• Finally, a clinical Lactobacillus crispatus isolate (24-9-7) produces lactic acid and inhibits growth of both G. vaginalis and N. gonorrhoeae on PVM. (biomedcentral.com)
  • Therefore, we quantified adherence of G. vaginalis and other BV-associated bacteria to an inert surface pre-coated with Lactobacillus crispatus using a new Peptide Nucleic Acid (PNA) Fluorescence In Situ Hybridization (FISH) methodology. (mdpi.com)
  • 23 Based on the mean log concentration, Lactobacillus was present at a concentration 10 times greater, at log 10 7 levels, than the concentration of the next microorganism, Gardnerella vaginalis , present at log 10 6 levels in about one half the patients. (glowm.com)
  • In an effort to better understand the differences between G. vaginalis isolated from women with a positive (BV) versus a negative (non-BV) diagnosis of BV, we compared the virulence potential of 7 BV and 7 non-BV G. vaginalis isolates and assessed the virulence factors related to biofilm formation, namely: initial adhesion and cytotoxic effect, biofilm accumulation, susceptibility to antibiotics, and transcript levels of the known vaginolysin, and sialidase genes. (ualg.pt)
  • G. vaginalis isolates should be incubated in a 5-10% CO 2 enriched atmosphere for best results. (hardydiagnostics.com)
  • The genetic characterisation of T. vaginalis isolates reveals significant genetic diversity in this organism. (hindawi.com)
  • Gardnerella vaginalis (ATCC ® 14018) colonies growing on Starch Agar with Bromcresol Purple (Cat. (hardydiagnostics.com)
  • BV was detected with the Nugent scoring system, and A. vaginae and G. vaginalis DNA was quantified with a multiplex quantitative real-time PCR assay. (bmj.com)
  • T. vaginalis was detected using the TaqMan assay using commercially available primers and probes specific for this protozoan (Pr04646256_s1). (hindawi.com)
  • Increasing levels of G. vaginalis and M. hominis and decreasing levels of lactobacilli were significantly associated with BV by Nugent score. (pubmedcentralcanada.ca)
  • Of the group with Nugent scores of 7 to 10, 83% and 81% had log 10 G. vaginalis counts and log 10 M. hominis counts greater than 6.81 and 4.82, respectively, while only 30% and 31% of the group with Nugent scores of 0 to 3 were above these thresholds, respectively. (pubmedcentralcanada.ca)
  • PCR quantification of G. vaginalis and M. hominis from CVL is significantly more sensitive than Amsel criteria for diagnosing BV. (pubmedcentralcanada.ca)
  • Gardnerella vaginalis hemolysin: Isolation, characterization and in vitro effects on polymorphonuclear leukocytes. (edu.ps)
  • There appeared to be a relationship between patients who presented with vaginal discharge with isolation of G. vaginalis. (edu.ng)
  • Microbiology of vaginal discharge with emphasis on gardnerella vaginalis. (ukzn.ac.za)
  • G. vaginalis is a fastidious organism and requires complex medium for growth. (biomedcentral.com)
  • This phenomenon could have important implications regarding colonization of the vagina by G. vaginalis and may suggest an explanation for the lack of an overt immune response to this organism. (asm.org)
  • G. vaginalis was isolated from 51.3 percent of the total population using a highly selective medium that detected the organism in lower concentration in vaginal discharge than did previously used media. (nih.gov)
  • The probe system detected G. vaginalis in 57 (90%) of 63 vaginal specimens from women having clue cells on wet mount examination, and in only 3 (3%) of 113 women without clue cells, suggesting that the Affirm probe for G. vaginalis could be used as a surrogate for wet mount examination for clue cells. (asm.org)
  • Vaginal wet smear microscopy detected T. vaginalis in 29% and "clue" cells in 41,3% of smears. (ukzn.ac.za)
  • To support further studies aimed at understanding the role of G. vaginalis in BV, we sequenced the genomes of 15 G. vaginalis strains (including JCP8151B) that were isolated from the vaginal swabs from 12 women enrolled in the institutional review board (IRB)-approved Washington University Contraceptive CHOICE Project ( 13 ). (asm.org)