• Assays showed that multiple proteins can bind to this sequence and that one of these is the transcription regulator delta-EF1 (dEF1). (usda.gov)
  • Electromobility shift assays showed that the transcription regulator delta-EF1 (dEF1) is one of the proteins that recognize the E box region. (usda.gov)
  • We then screened for COUP-TFI-interacting proteins from a cortisol-producing adenoma cDNA library using a yeast two-hybrid system and identified a novel RING finger-containing protein which can function as a coregulator for COUP-TFI. (elsevierpure.com)
  • This domain occurred 46 times on human genes ( 181 proteins). (umbc.edu)
  • These proteins are intracellular transcription factors that directly regulate gene expression in response to lipophilic molecules. (indigobiosciences.com)
  • In this newly discovered pathway, high levels of fat were found to interfere with two key transcription factors - proteins that switch genes on and off. (blogspot.com)
  • These transcription factors, FOXA2 and HNF1A, are normally required for the production of an enzyme called GnT-4a glycosyltransferase that modifies proteins with a particular glycan (polysaccharide or sugar) structure. (blogspot.com)
  • Numerous cellular proteins detect DNA damage and induce senescence , a permanent change of state characterized by morphological and gene expression changes. (massgenomics.org)
  • In the CYTOPLASM, I-kappa B proteins bind to the transcription factor NF-KAPPA B. Cell stimulation causes its dissociation and translocation of active NF-kappa B to the nucleus. (bvsalud.org)
  • J Virol, 79 2005), a DNA sequence that is responsible for the regulation of estrogen responsive genes in animals. (usda.gov)
  • This tested for transcription regulation by these members of the nuclear receptor family that also recognize a consensus ERE. (usda.gov)
  • Cotransfection assays, using expression vectors for thyroid receptor (TR) or chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) and the CAV promoter driving expression of EGFP, were used to test for transcription regulation by these members of the nuclear receptor family that also recognize a consensus ERE. (usda.gov)
  • The rat HMGCS2 gene contains elements that control its multihormonal regulation and tissue specificity ( 11 - 14 ). (aacrjournals.org)
  • Chicken ovalbumin upstream promoter-transcription factors (COUP-TFs) and steroidogenic factor-1 (SF-1) play key roles in the transcriptional regulation of steroidogenic P450 genes. (elsevierpure.com)
  • This positive regulation of HNF4alpha by COUP-TFII was confirmed by adenoviral overexpression of human (h)COUP-TFII which increased HNF4alpha mRNA in 832/13 INS-1 cells and in mouse pancreatic islets. (lonza.com)
  • As is the case with many eukaryotic genes, regulation of the chick ovalbumin (Ov) gene involves both positive and negative modulation. (umn.edu)
  • Gel mobility shift analyses with the CAR element suggested that it may play a role in the developmental regulation of the Ov gene. (umn.edu)
  • In addition to these transcription factors, several peptide hormones (allatotropins, allatostatins and short neuropeptide F) and neurotransmitters (the biogenic amines octopamine, dopamine and glutamate) are also involved in the regulation of CA activity 16 . (nature.com)
  • The gene for cholesterol 7 alpha-hydroxylase (CYP7A1) contains a sequence at nt -149 to -118 that was found to play a large role in determining the overall transcriptional activity and regulation of the promoter. (omeka.net)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
  • I Introductory Chapters -- Ch. 1 General organization of nuclear receptors -- Ch. 2 DNA recognition by nuclear receptors -- Ch. 3 Ligand binding -- Ch. 4 Molecular mechanisms of transcriptional regulation -- Ch. 5 RXR subordination in heterodimers -- Ch. 6 Antagonist action -- Ch. 7 Nuclear receptors: platforms for multiple signal integration -- Ch. 8 Deregulation in disease and novel therapeutic targets -- Ch. 9 Genes and evolution -- Ch. 10 Conclusions -- Sect. (edu.au)
  • Our loss- and gain-of-function studies, as well as the observed genetic interactions among Dorsocross, tinman and pannier , suggest that co-expression of these three genes in the cardiac mesoderm, which also involves cross-regulation, plays a major role in the specification of cardiac progenitors. (biologists.com)
  • The cholesterol 7 alpha -hydroxylase gene (CYP7A1) plays an important role in regulation of bile acid biosynthesis and cholesterol homeostasis. (omeka.net)
  • The results indicate that COUP-TFs negatively regulate the transcriptional activity of SF-1, a steroidogenic cell-specific activator of various steroidogenic P450 genes. (elsevierpure.com)
  • The receptors function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner. (embl.de)
  • 7. Valcárcel J, Singh R, Zamore PD, Green MR. The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA. (prolekarniky.cz)
  • Since glucocorticoids are essential for life, other cellular signaling pathways strongly regulate GR actions in many different ways, such as physical interaction via their effector transcription factors and epigenetic modifications including phosphorylation and acetylation. (brainimmune.com)
  • Understand Nuclear Receptors through our introduction to this family of intracellular transcription factors that regulate gene expression. (indigobiosciences.com)
  • COUP-TFII (COUP transcription factor 2), also known as NR2F2 (nuclear receptor subfamily 2, group F, member 2) is a protein that in humans is encoded by the NR2F2 gene. (wikipedia.org)
  • The chicken infectious anemia virus (CAV) gene promoter fused to the gene for enhanced green fluorescent protein (EGFP) was used to measure virus activity in response to estrogen. (usda.gov)
  • The standard vascular markers used in pathologic differential diagnosis of vascular conditions include von Willebrand factor (vWF), factor VIII-associated protein, platelet endothelial cell adhesion molecule-1 (CD31), human hematopoietic progenitor cell antigen (CD34), v- ets avian erythroblastosis virus E26 oncogene homolog (ERG), human herpes virus 8 (HHV-8), latency-associated nuclear antigen (LANA1), podoplanin (D2-40), and PROX1. (basicmedicalkey.com)
  • The human GR protein is produced from 9 exons of the GR gene and is composed of 3 major subdomains, each of which has specific structural and functional properties. (brainimmune.com)
  • Analysis of the human Iguratimod (T 614) calreticulin-1 gene promoter also revealed four specificity protein 1 (SP-1) sites and a single activator protein 1 (AP-1) site. (academicediting.org)
  • Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
  • The COUP acronym stands for chicken ovalbumin upstream promoter. (wikipedia.org)
  • Furthermore, enzalutamide-induced YAP1 expression is mediated through the function of chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII) at the transcriptional and the post-transcriptional levels. (nih.gov)
  • Cotransfection assays were done, using expression vectors for thyroid receptor (TR) or chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) to increase the levels of these hormone receptors in the cells containing the CAV promoter driving expression of EGFP. (usda.gov)
  • Ear2 is an orphan nuclear hormone receptor that belongs to the chicken ovalbumin upstream promoter-transcription factors (COUP-TF) gene family [ 14 ]. (biomedcentral.com)
  • Knockout mice with heterozygous deletion of the Chicken Ovalbumin Upstream Promoter- Transcription Factor II (COUP-TFII) gene or complete disruption of the Hepatocyte Nuclear Factor 4 alpha (HNF4alpha) gene in pancreatic beta cells have similar insulin secretion defects leading us to hypothesize that there is transcriptional cross-talk between these two nuclear receptors. (lonza.com)
  • Sensenbaugh, KR & Sanders, MM 1999, ' Multiple promoter elements including a novel repressor site modulate expression of the chick ovalbumin gene ', DNA and Cell Biology , vol. 18, no. 2, pp. 147-156. (umn.edu)
  • Sanders, Michel M. / Multiple promoter elements including a novel repressor site modulate expression of the chick ovalbumin gene . (umn.edu)
  • Nkc2.5) increases calreticulin expression in the heart while chicken ovalbumin upstream promoter-transcription factor 1(COUP-TF1) binds to the Nkx2.5 binding CACNG1 site and suppresses transcription from the calreticulin promoter.43 44 In the present study calreticulin expression markedly decreases at 24 h of estradiol stimulation suggesting this decline is due to the presence of an inhibitory factor. (academicediting.org)
  • DNA-binding domain of chicken ovalbumin upstream promoter transcription factors (COUP-TFs) is composed of two C4-type zinc fingers. (umbc.edu)
  • Bioinformatic analysis and molecular biological characterization revealed that VEGF-C is negatively regulated by an orphan nuclear receptor, chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII). (endometriosistreatmentreport.com)
  • HR is usually conditioned by single dominant genes, while field resistance is generally controlled by multiple genes ( Yang, 2013 ). (frontiersin.org)
  • Functional analyses reveal that YAP1 positively regulates numerous genes related to cancer stemness and lipid metabolism and interacts with COUP-TFII to form a transcriptional complex. (nih.gov)
  • HMGCS2 , the gene that regulates ketone body production, is expressed in liver and several extrahepatic tissues, such as the colon. (aacrjournals.org)
  • 4. Graham PL, Yanowitz JL, Penn JK, Deshpande G, Schedl P. The translation initiation factor eIF4E regulates the sex-specific expression of the master switch gene Sxl in Drosophila melanogaster. (prolekarniky.cz)
  • Steroid or nuclear hormone receptors constitute an important superfamily of transcription regulators that are involved in widely diverse physiological functions, including control of embryonic development, cell differentiation and homeostasis. (embl.de)
  • These response elements position the receptors, and the complexes recruited by them, close to the genes of which transcription is affected. (embl.de)
  • Transcription factors 3: nuclear receptors. (embl.de)
  • Here, using the hemimetabolous insect Blattella germanica , we show that nuclear hormone receptors Seven-up-B (BgSvp-B) and Fushi tarazu-factor 1 (BgFTZ-F1) have essential roles in the tissue- and stage-specific activation of adult CA JH-biosynthetic activity. (nature.com)
  • COUP-TFs homodimerize or heterodimerize with retinoid X receptor (RXR) and a few other nuclear receptors and bind to a variety of response elements that are composed of imperfect AGGTCA direct or inverted repeats with various spacings. (umbc.edu)
  • COUP-TFs are generally considered to be repressors of transcription for other nuclear hormone receptors such as retinoic acid receptor (RAR), thyroid hormone receptor (TR), vitamin D receptor (VDR), peroxisome proliferator activated receptor (PPAR), and hepatocyte nuclear factor 4 (HNF4). (umbc.edu)
  • In brief, vascular endothelial growth factor-A (VEGF-A), which is a part of a large superfamily of growth factors, is required for differentiation of endothelial precursor cells. (basicmedicalkey.com)
  • These diverse actions of glucocorticoids are mediated by the glucocorticoid receptor (GR), which belongs to the nuclear receptor superfamily and functions as a hormone-dependent transcription factor. (brainimmune.com)
  • COUP-TFs are orphan members of the steroid/thyroid hormone receptor superfamily. (umbc.edu)
  • Like other members of the nuclear receptor (NR) superfamily of ligand-activated transcription factors, COUP-TFs have a central well conserved DNA binding domain (DBD), a variable N-terminal domain, a flexible hinge and a C-terminal ligand binding domain (LBD). (umbc.edu)
  • Identification and characterization of the host resistance as well as regulatory genes can provide insights into understanding the mechanisms of host resistance and the use of host resistance to develop new cultivars with durable resistance. (frontiersin.org)
  • We postulate that in activated T cells COUP-TF1 is usually either not expressed or is Iguratimod (T 614) unable to bind to regulatory regions of the calreticulin gene. (academicediting.org)
  • Therefore, this complex regulatory network requires precise control of gene expression patterns at both transcriptional and post-transcriptional levels in order to ensure proper maturation, differentiation, and formation of blood and lymphatic vessels. (nature.com)
  • Studies in several vertebrate models and Drosophila have uncovered a surprising degree of evolutionary conservation of regulatory mechanisms and genes that control early cardiogenesis. (biologists.com)
  • The glucocorticoid receptor (GR) stimulates COUP-TFII-induced transactivation while COUP-TFII represses the GR transcriptional activity. (wikipedia.org)
  • BACKGROUND: Rare genetic variants and genetic variation at loci in an enhancer in SOX17 (SRY-box transcription factor 17) are identified in patients with idiopathic pulmonary arterial hypertension (PAH) and PAH with congenital heart disease. (bvsalud.org)
  • Since no genetic mutations in steroidogenic enzyme genes have been identified as yet, the dysregulated expression at the transcription level may be crucial. (elsevierpure.com)
  • In attempts to determine whether genetic variations in the oxytocin signaling system contribute to ASD susceptibility, seven recent reports indicated association of common genetic polymorphisms in the OXTR gene with ASD. (biomedcentral.com)
  • These results suggest that genetic variations in TNF, TGFB1, PTGS1 and PTGS2 genes contribute to DA susceptibility. (cdc.gov)
  • In mammals, SOX18, together with COUP-TFII/NR2F2, activates the expression of Prox1, a master regulator in lymphatic identity and development. (bvsalud.org)
  • Knockdown studies have also suggested an involvement of Sox18, Coup-tfII/Nr2f2, and Prox1 in zebrafish lymphatic development. (bvsalud.org)
  • Nuclear receptor subfamily 2 group F member 2 (NR2F2 or COUP-TF2) encodes a transcription factor which is expressed at high levels during mammalian development. (childrensmercy.org)
  • The variants seen were predicted loss of function, including a nonsense variant inherited from a mildly affected mosaic mother, missense and a large deletion including the NR2F2 gene. (childrensmercy.org)
  • Surprisingly, two positive sites were found, one of which is the binding site for the estrogen-responsive transcription factor δ-EF1. (umn.edu)
  • Finally, YAP1, COUP-TFII, and miR-21 are detected in the extracellular vesicles (EVs) isolated from EnzaR cells and sera of patients. (nih.gov)
  • Coexpression with COUP-TF1 but not TR or a control RSV promoter vector was found to decrease expression of EGFP by 50-60% in DF-1 and LMH cells. (usda.gov)
  • In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
  • Moreover, real-time RT-PCR showed upregulation of endogenous renin transcription in As4.1 cells over-expressing Rasd1. (biomedcentral.com)
  • The prospero related homeobox-1 (PROX1) transcriptional factor and vascular endothelial growth factor receptor-3 (VEGFR3) are responsible for their differentiation into lymphatic endothelial cells. (basicmedicalkey.com)
  • Transfection studies showed that SF-1 activated and COUP-TFs repressed the transcription of bovine CYP17 gene promoter from the CRS2 element in a mutually exclusive manner in Y-1 cells. (elsevierpure.com)
  • Here we show specific HNF4alpha activation of a reporter plasmid containing the COUP-TFII promoter region in transfected pancreatic beta cells. (lonza.com)
  • Dominant negative suppression of HNF4alpha function decreased endogenous COUP-TFII expression and specific inactivation of COUP-TFII by short interfering (si)RNA caused HNF4alpha mRNA levels to decrease in 832/13 INS-1 cells. (lonza.com)
  • Therefore COUP-TFII could contribute to the control of insulin secretion through the complex HNF4alpha/maturity-onset diabetes of the young 1 (MODY1) transcription factor network operating in beta cells. (lonza.com)
  • Both factors are highly expressed in adult CA cells. (nature.com)
  • The genes encoding the enzymes involved in both modules are highly enriched, or even exclusively expressed, in the CA cells 9 . (nature.com)
  • It is tempting to speculate that COUP-TF1 an established suppressor of steroid receptor binding21 45 inhibits estrogen-dependent activation of calreticulin in resting T cells. (academicediting.org)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Herein, we identified that vascular endothelial growth factor (VEGF)-C, a potent lymphangiogenic factor, is up-regulated in endometriotic cells and contributes to increased lymphangiogenesis. (endometriosistreatmentreport.com)
  • A stable association of the endogenous HNF4alpha with a region of the COUP-TFII gene promoter that contains a direct repeat 1 (DR-1) binding site was revealed by chromatin immunoprecipitation. (lonza.com)
  • Many of the NRs act as ligand-inducible transcription factors, responding to endogenous and exogenous chemicals. (indigobiosciences.com)
  • In an early step, the Dorsocross genes act in parallel with tinman to activate the expression of pannier , a cardiogenic gene encoding a Gata factor. (biologists.com)
  • In all these organisms, cardiac induction in the lateral mesoderm involves the concerted action of signals mediated by Bmp, Wnt and Fgf family members, which act upon cardiogenic genes encoding NK homeodomain, Gata and T-box transcription factors (reviewed by Zaffran and Frasch, 2002 ). (biologists.com)
  • Overall, the numbers of differentially expressed genes (DEGs) were higher in the resistant tomato line PI 114490 than in the susceptible line OH 88119, and the numbers of DEGs were higher at 6 DPI than at 6 HPI. (frontiersin.org)
  • Taken together, these results suggest that YAP1 might be a crucial factor involved in the development of enzalutamide resistance and can be an alternative therapeutic target in prostate cancer. (nih.gov)
  • Additionally, dihydroflavonol 4-reductase ( DFR ) (LOC101293459 and LOC101293749) and anthocyanidin 3-O-glucosyltransferase ( BZ1 ) (LOC101300000), which are crucial structural genes in the anthocyanidin biosynthetic pathway, had significantly up-regulated expression levels in the two FN interspecific hybrids. (biomedcentral.com)
  • Thus, organisms cannot survive in the absence of glucocorticoid action, as observed in the mouse model in which the glucocorticoid receptor (GR) gene is genetically deleted [3]. (brainimmune.com)
  • 3. Gempe T, Beye M. Function and evolution of sex determination mechanisms, genes and pathways in insects. (prolekarniky.cz)
  • Mutations in the transcription factor-coding gene SOX18, the growth factor-coding gene VEGFC and its receptor-coding gene VEGFR3/FLT4 cause primary lymphedema in humans. (bvsalud.org)
  • The gene encoding the oxytocin receptor, OXTR , is a strong functional ASD candidate gene based on its known role in modulating social behavior (Ebstein et al. (biomedcentral.com)
  • COUP-TFII interacts with GATA2 to inhibit adipocyte differentiation. (wikipedia.org)
  • Pancreatic islet beta cell differentiation and function are dependent upon a group of transcription factors that maintain the expression of key genes and suppress others. (lonza.com)
  • Interestingly, we found that mating behavior was regulated independently by another sexual differentiation gene, Bmfru . (prolekarniky.cz)
  • Mutation experiments showed that this DR-1 site is essential for HNF4alpha transactivation of COUP-TFII. (lonza.com)
  • Most DEGs were involved in plant hormone signal transduction, plant-pathogen interaction and phenylalanine metabolism, and the genes significantly up-regulated in PI 114490 at 6 DPI were associated with defense response pathways. (frontiersin.org)
  • Expression of both COUP-TFI and COUP-TFII was significantly decreased in the cortisol-producing adenomas, in which CYP17 was drastically overexpressed, indicating that decreased expression of COUP-TFs play a key role in overexpression of CYP17 in this type of tumors. (elsevierpure.com)
  • Loss of Bmdsx gene expression significantly reduced the peripheral perception of the major pheromone component bombykol by reducing expression of the product of the BmOR1 gene which completely blocked courtship in adult males. (prolekarniky.cz)
  • We also identified two DFR genes (LOC101293749 and LOC101293459) that were significantly correlated with members in bHLH, MYB, WD40, AP2, and bZIP families. (biomedcentral.com)
  • Sexual back talk with evolutionary implications: stimulation of the Drosophila sex-determination gene sex-lethal by its target transformer. (prolekarniky.cz)
  • Here by using CRISPR/Cas9 and electrophysiological techniques we found that courtship and mating behaviors are regulated in male silk moths by mutating genes in the sex determination cascade belonging to two conserved pathways. (prolekarniky.cz)
  • Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)
  • In addition, we demonstrated that shRNA-mediated knockdown of Rasd1 results in further repression of Ear2-mediated renin transcription, whereas induction of Rasd1 by dexamethasone counteracts the effects of shRNA-mediated Rasd1 knockdown. (biomedcentral.com)
  • Finally, our study showed that Rasd1 missense mutations not only attenuate their physical interaction with Ear2 but also abolish their ability to counteract repression of renin transcription mediated by Ear2. (biomedcentral.com)
  • These results demonstrate that the Ov NRE contains not only sites responsible for the repression of the gene but also a positive element that is required for responsiveness to steroid hormones. (umn.edu)
  • The majority of the loss-of-function variants found in SOX17 were detected in the last exon of the gene. (bvsalud.org)
  • To further explore the molecular function of Rasd1, we performed a yeast two-hybrid screen and identified Ear2, a negative regulator of renin transcription, as an interaction partner of Rasd1. (biomedcentral.com)
  • Human NR2F1 genome location and NR2F1 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • Notably, COUP-TFI activated rather than repressed several target genes including the human CYP11B2 gene promoter, the results of which were opposite to those of the CYP17 promoter. (elsevierpure.com)
  • Further, the GR gene expresses a splicing variant GRbeta, which does not bind glucocorticoids but functions as a dominant negative isoform for GRalpha. (brainimmune.com)
  • The factors responsible for the postnatal and starvation-associated induction of HMGCS2 gene expression have been identified and shown to involve changes in circulating hormone and fatty acid levels. (aacrjournals.org)
  • Overview of all the structural information available in the PDB for UniProt: P24468 (COUP transcription factor 2) at the PDBe-KB. (wikipedia.org)
  • DFR and BZ1 structural genes and some bHLH, MYB, WD40, AP2, and bZIP TF family members enhance the accumulation of two pelargonidin derivatives. (biomedcentral.com)
  • In the dipteran Drosophila melanogaster , TGFbeta signaling stimulates JH production by up-regulating the transcription of the JH acid methyltransferase (JHAMT ) gene through the transcription factor Mothers against Dpp (Mad) 14 . (nature.com)
  • Mutations in the gene Dax-1 cause congenital adrenal hypoplasia. (nih.gov)
  • COUP-TFII plays a critical role in controlling the development of a number of tissues and organs including heart, blood vessels, muscles and limbs. (wikipedia.org)
  • 5. Hashiyama K, Hayashi Y, Kobayashi S. Drosophila Sex lethal gene initiates female development in germline progenitors. (prolekarniky.cz)
  • Further, they are the first to identify a gene that provides anterior-posterior axon guidance in a large brain nucleus and link Frizzled3 to corridor cell development. (jneurosci.org)
  • Genes and Development. (nycu.edu.tw)
  • Cardiac remodelling is a key risk factor for the development of heart failure in the chronic phase following myocardial infarction. (omeka.net)
  • Mutants in the corresponding genes initially failed to recapitulate the lymphatic defects observed in morphants. (bvsalud.org)
  • Transfection analyses of linker scanning mutants revealed a strong repressor site, designated the COUP- adjacent repressor (CAR) site, located between -119 and -111. (umn.edu)
  • Taken together, we identify the vicious cycle of inflammation, COUP-TFII, VEGF-C, and lymphangiogenesis in the endometriotic microenvironment, which opens up new horizons in understanding the pathophysiology of endometriosis. (endometriosistreatmentreport.com)
  • Further studies demonstrated that proinflammatory cytokines, via suppression of COUP-TFII level, induce VEGF-C overexpression. (endometriosistreatmentreport.com)
  • Finally, hCOUP-TFII overexpression showed that there is direct COUP-TFII autorepression as COUP-TFII occupies the proximal DR-1 binding site of its own gene in vivo. (lonza.com)
  • c-Myc transrepression is mediated by blockade of the transactivating activity of Miz-1, which occurs mainly through a Sp1-binding site in the proximal promoter of the gene. (aacrjournals.org)