• In the present study we applied gel electrophoresis with subsequent use of laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS), and tryptic digestion with subsequent analysis with high performance liquid chromatography (HPLC) coupled to both ICPMS and high-resolution tandem electrospray mass spectrometry (HR-ESI-MS) for the analysis of selenooproteins. (dtu.dk)
  • In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
  • Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
  • This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products. (elsevierpure.com)
  • Serum non-cholesterols sterols (NCSs), desmosterol, lathosterol, 7-dehydrocholesterol (7-DHC), campesterol and β-sitosterol were measured by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). (biochemia-medica.com)
  • Absolute quantification by mass spectrometry of HlgCB in 39 community-acquired pneumonia (CAP) isolates showed considerable variations in the HlgC and HlgB yields between isolates. (cnrs.fr)
  • Some proposals were additionally made to address the very common, significant research gaps noted in CECs research, especially the standardization of analytical chemical test methods, based on chromatography-mass spectrometry, for quantification. (openaccesspub.org)
  • Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. (cas.cz)
  • MudPIT) and Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) to identify potential biomarkers of PTL and SPTB. (elsevierpure.com)
  • Protein spots were resolved from three subcellular fractions, using two-dimensional differential-in-gel-electrophoresis, revealing 31 spots that changed significantly and were identified by liquid-chromatography tandem mass-spectrometry. (montana.edu)
  • The first goal of my project is developing a method for the analysis of oligonucleotides with nano liquid chromatography coupled to nano electrospray mass spectrometry (LC-ESI MS). Electrospray ionization is a soft ionization technique, so the fragile oligonucleotides don't decompose into many fragments. (ugent.be)
  • New advances in mass spectrometry-based proteomics technology are having amajor impact on our understanding of how human spermatozoa acquire their capacity for fertilization. (jri.ir)
  • Recently, use of mass spectrumetrybased (MS) proteomics technology has further contributed to the identification of the proteome that make up spermatozoa (6 - 12). (jri.ir)
  • A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS). (biomedcentral.com)
  • Proteomics relies mainly on mass spectrometry. (proprofs.com)
  • Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , 845 (1), 69-73. (elsevierpure.com)
  • Over the years, technologic advances in electronics and detectors have allowed GC/MS to detect ever smaller concentrations of compounds, as has the addition of other analytical techniques, such as liquid chromatography. (medscape.com)
  • With this, the purity or percentage composition of antimicrobial active compounds from silica gel can be determined. (encyclopedia.pub)
  • various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
  • This is based on thin-layer chromatography (TLC) performed on silica gel [ 13 ] . (encyclopedia.pub)
  • Active compounds are identified on the basis of their Rf values, while nonidentifiable active volatile compounds can be cut out from the silica gel and analyzed using the headspace solid-phase microextraction method coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS) [ 14 ] . (encyclopedia.pub)
  • Detailed protocols involving protein microarrays, liquid chromatography, high throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI TOF, and MudPIT are provided, along with well illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. (cshlpress.com)
  • Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel-eluted liquid fraction entrapment electrophoresis and (2) small-scale electrophoretic separation. (ulster.ac.uk)
  • GC/MS analysis allows separation of compounds based on their retention time within a chromatography column and identification of each compound by the characteristic fragments into which a given chemical is broken following ionization of the compound. (medscape.com)
  • This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. (bvsalud.org)
  • Two-dimensional gel electrophoresis in conjunction with liquid chromatography mass spectrometry analysis was utilized to identify accumbal protein candidates involved in the retrieval of cocaine-induced CPP. (tmu.edu.tw)
  • The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). (biomedcentral.com)
  • Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) data showed that bead beating and enzymatic lysis were the most efficient methods for analysis of the proteome. (qmul.ac.uk)
  • The trinitrobenzene sulfonic acid assay (TNBS) and denaturing gel electrophoresis were used to determine the extent of cross-linking. (cdc.gov)
  • Using native mass spectrometry, we identify the stoichiometry of sub-complexes and monitor oligomerisation of various assemblies. (nature.com)
  • MDI-Hb was also digested with trypsin and specific amino acid conjugation sites determined by ultra-performance liquid chromatography-quadruple-tandem time-of-flight mass spectrometry. (cdc.gov)