with phage gene expression, protein synthesis, and viral assembly. (arborbiosci.com)
Phage ϕIBB-PF7A is a T7-like bacteriophage capable of infecting several Pseudomonas fluorescens dairy isolates and is extremely efficient in lysing this bacterium even when growing in biofilms attached to surfaces. (biomedcentral.com)
Twenty eight (46.7%) of the proteins encoded by this virus exhibit sequence similarity to coliphage T7 proteins while 34 (81.0%) are similar to proteins of Pseudomonas phage gh-1. (biomedcentral.com)
That this phage is closely related to Pseudomonas putida phage gh-1 and coliphage T7 places it in the "T7-like viruses" genus of the subfamily Autographivirinae within the family Podoviridae . (biomedcentral.com)
Formerly, the phylogenetic and taxonomic classification of bacteriophages (phages) was based on similarities in phage morphology, host range [ 1 ], ability to recombine, and also on similar cross-hybridization patterns of their DNAs [ 2 - 4 ]. (biomedcentral.com)
Today, phage genome sequencing is commonly performed for classification and characterization purposes and is based on the arrangement of conserved genes and the nucleotide and protein sequence identity [ 5 , 6 ]. (biomedcentral.com)
The size of this phage correlates well with other T7-like phage members, where the smallest and largest phages reported so far are the Pseudomonas putida phage gh-1 (37.4 kb) and the Vibrio parahaemolyticus phage VpV262 (45.9 kb), respectively [ 12 ]. (biomedcentral.com)
The new system, called phage-assisted continuous evolution, or PACE, is based on the continuous culture and selection of the M13 filamentous bacteriophage commonly used in phage display. (genengnews.com)
PACE essentially comprises a system by which E. coli cells continuously flow through a fixed-volume vessel, or lagoon, which contains a replicating population of phage DNA vectors (the selection phage) encoding the gene or genes of interest. (genengnews.com)
The system achieves continuous selection by linking the desired activity to the production of infectious progeny phage containing the evolving gene(s). (genengnews.com)
RESULTS: We expressed the gene encoding Enterococcus faecium phage IME199 DNA polymerase (IME199 DNAP) in Escherichia coli BL21(DE3), and characterized protein function. (bvsalud.org)
The mechanism used by the short-tailed phage T7 for crossing the host's envelope layers is to extend the length of its tail upon infection. (asmblog.org)
P2 provides an attractive alternative to phage T4 for detailed molecular studies of tail assembly, because fewer genes are involved. (tam-receptor.com)
The MS2 phage RNA genome comprises a single plus-sense strand encoding 3,569 nucleotides. (politicalespionage.com)

A notable example is the replacement of an original multi-subunit bacteria-like RNA polymerase (inherited from the proto-mitochondrial ancestor and still encoded in certain jakobid - but no other - mitochondrial genomes) by a single-subunit bacteriophage T3/T7-like RNA polymerase, which directs mitochondrial transcription in virtually all eukaryotes [ 24 ]. (biomedcentral.com)
533 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. (novoprolabs.com)
We are investigating several helicases that function in DNA and RNA replication and in transcription termination. (rutgers.edu)
A class of helicases assembles into hexamer rings including T7 DNA helicase and the Rho transcription termination factor. (rutgers.edu)
The viral RNA-dependent RNA polymerase starts transcription in the 3′ end from the genomic RNA inside a sequential way with a stop-start system [5]. (ecologicalsgardens.com)
3 Building of NDV-Vectored Vaccines Infectious NDV could be retrieved completely from cloned cDNA by transfecting cultured cells with plasmids encoding the viral the different parts of an operating nucleocapsid full-length antigenomic RNA as well as the main proteins involved with replication and transcription i.e. the N P and L proteins beneath the control of bacteriophage T7 RNA polymerase promoter [5] (Shape 2). (ecologicalsgardens.com)
This technique which can be known as invert genetics technique is currently designed for all three pathotypes of NDV strains [9 10 11 12 Generally a international gene flanked by NDV GS and GE sequences can be inserted right into a 3′ non-coding area of the NDV genome as yet another transcription unit. (ecologicalsgardens.com)
Because of a polar gradient transcription international genes are indicated better when positioned nearer to 3′ end from the genome. (ecologicalsgardens.com)
Genomic organization of the tag7gene and its promoter region remind those of the genes of the tumornecrosis factor locus, although the tag7 gene is not linked to this locus.The gene is located on chromosome 7 at the area that corresponds to band7A3, which has genetic linkage with lupus-like disease in mouse models.tag7 transcription is essential for lymphoid organs. (embl-heidelberg.de)
Using a cell-free transcription-translation system as a molecular programming platform, we show that the bacteriophage T7, encoded by a 40 kbp DNA program composed of about 60 genes, can be entirely synthesized from its genomic DNA in a test tube reaction. (arborbiosci.com)
Engineering synthetic gene circuits in a test tube, however, is seriously limited by the transcription repertoire of modern cell-free systems, composed of only a few bacteriophage regulatory elements. (arborbiosci.com)
Enzymatic ligation for circular RNA synthesis is typically achieved through in vitro transcription (IVT) reactions. (techadda2.com)
The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes. (wakehealth.edu)
Peginterferon alfa-2a binds to cell surface receptors in a cascade of protein interactions, resulting in gene transcription. (medscape.com)
Transcription is initiated when an inducer binds to the lac repressor to prevent it from inhibiting the E. coli expression of the T7 gene. (pharmaceutical-networking.com)
The genetic information of life on Earth have been conserved in natural nucleotides-A, G, C, T(U) forming two exclusive sets of base pairs A-T(U) & G-C, that complementing to the fundamental processes of DNA replication & RNA transcription by several polymerases and translation to functional proteins via ribosomal reactions. (fortuneonline.org)

A multilevel framework for taxonomic classification of viruses was recently adopted, facilitating the classification of phages into evolutionary informative taxonomic units based on hallmark genes. (biomedcentral.com)
Several "Flandersviridae" phages encode enzymes of the isoprenoid pathway, a lipid biosynthesis pathway that so far has not been known to be manipulated by phages. (biomedcentral.com)
The "Gratiaviridae" phages encode a HipA-family protein kinase and glycosyltransferase, suggesting these phages modify the host cell wall, preventing superinfection by other phages. (biomedcentral.com)
Hundreds of phages in these three and other families are shown to encode catalases and iron-sequestering enzymes that can be predicted to enhance cellular tolerance to reactive oxygen species. (biomedcentral.com)
Bacteriophage RNA polymerases are commonly derived from T7, SP6, or T3 phages, with T7 RNA polymerase being the more prevalent choice. (techadda2.com)
In the last few years, a large number of T7-like phages have been sequenced most of which have been gathered in a new viral subfamily within the family Podoviridae . (biomedcentral.com)
All these phages, most of which have members of the Enterobacteriaceae as their host, present a highly conserved genome organization and mainly differ at sequence level through the presence or absence of nonessential genes [ 8 ]. (biomedcentral.com)
On the basis of TEM micrographs this virus resembled T7-like phages with a head diameter of about 63 nm and a noncontractile tail size approximately 13 × 8 nm. (biomedcentral.com)
Bacteriophages (phages), natural viruses known for infecting and killing bacteria, are the most diverse and abundant organisms on Earth. (cip.com.cn)

The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. (wikipedia.org)
Pimavanserin Although a international gene could be positioned between any two genes of NDV the insertion site between your P and M genes continues to be found ideal for efficient manifestation from the international proteins and replication of NDV [13 14 15 The insertion of the international gene into NDV genome raises its genome size and gene quantity. (ecologicalsgardens.com)
DSBs usually act as transcriptional factors to regulate the genes' expressions, while SSBs usually play roles in DNA replication, recombination, and repair, etc. (biomedcentral.com)
These stimulated genes inhibit viral replication in infected cells, cell proliferation, and immunomodulation. (medscape.com)

87 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" 5'UTR 119. (novoprolabs.com)
475) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(493. (novoprolabs.com)

The gradual conversion from endosymbiont to organelle during the course of evolution has clearly been accompanied by a dramatic reduction in genome size as the chloroplasts lost most of their genes to the nucleus. (frontiersin.org)
Being involved in almost all DNA and RNA metabolism processes, helicases and related proteins constitute greater than 2% of the genome. (rutgers.edu)
The genome of NDV can be a non-segmented negative-sense single-stranded RNA of 15 186 to 15 198 nucleotides including six transcriptional devices (3′-N-P-M-F-HN-L-5′) (Shape 1). (ecologicalsgardens.com)
The genome encodes a nucleocapsid proteins (N) a phosphoprotein (P) a matrix proteins (M) a fusion proteins (F) a hemagglutinin-neuraminidase proteins (HN) and a big polymerase proteins (L). Yet another proteins known as the V proteins can be made by RNA editing and enhancing from the P gene. (ecologicalsgardens.com)
Three transketolase genes have been identified in the human genome to date: transketolase (TKT), transketolase-like 1 (TKTL1) and transketolase-like 2 (TKTL2). (biomedcentral.com)
Compared to the genome of gh-1, the sequence of ϕIBB-PF7A is longer and contains more genes with unassigned function and lacks a few potentially essential and non-essential T7 genes, such as gene1.1, 3.8, and 7. (biomedcentral.com)
The genome of coliphage T7 has 56 genes encoding potential proteins [ 8 ], of which 35 have known function or functions. (biomedcentral.com)
This virus is the first T7-like bacteriophage for P. fluorescens for which the genome has been determined. (biomedcentral.com)
Together gene products 16 (gp16) and 17 (gp17) actively translocate approximately the first kbp of the T7 genome across the periplasm and into the cytoplasm. (asmblog.org)
The rest of the genome is then pulled in by transcribing RNA polymerase. (asmblog.org)
Its streamlined genome of 5386 nts encodes only 11 proteins. (asmblog.org)
In fact, sequencing data actually suggests that coronaviruses change more slowly than most other RNA viruses, because they have "proofreading" mechanisms that correct potentially fatal copying mistakes, accumulating only two single-letter mutations per month in its genome - a rate of change about half that of Influenza and one-quarter that of HIV 2 . (countlesssheep.com)
The MS2 bacteriophage consists of 180 U of the bacteriophage coat protein that encapsulates the bacteriophage genome ( 25 ). (politicalespionage.com)
Packaging of the RNA genome by coat protein is initiated by high-specificity binding to a unique site on the RNA, a single stem-loop structure, containing the initiation codon of the gene for the viral replicase. (politicalespionage.com)
The wild-type MS2 bacteriophage contains an RNA genome of approximately 3.6 kb. (politicalespionage.com)

T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. (justia.com)
Animal peptidoglycan recognition proteins homologous to Bacteriophage T3 lysozyme. (embl-heidelberg.de)
This domain is found in a family of animal peptidoglycan recognition proteins homologous to Bacteriophage T3 lysozyme [ ( PUBMED:9707603 ) ], and some bacterial homologues. (embl-heidelberg.de)

However, the current chemical synthesis technology for circular RNA can only generate circular RNAs with lengths of fewer than 70-80 nucleotides (nt). (techadda2.com)
Thus, while inculcating the UBPs in a DNA structure, their aromatic scaffolds must exhibit hydrophobic packing and must have hydrogen bond donor groups for the artificial nucleotides to interact with polymerases for their efficient incorporation in the non-template strand to bind with their UB partner in template strand [1-3]. (fortuneonline.org)

This gene encodes a T7 major capsid protein whose function is not clear. (abcam.cn)
One bacteria-induced gene encodes a proteinthat, after expression in the baculovirus system, was shown to be apeptidoglycan recognition protein (PGRP). (embl-heidelberg.de)
To permit assays of bacteriophage 80 level of sensitivity, a wild-type gene was restored to KP1345 by P1vir cotransduction (31) with Tnfrom "type":"entrez-protein","attrs":"text":"CAG12025″,"term_id":"47225542″,"term_text":"CAG12025″CAG12025, chosen by tetracycline level of MYO10 resistance, and screened for level of sensitivity to 80, creating KP1269. (informationalwebs.com)
In the present context of successful mRNA drugs, the further synthesis of linear mRNA into circular RNA (circRNA) through end-to-end connections to enhance mRNA stability and prolong protein translation time has emerged as a new hot topic in the field of mRNA therapy. (techadda2.com)
Essential amino acid residues in the single-stranded DNA-binding protein of bacteriophage T7. (wakehealth.edu)
For the first time was shown the difference of GFP reporter protein accumulation in N. rustica tissues by terminator regulation of transient gfp gene expression. (org.ua)
Typically, bacteriophages consist of an outer protein hull enclosing genetic material . (wikidoc.org)
The producing 15.4-kDa protein shares 85 N-terminal amino acids (aa) with gpE and contains a C-terminal extension encoded by the overlapping reading frame. (tam-receptor.com)
This extended protein has been designated gpE+E. METHODS and MATERIALS Bacterial and bacteriophage strains and development circumstances. (tam-receptor.com)
The vector is used to introduce a specific gene into a target cell, using the cell's mechanism for protein synthesis to produce the protein encoded by the gene. (pharmaceutical-networking.com)
During its biogenesis KdpD binds to the signal recognition particle (SRP) of Escherichia coli that consists of a 48-kDa protein Ffh and a 4.5S RNA. (nature.com)
SRP is universally conserved in its core region that consists of a ribonucleoprotein particle, the SRP RNA (4.5S RNA in E. coli ) and the protein component SRP54 (Ffh in E. coli for "fifty-four-homolog") that binds to the conserved RNA domain IV 4 . (nature.com)
The present invention also relates to a gene which hybridizes with the neurogenesis inducing gene encoding for a protein comprising at least a portion of the amino acid sequence set forth in SEQ ID NO:2. (justia.com)
In another embodiment, the present invention relates to an isolated nucleic acid encoding a protein having at least a portion of the amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 42 and in SEQ ID NO: 44. (justia.com)
The only bacteriophages known to have a lipid envelope around their protein capsids are the members of the Cystoviridae family [ 6 ]. (biomedcentral.com)
Armored L-RNA is a complex of MS2 bacteriophage coat protein and RNA produced in Escherichia coli by the induction of a two-plasmid coexpression system in which the coat protein and maturase are expressed from one plasmid and the target RNA sequence with modified MS2 stem-loop (pac site) is transcribed from another plasmid. (politicalespionage.com)
Armored RNA is a complex of MS2 bacteriophage coat protein and RNA produced in Escherichia coli by the induction of an expression plasmid that encodes the bacteriophage sequence consisting of the maturase, the coat protein, the pac site, and an exogenous RNA sequence. (politicalespionage.com)
The genes are organized from the 5′ end as follows: the maturase or A protein, the bacteriophage coat protein, a 75-amino-acid lysis protein, and a replicase subunit. (politicalespionage.com)
The armored RNA contains approximately 1.7 kb of bacteriophage RNA sequence encoding the maturase, the coat protein, and the pac site. (politicalespionage.com)

In simple terms, circular RNA is a closed single-stranded RNA molecule formed through covalent linkage at its ends. (techadda2.com)
The genetic material can be ssRNA (single stranded RNA), dsRNA , ssDNA , or dsDNA between 5 and 500 kilo base pairs long with either circular or linear arrangement. (wikidoc.org)

All transcriptional units additionally contained a 5'-untranslated region out of the 2B gene from the family of genes encoding the small subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (5'UTR RbcS2B), the coding sequence of the gfp gene and double 35S Cauliflower Mosaic Virus promoter (D35S CaMV). (org.ua)
We identified a candidate mutation presumably associated with resistance to the virus, namely the T321I mutation in the UL15 gene encoding the large terminase subunit. (bvsalud.org)

Desk 1 plasmids and strains found in this?study Plasmids pKP339 (T7-ExbB) and pK323 (T7-ExbD) were constructed the following: or PCR items (foundation pairs 582 to 1316 or 1326 to 1748 from the published series, respectively) were amplified from plasmid pKP298 containing MLR 1023 IC50 the operon. (informationalwebs.com)
Purified amplimers had been cloned into pCRII plasmids, changed into INV F operon), excluding either or gene through the operon. (informationalwebs.com)
B) … Complementation of P2 amber mutants by plasmids produced from pT7-5 and pT7-7 and expressing P2 genes beneath the control of the T7 10 promoter was assayed in stress C-2420 having the suitable plasmid pGP1-2 (43). (tam-receptor.com)
Mobile genetic elements that are common in S. aureus include bacteriophages, pathogenicity islands , plasmids , transposons , and staphylococcal cassette chromosomes. (mdwiki.org)

CONCLUSIONS: A novel family B DNA polymerase was successfully overproduced in Escherichia coli BL21(DE3). (bvsalud.org)
enGenes is an expert in the use of the T7 expression system to clone recombinant DNA using strains of Escherichia coli ( E. coli ) as an optimal system for expressing recombinant proteins. (pharmaceutical-networking.com)

The amino-terminal NG domain of SRP is bound to the ribosomal proteins uL23 and uL29, next to the tunnel exit and the carboxy-terminal M domain to the ribosomal 23S RNA 6 . (nature.com)

and c) viral replicases: HCV RNA helicase and RNA polymerase. (rutgers.edu)
Marine viruses impact global biogeochemical cycles via their influence on host community structure and function, yet our understanding of viral ecology is constrained by limitations in host culturing and a lack of reference genomes and 'universal' gene markers to facilitate community surveys. (peerj.com)
Short-read viral metagenomic studies have provided clues to viral function and first estimates of global viral gene abundance and distribution, but their assemblies are confounded by populations with high levels of strain evenness and nucleotide diversity (microdiversity), limiting assembly of some of the most abundant viruses on Earth. (peerj.com)
First, PCR-amplified marker genes are limited to a narrow subset of the viral community, and require degeneracies and amplification conditions that undermine the quantitative nature of the data ( Sullivan, 2015 ). (peerj.com)

T7 gene is transported by This plasmid in order of the temperature-sensitive repressor. (tam-receptor.com)
To stimulate production and fermentation processes, the inducer reagent IPTG can be added to prevent the lac repressor from inhibiting gene expression. (pharmaceutical-networking.com)

Radioisotopes and the Renaissance chemiluminescence immunoblot kit were purchased from NEN Life Sciences (Boston, Mass.). Immobilon-P polyvinylidene difluoride (PVDF) membrane was purchased from Millipore Corp. (Bedford, Mass.). Anti-T7 monoclonal antibody, pET expression system, and DE3 lysogenization kit were purchased from Novagen, Inc. (Madison, Wis. (informationalwebs.com)

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. (abcam.cn)
We are investigating how these steps are controlled by the sequence of the promoter and by accessory proteins to obtain a quantitative understanding of gene expression. (rutgers.edu)
6. The autogene of claim 5, wherein the autogene promoter is the T7.phi.10 promoter whose DNA sequence differs from natural T7 class III promoters by containing AG instead of GA at positions +4 and +5, where +1 is the transcriptional start site. (justia.com)
It showed that the terminator sequence has a high influence on the gene expression. (org.ua)
In another embodiment, the present invention relates to a gene comprising a DNA sequence having at least a portion of the nucleotide sequence set forth in SEQ ID NO: 1. (justia.com)

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. (pharmaceutical-networking.com)
A 3V armored L-RNA of 2,248 bases containing six gene fragments-hepatitis C virus, severe acute respiratory syndrome coronavirus (SARS-CoV1, SARS-CoV2, and SARS-CoV3), avian influenza virus matrix gene (M300), and H5N1 avian influenza virus (HA300)-was successfully expressed by the two-plasmid coexpression system and was demonstrated to have all of the characteristics of armored RNA. (politicalespionage.com)

The RNA polymerases encoded by certain bacteriophages have a simple and economical organization, Interestingly, these polymerases show homology to mitochondrial and chloroplast RNA polymerases. (rutgers.edu)

Many viruses encode their own helicases that are potential antiviral targets. (rutgers.edu)
In addition, there are 25 nonessential proteins, of which only 12 are conserved across the genus "T7-like viruses" [ 8 ]. (biomedcentral.com)
A bacteriophage (from ' bacteria ' and Greek phagein , 'to eat') is any one of a number of viruses that infect bacteria. (wikidoc.org)
Bacteriophages may have a lytic cycle or a lysogenic cycle , but a few viruses are capable of carrying out both. (wikidoc.org)
RNA Viruses, such as the SARS-CoV-2 , HIV and Influenza , tend to pick up mutations rather quickly, once they are copied inside the cells of our bodies. (countlesssheep.com)
Such a feature is unique among bacterial viruses (i.e., bacteriophages) and the mechanisms of virion envelopment within a bacterial host are largely unknown. (biomedcentral.com)

We are currently investigating a) replicative enzymes: helicases and DNA polymerase b) transcriptional enzymes: DNA-dependent RNA polymerase. (rutgers.edu)
9. The autogene of claim 8, wherein the autogene promoter is recognized by T7 RNA polymerase and the lac operator is centered at a position from +12 to +21 relative to the transcriptional start site. (justia.com)
AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes. (wakehealth.edu)

Expression of recombinant proteins under the control of the T7 promoter is up to eight times faster than basic E. coli expression under the control of RNA polymerase. (pharmaceutical-networking.com)
They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter. (biolinkk.com)

Understanding gene expression at the level of mRNA synthesis is the focus of our second research project. (rutgers.edu)
Currently, the chemical synthesis of circular RNA primarily relies on phosphoramidite chemistry and solid-phase synthesis, using naturally occurring nucleoside triphosphate derivatives as building blocks. (techadda2.com)
IVT reactions enable the cost-effective production of longer circular RNA molecules, making enzymatic synthesis the current mainstream method for circular RNA synthesis. (techadda2.com)

Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding. (jhmi.edu)

The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)
Bacterial and bacteriophage strains found in this research are shown in Table ?Table1.1. (tam-receptor.com)
Bacterial and bacteriophage strains used in this study DNA manipulations. (tam-receptor.com)
Determinants of target prioritization and regulatory hierarchy for the bacterial small RNA SgrS. (jhmi.edu)

This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. (justia.com)
Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. (justia.com)
Molecular cloning and characterization of the mouse tag7 gene encoding anovel cytokine. (embl-heidelberg.de)
Cloning of the mouse tag7 gene encoding a novel cytokine is described. (embl-heidelberg.de)

Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of bacteriophage (including T7 RNA polymerase), mitochondrial polymerases of lower eukaryotes as well as chloroplastic RpoT polymerases. (wikipedia.org)

T7 RNA polymerase binds to the T7 promoter on the expression vector to transcribe to its downstream gene of interest. (pharmaceutical-networking.com)

Such features also challenge assembly across genomic islands containing niche-defining genes that drive ecological speciation. (peerj.com)

Appearance of T7 RNA polymerase was sufficiently leaky at 33C to permit complementation of P2. (tam-receptor.com)

This happens because the little cell workers that copy the RNA (called enzymes) are sloppy workers and prone to make errors. (countlesssheep.com)

First-line and second-line antiviral drugs used to treat HSV infections, acyclovir and related compounds, as well as foscarnet and cidofovir, selectively inhibit herpesvirus DNA polymerase (DNA-pol). (bvsalud.org)
This agent competes with the natural substrate deoxyguanosine triphosphate (dGTP) to inhibit HBV polymerase activity (ie, reverse transcriptase). (medscape.com)

The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. (abcam.cn)
Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. (abcam.cn)
Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. (justia.com)
Here, we report the construction and the phenomenological characterization of synthetic gene circuits engineered with a cell-free expression toolbox that works with the seven E. coli sigma factors. (arborbiosci.com)
One of the key parameters for the development of a high level of heterologous genes expression is the efficiency of terminators used in genetic engineering, since the level of gene expression depends on its choice. (org.ua)
Study of the gfp gene expression regulation in Nicotiana rustica L. tissues by different terminators. (org.ua)
The tissues of N. rustica plants were infiltrated by the created genetic vectors for transient gene expression. (org.ua)
We detected the highest expression of the gfp gene when the Atug7 terminator was used and the lowest level with the histone H4 terminator. (org.ua)
It choice is an important step in genetic constructs creation, since terminator can be used for regulating the level of gene expression depending on the goals. (org.ua)
Nagaya S, Kawamura K, Shinmyo A, Kato K. The HSP terminator of Arabidopsis thaliana increases gene expression in plant cells. (org.ua)
Finally, we analysed the expression profiles of 15 potential immune-related genes, and the majority of them were induced more prominently with E. coli compared to Micrococcus luteus . (biomedcentral.com)
Gene expression analysis was carried out on a subset of 66 clones with real time quantitative PCR and 40 clones were positive. (silverchair.com)
In the T7 expression system, an expression vector such as pET is adapted to bring together a T7 promoter with a gene of interest that the promoter will control during downstream processing (DSP). (pharmaceutical-networking.com)

The three PACE experiments executed 45-200 rounds of evolution in 1.5-8 days and yielded T7 RNAP variants with activities on their target promoters or templates that exceeded or matched the activity of the wild-type enzyme transcribing the wild-type T7 promoter both in cells and in vitro," the team states. (genengnews.com)

Merging the frontiers: synthetic biology for advanced bacteriophage design[J]. Synthetic Biology Journal, 2023, 4(2): 283-300. (cip.com.cn)

The cytoplasmic membrane proteins ExbB and ExbD support TonB-dependent active transport of iron siderophores and vitamin B12 across the essentially unenergized outer membrane of operon, where mutations in either gene produce the same phenotype: loss of approximately 90% of TonB-dependent activity (2, 5, 10, 40). (informationalwebs.com)
Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 are associated with systemic lupus erythematosus. (wakehealth.edu)

Schematic of a T7 virion before and after ejection of the internal core proteins to extend its tail. (asmblog.org)
In brief, each T7 virion carries tail extension proteins as an internal core that is precisely positioned adjacent to the tail portal. (asmblog.org)

T4 Dnl and T4 Rnl 1 are suitable for circularizing RNA without complex secondary structures, while T4 Rnl 2 is more appropriate for linear RNA precursors with double-stranded adapter regions. (techadda2.com)

BACKGROUND: Identification and characterization of novel, faithful and processive DNA polymerases is a driving force in the development of DNA amplification methods. (bvsalud.org)

The bacteriophage molecule, but not its moth homologue, has been shown to have N-acetylmuramoyl-L-alanine amidase activity. (embl-heidelberg.de)
Light-controlled twister ribozyme with single-molecule detection resolves RNA function in time and space. (jhmi.edu)

international sequences with an excellent degree of balance so that as a RNA Pimavanserin disease there is bound probability for recombination with sponsor cell DNA. (ecologicalsgardens.com)
The start and end of every gene contain Pimavanserin control sequences referred to as gene-start (GS) and gene-end (GE) respectively. (ecologicalsgardens.com)
Although the majority of natural circular RNAs are non-coding RNAs, meaning they cannot be translated into proteins, research has shown that artificially synthesized circular RNAs with internal ribosome entry site (IRES) sequences can be translated both in vivo and in vitro. (techadda2.com)
RNase-resistant, noninfectious virus-like particles containing exogenous RNA sequences (armored RNA) are good candidates as RNA controls and standards in RNA virus detection. (politicalespionage.com)

The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction. (wakehealth.edu)

The E. coli cell's chromosomal gene can be expressed to produce T7 RNA polymerase. (pharmaceutical-networking.com)

Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes. (yeastgenome.org)

Biochemical studies reveal that helicases are nucleic acid motor proteins that use the chemical energy from NTP hydrolysis to move along DNA and RNA. (rutgers.edu)
In recent years, nucleic acid therapy involving various types of ribonucleic acids (RNAs), including messenger RNA (mRNA), small interfering RNA (siRNA), and microRNA (miRNA), has rapidly emerged as a new cornerstone of modern medicine. (techadda2.com)
In preferred embodiments, the present invention provides a nucleic acid encoding a neurogenesis inducing gene (e.g., early neurogenesis inducing gene). (justia.com)

The ability to express foreign genes in plant cells provides a powerful tool for studying the function of specific genes. (org.ua)
New secondary mesenchyme specific genes, expressed exclusively in pigment cells, were isolated from sea urchin embryos using a differential screening of a macroarray cDNA library. (silverchair.com)

In addition, the extensively folded nature of MS2 RNA ( 22 ) may make it particularly suitable for uptake into the confines of a small capsid. (politicalespionage.com)

We evaluated the 3V armored L-RNA as a calibrator for multiple virus assays. (politicalespionage.com)
In conclusion, the approach we used for armored L-RNA preparation is practical and could reduce the labor and cost of quality control in multiplex RNA virus assays. (politicalespionage.com)

Various T4 ligases derived from bacteriophages can catalyze RNA ligation reactions, including T4 DNA ligase (T4 Dnl), T4 RNA ligase 1 (T4 Rnl 1), and T4 RNA ligase 2 (T4 Rnl 2). (techadda2.com)
PCR was performed using Vent DNA polymerase in the current presence of DNA ligase (both from New Britain Biolabs). (tam-receptor.com)

More than a billion infectious bacteriophages T7 per milliliter of reaction are produced after a few hours of incubation. (arborbiosci.com)

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)

The major structural components of the P2 contractile tail are encoded in the tail gene operon. (tam-receptor.com)

In particular, the present invention provides neurogenesis inducing genes coding for Zic proteins, vectors containing such genes, host cells containing such vectors, proteins produced by such host cells, antibodies raised to such proteins, and therapeutic agents or agents for gene therapy for nervous diseases. (justia.com)

A third of the transcriptome showed similarity with previously characterized immune-related genes that included both the known and putative immune genes. (biomedcentral.com)
The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)

The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
A detailed understanding of the molecular and cellular mechanisms underlying pigment cell specification in sea urchin embryos will first require isolation of the genes responsible for this developmental process. (silverchair.com)

alternative free Sichtlochkarten zur Ordnung, as censored in attP systems, or to the calibration of available regulation systems critically Shared in cell The matrix manipulating gene are of maximization, where P-trait makes used by a biglycan of Maximum decision-making plates following to the integration of form methods where the % months come selectable or off abdominal in parameter. (mid-southrealty.com)

We also validated the upregulation of a selected subset of genes from the immune transcriptome by semi-quantitative RT-PCR. (biomedcentral.com)

It should be noted that all of these ligation methods cannot achieve circularization of large RNA fragments and cannot completely avoid side reactions associated with intermolecular end ligation. (techadda2.com)
Amplified fragments were gel purified and eluted as explained previously (28) or by chromatography on a QIAquick Spin column (Qiagen, Inc.). All cloned fragments generated by PCR amplification were sequenced to verify that no errors were launched by polymerase. (tam-receptor.com)

These circular RNAs are distributed in the cytoplasm and exhibit half-lives ranging from 18.8-23.7 hours, significantly longer than their linear homologs which have half-lives of only 4.0-7.4 hours. (techadda2.com)

In addition, IME199 DNAP has dNTP-dependent 5'-3' polymerase activity and can amplify DNA at 15-35 °C and a pH range of 5.5-9.5. (bvsalud.org)
Entecavir is a guanosine nucleoside analogue with activity against hepatitis B virus (HBV) polymerase. (medscape.com)
RNA folding and the origins of catalytic activity in the hairpin ribozyme. (jhmi.edu)

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)

Naturally occurring circular RNAs in organisms mostly arise from precursor mRNAs (pre-mRNA) through a process known as back-splicing. (techadda2.com)
The investigation of the translation mechanisms of circular RNAs in both cellular and extracellular contexts, along with the advancement of techniques for synthesizing circular RNAs in these environments, has greatly facilitated the progress of the circular RNA therapy field. (techadda2.com)

With reference to insect immunity, the ESTs have been obtained from baculovirus-infected B. mori cultured cells and pupae, but no large scale information on bacteria-induced immune genes is as yet available. (biomedcentral.com)
Bacteriophages are much smaller than the bacteria they destroy - usually between 20 and 200 nm in size. (wikidoc.org)
D'Hérelle called the virus a bacteriophage or bacteria-eater (from the Greek phagein meaning to eat). (wikidoc.org)

The Harvard team demonstrated the utility of PACE in three separate experiments to evolve T7 RNA polymerase (RNAP) variants that either recognized the T3 promoter instead of T7 or initiated transcripts with ATP or CTP instead of GTP. (genengnews.com)

Helicases are ubiquitous proteins that catalyze the separation of duplex DNA strands, the removal of secondary structures in RNA, and the reorganization of proteins bound to DNA. (rutgers.edu)

Mitochondrial DNA may also lose genes whose functions are substituted for by unrelated genes encoded in the nucleus. (biomedcentral.com)

Anatomy7

Organisms33

Chemicals and Drugs118

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes75

Disciplines and Occupations1

Information Science4

Health Care4

Phage18

Transcription16

Phages9

Replication4

Promoter for bacteriophage2

Genome15

Lysozyme3

Nucleotides2

Protein19

Single-stranded2

Subunit2

Plasmids4

Escherichia2

Ribosomal1

Viral4

Repressor2

Anti-T71

Sequence5

Plasmid2

Chloroplast1

Viruses6

Transcriptional3

Recombinant proteins2

Synthesis3

Specificity1

Bacterial4

Cloning4

Eukaryotes1

Binds1

Genomic1

Sufficiently1

Enzymes1

Inhibit2

Expression13

Promoters1

Frontiers1

Mutations2

Virion2

Double-stranded1

Characterization1

Molecule2

Sequences4

Mammalian1

Coli1

Processes1

Nucleic3

Specific genes2

Suitable1

Assays2

Ligase2

Infectious1

Phenotype1

Operon1

Vectors1

Putative2

Defects1

Molecular2

Critically1

Subset1

Fragments2

Cytoplasm1

Activity3

Genetic1

Circular2

Bacteria3

Variants1

Secondary structures1

Nucleus1