• Therapeutic cloning, which creates embryonic stem cells . (medlineplus.gov)
  • The resulting embryo was then used to create embryonic stem cells, capable of generating every cell type in the body, and the nuclei of these cells were injected into other eggs to produce clones. (newscientist.com)
  • Therapeutic cloning produces embryonic stem cells for experiments aimed at creating tissues to replace injured or diseased tissues. (wisdomanswer.com)
  • Some people are very uneasy about creating a human embryo and then dismembering it, however early the stage, to obtain embryonic stem cells from which useful tissues might be grown. (globalchange.com)
  • XI - embryonic stem cells: embryonic cells that are capable of modifying the cells of any organism tissue. (hinxtongroup.org)
  • Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)
  • CytoSections are ready-to-use FFPE sections of cell pellets expressing targeted protein derived from gene-specific cDNA clones. (origene.com)
  • The following LCE1M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • 1. McKinnon D (1989) Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family. (aspetjournals.org)
  • Sequence of cDNA comprising the human pur gene and sequence-specificsingle-stranded-DNA-binding properties of the encoded protein. (embl-heidelberg.de)
  • We report here the cloning and sequencing of a cDNA encodinga protein with strong affinity for the PUR element. (embl-heidelberg.de)
  • We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
  • Organism cloning (also called reproductive cloning) refers to the procedure of creating a new multicellular organism, genetically identical to another. (wisdomanswer.com)
  • What's the difference between therapeutic cloning and reproductive cloning? (wisdomanswer.com)
  • Gene cloning, also known as DNA cloning, is a very different process from reproductive and therapeutic cloning. (wisdomanswer.com)
  • Since the term "born" has been used as an essential part of the definition of " reproductive cloning " used by Weissman, the National Academy of Sciences, etc., then it is critical to use the accurate term with the proper meaning. (lifeissues.net)
  • Otherwise, such a treaty would not recognize the inherent human nature of the early human embryo or fetus until after birth , and thus cloning them and using them for research - both "therapeutic" and "reproductive" -- would not be banned, and women undergoing "infertility treatments" could surely be put in danger. (lifeissues.net)
  • … "human clone" means an embryo that, as a result of the manipulation of human reproductive material or an in vitro embryo, contains a diploid set of chromosomes obtained from a single - living or deceased - human being, fetus, or embryo. (hinxtongroup.org)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • This now enables investigation of how parameters such as clonal expansion relate to gene expression phenotypes [ 28 ], thereby providing novel insights not accessible by bulk sequencing (e.g. (nature.com)
  • A critical portion of every cell or gene therapy study is the long-term follow-up (LTFU) observations to monitor the safety of therapeutic products. (genewiz.com)
  • Why is tissue culture asexual reproduction? (wisdomanswer.com)
  • Plant cloning, such as the use of plant tissue culture techniques, is an asexual reproduction of plants to produce duplicates from one plant specimen. (wisdomanswer.com)
  • Is cloning asexual reproduction? (wisdomanswer.com)
  • Clones are cells that have come from asexual reproduction or a parent cell. (wisdomanswer.com)
  • What's the difference between Clone reproduction and asexual reproduction? (wisdomanswer.com)
  • The key difference between asexual reproduction and clone reproduction is the setting of the process. (wisdomanswer.com)
  • The proteins arethe "work-horses" of the cells and are responsible for carrying out all the functions of the cell. (faqs.org)
  • α-crystallin is a member of the small heat shock protein family, which helps in maintaining and thickening of the cell wall and in providing stability to proteins that allow the bacteria to survive under harsher conditions 7 . (nature.com)
  • The most famous clone was a Scottish sheep named Dolly. (medlineplus.gov)
  • Indeed, if passed, Hatch/Feinstein/Kerry would explicitly legalize doing in humans the very cloning procedure -- somatic cell nuclear transfer (SCNT) -- that was used to make Dolly the sheep . (lifeissues.net)
  • Comment: Indeed, if passed, "total cloning bans" H.R. 534, H.R. 234, H.R. 916, and S. 245 would not ban anything either - not even the SCNT cloning technique that was used to make Dolly the sheep. (lifeissues.net)
  • Let's wind back the clock: these scientists had already carried out successful human nuclear transfer into an unfertilised egg before Dolly the sheep clone had been made. (globalchange.com)
  • But even they omitted to tell us anything until Dolly was seven months old, well over a year after the cloning technique was successfully carried out and a good two to three years perhaps after they began their secretive work. (globalchange.com)
  • Ian Wilmut, the cloning pioneer whose research was critical to the creation of Dolly the Sheep, has died, the Roslin Institute at the University of Edinburgh said Monday. (wgnradio.com)
  • LONDON (AP) - Ian Wilmut, the cloning pioneer whose work was critical to the creation of Dolly the Sheep in 1996, has died at age 79. (wgnradio.com)
  • Dolly also spurred questions about the potential cloning of humans and extinct species. (wgnradio.com)
  • He said the legacy of Wilmut's work in cloning Dolly continues to be seen. (wgnradio.com)
  • Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)
  • Cloning or clone reproduction is an in vitro process of obtaining multiple copies of the same organism using molecular biological and genetic engineering techniques. (wisdomanswer.com)
  • … "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)
  • Again, Saunders is referring to SCNT as "THE" cloning procedure, when there are many other ways to clone a human being as well, and he is scientifically mis-defining the product of SCNT (i.e., the cloned human embryo). (lifeissues.net)
  • secreted version: antibodies) and T cell receptor (TCR), respectively. (nature.com)
  • These complexes can be immunoextracted from cell lysatesusing monoclonal antibodies to either Pur alpha or Rb. (embl-heidelberg.de)
  • Potentially easier would be cloning cryogenically frozen humans, though the consensus among cloning experts is that it would be unethical and dangerous to clone a human. (newscientist.com)
  • Monkeys and humans have 97% of genes in common so if the right 1.6% were transferred from a human to a monkey we could land up with a monkey more human than animal. (globalchange.com)
  • 5. Chandy KG, Williams CB, Spencer RH, Aguilar BA, Ghanshani S, Tempel BL, and Gutman GA (1990) A family of three mouse potassium channel genes with intronless coding regions. (aspetjournals.org)
  • 9. Grissmer S, Dethlefs B, Wasmuth JJ, Goldin AL, Gutman GA, Cahalan MD, and Chandy KG (1990) Expression and chromosomal localization of a lymphocyte K + channel gene. (aspetjournals.org)
  • GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
  • Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells. (genscript.com)
  • ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
  • 4. Yokoyama S, Imoto K, Kawamura T, Higashida H, Iwabe N, Miyata T, and Numa S (1989) Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells: primary structure and functional expression from cDNAs. (aspetjournals.org)
  • 7. Roberds SL and Tamkun MM (1991) Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart. (aspetjournals.org)
  • 8. Paulmichl M, Nasmith P, Hellmiss R, Reed K, Boyle WA, Nerbonne JM, Peralta EG, and Clapham DE (1991) Cloning and expression of a rat cardiac delayed rectifier potassium channel. (aspetjournals.org)
  • As the dominant viral delivery vector for approved cell therapies, lentivirus requires ISA in pre-clinical and clinical trials due to its possibility of altering expression of cellular genes which could contribute to tumorigenicity. (genewiz.com)
  • The Drosophila wing primordium is defined by expression of the selector gene vestigial ( vg ) in a discrete subpopulation of cells within the wing imaginal disc. (biologists.com)
  • Short-range DSL (Delta/Serrate/LAG-2)-Notch signaling between D and V cells drives vg expression in `border' cells that flank the boundary. (biologists.com)
  • It also induces these same cells to secrete the long-range morphogen Wingless(Wg), which drives vg expression in surrounding cells up to 25-30 cell diameters away. (biologists.com)
  • All of these selector genes are initially activated in small, discrete groups of founder cells, and their states of expression, whether `on' or `off', are then stably inherited in descendent cells that constitute the compartment. (biologists.com)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)
  • The data presented is normalized to the expression of Rpl32 , a housekeeping gene. (cdc.gov)
  • 7. "[footnote 16]: The cloning procedure supplies the oocyte with a complete set of chromosomes, all of which are contained in the nucleus which is transferred into the denucleated oocyte. (lifeissues.net)
  • It's given name is the "Human Cloning Ban and Stem Cell Research Protection Act of 2003," the stated purpose of which, supposedly, is to "prohibit human cloning and to protect important areas of medical research, including stem cell research. (lifeissues.net)
  • This paper gives an Islamic perspective on some of these advances, including abortion, in vitro fertilization, genetic engineering, cloning and stem cell research. (who.int)
  • If the recipes have extra bases or misspelled bases or if some are deleted, the cell can make a wrong protein or too much or too little of the right one. (faqs.org)
  • The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. (origene.com)
  • The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. (origene.com)
  • Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
  • The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1cells complexed with Pur alpha, a sequence-specific single-strandedDNA-binding protein implicated in control of gene transcription and DNAreplication. (embl-heidelberg.de)
  • One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence. (genscript.com)
  • The sequence acts, therefore, as an "instruction manual" that directs all cell functions. (faqs.org)
  • DNA in the cells of the islets of Langerhans in the pancreas would normally contain that base sequence since the islets are the regions in which insulin is produced in mammals. (faqs.org)
  • The human Pur factor binds strongly to a sequence element repeated withinzones of initiation of DNA replication in several eukaryotic cells. (embl-heidelberg.de)
  • Perhaps Ramsey would give other extraordinarily powerful arguments as to why human cloning is unethical, but he obviously would not be able to base it on his unscientific "pre-embryo" position. (lifeissues.net)
  • We present molecular and epidemiologic evidence that the emergence of the USA300 clone has led to not only an increase in CA-MRSA infections but also an overall increase in SSTIs in our patient population. (cdc.gov)
  • All animals are composed of distinct body parts and organs, many of which are specified by particular combinations of `selector' genes that control cell and tissue behavior by regulating downstream `realisator' genes( Garcia-Bellido, 1975 ). (biologists.com)
  • Recent advances in single-cell sequencing enable recovery of the complete adaptive immune receptor sequences in addition to transcriptional information. (nature.com)
  • The gene is localized on the same chromosome at the distance of about 50 kb from telomeric sequences of IL-17A gene, and both genes are in a tail-to-tail orientation [ 3 ]. (hindawi.com)
  • Cow / human clone hybrid - cow and human mixed together. (globalchange.com)
  • The world's first human clone of an adult has now been made, by an American biotechnology company in Massachusetts, Advanced Cell Technology. (globalchange.com)
  • Technically 1% of the human clone genes would have belonged to the cow - the mitochondria genes. (globalchange.com)
  • What happens when a clone is taken from an adult? (wisdomanswer.com)
  • As a consequence, clones created from a cell taken from an adult might have chromosomes that are already shorter than normal, which may condemn the clones' cells to a shorter life span. (wisdomanswer.com)
  • Wilmut set off a global discussion about the ethics of cloning when he announced that his team at the university's Roslin Institute for animal biosciences had cloned a lamb using the nucleus of a cell from an adult sheep. (wgnradio.com)
  • The lamb's cloning was the first time scientists were able to coax a mature adult cell into behaving like a cell from a newly fertilized embryo in order to create a genetically identical animal. (wgnradio.com)
  • A) Sex differences in density of -gal+ (PR+) cells in adult PRPL/PL mice. (cdc.gov)
  • Healthy mice have been cloned from cells from dead mice that had been frozen for 16 years, raising the possibility that endangered species could be cloned from old carcasses that have been tossed in freezers, rather than from living cells frozen using elaborate techniques. (newscientist.com)
  • Other teams have already cloned mice from previously frozen dead cells . (newscientist.com)
  • F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)
  • G) No sex difference in nuclear or soma size of PR+ VMHvl cells in PRPL mice. (cdc.gov)
  • The Wakayama team used a modified version of a cloning technique in which the nucleus of a mouse cell - in this case a cell from dead tissue that has been frozen and then thawed - is injected into a mouse egg that has had its nucleus removed. (newscientist.com)
  • Wakayama speculates that freezing and thawing the tissue somehow makes it easier to "reprogram" the brain cell nucleus. (newscientist.com)
  • 2. Kamb A, Weir M, Rudy B, Varmus H, and Kenyon C (1989) Identification of genes from pattern formation, tyrosine kinase, and potassium channel families by DNA amplification. (aspetjournals.org)
  • Together, this dataset provides a resource for immunologists that can be integrated with future single-cell immune repertoire and transcriptome sequencing datasets. (nature.com)
  • new nomenclature, gene organization, and divergence of human and rodent genes. (genscript.com)
  • This copy can be obtained from a natural source (like DNA in islets of Langerhans cells), or it can be manufactured artificially in the laboratory. (faqs.org)
  • The technique of plant tissue culture involves micropropagation and plant cell culture. (wisdomanswer.com)
  • Following are the various advantages of tissue culture technique: The plantlets are obtained in a very short time with a small amount of plant tissue. (wisdomanswer.com)
  • The technique required three elements: the gene to be transferred, a host cell in which the gene is to be inserted, and a vector for transferring the gene to the body. (faqs.org)
  • R.I. FRESHNEY: "Culture of Animal Cells: A Manual of Basic Technique", 1987, ALAN R. LISS, INC. (sumobrain.com)
  • Besides, an increase in T cell responses are observed against HspX in healthy latent individuals as compared to active TB patients suggesting its role in maintaining a disease free state in these subjects 9 . (nature.com)
  • Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. (origene.com)
  • Brain tissue is also high in sugars, which can protect cells when they freeze. (newscientist.com)
  • Many zoos are not in a position to collect cells and freeze them in such a way as to preserve their viability, says Robert Lanza of Advanced Cell Technology in Worcester, Massachusetts, but they can put a dead animal "in a plastic bag and throw it in the freezer", he adds. (newscientist.com)
  • His purpose he said was to freeze clones to be used later for spare parts. (globalchange.com)
  • They took a cell from Dr Jose Cibelli, a research scientist and combined it with a cows egg from which the genes had already been removed. (globalchange.com)
  • Elsewhere on this site I describe my own conversations with a British scientist in the 1980s who was attempting then to clone human embryos - with some success. (globalchange.com)
  • Suppose, for example, that one wishes to insert the insulin gene into a bacterial cell. (faqs.org)
  • In essence this form of cloning is an asexual method of reproduction, where fertilization or inter-gamete contact does not take place. (wisdomanswer.com)
  • CA-MRSA infections are predominantly skin and soft tissue infections (SSTIs), are often susceptible to other non-β-lactam antimicrobial drugs, and carry a type IV or V staphylococcal cassette chromosome (SCC) with the mec A gene. (cdc.gov)
  • Adaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past and current immune responses. (nature.com)
  • The lack of targeted immunotherapeutic approaches for T cell malignancies results in part from potential risks associated with targeting broadly expressed T cell markers, namely T cell depletion and clinically significant immune compromise. (mdpi.com)
  • But it is perhaps not auspicious to quote him for purposes of the scientific debates on human cloning, because Ramsey agreed with and supported the scientific myth of the "pre-embryo" 47 made famous by Jesuit Richard McCormick and frog embryologist Clifford Grobstein. (lifeissues.net)
  • You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart. (genscript.com)
  • The second step is to insert the insulin gene into the vector.Viruses, liposomes (hollow spheres of fat molecules formed in solution), andplasmids (circular forms of DNA) are common vectors. (faqs.org)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • The year after Dolly's creation, U.S. President Bill Clinton imposed a ban on the use of federal funds for human cloning but stopped short of banning all cloning research. (wgnradio.com)
  • His work was critical to research that aims to treat genetic and degenerative diseases by helping the body repair damaged tissue. (wgnradio.com)
  • In the fast 40 years, amazingdiscoveries and development of revolutionary new techniques have allowed scientists to learn a great deal about how genes work and how they are linked todisease. (faqs.org)
  • Wilmut, a trained embryologist, later focused on using cloning techniques to make stem cells that could be used in regenerative medicine. (wgnradio.com)
  • Genetic engineering is the altering of the genetic material of living cells in order to make them capable of producing new substances or performing new functions. (faqs.org)
  • Here, we recovered transcriptome and immune repertoire information for polyclonal T follicular helper cells following lymphocytic choriomeningitis virus (LCMV) infection, CD8+ T cells with binding specificity restricted to two distinct LCMV peptides, and B and T cells isolated from the nervous system in the context of experimental autoimmune encephalomyelitis. (nature.com)
  • Dolly's creation prompted other scientists to clone animals including dogs, cats, horses and bulls. (wgnradio.com)
  • In recent years, scientists have proposed bringing back the woolly mammoth by using a mix of gene editing and cloning. (wgnradio.com)
  • But this is the first time animals have been cloned from lumps of tissue frozen without the use of chemicals that might protect the cells from damage. (newscientist.com)
  • These programs depend on the animal cells undergoing specialised chemical procedures before being frozen so that they come to life when thawed. (newscientist.com)
  • Practical considerations such as the necessary cost and time required to generate and analyze each single-cell sequencing sample has been a limitation to performing large-scale experiments (i.e., with many samples). (nature.com)
  • By using the tissue culture process, a plant's yield can be increased dramatically, and in a short amount of time. (wisdomanswer.com)
  • A conditional knockout resource for the genome-wide study of mouse gene function. (genscript.com)
  • Cloning is an important process in Recombinant DNA technology. (wisdomanswer.com)
  • IL-17F is expressed in activated CD4 + T cells, basophils, and mast cells, three important cell types involved in allergic airway inflammation [ 3 ]. (hindawi.com)