• The MTT, colony formation, flow cytometry, wound healing, transwell, and western blotting were performed to examine the function of miRNA-149 and FOXC1 in the development of gastric cancer. (hindawi.com)
  • Cell proliferation, migration, and invasion were measured with cell counting kit-8 (CCK-8), wound healing, and transwell assays. (jcancer.org)
  • Expression levels of Stathmin protein in each group were determined by using Western Blot, and the proliferation and migration ability of the cells with downregulated Stathmin were evaluated through CCK8 assay and transwell invasion assay, respectively. (biomedcentral.com)
  • Investigation the biological effects of DC-SIGNR were verified by MTT, wounding and transwell in vitro and experiments on animals to confirm gastric cancer liver metastasis by IVIS. (biomedcentral.com)
  • Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • Then, the relatively insensitive gastric cancer cell lines (MGC-803 and BGC-823) were treated with low concentrations of chidamide alone, bortezomib alone, or chidamide and bortezomib combination to detect the effects on cell proliferation, apoptosis, migration, and invasion. (dovepress.com)
  • Compared with low-dose chidamide or bortezomib alone, the low-dose drug combination significantly inhibited the proliferation, migration, and invasion of MGC-803 and BGC-823 cells and induced apoptosis of the cells. (dovepress.com)
  • Our results suggest that the combination of chidamide and bortezomib can significantly reduce the proliferation, invasion, and migration of MGC-803 and BGC-823 cells, providing a framework for the clinical evaluation of combined therapies for gastric cancers. (dovepress.com)
  • Furthermore, silencing PrP c expression with si-PRNP amplified the fucoidan-induced changes in cell proliferation, apoptosis, and migration. (iiarjournals.org)
  • In glioblastoma cells, the cell proliferation and migration were greatly inhibited after the downregulation of Stathmin protein. (biomedcentral.com)
  • Stathmin downregulation affected the biological behaviors of U373 and U87-MG glioblastoma cells, inhibiting the proliferation and migration of tumor cells. (biomedcentral.com)
  • Besides, it also participates in cell proliferation, differentiation, regeneration and motion via binding to different proteins [ 3 ]. (biomedcentral.com)
  • In the present study, we demonstrated that downregulation of Stathmin expression inhibited the cell proliferation, cell migration and tumor formation in nude mice of U373 and U87-MG glioblastoma cells. (biomedcentral.com)
  • So, Stathmin affects glioblastoma progression through regulating cell proliferation, cell cycle and cell migration. (biomedcentral.com)
  • Proteomic-based GO analysis demonstrated that biological processes such as cell deformation, proliferation, migration and wound healing occurred in the liver during the early infection. (biomedcentral.com)
  • DC-SIGNR knockdown inhibited the proliferation, migration and invasion of gastric cancer cells in vitro and suppressed the liver metastasis in vivo. (biomedcentral.com)
  • While, DC-SIGNR overexpression promoted cell proliferation, migration and invasion. (biomedcentral.com)
  • After infection, the expression of TLR2 protein was detected by Western blot, and cell proliferation and the cell cycle were detected by the CCK-8 method and fluorescence-activated cell sorting. (biomedcentral.com)
  • In an in vitro sporadic colorectal cancer cell experiment, TLR2 protein in the TLR2 knockdown group was significantly downregulated, and TLR2 knockdown significantly inhibited the proliferation of HCT116 and HT29 colorectal cancer cells, resulting in G1 phase arrest. (biomedcentral.com)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • MicroRNAs (miRNAs) are small endogenous RNAs that are associated with tumourigenesis, cell proliferation and apoptosis ( 4 , 5 ). (spandidos-publications.com)
  • SOX9 promotes nasopharyngeal carcinoma cell proliferation, migration and invasion through BMP2 and mTOR signaling. (cancerindex.org)
  • Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. (cancerindex.org)
  • CCK-8 assay was performed to examine cell proliferation. (biomedcentral.com)
  • Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • CC cell proliferation, invasion and migration induced by IL-17A significantly increased. (biomedres.info)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
  • CCK-8, plate colony formation and EdU assays were performed to assess the cell proliferation. (biomedcentral.com)
  • In addition, SNHG4 or RRM2 knockdown significantly induced cell cycle arrest and cell senescence, and inhibited DNA damage repair and cell proliferation, and the effects can be partially reversed by let-7a knockdown or RRM2 reoverexpression. (biomedcentral.com)
  • IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
  • The inhibitory effects of bufalin on cell proliferation were determined by MTT (Methyl thiazolyltetrazolium) assay. (biomedcentral.com)
  • 1) Bufalin inhibited the proliferation of SW620 cells. (biomedcentral.com)
  • Firstly, the overexpression of miRNA‑375 significantly suppressed cell proliferation, increased lactate dehydrogenase activity and induced apoptosis in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • HPV promotes latency and consequently does not cause a disease phenotype following initial infection by an immune evasion response reaction, featuring binding to epithelial cells and integrating into the host genome, resulting in the proliferation of epidermal cells containing the viral genome and neovascularization ( 3 ). (spandidos-publications.com)
  • However, the regulatory mechanisms of HPV infection and consequent immune escape reaction, persistent infection and cell proliferation and the regulatory mechanisms of vascular proliferation are poorly understood and require additional investigation ( 4 ). (spandidos-publications.com)
  • It affects cell proliferation, differentiation and inhibits apoptosis, and its role in tumor development has received attention ( 9 ). (spandidos-publications.com)
  • Overexpression of IGF-1 in serum and tissue alters the growth of normal cells and causes uncontrolled proliferation, inhibits differentiation and reduces apoptosis, resulting in the incidence and development of malignant tumors ( 10 ). (spandidos-publications.com)
  • In this study, we examined the effects of quercetin on cell cycle, viability, and proliferation of cancer cells, either singly or in combination with the microtubule-targeting drugs taxol and nocodazole. (vdocuments.net)
  • However, prolonged exposure of the cells to the highest protective dose of quercetin was still able to pre- vent cell proliferation. (vdocuments.net)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • A dual luciferase reporter assay was used to confirm the binding of miR‑132 and sirtuin 1 (SIRT1). (spandidos-publications.com)
  • Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
  • Luciferase reporter assay and RNA immunoprecipitation assay were performed to verify the RNA-RNA interactions. (biomedcentral.com)
  • 3′UTR luciferase reporter assay, RT-PCR, and Western blot were conducted to verify the target gene of miR-183. (molcells.org)
  • The 3′UTR luciferase reporter assay confirmed that miR-183 directly regulated PDCD4 by binding to sequences in the 3′UTR of PDCD4. (molcells.org)
  • Despite the short-term protection of cells by quercetin, colony formation and clonogenicity of HCT116 cells were still suppressed by quercetin or quercetin- taxol combination. (vdocuments.net)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
  • The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
  • This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
  • The in vitro I/R model was established by the hypoxia/reoxygenation method using H9C2 cells. (spandidos-publications.com)
  • We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • In vitro and in vivo experiments showed that SNHG4 overexpression markedly enhanced cell resistance to enzalutamide. (biomedcentral.com)
  • H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
  • C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)
  • In light of the advancements in tumor biology and molecular technology, further research into the molecular mechanism involved in gastric cancer and identification of biomarkers with high specificity and good targeting [ 3 ] is of great significance for the diagnosis and treatment of this condition. (hindawi.com)
  • Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
  • The effects of the low-dose chidamide and bortezomib combination reduced the growth on gastric cancer in vivo were investigated by using a subcutaneous tumor mouse model. (dovepress.com)
  • The antitumor activity and survival analysis of VALD-3 in vivo was determined by the nude mice xenograft assay. (researchsquare.com)
  • The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
  • In this ongoing work, we examined the impact of thioridazine on LCSCs world development, chemoresistance, cell cycle, and in vivo prohibitory function. (immune-source.com)
  • In vivo , orthotopic tumor xenografts assay was performed to investigate the role of C1QBP in RCC progression. (medsci.org)
  • Overexpression of Pim-1 in stem/progenitor cells stimulated cell cycling and enhanced cardiac regeneration in vivo. (biomedcentral.com)
  • Xenograft tumor assay was applied to detect the influence of EERAC on tumor growth. (jcancer.org)
  • In this study, the anti-tumor activity of DEH on colorectal cancer was investigated through both the cell-derived xenograft (CDX) and patient-derived tumor xenograft (PDX) model. (biomedcentral.com)
  • Background: The putative functions of the cellular prion protein (PrP c ) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. (iiarjournals.org)
  • Stathmin as a critical protein involved in microtubule polymerization, is necessary for survival of cancer cells. (biomedcentral.com)
  • According to the results from Western Blotting, Stathmin protein expression level decreased significantly in the U373 and U87-MG cells after transfected with pLV3-si-Stathmin, respectively, compared with those transfected with pLV3-NC. (biomedcentral.com)
  • Progress in understanding tumor stromal biology has been constrained in part because cancer-associated fibroblasts (CAF) are a heterogeneous population with limited cell-type-specific protein markers. (aacrjournals.org)
  • Western blot was used to detect the expression levels of p- NF-κβ, cyclin D1 and cyclin D3 protein in each group of cells. (biomedcentral.com)
  • The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. (cancerindex.org)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • Chromatin immunoprecipitation assay was performed to assess the bindings between protein and genomic DNA. (biomedcentral.com)
  • Mechanistic investigations revealed that through increasing the levels of intracellular reactive oxygen species (ROS), Gyp-L triggered protein ubiquitination and endoplasm reticulum (ER) stress response, leading to Ca 2+ release from ER inositol trisphosphate receptor (IP 3 R)-operated stores and finally cell death. (oncotarget.com)
  • Interestingly, there existed a reciprocal positive-regulatory loop between Ca 2+ release and ER stress in response to Gyp-L. In addition, protein synthesis was critical for Gyp-L-mediated ER stress and cell death. (oncotarget.com)
  • Real-time PCR and Western blot analyses were used to evaluate gene and protein expression levels. (wjgnet.com)
  • Background: This study aimed to investigate the efficacy and safety of chemotherapy combined with programmed cell death protein 1 (PD-1) inhibitors in the treatment of advanced cervical cancer and the effect of optimal combination timing on prognosis. (bvsalud.org)
  • The overexpression of miRNA‑375 significantly increased caspase‑3 and caspase‑9 activities, induced B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein, tumor protein 53 and cyclin‑dependent kinase inhibitor 1 protein expression and suppressed cyclin D1 and survivin protein expression in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • The overexpression of miRNA‑375 significantly suppressed the levels of protein expression of ubiquitin‑protein ligase E3A (UBE3A) and Insulin‑like growth factor‑1 receptor (IGF‑1R) in HPV‑18(+) cervical cancer cells. (spandidos-publications.com)
  • UBE3A is associated with cervical cancer and may combine with the E6 proto-oncogene encoded by HPV16 within cervical cancer cells to form the E6/E6-AP protein complex through the ubiquitin proteasome pathway ( 4 ). (spandidos-publications.com)
  • In addition, numerous important cellular proteins, such as B-cell lymphoma-2 homologous antagonist/killer, Myc proto-oncogene protein, cyclin-dependent kinase inhibitor 1B, DNA replication licensing factor MCM-7, retinoblastoma 1 and Annexin A1, are degenerated through the UBE3A-mediated ubiquitin proteasome pathway ( 7 ). (spandidos-publications.com)
  • Previously we have shown that DDB2, a protein involved in the Global Genome Repair, interacts directly with PCNA and, in human cells, the loss of this interaction affects DNA repair machinery. (biomedcentral.com)
  • To clarify this issue, we decided to apply a transfection-based assay, named Host Cell Reactivation (HCR), to investigate DNA lesions removal efficacy in the presence of DDB2 Wt protein or DDB2 mutated one. (biomedcentral.com)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • Tumor formation assay in nude mice showed that tumor formation was delayed after Stathmin downregulation, with a reduction in both tumor formation rate and tumor growth velocity. (biomedcentral.com)
  • Histone deacetylase (HDAC) plays a pivotal role in regulating the level of cellular DNA acetylation and participate in cell growth, differentiation, and apoptosis. (dovepress.com)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • This type of cancer usually results from continuous accumulation of genetic mutations in genes that control epithelial cell growth and differentiation and is often accompanied by inflammatory manifestations [ 4 , 5 ]. (biomedcentral.com)
  • Within the B-cell and T-cell categories, two subdivisions are recognized: precursor neoplasms, which correspond to the earliest stages of differentiation, and more mature differentiated neoplasms. (medscape.com)
  • Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
  • Numerous mechanisms such as acquisition of cancer stem cells (CSCs), a subpopulation characterized by self-renewal and differentiation abilities can explain HCC aggressiveness. (springer.com)
  • To date, combinational chemotherapy forms the main part of the treatment strategy for advanced gastric cancer with less than 10% of 5-year survival rate. (dovepress.com)
  • What is more, dual-luciferase assay and western blotting were used to demonstrated the relationship between miRNA-149 and FOXC1. (hindawi.com)
  • Studies [ 13 - 15 ] have shown that miR-149 plays an important role in many cancers, although its regulatory role in the FOXC1-mediated progression of gastric cancer remains unclear. (hindawi.com)
  • So, this study was designed to elucidate the function of Stathmin gene in the tumorigenesis and progression of glioblastoma cells. (biomedcentral.com)
  • IL-17A promotes the malignant progression of colon cancer cells by activating JAK2/STAT3 signaling pathway. (biomedres.info)
  • AG490 can significantly reverse the promoting effect of IL-17A on the malignant progression of CC cells. (biomedres.info)
  • There is also a complex link between IL-17A and CC. IL-17A promotes the cell cycle progression and tumor development of CC cells by membrane-bound form [ 10 ]. (biomedres.info)
  • This antagonism of microtubule-targeting drugs was accompanied by a delay in cell cycle progression and inhi- bition of the buildup of cyclin-B1 at the microtubule organizing center of treated cells. (vdocuments.net)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • Furthermore, in silico analysis from The Cancer Genome Atlas (TCGA) database suggests that overexpression of ALKBH6 provides better survival outcomes in patients with pancreatic cancer. (frontiersin.org)
  • In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
  • Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
  • The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
  • Meanwhile, EERAC remarkably reversed the effects of mastermind like transcriptional coactivator 1 (MAML1, activator of notch-signaling pathway) on cell survival of SW480. (jcancer.org)
  • Univariate Cox regression analysis showed that Annexin A2 and S100A10 in stromal tissue correlated with shorter overall survival (OS). (cancerindex.org)
  • In addition, survival analysis results showed that VALD-3 can significantly prolong the survival time of mice ( P =0.036). (researchsquare.com)
  • 1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
  • We proposed that hypoxic preconditioning could increase survival of bone marrow mesenchymal stem cells (MSCs) via upregulation of Pim-1 and aimed to determine the microRNAs that modulate the expression of Pim-1. (biomedcentral.com)
  • 9 ] reported that hypoxic preconditioning increases survival of cardiac progenitor cells via upregulation of Pim-1, discovering a method for nongenetic modification of Pim-1 in stem cells. (biomedcentral.com)
  • Stathmin is a key regulator of the signaling pathway, thus affecting the cell growth. (biomedcentral.com)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • The purpose of the research is to investigate the roles of Jak-STAT3 signaling pathway in bufalin-induced apoptosis in colon cancer SW620 cells. (biomedcentral.com)
  • Therefore, investigating the expression and regulation of miRNAs may elucidate the mechanism of gastric cancer. (hindawi.com)
  • AHCC mechanism of action was evaluated with immunoblotting and flow cytometry studies. (researchgate.net)
  • The antitumor effect of ethanol extracted from radix of Actinidia chinensis (EERAC) were identified in human colon cancer cells, but the underlying mechanism remains unclear. (jcancer.org)
  • Insights of the mechanism were employed microarray and bioinformatic analysis. (biomedcentral.com)
  • Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
  • The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
  • RNA stability assay was applied to characterize the mechanism of C1QBP regulating XDH transcription. (medsci.org)
  • To conclude, it was identified that the probable anti‑CA mechanism of miRNA‑375 modulates HPV through the UBE3A and IGF‑1R pathway in cervical cancer cells. (spandidos-publications.com)
  • Further to confirm the results were conducted by qRT-PCR, western blot and by flow cytometry. (biomedcentral.com)
  • Western blot analysis revealed that VALD-3 upregulated pro-apoptotic proteins (Bad and Bax), downregulated anti-apoptotic proteins (Bcl-2, Bcl-xl, survivin and XIAP) and increased the expression of cleaved caspase-3, cleaved caspase-8, Cyto-c and cleaved PARP. (researchsquare.com)
  • Western blot analysis showed that the expression of programmed cell death 4 (PDCD4), which was predicted as the target gene of miR-183 by microarray profiling and bioinformatics predictions, decreased when miR-183 was over-expressed. (molcells.org)
  • Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
  • Olga held the position of Director of Bioassay Development at DVS Sciences, Inc. After the merger with Fluidigm Inc in 2014, she transitioned to Principal Scientist, Proteomics division, and lead a group of biology and chemistry researchers involved in developing new metal-tagged affinity reagents, as well as methods and applications for Mass cytometry until her retirement in 2019. (wikipedia.org)
  • Materials and Methods: We used a previously reported two-step emulsification method to obtain poly (lactic-co-glycolic acid) /GA nanoparticles (PLGA/GA NPs), and then CT26 colon cancer cell membrane (CCM) was used to obtain CCM-PLGA/GA NPs. (bvsalud.org)
  • However, methods for utilizing Annexin V for flow cytometry on adherent cell types have been previously reported by Casiola-Rosen et al. (aatbio.com)
  • It is difficult to diagnose owing to non-specific clinical manifestations, the serologic index, imaging features, and the limitations of diagnostic methods. (frontiersin.org)
  • In certain circumstances, when a lymph node is not easily accessible for excisional or incisional biopsy, a combination of core biopsy and FNA biopsies in conjunction with appropriate ancillary techniques for the differential diagnosis (immunohistochemistry, flow cytometry, PCR for IGHV and TCR gene rearrangements, karyotype, and fluorescence in situ hybridization [FISH] for major translocations) may be sufficient for diagnosis. (medscape.com)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • Senescence-associated β-Gal assay was used to detect the cell senescence level. (biomedcentral.com)
  • Furthermore, knockdown of the Stathmin expression also induced the cell cycle arrest at G2/M phase, but no significantly statistical difference on apoptosis rate in U373 and U87-MG cells. (biomedcentral.com)
  • Knockdown of PERK or IRE1α significantly decreased DEH-induced autophagy and retrieved cell viability in cells treated with DEH. (biomedcentral.com)
  • The expression levels of p-NF-κβ, cyclin D1 and cyclin D3 proteins in TLR2 gene knockdown group cells were significantly reduced. (biomedcentral.com)
  • The cells were treated with different dosage of thioridazine and cell viability was assessed 2 days later or cells were treated with 10? (immune-source.com)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • However, DC-SIGNR as a family member of selectin participates in gastric cancer liver metastasis remains unknown. (biomedcentral.com)
  • DC-SIGNR promoted gastric cancer liver metastasis mediated with HNRNPKP2 which expression was regulated by STAT5A. (biomedcentral.com)
  • These findings indicated potential therapeutic candidates for gastric cancer liver metastasis. (biomedcentral.com)
  • Despite surgical resection and chemoradiotherapy can control most cancer cells [ 5 ], a surgical resection has been rarely indicated for liver metastasis from gastric cancer [ 6 ]. (biomedcentral.com)
  • In experimental metastasis studies, researchers demonstrate that liver sinusoidal endothelial cell lectin (LSECtin) mediated colon cancer cells metastasis to liver displays enhanced abilities to the specific organ [ 8 ]. (biomedcentral.com)
  • Also, serum of soluble E-selectin (sE-selectin) concentration in gastric cancer patients are detected by ELISA, but increasing only in gastric cancer patients with peritoneal metastasis [ 9 ]. (biomedcentral.com)
  • Here, we ask whether DC-SIGNR contributes to hematogenous metastasis from gastric carcinoma. (biomedcentral.com)
  • Recently, several long ncRNAs have been reported to have a role in gastric cancer metastasis. (biomedcentral.com)
  • Lung tumor stem cells are a subpopulation of cells important for lung tumor development, metastasis, and medication resistance. (immune-source.com)
  • TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
  • we found that EERAC inhibited the cell viability, migration, and invasion of SW480 cells in a concentration dependent manner, but promoted apoptosis and the cell percentage in S phase significantly. (jcancer.org)
  • IL-17A is released by tumor-associated neutrophils and promotes the migration and invasion of gastric cancer cells [ 7 ]. (biomedres.info)
  • CAE induced a dose-dependent increase in cell migration and invasion and also in the expression of cancer stem cells markers (CD133, CD44, CD90). (springer.com)
  • Several alkylating agents are commonly used in chemotherapy but may induce DNA damage to cancer cells as well as normal cells. (frontiersin.org)
  • But Stathmin downregulation did not induce significant cell apoptosis. (biomedcentral.com)
  • Myocardial I/R injury may induce cell apoptosis and autophagy by activating oxidative stress and upregulating inflammatory mediators, ultimately resulting in irreversible fibrotic damage ( 3 ). (spandidos-publications.com)
  • Hence, it is of great importance to investigate the mechanisms that induce enzalutamide resistance in prostate cancer cells. (biomedcentral.com)
  • Treat cells with test compounds for a desired period of time (4-6 hours for Jurkat cells treated with staurosporine) to induce apoptosis. (aatbio.com)
  • Al- though quercetin induced cell death in a dose-dependent manner, 12.5-50 µ M quercetin inhibited the activity of both taxol and noco- dazole to induce G2/M arrest in various cell lines. (vdocuments.net)
  • Her primary field of expertise is in cellular and molecular biology, with the objective of developing bioanalytical assays for mass cytometry(CyTOF). (wikipedia.org)
  • Moreover, DEH induced strong cellular autophagy, which could be inhibited through autophagic inhibitors, with a rction in the DEH-induced inhibition of cell growth in colorectal cancer cells. (biomedcentral.com)
  • Once produced within a cell, free radicals can damage a wide variety of cellular constituents, including proteins, lipids and DNA. (enzolifesciences.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Twenty pairs of gastric cancer and adjacent nontumor tissues, which were not subjected to radiotherapy or chemotherapy, were obtained from the First Affiliated Hospital of Xi'an Jiaotong University. (hindawi.com)
  • In the present study, up-regulation of SOX9 was observed in both NPC tissues and different NPC cells. (cancerindex.org)
  • In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
  • Correlation analysis was also used to compare the deletion of four MMR proteins in CRC tumor tissues. (bvsalud.org)
  • TLR2 was localized in the cell membrane of the colorectal epithelium of the NC group and of the colorectal tumors of the NT group and was highly expressed in the NT group, while antigen Ki67 was localized in the nucleus of the colorectal tumor cells of the NT group and the KT group, and its expression was reduced in the KT group. (biomedcentral.com)
  • Consensus clustering analysis identified two distinct clusters with different expression profiles of membrane trafficking-related genes. (bvsalud.org)
  • APC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. (aatbio.com)
  • Annexin V flow cytometric analysis on adherent cells is not routinely tested since specific membrane damage may occur during cell detachment or harvesting. (aatbio.com)
  • In addition, gain and loss-of-function studies with miR-206 mimics and inhibitors showed that inhibition of miR-206 in hypoxic MSCs promoted the migration ability of the cells, prevented cell apoptosis, and protected membrane potential of mitochondria, while the benefits were all blocked by Pim-1 inhibitor. (biomedcentral.com)
  • Gastric cancer is one of the most common cancers in the world. (hindawi.com)
  • Actinidia chinensis has presented promising therapeutic effect on several types of cancers including breast, liver, and gastric cancer [ 10 , 11 ]. (jcancer.org)
  • They are named tumor control cancers or cells initiating cells [4]. (immune-source.com)
  • After that, tumor control cells had been reported in different types of malignancies, including lung tumor, gastric tumor, human brain cancers, liver organ cancers, and digestive tract cancers [7C11]. (immune-source.com)
  • After the breakthrough discovery of tumor control cells, analysts attempted to appearance for effective techniques to focus on cancers control cells. (immune-source.com)
  • Renal cell carcinoma is one of the most common cancers in the urinary system, causing over 140,000 deaths worldwide each year[ 1 ]. (medsci.org)
  • In addition, the KEGG analysis of multi-omics data demonstrated that numerous pathways related to immunity, inflammation, tumorigenesis and metabolism were enriched in the liver. (biomedcentral.com)
  • Our findings indicated that treatment with DEH arrested the cell cycle of colorectal cancer cells at the G1/S phase, leading to significant inhibition in cell growth. (biomedcentral.com)
  • Lentivirus carrying TLR2-RNAi was used to stably infect colorectal cancer cells (HCT116 and HT29) to knock down TLR2 gene expression. (biomedcentral.com)
  • Colorectal mucosal cells FHC cells and colorectal cancer cells HT29 and HCT116 obtained from Bena Culture Collection (Hebei China) were cultured in RPMI 1640 medium with 10% FBS at 37°C in 5% CO 2 . (biomedres.info)
  • Several studies have confirmed that miRNA can be used as an oncogene or tumor suppressor gene to regulate the occurrence and development of gastric cancer [ 6 ]. (hindawi.com)
  • The lentiviral interference vector pLV3-si-Stathmin targeting Stathmin gene and the control vector pLV3-NC were established for the co-transfection of 293T cells together with the helper plasmids. (biomedcentral.com)
  • The other approach is to screen CRISPR-Cas9-based guide RNA (gRNA) libraries that target either the whole genome using cancer cell and immune cell co-culture systems 9 , 10 , or focused gene sets using immunocompetent murine tumors 11 , 12 . (nature.com)
  • We explored the oncogenic role of miR-183 in ESCC and identified programmed cell death 4 (PDCD4) as a direct target gene of miR-183. (molcells.org)
  • Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. (thermofisher.com)
  • This format retains its high affinity for phosphatidylserine (PS) and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. (aatbio.com)
  • Conclusion: Combination of fucoidan with silencing of PrP c has a synergic effect on the inhibition of HT29 colon cancer cell growth. (iiarjournals.org)
  • It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)
  • Gyp-L-induced cell death was associated with lysosomal swelling and autophagic flux inhibition. (oncotarget.com)
  • The aim of this study was to investigate the antitumor effect of chidamide in combination with bortezomib on gastric cancer cell lines. (dovepress.com)
  • Normal human colon epithelial cell NCM460, human colorectal cancer cell lines (HCT 116 and SW620) and human embryonic renal cell lines (293 FT) were purchased from the American Type Culture Collection (ATCC, USA) and stored in our laboratory. (biomedcentral.com)
  • The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • miRNA-149 inhibits the biological behavior of gastric cancer by targeting FOXC1, providing a promising target in the treatment of human gastric cancer. (hindawi.com)
  • The expression of IL-4 and IL-17A has been shown to be significantly increased in human pancreatic cancer cells and synergistically promotes the production of hydrogen peroxide, causes DNA oxidative damage and increases the expression of dual oxidase 2 [ 9 ]. (biomedres.info)
  • Furthermore, analysis of liver transcriptome, proteome and metabolome changes was performed using multi-omics techniques. (biomedcentral.com)
  • Histopathological analysis revealed that liver injury, characterized by collagen deposition and inflammatory cell infiltration, occurred as early as 24 h of infection. (biomedcentral.com)
  • Furthermore, proteomic and transcriptomic analysis jointly verified that the detoxification and antioxidant defense system was activated by enrichment of glutathione metabolism and cytochrome P450-related pathways in response to acute liver injury. (biomedcentral.com)
  • Similarly, hepatic sinusoidal endothelial E-selectin expression is up regulated by highly metastatic cells entering the liver [ 10 ]. (biomedcentral.com)
  • Early stage Huh-7 and advanced SNU449 liver cancer cell lines were subjected to chronic alcohol exposure (CAE), at different doses for 6 months followed by 1-month alcohol withdrawal period. (springer.com)
  • Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. (iiarjournals.org)
  • Correspondingly, transcriptomic results showed significant enrichment of cell cycle pathway on day 3 and 7. (biomedcentral.com)
  • Fortunately, after treatment with amphotericin B as an antifungal, the patient's symptoms, inflammatory markers, and imaging results showed significant improvement. (frontiersin.org)
  • The results have shown that DDB2 Wt recognize and repair the UV-induced lesions in plasmidic DNA transfected in the cells, whereas a delay in these processes were observed in the presence of DDB2 PCNA- , as also confirmed by the different extent of co-localization of DDB2 Wt and some NER proteins (such as XPG), vs the DDB2 mutant form. (biomedcentral.com)
  • These results suggest the use of cell free DNA in plasma to assess EGFR mutation status may be a feasible alternative in those patients for whom a tumor biopsy cannot be obtained. (selectbiosciences.com)
  • We will show analyses results as well as assay development process leading to biomarker validation. (selectbiosciences.com)
  • Functional analysis of differentially expressed genes between the two clusters revealed that MTRGs were involved in immune response and metabolic processes, and a risk signature model based on MTRGs was established to predict the prognosis of CRC patients. (bvsalud.org)
  • DC-SIGNR serum level was significantly increased in gastric cancer patients compared with healthy group. (biomedcentral.com)
  • The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
  • Taken together, this work suggested a novel therapeutic option by Gyp-L through the induction of an unconventional ROS-ER-Ca 2+ -mediated cell death in human esophageal cancer. (oncotarget.com)
  • Further analysis indicated that DEH also induced endoplasmic reticulum (ER) stress and subsequently stimulated autophagy through the activation of PERK/eIF2α and IRE1α/XBP-1 s/CHOP pathways. (biomedcentral.com)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
  • Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)
  • CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
  • This is certainly the initial function on tumor control cells in solid growth [6]. (immune-source.com)
  • Bufalin not only inhibited the growth of colon cancer SW620 cells, but also induced apoptosis of SW620 cells. (biomedcentral.com)
  • The status of cell adherence to growth ma- trix was critical in determining the sensitivity of HCT116 cells to quercetin. (vdocuments.net)
  • Thus, we identify bimodal activity of the flavonoid quercetin, a short-term activity that is cytoprotective against chemotherapeutic drugs and a long-term activity that is inhibitory to cancer cell growth. (vdocuments.net)
  • First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
  • We find that the core microRNA (miRNA) biogenesis and targeting machinery maintains the sensitivity of cancer cells to PD-1-independent T cell-mediated cytotoxicity. (nature.com)
  • Two complementary approaches have been adopted to identify genetic elements that control cancer cell sensitivity to T cell-mediated cytotoxicity. (nature.com)
  • LRRC15 was expressed on stromal fibroblasts in many solid tumors (e.g., breast, head and neck, lung, pancreatic) as well as directly on a subset of cancer cells of mesenchymal origin (e.g., sarcoma, melanoma, glioblastoma). (aacrjournals.org)
  • In the latest years, people discovered that there is certainly a little subpopulation of cells in tumors, which play a essential function in the resistance of cancer to radiotherapy and chemotherapy. (immune-source.com)
  • cells could initiate tumors in rodents. (immune-source.com)
  • With the development of tumor molecular heterogeneity theory(12), there are still some patients with GIST who are sensitive to radiotherapy, especially for the patients with advanced stage(13-16).In addition, with the development of imaging technology and modern tissue and organ radiation technology, it has become a reality to concentrate high-dose radiation locally in abdominal cavity tumors(12), which challenges RT's insensitivity to GISTs. (researchsquare.com)
  • The lack of claudin-4 expression in ovarian small cell carcinomas of hypercalcemic type suggests that these tumors may be better regarded as sarcomas rather than carcinomas. (cusabio.cn)
  • Effective immunotherapy of stromal-rich tumors requires simultaneous targeting of cancer cells and immunosuppressive elements of the microenvironment. (aacrjournals.org)
  • The incidence rate of gastric cancer is fifth in the world, and the mortality rate is third, second only to lung cancer and colorectal cancer [ 1 ]. (hindawi.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
  • Cancer cells acquire genetic heterogeneity to escape from immune surveillance during tumor evolution, but a systematic approach to distinguish driver from passenger mutations is lacking. (nature.com)
  • Genetic inactivation of the machinery or re-introduction of ANKRD52 frequent patient mutations dampens the JAK-STAT-interferon-γ signaling and antigen presentation in cancer cells, largely by abolishing miR-155-targeted silencing of suppressor of cytokine signaling 1 (SOCS1). (nature.com)
  • Our data indicate that the evolutionarily conserved miRNA pathway can be exploited by cancer cells to escape from T cell-mediated elimination and immunotherapy. (nature.com)
  • Cancer immunotherapies such as immune checkpoint blockade (ICB) unleash T cell cytotoxicity against cancer cells and have significantly improved the perspective of cancer patients. (nature.com)
  • It is therefore imperative to uncover novel immune evasion mechanisms in the dynamic tumor-immune microenvironment by untangling the genetic heterogeneity of cancer cells. (nature.com)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • In this study, we show that treatment with gypenoside L (Gyp-L), a saponin isolated from Gynostemma pentaphyllum , induced nonapoptotic, lysosome-associated cell death in human esophageal cancer cells. (oncotarget.com)
  • We examined the effect of the cotreatment of cancer cells with the flavonoid quercetin and 2 antimicrotubule drugs, namely, taxol and nocodazole. (vdocuments.net)
  • DNA sequencing and viral titer assay indicated that the lentiviral interference vector was successfully established with a viral titer of 4 × 10 8 TU/ml. (biomedcentral.com)
  • In 1989, she completed her Ph.D. in Cell & Molecular Biology, and worked as a Research Scientist at the Cardiology Centre studying vascular smooth muscle involvement in atherosclerosis. (wikipedia.org)
  • The ROS-ID ® ROS/RNS detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. (enzolifesciences.com)