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GelsElectrophoresis, Polyacrylamide GelMolecular WeightElectrophoresis, Gel, Two-DimensionalElectrophoresisElectrophoresis, CapillaryElectrophoresis, Agar GelElectrophoresis, Gel, Pulsed-FieldIsoelectric FocusingElectrophoresis, DiscChromatography, GelAmino AcidsKineticsMolecular Sequence DataIsoelectric PointSodium Dodecyl SulfateChromatography, AffinityImmunodiffusionChromatography, Ion ExchangeAmino Acid SequenceHydrogen-Ion ConcentrationBase SequenceImmunoelectrophoresisChromatography, DEAE-CelluloseAcrylic ResinsElectrophoresis, Starch GelMacromolecular SubstancesBlood Protein ElectrophoresisElectrophoresis, MicrochipEscherichia coliChromatographyBacterial ProteinsSubstrate SpecificityCentrifugation, Density GradientRabbitsChemistryGlycoproteinsChemical PhenomenaCattleElectrophoresis, Cellulose AcetateCarbohydratesChemical PrecipitationSpecies SpecificityTrypsinPeptidesProteinsDNA, BacterialPeptide FragmentsDenaturing Gradient Gel ElectrophoresisDensitometryDNAAcrylamidesTemperatureAcrylatesImmunochemistryLiverProtein BiosynthesisCell LineImmunoelectrophoresis, Two-DimensionalUltracentrifugationHot TemperatureViral ProteinsPolymerase Chain ReactionMicroscopy, ElectronImmune SeraMembrane ProteinsCell MembraneCell FractionationHydroxyapatitesCell-Free SystemChromatography, High Pressure LiquidIsoenzymesProtein BindingCloning, MolecularMethodsProteomicsTritiumImmunosorbent TechniquesPeptide HydrolasesAutoradiographyMutationPeptide BiosynthesisSepharoseSwineNucleic Acid DenaturationBlood ProteinsCross ReactionsAmmonium SulfateProteomeChickensNative Polyacrylamide Gel ElectrophoresisRosaniline DyesDetergentsAffinity LabelsMercaptoethanolSolubilityBacterial Typing TechniquesNucleic Acid HybridizationPlasmidsImmunologic Techniques
ProteinsAgarose gel electrophorNucleic Acid ElectrophoresisPrecast gelsProteinSodiumEletroforese em gel de poliacrilamidaMolecular weightPrimersGradient gelAcrylamideMembranesCompositionAnalyzeBufferNativeDetectionMembraneHorizontalPAGEImmuneStripsPoresMethodCellsBiological systemsDryersProductsProduct lineComplexReal-timeEquipmentIncludesBandArticles
Proteins21
  • Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. (wikipedia.org)
  • At the pH at which gel electrophoresis is carried out the SDS molecules are negatively charged and bind to proteins in a set ratio, approximately one molecule of SDS for every 2 amino acids. (wikipedia.org)
  • By comparing the relative ratio of the distance traveled by each protein to the length of the gel (Rf) one can make conclusions about the relative molecular weight of the proteins, where the length of the gel is determined by the distance traveled by a small molecule like a tracking dye. (wikipedia.org)
  • In most proteins, the binding of SDS to the polypeptide chains impart an even distribution of charge per unit mass, thereby resulting in a fractionation by approximate size during electrophoresis. (wikipedia.org)
  • Proteins were resolved using a 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). (cdc.gov)
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
  • Gel drying equipment is used to dry the agarose or polyacrylamide gel medium used in gel electrophoresis to separate macromolecules DNA, RNA and proteins. (news-medical.net)
  • Sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis of [3H]LSD labeled membranes from F. hepatica showed two proteins which were selectively labeled by the photoreactive [3H]LSD. (erowid.org)
  • This method of direct photolabeling with non-derivatized [3H]LSD may allow identification of LSD-binding proteins in a variety of systems. (erowid.org)
  • Purpose: Saliva contains both specific and non-specific protective factors of the immune system, such as antimicrobial proteins, which can inhibit the adhesion and viability of cariogenic microorganisms. (pucrs.br)
  • To examine gender-dimorphic regulation of skeletal muscle proteins between healthy control and STZ-induced diabetic rats of both genders, we performed differential proteome analysis using two-dimensional electrophoresis combined with mass spectrometry. (karger.com)
  • The proteins resolved by IEF on IPG strips in the first dimension are applied to an SDS polyacrylamide gel and separated by size (molecular weight) in a direction perpendicular to the first dimension. (bio-rad.com)
  • First-Dimension Separation (Isoelectric Focusing) , Protein Sample Preparation for 2-D Electrophoresis , Staining and Visualization of Proteins After 2-D Electrophoresis , Imaging and Analysis of 2-D Electrophoresis Gels , Protein Spot Excision and Protein Identification , and Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor™ . (bio-rad.com)
  • The pores of the second-dimension gel sieve proteins according to size because SDS coats all proteins with a negative charge essentially in proportion to their mass. (bio-rad.com)
  • Homogeneous (single-percentage acrylamide) gels generally give excellent resolution of sample proteins that fall within a narrow MW range. (bio-rad.com)
  • Gradient gels have 2 advantages: they allow proteins with a wide range of MW to be analyzed simultaneously, and the decreasing pore size along the gradient functions to sharpen the spots. (bio-rad.com)
  • Metyrapone of proteins/street was diluted in regular SDS test buffer and put through electrophoresis on 12% SDS\polyacrylamide gels. (holyexperiment.org)
  • The proteins were expressed and secreted by using a baculovirus expression system and have been shown to bind to monoclonal antibodies which recognize discontinuous epitopes and to complement component C3b in a dose-dependent manner. (nih.gov)
  • Orthopoxviruses can evade host immune responses by secreting proteins that antagonize the functions of host IFNγ, CC and CXC chemokines, IL-1ß, and the complement system. (bvsalud.org)
  • This microbiota is influenced by both specific (antibodies) and non-specific (innate) components such as antimicrobial proteins lysozyme (LZ) and lactoferrin (LF) associated with the secretory immunologic system. (bvsalud.org)
  • In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)
Agarose gel electrophor2
  • Cartilage extracted glycosaminoglycans (GAGs) concentration was assessed after agarose gel electrophoresis. (usp.br)
  • Gastroenteritis was considered HA if symptoms primers, agarose gel electrophoresis, and ethidium bromide developed 48 hours after admission. (cdc.gov)
Nucleic Acid Electrophoresis2
Precast gels5
  • ready-to-use precast gels can be purchased, or you can hand cast polyacrylamide gels on the bench. (bio-rad.com)
  • Precast gels ensure high reproducibility among samples and help reduce the work involved in running large numbers of samples. (bio-rad.com)
  • Bio-Rad offers several lines of electrophoresis cells and precast gels for 2-D experiments. (bio-rad.com)
  • Both handcast and Mini-PROTEAN TGX Precast Gels can be used with these cells. (bio-rad.com)
  • Criterion precast gels provide increased resolution over a mini gel system but still run fast enough to let you generate 2-D data from your sample in a single day. (bio-rad.com)
Protein5
  • A total of 1 mL of saliva was collected for protein analysis using a polyacrylamide gel electrophoresis (SDS-PAGE). (pucrs.br)
  • No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. (scielo.br)
  • Protein bands were visualized using an Odyssey Infrared Imaging System (Li\Cor Biosciences, USA). (holyexperiment.org)
  • The molecular weight and extent of glycosylation of gC1 (457t), gC1(delta33-123t), and gC2(426t) were determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometric analysis. (nih.gov)
  • Protein and Protein-Amphiphile Systems. (lu.se)
Sodium2
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight. (wikipedia.org)
  • Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) has been employed to analyze the constituents of soluble extracts obtained from intact fungal cells and those of isolated cell walls. (scialert.net)
Eletroforese em gel de poliacrilamida2
  • O tamanho molecular dos GAGs foi medido após eletroforese em gel de poliacrilamida. (usp.br)
  • Foi realizado exame clínico, para diagnosticar a presença ou ausência de cárie através do Índice CPO-D e coletado 1 mL de saliva para análise das proteínas por meio da eletroforese em gel de poliacrilamida (SDS-PAGE). (bvsalud.org)
Molecular weight1
Primers2
  • Real-time PCR (Applied Biosystems 7500 RT-PCR System) using matrix gene specific forward and reverse primers, used a standard curve method for copy number calculations. (cdc.gov)
  • Recently, metagenomics a new approach to identify culturable and non culturable bacteria with the help of 16S rDNA sequences isolate total DNA from HCH contaminated soil and amplified 16S rDNA through universal primers and confirmed its total population with denaturing gradient gel electrophoresis and sequencing. (ispub.com)
Gradient gel2
Acrylamide3
  • Acrylamide is toxic to the human nervous system, therefore all safety measures must be followed when working with it. (wikipedia.org)
  • Acrylamide is soluble in water and upon addition of free-radical initiators it polymerizes resulting in formation of polyacrylamide. (wikipedia.org)
  • It is useful to make polyacrylamide gel via acrylamide hydration because pore size can be regulated. (wikipedia.org)
Membranes1
  • The formation of lipid rafts and cavioli in biological membranes provides examples of cooperative association of lipids presumably assisted by the presence of cholesterol acting as a lineactant in the two-dimensional system of the membrane. (lu.se)
Composition1
Analyze1
Buffer1
Native2
  • As with all forms of gel electrophoresis, molecules may be run in their native state, preserving the molecules' higher-order structure. (wikipedia.org)
  • Native-PAGE keeps the oligomeric form intact and will show a band on the gel that is representative of the level of activity. (wikipedia.org)
Detection2
  • All specimens were tested using the Synthetic Peptide Enzyme Immunoassay (EIA) (Genetic Systems HIV-1/HIV-2 Peptide EIA) for the detection of antibody to human immunodeficiency virus type 1 or type 2 (HIV-1 and HIV-2) or both (Bio-Rad Laboratories, Hercules, CA). Any specimen that reacted in an initial test was retested in duplicate with the Genetic Systems HIV-1/HIV-2 Peptide EIA. (cdc.gov)
  • All specimens were tested using the Synthetic Peptide Enzyme Immunoassay (EIA) (Genetic Systems HIV-1/HIV-2 Peptide EIA) for the detection of antibody to human immunodeficiency virus type 1 or type 2 (HIV-1 or HIV-2) or both (Bio-Rad Laboratories, Redmond, WA). (cdc.gov)
Membrane1
  • It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)
Horizontal1
PAGE1
Immune4
  • Toll-like receptors (TLRs) are the major pattern recognition receptors of the innate immune system that sense a wide range of "danger" signals or pathogen-associated molecular patterns (PAMPs) ( 6 - 8 ). (frontiersin.org)
  • Their critical role requires them to maintain physiological traffic and enable homing of cells that largely belong to a specific immune system. (researchsquare.com)
  • suggested that the interaction between the intestinal environment, the barrier function and the immune system are crucial in the onset of type 1 diabetes [ 4 ]. (biomedcentral.com)
  • Recent studies have demonstrated that commensal bacteria are crucial for maturation and function of the mucosal immune system. (biomedcentral.com)
Strips1
Pores1
  • Polyacrylamide gel with small pores helps to examine smaller molecules better since the small molecules can enter the pores and travel through the gel while large molecules get trapped at the pore openings. (wikipedia.org)
Method2
  • Isotachophoresis is a method of electrophoresis that employs the basic principles of the stacking gel phase of multiphasic systems discussed in the preceding section. (nationaldiagnostics.com)
  • The CD4 cell counts were obtained by using the Becton Dickinson MultiTEST reagent in TrueCOUNT tubes (Becton Dickinson Immunocytometry Systems, San Jose, CA). Comparison of this method with CD4 counts calculated from CBC absolute lymph counts from fresh whole blood X CD4% from the frozen whole blood resulted in a correlation coefficient of 0.9872. (cdc.gov)
Cells2
  • In the case of solid tissues or cells, these are often first broken down mechanically using a blender (for larger sample volumes), using a homogenizer (smaller volumes), by sonicator or by using cycling of high pressure, and a combination of biochemical and mechanical techniques - including various types of filtration and centrifugation - may be used to separate different cell compartments and organelles prior to electrophoresis. (wikipedia.org)
  • This system can be readily isolated from Triturus red blood cells, and therefore lends itself very readily to ultrastructural and biochemical analyses. (torvergata.it)
Biological systems1
  • It is clear that colloid science has reached such a level that it can contribute with much larger consequences to the understanding of a range of processes in biological systems, than was possible during the fi rst half of the previous century. (lu.se)
Dryers1
  • Drying systems may include oven-like air drying racks or suction-based vacuum dryers. (news-medical.net)
Products2
Product line1
  • The Mini-PROTEAN Product Line includes the Mini-PROTEAN Tetra cell , which can hold up to four gels, and the high-throughput Mini-PROTEAN 3 Dodeca Cell (for running up to 12 mini gels). (bio-rad.com)
Complex3
  • One can now describe and understand the properties of increasingly complex systems. (lu.se)
  • Systems that previously were considered too complex for a molecular characterization now successively become available to fundamental studies. (lu.se)
  • iii) Molecular transport in complex systems. (lu.se)
Real-time2
  • Second, we quantify the force-dependent real-time shuttling kinetics of the macrocycle between the two stations and calculate the energy landscape of the system under our experimental conditions. (nature.com)
  • Real-time PCR (Applied Biosystems 7500 RT-PCR System). (cdc.gov)
Equipment2
  • Once submitted, we will try and place you in contact with a suitable Gel Drying Equipment supplier within 48 hours. (news-medical.net)
  • Owl Separation Systems offers equipment, services and support necessary for optimum laboratory throughput & efficiency for gel electrophoresis. (biosciregister.com)
Includes1
  • Bio-Rad's Criterion™ midi gel system includes the Criterion™ cell (for 1-2 gels) and Criterion™ Dodeca™ cell (for 1-12 gels). (bio-rad.com)
Band1
  • The 90K glycoprotein is presumed to be related to the unusual stability of the microtubular system forming the marginal band. (torvergata.it)
Articles1
  • Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal. (ijpsonline.com)