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Chromatography, GelGelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, Ion ExchangeChromatographyMolecular WeightChromatography, GasElectrophoresis, Polyacrylamide GelChromatography, Thin LayerChromatography, AgaroseChromatography, DEAE-CelluloseAmino AcidsKineticsChromatography, LiquidFiltrationIsoelectric FocusingMolecular Sequence DataHydrogen-Ion ConcentrationAmino Acid SequenceCarbohydratesChemistryChemical PhenomenaSubstrate SpecificityUronic AcidsChondroitinGlomerular Filtration RateUltracentrifugationCattleElectrophoresis, Gel, Two-DimensionalRed-Cell Aplasia, PureSepharoseElectrophoresis, Agar GelElectrophoresisChemical FractionationIsoelectric PointImmunodiffusionGlycosaminoglycansMass SpectrometryRabbitsImmunoelectrophoresisCentrifugation, Density GradientProtein BindingProteoglycansRadioimmunoassayPeptide FragmentsMacroglobulinsEscherichia coliMethodsOligosaccharidesElectrophoresis, Gel, Pulsed-FieldPhysicochemical PhenomenaChemistry, PhysicalSpectrophotometry, UltravioletLiverTrypsinTemperatureSolubilityCarbohydrate SequenceSulfatesMagnetic Resonance SpectroscopyBinding SitesPeptidesGlycoproteinsSwineChondroitin SulfatesImmunoelectrophoresis, Two-DimensionalProtease InhibitorsDermatan SulfateLaryngeal CartilagesGas Chromatography-Mass SpectrometryProteinsPeptide HydrolasesChromatography, PaperPolysaccharidesBase SequenceProtein ConformationSulfur IsotopesSodium Dodecyl SulfateSpectrophotometrySpecies SpecificityHyaluronoglucosaminidaseRats, Inbred StrainsChromatography, Reverse-PhaseSulfuric AcidsMicroscopy, ElectronEndopeptidasesCarrier ProteinsEdetic AcidStreptomycesCarbohydrate ConformationHydroxyapatitesDrug StabilityPhysiciansCircular DichroismRecombinant ProteinsChemical PrecipitationCell LineImmune SeraHydrolysis
AffinityPurificationElectrophoresisPermeation chromatographyCation exchangeResinsColumnsSepharoseProteinsAssaysSilicaIsolationMethodsPolysaccharideMolecularSeparatePartiallySizeOrganicFractionHighAnalysisApplicationsActivityLiquid ChromatographyMass spectrometryAmmonium sulfate preciProteinPartiallyChromatographicUltracentrifugationFractionationFractionsBufferSolubleDetectionSeparationSubsequentSamplesSizeColumnTechniques
Affinity5
Purification4
  • Explore a complete line of chromatography resins and media with various base bead and ligand chemistries for your lab-scale and bioproduction purification needs. (bio-rad.com)
  • From high-throughput resin screening to small-scale method development and scale-up optimization, find chromatography resins in prepacked formats for the entire purification development cycle. (bio-rad.com)
  • Open in another window Shape 2 (A) Gel purification chromatography (FPLC) of the quickly eluting LC. (mingsheng88.org)
  • A nearly 136-fold enzyme purification was obtained from chicken erythrocyte by acetone precipitation, ethanol-chloroform treatment, CM-cellulose and Sephadex G-200 chromatography. (tubitak.gov.tr)
Electrophoresis3
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
  • SDS-gel electrophoresis results indicated that chicken erythrocyte catalase consists of four apparently identical subunits, with a molecular weight of around 57.5 kDa. (tubitak.gov.tr)
  • Two representative urine samples containing lambda or kappa FLC were loaded into a nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). (bvsalud.org)
Permeation chromatography1
Cation exchange1
  • Purify charged and polar molecules with anion and cation exchange chromatography (AEX, CEX) resins available in various particle sizes, ionic forms, and binding capacities. (bio-rad.com)
Resins3
  • Size exclusion chromatography applications for separating macromolecules based on subtle differences in size typically use resins with large and varied pore sizes in long chromatography columns. (wikipedia.org)
  • Experience unparalleled selectivity, resolution, yield and ease-of-use for a variety of biomolecules with the unique separation properties of our multimodal chromatography resins. (bio-rad.com)
  • Explore a wide selection of size exclusion (or gel filtration) chromatography resins for diverse applications, including those requiring use of organic eluents. (bio-rad.com)
Columns6
  • COSMOSIL Diol-120-II, Diol-300-II and Diol-1000-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. (nacalaiusa.com)
  • There are a number of common formats for performing gel filtration for smaller (less than 4mL) volumes: Chromatography columns Gravity-flow columns Chromatography cartridges Centrifuge columns Centrifuge plates Gravity-flow, or drip, columns use head-pressure from a buffer-chase to push the sample through the gel filtration matrix. (wikipedia.org)
  • Sealed chromatography cartridges or columns work similarly except the sample and buffer is pumped into and through the resin by an external device such as a liquid chromatographic (LC) system, also requiring collection and monitoring of several fractions. (wikipedia.org)
  • Shim-pack MAqC-ODS I reversed-phase columns are packed with a silica gel containing metal and added octadecylsilyl group. (shimadzu.com)
  • Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. (shimadzu.com)
  • Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. (shimadzu.com)
Sepharose1
Proteins1
Assays1
  • The FLC were recovered from SDS-PAGE gel in sufficient amounts to be quantified by UV and two automated nephelometric assays immunochemical. (bvsalud.org)
Silica15
  • With the silica-based gel, organic solvents, including methanol and acetonitrile, can be use. (nacalaiusa.com)
  • Silica Gel Market Strategic Insights 2020 with Analysis of Leading Players: Evonik Industries, Clariant, Solvay, Merck Group, Etc. (sbwire.com)
  • Reports and Data's newest report titled 'Global Silica Gel Market Forecast to 2027' is inclusive of an all-encompassing study of the global Silica Gel market. (sbwire.com)
  • The report offers a panoramic view of the Silica Gel industry, and, at the same time, offers useful insights into the estimated Silica Gel market size, revenue share, and sales & distribution networks over the forecast years (2019-2027). (sbwire.com)
  • Venture capitalists are the primary addressees of this report, which gives them a vivid conception of the competitive spectrum of the global Silica Gel market. (sbwire.com)
  • In the further segment of the report, researchers have provided the latest coverage of the profound impact of the ongoing COVID-19 pandemic on the global Silica Gel market. (sbwire.com)
  • The coronavirus outbreak has led to drastic changes in the current Silica Gel business landscape, limiting the growth opportunities for various manufacturers and buyers in this industry for the next few years. (sbwire.com)
  • Moreover, the report offers an exhaustive Silica Gel market overview and elaborates on the financial standing of the leading players in the current market situation. (sbwire.com)
  • A broad Silica Gel market segmentation is the focal point of the report that includes the product type gamut, application spectrum, end-user industry landscape, significant geographical regions, and the top market contenders. (sbwire.com)
  • Our expert team has employed several analytical tools like investment assessment, SWOT analysis, and Porter's Five Forces Analysis to accurately evaluate the production capacity of the Silica Gel market. (sbwire.com)
  • The global Silica Gel market is categorized into several leading geographical regions, including North America, Europe, Latin America, Asia Pacific, and Middle East & Africa. (sbwire.com)
  • Under this section of the report, the global Silica Gel market presence across the major regions in terms of the global market share, market size, revenue contribution, sales network, and distribution channels has been analyzed. (sbwire.com)
  • What are the key factors driving the global Silica Gel market? (sbwire.com)
  • What are the market opportunities and risks affecting the performance of the vendors in the global Silica Gel market? (sbwire.com)
  • Over the course of many years, surface bonding chemistries have progressed to give today's chemists access to many bonded phase silica gel options. (sorbtech.com)
Isolation1
Methods3
Polysaccharide2
Molecular1
Separate1
  • The gel strips containing separate monomers and dimers were excised, electroeluted, and the FLC recovered. (bvsalud.org)
Partially1
Size2
Organic1
  • 1992. Determining volatile organic compounds in human blood from a large sample population by using purge and trap gas chromatography/mass spectrometry. (cdc.gov)
Fraction1
  • The macromolecular components are recovered in the buffer used to pre-equilibrate the gel-filtration matrix, while the small molecules can be collected in a later fraction volume or be left trapped in the resin. (wikipedia.org)
High1
Analysis1
  • A BLAST search of internal peptide sequences obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis suggested that the enzyme belonged to the GH27 family. (nih.gov)
Applications1
Activity1
Liquid Chromatography6
Mass spectrometry5
Ammonium sulfate preci1
Protein1
Partially2
Chromatographic2
  • Sealed chromatography cartridges or columns work similarly except the sample and buffer is pumped into and through the resin by an external device such as a liquid chromatographic (LC) system, also requiring collection and monitoring of several fractions. (wikipedia.org)
  • Beginning with harvest of material from a bioreactor, downstream processing removes or reduces contaminants to acceptable levels through several steps that typically include centrifugation, filtration, and/or chromatographic technologies. (bioprocessintl.com)
Ultracentrifugation2
Fractionation2
  • Gamma globulins are purified from non-immunized animal serums by salt fractionation, ion-exchange chromatography and gel filtration. (jacksonimmuno.com)
  • Fractionation of the digests by gel filtration chromatography disclosed that maximal chemotactic activity eluted in fractions corresponding to 14,000-20,000 mol wt containing most of the desmosine cross-links in the digests. (jci.org)
Fractions1
  • The fractions with the highest luminescence activity were loaded onto a column with Sephacryl S-300 in 50 mM Na-phosphate buffer (pH 7.0) containing 150 mM NaCl and 0.04% DDM for gel filtration. (springer.com)
Buffer6
  • Desalting and buffer exchange are two of the most common gel filtration chromatography applications, and they can be performed using the same resin. (wikipedia.org)
  • The macromolecular components are recovered in the buffer used to pre-equilibrate the gel-filtration matrix, while the small molecules can be collected in a later fraction volume or be left trapped in the resin. (wikipedia.org)
  • Dialysis is useful for many of the same desalting and buffer exchange applications performed with gel filtration chromatography, as both methods are based on similar molecular weight cut-off limits. (wikipedia.org)
  • There are a number of common formats for performing gel filtration for smaller (less than 4mL) volumes: Chromatography columns Gravity-flow columns Chromatography cartridges Centrifuge columns Centrifuge plates Gravity-flow, or drip, columns use head-pressure from a buffer-chase to push the sample through the gel filtration matrix. (wikipedia.org)
  • These may be supplied in a variety of different formats such as in solution in a neutral buffer, a soluble lyophilisate, and contained in gel slices or on nitrocellulose blots. (southernbiotech.com)
  • Molecules are separated by size differences as they pass through a resin packed in a column, and molecules do not bind to the chromatography resin, meaning buffer composition does not directly affect resolution, which is the degree of separation between peaks. (pharmaceuticalonline.com)
Soluble1
  • In normal phase chromatography, fat soluble substances move faster than water soluble ones, whereas in reverse phase the water soluble substances move fastest. (uu.se)
Detection1
  • For preparative extraction, we used DDM at a concentration of 10 mM, due to its availability and good applicability for chromatography with UV detection. (springer.com)
Separation3
  • This activity simulates the extraction, identification, and separation of chemicals in or on plants using chromatography. (nih.gov)
  • In general, the size exclusion chromatography is a method which concentrates on solvent separation, wherein molecules are separated as per their molecular size. (powershow.com)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (nih.gov)
Subsequent1
  • During the subsequent chromatography steps, stable p66/p51 heterodimer was purified to homogeneity. (nih.gov)
Samples2
  • Gel filtration has the advantage of speed (a few minutes vs. hours for dialysis) along with the ability to remove contaminants from relatively small-volume samples compared to dialysis which is an important feature when working with toxic or radioactive substances. (wikipedia.org)
  • 7. Make the standards: Label 4 pieces of chromatography paper with A, B, C, & D. Place samples with the primary colors (A= 3 = blue, B = 4 = yellow, C = 1 = red, D = 2 = green) on the bottom line. (nih.gov)
Size5
Column1
  • one column of anion exchanger, one column of gel filtration, and again one column of anion exchanger. (nih.gov)
Techniques1