Gene dna catalytic. Medical search. Frequent questions

Here, we present a crystal structure of a precatalytic complex of a DNA polymerase with bound substrates that include the primer 3'-OH and catalytic Mg2+. (rcsb.org)
Comparison with two new structures of DNA polymerase beta lacking the 3'-OH or catalytic Mg2+ is described. (rcsb.org)
The DNA polymerase catalytic subunit (open reading frame [ORF] 30) gene was amplified by using herpesvirus universal primers ( 6 ). (cdc.gov)
During evolution, this protein naturally lost its catalytic activity in Drosophila where Pcif1 is, like its human counterpart, expressed in the nucleus and associated with the C-terminal domain of RNA polymerase (RNA Pol II). (cea.fr)
DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. (nih.gov)
Mutations/polymorphisms in the 55 kDa subunit of DNA polymerase epsilon in human colorectal cancer. (nih.gov)
The solution structure of the amino-terminal domain of human DNA polymerase epsilon subunit B is homologous to C-domains of AAA+ proteins. (nih.gov)
Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. (nih.gov)
My research was focused on DNA Polymerase Zeta. (creighton.edu)
This is an error-prone DNA polymerase. (creighton.edu)
A review article about iron-sulfur clusters in DNA polymerase and primase enzymes. (creighton.edu)
All organisms-bacteria, archaea, and eukaryotes-have a transcription initiation factor that contains a structural module that binds within the RNA polymerase (RNAP) active-center cleft and interacts with template-strand single-stranded DNA (ssDNA) in the immediate vicinity of the RNAP active center. (osti.gov)
Simple and cost-effective, the selection process can sample a very large pool of DNA (up to 1,000 trillion molecules), amplify the desired sequence by the polymerase chain reaction, and introduce mutations to improve performance. (nanowerk.com)
Genes corresponding to a B-family type polymerase ('polB') and an HNH nuclease ('HNH') are marked. (elifesciences.org)
The replisome contains activities that separate the strands and hold them apart for synthesis by the replisome version of DNA polymerase, called DNA polymerase III in bacteria. (blogspot.com)
Deoxyribonucleic Acid (DNA) Recall that the replisome contains a DNA polymerase III holoenzyme dimer with two core complexes that can catalyze polymerization. (blogspot.com)
Each primer is extended from its 3′ end by DNA polymerase I to form an Okazaki fragment, as shown in the Figure. (blogspot.com)
The use of short RNA primers gets around the limitation imposed by the mechanism of DNA polymerase, namely, that it cannot initiate DNA synthesis de novo . (blogspot.com)
The primers are synthesized by a DNA dependent RNA polymerase enzyme called primase-the product of the dna G gene in E. coli . (blogspot.com)
The primosome, along with DNA polymerase III, is part of the replisome. (blogspot.com)
DNA polymerase III catalyzes synthesis of DNA in the 5′ → 3′ direction by extending each short RNA primer. (blogspot.com)
The steps are carried out by the combined action of DNA polymerase I and DNA ligase. (blogspot.com)
The syndrome is caused by an intronic mutation in POLA1, the gene encoding the catalytic subunit of DNA polymerase-α (Pol-α), which is responsible for Okazaki fragment synthesis during DNA replication. (jci.org)

For practical purposes, depending on the enzyme activity and the presence of mutations in the G6Pase and T genes, respectively, GSD type I may be subdivided into 2 major forms. (medscape.com)
It is an autosomal recessive disorder in which there is an AGL gene mutations which causes deficiency in glycogen debranchinging enzyme and limited storage of dextrin. (medscape.com)
Mutations in genes for telomerase cause a wide spectrum of disorders including, premature aging syndromes. (uab.edu)
I would like to get involved studing DNA mutations in cancer in human and mouse lines, as well as possibly study the effects of chemotherapy drugs on stems cells and cancer cells. (creighton.edu)
Prof. Lukas Dow, biochemistry, and his team published a study in Nature on Aug. 16, detailing a new, more specific gene editing tool that they created to study cancer mutations through preclinical mice models. (cornellsun.com)
The team combined Cas9, an enzyme that cuts DNA, and guide RNA, a type of RNA that determines which DNA region Cas9 cuts, with apolipoprotein B mRNA editing enzyme, catalytic polypeptide - an enzyme commonly known as APOBEC that creates single base mutations in DNA. (cornellsun.com)
Frequent inactivating mutations of the ATM gene have been reported in patients with rare sporadic T cell prolymphocytic leukaemia (T-PLL), B cell chronic lymphocytic leukaemia (B-CLL), and most recently, mantle cell lymphoma (MCL). (bmj.com)
The presence of inactivating mutations, together with the deletion of the normal copy of the ATM gene in some patients with T-PLL, B-CLL, and MCL, establishes somatic inactivation of the ATM gene in the pathogenesis of lymphoid malignancies, and strongly suggests that ATM functions as a tumour suppressor. (bmj.com)
Resistance of NNRTIs occurs through mutations of the reverse transcriptase gene in the viral genome. (antimicrobe.org)
In addition, mutations in mitochondrial DNA have been associated with an increased risk of the disease. (bmglabtech.com)
Mutations in the PINK1 gene (also known as PARK6) are associated with early onset Parkinson's disease. (bmglabtech.com)
This phenotype is reminiscent of hypomorphic mutations in MCM4, which encodes a component of the minichromosome maintenance (MCM) helicase complex that is functionally linked to Pol-α during the DNA replication process. (jci.org)
Tobacco smoking is linked to "stop-gain mutations" (SGMs) that interfere with the formation of tumor suppressor genes, which help keep abnormal cells in check, new research suggests . (medscape.com)
Our study shows that tobacco smoking signatures in DNA generate these harmful protein-truncating mutations that contribute to the development of cancer and its increasing complexity over time," senior author Jüri Reimand, PhD, Ontario Institute for Cancer Research, Toronto, Canada, told Medscape Medical News . (medscape.com)
These SGM mutations frequently affect cancer tumor suppressor genes such as TP53 , FAT1 , and APC , keeping them from fully forming to produce proteins that prevent abnormal cells from growing and causing cancer. (medscape.com)
We have known for decades that smoking causes DNA mutations that in turn cause cancer. (medscape.com)

Gene expression in benzene-exposed workers by microarray analysis of peripheral mononuclear blood cells: induction and silencing of CYP4F3A and regulation of DNA-dependent protein kinase catalytic subunit in DNA double strand break repair. (cdc.gov)
We found that DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and mammalian target of rapamycin (mTOR) complex 2 (mTORC2) were both required for UVB-induced Akt Ser-473 phosphorylation in keratinocytes. (biomedcentral.com)

The TERT gene encodes the catalytic sub-unit of telomerase, which polymerizes telomeric DNA. (uab.edu)
Arden and associates have described the cloning of a novel cDNA that encodes an islet-specific G-6-Pase catalytic subunit-related protein (IGRP) (Arden SD, Zahn T, Steegers S, Webb S, Bergman B, O'Brien RM, Hutton JC: Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic subunit related protein (IGRP). (diabetesjournals.org)
TOP2A encodes an enzyme which is implicated in almost any process of DNA metabolism including transcription, replication, movement and untangling [ 3 , 8 , 9 ], which catalyze the passage of two DNA duplexes across each other to resolve the entanglements and coiling of cellular DNA [ 10 ]. (jcancer.org)
The ATM gene encodes a large protein that belongs to a family of kinases possessing a highly conserved C-terminal kinase domain related to the phosphatidylinositol 3-kinase domain. (bmj.com)
4 - 7 The ATM gene encodes a nuclear phosphoprotein of approximately 350 kDa (3056 amino acids) and is ubiquitously expressed. (bmj.com)
The BRAF (v-raf murine sarcoma viral oncogene homolog B) gene encodes a protein that belongs to the Raf family of serine/threonine protein kinases. (hindawi.com)
The class Ib RNR operon in B. anthracis encodes genes for the catalytic NrdE protein, the tyrosyl radical metalloprotein NrdF, and the flavodoxin protein NrdI. (ibecbarcelona.eu)

In living organisms, gene expression is a complex process that results in the production of proteins from the genome in a time- and space-regulated manner. (cea.fr)
This expression is strictly dependent on the synthesis of messenger RNAs transcripts from the DNA molecule and their subsequent translation into proteins by the ribosomes. (cea.fr)
This study demonstrates the importance to not restrict the function of enzymes to their sole catalytic role in the living world and the interest of thoroughly examining the evolution of proteins in eukaryotes in order to discover unsuspected or hidden mechanisms of action. (cea.fr)
8 , 9 The protein is a member of a novel family of large proteins, which show sequence homology to the catalytic domain of phosphatidylinositol 3 kinase, 3 and are implicated in cell cycle regulation, signal transduction, and the response to DNA damage. (bmj.com)
4 , 10 There is evidence to suggest that these proteins respond to DNA damage by phosphorylating one or more substrates, including p53, c-Abl, and replication protein A (RPA), to recruit proteins to regions of DNA repair and/or to activate radiation signal transduction pathways. (bmj.com)
Increased levels of reactive oxygen species can damage the molecular fabric of the cell including lipids, proteins, and DNA. (bmglabtech.com)

The genes that group I introns interrupt differ significantly: They interrupt rRNA, mRNA and tRNA genes in bacterial genomes, as well as in mitochondrial and chloroplast genomes of lower eukaryotes, but only invade rRNA genes in the nuclear genome of lower eukaryotes. (wikipedia.org)
In higher plants, these introns seem to be restricted to a few tRNA and mRNA genes of the chloroplasts and mitochondria. (wikipedia.org)
Sequence-specific cleavage of Huntington mRNA by catalytic DNA. (bcm.edu)
Furthermore, Reimand said, the team identified APOBEC (apolipoprotein B mRNA editing catalytic polypeptide-like) enzyme-driven SGMs, "which may help decipher the role of APOBECs in tumorigenesis and progression. (medscape.com)

In agreement with these observations, the Pcif1 protein is distributed all along the polytene chromosomes at the level of active transcription sites and interferes with chromatin-dependent gene expression regulation. (cea.fr)
The lack of catalytic activity of Pcif1 in Drosophila reveals a new mechanism of gene expression regulation. (cea.fr)
Reversible Regulation of Promoter and Enhancer Histone Landscape by DNA Methylation in Mouse Embryonic Stem Cells. (ca.gov)
DNA methylation is one of a number of modes of epigenetic gene regulation. (ca.gov)
DNA topoisomerases, ubiquitously present in eukaryotes, archaebacteria and Eubacteria, are necessary for the regulation of DNA topology in various cellular procedures [ 3 , 4 ]. (jcancer.org)

In mice, mutation of PCIF1 encoding gene causes deregulation of the expression of a set of genes and a reduced body weight. (cea.fr)
The work of the scientists shows that mutation of Pcif1 gene in Drosophila results in a deregulation of the expression of a set of genes, reduced body weight and a significant drop in fertility which indicates an important role for this protein in the physiology of the organism. (cea.fr)
A novel mutation in the POLE2 gene causing combined immunodeficiency. (nih.gov)
The association between mutation of the ATM gene and a high incidence of lymphoid malignancy in patients with AT, together with the development of lymphoma in Atm deficient mice, supports the proposal that inactivation of the ATM gene may be of importance in the pathogenesis of sporadic lymphoid malignancy. (bmj.com)
Inhibition of DNA-PKcs activity via its inhibitor NU7026, a dominant-negative kinase-dead mutation, RNA interference (RNAi) or gene depletion led to the attenuation of UVB-induced Akt Ser-473 phosphorylation. (biomedcentral.com)
UVB-induced complexation between DNA-PKcs and mTORC2 components was also abolished by NU7026 and DNA-PKcs mutation. (biomedcentral.com)

During DNA replication, a molecular machine called a replisome forms at the replication fork where the two strands of DNA are separating. (blogspot.com)
The complex has two sliding clamps that bind the complex to the strands of DNA so that DNA replication is highly processive. (blogspot.com)
The overall mechanism of DNA replication is called semidiscontinuous to emphasize the different mechanisms for replicating each strand. (blogspot.com)
As the replication fork progresses, the parental DNA is unwound, and more and more single-stranded DNA becomes exposed. (blogspot.com)
The replication process requires the DNA duplex to be first melted and then separated into two single-stranded templates for DNA polymerases to synthesize as complement strands. (sflorg.com)
Unlocking the secret of DNA replication is key to understanding the mystery of life. (sflorg.com)
In eukaryotes, the enzyme responsible for unzipping DNA duplex during replication was originally identified as the minichromosome maintenance protein complex (MCM) genes from wine brewing yeast by our collaborator Professor TYE Bik-Kwoon at Cornell University in 1983. (sflorg.com)
In cells, to initiate DNA replication, MCM2-7 complex must be first assembled into a head-to-head double hexamer (DH) encircling duplex DNA at thousands of places along each chromosome. (sflorg.com)
Furthermore, when this initial open structure is disturbed, MCM2-7 DHs can no longer assemble onto DNA, leading to a complete suppression of DNA replication initiation. (sflorg.com)
The atomic-resolution cryo-EM structures enabled direct visualization of the initial DNA melting, which is crucial for us to understand the molecular mechanism of DNA replication,' said Dr DANG Shangyu, Assistant Professor of Division of Life Science, HKUST, 'This study also demonstrates the importance of collaboration. (sflorg.com)
Activation of DNA-PKcs by UV is dependent on ATR (Ataxia telangiectasia mutated and Rad3 related) kinase and is important for replication stress [ 12 ]. (biomedcentral.com)
RNR is a universal enzyme that provides the four essential deoxyribonucleotides needed for DNA replication and repair. (ibecbarcelona.eu)

The RAG endonuclease consists of RAG1, which contains the active site for DNA cleavage, and RAG2, an accessory factor whose interaction with RAG1 is critical for catalytic function. (nih.gov)
It modulates the topological states of DNA by transient cleavage, strand passing and religation of double-stranded DNA resulting in decatenation of intertwined DNA molecules and relaxation of supercoiled DNA [ 8 , 9 ]. (jcancer.org)
The presence of uranyl causes catalytic cleavage of the DNA and release of the fluorophore, resulting in a dramatic increase of fluorescence intensity. (nanowerk.com)
The CRISPR/Cas system uses guide RNAs (gRNAs) to direct sequence-specific DNA cleavage. (harvard.edu)
Exogenous control of mammalian gene expression via modulation of RNA self-cleavage. (bcm.edu)

these genes code for the enzyme telomerase. (uab.edu)
The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. (nih.gov)
DNA methyltransferases (DNMTs) constitute the enzyme family responsible for the establishment and maintenance of DNA methylation patterns. (nature.com)
This enzyme is actually a multicomponent system, the catalytic subunit of which was recently cloned. (diabetesjournals.org)
The catalytic activities of these two COMT variants, expressed in E. coli, were similar, but the Met-108 enzyme was more thermolabile already at physiological temperature (37 °C). The reported existence of a common polymorphism of the human COMT gene coding for a thermolabile low activity, COMT(L), and a thermostable high activity, COMT(H), is discussed in light of the different thermostability of the two enzyme forms. (lu.se)
The three-dimensional crystal structure of the DnaG catalytic domain revealed its folding and active site are distinct from the well studied polymerases, suggesting that it may employ a novel enzyme mechanism. (blogspot.com)

Taken together, these results strongly suggest that DNA-PKcs-mTORC2 association is required for UVB-induced Akt Ser-473 phosphorylation and cell survival, and might be important for tumor cell transformation. (biomedcentral.com)
These studies provide new insights into the regulatory effect exerted by JMJD2B on tumor growth through the modulation of p53 target genes. (escholarship.org)
An analysis of DNA from more than 12,000 tumor samples across 18 different cancer types showed how tobacco smoking truncates tumor suppressors, effectively deactivating them. (medscape.com)
The authors used artificial neural croarrays is to study changes in gene ex- .nature organ the tumor arose from, how big it networks (ANNs) to identify gene-ex- pression that accompany changes in is, and what it looks like after it has pression signatures associated with spe- cell physiology, such as during develop- been imbedded in wax and colored cific subtypes of tumors. (lu.se)

Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
Evidence also points to multiple genes being linked to mitochondrial dysfunction in Alzheimer's disease. (bmglabtech.com)
In Parkinson's disease, a strong link between mitochondrial dysfunction and disease-associated genes has been documented. (bmglabtech.com)
[ 11 ] In the CALERIE phase 1 study, however, we demonstrated that 6 months of 25% CR in healthy human s increased the expression of genes involved in mitochondrial biogenesis and mitochondrial DNA (mtDNA) copy number. (medscape.com)

According to their different acting mechanisms, DNA topoisomerases can be classified as type I and type II enzymes [ 3 , 6 ]. (jcancer.org)
To accomplish his goal, Dow moved to optimizing base editing enzymes and developing a system that allows him to turn the expression of these enzymes on and off with doxycycline, an antibiotic also typically used to regulate gene expression. (cornellsun.com)
Cancer Research UK DNA Repair Enzymes Group, Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer BN1 9RQ, U.K. [email protected] [email protected] [email protected]. (rcsb.org)
The signal of aptamer binding to target or the catalytic activity of DNA enzymes can be transduced using fluorescent dyes. (genelink.com)
They found that SGMs were strongly enriched in DNA signatures of tobacco smoking, APOBEC enzymes, and reactive oxygen species. (medscape.com)
The CAZy database describes the families of structurally-related catalytic and carbohydrate-binding modules (or functional domains) of enzymes that degrade, modify, or create glycosidic bonds. (lu.se)

We screened a mouse BAC library with this cDNA to isolate the IGRP gene, which spans approximately 8 kbp of genomic DNA. (diabetesjournals.org)
3 , 4 The ATM gene is located at 11q22-23, spans 184 kb of genomic DNA, and has 66 exons. (bmj.com)

Baranovskiy AG, Tahirov TH, 'Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes. (creighton.edu)
DNA polymerases catalyze chain elongation exclusively in the 5′ → 3′ direction. (blogspot.com)

The sizes of these exons are 254 (I), 110 (II), 112 (III), 116 (IV), and 1284 (V) bp, similar to those of the G-6-Pase catalytic subunit gene. (diabetesjournals.org)
The IGRP and G-6-Pase catalytic subunit gene promoters show a reciprocal pattern of activity, with the IGRP promoter being approximately 150-fold more active than the G-6-Pase promoter in HIT cells. (diabetesjournals.org)
In situ hybridization analysis of AMPA receptor subunit gene expression in the developing rat spinal cord. (bcm.edu)

Thus, epigenetic mechanisms as long-term determinants of gene expression are a plausible link between genetics and environment, which may instigate the manifestation and/or progression of IBD. (nature.com)

Studies carried out by scientists at IRIG, in collaboration with the University of Geneva, on the fruit fly model Drosophila melanogaster have revealed the role of Pcif1 in the control of gene expression, despite the fact that this protein has completely lost its RNA methyltransferase activity compared to its mammalian counterpart PCIF1. (cea.fr)
Most mammalian genes are equally expressed from the two inherited parental alleles. (harvard.edu)
Recent advances in DNA microar- bones, studying mammalian entrails or ray technologies have made it possible searching with divining rods. (lu.se)

The expression of photosynthesis and chlorophyll synthesis related genes were significantly reduced under both types of stresses, which limit the metabolic processes and thus help prolong survival under extreme conditions. (frontiersin.org)
However, cell wall synthesis related genes were up-regulated under drought stress and down-regulated under flooding stress. (frontiersin.org)
Since the two strands of DNA are antiparallel, synthesis using one template strand occurs in the same direction as fork movement, but synthesis using the other template strand occurs in the direction opposite fork movement. (blogspot.com)
This limitation presents no difficulty for leading-strand synthesis since once DNA synthesis is under way nucleotides are continuously added to a growing chain. (blogspot.com)
About once every second, primase catalyzes the synthesis of a short RNA primer using this single-stranded DNA as a template. (blogspot.com)
The reaction proceeds in three steps: removal of the RNA primer, synthesis of replacement DNA, and sealing of the adjacent DNA fragments. (blogspot.com)

In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)
We find that genome-wide DNA demethylation alters occupancy of histone modifications at both promoters and enhancers. (ca.gov)
Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)

A recurrent BCAM-AKT2 fusion gene leads to a constitutively activated AKT2 fusion kinase in high-grade serous ovarian carcinoma. (bcm.edu)
Members of this kinase family have been shown to function in DNA repair and cell cycle checkpoint control following DNA damage. (bmj.com)

The most frequent site of alkylation in DNA is the O 6 position of guanine, which forms cross-links between adjacent strands of DNA, leading to cell death. (aacrjournals.org)
A number of studies have indicated that DNA topoisomerases play an essential role in the DNA world through allowing DNA double helices or strands to cut across each other [ 4 , 5 ]. (jcancer.org)
Dow explained that the guide RNA takes Cas9 to a specific region in the genome, allowing Cas9 to then cut one of the DNA strands at the targeted site. (cornellsun.com)

Explore the normal functions of human genes and the health implications of genetic changes. (medlineplus.gov)
Genetic variants in the DNA methyltransferase 3 A (DNMT3A) locus have been associated with inflammatory bowel disease (IBD). (nature.com)
As the genetic material inside every cell nucleus, DNA is indispensable for the maintenance of genetic stability and integrity. (jcancer.org)
Despite these difficulties, the gene editing tool presents favorable opportunities to understand the effects of single-base genetic changes on tumors through models and determine which therapy is effective against cancer. (cornellsun.com)
The AT locus was mapped to the chromosomal region 11q22-23 using genetic linkage analysis in the late 1980s and the causative gene was identified by positional cloning several years later. (bmj.com)
During each cell division, our genome DNA, the blueprint of genetic information, is accurately replicated. (sflorg.com)
Finally, we found that both DNA-PKcs and SIN1 were associated with apoptosis resistance of UVB radiation, and inhibition of them by NU7026 or genetic depletion significantly enhanced UVB-induced cell death and apoptosis. (biomedcentral.com)

The DNA repair protein O 6 -methylguanine-DNA methyltransferase (MGMT) plays an important role in cellular resistance to alkylating agents. (aacrjournals.org)
A cellular DNA-repair protein, namely O 6 -methylguanine-DNA methyltransferase (MGMT) protein, reverses alkylation at the O 6 position of guanine, thereby inhibiting the lethal cross-linking and bringing about resistance to alkylating agents ( 2, 3 ). (aacrjournals.org)
Whereas DNMT1 is the key maintenance methyltransferase, DNMT3A and DNMT3B represent the main de novo methyltransferases, as they can bind unmethylated DNA and establish novel DNA methylation marks not only during early development but also in differentiated cells in a signal-dependent manner 9 . (nature.com)

Then the terminal G (omega G) of the intron swaps the exoG and occupies the G-binding site to organize the second ester-transfer reaction: the 3'-OH group of the upstream exon in P1 is aligned to attack the 3' splice site in P10, leading to the ligation of the adjacent upstream and downstream exons and release of the catalytic intron. (wikipedia.org)
The exon/intron structure of the IGRP gene has been mapped and, as with the gene encoding the liver/kidney G-6-Pase catalytic subunit, it is composed of five exons. (diabetesjournals.org)
TOP2A (DNA topoisomerase II alpha) gene, mapped to chromosome 17q12-q21, covers approximately 27.5 kb and includes 35 exons, encoding a 170 kDa protein [ 7 ]. (jcancer.org)

Ren L, Liu J, Gou K, Xing C. Copy number variation and high expression of DNA topoisomerase II alpha predict worse prognosis of cancer: a meta-analysis. (jcancer.org)
Tyrosyl-DNA phosphodiesterase 2 (TDP2) is a 5'-tyrosyl DNA phosphodiesterase important for the repair of DNA adducts generated by non-productive (abortive) activity of topoisomerase II (TOP2). (rcsb.org)

After screening different histone lysine methyltransferases and demethylases, we identified JMJD2B/KDM4B as a p53-inducible gene in response to DNA damage. (escholarship.org)

Intron Group I Intron Sequence and Structure Database Splice site Nuclear introns Group II intron Group III intron Twintron LtrA Cyclic di-GMP-II riboswitch, where there is an example of a riboswitch acting together with a group I intron to regulate the expression of a gene Nielsen H, Johansen SD (2009). (wikipedia.org)

Reverse transcriptase directs the polymerization of DNA from viral RNA. (antimicrobe.org)

This study highlights an important contribution for Pcif1 in gene expression and physiology of the organism independently of any catalytic activity. (cea.fr)
These effects require DNMT catalytic activity. (ca.gov)
Structure and promoter activity of an islet-specific glucose-6-phosphatase catalytic subunit-related gene. (diabetesjournals.org)
The IGRP gene transcription start site was mapped by primer extension analysis, and the activity of the IGRP gene promoter was analyzed in both the islet-derived HIT cell line and the liver-derived HepG2 cell line. (diabetesjournals.org)
TOP2A is one of the isoenzymes which can mediate the catalytic activity of type II topoisomerases [ 6 ]. (jcancer.org)
Differential gene activity is controlled by transcription factors but also dependent on the underlying chromatin structure, especially on covalent histone modifications. (escholarship.org)

Additionally, the gene editing tool has a domain called the uracil glycosylase inhibitor, which prevents the newly changed bases from being reverted back to cytosine. (cornellsun.com)
DNA glycosylase/AP lyase, H2TH DNA-binding [Interproscan]. (ntu.edu.sg)

DNMT3A is part of the epigenetic machinery physiologically involved in DNA methylation. (nature.com)
G9a-dependent H3K9 methylations (G9a) have been shown to mediate epigenetic silencing of several tumours suppressor genes including DSC3, MASPIN, and CDH1. (omicsonline.org)
This pathway describes progression of a serrated precursor lesion, often followed by the onset of epigenetic instability involving promoter methylation and silencing of key tumour suppressor genes, and accounts for 15%-20% of sporadic colorectal cancer [ 1 , 2 ]. (hindawi.com)
To elucidate whether epigenetic effects play a role for these gene expression changes, we performed whole genome bisulphite sequencing to assess DNA methylation patterns in the lungs 56 days after exposure to MWCNTs. (cdc.gov)
Taken together, the present study revealed that MWCNTs exert epigenetic effects in the lungs of exposed animals, potentially driving the subsequent gene expression changes. (cdc.gov)

Loss of function of the Parkin gene (PARK2) leads to impaired mitophagy (destruction of mitochondria by the cell's homeostatic machinery) which produces a buildup of damaged mitochondria. (bmglabtech.com)

The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. (nih.gov)
Title : An intronic variant in the CELF4 gene is associated with risk for colorectal cancer Personal Author(s) : Teerlink, Craig C.;Stevens, Jeff;Hernandez, Rolando;Facelli, Julio C.;Cannon-Albright, Lisa A. (cdc.gov)

2015). Importantly, the long isoform, GABP b 1L, regulates a restricted subset of genes, and in a mouse xenograft model of glioblastoma regulates expression from mutant TERT promoters (Mancini et al. (uab.edu)
DNA methylation promotes H3K27me3 deposition at bivalent promoters, while opposing H3K27me3 at silent promoters. (ca.gov)

TERT expression is associated with metastasis from thin primaries, exhausted CD4+ T cells in melanoma and with DNA repair across cancer entities. (nih.gov)

Meanwhile, this DNA-PKcs-SIN1 complexation by UVB was dependent on epidermal growth factor receptor (EGFR) activation, and was disrupted by an EGFR inhibitor (AG1478) or by EGFR depletion. (biomedcentral.com)
JMJD2B induction attenuates the transcription of key p53 transcriptional targets including p21, PIG3 and PUMA, and this modulation is dependent on the catalytic capacity of JMJD2B. (escholarship.org)

By analyzing the single-molecule properties of engineered motors, we demonstrate that the non-catalytic domain has a key role in the motility mechanism by acting as a 'foothold' that allows Kar3 to bias translocation towards the minus end. (elifesciences.org)

An initial open structure (IOS) is formed upon binding of human MCM double hexamer (hMCM-DH) to origin DNA. (sflorg.com)
The team also found that the MCM2-7 DHs are loaded onto DNA at tens of thousands of sites across the human genome, which are mutually exclusive with loci of active transcription. (sflorg.com)
pendent of those associated with cretory ducts, columnar epithelia the KRAS gene is more frequent- other key mechanistic characteris- lining the gastrointestinal tract and ly mutated in human cancer, which tics of IARC Group 1 carcinogens. (who.int)

We studied FVII gene poly- then activates factors IX and X leading to morphisms in healthy Tunisians with the the generation of thrombin [ 2 ]. (who.int)

10976 in exon 8 in the catalytic region of ing factors that may increase cardiovascu- the FVII gene and an insertion of a decanu- lar disease. (who.int)

Two interspecific backcross DNA mapping panels were used to unambiguously localize the IGRP gene (map symbol G6pc-rs) to the proximal portion of mouse chromosome 2. (diabetesjournals.org)

The molecular details of the nucleotidyl transferase reaction have remained speculative, as strategies to trap catalytic intermediates for structure determination utilize substrates lacking the primer terminus 3'-OH and catalytic Mg2+, resulting in an incomplete and distorted active site geometry. (rcsb.org)

A proportion of BRAF mutant lesions will methylate a DNA mismatch repair gene, MLH1 , which leads to the onset of microsatellite instability (MSI) [ 3 ]. (hindawi.com)

These genes comprise 289 Transcription Factors (TFs) representing Basic Helix-loop Helix (bHLH), Ethylene Response Factors (ERFs), myeloblastosis (MYB), No apical meristem (NAC), and WRKY amino acid motif (WRKY) type major families known to be involved in the mechanism of stress tolerance. (frontiersin.org)

To address this, a single gene copy that produces APOBEC is integrated into the cell's genome and controlled by gene expression regulator doxycycline in order to determine how much APOBEC is produced. (cornellsun.com)

While most DNA is double stranded, the catalytic DNA Lu's research group uses has a single strand region that can wrap around like a protein. (nanowerk.com)

The genes for glycoprotein B (gB) (ORF33), glycoprotein 2 (gp2) (ORF71), and glycoprotein D (gD) (ORF72) were amplified by using primers specific for EHV-9. (cdc.gov)
We used PCR to amplify a part of the gB gene of the giraffe herpesvirus, and we used EHV-1 specific primers for sequencing. (cdc.gov)

The sequence of the 1,066-bp segment of the giraffe herpesvirus ORF30 gene was 99.5% identical to EHV-9 and 94.6% identical to EHV-1, which indicates that the giraffe herpesvirus was most closely related to EHV-9. (cdc.gov)
To search for the unique sequence of DNA that could distinguish uranyl from other metal ions, the researchers used a combinatorial approach called in vitro selection. (nanowerk.com)

Alteration (amplification or deletion) of TOP2A gene was also significantly related to shorter survival of cancer patients. (jcancer.org)
Significantly, we discovered that DNA-PKcs was associated with SIN1 in cytosol upon UVB radiation, and this complexation appeared required for Akt Ser-473 phosphorylation. (biomedcentral.com)

A unique feature of our uranium sensor is that it contains a small piece of DNA, the same basic building blocks of our genes,' said Lu, who also is a researcher at the university's Beckman Institute for Advanced Science and Technology, and at the Center of Advanced Materials for the Purification of Water with Systems. (nanowerk.com)

Here, we used biolayer interferometry and pulldown assays to identify regions of RAG1 necessary for interaction with RAG2 and to measure the RAG1-RAG2 binding affinity (KD ∼0.4 μM) (where RAG1 and RAG2 are recombination activating genes 1 or 2). (nih.gov)
Mini-RAG1 consists primarily of the catalytic center and the residues N-terminal to it, but it lacks a zinc finger region in RAG1 previously implicated in binding RAG2. (nih.gov)

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes1

Information Science2

Polymerase23

Mutations16

Protein kinase catalytic subunit2

Encodes7

Proteins6

MRNA4

Regulation5

Mutation6

Replication12

Cleavage5

Enzyme6

Tumor4

Mitochondrial4

Enzymes6

Genomic2

Polymerases2

Subunit gene3

Mechanisms1

Mammalian3

Synthesis6

Genome-wide3

Kinase2

Strands3

Genetic7

Methyltransferase3

Exons3

Topoisomerase2

Response to DNA damage1

Intron1

Reverse transcriptase1

Activity6

Glycosylase2

Epigenetic5

Mitochondria1

Colorectal cancer2

Promoters2

TERT1

Dependent2

Molecule1

Human3

Activates1

Exon1

Chromosome1

Molecular1

Onset1

Transcription Factors1

Controlled by gene expression1

Single strand1

Primers2

Sequence2

Significantly2

Purification1

RAG12