Sodium dodecyl sulfatePolyacrylamideInductively coupled plasHPLCProteinsDifference Gel ElectrophorQuantificationDifferential in-gel electrophoresisElectrospray mass spectroProteomicsAnalyticalAntimicrobialSilica gelMudPITSeparationAnalysisAssaySeraProtein sampleJournalIonizationHighIdentifyMethodsSamplesReferenceSpecific
Sodium dodecyl sulfate3
- These include liquid chromatography mass spectrometry along with sodium dodecyl sulfate polyacrylamide gel electrophoresis, or liquid chromatography mass spectrometry run in multiple dimensions. (wikipedia.org)
- The SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis revealed the presence of 34 and 43 kDa protein bands. (umk.pl)
- Proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were transferred onto a polyvinylidene difluoride membrane at 100 V for 1.5 hr. (cdc.gov)
Polyacrylamide6
- Proteins are commonly separated using two-dimensional polyacrylamide gel electrophoresis (2D PAGE). (wikipedia.org)
- A multidimensional differential proteomic platform using dual-phase ion-exchange chromatography-polyacrylamide gel electrophoresis/reversed-phase liquid chromatography tandem mass spectrometry. (ufl.edu)
- Serum low-density lipoprotein particles (LDL) and high-density lipoprotein particles (HDL) were separated using non-denaturing polyacrylamide gradient gel (3-31%) electrophoresis. (biochemia-medica.com)
- We investigated whether isopropanol extraction could facilitate resolution of the less abundant proteins from soybean seeds in one dimensional polyacrylamide gel electrophoresis (1D-PAGE) and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). (usda.gov)
- Milk proteins were determined by (1) two-dimensional gel electrophoresis and peptide mass mapping and (2) liquid chromatography-tandem mass spectrometry (LC-MS/MS) following one-dimensional polyacrylamide gel electrophoresis. (ulster.ac.uk)
- Individuals and Techniques: Two-dimensional polyacrylamide gel electrophoresis combined with liquid chromatography-tandem mass spectrometry was performed on 47 sera from wholesome volunteers, these with chronic active hepatitis, cirrhosis and HCC sufferers related with HBV and HCV infections. (glucagon-receptor.com)
Inductively coupled plas1
- In the present study we applied gel electrophoresis with subsequent use of laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS), and tryptic digestion with subsequent analysis with high performance liquid chromatography (HPLC) coupled to both ICPMS and high-resolution tandem electrospray mass spectrometry (HR-ESI-MS) for the analysis of selenooproteins. (dtu.dk)
HPLC4
- In the present study, cordycepin was prepared by preparative high‑performance liquid chromatography (prep-HPLC) and the purity obtained was 99.6%, indicating that this technique may be useful for the large‑scale isolation of cordycepin in the future. (spandidos-publications.com)
- Preparative high performance liquid chromatography (prep-HPLC) can shorten the production cycle, has relatively low operating costs, and is simple to carry out. (spandidos-publications.com)
- This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products. (elsevierpure.com)
- Serum non-cholesterols sterols (NCSs), desmosterol, lathosterol, 7-dehydrocholesterol (7-DHC), campesterol and β-sitosterol were measured by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). (biochemia-medica.com)
Proteins14
- For this technique, proteins are run across an immobile gel with a pH gradient until they stop at the point where their net charge is neutral. (wikipedia.org)
- Based on immunological and mass spectrometric analysis, SALSA was estimated to constitute up to 4-10% of the total protein amount in meconium, making it one of the most abundant proteins. (researchgate.net)
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the targets recognized by circulating AAbs seen on 2D-GE, followed by ELISAs with recombinant proteins to confirm LC-MS/MS results, and quantify autoreactivities. (duke.edu)
- Hong, SS, Park, JH & Kwon, SW 2007, ' Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry ', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol. 845, no. 1, pp. 69-73. (elsevierpure.com)
- The identification of proteins performed by using LC-MS/MS (liquid chromatography with tandem mass spectrometry) demonstrated their highest homology to bacterial porins. (umk.pl)
- We have identified a total of 107 protein spots using Matrix-assisted laser desorption/ionization time of flight mass spectrophotometry (MALDI-TOF-MS) and liquid chromatography mass spectrometry (LC-MS/MS). Our results suggest that extraction of soybean seed powder with 40% isopropanol enriches for lower abundance proteins and is a suitable method for 2D-PAGE separation and identification. (usda.gov)
- P 0.05), of which 42 spots that corresponded to 15 proteins had been identified by liquid chromatography-tandem mass spectrometry. (glucagon-receptor.com)
- Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. (cas.cz)
- Techniques used in human sperm proteome mapping: There are several initial reports using MS proteomics technology to identify a limited number of proteins from the human sperm using twodimensional gel electrophoresis (2-DE) coupled to MALDI-TOF-MS analysis (16 - 23). (jri.ir)
- An extensive human sperm proteome analysis using 1D-SDS-PAGE combined with electrospray liquid chromatography tandem mass spectrometry (GeLC-MS/ MS) approach identified 1,760 proteins (24). (jri.ir)
- Separation involves techniques such as gel electrophoresis or liquid chromatography to separate proteins based on their size, charge, or other properties. (proprofs.com)
- Identification is the process of determining the identity of the proteins, often done through mass spectrometry or protein sequencing. (proprofs.com)
- Mass spectrometry is a powerful technique used in proteomics to analyze and measure the mass of proteins. (proprofs.com)
- It helps in identifying proteins by measuring the mass-to-charge ratio of ions produced from the protein sample. (proprofs.com)
Difference Gel Electrophor1
- To discover such biomarkers, we measured AD-associated changes in the cerebrospinal fluid: CSF) using an unbiased proteomics approach: two-dimensional difference gel electrophoresis with liquid chromatography tandem mass spectrometry). (wustl.edu)
Quantification3
- Absolute quantification by mass spectrometry of HlgCB in 39 community-acquired pneumonia (CAP) isolates showed considerable variations in the HlgC and HlgB yields between isolates. (cnrs.fr)
- Some proposals were additionally made to address the very common, significant research gaps noted in CECs research, especially the standardization of analytical chemical test methods, based on chromatography-mass spectrometry, for quantification. (openaccesspub.org)
- Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. (cas.cz)
Differential in-gel electrophoresis2
- MudPIT) and Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) to identify potential biomarkers of PTL and SPTB. (elsevierpure.com)
- Protein spots were resolved from three subcellular fractions, using two-dimensional differential-in-gel-electrophoresis, revealing 31 spots that changed significantly and were identified by liquid-chromatography tandem mass-spectrometry. (montana.edu)
Electrospray mass spectro1
- The first goal of my project is developing a method for the analysis of oligonucleotides with nano liquid chromatography coupled to nano electrospray mass spectrometry (LC-ESI MS). Electrospray ionization is a soft ionization technique, so the fragile oligonucleotides don't decompose into many fragments. (ugent.be)
Proteomics4
- New advances in mass spectrometry-based proteomics technology are having amajor impact on our understanding of how human spermatozoa acquire their capacity for fertilization. (jri.ir)
- Recently, use of mass spectrumetrybased (MS) proteomics technology has further contributed to the identification of the proteome that make up spermatozoa (6 - 12). (jri.ir)
- A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography-tandem mass spectrometry (LC-MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS). (biomedcentral.com)
- Proteomics relies mainly on mass spectrometry. (proprofs.com)
Analytical2
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , 845 (1), 69-73. (elsevierpure.com)
- Over the years, technologic advances in electronics and detectors have allowed GC/MS to detect ever smaller concentrations of compounds, as has the addition of other analytical techniques, such as liquid chromatography. (medscape.com)
Antimicrobial1
- With this, the purity or percentage composition of antimicrobial active compounds from silica gel can be determined. (encyclopedia.pub)
Silica gel3
- various drawbacks have been identified in the purification techniques of ion resin adsorption ( 12 , 13 ), silica gel column chromatography ( 14 ) and supercritical extraction ( 15 ). (spandidos-publications.com)
- This is based on thin-layer chromatography (TLC) performed on silica gel [ 13 ] . (encyclopedia.pub)
- Active compounds are identified on the basis of their Rf values, while nonidentifiable active volatile compounds can be cut out from the silica gel and analyzed using the headspace solid-phase microextraction method coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS) [ 14 ] . (encyclopedia.pub)
MudPIT1
- Detailed protocols involving protein microarrays, liquid chromatography, high throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI TOF, and MudPIT are provided, along with well illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. (cshlpress.com)
Separation2
- Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel-eluted liquid fraction entrapment electrophoresis and (2) small-scale electrophoretic separation. (ulster.ac.uk)
- GC/MS analysis allows separation of compounds based on their retention time within a chromatography column and identification of each compound by the characteristic fragments into which a given chemical is broken following ionization of the compound. (medscape.com)
Analysis4
- This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. (bvsalud.org)
- Two-dimensional gel electrophoresis in conjunction with liquid chromatography mass spectrometry analysis was utilized to identify accumbal protein candidates involved in the retrieval of cocaine-induced CPP. (tmu.edu.tw)
- The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). (biomedcentral.com)
- Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) data showed that bead beating and enzymatic lysis were the most efficient methods for analysis of the proteome. (qmul.ac.uk)
Assay1
- The trinitrobenzene sulfonic acid assay (TNBS) and denaturing gel electrophoresis were used to determine the extent of cross-linking. (cdc.gov)
Sera1
- Based on these findings, sera were immunoprecipitated, and subjected to 2D gel electrophoresis (GE). (duke.edu)
Protein sample1
- Compared to simple 2D page, liquid chromatography mass spectrometry can handle a larger range of protein species size, but it is limited in the amount of protein sample it handle at once. (wikipedia.org)
Journal1
- Journal of Mass Spectrometry , 48 (7), 779-794. (ulster.ac.uk)
Ionization1
- 0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. (biomedcentral.com)
High1
- Defr1 fractions were collected by high-pressure liquid chromatography and analyzed by gel electrophoresis and mass spectrometry. (ed.ac.uk)
Identify1
- Using native mass spectrometry, we identify the stoichiometry of sub-complexes and monitor oligomerisation of various assemblies. (nature.com)
Methods1
- A variety of methods have been developed during the last two decades by using polymerase chain reaction (PCR) and gel electrophoresis. (ugent.be)
Samples1
- Saliva samples were submitted to two-dimensional electrophoresis (2DE). (biomedcentral.com)
Reference1
- Liquid chromatography mass spectrometry is also limited in its lack of a reference map from which to work with. (wikipedia.org)
Specific1
- MDI-Hb was also digested with trypsin and specific amino acid conjugation sites determined by ultra-performance liquid chromatography-quadruple-tandem time-of-flight mass spectrometry. (cdc.gov)