• GenomePlex WGA is based on random fragmentation of genomic DNA and amplification by PCR using ligated adapters to create the OmniPlex™ Library. (sigmaaldrich.com)
  • Southern blots of genomic DNA's from A431 and other human cell lines were probed with either a v-erb B gene fragment or a human EGF receptor complementary DNA clone (pE7), previously isolated from an A431 complementary DNA library. (sciencemag.org)
  • History Gene amplification is a frequent manifestation of genomic instability that plays a role in tumour progression and development of drug resistance. (antibodyassay.com)
  • Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast tranferase gene, ALG7. (elsevier.com)
  • Thus, gene regions and intergenic "junk DNA" share a consistent picture for the genomic diversity of Pisum, despite low linkage disequilibrium in wild and landrace germplasm, which might be expected to allow independent evolution of these very different DNA classes. (genetics.org)
  • Furthermore, the genomic compartment in which the markers reside might affect the diversity pattern seen, and it is possible that markers residing mainly in junk DNA might produce different results from markers based upon genes, which are predominantly euchromatic. (genetics.org)
  • Quantification of the DNA was determined using the PicoGreen™ assay (Molecular Probes, Eugene, OR). (sigmaaldrich.com)
  • In the August 27, 2015, Federal Register , FDA announced it has classified the Clostridium difficile (C. difficile) toxin gene amplification assay into Class II (special controls) medical device. (mondaq.com)
  • Anin vitrotranscribed RNA standard of 970 nucleotides of the hemagglutinin gene was developed and used to determine the assay sensitivity. (stir.ac.uk)
  • Gene copy number analysis of FGFR1 gene was investigated by FISH assay (Cytovision, Berlin, Germany) according to the manufactures instructions. (biomedcentral.com)
  • A 14-gene multiplex assay conducted with RNA isolated from 20 FFPE blocks was correlated to an analysis of the same with reverse-transcription quantitative real-time PCR (RT-qPCR). (aaccjnls.org)
  • Detecting gene copy number changes is not straightforward because the assay must be quantitative ( 17 ). (aaccjnls.org)
  • In order to develop a species-specific PCR for the detection of Mycoplasma genitalium , the sequence of 1,490 bases of the 16S rRNA gene was determined for M. genitalium G37 (type strain) and four Danish isolates of M. genitalium . (asm.org)
  • Phylogenetic dendrogram showing the relationships between cloned 16S rRNA gene fragments from the Ellinbank pasture soil (env. (asm.org)
  • The 16S rRNA gene sequence from E. coli was used to root the dendrogram. (asm.org)
  • Phylogenetic analysis of 16S rRNA gene revealed that this microorganism is closely related to Bacillus gelatini . (springer.com)
  • 454 pyrosequencing analysis of the 18S rRNA gene revealed a highly diverse (35 phyla) and spatially variable eukaryotic community during water treatment and distribution. (rsc.org)
  • Activation of oncogenes and inactivation of tumour-suppressor genes have been shown to be influential on development and growth of human astrocytomas. (scirp.org)
  • Thus, selection favors a succession of gene-amplification types that make sequence changes more probable by adding targets. (nih.gov)
  • During the growth of a population under selection, these mutations are most likely to be gene amplifications. (pnas.org)
  • Mutations and gene amplifications in Endometrial Carcinomas. (uib.no)
  • Direct cycle DNA sequencing of ~450 bp of the PCR products of four C. trachomatis isolates revealed complete identity of one isolate with the known sequence of serovar F, while the other three isolates harboured three phenotypically silent point mutations at codons 96, 305 and 312 of the EF-Tu gene. (nus.edu.sg)
  • http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-9120&volume=&spage=&epage=&date=2005&atitle=Detecting+exon+deletions+and+duplications+of+the+DMD+gene+using+Multiplex+Ligation-dependent+Probe+Amplification+(MLPA). (hku.hk)
  • Amplification of the selected genes were assessed using MLPA, FISH and CISH and results were compared. (koreascience.or.kr)
  • Therefore, MLPA can serve as an alternative to ISH for detection of gene amplification. (koreascience.or.kr)
  • We used multiplex ligation-dependent probe amplification (MLPA) to detect PLP1 gene alterations, especially gene duplications and deletions. (aaccjnls.org)
  • We performed MLPA on 102 samples from individuals with diverse PLP1 gene abnormalities and from controls, including 50 samples previously characterized for the PLP1 gene by quantitative PCR but which were anonymized for prior results and sex. (aaccjnls.org)
  • MLPA is a rapid and reliable method to determine PLP1 gene copies. (aaccjnls.org)
  • Samples with partial PLP1 gene dosage alterations require confirmation with a non-MLPA method. (aaccjnls.org)
  • Multiplex ligation-dependent probe amplification (MLPA) is a technique for detecting duplications and deletions and is based on the ligation of 2 adjacent annealing oligonucleotides followed by quantitative PCR amplification of the ligated products( 24 ). (aaccjnls.org)
  • Specifically, scientists observed gene duplication in yeast exposed to high concentrations copper. (upi.com)
  • Analyzing the creation of the eccDNA supports our finding that gene duplication can be actively driven and controlled in response to the environment, rather than random, in order to confer increased resilience to future environmental change. (upi.com)
  • Gene amplification, also known as gene duplication or chromosomal duplication, is a cellular process in which multiple copies of a gene are produced. (wisegeek.com)
  • Sometimes, the amplified trait dies with the single organism that experiences the gene duplication. (wisegeek.com)
  • However, the relationship between gene duplication and the emergence of new biochemical functions is complicated, and it has been difficult to quantify the likelihood of evolving novelty in any systematic manner. (pnas.org)
  • However, the relationship between duplication and the emergence of new biochemical functions is complicated because gene duplications are generally expected to be either selectively neutral ( 5 ) or deleterious ( 6 ). (pnas.org)
  • In addition, evidence is provided for the very recent duplication of particular classes of R2R3 Myb genes in the grasses. (genetics.org)
  • Burkholderia pseudomallei acquired ceftazidime resistance due to gene duplication and amplification. (tropmedres.ac)
  • PLP1 gene duplication is the most common cause of PMD and accounts for ∼50%-75% of cases( 1 )( 2 )( 3 )( 6 )( 7 )( 8 )( 9 )( 10 ). (aaccjnls.org)
  • Animal models support PLP1 gene duplication as a molecular basis for disease because neurologic symptoms and disease severity in transgenic mice correlate with PLP1 gene copy number and overexpression ( 13 )( 14 ). (aaccjnls.org)
  • Learn gene amplification and duplication MCQs , MCAT biology test for learning online courses and test prep to practice. (mcqslearn.com)
  • MCAT biology practice test MCQ: a mechanism through which new genetic material is generated during molecular evolution is called with choices gene duplication, gene amplification, gene cloning and all of above problem solving skills for summative and formative assessment, competitive exam prep, interview questions with answer key. (mcqslearn.com)
  • Free study guide is for online learning gene amplification and duplication quiz with MCQs to practice test questions with answers. (mcqslearn.com)
  • Practice MCAT multiple choice questions on gene amplification and duplication, objective type quizzes for online classes on learning gene expression in prokaryotes tutorial . (mcqslearn.com)
  • The repair of DSBs mainly occurs through two mechanisms: non-homologous end-joining (NHEJ) and homologous recombination (HR). We previously showed that defects in the repair of DSBs via NHEJ could increase the frequency of gene amplification. (elsevier.com)
  • Some of the alternative technologies existing in the market include OmniPlex, Strand Displacement Amplification (SDA), Ligase Chain Reaction (LCR), and Hybrid Capture technology. (bio-medicine.org)
  • Quantitative real-time PCR amplifications were performed on an iCycler™ thermocycler (Bio-Rad, Hercules, CA) with SYBR ® Green dye (Cambrex BioScience Rockland, Rockland, ME) detection. (sigmaaldrich.com)
  • By the use of a relatively new quantitative method using PCR followed by ligase detection reaction (PCR/LDR), we have been able to detect small gene copy number changes at high sensitivity and reproducibility ( 16 - 18 ). (aacrjournals.org)
  • Single cell multiplex PCR amplification of five dystrophin gene exons combined with gender determination. (biomedsearch.com)
  • A target sequence such as the human glucokinase gene may be amplified with a high specificity, a high speed, a high sensitivity and a low cost through a multiplex PCR to detect a maturity-onset of diabetes of the young (MODY) 2 associated gene. (google.com)
  • Multiplex PCR amplifying 340 and 245 bp sequence of 38 kDa gene and IS6110, respectively was standardized and analytical sensitivity was verified. (nih.gov)
  • Analytical sensitivity of multiplex PCR was found to be 100 fg for 38 kDa gene and 1 fg for IS6110. (nih.gov)
  • The typical translocation partner is the NUP214 gene. (biomedcentral.com)
  • Here we report a unique case of a 44-year old patient with T-ALL in which the blasts demonstrated a derivative chromosome 9 involving a 9;22 translocation and a dicentric Philadelphia chromosome 22 with a homogeneously staining region at the interface of the 9;22 translocation, leading to BCR-ABL1 gene amplification. (biomedcentral.com)
  • In this report, we present a unique case of T-ALL/LBL with amplification of BCR-ABL1 fusion gene in an isodicentric derivative Philadelphia chromosome 22 (idic der (22) t (9;22) (q34;q11.2) hsr (9;22)) with a homogeneously staining region at the interface of the 9;22 translocation. (biomedcentral.com)
  • The 124 clone failed to detect BCL2 expression in the majority of translocation (+), amplification (+), and activated B-cell DLBCL cases in which high levels of BCL2 protein are expected. (elsevier.com)
  • Centrosome amplification (CA) is supposed to be one of mechanisms leading to chrosomal instability. (linkos.cz)
  • A broad-range bacterial PCR targeting rRNA genes (rDNAs) was used to directly analyze 536 clinical samples obtained from 459 hospitalized patients during a 4-year study period. (asm.org)
  • The rapid detection of MYCN gene amplification, especially in small samples obtained by aspiration or biopsy, has stimulated considerable interest in improved clinical management of neuroblastoma patients. (aacrjournals.org)
  • FGFR1 gene copy number (GCN) and the ratio of FGFR1:CH8 was compared to clinical and pathological characteristics and related to survival. (aacrjournals.org)
  • This review provides a comprehensive and updated discussion of the literature in the subject and critically discusses the main approaches, the pitfalls and provides practical suggestions for successful unbiased amplification of the whole transcriptome in clinical samples. (biomedcentral.com)
  • While the expression of a single or a limited number of genes can be readily estimated using minimum amount of total or messenger RNA (mRNA) from experimental or clinic samples, gene profiling requires large amount of RNA which can only be generated from global RNA amplification when using often limited amount clinical material. (biomedcentral.com)
  • Considering revealed clinical and gene expression heterogeneity between CA negative and CA positive patients, there is a possibility to characterize them as notable event in multiple myeloma pathogenesis. (linkos.cz)
  • Furthermore, the limited sensitivity of most microarray platforms requires time-consuming and costly amplification reactions of the target RNA, which negatively affects clinical laboratory work flow. (aaccjnls.org)
  • In humans, the presence of 3 or more copies of PLP1 is associated with severe disease, although patients with 3 and 5 PLP1 gene copies do not show significant differences in clinical signs( 15 ). (aaccjnls.org)
  • However, the presence of multiple amplifications in several patients with advanced primary tumours suggests that the accumulation of genetic changes may correlate more closely with tumour size than with inherent biologic aggression. (nih.gov)
  • Conversely, overexpression of MDM2 does not always correlate to gene amplification ( 12 , 14 ). (aacrjournals.org)
  • Increased concentration of DHFR in the cell: Amplification of the DHFR gene causes an increase in the amount of DHFR present and has been shown to correlate with reduced response to methotrexate treatment. (cancerquest.org)
  • To ward off environmental stress, living organisms accumulate extra copies of beneficial genes. (upi.com)
  • Duplicated copies of preexisting genes provide the primary genetic source for functional innovation ( 3 , 4 ). (pnas.org)
  • A cellular process characterized by the production of multiple copies of a particular gene or genes to amplify the phenotype that the gene confers on the cell. (thefreedictionary.com)
  • a process in which many copies are made of some genes at one time, while other genes are not replicated. (thefreedictionary.com)
  • MYCN amplification was identified by this differential PCR in all 10 samples carrying more than 10 copies (already known to have MYCN gene amplification by Southern blot analysis). (aacrjournals.org)
  • The creation of transgenic mice containing increased copies of the MDM2 gene and concomitantly displaying an increase in expression level had a 100% incidence of early tumor development ( 11 ). (aacrjournals.org)
  • By increasing the number of copies of a particular gene, the number of gene copies available for transcription could thereby be increased. (thefreedictionary.com)
  • In a number of instances of gene amplification, the amplification phenomenon appears to be developmentally regulated, and the amplified copies of the gene are subsequently lost from the cell. (thefreedictionary.com)
  • Assays for the frdA-linked ampC gene product, β-lactamase, indicated that the intact ampC gene is not incorporated in the λfrdA phages. (epfl.ch)
  • We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. (up.ac.za)
  • These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. (up.ac.za)
  • We have investigated whether a ERBB2 gene dosage effect might provoke increased biological aggressiveness and altered trastuzumab sensitivity. (ovid.com)
  • Gene amplification technology addresses applications in molecular biology research (DNA amplification), gene therapy, drug discovery, forensic identification, and diagnosis. (bio-medicine.org)
  • A popular approach for measuring the genetic diversity of accessions of a species is to analyze samples from gene banks (germplasm collections) by one or more of the many available molecular marker methods. (genetics.org)
  • however, the gene amplification status of molecular targeted genes in ESCC remains largely unclear. (spandidos-publications.com)
  • Six (55%) of the 11 gastric carcinoma cell lines and 15 (23%) of the 64 advanced gastric carcinomas showed the c-met gene amplification. (nih.gov)
  • Physiologically regulating proliferation, apoptosis and cell motility, gene amplification and subsequent over expression of these genes lead to relevant activation of pro-oncogenic pathways and correlates with response to tyrosine kinases inhibitors (TKI's). (biomedcentral.com)
  • 0.05) in the expression of these genes in different tissues, when compared among carotenoid-fed domesticated and wild fish, revealed the mechanism responsible for faded colouration and also revealed the means to enhance colour in the fish. (nature.com)
  • Using the method, we have constructed two chromosomal deletions in the chemotaxis gene region of S. typhimurium. (pnas.org)
  • These nonradioisotopic PCR-based protocols for detecting MYCN gene amplification and hTERT mRNA expression are rapid and reliable and are likely to be useful to determine the biological behavior of neuroblastoma. (aacrjournals.org)
  • CONCLUSION: These results support that KRAS amplification and KRAS mRNA expression, both increasing from primary to metastatic lesions, are relevant for endometrial carcinoma disease progression. (uib.no)
  • This model, termed adaptive radiation ( 10 ) or the innovation, amplification, and divergence (IAD) model ( 11 ), is rooted in the notion that many existing proteins display broad specificity and secondary activities in addition to the function that they evolved to carry out ( 12 ). (pnas.org)
  • While these studies did provide some clues on how vertebrate Myb genes evolved from a single ancestor, little information was obtained regarding the expansion of the plant R2R3 Myb gene family or about the evolution of the large number of plant Myb-domain proteins. (genetics.org)
  • UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. (wikipedia.org)
  • The UBB and UBC genes code for polyubiquitin precursor proteins. (wikipedia.org)
  • The different genes show large variation in diversity parameters, suggesting widely differing levels of selection and a high overall diversity level for the species. (genetics.org)
  • Ubiquitin is encoded in mammals by 4 different genes. (wikipedia.org)
  • This study confirms that the TransPlex Complete Whole Transcriptome Amplification Kit can effectively amplify low input quantities of RNA, approaching the single cell level. (sigmaaldrich.com)
  • Thermoinducible lysis-defective derivatives of λfrdA phages (λG1F and λG40F) carrying the fumarate marate reductase gene of Escherichia coli, inserted in each of two possible orientations, were used to amplify fumarate reductase synthesis and study the aerobic repression of frdA gene expression. (epfl.ch)
  • The detection of MYCN gene amplification is based on differential PCR in which three primer pairs were used to coamplify a 178-bp fragment of target MYCN gene with two reference gene fragments, a 237-bp of p53 exon 7 and a 120-bp of β-globin exon 3, in a single tube of 40 surgically resected tumor samples. (aacrjournals.org)
  • To detect MYCN gene amplification in neuroblastoma rapidly without using radioisotopes for diagnostic and therapeutic usefulness, we established a differential PCR protocol. (aacrjournals.org)
  • Among the 8 discordant samples, 6 cases were negative by IHC but positive by differential PCR and 2 cases with HER-2/neu amplification had normal HER-2/neu expression by IHC. (ac.ir)
  • Quantification of gene expression is a powerful tool for the global understanding of the biology underlying complex pathophysiological conditions. (biomedcentral.com)
  • The crossing-point (Cp) values for each amplification curve were calculated using the LightCycler ® 480 Relative Quantification software. (roche.com)