Filter...

AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care

Select a category...

Chromatography, High Pressure LiquidGelsChromatography, LiquidChromatography, AffinityChromatography, GasChromatography, GelChromatographyChromatography, Ion ExchangeChromatography, Thin LayerMass SpectrometryMolecular Sequence DataAmino Acid SequenceChromatography, Reverse-PhaseMolecular WeightBacterial ProteinsTandem Mass SpectrometryKineticsIonic LiquidsElectrophoresis, Polyacrylamide GelEscherichia coliChromatography, DEAE-CelluloseGas Chromatography-Mass SpectrometryGene Expression Regulation, BacterialElectrophoresis, Gel, Two-DimensionalHydrogen-Ion ConcentrationSpectrophotometry, UltravioletSpectrometry, Mass, Electrospray IonizationCulture MediaAmino AcidsBase SequenceChromatography, AgaroseCountercurrent DistributionSigma FactorStereoisomerismTemperatureSubstrate SpecificityDrug StabilityReproducibility of ResultsCalibrationIndicators and ReagentsReference StandardsMutationChemical FractionationIsoelectric FocusingChromatography, PaperCapillary ElectrochromatographyAcetonitrilesCattleGenes, BacterialMagnetic Resonance SpectroscopyTrypsinElectrophoresis, Gel, Pulsed-FieldLimit of DetectionPeptide FragmentsChemistryElectrophoresis, Agar GelSepharoseChemical PhenomenaCloning, MolecularPeptidesLiquid CrystalsTime FactorsSolid Phase ExtractionHydrolysisRabbitsHot TemperatureProtein BindingDNA, BacterialLiverMethodsRNA, BacterialEscherichia coli ProteinsTranscription, GeneticCarbohydratesMolecular StructureSaccharomyces cerevisiaeSilicon DioxideSolventsRecombinant ProteinsMicrochemistryIsomerismSensitivity and SpecificityPlant ExtractsProteomicsCarbohydrate SequenceMethanolSpecies SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBacillus subtilisProteomeChromatography, Micellar Electrokinetic CapillaryOligosaccharidesRadioimmunoassayBinding SitesSequence Homology, Amino AcidSilica GelPlasmidsBiotransformationCell LineFatty Acids

Column chromatographyLayer chromatographyExchange chromatographyPreparativeSilicaAffinityAnalyticalHPLCChromatographic techniquesAdsorbentMixturesSeparation techniqueSolventCompoundsSolubilityMechanismAnalyteSubstancesOrganicMobile phaseColumnsWidelySeparateMethodHighProcessPowerfulSystemsSizeMediumTypesSampleTime

Column chromatography15

• A chemist in the 1950s using column chromatography. (blogspot.com)
• Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. (blogspot.com)
• The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. (blogspot.com)
• The stationary phase or adsorbent in column chromatography is a solid. (blogspot.com)
• The most common stationary phase for column chromatography is silica gel , followed by alumina . (blogspot.com)
• For silica column chromatography, this ratio lies within 20:1 to 100:1, depending on how close to each other the analyte components are being eluted. (blogspot.com)
• How Do You Prepare Column Chromatography? (chromatographytoday.com)
• How Does Column Diameter Affect Column Chromatography? (chromatographytoday.com)
• In previous days, Column chromatography was used in many laboratories for preparative purposes as well as for reaction control in organic synthesis. (ijpsr.com)
• Column chromatography is an extremely time consuming stage in any lab and can quickly become the bottleneck for any process lab. (ijpsr.com)
• Column chromatography has found its place in many laboratories for preparative purposes as well as for reaction control in organic syntheses. (ijpsr.com)
• Column chromatography is separated into two categories, depending on how the solvent flows down the column. (ijpsr.com)
• If the solvent is allowed to flow down the column by gravity, or percolation, it is called gravity column chromatography. (ijpsr.com)
• In traditional column chromatography a sample to be purified is placed on the top of a column containing some solid support, often silica gel. (ijpsr.com)
• Flash chromatography is basically an air pressure driven hybrid of medium pressure and shorter column chromatography which has been optimized for particularly rapid separation. (ijpsr.com)

Layer chromatography1

• The eluent is optimized in small scale pretests, often using thin layer chromatography (TLC) with the same stationary phase. (blogspot.com)

Exchange chromatography3

• Also possible are ion exchange chromatography , reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). (blogspot.com)
• A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (lookformedical.com)
• Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)

Preparative3

• The classical preparative chromatography column, is a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter (a glass frit or glass wool plug - to prevent the loss of the stationary phase) at the bottom. (blogspot.com)
• This leads to the development of novel preparative liquid chromatography in which mobile phase flows down by positive air pressure called as Flash chromatography. (ijpsr.com)
• It is a simple, fast and economical approach to preparative Liquid chromatography .This review try to focus on principle, various components, general procedure, advantages and application of Flash chromatography. (ijpsr.com)

Silica2

• The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
• Flash chromatography differs from the conventional technique in two ways: first, slightly smaller silica gel particles (250-400 mesh) are used, and second, due to restricted flow of solvent caused by the small gel particles, pressurized gas ( ca. 10-15 psi) is used to drive the solvent through the column of stationary phase. (ijpsr.com)

Affinity1

• Chromatography is essentially a separation technique - the sample is carried along by a mobile phase (either gas or liquid) and the components of the sample mixture are separated due to their affinity for the stationary phase. (chromatographytoday.com)

Analytical2

• The composition of the eluent flow can be monitored and each fraction is analyzed for dissolved compounds, e.g. by analytical chromatography, UV absorption, or fluorescence . (blogspot.com)
• Along with spectroscopy, chromatography is one of the major analytical techniques at the disposal of any chemical or biochemical researcher. (chromatographytoday.com)

HPLC3

• Automated flash chromatography systems include components normally found on more expensive HPLC systems such as a gradient pump, sample injection ports, a UV detector and a fraction collector to collect the eluent. (ijpsr.com)
• A theoretical description of analyte retention in high-performance liquid chromatography (HPLC) has been the subject of various publications. (springer.com)
• Hydrophilic interaction liquid chromatography (HILIC) is an alternative high-performance liquid chromatography (HPLC) mode for separating polar compounds. (springer.com)

Chromatographic techniques3

• Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (lookformedical.com)
• Chromatographic techniques in which the mobile phase is a liquid. (lookformedical.com)
• There are many different chromatographic techniques - from the relatively simple paper chromatography used in primary schools to separate ink spots - to the complex LC/MS/MS techniques used in advanced research to identify drug metabolites. (chromatographytoday.com)

Adsorbent1

• Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase). (lookformedical.com)

Mixtures2

• Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. (lookformedical.com)
• Flash chromatography is a technique used to separate mixtures of molecules into their individual constituents, frequently used in the drug discovery process 1 . (ijpsr.com)

Separation technique1

• Separation technique in which the stationary phase consists of ion exchange resins. (lookformedical.com)

Solvent3

• The mobile phase or eluent is either a pure solvent or a mixture of different solvents. (blogspot.com)
• If the solvent is forced down the column by positive air pressure, it is called flash chromatography, a "state of the art" method currently used in organic chemistry research laboratories 1 . (ijpsr.com)
• In flash chromatography however air pressure is used to speed up the flow of solvent, dramatically decreasing the time needed to purify the sample, therefore making the column and running the separation could take less than 10-15 minutes. (ijpsr.com)

Compounds7

• A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds. (lookformedical.com)
• A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
• Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
• The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. (springer.com)
• NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
• For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
• A desirable mobile phase would contain high organic content for better sensitivity and also show good on-column retention for polar ionic compounds. (springer.com)

Solubility1

• Polar samples always show good solubility in the aqueous mobile phase used in HILIC, which overcomes the drawbacks of the poor solubility often encountered in NP-LC. (springer.com)

Mechanism2

• Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
• For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)

Analyte1

• There is an important ratio between the stationary phase weight and the dry weight of the analyte mixture that can be applied onto the column. (blogspot.com)

Substances1

• HILIC also allows the analysis of charged substances, as in ion chromatography (IC). (springer.com)

Organic1

• A solution of the organic material is pipetted on top of the stationary phase. (blogspot.com)

Mobile phase5

• For the dry method, the column is first filled with dry stationary phase powder, followed by the addition of mobile phase, which is flushed through the column until it is completely wet, and from this point is never allowed to run dry. (blogspot.com)
• A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
• The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. (springer.com)
• In normal phase liquid chromatography (NP-LC), the stationary phase is more polar than the mobile phase. (springer.com)
• The retention increases as the polarity of the mobile phase decreases, and thus polar analytes are more strongly retained than nonpolar ones. (springer.com)

Columns2

• The productivity of chromatography can be increased by running several columns at a time. (blogspot.com)
• Chromatography on thin layers of adsorbents rather than in columns. (lookformedical.com)

Widely1

• They are widely used in foodstuffs as gelling agents and a source of fibre. (chromatographytoday.com)

Separate1

• The individual components are retained by the stationary phase differently and separate from each other while they are running at different speeds through the column with the eluent. (blogspot.com)

Method1

• For the wet method, a slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. (blogspot.com)

High1

• The net result is a rapid ("over in a flash") and high resolution chromatography. (ijpsr.com)

Process1

• During the entire chromatography process the eluent is collected in a series of fractions . (blogspot.com)

Powerful1

• Supercritical Fluid Chromatography (SFC): A Powerful. (chromatographytoday.com)

Systems2

• Several manufacturers have developed automated flash chromatography systems. (ijpsr.com)
• MPLC - Medium pressure liquid chromatography (MPLC) systems which operate above 150 psi. (ijpsr.com)

Size1

• Katerina used size exclusion chromatography (SEC) to investigate okra's benefits as an emulsifier in the food industry. (chromatographytoday.com)

Medium1

• Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)

Types1

• Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)

Sample1

• Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (lookformedical.com)

Time1

• It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. (blogspot.com)