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  • blot
  • In a dot blot the biomolecules to be detected are not first separated by electrophoresis. (wikipedia.org)
  • The blot is typically a first step in research, as it allows for the identification of the approximate molecular weights, once the molecular weight is known it allows for further research or purification through other methods like chromatography. (wikipedia.org)
  • size
  • Since none of the four nucleotide bases carry any charge, net charge becomes insignificant and size is the main factor affecting rate of diffusion through the gel. (wikipedia.org)
  • When the approximate size of the protein is known, the original sample can be run on a chromatography machine to separate it by size. (wikipedia.org)
  • page
  • Native-PAGE keeps the oligomeric form intact and will show a band on the gel that is representative of the level of activity. (wikipedia.org)
  • cells
  • After lysis of the cells (typically 1 to 2 hours at 4 °C) the slides are washed in distilled water to remove all salts and immersed in a second solution - an electrophoresis solution. (wikipedia.org)