• Furthermore, alternatives and innovative & advanced technologies such as rolling circle amplification, loop mediated isothermal amplification, self-sustained sequence replication, and nucleic acid sequence based amplification, are expected to propel the market growth. (growthmarketreports.com)
  • Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to distinguish yaws from infections that cause similar skin lesions, primarily Haemophilus ducreyi . (cdc.gov)
  • In an attempt to develop a quick assay that can be deployed in the field for CLas detection, we developed a real-time loop-mediated isothermal amplification (rt-LAMP) assay by targeting the CLas five copy nrdB gene. (ppjonline.org)
  • Breast cancer with HER2 gene amplification or protein overexpression has special clinical features and biological behaviors, and the treatment regimen is very different from other types of breast cancer. (medxtmc.net)
  • It's common in various cancers, and it's linked to overexpression of the amplified gene (s). (amecoresearch.com)
  • ABCC3 (MRP3) protein overexpression was more common in tumors with gene amplification (P = 0.069). (lu.se)
  • MYC amplification and overexpression of its protein product has beenreported in 15-30% of gastric neoplasias. (waocp.org)
  • This phenomenon, known as gene amplification, results in overexpression of ALK receptor tyrosine kinase. (medlineplus.gov)
  • HER2 overexpression or HER2 gene amplification as determined by an accurate and validated assay (see sections 4.4 and 5.1). (who.int)
  • The transmembrane receptor-like protein encoded by the proto-oncogene Human Epidermal Growth Factor Receptor 2 (HER2) gene has tyrosine kinase activity, and HER2 is an important prognostic factor for breast cancer. (medxtmc.net)
  • Conclusions ESR1 amplification and activating MAP3K mutations are potential drivers of acquired resistance to adjuvant ETs employing estrogen deprivation in HR+ HER2- BC. (unimi.it)
  • The results of ABCC3 were correlated with the amplification status of HER2 and topoisomerase II alpha (TOP2A), which are located close to ABCC3 at 17q12-q21. (lu.se)
  • Of the cell lines studied 6 HER2-positive lines and 1 HER2-negative line exhibited amplification of ABCC3. (lu.se)
  • Specifically, increased ABCC3 mRNA and gene copy numbers were most prominent in HER2 amplified and/or HER2-enriched classified tumors. (lu.se)
  • A case of metastatic breast cancer with HER2 gene amplification that responded completely to single agent trastuzumab. (elsevierpure.com)
  • Herceptest (DakoCytomation, Glostrup, Denmark) showed 2 + staining and HER2 amplification was detected by fluorescent in situ hybridization. (elsevierpure.com)
  • Mutations in keratin genes have been documented in many disorders of the skin, nails, hair, and mucous membranes. (nih.gov)
  • Molecular surveillance is needed not only for the detection of mutations to the P . falciparum kelch gene, which are associated with artemisinin resistance [ 3 ], but also molecular markers associated with the efficacy of other antimalarial drugs. (researchsquare.com)
  • Mutations may be the cause of naturally occurring gene amplification. (amecoresearch.com)
  • Using a customized panel, we comprehensively evaluated gene mutations and copy number variation (CNV) in paired primary and metastatic specimens. (unimi.it)
  • Along with previously reported genomic alterations, including PIK3CA, TP53, CDH1, GATA3 and ESR1 mutations/deletions, we found that ESR1 gene amplification (confirmed by FISH) and MAP3K mutations were enriched in metastatic lesions as compared to matched primary tumors. (unimi.it)
  • The T-ARMS-PCR methodology allowed the identification of wild-type genotypes and mutated homozygous genotypes in polymorphisms G4 and G6 of GDF9 , whereas mutations in the BMP15 gene were not found. (ajvs.cl)
  • At least 16 mutations in the ALK gene have been identified in some people with neuroblastoma, a type of cancerous tumor composed of immature nerve cells (neuroblasts). (medlineplus.gov)
  • Neuroblastoma and other cancers occur when a buildup of genetic mutations in critical genes-those that control cell proliferation or differentiation-allows cells to grow and divide uncontrollably to form a tumor. (medlineplus.gov)
  • Less commonly, gene mutations that increase the risk of developing cancer can be inherited from a parent. (medlineplus.gov)
  • Somatic mutations in the ALK gene occur during the development of some cases of sporadic neuroblastoma, and inherited mutations in the ALK gene increase the risk of developing familial neuroblastoma. (medlineplus.gov)
  • Mutations in the ALK gene change single protein building blocks (amino acids) in ALK receptor tyrosine kinase. (medlineplus.gov)
  • Some mutations in the S gene may lead to changes in the spike protein which result in inhibition of contact and entry of the virus into human cells, however in the case of the VOC, they contain mutations in the S gene that enhance the process of contact and entry into human cells, increasing transmissibility of the virus. (who.int)
  • Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)
  • The global gene amplification technologies market size is estimated to register a significant CAGR during the forecast period, 2021-2028. (growthmarketreports.com)
  • The report on the global gene amplification technologies market includes an assessment of the market, trends, segments, and regional markets. (growthmarketreports.com)
  • From 2021 to 2028, the global Gene Amplification Technologies Market is predicted to grow at a CAGR of 8.6%, from US$ 26,489 Million in 2020 to US$ 50,636 Million by 2028. (amecoresearch.com)
  • The report provides annual sales estimates and projections for the global gene amplification technologies market for the years 2009 through 2017. (rapidmicromethods.com)
  • Nondiploid tumors had a significantly greater rate of HER-2/neu amplification compared with diploid tumors (P = .0003). (nih.gov)
  • The authors conclude that HER-2/neu gene amplification status can be determined by FISH on archival prostate cancer specimens, significantly correlates with high tumor grade and nondiploid DNA content, and is more frequently encountered in tumors with advanced pathological stage. (nih.gov)
  • In this study 22 genes were analyzed by MLPA in tumors and matched normal tissue samples from 82 patients with non-small cell lung cancer. (jcancer.org)
  • The MYC proto-oncogene, which is located at 8q24.1, regulates 15% of genes and isactivated in 20% of all human tumors. (waocp.org)
  • This cell line may represent a useful model to investigate the significance of the K-ras gene product for the pathogenesis of human tumors. (johnshopkins.edu)
  • This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
  • In order to reduce the cost of testing, a multiplex ddPCR assay of two target genes, pfmdr1 and pfplasmepsin2, was validated. (researchsquare.com)
  • In ER+ cancer cells, PIP5K1α acted on pSer-473 AKT, and was in complexes with VEGFR2, serving as co-factor of ER-alpha to regulate activities of target genes including cyclin D1 and CDK1. (lu.se)
  • The gold standard for the laboratory diagnosis of COVID-19 is the reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay, which searches for SARS-CoV-2 target genes in nasopharyngeal/oropharyngeal (NP/OP) samples, and its performance depends on the quantity and quality of the RNA input. (bvsalud.org)
  • Transcription-mediated amplification, an isothermal, single-tube nucleic acid amplification system utilizing two enzymes, RNA polymerase and reverse transcriptase, to rapidly amplify the target RNA/DNA, enabling the simultaneous detection of multiple pathogenic organisms in a single tube. (wikipedia.org)
  • Several whole genome amplification (WGA) protocols have been developed with reported varying representation of genomic regions post amplification. (diva-portal.org)
  • Overall this data indicate a limited random loss of genomic regions supporting the use of whole genome amplification for genomic analysis. (diva-portal.org)
  • Gene amplification is one of the most essential technologies utilized in genomic projects and also plays a key role in other areas including diagnostics. (growthmarketreports.com)
  • TE-rich genomic islands in expanded fungal ( P. fijiensis , L. maculans ) and oomycete ( Phytophthora infestans ) genomes carry genes that code for lineage-specific, putative small, secreted proteins. (biomedcentral.com)
  • Patients with ecDNA-containing tumours have significantly shorter survival, even compared to other forms of genomic focal amplification 3 . (nature.com)
  • Some of the alternative technologies existing in the market include Strand Displacement Amplification (SDA) and Ligase Chain Reaction (LCR). (rapidmicromethods.com)
  • However, the amount of DNA or the number of genes can also increase within an organism through gene duplication, a major mechanism through which new genetic material is generated during molecular evolution. (wikipedia.org)
  • Growing research & development in the molecular diagnostics field is another important aspect that propels the gene amplification technologies industry. (amecoresearch.com)
  • Gene Amplification Technology addresses applications, such as molecular biology research, gene therapy, drug discovery, forensic identification and diagnosis, and is one of the rapidly growing markets in the area of life sciences. (rapidmicromethods.com)
  • Molecular diagnostics and the Human Genome Project (HGP) have been the major growth drivers for gene amplification technology over the years. (rapidmicromethods.com)
  • Burgeoning demand for molecular diagnostics in clinical settings is expected to drive the miniaturization of DNA-amplification technologies. (rapidmicromethods.com)
  • In silico analysis of 804 breast cancers with matched gene expression and copy number microarray data revealed significant differences ABCC3 across the molecular subtypes. (lu.se)
  • the resistance pattern to -lactams of these strains was determined by using the disc diffusion and E-test methods followed by molecular methods such as PCR of bla et bla genes. (who.int)
  • Droplet digital PCR (ddPCR) assays have been developed for accurate assessment of CNVs in several human genes. (researchsquare.com)
  • The aim of the present study was to develop and validate ddPCR assays for detection of the CNVs of P. falciparum genes associated with resistance to antimalarial drugs. (researchsquare.com)
  • The gene copy number (GCN) quantification limit, as well as the accuracy and precision of the ddPCR assays were determined and compared to conventional quantitative PCR (qPCR). (researchsquare.com)
  • There were no significant differences between the GCN results obtained from uniplex andmultiplex ddPCR assays for detection of CNVs in the pfmdr1 and pfplasmepsin2 genes ( p = 0.363 and 0.330, respectively). (researchsquare.com)
  • There was no significant difference in gene copy numbers assessed by uniplex or duplex ddPCR assays regarding CNV in the pfgch1 gene ( p = 0.276). (researchsquare.com)
  • The developed ddPCR assays are simple, accurate, precise and cost-effective tools for detection of the CNVs in the pfmdr1 , pfplasmepsin2 and pfgch1 genes of P. falciparum . (researchsquare.com)
  • Present study was designed to explore the genetic diversity of plasmodium vivax genotypes based on Pvmsp-3α and Pvmsp - 3β genes using allelic specific nested PCR and RFLP assays markers from field isolates in district Mardan, Pakistan. (scielo.br)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
  • PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5? (nig.ac.jp)
  • alias" : "DRX002801", "center_name" : "UT_COB", "accession" : "DRX002801", "TITLE" : "PCR amplification of 16S rRNA gene (V1-V2 region) sequence", "STUDY_REF" : { "refname" : "DRP000910", "refcenter" : "UT_COB", "accession" : "DRP000910", "IDENTIFIERS" : { "PRIMARY_ID" : { "label" : "BioProject ID", "content" : "PRJDB2343" } } }, "DESIGN" : { "DESIGN_DESCRIPTION" : "", "SAMPLE_DESCRIPTOR" : { "refname" : "DRS002885", "refcenter" : "UT_COB", "accession" : "DRS002885", "IDENTIFIERS" : { "PRIMARY_ID" : { "label" : "BioSample ID", "content" : "SAMD00010272" } } }, "LIBRARY_DESCRIPTOR" : { "LIBRARY_STRATEGY" : "AMPLICON", "LIBRARY_SOURCE" : "METAGENOMIC", "LIBRARY_SELECTION" : "PCR", "LIBRARY_LAYOUT" : { }, "LIBRARY_CONSTRUCTION_PROTOCOL" : "PCR amplification of the SSU rRNA gene V1-V2 region using forward primer (5? (nig.ac.jp)
  • Validation of whole genome amplification for analysis of the p53 tumor suppressor gene in limited amounts of tumor samples. (diva-portal.org)
  • Chromosomal alterations are frequent events in lung carcinogenesis and usually display regions of focal amplification containing several overexpressed oncogenes. (jcancer.org)
  • The authors studied DNA amplification of the HER-2/neu gene on 4-microm sections obtained from 62 formalin-fixed, paraffin-embedded PCs by fluorescence in situ hybridization (FISH). (nih.gov)
  • The aim of this investigation was to find the relative efficacy of CISH(chromogenic in situ hybridization) or IHC (immunohistochemistry) in diagnosis and prognosis of gastric cancer,as well as the relationship of amplification and expression of C-MYC gene with patient survival. (waocp.org)
  • Whereas total RNA from whole tissue samples is often amplified one round before hybridization, a second round of amplification is generally performed on total RNA isolated from LDM samples to yield sufficient RNA for microarray approaches. (biomedcentral.com)
  • In each of the two cases, the amplified cyclin gene was overexpressed at the protein or mRNA level. (elsevierpure.com)
  • The amounts of K-ras specific mRNA and the Mr21,000 protein encoded by the amplified gene are correspondingly elevated. (johnshopkins.edu)
  • To facilitate future mutation detection analyses, we have therefore characterized the intron-exon organization of the human keratin 15 and keratin 19 genes. (nih.gov)
  • For example, a DNA methyltransferase gene in the wheat pathogen Zymoseptoria tritici (synonym Mycosphaerella graminicola ) was amplified to tens of copies, all of which were inactivated by Repeat-Induced Point mutation (RIP) including the original, resulting in loss of cytosine methylation. (biomedcentral.com)
  • Aneuploidy, TP53 mutation, and amplification of MYC correlate with increased intratumor heterogeneity and poor prognosis of breast cancer patients. (bvsalud.org)
  • We conclude that quantitative measurements of intratumor heterogeneity by multiplex FISH, detection of MYC amplification and TP53 mutation could augment prognostication in breast cancer patients . (bvsalud.org)
  • In this study, we used the multiple ligation probe amplification (MLPA) tool for the identification of female carriers of DMD deletions or duplications in 12 families with a single affected male, 10 of which were previously diagnosed as carriers of a DMD rearrangement, and the remaining two as having an unknown disease-causing mutation. (unich.it)
  • The objective of this study was to identify polymorphisms and their genotypes in the growth differentiation factor 9 ( GDF9 ) and bone morphogenetic protein 15 ( BMP15 ) genes in Pelibuey sheep using the tetra-primer amplification-refractory mutation system through polymerase chain reaction (T-ARMS-PCR). (ajvs.cl)
  • Arg1275Gln has been found in both familial and sporadic neuroblastoma and is the only common ALK gene mutation that has been found in both types of the condition. (medlineplus.gov)
  • The growth of the market is attributed to the rising demand for genetic information for the purpose of identifying & planning all the genes of the human genome. (growthmarketreports.com)
  • Gene amplification is a genetic aberration that occurs frequently in human cancer cells and involves many additional copies of a subchromosomal DNA region (amplicon). (amecoresearch.com)
  • The relative frequency of amplification in specific tumor subtypes, as well as its prevalence in 'early' preneoplastic lesions, point to a genetic origin. (amecoresearch.com)
  • The genetic status of cyclin genes was examined in a panel of 47 colorectal carcinoma cell lines. (elsevierpure.com)
  • Genetic testing is one such solution that makes use of gene amplification technology, which bodes the potential larger role that gene amplification technology could play in the area of diagnostics. (rapidmicromethods.com)
  • PCR allows amplification of genetic material in large quantities, and also enables the diagnosis of widespread diseases such as HIV. (rapidmicromethods.com)
  • Genetic diversity in nested PCR products was determined by Restriction Fragment Length Polymorphism (RFLP) utilizing Alu1 and PstI restriction enzymes for alpha and beta gene products digestion, respectively. (scielo.br)
  • For analysis the genetic diversity of the sub allelic variants of Pvmsp3α and Pvmsp3β genes, Chi-Square test was performed by utilizing Minitab programming software 18. (scielo.br)
  • Gene duplication is long recognized as a potential source of genetic innovation ( Ohno, 1970 ). (elifesciences.org)
  • Rearrangements of genetic material involving the ALK gene on chromosome 2 increase the risk of developing several other types of cancer. (medlineplus.gov)
  • Here we aim at investigating PD-L1 expression in erlotinib-resistant lung cancer cells with MET proto-oncogene ( MET ) gene amplification. (oncotarget.com)
  • It is one of the most effective tools for gene amplification, which allows researchers to achieve millions of copies of a gene in a short period of time. (growthmarketreports.com)
  • Gene amplification is a biological process in which copies of a gene or genes are produced to increase the phenotype that the gene confers on the cell. (amecoresearch.com)
  • Qualitative PCR is used for applications, such as monitoring gene expression levels and viral load. (rapidmicromethods.com)
  • The viral S gene is important as it codes for the Spike protein which is the molecule that makes contact with, and allows entry of the virus into susceptible host cells, causing infection. (who.int)
  • They were used for the ( RdRP ) viral genes 1-4 . (bvsalud.org)
  • Transposable elements (TEs) can be key drivers of evolution, but the mechanisms and scope of how they impact gene and genome function are largely unknown. (biomedcentral.com)
  • To further test the effects of transposon-aided gene amplifications on genome evolution and architecture, the repetitive fraction of the significantly expanded genome of the banana pathogen, Pseudocercospora fijiensis , was analyzed in greater detail. (biomedcentral.com)
  • These results indicate that the interplay of TEs and RIP can result in different and unpredictable fates of amplified genes, with variable effects on gene and genome evolution. (biomedcentral.com)
  • TEs have been detected in the genomes of both prokaryotic and eukaryotic organisms [ 1 ], and have been rightly labeled as 'drivers of genome evolution' [ 2 ] due to their direct and indirect impacts on genes and genomes. (biomedcentral.com)
  • In this study, we investigated the bias of a second round of amplification on microarray results by comparing two rounds of amplification with one round using the Agilent Whole Human Genome platform. (biomedcentral.com)
  • By performing FISH with a multiplexed panel of 10 probes to enumerate copy numbers in individual cells , and by sequencing 563 cancer -related genes , we analyzed how aneuploidy is linked to intratumor heterogeneity. (bvsalud.org)
  • Results showed that correct amplicon sequencing is the bovine growth hormone gene showed 99% homology with the GH gene Bos indicus Breed Butane that has been published in GenBank. (sunankalijaga.org)
  • Using long sequencing reads from the Oxford Nanopore Technologies platform, we phased SNVs within large gene copy arrays for the first time. (elifesciences.org)
  • Identification of deletions and duplications of the DMD gene in affected males and carrier females by multiple ligation probe amplification (MLPA). (unich.it)
  • These data suggest that a variety of cyclin genes can play a role in human tumorigenesis and that cyclins D2 and E are particularly important in a subset of colorectal neoplasms. (elsevierpure.com)
  • Baykara O, Bakir B, Buyru N, Kaynak K, Dalay N. Amplification of Chromosome 8 Genes in Lung Cancer. (jcancer.org)
  • The most frequently amplified genes were ZNF703, PRDM14 and MYC on chromosome 8 and the BIRC5 gene on chromosome 17. (jcancer.org)
  • Amplification of the ZNF703, PRDM14 and MYC genes were highly correlated suggesting that the genes displaying high copy number changes on chromosome 8 collaborate during lung carcinogenesis. (jcancer.org)
  • The NDR2 gene encodes a protein kinase important for neuronal development and is part of a microduplication region on chromosome 12 that is associated with intellectual disabilities, autism, and epilepsy. (uni-halle.de)
  • Gain or amplification of chromosome arm 1q21 (1q21+) is the most frequent adverse chromosomal aberration in MM, occurring in 40% of patients at diagnosis. (healthcare-biotech.com)
  • The S gene is one of the structural genes of the virus which encodes for a protein that sits on the surface of the SARS CoV-2 virus. (who.int)
  • The research study on the Gene Amplification Technologies Market also offers a regional analysis, wherein, demand across every region has been comprehensively analyzed. (timestechpharma.com)
  • DNA replication is a natural form of copying DNA with the amount of genes remaining constant. (wikipedia.org)
  • A piece of DNA or RNA that is the source and/or product of either natural or artificial amplification or replication events is called an amplicon. (wikipedia.org)
  • Uneven crossing-over between mismatched homologous chromosomes during meiosis, aneuploidy, polyploidy, retrotransposition and replication slippage are all possible causes of gene amplification. (amecoresearch.com)
  • Amplification could be aided by chromosomal fragile spots, DNA replication abnormalities, or telomere malfunction. (amecoresearch.com)
  • Currently, a wide range of amplification protocols has been developed to increase the amount of RNA for microarray approaches [ 2 ]. (biomedcentral.com)
  • These primers amplify approximately 495 bp of exons and introns at the 5' end of the β-tubulin gene. (cdc.gov)
  • A multiplex ddPCR assay was developed to detect the CNVs in the pfmdr1 and pfplasmepsin2 genes, while a duplex ddPCR assay was developed to detect CNV in the pfgch1 gene. (researchsquare.com)
  • In addition, the CNVs of genes of field samples collected from Thailand from 2015 to 2019 (n = 84) were assessed by ddPCR and results were compared to qPCR as the reference assay. (researchsquare.com)
  • Based on the obtained gene copy number quantification limit, the accuracy and percent relative standard deviation (%RSD) value of the multiplex ddPCR assay were 95% and 5%, respectively, for detection of the CNV of the pfmdr1 gene, and 91% and 5% for detection of the CNV of the pfplasmepsin2 gene. (researchsquare.com)
  • The results suggested that multiplex ddPCR assay is the optional assay for the accurate detection of gene copy number without requiring calibration standards, while the cost and required time are reduced. (researchsquare.com)
  • The least sensitive target gene and the gene most affected by RNA extraction procedures was the RNA-dependent RNA polymerase gene (Charité-Berlin protocol). (bvsalud.org)
  • Extrachromosomal double-minute chromosomes (dmins) or chromosomally integrated homogeneously stained areas are cytogenetically indicative of amplification (HSRs). (amecoresearch.com)
  • Primer sequences for the amplification of the NMNAT1 gene. (molvis.org)
  • Newly developed gene amplification tests can also detect noninfectious SV40 sequences. (who.int)
  • Although there is no evidence of SV40 sequences in OPV, the Expert Committee agreed to the introduction of a gene amplification test for SV40 in poliovirus seed stocks to provide an additional level of security. (who.int)
  • Previous analyses revealed that TE-mediated gene amplifications can have variable effects on fungal genomes, from inactivation of function to production of multiple active copies. (biomedcentral.com)
  • Detection of a methylcarbamate degradation gene in agricultural soils using PCR amplification of bacterial community DNA. (cdc.gov)
  • Soil bacterial community DNA was extracted and humic acid contaminants removed prior to PCR amplification of mcd. (cdc.gov)
  • Objective: To detect the presence and transmission of S. mutans carrier of the spaP gene in samples of bacterial plaque in mother/child pairs from municipal child education centers, and the possible association with dental caries. (bvsalud.org)
  • We therefore conclude that gene amplification can occur spontaneously in cells and that the rate of its occurrence varies with gene copy number. (wustl.edu)
  • Moreover, amplification of the P . falciparum GTP cyclohydrolase 1 ( pfgch1 ) GCN is linked to upregulation of the Plasmodium falciparum dihydrofolate reductase ( pfdhfr ) and P . falciparum dihydropteroate synthase ( pfdhps ) genes, which are associated with sulfadoxine-pyrimethamine resistance in Southeast Asia [ 9 , 10 ]. (researchsquare.com)
  • We have determined whether the gene encoding dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP + oxidoreductase, EC 1.5.1.3) varies spontaneously in gene copy number in cells in vitro. (wustl.edu)
  • After 10 successive rounds of growth and sorting, the derived population showed a 50-fold increase in fluorescence intensity, was highly resistant to methotrexate, and was amplified 40-fold in content of dihydrofolate reductase gene. (wustl.edu)
  • Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are caused in the majority of cases by deletions of the DMD gene and are readily detectable in affected males by multiplex polymerase chain reaction (PCR). (unich.it)
  • The frequency of deletions were much lower and the most frequently deleted gene was ADAM9. (jcancer.org)
  • These primers amplify approximately 717 bp of the coding region of the EF-1α gene. (cdc.gov)
  • There is a special set of ITS primers specifically for amplification of the ITS region of dermatophytes, especially Trichophyton (Gräser, 2000). (cdc.gov)
  • By extraction an accurate DNA and using suitable primers, H6H gene can be isolated completely. (ac.ir)
  • We also found that cells already having amplified genes can undergo increases or decreases in their fluorescence and in gene copy number even more rapidly (at rates as high as 3 x 10 -2 amplification events per cell division) than do parental cells (ca. 10 -3 events per division). (wustl.edu)
  • Therefore, we strongly recommend improvement of amplification procedures and importance of microarray probe design to allow detection of all differentially expressed genes in case of limited amounts of RNA. (biomedcentral.com)
  • Other primer sets have been used for other genes, but those described below are the most consistently available in databases for the identification of yeasts and molds that are most likely to be identified in a clinical microbiology laboratory. (cdc.gov)
  • Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. (cdc.gov)
  • The process of amplification of bovine growth hormone gene carried by one pair of primer. (sunankalijaga.org)
  • There is ongoing discussion on the effect of a second round of amplification on the reliability of microarray data. (biomedcentral.com)
  • This test helps deciding treatment for Carcinoma breast patients , who show positive reaction with Her2neu antibody and or gene amplification with FISH cytogenetic technique.Transtuzumab is the drug used to treat patients who are Positive for Her2neu. (blallab.com)
  • We focused on the gene amplification and protein expression of ABCC3 (also known as MRP3) in breast cancer cell lines and clinical tumor samples. (lu.se)
  • Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. (uchicago.edu)
  • The ALK gene provides instructions for making a protein called ALK receptor tyrosine kinase, which is part of a family of proteins called receptor tyrosine kinases (RTKs). (medlineplus.gov)
  • Targeted inhibition of MET significantly decreases both gene and protein expression of PD-L1. (oncotarget.com)
  • The research report on the Gene Amplification Technologies Market offers an extensive analysis of how the postoperative pain therapeutics landscape would evolve through 2026. (timestechpharma.com)
  • Reverse transcriptase PCR (RT-PCR) is a powerful tool for gene expression analysis because of its sensitivity, speed, ease of use, and versatility. (rapidmicromethods.com)
  • Our analysis uncovered a mechanism of adaptive SNV homogenization reminiscent of gene conversion, which is actively driven by selection. (elifesciences.org)
  • The increasing prevalence of cancer in the world is the primary factor boosting the gene amplification technologies market. (amecoresearch.com)
  • Acumen Research and Consulting has recently published a research report on the Gene Amplification Technologies Market for the forecast period of 2022-2030, wherein, the global market has been analyzed and assessed in an extremely comprehensive manner. (timestechpharma.com)
  • The research study on the Gene Amplification Technologies Market analyzes key drivers upholding product sales and prominent trends shaping the growth of the market. (timestechpharma.com)
  • Global market for gene amplification technologies is led by the US, which is also the fastest growing market worldwide, as stated by the new market research report on Gene Amplification Technologies. (rapidmicromethods.com)
  • However, growth in the gene amplification technologies market would be driven by other gene amplification technologies, which are forecast to display stronger growth worldwide. (rapidmicromethods.com)
  • The research report titled "Gene Amplification Technologies: A Global Strategic Business Report" announced by Global Industry Analysts Inc., provides a comprehensive review of the gene amplification technologies market, current market trends, key growth drivers, new product innovations/launches, recent industry activity, and profiles of major/niche global market participants. (rapidmicromethods.com)
  • It is known that the amplification procedure can introduce a bias by truncating the length of the amplified transcripts due to inefficient cDNA synthesis. (biomedcentral.com)
  • We have recently reported that ascitic cells from a human ovarian carcinoma have a 10- to 20-fold K-ras amplification and that the level of such amplification did not change over a 9-month period during which the patient received chemotherapy and underwent clinical progression. (johnshopkins.edu)
  • Insulin-like growth factor and epidermal growth factor independence in human mammary carcinoma cells with c-erbB-2 gene amplification and progressively elevated levels of tyrosine-phosphorylated p185erbB-2. (wikidata.org)
  • In research or diagnosis DNA amplification can be conducted through methods such as: Polymerase chain reaction, an easy, cheap, and reliable way to repeatedly replicate a focused segment of DNA by polymerizing nucleotides, a concept which is applicable to numerous fields in modern biology and related sciences. (wikipedia.org)
  • PCR 316 Gene Amplification Device merupakan salah satu instrument yang dirancang oleh brand Veri-Q untuk melakukan reaksi berantai Real-Time PCR secara cepat dan akurat mengamplifikasi asam nukleat target untuk diagnosis keberadaan virus. (andarupm.co.id)
  • Rápidas, Laboratório Estratégico, São with the Allplex amplification kit for SARS-CoV-2 diagnosis ( = 0.925). (bvsalud.org)
  • In the HER-2-negative clinical tumor samples, only 4/55 (7.3%) exhibited ABCC3 amplification. (lu.se)