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  • affinity
  • Other desirable properties of the gel forming agent are the absence of ionizing groups and, in a given solvent, low affinity for the substances to be separated. (wikipedia.org)
  • appropriate
  • When an appropriate gel filtration resin is used, many different classes of macromolecules can be separated from buffer salts, unconjugated labeling reagents and other molecules to achieve rapid purification before downstream applications. (thermofisher.com)
  • back
  • The term gel permeation chromatography can be traced back to J.C. Moore of the Dow Chemical Company who investigated the technique in 1964 and the proprietary column technology was licensed to Waters Corporation, who subsequently commercialized this technology in 1964. (wikipedia.org)
  • column
  • The column is filled with the gel. (wikipedia.org)
  • Finally, in contrast to dialysis, gel filtration chromatography allows the contaminating material to be removed in a relatively small volume (and left on the column), an important feature when working with toxic or radioactive substances. (thermofisher.com)
  • When a sample solution is passed through a column of packed gel filtration resin, small molecules in the sample (buffer salts, small molecules, etc.) enter the pores of resin beads that they encounter and are forced to follow a circuitous path before later exiting the beads. (thermofisher.com)