• Although aerobic respiration may seem a more energetically-efficient pathway to grow on glucose, it is in fact a rather inefficient way to increase biomass as most of the carbon in the glucose is oxidized to carbon dioxide rather than incorporated into new amino acids or fatty acids. (wikipedia.org)
  • Eu3 encodes UreG, a GTP'ase necessary for proper emplacement of Ni and carbon dioxide in the urease active site. (usda.gov)
  • These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. (nih.gov)
  • 2. A bacterial enzyme controls the entire production of carbon dioxide and ammonia from urea. (beckersasc.com)
  • As these fuels would be produced from bacteria fed with plant matter - which is made from carbon dioxide pulled from the atmosphere - burning them in engines will significantly reduce the amount of added greenhouse gas relative to any fuel generated from petroleum. (scienceblog.com)
  • In a global sense, what's happening in the ocean that makes it so different from what's happening on land is that most of the carbon that's being fixed every single day is being turned back into carbon dioxide by heterotrophic cells. (asm.org)
  • Meanwhile, burning carbon compounds produces gases like carbon dioxide. (businessinsider.com)
  • US researchers have genetically modified bacteria to eat carbon dioxide and produce isobutyraldehyde - a precursor to several useful chemicals, including isobutanol, which has great potential as a fuel alternative to petrol. (chemistryworld.com)
  • To understand more about this key point in evolution, evolutionary biologist Betül Kacar at Harvard University decided to reconstruct the ancient form of rubisco, the key enzyme in photosynthesis that converts carbon dioxide into the precursors of sugars. (newscientist.com)
  • The modern rubisco molecule has to be selective because it encounters both oxygen and carbon dioxide, but even so it sometimes goes after the wrong gas . (newscientist.com)
  • It will have the capabilities of sensing and mineralizing the toluene into carbon dioxide and water, but this terminator will not be back. (igem.org)
  • This reaction proceeds to near 100% completion due to the release of the product carbon dioxide and can be used for the synthesis of a range of sugars of interest to the pharmaceutical industry. (rcsb.org)
  • He welded on low-carbon mild steel using Metal Inert Gas (MIG) with solid or flux core wire and 75% argon/25% carbon dioxide shield gas. (cdc.gov)
  • Yet life as we know it is organic, or based on carbon - from DNA to proteins to how it stores and uses energy. (businessinsider.com)
  • The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
  • In addition to the host regulatory mechanisms response to disease, the microbiome can make changes to the expression of the host features such as their genes, proteins, and/or PTMs. (rsc.org)
  • Pure culture AOB (Nitrosmonas europaea and Nitrosomonas eutropha) and mixed culture nitrifying consortia were cultivated in bioreactors and continuously exposed to methane to study the responses of nitrifying bacteria at reactor level (nitrification performance) and molecular level (genes and proteins expression). (columbia.edu)
  • Using a soil bacterium called Bacillus subtilis , Dr Stanley-Wall has investigated the genes and proteins required for biofilms to develop. (sciencedaily.com)
  • Furthermore, a huge number of genes have an unknown function and no homology to known proteins from other bacteria. (uni-goettingen.de)
  • All 6 ADHs are expressed, and Cphy3925 and Cphy1029 are among the most highly expressed proteins on all tested carbon sources (19, 39). (researchgate.net)
  • Whereas regulating gene expression in multicellular organisms allows for cellular differentiation, in single-celled organisms like prokaryotes, it primarily ensures that a cell's resources are not wasted making proteins that the cell does not need at that time. (pressbooks.pub)
  • In bacteria and archaea , structural proteins with related functions are usually encoded together within the genome in a block called an operon and are transcribed together under the control of a single promoter , resulting in the formation of a polycistronic transcript ( Figure 12.30 ). (pressbooks.pub)
  • In addition, the assembled CM-01 genome was successfully annotated against the Cluster of Orthologous Groups of proteins database, Gene Ontology database, and Kyoto Encyclopedia of Genes and Genome pathway database. (bvsalud.org)
  • RafK carries a C-terminal regulatory domain present in a subset of ATP-binding proteins that has been involved in both direct regulation of transporter activity (inducer exclusion) and transcription of transporter genes. (lu.se)
  • Studies of a pckA-lacZ gene fusion indicated that when cells were grown in minimal media with various carbon sources, such as succinate, malate, pyruvate, lactate, or ethanol, under both anaerobic light and aerobic dark conditions, the pckA gene was induced in log phase, irrespective of the carbon source. (asm.org)
  • Hydrogenases are well-known for their roles in anaerobic bacteria, but this is the first comprehensive study of these enzymes in an organism that requires oxygen to combust their fuel sources. (science20.com)
  • Anaerobic bacteria and fungi are also prevalent. (who.int)
  • The causative bacteria may be aerobic, anaerobic, or mixed flora. (medscape.com)
  • The new genomic resources available including the soybean genome sequence and access to a new screening technology enabled the localization and identification of the mutant gene, an accessory protein similar to one that exists in bacteria. (usda.gov)
  • The soybean genome contains a single gene encoding UreG/Eu3, and two paralogs each for UreD and UreF. (usda.gov)
  • The human genome doesn't include the gene for urease. (beckersasc.com)
  • Liao's team modified the genome of the cyanobacteria Synechococcus elongatus by incorporating four genes from other bacteria into the structure. (chemistryworld.com)
  • A novel transketolase has been reconstituted from two separate polypeptide chains encoded by a 'split-gene' identified in the genome of the hyperthermophilic bacterium, Carboxydothermus hydrogenoformans . (rcsb.org)
  • Annotation of the genome identified 5538 protein-coding genes, 3 rRNA genes, 54 tRNAs, and no plasmids were found. (bvsalud.org)
  • Besides these, 39 interspersed repeat and 141 tandem repeat sequences, 6 prophages, 51 genomic islands, 94 insertion sequences, 4 clustered regularly interspaced short palindromic repeats, 5 antibiotic-resistant genes, and 150 virulence genes were also predicted in the P. koreensis CM-01 genome. (bvsalud.org)
  • In recent, the occurrence of Gram-negative multidrug-resistant pathogens which are resistant to many available antibiotics has revived its clinical application in healthcare centers, since colistin is broadly considered as one of the last-line options of antibiotic therapy for multidrug-resistant bacteria. (hindawi.com)
  • The fates of viruses, bacteria, and antibiotic resistance genes during advanced wastewater treatment are important to assess for implementation of potable reuse systems. (stanford.edu)
  • Concentrations of bacteria (16S rRNA gene), viruses (human adenovirus and JC polyomavirus), and antibiotic resistance genes (sul1 and bla TEM ) were assessed via quantitative PCR following the concentration of large sample volumes by dead-end ultrafiltration. (stanford.edu)
  • The increasing prevalence of antibiotic-resistant uropathogenic E. coli (UPEC) together with the recognition of UPEC as intracellular bacteria confront our current treatment regimens of urinary tract infections. (oru.se)
  • Intracellular bacteria that are protected from the host inflammatory response and antibiotic treatment may later exit the host cells and re-infect the urinary tract which enhances the risk of recurrent urinary tract infections. (oru.se)
  • Traditional urban wastewater treatment plants (UWTPs) are deficiently efficient in eliminating most contaminants of emerging concern (CECs), comprising antibiotics, antibiotic resistant bacteria and antibiotic resistance genes (ARB & ARGs). (scirp.org)
  • however, the lateral gene transfer and diffusion of antibiotic resistance genes (ARGs) [11], may be promoted in the UWTPs, due to the elevated microbial density and supplementary selection pressures [12]. (scirp.org)
  • Targeting at the microbial demobilization, disinfecting wastewater can constitute a chance to restrict the liberation of antibiotic-resistant bacteria (ARB) into nature and play a part in the reduction of the environmentally-related danger of diffusing resistance determinants. (scirp.org)
  • Evidence for wastewaters as environments where mobile antibiotic resistance genes emerge. (janusinfo.se)
  • An effective profenofos and λ-cyhalothrin degrading bacterium (strain DB17) was isolated from soil samples collected from Qassim region, Saudi Arabia. (omicsonline.org)
  • The surviving bacteria were a parade of microbes that took turns dominating the burned soil. (earth.com)
  • Aiming to exploit the genetic potential of soils microorganisms from extreme environments to retrieve salinity tolerance genes, the diversity of saline soil was examined and soil metagenomic DNA was used. (conicet.gov.ar)
  • Metagenomic DNA from both soil samples was extracted and used to amplify the 16S rRNA gene by using primers deduced from the division bacteria. (conicet.gov.ar)
  • Here, we investigated the effects of extreme drought and exotic plants, two of the most significant threats to Mediterranean-type ecosystems, on soil microbial community composition and carbon metabolic genes within a four-year field rainfall manipulation experiment. (nature.com)
  • We combined measurements of bulk microbial and soil properties with high-throughput microbial community analyses to elucidate microbial responses and microbial-mediated alterations to carbon cycling. (nature.com)
  • Relationships found between microbial taxonomic groups and carbon metabolic genes support the interpretation that exotic plants change microbial carbon cycling by altering the soil microclimate and supplying easily decomposed high-quality litter. (nature.com)
  • Researchers at New Zealand's University of Otago have found that the soil bacterium Mycobacterium smegmatis uses two special enzymes that can efficiently scavenge hydrogen as fuel at very low concentrations. (science20.com)
  • It doesn't require fertile soil or lots of space either-in fact, the bacteria don't need land at all. (stlmag.com)
  • SAR11 and SAR202 are the poles on the spectrum of heterotrophic marine bacteria. (asm.org)
  • Heterotrophic bacteria had a mean value of 7.4x10 5 cell.ml -1 . (usp.br)
  • Heterotrophic bacteria were abundant in coastal areas probably due to increased of organic matter, which corroborates the result of higher biomass carbon in coastal region. (usp.br)
  • Pulse-labeling and nanoSIMS analysis showed a physiological coupling of the phototrophic and heterotrophic partner by exchange of carbon and nitrogen compounds. (dsmz.de)
  • The beneficial effect of heterotrophic bacteria on the growth of algae and cyanobacteria has been observed by phycologists decades ago. (dsmz.de)
  • Furthermore, DGGE (Denaturing Gradient Gel Electrophoresis) technique was used to separate the products of a nested amplification (the variable region of 16S rRNA gene) using bacterial primers, and primers specific for the genera Burkholderia. (conicet.gov.ar)
  • Sticky nanotubes that trap bacteria like flypaper can be used to identify bacterial infections in seconds rather than days, report Spanish chemists. (chemistryworld.com)
  • Although only tested on the typhus-causing Salmonella typhi bacteria so far, if the process can be applied more widely it could revolutionise bacterial testing in the medical and food industries. (chemistryworld.com)
  • Acclimation of the mixed culture nitrifying consortia to co-exposure and co-oxidation of ammonia and methane were shown at multiple levels (reactor performance, biomass concentrations, bacterial activities, and genes expression). (columbia.edu)
  • However, much is unknown about the effects of indigenous bacteria on microalgal growth and the characteristics of bacterial communities associated with microalgae in microalgae-effluent culture. (biomedcentral.com)
  • Furthermore, the total numbers of bacterial 16S rRNA genes in the 7-day microalgae-effluent cultures were 109‒793 times the initial numbers. (biomedcentral.com)
  • These results suggest that the three microalgae produced and supplied organic carbon that supported bacterial growth in the effluent. (biomedcentral.com)
  • To enhance their own growth, microalgae may be able to selectively stimulate specific bacterial groups from among the in situ indigenous bacterial community found in wastewater effluent (i.e., microalgae growth-promoting bacteria: MGPB). (biomedcentral.com)
  • Diauxie occurs because organisms use operons or multiple sets of genes to control differently the expression of enzymes needed to metabolize the different nutrients or sugars they encounter. (wikipedia.org)
  • Through evolution, organisms have developed the ability to regulate their genetic control mechanisms so as to only express those genes resulting in the fastest growth rate. (wikipedia.org)
  • People suffer an illness and take antibiotics which destroys bacteria and new organisms appear. (earth.com)
  • Purple nonsulfur bacteria (PNSB) are metabolically versatile organisms which are able to grow either aerobically in the dark or anaerobically in the light using hydrogen or organic compounds as electron donors and subsequently fixing CO 2 ( 16 ). (asm.org)
  • Kacar and her team compared rubisco gene sequences from modern organisms to infer what the sequence must have been in their common ancestor . (newscientist.com)
  • The particles look like one-celled organisms and are about 4 to 10 thousandths of a millimeter wide, somewhat larger than typical bacteria. (zetatalk.com)
  • Bacteria are single cell organisms with no nervous system or brain. (sciencedaily.com)
  • This means that bacteria scavenging this highly dependable fuel source would be especially competitive against other organisms in their volatile environments. (science20.com)
  • One enzyme important in nitrogen movement is activated urease, a nickel containing protein that has been conserved from bacteria through the plant kingdom. (usda.gov)
  • 1.83% organic carbon, 0.18% organic nitrogen, and CH2: elec. (conicet.gov.ar)
  • of key genes for nitrogen and carbon fixation, sulfate decrease, Dovitinib novel inhibtior sulfide oxidation and fermentation. (columbiagypsy.net)
  • The requirement of external carbon for conventional biological nitrogen removal (BNR) process has stimulated research on more resource-efficient treatment technologies. (columbia.edu)
  • Furthermore, the accumulation of nitrite and a more substantive impact on nitrite oxidizing bacteria growth (compared to AOB) indicated the possibility for partial nitrification (ammonia-N to nitrite-N) coupled with biomethanol production, thereby opening the prospect for even more resource-efficient concurrent carbon and nitrogen management and removal. (columbia.edu)
  • Such hot composting requires a volume of a cubic yard or more of waste, assembled all at one time, with a good mix of materials that provide an appropriate ratio of carbon to nitrogen. (eugeneweekly.com)
  • Mix materials for a carbon to nitrogen ratio near 25:1 (see below). (eugeneweekly.com)
  • Recipes for mixes to achieve ideal carbon-nitrogen ratios are posted at http://wkly.ws/11s Look for "Gardening Links" and click on "Composting Information. (eugeneweekly.com)
  • They don't want to fall into lab-speak about nitrogen fixation and gene regulation in filamentous cyanobacteria-or blue-green algae, to the layperson. (stlmag.com)
  • She works with a process called nitrogen fixation, in which the bacteria convert nitrogen into ammonia, creating hydrogen as a byproduct. (stlmag.com)
  • Unlike corn-based ethanol, for example, nitrogen fixation doesn't produce carbon emissions or affect food prices. (stlmag.com)
  • The researchers continued to test whether fungi and bacteria could thrive at different points in time because of their unique and varied traits. (earth.com)
  • This research shows fungi and bacteria may have developed similar coping strategies. (earth.com)
  • Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)
  • Kacar's team has now synthesised the gene sequences to make the ancient rubisco and is using CRISPR gene-editing technology to insert them into cyanobacteria. (newscientist.com)
  • It is unclear whether sul1 in the RO permeate was from the passage of resistance genes or new growth of microorganisms, but the concentrations were on the low end of those reported for conventional drinking water distribution systems. (stanford.edu)
  • There is concern, however, that these non-native microorganisms may upset the balance of the ecosystem through unnatural competition or horizontal synthetic gene transfer. (igem.org)
  • Herbivores rely on digestive tract lignocellulolytic microorganisms, including bacteria, fungi and protozoa, to derive energy and carbon from plant cell wall polysaccharides. (concordia.ca)
  • Em relação aos domínios, a distribuição de Bacteria ocorreu na superfície e de Archaea no fundo da região oceânica. (usp.br)
  • Using real time PCR were analysed 16S abundance of bacteria, archaea and SAR11 clade and the expression of the rubisco and proteorhodopsin gene. (usp.br)
  • In relation to the domains, the distribution of bacteria occurred in the surface and the Archaea in the deep of the ocean region. (usp.br)
  • iPHoP: A Matchmaker for Phages and their Hosts Building on existing virus-host prediction approaches, a new tool combines and evaluates multiple predictions to reliably match viruses with their archaea and bacteria hosts. (doe.gov)
  • Proteomics and gene expression data from pure culture experiments suggested that AOB modulating their catabolic (energy synthesis) and anabolic (biomass synthesis) pathways in response to methane exposure. (columbia.edu)
  • SAR11 is like a house with the lights on all the time, in that the cells constitutively express most metabolic genes. (asm.org)
  • There is also evidence of autotrophic bacteria capable of synthesising organic C (OC) by the reduction of CO 2 in the marine environment 18 . (nature.com)
  • He had scoured the scientific literature for organic compounds with three-carbon rings and found just two known examples, both made by Streptomyces bacteria that are nearly impossible to grow in a lab environment. (scienceblog.com)
  • Comparative transcriptomic analysis of methane cometabolism in N. europaea and N. eutropha showed that AOB upregulated genes involved in ATP production while downregulated genes involved in NADH production, organic molecules synthesis and cell division. (columbia.edu)
  • Biogeochemical data indicated that the decomposition of native organic matter occurred in different phases, beginning with mineralization of dissolved organic matter (DOM) to CO 2 during the first week of incubation, followed by a pulse of acetogenesis that dominated carbon flux after 2 weeks. (frontiersin.org)
  • Diauxic growth, meaning double growth, is caused by the presence of two sugars on a culture growth media, one of which is easier for the target bacterium to metabolize. (wikipedia.org)
  • While growing these bacteria on various combination of sugars during his doctoral thesis research, Monod observed that often two distinct growth phases are clearly visible in batch culture, as seen in Figure 1. (wikipedia.org)
  • While we rely on carbon sources such as sugars and amino acids, many bacteria can use gases (e.g. hydrogen, carbon monoxide) and even metals (e.g. iron, uranium) as fuel sources for growth and survival," says Mr Greening. (science20.com)
  • Soils under exotic plants had increased temperatures, enhanced infiltration during rainfall events, and decreased water retention and labile carbon in comparison to soils under native plants. (nature.com)
  • The hierarchy of controls can be used to prevent workplace exposure to welding fumes and gases, and soils that may be contaminated with B. cereus group bacteria producing anthrax toxins. (cdc.gov)
  • In this review, we discuss considerations of the study design for each data feature, the limitations in gene and protein abundance and their rate of expression, the current data integration methods, and microbiome influences on gene and protein expression. (rsc.org)
  • Interspecies associations Just the shallow aquifer got a very clear succession with a solid adverse correlation between your genera and (was within nearly every sample and dominated the machine with up to 83% out of all the total 16S rRNA gene reads, whereas was either Dovitinib novel inhibtior undetectable or in low abundance (Figure 5). (columbiagypsy.net)
  • Quantitative polymerase chain reaction validation of three complete vMAGs revealed their independence from host bacterium abundance. (microbial-ecology.net)
  • Isolation of indigenous bacteria capable of degrading pesticides provides an environmentally friendly way for pesticide detoxification [ 2 ]. (omicsonline.org)
  • Microalgal productivity could be enhanced if the positive effects of indigenous bacteria could be exploited. (biomedcentral.com)
  • To assess the effects of the indigenous bacteria in wastewater effluent on microalgal growth, three microalgae, Chlamydomonas reinhardtii , Chlorella vulgaris , and Euglena gracilis , were cultured in two municipal wastewater effluents and one swine wastewater effluent with and without indigenous bacteria for 7 days. (biomedcentral.com)
  • All microalgae grew better in all effluents with indigenous bacteria than without bacteria. (biomedcentral.com)
  • 5, 2023 Bacteria are also able to communicate with each other through chemical signals, a behavior known as quorum signaling (QS). (sciencedaily.com)
  • Concentrations of 16S rRNA and sul1 genes were reduced by treatment but remained quantifiable in reverse osmosis permeate. (stanford.edu)
  • Importantly, the technique is very precise - able to detect concentrations as low as a single bacterium in 5 millilitres of solution. (chemistryworld.com)
  • The idea of enzyme adaptation was thus replaced with the concept of enzyme induction, in which a molecule induces expression of a gene or operon, often by binding to a repressor protein and preventing it from attaching to the operator. (wikipedia.org)
  • Well aware of the advantages biology has to offer, a group of biofuel experts led by Lawrence Berkeley National Laboratory (Berkeley Lab) took inspiration from an extraordinary antifungal molecule made by Streptomyces bacteria to develop a totally new type of fuel that has projected energy density greater than the most advanced heavy-duty fuels used today, including the rocket fuels used by NASA. (scienceblog.com)
  • Keasling's team, comprised of JBEI and ABPDU scientists, studied the genes from the original strain ( S. roseoverticillatus) that encode the jawsamycin-building enzymes and took a deep dive into the genomes of related Streptomyces, looking for a combination of enzymes that could make a molecule with jawsamycin's toothy rings while skipping the other parts of the structure. (scienceblog.com)
  • Silicon generally doesn't form unique versions of the same molecule, but carbon often does - and that chemical diversity gives life a lot of room to play. (businessinsider.com)
  • The modified bacteria, they hope, will then produce a form of rubisco molecule not seen on Earth for billions of years. (newscientist.com)
  • Chemical tests indicated they also lacked DNA, the gene-carrying molecule that most types of cells contain. (zetatalk.com)
  • The most abundant organism on Earth lives in its seas: the marine bacterium SAR11. (asm.org)
  • Foram realizadas análises de abundância do gene 16S de bactérias, archaeas e do clado Sar11 e a expressão dos genes rubisco e proteorodpsina por meio de PCR em tempo real. (usp.br)
  • Monod later put aside his work on diauxic growth and focused on the lac operon model of gene expression, which led to a Nobel prize. (wikipedia.org)
  • cAMP, in turn, is required for the catabolite activator protein (CAP) to bind to DNA and activate the transcription of the lac operon, which includes genes necessary for lactose metabolism. (wikipedia.org)
  • For example, in E. coli , all of the structural genes that encode enzymes needed to use lactose as an energy source lie next to each other in the lactose (or lac ) operon under the control of a single promoter, the lac promoter. (pressbooks.pub)
  • Pneumococci lacking RafK showed a 50- to 80-fold reduction in expression of the raf operon genes aga (alpha-galactosidase) and rafEFG (raffinose substrate binding and permease genes), and both glucose and sucrose inhibited raffinose uptake through inducer exclusion. (lu.se)
  • Like RafK, the presence of DLDH also activated the expression of raf operon genes, as DLDH-negative pneumococci showed a significantly decreased expression of aga and rafEFG, but DLDH did not regulate rafK or the putative regulatory genes rafR and rafS. (lu.se)
  • Polymerase chain reaction (PCR) primers were designed based on the LAF_1318 sequences and the corresponding FAE gene was amplified from L. fermentum NRRL B-1932 and subcloned into an expression vector pET28b (Novagen) at the NcoI/BamHI sites. (usda.gov)
  • Based on the results of phylogenetic similarity of 16SrDNA gene sequences, strain DB17 was identified to be Pseudomonas putida. (omicsonline.org)
  • Although applicant phylum OD1 got minimal diversity (nine OTUs) in borehole KF0069A01, the 16S rRNA gene sequences constituted exclusive OTUs not bought at the additional sampling sites. (columbiagypsy.net)
  • Study first author Fabiola Pulido-Chavez noticed that genes involved in methane metabolism doubled in post-fire microbes. (earth.com)
  • These genes, which come from L. lactis , B. subtilis and E. coli , produce enzymes that hijack the metabolism of the microbes - turning them into miniature reaction vessels. (chemistryworld.com)
  • A) Central carbon metabolism in M. pneumoniae. (uni-goettingen.de)
  • The depletion of DOM over time was strongly correlated with increases in expression of many genes associated with heterotrophy (e.g., amino acid, fatty acid, and carbohydrate metabolism) belonging to a Hydrogenophaga strain that accounted for a relatively large percentage (~8%) of the metatranscriptome. (frontiersin.org)
  • a protein found in the blood that is produced in response to foreign substances (e.g., bacteria or viruses) invading the body. (cdc.gov)
  • 4.Flint HJ, Bayer EA, Rincon MT, Lamed R, White BA (2008) Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis. (concordia.ca)
  • Symbiosis genes" of the epibiont were identified by genomic approaches. (dsmz.de)
  • Sincle cell genomic analysis of the bacteria constituting different types of phototrophic consortia indicate a long-term co-evolution of the partners. (dsmz.de)
  • Genomic DNA contains both structural gene s, which encode products that serve as cellular structures or enzymes, and regulatory gene s, which encode products that regulate gene expression. (pressbooks.pub)
  • The use of agrifood wastes that replace engineered substrates as biostimulants permits the low carbon impact of bioremediation treatment as part of a circular economy approach. (mdpi.com)
  • In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikidoc.org)
  • Sequencing of a 2.5-kb chromosomal Sma I- Pst I fragment containing the structural gene revealed an open reading frame encoding 537 amino acids, homologous to known pckA genes. (asm.org)
  • To understand how severe wildfires affect bacteria and fungi, mycologist Sydney Glassman led a team of researchers into the burn scar of the Holy Fire, which burned 23,000 acres in 2018. (earth.com)
  • 1. Dr. Hecht says although the existence of gut bacteria was largely known, researchers have only recently begun to understand their significance. (beckersasc.com)
  • Supercharging SIP in the Fungal Hyphosphere Applying high-throughput stable isotope probing to the study of a particular fungi, researchers identified novel interactions between bacteria and the fungi. (doe.gov)
  • The pckA gene, encoding the gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK), was cloned by PCR amplification from the purple nonsulfur bacterium Rhodopseudomonas palustris No. 7. (asm.org)
  • Molecular characterization of ZSB13 was performed by 16S rRNA gene amplification which confirmed it as Pseudomonas oleovorans. (bvsalud.org)
  • For example, when grown in the presence of both glucose and maltose, Lactococcus lactis will produce enzymes to metabolize glucose first, altering its gene expression to use maltose only after the supply of glucose has been exhausted. (wikipedia.org)
  • Taken together, our data demonstrated that starch instead of glucose can promote colistin production probably by affecting the expression of colistin biosynthesis-related genes, as well as reducing the repression of glucose to a secondary metabolic product. (hindawi.com)
  • In Rhizobium species, a PEPCK-deficient mutant fails to grow on minimal medium with succinate or other citric acid cycle intermediates but can grow on glucose and glycerol as a sole carbon source, suggesting that PEPCK functions here as a key gluconeogenic enzyme ( 30 ). (asm.org)
  • Using a published sequence of ferulate esterase gene (GenBank: AY837784.1) from Burkholderia multivorans (Rashamuse et al. (usda.gov)
  • The genes encoding organophosphorus hydrolase (mpd and opd) and pyrethroid-degrading esterase gene (pytY) were cloned using a PCR cloning strategy. (omicsonline.org)
  • Katarina Persson is active in the research environment Inflammatory Responses and Infection Susceptibility Centre (iRiSC) and her main research interest is in the field of bacteria-host interactions during urinary tract infection. (oru.se)
  • Our group established the first laboratory cultures of phototrophic consortia that represent the only laboratory model system currently available for the study of highly specific interactions between nonrelated bacteria. (dsmz.de)
  • Gene regulation involves a complex web of interactions within a given cell among signals from the cell's environment, signalling molecules within the cell, and the cell's DNA. (pressbooks.pub)
  • These interactions lead to the expression of some genes and the suppression of others, depending on circumstances. (pressbooks.pub)
  • In this One-on-One, Medscape Editor-in-Chief Eric Topol talked with Dr Church about his many research interests, the promise and controversy of CRISPR gene editing, and how he never gets bored. (medscape.com)
  • SAR202, found in the deeper part of the ocean, specializes in hard-to-access carbon compounds that other bacteria can't access. (asm.org)
  • The growth curve reflected a diauxic growth other uses for it, in particular lignosulfonates originating from when mixtures of the model compounds were used as carbon the sulfite pulping process, but the predominant technical lig- source. (lu.se)
  • We want to investigate these unknown genes with respect to their influence on virulence, growth and essentiality. (uni-goettingen.de)
  • Here, a full-scale advanced wastewater treatment demonstration facility (ozone, biological activated carbon filtration, micro/ultrafiltration, reverse osmosis, and advanced oxidation) was sampled over three months. (stanford.edu)
  • Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)
  • Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
  • She has shown that a protein called DegU helps the individual bacteria to "decide" whether to form a biofilm or not. (sciencedaily.com)
  • DegU protein enables the bacteria to switch between swarming movement and the production of biofilm materials to suit the particular circumstances," said Dr Stanley-Wall. (sciencedaily.com)
  • 2010) Cellulosilyticum ruminicola, a newly described rumen bacterium that possesses redundant fibrolytic-protein-encoding genes and degrades lignocellulose with multiple carbohydrate- borne fibrolytic enzymes. (concordia.ca)
  • Silicon atoms outnumber carbon atoms in the Earth's crust more than 1,000-fold, yet the two elements are remarkably alike, chemically speaking. (businessinsider.com)
  • Lactate has also been described to generate the acetyl-CoA to provide the two carbon atoms for the acetate to n -butyrate elongation via reverse β-oxidation, in which the oxidation of lactate to pyruvate produces NADH and the conversion of pyruvate to acetyl-CoA with ATP generation. (biomedcentral.com)
  • A valeric acid substituent is attached to one of the 2 carbon atoms of the tetrahydrothiophene ring. (medscape.com)
  • Using a plate containing ethyl ferulate as sole carbon source, various bacteria cultures were screened for ferulate esterase (FAE). (usda.gov)
  • A R. palustris No. 7 PEPCK-deficient strain showed growth characteristics identical to those of the wild-type strain either anaerobically in the light or aerobically in the dark when a C 4 -dicarboxylic acid, such as succinate or malate, was used as a carbon source. (asm.org)
  • Butyrate and sucrose were the most significant EA and carbon source respectively for CA production from lactate by strain CPB6. (biomedcentral.com)
  • This Hydrogenophaga strain also expressed genes indicative of chemolithoautotrophy, including CO 2 fixation, H 2 oxidation, S-compound oxidation, and denitrification. (frontiersin.org)
  • These two genes play opposite roles in regulation of colistin production. (hindawi.com)
  • The cost of metabolic gene regulation outweighs the benefits in this particular case. (asm.org)
  • Thus, although most regulation of gene expression occurs through transcriptional control in prokaryotes, regulation of gene expression in eukaryotes occurs at the transcriptional level and post-transcriptionally (after the primary transcript has been made). (pressbooks.pub)
  • In this way, regulation of the transcription of all of the structural genes encoding the enzymes that catalyze the many steps in a single biochemical pathway can be controlled simultaneously, because they will either all be needed at the same time, or none will be needed. (pressbooks.pub)
  • Recent studies support a role for biotin in cell proliferation, DNA repair, epigenetic gene regulation as well as normal immune function. (medscape.com)
  • In addition, use of starch stimulated the expression of regulatory gene spo0A but repressed the expression of another regulatory gene abrB . (hindawi.com)
  • RIViT-seq technology could speed up associating transcription factors with their target genes. (doe.gov)
  • however, gene expression in eukaryotes is more complicated because of the temporal and spatial separation between the processes of transcription and translation. (pressbooks.pub)
  • This exciting finding suggests post-fire microbes can 'eat' methane to gain carbon and energy, and can potentially help us reduce greenhouses gases," said Pulido-Chavez. (earth.com)
  • Microbes in this area mainly use carbon sources generated by the surface-dwelling microbes. (asm.org)
  • These cells convert between 6-37% of the carbon fixed in the oceans daily. (asm.org)
  • PEPCKs have been generally classified according to their nucleotide specificities: enzymes from bacteria, yeast, and plants mainly use adenosine nucleotides, but enzymes from a variety of eukaryotes and mammals use guanosine or inosine phosphates ( 24 ). (asm.org)
  • Unlike the situation in bacteria, it was not known which genes were responsible for the activation of the urease enzyme in soybean. (usda.gov)
  • In bacteria and Arabidopsis thaliana, UreD and UreF, in addition to UreG, are essential for in vivo urease activation. (usda.gov)
  • Streptococcus pneumoniae strains lacking the enzyme dihydrolipoamide dehydrogenase (DLDH) show markedly reduced ability to grow on raffinose and stachyose as sole carbon sources. (lu.se)
  • Therefore, we collaborate within a consortium of research institutes in the MiniCell project to delete non-essential genes to create a much more reduced organism. (uni-goettingen.de)
  • This can also occur when the bacterium in a closed batch culture consumes most of its nutrients and is entering the stationary phase when new nutrients are suddenly added to the growth media. (wikipedia.org)
  • Life, of course, is everywhere, as we have explained, emerging on diverse worlds , carbon or silicon based or emerging on worlds humans would consider too poisonous for life. (zetatalk.com)