AffinityPurificationHPLCAnalyticalDialysisPeptideFPLCMass spectrometryProteinProteinsResinsMolecular weight markerSepharoseActivated aluminaHydrophilicFractionationIsolationChemically-bondedDetectorMarkerSphericalPorousMixtureDextranDiolCommonlyReproducibilityEffectivelyTemperatureHighSizeAnalysisRangeDeterminationReversed-phaseOrganic
Affinity6
- Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. (wikipedia.org)
- Like general chromatography, but using dyes to apply on a support matrix of a column as the stationary phase that will allow a range of proteins with similar active sites to bind to, refers to as pseudo-affinity. (wikipedia.org)
- Based on the properties of the protein to be purified, appropriate methods are selected such as ion exchange chromatography, gel filtration chromatography, and affinity chromatography. (fujifilm.com)
- If the protein of interest has no ligand that can be used for affinity chromatography, an affinity tag (epitope tag) can be added to the protein and purified using the ligand for the affinity tag. (fujifilm.com)
- The purification steps included heating at 37° for 2 hr, deoxycholate extraction, DE-52 column chromatography, Sephadex G-200 gel filtration and affinity chromatography. (utmb.edu)
- It was overexpressed in Escherichia coli in a soluble form and purified to electrophoretic homogeneity by His-Trap affinity chromatography. (springeropen.com)
Purification4
- Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography. (lumiprobe.com)
- Column chromatography is often used for protein purification. (fujifilm.com)
- The purification procedures included crude extraction, DEAE-cellulose anion exchange column chromatography, and Sephacryl S-300 gel filtration column chromatography. (springeropen.com)
- The MCI Gel™ size exclusion columns and resins are based on hydrophilic polyhydroxymethacrylates suitable for the analysis and purification of water soluble polymers like oligosacharides, proteins, PEG etc. (biokal.com)
HPLC5
- It can carry out in a conventional way by using as a packed column, or in high-performance liquid chromatography (HPLC) column. (wikipedia.org)
- Shim-pack FC-ODS is an ideal column to shorten your analysis time using conventional HPLC. (shimadzu.com)
- Shim-pack GIS series columns are packed with high purity silica gel and are your ideal generic HPLC columns. (shimadzu.com)
- 7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assayed for ACE inhibition and, in vivo, for edema potentiation. (biomedcentral.com)
- VertiSep ™ Integral HPLC Columns. (vertichrom.com)
Analytical2
- The differences in the compositions and percentages of polysaccharides are attributed to temperature changes, column chromatography, and analytical methods [ 10 , 11 ]. (hindawi.com)
- as molecular mass standard in analytical gel filtration chromatography for Superdex ® 75 10/300 GL gel filtration column. (sigmaaldrich.com)
Dialysis1
- Chitinase produced by Streptomyces albus was purified consecutively with dialysis, gel filtration and ion- exchange chromatography, the test isolate can be exploited for large scale production of chitinase. (springeropen.com)
Peptide2
- A BLAST search of internal peptide sequences obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis suggested that the enzyme belonged to the GH27 family. (nih.gov)
- The peptide fragments are then recovered from the gel and subjected to mass spectrometry (LC-MS/MS or MALDI-TOF MS) to determine the mass of each fragment. (fujifilm.com)
FPLC1
- In fast-protein liquid chromatography (FPLC) using Blue MX-R immobilized on poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads, it was seen to separate lysozyme and bovine serum albumin (BSA), purified lysozyme from chicken albumin. (wikipedia.org)
Mass spectrometry1
- Ethion can be recovered from saliva, urine, and plasma by extraction and analysis by gas chromatography with flame photometric detection (GC/FPD), or GC in conjunction with mass spectrometry (GC/MS) (Nigg et al. (cdc.gov)
Protein2
- A piece of gel containing the target protein is excised, and the protein is digested in the gel by proteases (peptidases). (fujifilm.com)
- The fibrin gel obtained from the venom of the Crotalus durissus terrificus snake, a rattlesnake, contains serinoprotease, a protein in the venom that has been characterized as thrombin-like and acts in a similar manner as thrombin. (scite.ai)
Proteins3
- Size Exclusion Chromatography or gel filtration is a widely used technique for separation of proteins and other molecules based on their sizes. (gbiosciences.com)
- COSMOSIL Diol-120-II, Diol-300-II and Diol-1000-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. (nacalaiusa.com)
- In SDS-PAGE, proteins are given negative charges by addition of sodium dodecyl sulfate (SDS) and then electrophoresed on a polyacrylamide gel. (fujifilm.com)
Resins1
- On this page: List of available columns and resins, followed by some sample chromatograms. (biokal.com)
Molecular weight marker1
- It can be used as a gel filtration molecular weight marker. (sigmaaldrich.com)
Sepharose2
- C29H20ClN7O11S3 + C24H38O19 → C53H57N7O30S3 + HCl Cibacron Blue FG3-A + Sepharose → Blue Sepharose + HCl The dyes used in this type of chromatography are inexpensive and generally available as they are from textile industries called reactive dye. (wikipedia.org)
- Usually available commercially with sepharose as the supporting matrix in the form of packed columns. (wikipedia.org)
Activated alumina1
- A form of gas chromatography in which the moving phase is a gas and the stationary phase is a surface-active sorbent (charcoal, silica gel, or activated alumina). (termwiki.com)
Hydrophilic2
- Shim-pack WAX/WCX series columns are chemically-bonded hydrophilic silica gel based ion exchange columns. (shimadzu.com)
- Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. (shimadzu.com)
Fractionation1
- Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. (sigmaaldrich.com)
Isolation1
- The isolation protocol involved ion-exchange chromatography and gel filtration on a Superdex75 column. (nih.gov)
Chemically-bonded1
- Shim-pack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. (shopshimadzu.com)
Detector1
- The principle of the method specified in ISO 8128-1:1993 is extraction of patulin from a test portion using ethyl acetate followed by partitioning of the extract with aqueous sodium carbonate solution, qualitative and quantitative determination of the patulin content by means of high-performance liquid chromatography using an ultraviolet detector. (ansi.org)
Marker1
- The blue dye is used as a void volume (V0) marker for a gel filtration column. (wikipedia.org)
Spherical1
- Shim-pack GIST series columns are packed with newly developed high-purity porous spherical silica. (shimadzu.com)
Porous1
- The best option here is B because it is used to separate size using porous bead packed in a column. (indiabix.com)
Mixture2
- It is type of high purity Silica gel in fine powder form which is use during separation of mixture of components. (silicagelsuppliers.com)
- The method specified in ISO 8128-2:1993 includes the extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column, and qualitative and quantitative determination by means of two-directional thin-layer chromatography. (ansi.org)
Dextran1
- G-Sep™ Size Exclusion Columns are composed of high performance rigid resin made up of co-polymer of agarose and dextran. (gbiosciences.com)
Diol3
- Shim-pack Diol series is a kind of gel filtration chromatography (GFC) column. (shimadzu.com)
- Description: * Installed between the liquid pump and the sample injector to protect the Shim-pack Diol column. (shopshimadzu.com)
- COSMOSIL Diol-II series shows excellent separation compared to competitors' columns. (nacalaiusa.com)
Commonly2
- Commonly used reactive dyes for chromatography can be separated according to their color index name or functional group. (wikipedia.org)
- Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. (scite.ai)
Reproducibility1
- Shim-pack VP series ensures high column-to-column performance reproducibility, which is ideal for method development and validation. (shimadzu.com)
Effectively1
- The dyes are immobilized on the column matrix effectively, since usually the dyes link to a monochlorotriazine or dichlorotriazine ring (triazine dye). (wikipedia.org)
Temperature1
- Use caps for room-temperature reactions and to easily transport columns. (coleparmer.com)
High3
- Shim-pack GWS series is an economical generic C18 column designed with high cost performance. (shimadzu.com)
- In vitro stability in human serum at 37°C was analyzed using radio-thin layer chromatography and radio-high-performance liquid chromatography. (snmjournals.org)
- ISO 8128-1:1993-Apple Juice, Apple Juice Concentrates And Drinks Containing Apple Juice - Determination Of Patulin Content - Part 1: Method Using High-Performance Liquid Chromatography is available on the ANSI Webstore. (ansi.org)
Size4
- Shim-pack XR Series columns use a 2.2 μm packing particle size and offer a skillful balance between resolution efficiency and pressure. (shimadzu.com)
- However Chromatography silica gel available in various mesh size powder range from 30 mesh to 400 mesh powder. (silicagelsuppliers.com)
- The columns are 7.5 mm x 600 mm (ID x L) packed with 10 µm particle size resin. (biokal.com)
- Gel filtration chromatography is also known as size exclusion chromatography. (indiabix.com)
Analysis2
- Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to C18 columns and is suitable for the analysis of halogenated compounds, positional isomers and charged bases. (shimadzu.com)
- Designed to maximize performance of LC systems, Shimadzu's Shim-pack Velox columns with core shell technology enable you to achieve increased separations and faster analysis times on any LC platform. (shimadzu.com)
Range3
- Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter LC columns, which are the next generation organic silica hybrid based columns. (shimadzu.com)
- Finally With continuous focus and efforts of our R&D Department, We are Introducing our new range of product in chromatography process. (silicagelsuppliers.com)
- Total volumes for the columns range from 1 to 150 mL. (coleparmer.com)
Determination1
- Description: Shim-pack GPC series columns are used for the determination of tetrahydrofuran (800 Series), chloroform (800C Series), and dimethylformamide (800D series). (shopshimadzu.com)
Reversed-phase1
- Shim-pack MAqC-ODS I reversed-phase columns are packed with a silica gel containing metal and added octadecylsilyl group. (shimadzu.com)
Organic1
- With the silica-based gel, organic solvents, including methanol and acetonitrile, can be use. (nacalaiusa.com)