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  • tRNA gene
  • KplE1, HK620 and numerous (pro)phages that integrate at the same site in enterobacteria genomes (such as the argW tRNA gene) all share a highly conserved recombination module. (mdpi.com)
  • mutations
  • NPM1 deletion, hypermethylation, or mutations were not found in any of the 23 patients with isolated 5q- while loss of one copy of NPM1 was found in 7/30 patients with 5q- associated with complex karyotype. (haematologica.org)
  • Mutations in this gene or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. (wikipedia.org)
  • In a computational analysis of genetic variation and mutations in 2,472 human orthologs of known essential genes in the mouse, Georgi et al. (wikipedia.org)
  • Of the 8,041 individuals with complete knock-outs, 6,885 were estimated to be homozygotes, 1,249 were estimated to be compound heterozygotes (i.e. they had both alleles of a gene knocked out but the two alleles had different mutations). (wikipedia.org)
  • When mutations in the FBN-1 or FBN-2 genes occur, significant deformations can result from the damage to the extracellular matrix. (wikipedia.org)
  • Mutations in the FBN1 gene are associated with Marfan syndrome and its variant Marfanoid-progeroid-lipodystrophy syndrome, autosomal dominant Weill-Marchesani syndrome, isolated ectopia lentis, MASS phenotype, and Shprintzen-Goldberg syndrome. (wikipedia.org)
  • mutants
  • I know that counterselectable markers are often instrumental for the construction of mutants when under appropriate growth conditions, a counterselectable gene promotes the death of the microorganisms harboring it. (protocol-online.org)
  • Insertion mutants that are still able to survive or grow are not in essential genes. (wikipedia.org)
  • alleles
  • Genetic analysis of healthy individuals confirmed the expected presence of two copies, or alleles, of the gene for osteoprotegerin. (innovations-report.com)
  • DQ2 There are many other gene-alleles within the haplotype, including more than 250 coding loci that produce transcripts. (wikipedia.org)
  • Multigene haplotypes following standard dynamics only exist in robust populations for a short time, the average distance between genes of about 200,000 nts, which means that over 250 generations (~5000 years) one expects 1/2 of adjacent genes to have new gene-alleles, unless the genes are small and very close together. (wikipedia.org)
  • mice
  • Scientists working with mice have found that by removing a single gene they can turn normally cautious animals into daring ones, mice that are more willing to explore unknown territory and less intimidated by sights and sounds that they have learned can be dangerous. (biopsychiatry.com)
  • Using genetic engineering, the scientists removed the gene from mice and bred a line of the animals, all missing the same gene. (biopsychiatry.com)
  • To be sure that it was the gene change and not some other quality that explained the differences, the researchers tested hearing and pain sensitivity in the altered mice. (biopsychiatry.com)
  • In the current study, the researchers deleted the leucine zipper-bearing kinase ( LZK ) gene in astrocytes of one group of injured mice, which decreased the cells' injury response and resulted in a larger wound on the spinal cord. (genengnews.com)
  • Conversely, the scientists overexpressed the gene in other injured mice, which stimulated the cells' injury response and resulted in a smaller scar. (genengnews.com)
  • Overexpressing the gene in uninjured mice also activated the astrocytes, confirming LZK as a trigger for astrogliosis. (genengnews.com)
  • Inducible LZK gene deletion in astrocytes of adult mice reduced astrogliosis and impaired glial scar formation, resulting in increased lesion size after spinal cord injury. (genengnews.com)
  • To explore the role of interleukin-2 (IL-2) in T cell proliferation, and to circumvent the IL-2 deficiency autoimmune syndrome of conventional il2 gene deletion, mice were created to allow conditional il2 gene deletion when treated with the estrogen analog, tamoxifen (TAM) as adults. (frontiersin.org)
  • encodes
  • In our preliminary studies, we have observed that approximately two-thirds of patients with WAGR syndrome have deletion of the gene which encodes brain-derived neurotrophic factor (BDNF), and that BDNF haploinsufficiency is associated with obesity and with parent reports of hyperphagia and impaired nociception, suggesting that BDNF may play an important role in human energy balance as well as pain sensation. (clinicaltrials.gov)
  • This gene encodes a preproprotein that is processed to yield both alpha and beta chains, which subsequently combines as a tetramer to produce haptoglobin. (wikipedia.org)
  • This gene encodes the basic form of complement factor 4, part of the classical activation pathway. (wikipedia.org)
  • defects
  • In familial or sporadic CPHD cases, the most common causes are PIT1 and PROP1 gene defects [ 2 ]. (hindawi.com)
  • The team now is evaluating other patients worldwide with varying forms of JPD, who so far do not appear to have any defects in the osteoprotegerin gene. (innovations-report.com)
  • h) Counting genes with essential domains and those that lead to growth-defects when disrupted as essential, and those who lead to growth-advantage when disrupted as non-essential. (wikipedia.org)
  • marker
  • The problem is i am using a suicide vector pFS100 a derivative of pGP704 carrying a 'pir' dependent origin of replication from plasmid R6K and gene encoding resistance to ampicillin and kanamycin but no sacB gene marker in it. (protocol-online.org)
  • We have now created a triple gene deleted GAP by additionally removing the SAP1 gene (Pf p52(-)/p36(-)/sap1(-) GAP) and employed flippase (FLP)/flippase recognition target (FRT) recombination for drug selectable marker cassette removal. (nih.gov)
  • a ) Schematic of strategy for deleting the SAP1 gene in a Pf p52 - /p36 - KO clone lacking the HsDHFR marker. (nih.gov)
  • We have developed an efficient gene deletion system for C. parapsilosis based on the repeated use of the dominant nourseothricin resistance marker (caSAT1) and its subsequent deletion by FLP-mediated, site-specific recombination. (jci.org)
  • Sox9 blocked osteochondroprogenitor cells were found to express osteoblast marker genes, reprogramming the cells into the osteoblastic lineage. (wikipedia.org)
  • BDNF
  • Researchers found five individuals with BDNF deletions, all of whom were obese, had a mild-moderate intellectual impairment, and had a mood disorder. (psychcentral.com)
  • The results of the new study, published in the Archives of General Psychiatry , reveal for the first time the link between BDNF deletion, cognition, and weight gain in humans. (psychcentral.com)
  • Linking a deletion in BDNF conclusively to mood and anxiety really tells us that it is possible to dissect the biological pathways involved in determining how we feel and act. (psychcentral.com)
  • All of the participants in our study had mild-moderate intellectual disability, but most people with these cognitive problems do not have psychiatric problems - so what is it about deletion of BDNF that affects mood? (psychcentral.com)
  • embryonic
  • Transcription factor genes playing a role in the development of hypothalamus and pituitary are pituitary transcription factor 1 (PIT1), Prophet of Pit-1 (PROP1), LIM Homeobox 3 (LHX3), LIM Homeobox 4 (LHX4), and HESX Homeobox 1 (HESX1) which all are known to be important for organ commitment and embryonic pituitary cell differentiation. (hindawi.com)
  • The FBN-1 gene is involved in a variety of embryonic developmental programs. (wikipedia.org)
  • Researchers
  • Gene therapy to create daredevil warriors is likely to remain the province of screenwriters, but the new findings may help researchers design novel drugs to treat a wide array of conditions, from disabling anxiety in social settings to the sudden flights of poisoned memory that can persist in the wake of a disaster, an attack or the horror of combat. (biopsychiatry.com)
  • The researchers found the fear-related gene by analyzing brain tissue, in particular the tiny prune-shaped region called the amygdala, which previous studies had shown to be especially active when animals and humans were afraid or anxious. (biopsychiatry.com)
  • The researchers next tested the gene that makes osteoprotegerin, a protein discovered only a few years ago. (innovations-report.com)
  • variation
  • found strong, purifying selection and comparatively reduced levels of sequence variation, indicating that these human genes are essential too. (wikipedia.org)
  • steroid
  • It has also been used with the steroid sulfatase gene. (wikipedia.org)
  • Modular variations of the human major histocompatibility complex class III genes for serine/threonine kinase RP, complement component C4, steroid 21-hydroxylase CYP21, and tenascin TNX (the RCCX module). (wikipedia.org)
  • deficiency
  • Interestingly, although little boy with combined pituitary hormone deficiency has adrenocorticotropic hormone (ACTH) deficiency, his elder sisters with the same gross PROP1 deletion have no ACTH deficiency. (hindawi.com)
  • enzyme
  • Probable DNA dC->dU-editing enzyme APOBEC-3B is a protein that in humans is encoded by the APOBEC3B gene. (wikipedia.org)
  • FBN1 is a 230-kb gene with 65 coding exons that encode a 2,871-amino-acid long proprotein called profibrillin which is proteolytically cleaved near its C-terminus by the enzyme furin convertase to give fibrillin-1, a member of the fibrillin family, and the 140-amino-acid long protein hormone asprosin. (wikipedia.org)
  • therapy
  • In an e-mail message, Dr. Joseph LeDoux, a neuroscientist at New York University wrote: "While we are a long ways away, it is possible in the future that we will be able to identify amygdala-specific genes that can be used to play a role in amygdala-specific drug therapy. (biopsychiatry.com)
  • These results have implications for treating several brain conditions through gene therapy targeting astrocytes. (genengnews.com)
  • absence
  • The total loss or absence of a gene. (healthboard.com)
  • In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. (wikipedia.org)
  • PAO1
  • Now both my vector (pFS100) as well as PAO1 has no sacB gene. (protocol-online.org)
  • f) Deduced by comparison of the two gene essentiality datasets obtained independently in the P. aeruginosa strains PA14 and PAO1. (wikipedia.org)
  • Genetically
  • A next-generation genetically attenuated Plasmodium falciparum parasite created by triple gene deletion. (nih.gov)
  • We previously generated a genetically attenuated parasite (GAP) by deleting the P52 and P36 genes in the NF54 wild-type (WT) strain of Plasmodium falciparum (Pf p52(-)/p36(-) GAP). (nih.gov)
  • found
  • They found that a protein called stathmin, produced by the stathmin gene, was highly concentrated in the amygdala but hard to detect elsewhere in the brain. (biopsychiatry.com)
  • In genetic analysis, we found a gross deletion involving PROP1 coding region. (hindawi.com)
  • This gene along with APOBEC3A have been in recent years found associated with mutagenesis of several cancers. (wikipedia.org)
  • human
  • The new study helps provide a better understanding of human behavior and mood by clearly identifying genes associated with mental disorders. (psychcentral.com)
  • In these individuals, a total of 1,171 of the 19,135 human (RefSeq) genes (6.1%) were completely knocked out. (wikipedia.org)
  • Alteration
  • Alteration of the gene encoding the PROP1 may affect somatotropes, thyrotropes, and lactotropes, as well as gonadotropes and corticotropes. (hindawi.com)
  • genetic
  • The genetic operation wherein a gene from the parent's DNA is removed from the offspring's DNA . (everything2.com)
  • We performed genetic analysis of PROP1 gene in a Turkish pedigree with three siblings who presented with short stature. (hindawi.com)
  • result
  • If the original gene was beneficial, the result might be lethal to the offspring. (everything2.com)
  • g) The original result of 271 essential genes has been corrected to 261, with 31 genes that were thought to be essential being in fact non-essential whereas 20 novel essential genes have been described since then. (wikipedia.org)
  • region
  • Although not contained within any common deleted region on 5q, NPM1 can be deleted in 5q- cases with large chromosomal deletions. (haematologica.org)
  • The KLRC (NKG2) gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed on NK cells. (wikipedia.org)
  • large
  • Conclusions Full exome sequencing of a large multigenerational AxSpA family revealed a 9-bp deletion in SEC16A segregating in the majority of affected family members. (bmj.com)
  • experiments
  • Such experiments have led to the conclusion that the absolutely required number of genes for bacteria is on the order of about 250-300. (wikipedia.org)
  • cell
  • Finding from the new study, published recently in Cell Reports (" Leucine Zipper-Bearing Kinase Is a Critical Regulator of Astrocyte Reactivity in the Adult Mammalian CNS "), shows that turning on a gene inside astrocytes results in a smaller scar and, potentially, more effective recovery from injury. (genengnews.com)
  • role
  • The association of this deletion within the SEC16A gene with AxSpA is particularly interesting given that the gene product plays a role in protein transport from the endoplasmic reticulum (ER) to the Golgi and mediates COPII vesicle formation at the transitional ER. (bmj.com)