Gene and cell stem cell clone cells. Medical search. Frequent questions

In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
A year ago, we showed that you could do it with cells from embryos," says Wilmut. (newscientist.com)
Particularly valuable animals could be cloned from adult cells without the uncertainties of crossing them with other animals or tinkering with embryos. (newscientist.com)
More importantly, biotechnologists will for the first time be able to manipulate the genes of cells from farm animals directly before growing them into embryos. (newscientist.com)
But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)
Cloned human embryos express the genes required for pluripotency, but animal-human hybrids do not, according to a study published today (Feb. 2nd) in the journal__ linkurl:Cloning and Stem Cells. (the-scientist.com)
http://www.liebertonline.com/clo __The findings pave the way for isolating human embryonic stem cells from therapeutic cloning -- a landmark that has never been achieved after linkurl:Woo-suk Hwang's discredited cloning experiments;http://www.the-scientist.com/news/display/22933/ -- but call into question the utility of interspecies embryos. (the-scientist.com)
These eggs simply do not reprogram," lead author linkurl:Robert Lanza,;http://www.robertlanza.com/ chief scientific officer of Advanced Cell Technology in Worcester, Mass., said of the human-animal hybrid embryos. (the-scientist.com)
The MIT Technology Review published on Wednesday a news report about the first-known experiment to create genetically modified human embryos in the United States using a gene-editing tool called CRISPR. (cnn.com)
The MIT Technology Review reported that the researchers in Portland, Oregon, edited the DNA of a large number of one-cell embryos, specifically targeting genes associated with inherited diseases in those embryos. (cnn.com)
Previously, scientists in China were the first in the world to reveal attempts to modify genes in human embryos using CRISPR. (cnn.com)
Three separate papers were published in scientific journals describing various studies in China on gene editing in human embryos. (cnn.com)
The recent desperation to clone human embryos may be seriously undermining accepted ethical principles of medical research, with potentially profound wider consequences. (lifeissues.net)
Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)
Researchers reported in Nature on November 22, 2007, that they successfully isolated 2 embryonic stem cell lines from cloned embryos made using cells from the skin of an adult rhesus macaque. (nih.gov)
But cloning for therapeutic reasons - meaning, carefully regulated research into disease using human embryonic embryos - is an entirely different matter. (wtnnews.com)
Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)

One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
The Cell Culture Facility (CCF) located in the Life Science Laboratories supports research by providing space, equipment, cell cultures (plant and mammalian), media, supplies and training on a recharge basis for both academic labs and corporate partners. (umass.edu)
His research blossomed after he came to Roslin Institute where in a series of papers he put the intellectual framework into the method of mammalian cloning that ultimately led to the birth of Dolly in 1996. (nottingham.ac.uk)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)

Professor Campbell was a cell biologist/embryologist with a research career spanning more than 30 years, the majority of which was in the field of cell growth and differentiation. (nottingham.ac.uk)
His pioneering studies into cell-cycle control and cellular differentiation led to the programme of work at Roslin that gave birth to the first mammal to be cloned from adult cells - ie. (nottingham.ac.uk)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
Is the Subject Area "Cell differentiation" applicable to this article? (plos.org)
miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells. (genscript.com)
Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

The CRISPR/Cas9-mediated homologous recombination system with donor DNA oligonucleotides of the human sites of the corresponding gene sites was used to humanize the fourth and fifth exons. (eco-vector.com)
As a result of the study, a strategy for CRISPR/Cas9-assisted homologous recombination in the genome of mouse embryonic stem cells has been developed to create a fully humanized Snca gene encoding α-synuclein, and the clone genome of mouse embryonic stem cells has been edited using a CRISPR technology. (eco-vector.com)
In this One-on-One, Medscape Editor-in-Chief Eric Topol talked with Dr Church about his many research interests, the promise and controversy of CRISPR gene editing, and how he never gets bored. (medscape.com)

Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)

Topics covered will include cell membrane structure and function, metabolism, cell motility and division, genome structure and replication, the regulation of gene expression and protein production, genotype to phenotype relationship, and basic principles of inheritance. (middlebury.edu)
The expression of CCR5 is selectively induced during the cancer transformation process and is not expressed in normal breast or prostate epithelial cells. (wikipedia.org)
These regulatory mechanisms include: genetic deletions that abrogate cell surface expression of CCR5, downregulation of CCR5 on the surface of CD4+ T cells, in particular on memory cells, and delayed onset of CCR5 expression on the CD4+ T cells during development. (wikipedia.org)
Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. (nature.com)
Complex patterns of posttranslational modifications of chromatin including acetylation of histones establish combinatorial signals that are apparently interpreted by chromatin-binding proteins and the gene expression machinery to control transcriptional activity of individual genes. (aacrjournals.org)
Specificity Comments: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. (thermofisher.com)
An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. (thermofisher.com)
The non-agouti mutation in C57BL/6 strains is due to an 11.8 Kbp retrotransposon located in the first intron of the agouti gene that abolishes the expression of the Agouti gene. (umassmed.edu)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
Furthermore, ectopically expressed NPAS3 in PC12 cells produced parallel responses for nuclear factor kappa-light-chain-enhancer of activated B cells [NF-κB (P65)] expression, which specifies that NPAS3 regulates VGF through the NF-κB signaling pathway. (frontiersin.org)
Over-expression of NPAS3 also enhances the cell proliferation, which can be blocked by knockdown of VGF. (frontiersin.org)
The stemness of the cells were characterized by the sphere-forming capacity and the expression levels of RSCs markers. (bvsalud.org)
Here, we report that the stem/progenitor cell transcription factor, Sex determining region Y-box 2 (Sox2), is required in the embryonic SCN to control the expression of SCN-enriched neuropeptides and transcription factors. (bvsalud.org)
The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)
states'' or gene expression patterns. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Certain populations have inherited the Delta 32 mutation, resulting in the genetic deletion of a portion of the CCR5 gene. (wikipedia.org)
Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)
The copied material, which has the same genetic makeup as the original, is referred to as a clone. (medlineplus.gov)
Current Research and Scholarly Interests Genetic testing, gene therapy, genetically engineered organisms, and the history of eugenics. (stanford.edu)
Next, the researchers take cells containing donor genetic material. (newscientist.com)
There he continued his research on the cloning and genetic modification of livestock. (nottingham.ac.uk)
The resulting clones were plated on Petri dishes for propagation and a further genetic analysis. (eco-vector.com)
Clone 126-2F4 was found out carrying the necessary genetic modifications. (eco-vector.com)
Genetic manipulation of the mouse genome has been customarily performed using ES cells derived from the agouti 129-inbred strain of mouse. (umassmed.edu)
These ES cells allow for the generation of gene‐targeted mice on a C57BL/6 genetic background without the need for multiple backcrossing of mice. (umassmed.edu)
Banking of multiple cell lines with varying genetic spectrum that can be matched to patients is one possibility. (spiked-online.com)
Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
These techniques are based upon the preparation of hybrid genes, i. e. genes comprising genetic material encoding the polypeptide of interest linked to genetic material additional to the gene of interest. (justia.com)
Since then, the South Korean scientists have reported creating nearly a dozen new lines of human embryonic stem cells that for the first time carry the genetic signature of diseased or injured patients. (wtnnews.com)
A clone is an organism that is a genetic copy of an existing one. (who.int)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
Our work indicated that inhibition of Sox2 could change cell fate by affecting transcription factors in the development of bipolar cells and amacrine cells, may provide new directions for the study and treatment of retinal genetic diseases and retinal dysplasia. (bvsalud.org)
Genetic Diagnostic Technologies Genetic diagnostic technologies are scientific methods that are used to understand and evaluate an organism's genes. (msdmanuals.com)
In the now-famous "Dolly" experiments, cells from a sheep (donor cells) were fused with unfertilized sheep eggs from another sheep (recipient cells) from which the natural genetic material was removed by microsurgery. (msdmanuals.com)
Then the genetic material from the donor cells was transferred into the unfertilized eggs. (msdmanuals.com)
As expected, Dolly was an exact genetic copy of the original sheep from which the donor cells were taken, not of the sheep that provided the eggs. (msdmanuals.com)
Studies suggest that cloned higher animals (and thus humans) are more likely to have serious or fatal genetic defects than normally conceived offspring. (msdmanuals.com)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
Genetic analysis of dasatinib-treated chronic myeloid leukemia rapidly developing into acute myeloid leukemia with monosomy 7 in Philadelphia-negative cells. (lu.se)

C-C chemokine receptor type 5, also known as CCR5 or CD195, is a protein on the surface of white blood cells that is involved in the immune system as it acts as a receptor for chemokines. (wikipedia.org)
In humans, the CCR5 gene that encodes the CCR5 protein is located on the short (p) arm at position 21 on chromosome 3. (wikipedia.org)
The envelope glycoprotein structure consists of two protein subunits cleaved from a Gp160 protein precursor encoded for by the HIV-1 env gene: the Gp120 external subunit and the Gp41 transmembrane subunit. (wikipedia.org)
NPAS3 protein is also involved in other processes in the brain such as neurogenesis, circadian rhythm and cell proliferation. (frontiersin.org)
Genes and Chromosomes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or the code for functional ribonucleic. (msdmanuals.com)
Identified gene candidates will be studied further in the context of determining sensitivity of protein degrader drugs which are currently emerging as alternative therapies for leukemia. (lu.se)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
We detected all 3 genomic targets: the nucleoprotein, hemagglutinin, and large protein genes. (cdc.gov)

2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)

The most famous clone was a Scottish sheep named Dolly. (medlineplus.gov)
This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
The difference with Dolly is that all her DNA originated in a cell from the udder of an adult sheep. (newscientist.com)
Dolly was the only lamb born from 277 fusions of oocytes with udder cells. (newscientist.com)
Professor Campbell was instrumental in the creation of Dolly the Sheep, the first cloned mammal, a breakthrough which paved the way for the successful cloning of many other mammal species. (nottingham.ac.uk)
Dolly the Sheep, the first mammal to be cloned from an adult derived somatic cell, was born in 1996. (nottingham.ac.uk)
Inevitably most people will remember him for Dolly the sheep although his recent work was focused on fundamental and applied stem cell research as a tool for the study of human disease. (nottingham.ac.uk)
Researchers have been hoping to harness the therapeutic potential of cloning ever since the cloning of Dolly the sheep in 1997. (nih.gov)
When an embryo like this is implanted into a uterus, as with Dolly, the process is called reproductive cloning. (nih.gov)
Those were side effects during the process that led to the cloning of Dolly the sheep. (wtnnews.com)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)

Mouse embryonic stem cells are known to be tumorigenic - that is, they can grow uncontrollably and become cancerous. (spiked-online.com)
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. (genscript.com)

Possibility to work with primary human hematopoietic stem cells. (lu.se)

Furthermore, we found a distinctive type of retinal cells expressing the characteristic proteins of both bipolar cells and amacrine cells at P6, which may be an intermediate state in which amacrine cells were transforming into bipolar cells. (bvsalud.org)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Clinical manifestations of PNH occur when a HSC clone carrying somatic PIGA mutations acquires a growth advantage and differentiates, generating mature blood cells that are deficient of GPI-anchored proteins. (medscape.com)

WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)
General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)

In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)

Many tumor cell lines and experimental tumors respond to HDAC inhibition. (aacrjournals.org)
To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)
Spearman analysis was employed for correlation analysis of genes in tumor tissues of RB patients. (bvsalud.org)

The book is separated into three chapters covering biotechnology, animal cloning and human cloning. (progress.org.uk)
Whilst it is targeted at 14-18 year olds, 'Biotechnology and Cloning' assumes a high level of knowledge on an ambitious range of hard to grasp topics, none of which are directly explained in the book. (progress.org.uk)
Buy Biotechnology and Cloning from Amazon UK . (progress.org.uk)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

The following Vps35l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
Mus musculus RIKEN cDNA 9030624J02 gene (9030624J02Rik), transcript variant X1, mRNA. (genscript.com)

In addition to making sure researchers end up with the plasmid they want and generating large combinatorial libraries, j5 addresses "all the considerations of traditional multiple cloning site technology. (genomeweb.com)
The researchers wanted to see whether "mature" cells that have differentiated to fulfil a specialised role (such as that of an udder cell or a fetal cell) could be returned to a primitive state from which they could grow into entire organisms. (newscientist.com)
For each clone, the Roslin researchers combine material from two sources. (newscientist.com)
Researchers can insert new genes into these or knock out existing genes with great precision. (newscientist.com)
In the face of a shortfall of human egg donors, many researchers hope that by injecting human nuclei into animal eggs they will be able to obtain patient-specific human embryonic stem cells. (the-scientist.com)
Recently, researchers from the Sanger Institute (UK) have isolated stable and germline competent embryonic stem (ES) cells from C57BL/6N mice (1). (umassmed.edu)
Before this new study was published, Nature asked another group of researchers to confirm that the stem cells were genetically identical to the donor skin cells. (nih.gov)
The stem cells, the researchers showed, could turn into heart or nerve cells in the laboratory, and had other characteristics of established embryonic stem cell lines. (nih.gov)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)

This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)

MDS is a clonal disorder of myeloid stem cells. (medscape.com)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
Despite the recent success of tyrosine kinase inhibitors (TKIs) in the treatment of chronic myeloid leukemia (CML), approximately 2-17% of patients develop clonal cytogenetic changes in the Philadelphia-negative (Ph(-)) cell population. (lu.se)
Consequently, there is a need to characterize the clinical features of such cases and to increase our understanding of the pathogenetic mechanisms underlying the emergence of clonal cytogenetic changes in Ph(-) cells. (lu.se)
Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal hematopoietic stem cell (HSC) disease. (medscape.com)

And to investigate the potential role of p66Shc, we generated streptozotocin (STZ)-induced diabetic mice carrying p66Shc knockout (−/−) gene. (nature.com)
The aim of the study was to create a genetically modified clone of mouse stem cells with a conditional knockout of humanized α-synuclein, which can be used for the reinjection into mouse blastocysts, as well as for basic and applied in vitro research in the field of pathophysiology and neuropharmacology. (eco-vector.com)
To create mouse stem cells with a conditional knockout of the humanized Snca gene, a previously obtained clone with the first Snca exon flanked by LoxP sites, was used. (eco-vector.com)
In addition, these ES cells are the foundation for two large-scale knockout mouse programs designed to provide targeted BL/6 ES cells to the scientific community (EUCOMM and KOMP). (umassmed.edu)

The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)

Recent studies suggest that CCR5 is expressed in a subset of cancer cells with characteristics of cancer stem cells, which are known to drive therapy resistance, and that CCR5 inhibitors enhanced the number of cells killed by current chemotherapy. (wikipedia.org)
Shoukhrat Mitalipov, director of the Oregon Health & Science University's Center for Embryonic Cell and Gene Therapy, reportedly led the new research. (cnn.com)
Gene Therapy Although gene therapy is defined as any treatment that changes gene function, it is often thought of as the insertion of normal genes into the cells of a person who lacks such normal genes because. (msdmanuals.com)
Here we describe the case of a patient with CML who developed monosomy 7 in Ph(-) cells during dasatinib therapy. (lu.se)

By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
Those were spindle removal, donor cell fusion, and cytoplast activation. (news-medical.net)
The mitochondrial DNA of the stem cells, however, matched the donor egg's mitochondrial DNA. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
The resulting cells were pluripotent and could be differentiated into insulin-producing beta cells to restore the function of the pancreas in the donor. (news-medical.net)
Wilmut and his colleagues fuse the empty oocyte with the donor cell by bringing them together and subjecting them to an electric current. (newscientist.com)
Wilmut says there were so many failures because it is difficult to ensure that the empty oocyst and the donor cell are at the same stage of the cell division cycle. (newscientist.com)
Cas9 nuclease, single guide RNA, and donor DNA were transfected into mouse cells. (eco-vector.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Allogeneic hematopoietic stem cell transplantation is curative but it requires a histocompatible donor and is associated with significant morbidity and mortality, so it is reserved for severe cases of PNH with aplastic anemia or transformation to leukemia. (medscape.com)

However, cloning need not only be used to create a whole organism. (msdmanuals.com)
Whether a cell used for a clone produces a specific type of tissue, a specific organ, or an entire organism depends on the potential of the cell-that is, how highly the cell has developed into a particular type of tissue. (msdmanuals.com)
For example, certain cells called stem cells have the potential to produce a wide variety of tissue types or even possibly an entire organism. (msdmanuals.com)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)

Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
Since embryonic stem cells have the ability to form virtually any cell type in the body, those taken from a cloned embryo could potentially be used to treat many diseases. (nih.gov)

He then moved to PPLTherapeutics, the company that was spun out from Roslin Institute, where that procedure and his expertise led to the birth of cloned and genetically modified sheep, pigs and cattle. (nottingham.ac.uk)
An approach to obtaining clones of mouse genetically modified stem cells expressing pathological humanized α-synuclein, has been proposed and implemented. (eco-vector.com)
The developed cell clone can serve to create a line of genetically modified mice that serve as a test system for pathophysiological and neuropharmacological studies associated with synucleinopathies. (eco-vector.com)

Current Research and Scholarly Interests Recent clinical studies, by us and others, have demonstrated that genetically engineered T cells can eradicate cancers resistant to all other therapies. (stanford.edu)
Stem cell research and cloning, and egg procurement. (stanford.edu)
and stem cell research, written about by the Irish Council for Bioethics and the Telegraph among others. (progress.org.uk)
In 2007, a research team led by Mitalipov announced they created t he first cloned monkey embryo and extracted stem cells from it. (cnn.com)
The MIT Technology Review could not determine which disease genes had been chosen for editing in the new research. (cnn.com)
Finally, and inexorably, a true professional scientist poses clearly challenging questions to his research colleagues, and to the scientific enterprise in general, about the dubious "scientific" justification for the current rush to clone human beings - for both "therapeutic" and for "reproductive" purposes. (lifeissues.net)
Ethically, since eventually all such "research" will be applied to people, he cautions against the abuse of women "egg" donors, and against the premature use of vulnerable sick human patients for testing supposedly "patient-specific" stem cells in supposed "therapies", pointing to the obvious violations of standard international research ethics guidelines such clinical trials would necessarily entail. (lifeissues.net)
And he also agrees that if we don't find global agreement on human cloning, "we can probably expect dire consequences for the future of biomedical research and its impact on society at large. (lifeissues.net)
But he is equally concerned about the unethical aspects inherent in the rush to perform " therapeutic " human cloning research, including the abuses to all vulnerable human patients who would be required to participate in clinical trials. (lifeissues.net)
As he has questioned the HFEA before, would not the use of vulnerable human patients in clinical trials be premature, dangerous, and unethical given the already acquired knowledge in the research community that such supposed "patient-specific" stem cells would most probably cause serious immune rejection reactions in these patients? (lifeissues.net)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
Alternatively, research using eggs may point the way to methods which mimic their properties using other human cells and chemical agents. (spiked-online.com)
Two separate research teams have figured out how to "reprogram" cells with just a handful of genes to give them the characteristics of embryonic stem cells. (nih.gov)
After Gov. Jim Doyle vetoed a bill that would have banned cloning of human embryonic stem cells for research purposes, the legislative director of Wisconsin's Right to Life movement made a remark that seemed straight out of a science fiction movie. (wtnnews.com)
More to the point, it's not happening in Wisconsin, where opponents of stem cell research and related work recently persuaded the Legislature to pass a bill that would make criminals out of potential Nobel laureates. (wtnnews.com)
Doyle and a significant number of legislators from both parties simply want to hold open the possibility of continuing all forms of stem cell research in Wisconsin, the state that pioneered the process less than 10 years ago. (wtnnews.com)
The debate over stem cell research is complicated enough without "sci-fi" claims that mislead policymakers and the public. (wtnnews.com)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)

Through simply changing cell culture conditions, a newly established cell line, Erythroid Line from Lund University (ELLU), is able to differentiate toward mature cells, including enucleated reticulocytes. (lu.se)

We have discovered novel insights into the biology of human T cell exhaustion and developed approaches to prevent and reverse this phenomenon. (stanford.edu)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
In particular, the efficiency of the process will have to be improved before the technique could be applied in the clinic using human cells. (nih.gov)
First or all, scientists have been cloning human cells or their components for years. (wtnnews.com)

Genome-wide 500K SNP array analysis of the monosomy 7 clone revealed no acquired submicroscopic copy number changes. (lu.se)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

Thymidine analog-retention assays revealed that Sox2 deficiency contributed to reduced survival of SCN neurons during the postnatal period of cell clearance, but did not affect progenitor cell proliferation or SCN specification. (bvsalud.org)
Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
In this study, VGF (non-acronymic) was found regulated by NPAS3 in neuronal stem cells. (frontiersin.org)

cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

It might be expected that the richest nation on Earth would encourage its top scientists to pursue this work with vigor rather than limiting funding opportunities, creating legal barriers and fencing off any newly developed cell lines. (spiked-online.com)
just a few stories about scientists around the world who claim - falsely, of course - to have cloned babies. (wtnnews.com)
Other than a tiny number of weird scientists, it's hard to find anyone who likes the idea of implanting a cloned embryo into a woman's womb, risking not only the health of the "mother" but almost certainly producing babies with birth defects. (wtnnews.com)
South Korean scientists were the first to report successfully doing so in early 2004, but they produced just one cell line from 200 tries. (wtnnews.com)
Not even the South Korean scientists claim they're close to transplanting cells into a human, however. (wtnnews.com)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)

Although low ROS levels are beneficial to cellular stress responses for the activation of several cellular signaling pathways, abnormally elevated ROS leads to damage to cells and organs, and eventually, to cell death, thereby ROS can be either beneficial or detrimental to health 11 . (nature.com)
not for stem cells but for organs and body parts," Right to Life's Sue Armacost was quoted as saying. (wtnnews.com)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. (thermofisher.com)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)

If the same could be achieved in humans, it would mean that each of us could have clones of ourselves made from our own tissue. (newscientist.com)
Mammary glands are rich in these cells, which are more adaptable than other tissue. (newscientist.com)
Safeguards, such as suicide genes to destroy implanted tissue, will have to be considered. (spiked-online.com)

Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia. (thermofisher.com)
The accumulated data indicate that pathogenic mutations in the Snca gene are associated with the development of neurodegenerative brain damage, indicating the relevance of studying the synuclein neurobiological role. (eco-vector.com)
PNH is caused by somatic mutations in PIGA (which encodes phosphatidylinositol N-acetylglucosaminyltransferase subunit A) in one or more HSC clones. (medscape.com)

MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)

We are identifying new targets for these therapeutics, exploring pathways of resistance to current cell therapies and creating next generation platforms to overcome therapeutic resistance. (stanford.edu)
Ex vivo manufactured red blood cells (RBC) generated from immortalized erythroid cell lines which can continuously grow are expected to become a significant alternative in future transfusion therapies. (lu.se)

Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
He explained that there are multiple methods for cloning DNA. (genomeweb.com)
As an example, Vector NTI identifies restriction sites in a plasmid, which works well for the more traditional methods of assembling DNA, such as PCR, but Hillson notes that newer tools like SLIC and the Gibson method don't use restriction enzymes or multiple cloning sites in their protocols. (genomeweb.com)
METHODS: The retinoblastoma stem-like cells (RSLCs) were isolated by single cell cloning in combination of examination of sphere-forming capacities. (bvsalud.org)
The present report gives an overview of the terms and methods used in cloning and summarizes the debates in the General Assembly. (who.int)

This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)

A clone is a group of genetically identical cells or organisms derived from a single cell or individual. (msdmanuals.com)
The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)

They then remove the original chromosomes from these to leave DNA-free cells. (newscientist.com)
See also Genes and Chromosomes. (msdmanuals.com)
Unlike unfertilized eggs, these laboratory-made eggs had a complete set of chromosomes and genes. (msdmanuals.com)

You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart. (genscript.com)

ELLU is heterogeneous and, unexpectedly, clones expressing adult hemoglobin rapidly differentiate and produce fragile cells. (lu.se)

Thus, the Sanger team decided to repair the non-agouti mutation in the JM8 cells. (umassmed.edu)
Given the strong association between monosomy 7 and mutation of genes involved in the RAS pathway in juvenile myelomonocytic leukemia, we also screened for pathogenetic variants in KRAS, NRAS, and PTPN11, but did not detect any changes. (lu.se)

Transcription factors associated with the development of bipolar cells and amacrine cells also support those changes. (bvsalud.org)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

The latest experiments have also produced three lambs from the cells of a sheep fetus aborted after 26 days, and four from a nine-day-old embryo. (newscientist.com)
The UMass Chan TAMC has routinely utilized a subclone (MK6) of 129 SVevBrd derived ES cells in gene-targeting experiments. (umassmed.edu)
To generate suitable C57BL/6 embryonic stem cells for gene targeting experiments, the Sanger team established a male cell line (JM8) from the N-substrain of C57BL/6 mice. (umassmed.edu)
Two further sub-lines were then established: JM8.F6 (feeder-dependent) and JM8.N4 (feeder-free), that were tested for their performance in high-throughput gene targeting experiments. (umassmed.edu)
In these experiments the germline transmission rate exceeded 65% for multiple tested clones. (umassmed.edu)
One of the 'restored' clones (JM8A3) showed particularly favorable results in blast injection experiments. (umassmed.edu)

Cell 54 , 83-93 (1988). (nature.com)
Cell 54 , 95-104 (1988). (nature.com)
1988) Gene 67: 21-30), glutathione-S-transferase (Johnson (1989) Nature 338: 585-587), ubiquitin (Miller et al. (justia.com)

The process known as "DNA cloning," "molecular cloning" or "gene cloning" has been used widely since the 1970s. (wtnnews.com)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

Animal eggs no good for cloning? (the-scientist.com)

No immunoreactivity is seen with murine EC, EG or ES cells. (sigmaaldrich.com)

Previously, Mitalipov and his colleagues reported the first success in cloning human stem cells in 2013, successfully reprogramming human skin cells back to their embryonic state. (cnn.com)

Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
There are major obstacles to overcome before a stem cell line is liable to reach clinical trials. (spiked-online.com)
The dramatic increase in efficiency - more than one cell line for every 20 attempts - could pave the way for treating conditions such as diabetes and spinal cord injury with stem cell transplants. (wtnnews.com)
Still, the prospect of being able to study the root causes of a disease in an immortal, cloned line of stem cells is exciting enough. (wtnnews.com)

The following product was used in this experiment: SOX2 (Embryonic Stem Cell Marker) Recombinant Rabbit Monoclonal Antibody (SOX2/4267R) from Thermo Fisher Scientific, catalog # 6657-RBM7-P1. (thermofisher.com)

Anatomy61

Organisms14

Diseases9

Chemicals and Drugs66

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Phenomena and Processes57

Disciplines and Occupations4

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19972

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