AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Chromatography, GelChromatography, High Pressure LiquidMolecular WeightLanolinChromatography, Ion ExchangeElectrophoresis, Polyacrylamide GelChromatography, AffinityChromatographyGelsChromatography, LiquidMolecular Sequence DataKineticsAmino Acid SequencePolyestersCarbohydrate SequenceHydrogen-Ion ConcentrationAmino AcidsSubstrate SpecificityPolymersHydrolysisMagnetic Resonance SpectroscopyOligosaccharidesSolubilityCattleTemperaturePolyethylene GlycolsPolysaccharidesChromatography, GasEscherichia coliMacromolecular SubstancesDrug CarriersCircular DichroismRecombinant ProteinsPolysaccharides, BacterialChromatography, Thin LayerHot TemperaturePeptide FragmentsProtein ConformationDrug StabilityProtein BindingMass SpectrometryCloning, MolecularBacterial ProteinsMicroscopy, ElectronLiverSkin AbsorptionStructure-Activity RelationshipChromatography, DEAE-CellulosePermeabilityChemical FractionationTandem Mass SpectrometryChromatography, Reverse-PhaseGas Chromatography-Mass SpectrometryElectrophoresis, Gel, Two-DimensionalSpectrophotometry, UltravioletSpectrometry, Mass, Electrospray IonizationChemistryChemical PhenomenaIonic LiquidsChromatography, AgaroseCarbohydratesTrypsinRadioimmunoassayPeptidesSwineCarbohydrate ConformationAdministration, CutaneousIsoelectric PointMolecular StructureUltracentrifugationCell Membrane PermeabilityIsoelectric FocusingReproducibility of ResultsSpecies SpecificityGlycoside HydrolasesReference StandardsIndicators and ReagentsRats, Inbred StrainsCalibrationRabbitsElectrophoresis, Gel, Pulsed-FieldChromatography, PaperGlucansGlycoproteinsElectrophoresis, Agar GelSepharoseLiquid CrystalsBase SequenceRats, HairlessEnzyme StabilityCarrier ProteinsWaterCells, CulturedAcetonitrilesLimit of DetectionMethylationSolid Phase ExtractionChemistry, PharmaceuticalSolventsMicrochemistry
HPLCMass SpectrometryStationary phaseThin layer chromatoSilicaSupercritical fluid chromatoSeparationChromatographic techniquesPurificationExclusion chromatographyPolymersFlash chromatographyExchange chromatographySpectroscopyDiode arrayDetectionFiltrationThermogravimetricCompoundsAgilentSolventEluentCharacterizationAgaroseAnalyticalInorganicIonicMixturesPreparativeBeadsSyntheticMolecular weightsAnalysisLaboratoriesSamplesSizeUltravioletTECHNIQUEColumnPrincipleFragmentsHighBulkTestAdsorptionSystemChemicalsCarbon dioxideParticles
HPLC7
  • In most laboratories, chromatography is performed using high-performance liquid chromatography (HPLC) or gas chromatography (GC) . (coleparmer.com)
  • In HPLC, liquid serves as the mobile phase, and the stationary phase is most often found inside a column (a polymer or stainless-steel tube filled with small spherical particles that have specially modified surfaces to selectively interact with molecules in different ways). (coleparmer.com)
  • Gas chromatography (GC, GC-MS etc.) is typically applicable to volatile and semi-volatile, thermally stable compounds, whereas liquid chromatography (LC-MS, HPLC etc.) complements GC to provide analysis for medium to low volatility, thermally labile and highly polar compounds. (smithersapex.com)
  • A minimum of three years of experience in pharmaceutical analytical testing, including proficiency in conducting High-Performance Liquid Chromatography (HPLC), Gel Permeation Chromatography (GPC), and Dissolution analysis. (planet-pharma.com)
  • Article: Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD), Katharina Anne Scherf, Herbert Wieser, and Peter Koehler, Journal of Agricultural and Food Chemistry , doi: 10.1021/acs.jafc.6b02512, published online 16 September 2016. (healthnewsground.com)
  • Evolving to become more recently known as high performance liquid chromatography, HPLC systems work faster and at higher pressures than some other types of liquid chromatography systems, in part because the mobile phase can be introduced under greater pressure than with other techniques. (teledyneisco.com)
  • HPLC can also be used for the preparative purification of compounds, resulting in preparative high performance liquid chromatography systems like the ACCQPrep HP150. (teledyneisco.com)
Mass Spectrometry4
Stationary phase13
  • The principle of separation relies on the differential exclusion or inclusion of the macro molecules by the porous gel stationary phase. (wikipedia.org)
  • At its core, chromatography is the interaction between a mobile phase (which carries the mixture being separated) and a stationary phase (which performs the separation). (coleparmer.com)
  • Most chromatography methods have an inert mobile phase that carries the analyte through a long stationary phase housed inside a column. (coleparmer.com)
  • For example, in gel permeation chromatography, special inert beads are used as the stationary phase, and the mobile phase carries the analyte past those beads. (coleparmer.com)
  • A form of gas chromatography in which the moving phase is a gas and the stationary phase is a surface-active sorbent (charcoal, silica gel, or activated alumina). (termwiki.com)
  • In size exclusion chromatography, the stationary phase is. (web.app)
  • Bare silica gel is an acceptable stationary phase. (tsijournals.com)
  • The chemical attachment of suitable groups, usually C18 alkyl groups, onto a carrier, primarily silica gel, produces the practically applicable volume of LC stationary phase. (tsijournals.com)
  • In high performance liquid chromatography, the mobile phase is pushed through the stationary phase under higher pressure than that used for flash chromatography. (teledyneisco.com)
  • The higher pressure is a result of using a stationary phase with smaller particle size than that used for flash chromatography resulting in increased resolution. (teledyneisco.com)
  • Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
  • A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
  • It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
Thin layer chromato1
Silica4
  • The samples were analysed using pressurized liquid extraction, silica multilayer column followed by gel permeation chromatography for purification and GC-EI-HRMS for separation and detection. (inrae.fr)
  • At 35 °C, two separate liquid chromatographic critical ranges were discovered using non-end-capped silica gel C-18 column packing, poly (methyl methacrylate)s (PMMAs), and mixed eluents including acetonitrile (ACN) and toluene. (tsijournals.com)
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
  • Such a solution could be used in a passive monitor or coated onto silica gel. (cdc.gov)
Supercritical fluid chromato1
Separation8
  • Scholars@Duke publication: Separation of limited tryptic fragments of human ceruloplasmin by gel-permeation high-performance liquid chromatography. (duke.edu)
  • In order to locate the sites of proteolytic cleavage in the whole molecule, we used gel-permeation high-performance liquid chromatography to determine the optimum conditions for fragment separation. (duke.edu)
  • Today, most applications of chromatography are generally less colorful but the underlying principle of separation remains unchanged. (coleparmer.com)
  • Gel permeation chromatography gpc is a chromatographic technique in which the separation obtained is a function of molecular size. (web.app)
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
  • Gel permeation chromatography gpc is a column separation technique based on a nonionic molecularsieve effect and, in contrast to other chromatographic. (web.app)
  • Chromatography provides separation of the substances in your sample, followed by quantification and/or identification of the individual substances. (smithersapex.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
Chromatographic techniques4
  • Evaluation of gel permeation chromatographic techniques. (web.app)
  • Chromatographic techniques have applications in all industry sectors - whether you need regulatory or legislation-driven testing, assistance with production issues, failure diagnosis or data to support research and development, chromatography can help to provide a solution. (smithersapex.com)
  • Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (lookformedical.com)
  • Chromatographic techniques in which the mobile phase is a liquid. (lookformedical.com)
Purification2
  • Chromatography can be used in a myriad of applications from analyzing miniscule samples to production-scale use as a purification step. (coleparmer.com)
  • POMC IV was subjected to further purification on a Sephadex G-50 gel filtration column. (indexindex.com)
Exclusion chromatography10
Polymers6
  • The resulting polymers (RPESO) were characterized using Infrared (IR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), 1H NMR, 13C NMR, solid state 13C NMR, and gel permeation chromatography (GPC). (usda.gov)
  • Gel Permeation Chromatography (GPC), abbreviated as GPC, utilizes the differential elution time of molecules of various sizes through a gel column to separate molecules of different molecular weights in high polymers. (cirs-ck.com)
  • Gel permeation chromatography on branched polymers. (web.app)
  • Moore, of the dow chemical company, published his work on the preparation of gel permeation chromatography gpc and changed how scientists studied synthetic polymers and. (web.app)
  • Synthetic polymers are subjected to two-dimensional liquid chromatography, or 2D-LC. (tsijournals.com)
  • In this approach, the existence of surface adsorption and enthalpic partition as two similar but distinct enthalpic retention mechanisms in high-performance liquid chromatography of polymers was indirectly demonstrated. (tsijournals.com)
Flash chromatography1
Exchange chromatography1
Spectroscopy3
  • We provide the scientific community with state-of-the-art microanalytic capabilities, including microscopy, chromatography, spectroscopy and structural analysis. (xerox.com)
  • The monomers were analyzed qualitatively and quantitatively by high performance liquid chromatography, nuclear magnetic resonance spectroscopy, and gel permeation chromatography. (niom.no)
  • Firstly, a triblock thermosensitive copolymer PLGA-PEG-PLGA that consists of poly(D,L-lactic acid-co-glycolic acid)-b-poly(ethylene glycol)-b-poly(D,L-lactic acid-co-glycolic acid) was synthesized via living ring-opening polymerization under an inert atmosphere and characterized via proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. (nusl.cz)
Diode array1
  • High-performance liquid chromatography with diode array detection system was used for identification and quantification of the monomers in the resin systems. (niom.no)
Detection1
  • The researchers combined gel-permeation high-performance liquid chromatography with fluorescence detection to develop a sensitive technique that can detect both gliadins and glutenins in purified wheat starch. (healthnewsground.com)
Filtration1
  • Difference between gel filtration and gel permeation. (web.app)
Thermogravimetric1
  • Purity of extracted agarose was confirmed from various spectral and analytical techniques, such as 1 H and 13 C NMR, FTIR, circular dichroism (CD), gel permeation chromatography (GPC) and thermogravimetric analysis (TGA). (scirp.org)
Compounds2
  • A variety of chromatography equipment is also available that can provide an accurate identification of known compounds or in-depth data about unknown compounds. (coleparmer.com)
  • The similarities and differences of two-dimensional liquid chromatography of low-molecular-mass and polymeric compounds are discussed. (tsijournals.com)
Agilent1
Solvent1
  • Analytes that are totally excluded, elute with the free volume outside around the particles (Vo), the total exclusion limit, while analytes that are completely delayed, elute with the solvent, marking the total permeation volume of the column, including also the solvent held inside the pores (Vi). (wikipedia.org)
Eluent1
  • Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N(C 2 H 5 ) 3 -H 2 CO 3 buffer as the eluent. (mssm.edu)
Characterization1
  • Characterization of an oligomeric compound was performed using gel permeation liquid chromatography. (niom.no)
Agarose2
  • We explored the possibility of using ionic liquids (ILs) as medium for efficient extraction of agarose via dissolution of red algae under varying conditions of heating or microwave irradiation. (scirp.org)
  • The physicochemical properties, such as gelling or melting temperature, viscosity and gel strength of extracted agarose hydrogels have been measured and compared with the agarose obtained from similar source reported in the literature. (scirp.org)
Analytical3
  • Instrumentation available waters gpc viscotek gpc max gel permeation chromatography gpc from eag website gel permeation chromatography gpc is an analytical technique for the. (web.app)
  • The development of an analytical procedure for measuring free cyanide , the various solid and liquid sorbents tested, and the instability problems encountered during research to establish a monitoring method for hydrogen - cyanide (74908) (HCN) in air are described. (cdc.gov)
  • The original goals of the project were the development of improved sampling and analytical methods for hydrogen -fluoride (7664393), HCN, and particulate cyanides by replacing liquid impinger samplers with solid sorbent samplers and using an ion chromatographic analytical technique. (cdc.gov)
Inorganic1
  • To separate and analyse complex mixtures or solutions which include liquids and solids of both organic and inorganic origins. (sctimst.ac.in)
Ionic2
  • Alcohols of increasing alkyl chain length were investigated as precipitants in an ionic liquid (IL) pretreatment system. (metu.edu.tr)
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
Mixtures1
  • Chromatography is a technique for separating mixtures into their constituent parts. (coleparmer.com)
Preparative1
Beads1
  • Composed of semipermeable, porous polymer gel beads with well defined range of pore sizes. (web.app)
Synthetic1
Molecular weights1
  • Shimpack gpc 80m mixed gel columns 80mc, 80md mixed gel columns that produce linear calibration curves over a wide range of molecular weights. (web.app)
Analysis3
  • Analysis on the market size, market leaders, and factors affecting the market of chromatography reagents from the perspective of its end users, which includes the pharma and biotech, food and beverages, environmental agencies and other sectors. (bccresearch.com)
  • Request pdf analysis of chitosan by gel permeation chromatography the effect of. (web.app)
  • The technique of gel permeation chromatography gpc was utilized in analysis of paraffinic wax samples generated via the fischertropsch synthesis in a slurry reactor. (web.app)
Laboratories1
Samples1
  • For powder samples, a minimum of 50g is required, while for liquid samples, it should be between 50-100ml. (cirs-ck.com)
Size1
  • GPC is a type of chromatography in which analytes are separated, based on their size or hydrodynamic volume (radius of gyration). (wikipedia.org)
Ultraviolet1
  • The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns and ultraviolet (UV) absorption spectrums of ASC-M and PSC-M illustrated that they were mainly composed of type I collagen. (bvsalud.org)
TECHNIQUE2
Column2
Principle1
Fragments1
High5
Bulk1
  • Only at distances in excess of this would the film acquire the completely liquid-like properties which would enable its angle of contact with the bulk liquid to become zero thinner films would differ in structure from the bulk liquid and should therefore display a finite angle of contact with it. (chempedia.info)
Test1
Adsorption1
  • In general there are two factors capable of bringing about the reduction in chemical potential of the adsorbate, which is responsible for capillary condensation the proximity of the solid surface on the one hand (adsorption effect) and the curvature of the liquid meniscus on the other ( Kelvin effect ). (chempedia.info)
System2
  • Tender fee emd 1 gpc gel permeation chromatography system 01 rs. (web.app)
  • Using nanoliter volumes this liquid handling system pipets your optimisation screen directly into the crystallisation plate (pipetting range: 200 nL - 4 mL). (lu.se)
Chemicals1
Carbon dioxide1
  • Ring opening polymerization of epoxidized soybean oil (ESO) initiated by boron trifluoride diethyl etherate was conducted in liquid carbon dioxide. (usda.gov)
Particles1
  • The smaller analytes relative to the pore sizes can permeate these pores and spend more time inside the gel particles, increasing their retention time. (wikipedia.org)