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Chromatography, AffinityGelsChromatography, High Pressure LiquidChromatography, GelChromatography, Ion ExchangeSepharoseMolecular Sequence DataChromatographyMolecular WeightElectrophoresis, Polyacrylamide GelAmino Acid SequenceKineticsChromatography, GasProtein BindingChromatography, LiquidChromatography, Thin LayerElectrophoresis, Gel, Two-DimensionalBinding SitesSubstrate SpecificityChromatography, DEAE-CelluloseBase SequenceEscherichia coliRecombinant ProteinsAffinity LabelsChromatography, AgaroseHydrogen-Ion ConcentrationLectinsIsoelectric FocusingAmino AcidsBinding, CompetitiveRabbitsCattleCloning, MolecularMass SpectrometryAntibody AffinityPeptide FragmentsCarrier ProteinsElectrophoresis, Gel, Pulsed-FieldElectrophoresis, Agar GelCell LineCarbohydratesMacromolecular SubstancesPlant LectinsTrypsinBacterial ProteinsSequence Homology, Amino AcidPeptidesChemistryRecombinant Fusion ProteinsLigandsChemical PhenomenaGlycoproteinsLiverCell MembraneImmunodiffusionTemperatureIsoelectric PointProtein ConformationDNASolubilityAntibodies, MonoclonalStructure-Activity RelationshipPolysaccharidesCarbohydrate SequenceOligosaccharidesSwineHydrolysisHeparinBlotting, WesternChemical FractionationElectrophoresisHemagglutinationAntibodiesIsoenzymesModels, MolecularGas Chromatography-Mass SpectrometryCells, CulturedMembrane ProteinsReceptors, Cell SurfaceProteinsMutationSpecies SpecificityImmune SeraProtein Structure, TertiaryImmunoelectrophoresisDetergentsMethodsGlycopeptidesTandem Mass SpectrometryCross-Linking ReagentsDrug StabilityPlasmidsCentrifugation, Density GradientSpectrophotometry, UltravioletRats, Inbred StrainsChromatography, PaperGlycosylationChickensImmunoblottingCross Reactions

ElectrophoresisAgarose GelMolecularHysteresisPowderStrengthPointTechniquesMonoclonalTissue cultureRecombinantPurityProteinReceptorCells

Electrophoresis4

• Agarose is often used in biochemistry laboratories as a semi-solid support in techniques such as electrophoresis and chromatography for the separation and analysis of biological macromolecules or small molecules. (ginobiotech.com)
• Simply mix with water, gently heat to dissolve the powder blend to quickly prepare reliable agarose gels for electrophoresis. (sigmaaldrich.com)
• Agarose is non-toxic and has several properties and specifications that make it useful as a gelling agent in many applications, such as nucleic acid electrophoresis, immunodiffusion techniques, gel plates or overlays for cells in tissue culture, cell culture media, gel chromatography, affinity chromatography, and ion exchange chromatography. (sigmaaldrich.com)
• Gel electrophoresis is a common life science laboratory technique used to extract biological molecules based on their size, such as in DNA separation and DNA detection. (sigmaaldrich.com)

Agarose Gel1

• The anionic groups of the agarose gel are attached to the matrix and cannot migrate, while the dissociable countercations migrate to the cathode within the matrix to generate EEO. (sigmaaldrich.com)

Molecular2

• The molecular weight was estimated at 400,000 daltons by calibrated gel filtration, and the pI estimated at 5.7-6.4 by isoelectric focusing. (lsu.edu)
• At least two subunits having molecular weights of 112,000 and 40,000 daltons, respectively, were identified by calibrated SDS-polyacrylamide gels. (lsu.edu)

Hysteresis1

• Agarose solutions exhibit hysteresis when transitioning from liquid to gel. (sigmaaldrich.com)

Powder1

• Agarose is an organic substance with the chemical formula C24H38O19, a white or yellow bead-like gel particle or powder, which is a linear polymorph. (ginobiotech.com)

Strength2

• The vitellogenin was partially purified and isolated by low-ionic-strength precipitation and DEAE-affinity chromatography. (lsu.edu)
• Gel strength - The force that must be applied to the gel to fracture it. (sigmaaldrich.com)

Point2

• Gel point - The temperature at which an aqueous solution of agarose forms a gel upon cooling. (sigmaaldrich.com)
• In other words, the gel point is not the same as the melting temperature. (sigmaaldrich.com)

Techniques1

• The present affinity results are compared with corresponding studies with other aminotransferases in an attempt to find possible universal techniques for their purification. (eurekamag.com)

Monoclonal2

• Monoclonal antibodies were raised against purified bovine and human brain G-proteins. (researchgate.net)
• The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

Tissue culture1

• Agarose is non-toxic and has several properties and specifications that make it useful as a gelling agent in many applications, such as nucleic acid electrophoresis, immunodiffusion techniques, gel plates or overlays for cells in tissue culture, cell culture media, gel chromatography, affinity chromatography, and ion exchange chromatography. (sigmaaldrich.com)

Recombinant2

• Through molecular biology techniques, the pentapeptide sequence, molecular weight, and architecture of the ELP can be precisely controlled for subsequent an purified as a recombinant polypeptide, resulting in monodisperse, non-immunogenic (Urry et al. (justia.com)
• To characterize the biochemical properties of JEV RdRp, we expressed in Escherichia coli and purified an enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus. (biomedcentral.com)

Purity1

• NS3 and NS5A were over-expressed and using Nickel-affinity method both proteins were purified to ~ 95% purity. (biomedcentral.com)

Protein3

• Further, we purified Ct CHI1 protein from E. coli which can effectively catalyze isomerization of 2′,4′,4,6′-tetrahydroxychalcone into naringenin in vitro. (biomedcentral.com)
• The purified NS5 protein, but not the mutant NS5 protein with an Ala substitution at the first Asp of the RdRp-conserved GDD motif, exhibited template- and primer-dependent RNA synthesis activity using a poly(A) RNA template. (biomedcentral.com)
• The HHMI-funded Kodak digital imager (below) was utilized during both the protein purification (to visualize SDS-PAGE gels) and the restriction digestion stages of the course. (carleton.edu)

Receptor2

• Affinity to the Fc receptor CD16 on NK cells can be increased by genetically engineering the glycosylation motifs or the amino-acid sequence of the Fc part. (frontiersin.org)
• The invention also relates to uses for the purified hormone receptor molecule. (everypatent.com)

Cells1

• We here used a B7-H3 mAb to generate chimeric mAbs containing either a wildtype Fc-part (8H8_WT) or a variant Fc part with amino-acid substitutions (S239D/I332E) to increase affinity for CD16 expressing NK cells (8H8_SDIE). (frontiersin.org)