Gene addition localization of specific rnas. Medical search. Frequent questions

RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes. (wikipedia.org)
Each basic domain recognizes RNA, but many of these proteins require multiple copies of one of the many common domains to function. (wikipedia.org)
As nuclear RNA emerges from RNA polymerase, RNA transcripts are immediately covered with RNA-binding proteins that regulate every aspect of RNA metabolism and function including RNA biogenesis, maturation, transport, cellular localization and stability. (wikipedia.org)
These targets include mRNA, which codes for proteins, as well as a number of functional non-coding RNAs. (wikipedia.org)
Some binding proteins such as neuronal specific RNA-binding proteins, namely NOVA1, control the alternative splicing of a subset of hnRNA by recognizing and binding to a specific sequence in the RNA (YCAY where Y indicates pyrimidine, U or C). These proteins then recruit splicesomal proteins to this target site. (wikipedia.org)
Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including PRKRA, TRBP, and Dicer. (hindawi.com)
These proteins process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation. (hindawi.com)
Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
In addition, we determined that several nuage proteins localize to spermatogenic cell-specific structures, including mitochondria-associated granules (MAG), granulated bodies (GB), reticulated bodies (RB), and ribosome aggregates (RA) [ 22 - 26 ]. (hindawi.com)
Nine of these were uncharacterized proteins, and the remaining 16 proteins were analyzed by bioinformatics with respect to cell localization, tissue distribution, function, sequence motifs, three-dimensional structures and interaction networks. (scilifelab.se)
Several of the hits were eukaryotic-specific proteins interacting with DNA or RNA implying that Atox1 may act as a modulator of gene regulation. (scilifelab.se)
Many RNA binding proteins (RBPs) including the YTH family of proteins modulate the effects of m6A through specific binding to the methylated RNA. (diferencias-entre.org)
Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects. (degruyter.com)
In the central dogma RNA acts as a simple intermediary between the DNA that carries the genetic information and the proteins that define the whole variety of biological processes in the cell. (biomedcentral.com)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)

In addition, SUPPRESSOR OF GENE SILENCING 3 (SGS3), the RDR6-interacting protein 7 , exhibits phase separation both in vitro and in vivo through its prion-like domains, implicating the role of liquid-liquid phase separation in siRNA body formation. (nature.com)
These non-coding RNAs include microRNAs, small interfering RNAs (siRNA), as well as spliceosomal small nuclear RNAs (snRNA). (wikipedia.org)
Furthermore, siRNA knockdown of METTL16 resulted in expression changes of a few mRNA focuses on suggesting that METTL16 may play a role in regulating gene manifestation. (diferencias-entre.org)
We developed a generic automated eukaryotic short interfering RNA (siRNA) analysis tool, called RAPID. (uni-frankfurt.de)
Our tool captures diverse siRNA characteristics from small RNA sequencing data and provides easily navigable visualisations. (uni-frankfurt.de)
We also introduced a normalisation technique to facilitate comparison of multiple siRNA-based gene knockdown studies. (uni-frankfurt.de)
The protein is associated with microtubules , and Chatterjee and colleagues silenced the gene via short interfering RNA (siRNA) a molecular biological mechanism to study genes, targeting the CKAP5 encapsulated in lipid nanoparticles for in vivo delivery. (medicalxpress.com)

We predict several transcription factors to regulate differentially expressed genes between mLT-HSCs and other LT-HSC subsets. (biorxiv.org)
Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed genes (DEGs). (spandidos-publications.com)
Transcriptomic analysis on 72 h-exposed fetal testis explants revealed 187 differentially expressed genes (DEGs), including genes involved in steroid synthesis and toxic substance response. (biomedcentral.com)

OfGT3/42/46 were mainly localized in the nuclei and exhibited no transcriptional activities based on the analysis of the subcellular localization and transcriptional activity assay. (biomedcentral.com)
Additionally, the 5′ and 3′ UTRs flanking the coding region regulate mRNA translation, half-life, and subcellular localization [2]. (researchgate.net)
You can choose which resources to display for localization, including the Compartments subcellular localization database, the Human Protein Atlas and/or UniProt. (gene-info.org)

Baeg, K., Iwakawa, H. O. & Tomari, Y. The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing. (nature.com)
Alternative splicing is a mechanism by which different forms of mature mRNAs (messengers RNAs) are generated from the same gene. (wikipedia.org)
AceView: gene:dpy-21, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView. (nih.gov)
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
Alternative processing of nascent mRNAs is widespread in eukaryotic organisms and greatly impacts the output of gene expression. (stanford.edu)
In addition, changes in APA mark a variety of pathological states, including many types of cancer, in which mRNAs are preferentially cleaved at more proximal PASs, causing expression of mRNA isoforms with short 3'UTRs. (stanford.edu)
In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
Methyltransferase like -3 and -14 (METTL3 and METTL14) and Wilms tumor associating protein (WTAP) in addition to KIAA1429 are all components of the mRNA m6A methyltransferase complex, which uses a S-adenosyl methionine (SAM) binding website on METTL3 to methylate specific mRNAs for methylation having a RRACH m6A consensus sequence [10C15]. (diferencias-entre.org)
In addition, METTL16 offers been shown to bind and methylate mRNAs, including MAT2A, which can regulate its alternate splicing in response to cellular SAM Niraparib hydrochloride levels [29, 31, 32]. (diferencias-entre.org)
Although RNA editing has long been considered a relatively rare processing event, more recent research suggests that the vast majority of pre-mRNAs are edited [ 6 ]. (biomedcentral.com)

SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
Interestingly, exogenously overexpressed METTL16 differed from your endogenous protein in its relative affinity for RNA focuses on which prompted us to investigate METTL16's localization within the cell. (diferencias-entre.org)
Further, we developed a pipeline to characterise novel genome-wide endogenous short interfering RNAs (endo-siRNAs). (uni-frankfurt.de)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)

This protein functions through post-transcriptional modification of mRNA transcripts by changing the nucleotide content of the RNA. (wikipedia.org)
however, some protein-encoding RNA transcripts have been shown to be subject to editing resulting in a difference in their protein's amino acid sequence. (wikipedia.org)
No unspliced RHO transcripts could be detected in RNA from human retina. (molvis.org)
Using RT-PCR with degenerate primers on RNA from ovaries of hormone-stimulated immature rats, we identified transcripts for wnt-2 and wnt-2b. (elsevierpure.com)
The expression of wnt-2b transcripts in rat ovarian surface epithelium prompted us to examine whether the homologous gene is expressed in human ovarian cancer cell lines. (elsevierpure.com)
RT-PCR, using degenerate and specific primers for wnts, on RNA from five ovarian cancer cell lines confirmed the expression of transcripts for wnt-2b. (elsevierpure.com)
The localization of these transcripts in specific ovarian compartments suggests that this signal transduction pathway may be involved in follicular development and ovarian function. (elsevierpure.com)
In-depth analysis revealed that lncRNAs is an extensive and very heterogenic group from mRNA-like transcripts to circular RNAs. (biomedcentral.com)
A-to-I RNA editing is a post-transcriptional modification that converts adenosines to inosines in both coding and noncoding RNA transcripts. (biomedcentral.com)
Eukaryotic RNA transcripts can undergo a range of post-transcriptional modifications, which increase the diversity of the transcriptome without requiring increases in genome size. (biomedcentral.com)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)

Our data show that the stable knockdown of HIPK2 led to wtp53 misfolding, as detected by p53 immunoprecipitation with conformation-specific antibodies, and that p53 protein misfolding impaired p53 DNA binding and transcription of target genes. (aacrjournals.org)
We present evidence that zinc supplementation to HIPK2i cells increased p53 reactivity to conformation-sensitive PAb1620 (wild-type conformation) antibody and restored p53 sequence-specific DNA binding in vivo and transcription of target genes in response to Adriamycin treatment. (aacrjournals.org)
The receptors function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner. (embl.de)
MicroRNAs (miRNAs or miRs) are involved in the post-transcriptional regulation of gene expression via complete or partial base pairing with the 3′untranslated region of target genes ( 3 ). (spandidos-publications.com)

Specific mutations on those points may enable an avian influenza virus to become a human virus. (cdc.gov)
About half of cancers sustain mutations in the TP53 gene, whereas the other half maintain a wild-type p53 (wtp53) but may compromise the p53 response because of other alterations. (aacrjournals.org)
Missense mutations in the splicing factor gene PRPF31 cause a dominant form of retinitis pigmentosa (RP11) with reduced penetrance. (molvis.org)
In addition, intrinsic differences in subclonal tumor cells that arise from random mutations can create distinct niches within limited lesions. (spandidos-publications.com)
In primary tumors, mutations in driver genes frequently provide a survival advantage and give rise to a dominant clonal population. (spandidos-publications.com)
ADARs have been shown to contribute to disease pathologies by editing of glutamate receptors, editing of serotonin receptors, mutations in ADAR genes, and by other mechanisms, including recently identified regulatory roles in microRNA processing. (biomedcentral.com)
Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
[ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)

Messenger RNA (mRNA) vaccines represent a relatively new vaccine class showing great promise for the future. (researchgate.net)
Messenger RNA (mRNA) vaccines have progressed from a scepticism-inducing idea to clinical reality. (nanofcm.com)

Long noncoding RNAs (lncRNAs), due to their multifunction in various biological processes, have been indicated to play a crucial role in CSC renewal and stemness maintenance. (nature.com)
Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. (biomedcentral.com)
The second group includes rRNAs and long non-coding RNAs (lncRNAs), which to date are very poorly functionally annotated. (biomedcentral.com)
According to the GENCODE project one third of all human lncRNAs genes are primate-specific [ 4 ]. (biomedcentral.com)
For example, XIST (X-inactive specific transcript), one of the first described lncRNAs has a low level of sequence conservation, but a highly conserved function across placental mammals - inactivation of the X chromosome [ 5 ]. (biomedcentral.com)
Purpose: GG-H whole transcriptome array analysis suggested involvement of PININ (PNN) in the alternative splicing of multiple long non-coding RNAs (lncRNAs). (molvis.org)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
Alternatively spliced lncRNAs were detected with in situ hybridization with variant-specific RNA probes on human cornea sections. (molvis.org)
Conclusions: The data suggest PNN's role in the alternative splicing of a specific subset of lncRNAs might have a significant impact on the corneal epithelium. (molvis.org)

Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
Ovarian cadherins, in addition to acting as structural (adhesion) molecules, also function as modulators of gene activity. (elsevierpure.com)
Dual-luciferase reporter assay and RNA pull-down experiments were used to examine the mechanisms interaction between molecules. (biomedcentral.com)
On the one hand, recent and novel technologies produce biological data sets of ever-increasing resolution that reveal not only genomic sequences, but also RNA and protein abundances, their interactions with each other, their sub-cellular localization, and the identity and abundance of other biological molecules. (ucsd.edu)
Nucleic acid therapeutics hold promise in treating undruggable diseases and are recognized as the third major category of therapeutics in addition to small molecules and antibodies. (nanofcm.com)
In the case of RNA molecules that bind target RNA segments, such as microRNAs (miRNAs), the altered base pairing can change binding specificities. (biomedcentral.com)

Wang M, Yu F, Wu W, Zhang Y, Chang W, Ponnusamy M, Wang K, Li P. Circular RNAs: A novel type of non-coding RNA and their potential implications in antiviral immunity. (ijbs.com)
Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
Circular RNAs (circRNAs) are molecular sponges that are involved in regulation of multiple types of cancer. (spandidos-publications.com)
Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)

They especially play a major role in post-transcriptional control of RNAs, such as: splicing, polyadenylation, mRNA stabilization, mRNA localization and translation. (wikipedia.org)
Although RBPs have a crucial role in post-transcriptional regulation in gene expression, relatively few RBPs have been studied systematically.It has now become clear that RNA-RBP interactions play important roles in many biological processes among organisms. (wikipedia.org)
ncRNAs play important roles in controlling gene expression at both transcriptional and post-transcriptional levels. (ijbs.com)
A closely related debate around chromatin modifications is about causality: several histone PTMs correlate with specific transcriptional states, but in many cases they are not responsible for a transcriptional outcome but rather are a consequence of it [7] . (plos.org)
Lethality was associated with higher viral load and sustained innate immunity transcriptional signatures, whereas survival correlated with development of MARV GP-specific antibodies and early expression of predicted NK cell-, B-cell-, and cytotoxic T-cell-type quantities. (bvsalud.org)
RNA editing refers to post-transcriptional processes that alter the nucleotide sequence of an RNA transcript by insertion, deletion or nucleotide conversion. (biomedcentral.com)

Niraparib hydrochloride It can also bind and methylate the long noncoding RNAs MALAT1 and XIST [28, 30]. (diferencias-entre.org)

The most extensively studied form of RNA editing involves the ADAR protein. (wikipedia.org)
In mammals, the most prevalent form of RNA editing involves the conversion of adenosine to inosine (A-to-I) by hydrolytic deamination at the C6 position of adenine (Figure 1 a) [ 1 ]. (biomedcentral.com)

(12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)

Further, using RAPID, we investigated the aberrations of endo-siRNAs, and their respective transcriptomic alterations caused by an RNAi pathway triggered by feeding small RNAs against a target gene. (uni-frankfurt.de)

Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. (degruyter.com)
The effectiveness of mRNA treatments critically depends on chemical modifications and/or technologies designed to protect the nucleic acids from degradation and to ensure their stability in the circulation, to enable localization to the target tissue, and to ensure effective intracellular delivery. (nanofcm.com)

It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)

Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
ADAR3 contains an arginine-rich domain, shown in pink, which binds single-stranded RNA. (biomedcentral.com)

Many RBPs have modular structures and are composed of multiple repeats of just a few specific basic domains that often have limited sequences. (wikipedia.org)
Although many research efforts have focused on the molecular evolution of specific genes of influenza viruses, comprehensive comparisons among the nucleotide sequences of all 8 genomic segments and among the 11 encoded protein sequences have not been extensively reported. (cdc.gov)
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)

A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
After double digestion with gene nucleotide sequence and orientation. (thetechnoant.info)

There is growing need for a safe, efficient, specific and non-pathogenic means for delivery of nucleic acids. (nanofcm.com)
NanoFCM (Flow NanoAnalyzer) is a platform for comprehensive nanoparticle characterization, which covers the particle size range of 7-1000 nm, and allows the quantitative analysis of the particle size distribution, particle concentration, loading fraction, drug encapsulation efficiency and nucleic acid localization assay at the single particle level. (nanofcm.com)

Future studies will seek to confirm variations between cytoplasmic and nuclear RNA Niraparib hydrochloride focuses on in addition to exploring the physiological part of METTL16 through long-term knockdown. (diferencias-entre.org)

a P2X4 traveler gene and sEGFP displays apparent neuronal localisation but is apparently absent in microglia. (radarcon2008.org)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Eukaryotic cells express diverse RBPs with unique RNA-binding activity and protein-protein interaction. (wikipedia.org)
Diversity enabled eukaryotic cells to utilize RNA exons in various arrangements, giving rise to a unique RNP (ribonucleoprotein) for each RNA. (wikipedia.org)
RISC assembles into processing bodies (P-bodies) which are specific cytoplasmic foci in somatic eukaryotic cells [ 7 - 9 ]. (hindawi.com)

This process effectively changes the RNA sequence from that encoded by the genome and extends the diversity of the gene products. (wikipedia.org)
Studies of human genome demonstrate that protein-coding genes only occupy less than 2% of the entire genome [ 1 ]. (ijbs.com)
The genome of P. falciparum contains hundreds of genes that show CVGE, such that individual parasites within an isogenic population express these genes at very different levels, often fully active or completely silenced [15] . (plos.org)
In addition, OR expression pattern in various cancers were analyzed based on RNA-sequencing data reported in the Cancer Genome Atlas (TCGA), and the associations between patient survival outcomes and OR levels were analyzed to determine the clinical relevance and significances of OR expression in tumors. (bmbreports.org)
The transcriptomic and epigenomic regulatory landscape of the MAC genome, which has 80% protein-coding genes and short intergenic regions, is poorly understood. (uni-frankfurt.de)
Thousands of long non-coding RNA (lncRNA) genes are annotated in the human genome. (biomedcentral.com)
Achieving systematic, genome-scale perturbations within intact biological systems is important for elucidating gene function and epigenetic regulation. (cdc.gov)
To date, various genome-scale loss-of-function screening methods have been developed, including approaches employing RNA interference 1 , 2 and the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system 3 , 4 . (cdc.gov)

Notably, circRNAs display cell-type, tissue-type and developmental-stage specific expression patterns in eukaryotic transcriptome, which reveals their significant regulatory functions in gene expression. (ijbs.com)
More importantly, circRNAs serve as microRNA (miRNA) sponges and crucial regulators of gene expression. (ijbs.com)
In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. (stanford.edu)
Thus a set of spermatocyte-specific regulators choreograph the timing of expression of CycB protein during male meiotic prophase. (stanford.edu)
In this review, we summarize current understanding of how APA is regulated during development and cellular differentiation and how the resulting change in 3'UTR content affects multiple aspects of gene expression. (stanford.edu)
Clonally variant gene expression (CVGE) lies at the base of a bet-hedging adaptive strategy consisting of the stochastic generation of phenotypic diversity followed by natural selection upon environmental changes (for a recent discussion on bet-hedging, see [14] ). (plos.org)
The RNA-seq data suggested that the OfGT s have specific expression patterns in different tissues, and some were induced by salt stress. (biomedcentral.com)
In addition, OfGT42/OfGT46 had a co-expression pattern under salt-stress conditions. (biomedcentral.com)
Remarkably, the transient transformation results showed lower MDA content and increased expression of ROS-related genes NbAPX in transgenic plants, which implying OfGT3/42/46 may improve the salt tolerance of tobacco. (biomedcentral.com)
This study made a comprehensive summary of the OfGT gene family, including functions and co-expression patterns in response to salt and other stresses, as well as an evolutionary perspective. (biomedcentral.com)
In many cases, the disease gene encodes a component of phototransduction or allied processes with expression confined to the retina, for example the rod visual pigment (reviewed in [ 1 ]), or peripherin-RDS [ 2 ], but not all RP genes show such a restricted pattern of expression. (molvis.org)
In addition, the clinical relevance and significance of the levels of OR expression were evaluated. (bmbreports.org)
Our analysis revealed subsets of genes, whose expression is positively associated with H3K27me3, different to the otherwise reported negative association with gene expression in many other organisms. (uni-frankfurt.de)
Further, we developed a Random Forests classifier to predict gene expression using genic (gene length, intron frequency, etc.) and epigenetic features. (uni-frankfurt.de)
Upon evaluating different feature sets, we found that genic features are as predictive, of gene expression, as the epigenetic features. (uni-frankfurt.de)
We used Shapley local feature explanation values, to suggest that high H3K4me3, high intron frequency, low gene length, high sRNA, and high GC content are the most important elements for determining gene expression status. (uni-frankfurt.de)
qRT-PCR and western blot were performed to detect the gene and protein expression. (biomedcentral.com)
For instance, Human Protein Atlas localization and expression information is only available for human gene entries. (gene-info.org)
The GO term, Localization, Protein expression and RNA expression sections have advanced display options that can be viewed by clicking the settings/ellipsis button ( ) next to the relevant field in the extension popup. (gene-info.org)
Generally, lncRNA has a lower expression level, their expression is more tissue-specific, and the majority of lncRNA has nuclear localization. (biomedcentral.com)
FUT6 and CGNL1 were downregulated in HNSCC compared with adjacent healthy tissue and the expression levels of these genes were associated with tumor stage. (spandidos-publications.com)
thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)
Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. (cdc.gov)
The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)
[ 7 ] In addition, recent gene expression profiling studies support the concept that radial glial cells from different neuroanatomic sites may be predisposed to acquiring particular genetic aberrations that result in ependymomas with site-specific genetic signatures and biologic potential. (medscape.com)
tures and gene expression patterns. (lu.se)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
the gene expression profile during B lymphoid differentiation. (lu.se)
This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)

APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)

Typing for influenza A virus was then performed with immunofluorescent assay by type-specific monoclonal antibody (Dako, Cambridgeshire, UK). (cdc.gov)

This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)
The dot-ELISA was carried out as explained by Wu gene sequence with 783 nucleotides was PCR-amplified. (thetechnoant.info)

The study of PSCs has relied on an anatomy-based approach, as the identities of cell-specific PSC molecular markers have remained elusive. (elifesciences.org)
Using a newly generated transgenic mouse in which PSCs are specifically labeled, we show that PSCs have a unique molecular signature that includes genes known to play critical roles in PSCs and synapses. (elifesciences.org)
This has been due to the absence of a cell-specific molecular marker with which PSCs can be identified, isolated, and genetically manipulated. (elifesciences.org)
Specific molecular probes and primers are designed for this purpose. (enzolifesciences.com)
DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)
In addition, rapidly increasing capabilities of rapid molecular and genomic analyses in the clinic promise to transform medical practice in unprecedented ways. (ucsd.edu)
Thus, further investigations into the specific molecular mechanisms underlying circRNA/miRNA/mRNA regulation are required to aid discovery of effective therapeutic targets. (spandidos-publications.com)

Therefore, while METTL16 has been reported to be a nuclear protein, our findings suggest that METTL16 is also a cytoplasmic methyltransferase that may alter its RNA binding Niraparib hydrochloride preferences depending on its cellular localization. (diferencias-entre.org)

RBPs have crucial roles in various cellular processes such as: cellular function, transport and localization. (wikipedia.org)
APA naturally occurs during development and cellular differentiation, with around 70% of human genes displaying APA in particular tissues and cell types. (stanford.edu)

The results implied that the OfGT genes were related to abiotic and hormonal stress responses in O. fragrans , and that the OfGT3/42/46 genes in particular might play crucial roles in responses to salt stress. (biomedcentral.com)
Identification of New Potential Interaction Partners for Human Cytoplasmic Copper Chaperone Atox1: Roles in Gene Regulation? (scilifelab.se)
In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)

While global studies have recognized many potential RNA focuses on of METTL16, only a handful, including the long noncoding RNA MALAT1, the snRNA U6, as well as the mRNA MAT2A have been verified and/or analyzed to any great degree. (diferencias-entre.org)

Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)

The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)

However, METTL16 (METT10D) has also been identified as an RNA m6A methyltransferase that can methylate both coding and noncoding RNAs, but its biological role remains unclear. (diferencias-entre.org)
Stochastic Optical Reconstruction Microscopy, STORM , is one of a family of Nobel Prize winning super-resolution Single Molecule Localization Microscopies ( SMLM ) for the visualization of biological systems with an optical resolution measured in the tens of nanometers (nm) in the x, y, and z directions. (microscopyu.com)

Unlike linear RNAs, circRNAs form covalent-closed continuous loops without 5' to 3' polarities and poly (A) tails. (ijbs.com)
CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)

Here, we show, by microarray analysis, that wtp53 lost the target gene activation following stable knockdown of HIPK2 (HIPK2i) in colon cancer cell line. (aacrjournals.org)
Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)

The OfGT s were found to be located randomly on the 20 O. fragrans chromosomes, and an analysis of gene replication events indicated that the OfGT gene family underwent strong purification selection during the evolutionary process. (biomedcentral.com)
Currently, a range of probes can be synthesized to locate and quantify specific short RNAs, genes, entire chromosomes, and even cells (Evanko 2007 ). (springer.com)

Epigenetic reprogramming and small RNA silencing of transposable elements in pollen. (nature.com)

However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
We find that the small RNA transcriptome is altered, even if a gene unrelated to RNAi pathway is targeted. (uni-frankfurt.de)

RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others. (wikipedia.org)
The analysis of conserved motifs and gene structures implied that the OfGT members in the same subfamily have similar conserved motifs and gene structures. (biomedcentral.com)

Many of these signatures are found in NP, PA, and PB2 genes (viral ribonucleoproteins [RNPs]) and are mostly located in the functional domains related to RNP-RNP interactions that are important for viral replication. (cdc.gov)
Consequently, it lays a foundation for further functional characterizations of these genes. (biomedcentral.com)
In addition, an in-depth understanding of the functional effect of diet is lacking [ 1 ] [ 2 ] . (encyclopedia.pub)
The systematically described collection of microbes is known as the microbiota, while the term microbiome includes their pool of functional genes. (encyclopedia.pub)
Physical and functional interaction networks chart connectivities, reveal functional modules, and provide clues on the functioning of specific genes. (ucsd.edu)

In Drosophila males, meiotic prophase lasts 3.5 days, during which spermatocytes upregulate of over 1800 genes and grow 25-fold. (stanford.edu)

We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)

however, their specific recognition and the selective processing of siRNAs remain elusive. (nature.com)

All RBPs bind RNA, however they do so with different RNA-sequence specificities and affinities, which allows the RBPs to be as diverse as their targets and functions. (wikipedia.org)
The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
Not only do choroid plexus tumors and ependymomas (including the various histologic subtypes) clearly recapitulate specific cell types found at various stages in this ontologic sequence, so too do a variety of other uncommon and/or recently recognized entities. (medscape.com)

NcRNAs almost always function as ribonucleoprotein complexes and not as naked RNAs. (wikipedia.org)

During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. (stanford.edu)
In the last two decades, our understanding of human gene regulation has improved tremendously. (uni-frankfurt.de)

In addition, on average lncRNA are shorter than mRNA. (biomedcentral.com)

Such tumor-specific T RM cells are recognized as both necessary and sufficient for long-lived protection against tumors in peripheral tissue locations. (frontiersin.org)

Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

More importantly, we summarize the potential of various RNA-based therapeutics clinically available for cancer treatment. (biomedcentral.com)
RNA-dependent targeting of the TIME, as monotherapy or combined with other evolving therapeutics, might be beneficial for cancer patients' treatment in the near future. (biomedcentral.com)

Other than core splicesome complex, RBPs also bind to the sites of Cis-acting RNA elements that influence exons inclusion or exclusion during splicing. (wikipedia.org)

Similar SMLM techniques include Photoactivated Localization Microscopy ( PALM) and Ground State Depletion Individual Molecule Return ( GSDIM) , among many others. (microscopyu.com)

TIME-related RNA or RNA regulators could be promising targets for anticancer immunotherapy. (biomedcentral.com)

As a result a specific signal is produced, indicating an interaction has taken place. (genomicglossaries.com)

The outcomes highlighted the importance of the gene of interest as a therapeutic target to investigate genetically unstable ovarian cancers to further elucidate its mechanisms of action. (medicalxpress.com)

What are the Differences Between DNA and RNA Probes? (enzolifesciences.com)

Here, we suggest that plant transposon RNAs undergo frequent ribosome stalling caused by their unfavourable codon usage. (nature.com)

However, since most mature RNA is exported from the nucleus relatively quickly, most RBPs in the nucleus exist as complexes of protein and pre-mRNA called heterogeneous ribonucleoprotein particles (hnRNPs). (wikipedia.org)
According to the Eukaryotic RBP Database (EuRBPDB), there are 2961 genes encoding RBPs in humans. (wikipedia.org)

We also predicted phasing of siRNAs, which are regulated by the RNA interference (RNAi) pathway. (uni-frankfurt.de)

Position-specific entropy profiles created from scanning 306 human and 95 avian influenza A viral genomes showed that 228 of 4,591 amino acid residues yielded significant differences between these 2 viruses. (cdc.gov)
In this study, we used several computational approaches for finding specific genetic signatures characteristic of human and avian influenza A viral genomes. (cdc.gov)
Here, we review recent studies investigating connections between ADAR-mediated RNA editing and human diseases. (biomedcentral.com)

Subtyping was conducted by reverse transcription (RT)-PCR with subtype-specific primers. (cdc.gov)

Anatomy9

Chemicals and Drugs15

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Psychiatry and Psychology1

Phenomena and Processes18

Disciplines and Occupations1

Information Science3

Proteins19

SiRNA7

Differentially expressed genes3

Subcellular localization3

MRNAs10

Endogenous6

Transcripts11

Target genes4

Mutations8

Messenger RNA2

LncRNAs9

Molecules6

Circular RNAs5

Transcriptional6

Long nonc1

Form of RNA editing2

MicroRNAs1

Endo-siRNAs1

Intracellular2

Hybridization3

Single stranded2

Sequences4

Nucleotide sequence2

Nucleic2

Cytoplasmic and nuclear1

Neuronal2

Eukaryotic cells3

Genome8

Expression29

MRNA translation1

Assay1

Nucleotides2

Molecular8

Nuclear1

Cellular2

Roles3

SnRNA1

POLYMERASE1

Ovarian cancer cell1

Biological2

CircRNAs2

Knockdown2

Chromosomes2

Epigenetic1

Transcriptome2

Motifs2

Functional5

Upregulate1

Isoforms2

Processing of siRNAs1

Sequence3

NcRNAs1

Regulation2

LncRNA1

Tissue1

Differentiation1

Therapeutics2

Exons1

Microscopy1

Regulators1

Interaction1

Mechanisms1

Probes1

Undergo1

RBPs2

Interference1

Human3

Primers1