• Aeromonas salmonicida is a pathogenic bacterium that severely impacts salmonid populations and other species. (wikipedia.org)
  • This is the first report of any bacterial species possessing two genes for homologs of this major outer membrane protein. (shengsci.com)
  • The bacterium is too similar to other Aeromonas species in its 16S rRNA gene sequence and does not contain suitably unique regions that could have been used to develop a species-specific FISH probe. (matis.is)
  • The fish pathogens A. hydrophila, A. salmonicida, E. tarda, Enterococcus seriolicida, Pasteurella piscicida, and Vibrio anguillarum are all widely distributed in fish farms in various areas, and within each species the R plasmid has an identical DNA sequence. (seafdec.org)
  • Identification of Aeromonas species is known to be troublesome due to their phenotypic and genotypic heterogeneity. (uludag.edu.tr)
  • The frequencies of the identified Aeromonas species were 38.33% for A. sobria, 23.33% for A. hydrophila, 10.0% for A. veronii and 26.67% for A. salmonicida and only one strain was not clearly identified. (uludag.edu.tr)
  • The isolate of A. salmonicida encodes a type three secretion system (TTSS) most likely located on a plasmid similar to pAsa5/pASvirA, which is known to specify one of the main virulence attributes of the species A. salmonicida. (unibe.ch)
  • Two of the nine bacteria isolated from several biotopes showed strong antibacterial activity against several pathogenic species such as Lactococcus garvieae , Vibrio anguillarum , Vibrio harveyi , Aeromonas hydrophila and Aeromonas salmonicida . (sciforum.net)
  • In T2S, GspA/B in Vibrio species and A. salmonicida (ExeA/B in A. hydrophila) has been found to be important for expression of the secretin. (microrna1.com)
  • Phylum barrier and Escherichia coli intra-species phylogeny drive the acquisition of antibiotic-resistance genes. (iame-research.center)
  • Enzyme activities and gene expression of a number of innate immune parameters in the serum, mucus and skin of Atlantic salmon (Salmo salar) were investigated after challenge with a pathogenic strain of Aeromonas salmonicida (A. salmonicida). (nih.gov)
  • The kinds of chemotherapeutants used are correlated with the occurrence of the corresponding drug-resistant genes in fish-pathogenic bacteria. (seafdec.org)
  • Take a look at check out friendships between the pathogenic bacterias Aeromonas salmonicida (The. (pf-8380inhibitor.com)
  • Antibacterial treatment of lumpfish (Cyclopterus lumpus) experimentally challenged with Vibrio anguillarum, atypical Aeromonas salmonicida and Pasteurella atlantica. (uib.no)
  • Citrobacter gillenii was isolated from the diseased fish and identified by biochemical and molecular methods including API 20E and 20NE and 16S rRNA gene region sequencing, respectively. (iranjournals.ir)
  • Here, we applied complementary omic approaches, including dual RNAseq and 16S rRNA gene metabarcoding sequencing using Nanopore and short-read Illumina technologies to unravel the host-pathogen interplay in an experimental infection system using the tropical fish Platax orbicularis as model. (biomedcentral.com)
  • The cat gene of P. piscicida was classified as CAT I, those of A. salmonicida and E. tarda were classified as CAT II, and that of V. anguillarum was classified as CAT II or IV, depending on the time the strains were isolated. (seafdec.org)
  • Salmon Trout Cyprinids Pike Perch Bullheads Turbot Halibut A. salmonicida, an airborne pathogen, can travel 104 cm from its host into the atmosphere and back to the water, thus making it difficult to control. (wikipedia.org)
  • 2021. Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes. (ulaval.ca)
  • ResFinderFG v2.0: a database of antibiotic resistance genes obtained by functional metagenomics. (iame-research.center)
  • Aeromonas salmonicida's ability to infect a variety of hosts, multiply, and adapt, make it a prime virulent bacterium. (wikipedia.org)
  • Systemic infection in European perch with thermoadapted virulent Aeromonas salmonicida (Perca fluviatilis). (unibe.ch)
  • Almost all multiple-drug resistant strains are carried on the transferable R plasmid, although resistance in fish pathogens to nitrofuran derivatives and pyridonecarboxylic acids is associated with a chromosomal gene. (seafdec.org)
  • The chloramphenicol resistance (cat) gene of the R plasmid from Gram-negative bacteria was classified into CAT I, II, III, and IV according to the DNA sequence. (seafdec.org)
  • By combining a balanced heterologous expression of (phenylpropanoid) naringenin biosynthetic pathway genes and the optimisation of yeast metabolism we developed a strain producing 430 mg/L of naringenin from glucose. (ruc.dk)
  • Aeromonas salmonicida is a Gram-negative, facultatively anaerobic, nonmotile bacterium. (wikipedia.org)
  • Fish were injected in the dorsal muscle with either 100 μl bacterium solution, about 3.05 × 10(7) CFU/ml A. salmonicida, or 100 μl 0.9% NaCl (as control group) and tissue samples were collected at days 0, 2, 4 and 6 post-injection. (nih.gov)
  • Crude cell-free supernatants of bacterial cultures of Aeromonas hydrophila, Aeromonas salmonicida, Pseudomonas aeruginosa, Pseudomonas fluorescens, Yersinia enterocolitica, and Serratia liquefaciens were screened for AHL production in selected ion monitoring mode, using the prominent fragment at m/z 143. (unibas.it)
  • salmonicida) and also Yersinia ruckeri (Ful. (pf-8380inhibitor.com)
  • A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. (shengsci.com)
  • This study shows that the 16S rDNA RFLP analysis combined with fstA gene specific PCR was useful for rapid and reliable identification of Aeromonas strains and the coupled determination of the virulence genes may help to both control fish disease arising from Aeromonas spp. (uludag.edu.tr)
  • However, the genes specifying the TTSS of the perch isolate show a higher temperature tolerance than strains isolated from cold-water fish. (unibe.ch)
  • The tested strains showed a great heterogeneity respect to their safety and antibiotic susceptibility and were taxonomically identified by partial 16S rDNA gene sequencing. (sciforum.net)
  • Rainbow trout have been found to carry A. salmonicida up to two years after initial infection without re-exposure. (wikipedia.org)
  • While observing chum salmon in a density of 14.7 fish per square meter, 12.4% were infected with A. salmonicida, whereas, densities at 4.9 fish per square meter were infection-free. (wikipedia.org)
  • At the end of the trial (133 days), in order to investigate the immunomodulatory properties of the phytogenic tested against a bacterial infection, an in vivo challenge with Aeromonas salmonicida was performed. (bvsalud.org)
  • However, little is known about the dynamic of infection in vivo, and information is lacking on both the intrinsic host response (gene expression) and its associated microbiota. (biomedcentral.com)
  • A total of 60 bacterial isolates from water and rainbow trout samples from Turkish coastal regions (Black Sea, Aegean Sea and Mediterranean Sea) were used for 16S rDNA-Restriction Fragment Length Polymorphisms (RFLP) analysis with AluI, MboI, NarI and PstI as restriction enzymes and molecular identification was eventually completed with PCR amplification of the fstA, A. salmonicida specific gene. (uludag.edu.tr)
  • A. salmonicida is an etiological agent for furunculosis, a disease that causes sepsis, haemorrhages, muscle lesions, inflammation of the lower intestine, spleen enlargement, and death in freshwater fish populations. (wikipedia.org)
  • Some clinical furunculosis survivors of an infected trout population became A. salmonicida carriers. (wikipedia.org)
  • When comparing furunculosis epidemics with depressed oxygen levels, when oxygen concentrations were decreased to less than 5 mg/L, A. salmonicida concentrations increased. (wikipedia.org)
  • In non-salmonid fish, Aeromonas salmonicidacan cause local infections with severe skin ulcerations, known as atypical furunculosis. (unibe.ch)
  • Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a. (shengsci.com)
  • Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli. (shengsci.com)
  • The Escherichia coli alkA Gene Is Activated to Alleviate Mutagenesis by an Oxidized Deoxynucleoside. (uib.no)
  • Here we identified a novel TAL, an enzyme from Aeromonas salmonicida with relatively high activity towards tyrosine and no activity towards phenylalanine. (ruc.dk)
  • These results indicate that the dietary supplementation of AQUOLIVE® at the level of 0.15% enhanced the systemic immune response and reduced the A. salmonicida cumulative mortality in Atlantic salmon smolts. (bvsalud.org)
  • 0.05), indicating the involvement of LSZ and AKP in the innate immunity of Atlantic salmon to A. salmonicida. (nih.gov)
  • So this study will not only provide a theoretical basis for vaccine development, but also provide basic data for the establishment of early warning systems for diseases caused by A. salmonicida in Atlantic salmon rearing. (nih.gov)
  • Markmið forverkefnisins var að búa til DNA þreifara sem binst við erfðaefni fisksjúkdómsvaldandi bakteríanna Flavobacterium psychrophilum og Aeromonas salmonicida,undirtegund achromogenes, sem hægt væri að skima eftir með notkun flúrljómunartækni í smásjá (FISH) og í örverugreini (flow cytometry). (matis.is)
  • A total number of 1,027 differentially expressed genes (DEGs) (805 up- and 222 downregulated) were found when comparing the transcriptomic profiling of the HK from fish fed the control and AQUOLIVE® diets. (bvsalud.org)
  • In resistant individuals, we detected up-regulation of specific immune-related genes differentiating resistant individuals from controls at 96 hpi, which suggests a possible genomic basis of resistance, although no genetic variation in coding regions was found. (biomedcentral.com)
  • The extracellular products of A. salmonicida consist of 25 proteins, enzymes, and toxins, and many more. (wikipedia.org)
  • The chromosome yields 58.5% of G+C pairs, has 4086 encoding proteins, and totals 4388 genes. (wikipedia.org)
  • Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. (shengsci.com)
  • I am interested in microbiome research, pharmacomicrobiomics, evolution and emergence microbial pathogenesis and resistance, microbial and phage genomics, antibiotic resistance surveillance, adaptive laboratory evolution, metabolic reconstruction and pathway gap filling, and virulence gene discovery. (peerj.com)
  • Ekki var hægt að búa til sértæka DNA þreifara fyrir A. Salmonicida,undirtegund achromogenes, þar sem auðkennisgen (16S rDNA) hennar er of líkt öðrum Aeromans tegundum sem eru ekki sýkjandi. (matis.is)
  • A. salmonicida isolates flourish when grown on blood agar or tyrosine. (wikipedia.org)
  • The proinflammatory cytokines TNF-α and IL-6 in lumpfish (Cyclopterus lumpus L.) -identification, molecular characterization, phylogeny and gene expression analyses. (uib.no)
  • Interleukin-1 ligands and receptors in lumpfish (Cyclopterus lumpus L.): molecular characterization, phylogeny, gene expression and transcriptome analyses. (uib.no)
  • Molecular cloning of MDA5, phylogenetic analysis of RIG-I-like receptors (RLRs) and differential gene expression of RLRs, interferons and proinflammatory cytokines after in vitro challenge with IPNV, ISAV and SAV in the salmonid cell line TO. (uib.no)
  • Molecular identification and determination of some virulence genes of Aeromonas spp. (uludag.edu.tr)
  • Additionally, A. salmonicida concentrations were considerably elevated in water with low dissolved oxygen (6-7 mg/L), compared to water with higher dissolved oxygen (10 mg/L). High density-low oxygen water resulted in survival rates that were roughly 40% less than in those consisting of low density-high oxygen conditions. (wikipedia.org)
  • Expression of ompAI and ompAII separately in E. coli indicated that both genes could be independently transcribed from their own promoters and that both gene products were assembled into the E. coli outer membrane. (shengsci.com)
  • The aim of the present study was to investigate the effects of prebiotic AMAX, probiotic Lactobacillus casei and combined AMAX and Lactobacillus casei on growth hormone (GH) and Insulin like Growth Factor (IGF1) genes expression in common carp. (aejournal.ir)
  • At the end of feeding trial, RNA extracted from liver and brain, cDNA synthesised using SuprimeScript RTase and growth genes expression were assessed using Real Time PCR. (aejournal.ir)
  • Acylated homoserine lactones (AHLs) are self-generated signal molecules that mediate population density-dependent gene expression (quorum sensing) in a variety of Gram-negative bacteria. (unibas.it)
  • Meanwhile, the mRNA levels of complement C3 (c3), igm and the genes associated with the TLR-NF-κB signaling pathway in the head kidney (HK) and spleen were also determined. (peroxidasessignaling.com)
  • Also, ampC, sul1 and floR resistance genes were detected in the isolated bacteria. (iranjournals.ir)
  • As an alternative approach we complemented the deletion of the TSC13 gene with homologues from various plants. (ruc.dk)
  • In addition, the presence of 6 virulence genes (aer, ser, lip, gcat, nuc and laf) in the isolates was investigated using rapid hexaplex-PCR. (uludag.edu.tr)
  • The more frequent virulence genes were aer, ser, lip and gcat found in 66.66%, 61.67%, 50% and 33.33% of isolates, respectively and 14 different virulence profiles were determined. (uludag.edu.tr)
  • Below a great mTERF gene V14 had been identified by fine mapping utilizing a depending albino mutant v14 that exhibited albinism simply in the first couple of correct results in, which has been validated by transgenic complementation assessments. (pf-8380inhibitor.com)