• It is ACMG's position that "Genes and their mutations are naturally occurring substances that should not be patented. (justia.com)
  • The gene (lom = lambda outer membrane) encoding this polypeptide has been mapped in the non-essential region of the lambda genome between coordinates 39.4% and 40.7% of lambda. (nih.gov)
  • As part of a huge collaborative effort called ENCODE (Encyclopedia of DNA Elements), a research team led by Cold Spring Harbor Laboratory (CSHL) Professor Thomas Gingeras, Ph.D., today publishes a genome-wide analysis of RNA messages, called transcripts, produced within human cells. (prweb.com)
  • Their analysis - one component of a massive release of research results by ENCODE teams from 32 institutes in 5 countries, with 30 papers appearing today in 3 different high-level scientific journals-- shows that three-quarters of the genome is capable of being transcribed. (prweb.com)
  • It stands in marked contrast to consensus views prior to ENCODE's comprehensive research efforts, which suggested that only the small protein-encoding fraction of the genome was transcribed, and therefore important. (prweb.com)
  • In 2003 the ENCODE project consortium was set up by the U.S. government's National Human Genome Research Institute (NHGRI) to examine the newly minted sequence of the human genome in greater depth. (prweb.com)
  • At the time, the genome was thought of as a linear molecule of DNA with "genes" being contained within isolated sections that make up just 1%-2% of its total length. (prweb.com)
  • In preliminary ENCODE results published in 2007, the researchers closely examined about 1% of the human genome. (prweb.com)
  • The additional knowledge that parts of one gene or functional RNA can reside within another were surprising, and suggested a picture of the architecture of our genome that was much more complex than previously thought. (prweb.com)
  • Most cancer-related mutations occur in regions of the genome outside of genes, but there are so incredibly many of them that it's hard to know which are actually relevant and which are merely noise," said senior author Trey Ideker, PhD, professor at UC San Diego School of Medicine and Moores Cancer Center. (ucsd.edu)
  • In fact, and fifth , Velasco argued that while new orphans are discovered with each new genome that is decoded, the trend is slowing and is suggestive that in the long run relatives for these orphans will be found: "In fact if you trend the absolute number going up, as opposed to the percentage of orphan genes in organisms, that number is going down. (evolutionnews.org)
  • Comparative genome analyses indicate that every taxonomic group so far studied contains 10-20% of genes that lack recognizable homologs in other species . (evolutionnews.org)
  • The Arabidopsis thaliana genome has three SGR genes, SGR1 , SGR2 , and STAY-GREEN LIKE ( SGRL ). (plantcell.org)
  • Both killers belong to the previously uncharacterized wtf gene family with 25 members in the reference genome. (elifesciences.org)
  • Seven genes from the SSSGP-1 family are clustered as one inverted and six tandem repeats within a 15 kb region of the genome. (pubmedcentralcanada.ca)
  • Taking into account this similarity, we searched the C. albicans genome for sequences that would encode the α pheromone gene. (asm.org)
  • Human AK2 genome location and AK2 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • It should be noted that plasmids with the NAP gene homolog also carried the relaxase gene involved in the conjugative transfer of plasmids more frequently than did those without the NAP gene homolog, implying that plasmid-encoded NAP homologs positively contribute to transmissible plasmids. (hindawi.com)
  • In this investigation we tried to transfer plasmid encoded silver and antibiotic resistance genes from Acinetobacter baumannii BL54 to E. coli k12 J53.2 and Pseudomonas (a soil microflora) by conjugation and transformation in different soil microcosms and study the survival of the isolated bacterium in each soil. (academicjournals.org)
  • Here we describe the positional cloning and molecular characterization of the LF1 gene using a bacterial artificial chromosome (BAC) library. (genetics.org)
  • Remacle, J.E., Breyer, D. & Loppes, R. Molecular cloning of the ARG7 gene of Schizosaccharomyces pombe encoding argininosuccinate lyase. (springer.com)
  • The studies show that molecular cloning technology can be used to identify potential genes, each of which encode distinct antigens that may be used for the specific immunodiagnosis of fascioliasis. (ajtmh.org)
  • We report here the molecular cloning of the gene that encodes MKK7 and demonstrate that six isoforms are created by alternative splicing to generate a group of protein kinases with three different NH 2 termini (α, β, and γ isoforms) and two different COOH termini (1 and 2 isoforms). (asm.org)
  • The coding region was 1, 758 bp and encoded a polypeptide of 586 amino acids with a deduced molecular mass of 66, 888. (go.jp)
  • A recombinant bacteriophage that formed plaques which bound MAb 17C7 was shown to contain a M. catarrhalis gene, designated uspA1, that encoded a protein with a calculated molecular weight of 88,271. (asm.org)
  • On the basis of similarity to the nitrite-responsive repressor NsrR from Nitrosomonas europaea , we propose that the yjeB gene of E. coli be renamed nsrR . (asm.org)
  • One module, encompassing all coding information except that derived from exon 1, displays striking similarity among all genes. (plantphysiol.org)
  • Because the shibire gene product and dynamin have extensive similarity, we propose that they are cognate homologues. (caltech.edu)
  • The similarity of the genes involved in the mating pathway in Saccharomyces cerevisiae and C. albicans includes at least one gene ( KEX2 ) that is involved in the processing of the α mating pheromone in the two yeasts. (asm.org)
  • SDS-PAGE analysis of other 0:54 isolates indicated that the O:54 O-polysaccharide can be co-expressed with an additional O-polysaccharide, likely encoded by chromosomal genes. (wiley.com)
  • From these data, we conclude that pWQB00 carries the rfb O:54 gene cluster and synthesis of the O:54 poiysaccharides does not require host chromosomal rfb functions. (wiley.com)
  • Rescue of the lf1 phenotype upon transformation was obtained with clones containing the complete LF1 gene as well as clones that lack the last two exons of the gene, indicating that only the amino-terminal portion of the LF1 gene product (LF1p) is required for function. (genetics.org)
  • By using bioinformatics methods, we identified the 84-amino acid ORF encoded by one of two CRNDE transcripts, previously described by our research team. (sigmaaldrich.com)
  • Myc regulates several major cellular functions, such as cell proliferation, cell adhesion, and cell size, which explains the diversity of Myc target genes found in a variety of metabolic pathways, including amino acid, nucleotide, lipid, and glucose metabolism ( www.myccancergene.org ) ( 48 ). (asm.org)
  • Because the structure of the protein encoded by the XB gene closely resembles tenascin, we term this protein tenascin-X (TN-X), and propose a simplified nomenclature system for the family of tenascins. (rupress.org)
  • The mRNA expression of the investigated genes in the human small intestine (duodenum) varies between individuals giving each one of us personal profile. (diva-portal.org)
  • Activation of the aptazyme, inserted in the 3′ UTR of the target gene, resulted in rapid self-cleavage and subsequent decay of the mRNA. (plantphysiol.org)
  • Primer extension and nuclease protection experiments demonstrated that the primary transcript is differentially spliced primarily into gamma- and beta-PPT mRNA in all tissues examined in the adult rat where the gene is expressed. (jneurosci.org)
  • beta-PPT mRNA contains all of the exons, whereas gamma-PPT mRNA lacks exon 4, which encodes part of the N-terminus of NPK. (jneurosci.org)
  • Sequencing of phage and cosmid clones and of cDNA fragments shows that the XB gene spans 65 kb of DNA, consisting of 39 exons that encode a 12-kb mRNA. (rupress.org)
  • Motifs in two of the isolated genes suggest new links between cellular regulatory mechanisms (ubiquitination and phosphorylation) and mRNA splicing and chromosome structure/function. (biologists.org)
  • Detailed antibiotic resistance gene (ARG) surveillance at regional, national, and global scale is required for the development of strategies aiming to mitigate this health threat. (nature.com)
  • The vast amount of data generated with advanced technologies by Gingeras' group and others in the ENCODE project is likely to radically change the prevailing understanding of what defines a gene, the unit we routinely use, for instance, to speak of inheritable traits like eye color or to explain the causes of and susceptibility to most diseases, running the gamut from cancer to schizophrenia to heart disease. (prweb.com)
  • Among these genes, qacA encodes the QacA Multidrug Efflux Pump that imparts decreased susceptibility to chlorhexidine, a biocide used ubiquitously in healthcare facilities. (frontiersin.org)
  • Background Genetic variants within genes involved in fibrillogenesis have previously been implicated in anterior cruciate ligament (ACL) injury susceptibility. (bmj.com)
  • In this study, a 6.7 kb plasmid from a field isolate of S. enterica serovar Borreze was shown to encode enzymes responsible for the synthesis of the O:54 polysaccharide. (wiley.com)
  • Umate P (2015) Comparison of Genes Encoding Enzymes of Sterol Biosynthesis From Plants to Orthologs in Yeast. (omicsonline.org)
  • Spinal muscular atrophy (SMA) is caused by deletion or mutation of both copies of the SMN1 gene, which produces an essential protein known as SMN. (rcsb.org)
  • For some of these cancer-related gene mutations, there are therapies that specifically target the mutation to inhibit tumor growth, an effort known as personalized or precision medicine. (ucsd.edu)
  • They replicated the non-coding mutation in cells and observed the resulting changes in gene expression. (ucsd.edu)
  • One example that stood out was a non-coding mutation affecting a gene called DAAM1," said first author Wei Zhang, PhD, a postdoctoral researcher in Ideker's lab. (ucsd.edu)
  • Strains that contain an spt7 null mutation are viable but grow very slowly and have transcriptional defects at many loci including insertion mutations, Ty elements, the INO1 gene and the MFA1 gene. (genetics.org)
  • The CYP inhibitor, cyclosporin A, partially mimics the effects of the dgt mutation in inhibiting auxin-induced adventitious root initiation in tomato hypocotyl sections and reducing the auxin-induced expression of the early auxin response genes, LeIAA10 and 11. (rti.org)
  • Anti-streptococcal mAb 10.2.3 is an autoantibody encoded by VH and VL genes, with little or no somatic mutation. (jimmunol.org)
  • Schizophrenia is a multi-factorial disease that is caused not by a single gene mutation, but reciprocation of combination of genetic and environmental elements. (pr.com)
  • The mutant ida4 has recently been shown to have a mutation in the structural gene for a 28-kD component of the subspecies a , c , and d ( LeDizet and Piperno, 1995 ). (rupress.org)
  • Here, we report an unexpected finding that ida5 has a mutation in the actin-encoding gene that results in conventional actin not being expressed at all. (rupress.org)
  • Two adrenal-specific transcripts, P450c21B (steroid 21-hydroxylase) and Y (an untranslated transcript) overlap the XB gene on the complementary strand of DNA, yielding a unique array of overlapping transcripts: a "polygene. (rupress.org)
  • In situ hybridization histochemistry experiments show that the TN-X transcript and the P450c21 and Y transcripts encoded on the complementary DNA strand are all expressed in the same cells of the human adrenal cortex. (rupress.org)
  • Some auxin responses are unaltered in dgt plants, however, and the levels, metabolism, and transport of auxin appear normal, indicating that the Dgt gene encodes a component of a specific auxin signaling pathway. (rti.org)
  • This system is highly robust and versatile, governing tightly controlled expression of transgenes and endogenous cellular genes (through shRNAs) in various primary and established cell lines in vitro, as well as in vivo in the central nervous system or in human cancer cells xenotransplanted into nude mice. (epfl.ch)
  • The goal of this article is to provide a concise methodology for construction and manipulation of this conditional lentiviral-based system, and quantitative analyses of drug-inducible transgene expression and gene knockdown both in vitro and in vivo. (epfl.ch)
  • Taking this approach into consideration, Hong incorporated Gabrb2 heterozygous (HT) transgenic mice that were bred to produce HT, wild-type (WT), and knockout (KO) mice that were weaned, genotyped using particular primers for the Gabrb2 and Neo genes (Supplementary Techniques) with water and food ad lib. (pr.com)
  • The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc), enterotoxin A (entA), and the gene segments encoding the immunoglobulin G binding region and the X region of protein A gene spa. (ebscohost.com)
  • Some S. aureus strains harbor plasmids that carry genes that affect resistance to biocides. (frontiersin.org)
  • In this study, we report that Mendel's green cotyledon gene, STAY-GREEN ( SGR ), encodes Mg-dechelatase. (plantcell.org)
  • Important goals in understanding leaf development are to identify genes involved in pattern specification, and also genes that translate this information into cell types and tissue structure. (biologists.org)
  • By fusing the IRT2 promoter to the uidA reporter gene, we show that the IRT2 promoter is mainly active in the external cell layers of the root subapical zone, and therefore provide the first tissue localization of a plant metal transporter. (nih.gov)
  • The Slc30a8 gene encodes the islet-specific zinc transporter ZnT-8, which provides zinc for insulin-hexamer formation. (pubmedcentralcanada.ca)
  • 2 ] had suggested that expression of the Slc30a8 gene, which encodes the zinc transporter ZnT-8, is restricted to pancreatic islets, specifically insulin secreting beta cells, but recent data suggest that it is also expressed in glucagon secreting alpha cells [ 3 ]. (pubmedcentralcanada.ca)
  • Evolutionary conservation of the gene family and its widespread tissue distribution suggest a broad role for proton channels in physiology and pathophysiology. (ovid.com)
  • We demonstrate here digital multiplex ligation assay (dMLA) as a low-cost targeted genomic detection workflow capable of highly-parallel screening of bacterial isolates for multiple target gene regions simultaneously. (nature.com)
  • The rat preprotachykinin (PPT) gene encoding the neuropeptides substance P (SP), neurokinin A (NKA), neuropeptide K (NPK), and neuropeptide gamma was isolated from a lambda Charon 4A genomic library. (jneurosci.org)