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  • 1997
  • Plant virus diseases pose severe constraints to the production of a wide range of economically important crops worldwide (Agrios 1997). (apsnet.org)
  • cucumber mosai
  • Transgene translatability increases effectiveness of replicase-mediated resistance to cucumber mosaic virus. (nih.gov)
  • Cucumber mosaic virus is restricted from entering minor veins in transgenic tobacco exhibiting replicase-mediated resistance. (nih.gov)
  • Specificity of replicase-mediated resistance to cucumber mosaic virus. (nih.gov)
  • Replicase-mediated resistance to cucumber mosaic virus in transgenic plants involves suppression of both virus replication in the inoculated leaves and long-distance movement. (nih.gov)
  • amplicons
  • DNA fragments (seeds) having the characteristics of amplicons, which are useful for amplifying genes of interest, have been isolated from Marek's disease viruses of poultry. (patentgenius.com)
  • Candidate genes for use with the subject amplicons include those which encode immunogenic proteins and other beneficial economic traits desired in commercial poultry lines. (patentgenius.com)
  • Sequences
  • An amplicon plasmid ( Fig. 2A ) is a standard Escherichia coli plasmid carrying one origin of virus replication (OriS) and one packaging signal (pac) from HSV-1, in addition to the transgenic sequences of interest (Vlazny & Frenkel 1981, Spaete & Frenkel 1985, Deiss et al. (bioline.org.br)
  • Determination of the complete nucleotide sequence of T7 DNA , 39,937 base pairs, revealed coding sequences for more than fifty T7 proteins and the arrangement of signals that direct gene expression. (bnl.gov)
  • avian
  • Carter, M.J., Mahy, B. W.J.: Incomplete avian influenza vims contains a defective noninterfer- ing component. (springer.com)
  • 9. The DNA of claim 5 wherein said foreign DNA sequence is an avian gene capable of being amplified concurrent with formation of concatemers by said amplicon. (patentgenius.com)
  • vaccine
  • The improvement of methods for efficient delivery and regulated expression of genetic material in mammalian cells has been a major objective of molecular and cellular biology, gene therapy and vaccine development over the last 25 years and it is still an area of intensive research. (bioline.org.br)
  • One problem is that HIVsurface proteins such as gp120 mutate at a high rate.Scientist may be able to construct a vaccine using an HIV with a defective form of the viral gene nef. (dnatube.com)
  • mRNA
  • This plant defense system results in degradation of mRNA produced both by the transgene and the virus. (apsnet.org)
  • diseases
  • The design and construction of the JDV vector system should facilitate the study of the lentivirology and pathogenesis of the diseases associated with JDV or other bovine virus infections. (edu.au)
  • Genetic engineering brings new hope for the effective control of plant virus diseases. (apsnet.org)
  • Advances in Understanding Plant Viruses and Virus Diseases. (nih.gov)
  • tumor
  • We have developed a strategy for viral-mediated tumor therapy, where herpes simplex virus (HSV) replication and associated cytotoxicity are limited to a specific cell-type by the regulated expression of an essential immediate-early gene. (nii.ac.jp)
  • Gene Thorapy : Targeting tumor cell for destruction' Human Cell. (nii.ac.jp)
  • sequence
  • The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. (microbiologyresearch.org)
  • Any errors made by the polymerase enzyme during the first and the third steps are not subjected to proof reading, the result being pronounced sequence variability".137 Hence, as long ago as 1973, it was concluded that the above phenomena "will prove a stumbling block to any genetic analysis of RNA tumour viruses"138 (RNA tumour viruses=retrovirus). (tig.org.za)
  • expression
  • When compared to the transmission-competent SeV in vivo, no difference in gene expression was observed at the time of peak expression. (cfgenetherapy.org.uk)
  • These proteins are delivered into the cell during infection and can trigger cell signalling and cellular responses and, consequently, may have a transient impact on cell homeostasis or gene expression. (bioline.org.br)
  • Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia," PNAS U S A. Jun. (freepatentsonline.com)
  • deletion
  • G92A contains an albumin enhancer/promoter-ICP4 transgene within the thymidine kinase (tk) gene of the HSV-1 ICP4 deletion mutant d120. (nii.ac.jp)
  • vector
  • The development of an F-defective nontransmissible SeV, which can still efficiently mediate gene transfer to the airway epithelium, represents the first important step towards the use of a cytoplasmic RNA viral vector in clinical trials of gene therapy. (cfgenetherapy.org.uk)
  • We have developed a gene transfer vector system based on JDV that has three components. (edu.au)
  • To our knowledge, this is the first such vector system developed from a cattle virus. (edu.au)
  • helper virus
  • Cell lines which produced in excess of 10(5) helper virus-free transducing units/ml were generated by trans-complementation of the replication defect using a BHK-21-derived cell line expressing the Bel-1 transactivator. (uzh.ch)
  • 1. A substantially biologically pure amplicon derived from a Marek's disease virus as a DNA fragment thereof, wherein said amplicon contains all cis-acting functions required for DNAreplication, and in the presence of helper virus, said amplicon infects host cells and replicates, thereby forming high molecular weight concatemers of DNA. (patentgenius.com)
  • transgenic
  • Compared to conventional breeding for virus resistance, genetic engineering provides a quicker and more precise technology to obtain plants that are resistant to viruses, although most transgenic virus-resistant plants are still under laboratory development. (apsnet.org)
  • Figure 2.1 Transgenic papaya plants show resistance (right) while non-transgenic plants (left) are susceptible to papaya ringspot virus under field conditions. (apsnet.org)
  • Replicase-mediated resistance to potato virus Y in transgenic tobacco plants. (nih.gov)
  • tumour
  • n = 6/group) with LIVP-GFP (5 × 10 7 PFU of virus in 0.1 mL of IMDM immediately and 4 days after tumour implantation). (biomedcentral.com)
  • The anti-tumour activity of LIVP-GFP is a result of direct oncolysis of tumour cells in case of melanoma B-16 because the virus effectively replicates and destroys these cells, and virus-mediated activation of the host immune system followed by immunologically mediated destruction of of tumour cells in case of lymphosarcoma RLS-40. (biomedcentral.com)
  • Genetic
  • The concept of pathogen derived resistance (Sanford and Johnston, 1985) has stimulated research on obtaining virus resistance through genetic engineering. (apsnet.org)
  • transgene
  • In addition, it contains the E.coli LacZ gene upstream of the ICP4 transgene, under control of the tk promoter. (nii.ac.jp)
  • 1999
  • In: 15th Meeting of the International Working Group on Legume Viruses, 15 - 17 August 1999, Fremantle, Western Australia. (edu.au)
  • newly
  • Jembrana disease virus (JDV) is a newly isolated and characterised bovine lentivirus. (edu.au)
  • When technicians move the nucleus of an adult cell into an egg, the clone will survive only if the egg successfully reprograms the newly installed genes to function as they would in an embryo. (discovermagazine.com)