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  • agarose
  • Agarose is available as a white powder which dissolves in near-boiling water, and forms a gel when it cools. (wikipedia.org)
  • Agarose exhibits the phenomenon of thermal hysteresis in its liquid-to-gel transition, i.e. it gels and melts at different temperatures. (wikipedia.org)
  • The gelling and melting temperatures vary depending on the type of agarose. (wikipedia.org)
  • The gelling and melting temperatures are therefore given at a specified agarose concentration. (wikipedia.org)
  • Natural agarose contains uncharged methyl groups and the extent of methylation is directly proportional to the gelling temperature. (wikipedia.org)
  • The agarose in the gel forms a meshwork that contains pores, and the size of the pores depends on the concentration of agarose added. (wikipedia.org)
  • On standing the agarose gels are prone to syneresis (extrusion of water through the gel surface), but the process is slow enough to not interfere with the use of the gel. (wikipedia.org)
  • The removal of agaropeptin in agarose substantially reduce the EEO, as well as reducing the non-specific adsorption of biomolecules to the gel matrix. (wikipedia.org)
  • The melting and gelling temperatures of agarose can be modified by chemical modifications, most commonly by hydroxyethylation, which reduces the number of intrastrand hydrogen bonds, resulting in lower melting and setting temperatures than standard agaroses. (wikipedia.org)
  • separation
  • These separation mechanisms operate essentially in superposition along the length of a gel filtration column to which an axial electric field gradient has been added. (wikipedia.org)
  • The advantages of this method include good separation of large molecules from the small molecules with a minimal volume of eluate, and that various solutions can be applied without interfering with the filtration process, all while preserving the biological activity of the particles to separate. (wikipedia.org)
  • With size exclusion chromatography, there are short and well-defined separation times and narrow bands, which lead to good sensitivity. (wikipedia.org)
  • concentrations
  • The initial fluid transformed into gel after a few months with concentrations of up to 1% clay by weight. (wikipedia.org)
  • Unlike the lower concentrations where the arrangement of clay particles was continually in flux, the particles above 1% concentration locked into a stable structure which is known as equilbrium gel. (wikipedia.org)
  • particles
  • Clay particles interact in an anisotropic way differing from the typical isotropic way of colloidal particles, which normally interact with all of their nearest neighbors when forming a gel. (wikipedia.org)
  • This lets clay particles connect in a chain and allows the gel to form at a low density. (wikipedia.org)
  • separate
  • Unlike other gels, it maintains the same consistency throughout its structure and is stable, which means it does not separate into sections of solid mass and those of more liquid mass. (wikipedia.org)
  • The difference with equilibrium gel is that it will not separate over time into two separate phase like all other gels. (wikipedia.org)
  • material
  • Finally, in contrast to dialysis, gel filtration chromatography allows the contaminating material to be removed in a relatively small volume (and left on the column), an important feature when working with toxic or radioactive substances. (thermofisher.com)
  • Equilibrium gel is similar to any gel in the way that it is a colloid in which the disperse phase has combined with the dispersion medium to produce a semisolid material. (wikipedia.org)
  • This is due to the fact that the gel would allow the coatings to be lighter, thus reducing the amount of material that enters the body. (wikipedia.org)
  • main
  • One of the main characteristics of Equilibrium gel as with soft matter is that it displays various mesocopic structures originating from a large number of internal degrees of freedoms of each molecule. (wikipedia.org)
  • high
  • The alpha-neurotoxin-like peptide can be separated from the protease inhibitor, Sephadex G-50 gel filtration chromatography can be used, in the presence of high salt (1M NaCl) and alkaline conditions (pH = 8.2). (wikipedia.org)
  • Producing a relatively high power for a given weight, the battery could be incorporated into microscope devices if the gel could be made at a low enough density. (wikipedia.org)
  • different
  • A variety of chemically modified agaroses with different melting and gelling temperatures are available through chemical modifications. (wikipedia.org)