Gaps hydrophobic residues in protein protein residues. Medical search. Frequent questions

The ARF-GAP domain comprises a central three-stranded beta-sheet flanked by five alpha-helices, with a Zn(2+) ion coordinated by the four cysteines of the cysteine-rich motif. (embl.de)
Protein prenylation is the posttranslational attachment of either a farnesyl group or a geranylgeranyl group via a thioether linkage (-C-S-C-) to a cysteine at or near the carboxyl terminus of the protein. (biomedcentral.com)
There are three different protein prenyltransferases in humans: farnesyltransferase (FT) and geranylgeranyltransferase 1 (GGT1) share the same motif (the CaaX box) around the cysteine in their substrates, and are thus called CaaX prenyltransferases, whereas geranylgeranyltransferase 2 (GGT2, also called Rab geranylgeranyltransferase) recognizes a different motif and is thus called a non-CaaX prenyltransferase [ 1 ]. (biomedcentral.com)
a thioester connection to a cysteine within a proteins. (epf2013.org)
The electrophoretic buffers.Studies of numerous peptides the presence of basic residues (arginine and lysine), and perhaps even the presence of a neighboring cysteine. (epf2013.org)
The invention relates generally to antibodies engineered with reactive cysteine residues and more specifically to antibodies with therapeutic or diagnostic applications. (justia.com)
2011). Cysteine scanning mutagenesis (residues Glu52-Gly96) of the human P2X1 receptor for ATP: mapping agonist binding and channel gating. (gozmusic.org)

d ) A sectional view through the map and superimposed model, showing details of the central transmembrane pore (functionally important amino acid residues on the α -subunit pore-lining M2 helices, 9′Leu, uppermost, and −1′Glu, are shown in red). (iucr.org)
Suppose I start with a given polypeptide sequence M at time t, and observe the evolutionary changes in the sequence until 1 of all amino acid residues have undergone substitutions at time tn. (progsound.de)

Sequences of ARF-GAP domains show no recognizable similarity to those of other GAPs, and contain a characteristic Cys-X(2)-Cys-X(16-17)-Cys-X(2)-Cys motif. (embl.de)
Pauling believed that protein shapes-independently of amino acid sequences-determined antibody specificity and biological specificity in general. (ucpress.edu)
When the number of amino acids is less than about 50 these molecules are named peptides while larger sequences are referred to as proteins. (powerandbulk.com)
The telomeres consist of repeating sequences and a set of special proteins, which interact with these repeats and spatially organize them in a specific manner, resulting in the formation of the nucleoprotein complex known as telomeric heterochromatin [ 6 , 7 ]. (actanaturae.ru)
Phylogenetic analysis of plant MIP sequences revealed four major subfamilies: the plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like intrinsic proteins (NIPs) and small and basic intrinsic proteins (SIPs). (biomedcentral.com)
What is the difference between them and which version of them should I use How do I know which substitution matrix to use in my algorithm when trying to align protein sequences. (progsound.de)
DNA substitution matrices DNA is less conserved than protein sequences. (progsound.de)
Furthermore, against the backdrop that the number of well-studied proteins continues to grow at a slow rate, it necessitates for a search methodology to dive deeper into the sequence similarity space to connect the unknown sequences to the well-studied ones, albeit more distant, for biological function postulations. (beds.ac.uk)
Implicitly, this refers to a high level of similarity among comparable structural elements across the sequences so that a common structural fold among these homologs is maintained which, in turn, governs the general biological function of this homologous protein family. (beds.ac.uk)
Predicting protein properties from amino acid sequences is an important problem in biology and pharmacology. (bvsalud.org)
We compare the effectiveness of these models in predicting the binding affinity and expression of SARS-CoV-2 spike protein sequences. (bvsalud.org)
We also explore how effective these predictive methods are when trained on laboratory-created data and are tasked with predicting the binding affinity of the in-the-wild SARS-CoV-2 spike protein sequences obtained from the GISAID datasets. (bvsalud.org)
We observe that TR is a better method when the sample size is small and test protein sequences are sufficiently similar to the training sequence. (bvsalud.org)
A proportion of these will alter protein sequences, the most common of which is the non-synonymous single nucleotide polymorphism (nsSNP). (biomedcentral.com)

The aggregated views MISA/SSE, MISA/BSA, MISA/ΔASA, and so forth, make it trivial to identify commonalities and differences between chains, to infer key interface residues, and to understand where conformational changes occur upon binding. (bvsalud.org)

Recent advances in drug design have led to the development of several classes of novel therapeutic macromolecules including peptides, proteins, monoclonal antibodies, immunotoxins, lysozymes, plasmid DNA, antisense oligodeoxynucleotides, and short interfering RNA. (usc.edu)
In biological macromolecules like proteins, the hydrophobic residues tend to clump together in the interior of the folded structure. (cheap-essay.org)
In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)

They occur in a large number of functionally diverse proteins mainly from eukaryotes. (embl.de)
The α subunits of protein prenyltransferases consist of tetratricopeptide repeats and are part of the tetratricopeptide repeat superfamily [ 5 ], which also includes functionally diverse proteins involved in transcription, co-chaperoning, protein transport, cell-cycle control and phosphorylation. (biomedcentral.com)

Deciphering protein–protein interactions. (crossref.org)
They are involved in protein-protein interactions. (embl.de)
The ParB protein forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for segregation. (elifesciences.org)
Our crystal structures show that, in transforming mutants, switch II takes part in a network of hydrophobic interactions burying the nucleotide and precatalytic water molecule. (ncsu.edu)
Snorkeling and anchoring interactions of Arg-44 and Tyr-60, respectively, with the polar region or polar hydrophobic interface of the lipid bilayer are suggested as control elements for insertional depth and orientation of the helix in the lipid matrix. (shengsci.com)
Small residues are group-conserved in the helix-helix interface of MIP structures and they seem to be important for close helix-helix interactions. (biomedcentral.com)
Protein-protein interactions among SARS-CoV-2 spike protein, human receptors and antibodies are key determinants of the potency of this virus and its ability to evade the human immune response. (bvsalud.org)
For over 40% of the nsSNPs, structure-based methods predicted which of these sequence changes are likely to either disrupt the structure of the protein or interfere with the function or interactions of the protein. (biomedcentral.com)
To make proteins, such as binding, recognition and catalysis, often progress, it is therefore helpful to adopt a sceptical atti- involve specific interactions with individual water mol- tude. (lu.se)

CRIP1a exhibits a 10-stranded and antiparallel ß-barrel with an interior comprised of conserved hydrophobic residues and loops at the bottom and a short helical cap at the top to exclude solvent. (bvsalud.org)
An invariant arginine and several nearby hydrophobic residues are solvent exposed and are predicted to be the site of interaction with ARFs. (embl.de)
The second step involves the transfer of a proton from the zinc-bound water to bulk solvent, regenerating the zinc-bound hydroxide (equation 2, where B stands for a general base: either a water or a proton-shuttling residue). (iucr.org)
Abstract The multiple solvent crystal structures (MSCS) method uses organic solvents to map the surfaces of proteins. (ncsu.edu)
Conversely, the deuterated Ala-63 sample revealed a peak centered at 0 kHz with a linewidth of 1.9 kHz, indicating increased side-chain motion and solvent exposure relative to the spectra of the other Ala residues. (shengsci.com)

Methoxine incorporation in recombinant proteins is likely to occur when an excess of methanol is present during fermentation but can be avoided when the methanol feed rate protocol is carefully designed. (biomedcentral.com)
The promoter of the AOX1 gene has become a popular tool to drive the expression of recombinant proteins. (biomedcentral.com)
To gain insight into the intracellular fluxes of subpopulations and their interplay within such mixed cultures, we developed here a (13)C flux analysis approach based on affinity purification of the recombinant fusion glutathione S-transferase (GST) and green fluorescent protein (GFP) as a reporter protein. (gozmusic.org)

Snake venoms are a complex mixture of peptides and proteins, including peptidases. (biomedcentral.com)
The consequential perturbations provide a useful tool for chemical biologists to reveal new structure-function relationships, and to endow peptides and proteins with desirable attributes. (pdffox.com)

Lysine Exporters are a superfamily of transmembrane proteins which export amino acids, lipids and heavy metal ions. (wikipedia.org)
The superfamily was named based on the early discovery of the LysE carrier protein of Corynebacterium glutamicum. (wikipedia.org)
ADP ribosylation factors (ARFs), which are members of the Ras superfamily of GTP-binding proteins, are critical components of vesicular trafficking pathways in eukaryotes. (embl.de)
Aquaporins and aquaglyceroporins belong to the ancient superfamily of Major Intrinsic Proteins (MIPs) and facilitate passive transport of water and small solutes across membranes of various organisms [ 1 , 2 ]. (biomedcentral.com)

Thus, this study defines the location of key residues in TM-A with respect to the lipid bilayer, describes the conformation of TM-A in a biomembrane mimic, presents a peptide-bilayer model useful in the consideration of local protein folding in the microsomal desaturases, and presents a model of arginine and tyrosine control of transmembrane protein stability and insertion. (shengsci.com)

Recently, engineered peptides, selected for their structural similarity to native palmitoylated proteins, have been synthesized and used to study palmitoylation using high performance liquid chromatography (HPLC)[28C30]. (epf2013.org)
BLOSUM matrix Starts by clustering proteins by similarity Avoids problems with small probabilities by using averages over clusters Numbering works opposite BLOSUM-62. (progsound.de)
Annotation transfer for function and structure within the sequence homology concept essentially requires protein sequence similarity for the secondary structural blocks forming the fold of a protein. (beds.ac.uk)
The sequence homology concept [ 1 - 3 ] is collectively founded upon the inductive reasoning that a homologous protein group (as an antecedent) shares a high level of sequence similarity (as a consequent) [ 4 - 8 ]. (beds.ac.uk)

Full names of ligands, sites, non-standard residues. (powerandbulk.com)
Some cytotoxic drugs tend to be inactive or less active when conjugated to large antibodies or protein receptor ligands. (justia.com)

However, Pauling never ceased advocating his valence bond theory, which continued to be used, for example, to explain the planarity of the peptide bond in proteins. (ucpress.edu)
An Evaluation of Peptide-Bond Isosteres Amit Choudhary[c] and Ronald T. Raines*[a, b] Peptide-bond isosteres can enable a deep interrogation of the structure and function of a peptide or protein by amplifying or attenuating particular chemical properties. (pdffox.com)
This vulnerability, along with a desire to modulate the structure and function of proteins, has motivated chemical biologists to develop surrogates of the peptide bond. (pdffox.com)
This delocalization is primarily responsible for the rotational barrier between the cis and trans isomers of the peptide bond, which can limit the rate of protein folding. (pdffox.com)

This variant showed substitution of methionine residues due to misincorporation of O -methyl- l -homoserine, also called methoxine. (biomedcentral.com)
Interpretation of PAM matrices PAM-1 one substitution per 100 residues (a PAM unit of time) Multiply them together to get PAM -100, etc. (progsound.de)
Viruses belonging to the largest cluster acquired an amino acid substitution in the receptor binding domain of the Spike protein (position 486), evolved faster and spread longer and more widely. (bvsalud.org)

A number of membrane proteins act via binding at the water/lipid bilayer interface. (shengsci.com)
The strong expression of ATP-gated P2X3 receptors by a subpopulation of sensory neurons indicates the important role of these membrane proteins in nociceptive signaling in health and disease, especially when the latter is accompanied by chronic pain syndromes. (gozmusic.org)

As reported earlier, the helical arrangement of the protein component of the vesicles facilitates image averaging and enables us to determine how cholesterol and phospho-lipid molecules are distributed in the surrounding matrix, using headgroup size as a means to discriminate between the two kinds of lipid. (iucr.org)
*Phospholipids are a class of lipids whose molecules have a hydrophilic (having affinity for water) head containing a phosphate group and two hydrophobic (water repelling) tails derived from fatty acids, joined by an alcohol residue. (futurelearn.com)
Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
Cooperative Motions of Protein and Hydration Water Molecules: Molecular Dynamics Study of Scytalone Dehydratase. (futatsugi.net)
Recent oxygen-17 magnetic relaxation dispersion (MRD) experiments have shown that the vast majority of water molecules in the protein hydration layer suffer a mere twofold dynamic retardation compared with bulk water. (lu.se)

Understanding the molecular basis of the C9orf72-SMCR8 protein-protein interaction is thus important to comprehend their function. (bvsalud.org)
The ankyrin repeat is one of the most common protein-protein interaction motifs in nature. (embl.de)
The interaction of POPDC proteins was investigated by co-precipitation, proximity ligation, bioluminescence resonance energy transfer and bimolecular fluorescence complementation. (imperial.ac.uk)
Site-directed mutagenesis was utilised to map the domains involved in protein-protein interaction. (imperial.ac.uk)
Nanoscale Hydrophobic Interaction and Nanobubble Nucleation. (futatsugi.net)
and (ii) the perturbation progress within the field of protein hydration has been of the structure and dynamics of bulk water caused by the interaction with the solute. (lu.se)

Transformation efficiencies of Ras mutants at residue 61 range over three orders of magnitude, but the in vitro GTPase activity decreases 10-fold for all mutants. (ncsu.edu)
2010). Lipid raft association and cholesterol sensitivity of P2X1-4 receptors for ATP: chimeras and point mutants identify intracellular amino-terminal residues involved in lipid regulation of P2X1 receptors. (gozmusic.org)

Site-directed mutagenesis of these residues confirms their importance in ARF-GAP activity. (embl.de)
Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H 2 their cofactor is trapped in an intermediate state indicative of precluded proton-transfer. (nature.com)

Structure-based sequence alignments were generated and the residues in the helix-helix interfaces were analyzed. (biomedcentral.com)
For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001. (lbl.gov)

The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. (biomedcentral.com)
Fluorescently-labeled tripeptide substrates representing the palmitoylation motif of ras proteins were used to characterize the activity of palmitoylation enzymes, known as acyltransferases, extracted from numerous cancer cell lines. (epf2013.org)

Formins are multidomain proteins defined by a conserved FH2 (formin homology 2) domain with actin nucleation activity preceded by a proline-rich FH1 (formin homology 1) domain. (biomedcentral.com)

Here, a systematic approach to investigate the effect of an active-site-residue mutation on a model enzyme, human carbonic anhydrase II (CA II), is described. (iucr.org)

Biologically active proteins and peptides, as those found in venoms, may have a potential therapeutic use for the correction of hemostatic disorders and cellular adhesion among other applications [ 8 ]. (biomedcentral.com)
Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. (biomedcentral.com)

VRACs are heteromeric assemblies of LRRC8A-E proteins with unknown stoichiometries. (elifesciences.org)

Cannabinoid receptor interacting protein 1a (CRIP1a) modulates CB1 cannabinoid receptor G-protein coupling in part by altering the selectivity for Gαi subtype activation, but the molecular basis for this function of CRIP1a is not known. (bvsalud.org)
In particular, the relationship between these dynamic nucleoprotein complexes and the molecular architecture of the ParB protein is unclear and is the subject of the work presented here. (elifesciences.org)
The major isoform of the human growth hormone is a protein of 191 amino acids and a molecular weight of 22,124 daltons. (powerandbulk.com)
Molecular dynamics study on class A $B&B (B-lactamase: hydrogen bond network among the functional groups of penicillin G and side chains of the conserved residues in the active site. (futatsugi.net)
Ab initio study of the role of lysine 16 for the molecular switching mechanism of Ras protein p21. (futatsugi.net)
The importance of the role of lysine 16 and GAP on the molecular switching mechanism of Ras protein p21. (futatsugi.net)
The proteins that make up the molecular machinery of life tends to fragment the research field into method-oriented have been perfected by several billion years of evolution subspecialties that rarely confront each other. (lu.se)
One contribution of 16 to a Discussion Meeting Issue `The molecular interact with a protein can, with little ambiguity, be classi- basis of life: is life possible without water? (lu.se)

Three different protein prenyltransferases (farnesyltransferase and geranylgeranyltransferases I and II) catalyze the attachment of prenyl lipid anchors 15 or 20 carbons long to the carboxyl termini of a variety of eukaryotic proteins. (biomedcentral.com)
Here, cryo-EM is used to examine the protein-lipid architecture of this membrane in tubular vesicles obtained from the (muscle-derived) electric organ of the Torpedo ray. (iucr.org)
Furthermore, its protein-lipid composition, organization and size are subject to modification by physiological events, making it an important mediator of neuronal plasticity. (iucr.org)
Here, we analyse by cryo-EM the architecture of this membrane in vesicles isolated from the (muscle-derived) electric organ of the Torpedo ray, with view to defining the protein-lipid interplay required to achieve an optimal neurotransmitter response. (iucr.org)
This indicates partial motional averaging and supports a model with the Val and Leu residues embedded inside the lipid bilayer. (shengsci.com)
The major intrinsic proteins (MIPs) facilitate the transport of water and neutral solutes across the lipid bilayers. (biomedcentral.com)

The ß-barrel has a gap between strands ß8 and ß10, which deviates from ß-sandwich fatty acid-binding proteins that carry endocannabinoid compounds and the Rho-guanine nucleotide dissociation inhibitor predicted by computational threading algorithms. (bvsalud.org)

Variants in MYBPC3, the gene encoding cardiac myosin-binding protein C (cMyBP-C), are the leading cause of HCM. (bvsalud.org)
Automatic comparisons of data from expressed sequence tags (ESTs) with genes (for example using the program Acembly, for which the results are available from the NCBI AceView server [ 2 ]) shows that all the human protein prenyltransferase genes have multiple alternative splice variants. (biomedcentral.com)

1999. Determination of organochlorine and organophosphate pesticide residues in fruits, vegetables and sediments. (cdc.gov)

PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular signaling domains including a pleckstrin homology domain, an SH3 domain, ankyrin repeats and an ARF-GAP domain. (embl.de)
c ) The 5.8 Å density map and superimposed 2.7 Å structure of the Torpedo receptor (PDB entry 6uwz ) obtained from detergent-solubilized protein complexed with α -bungarotoxin. (iucr.org)
The attachment of SARS-CoV-2 to a host cell is initiated by the spike protein trimer (S), which decorates the outer surface of the virus, binding to its cognate receptor angiotensin-converting enzyme 2 (ACE2), with higher affinity than SARS-CoV. (cmedlabsfoundation.com)
The FH1 domain is also a binding site for diverse SH3-domain containing proteins like Src-like non-receptor tyrosine kinases, WISH (WASP-interacting SH3 protein) and IRSp53 (insulin receptor substrate) in mammals, and Hof1p in yeast [ 6 ]. (biomedcentral.com)

Plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), Nod26-like intrinsic protein (NIPs) and small and basic intrinsic proteins (SIPs) are subfamilies of plant MIPs. (biomedcentral.com)

Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. (cdc.gov)
All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. (cdc.gov)

The tube-like shape of this density was consistent with a fatty acid, with size and shape similar to LA bound to other proteins. (cmedlabsfoundation.com)

The structural homology search program DALI identified CRIP1a as homologous to a family of lipidated-protein carriers that includes phosphodiesterase 6 delta subunit and Unc119. (bvsalud.org)

Hallmarks of FFA-binding pockets in proteins are an extended "greasy" tube lined by hydrophobic amino acids which accommodates the hydrocarbon tail, and a hydrophilic, often positively charged anchor for the acidic headgroup of the FFA. (cmedlabsfoundation.com)

Beta-propeller protein-associated neurodegeneration (BPAN) is associated with mutations in the autophagy gene WDR45. (bvsalud.org)
The protein and mRNA expression levels of WDR45 gene were decreased in the patient-derived fibroblasts. (bvsalud.org)
Gene structures and chromosomal locations of human protein prenyltransferase subunit genes. (biomedcentral.com)
the tri6 gene, encoding a zinc finger protein involved in the regulation of trichothecene biosynthesis, from Fusarium sporotrichioides (Proctor et al. (justia.com)
More specifically, the present invention concerns altering tolerance in plants to various environmental stresses and to increasing seed yield by modifying plant haemoglobin gene expression and/or by modifying plant haemoglobin protein levels. (allindianpatents.com)

The genomic organization of the human genes that encode protein prenyltransferases is shown in Figure 1 . (biomedcentral.com)
The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. (imperial.ac.uk)

The genes encoding CaaX protein prenyltransferases are considerably longer than those encoding non-CaaX subunits, as a result of longer introns. (biomedcentral.com)
(b) genes encoding non-CaaX protein prenyltransferases are much shorter. (biomedcentral.com)
The chromosomal locations and number of exons from protein prenyltransferase genes in the major eukaryotic model organisms are shown in Table 2 . (biomedcentral.com)
Mutations leading to loss of envelope (E) protein glycosylation together with mutations in the nonstructural (NS) protein genes may be associated with attenuation of these viruses ( 6 ). (cdc.gov)

Communication-deficient cells (the SKHep1 cell line) were stably transfected with a plasmid containing cDNA which encodes the major gap junction protein of rat liver, connexin32. (shengsci.com)

Folding Dynamics of 10-Residue Beta-Hairpin Peptide Chignolin. (futatsugi.net)
Protein hydration--the perturbation of water structure and dynamics by the protein surface--has been a particularly rich source of controversy and confusion. (lu.se)

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)

We introduce multiple interface string alignment (MISA), a visualization tool to display coherently various sequence and structure based statistics at protein-protein interfaces (SSE elements, buried surface area, ΔASA , B factor values, etc). (bvsalud.org)

The NS4B protein may play an important role in virulence phenotype determination ( 6 , 8 - 10 ), predicted to be involved in viral replication and evasion of host innate immune defenses ( 8 ). (cdc.gov)
The translated polyprotein consists of three structural [capsid (C), membrane (M) and envelope (E)] and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5). (spandidos-publications.com)

Comparison with these proteins suggests that CRIP1a may carry two possible types of cargo: either (i) like phosphodiesterase 6 delta subunit, cargo with a farnesyl moiety that enters from the top of the ß-barrel to occupy the hydrophobic interior or (ii) like Unc119, cargo with a palmitoyl or a myristoyl moiety that enters from the side where the missing ß-strand creates an opening to the hydrophobic pocket. (bvsalud.org)

Proteins or peptide substrates are incubated with tritiated palmitoyl coenzyme A ([3H]palmitoyl-CoA) with or without enzymes. (epf2013.org)

A C-terminal ataxin-2 disordered region promotes Huntingtin protein aggregation and neurodegeneration in Drosophila models of Huntington's disease. (tcd.ie)

Transcripts from peanut, soybean, sesame, and mite allergens contained a higher density of gaps than those of nonallergens. (jci.org)
Allergen transcripts translate into proteins with a cationic carboxy terminus depleted in hydrophobic residues. (jci.org)

Adv. Protein Chem. (crossref.org)

Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta. (embl.de)
The crystal structure of the PAPbeta ARF-GAP domain and the C-terminal ankyrin repeats has been determined at 2.1 A resolution. (embl.de)
Four ankyrin repeats are also present, the first two of which form an extensive interface with the ARF-GAP domain. (embl.de)

The native dimer reveals several asymmetric features including the arrangement of its two monomeric components, the structural integrity of protein-U, the overall organization of LH1, and rigidities of the proteins and pigments. (nature.com)
sphaeroides IL106 and the monomeric LH1-RC from a protein-U-deleted mutant strain (strain ΔU) of Rba . (nature.com)

Transcription infidelity (TI) is a mechanism that increases RNA and protein diversity. (jci.org)

The ΔU LH1-RC monomer was half-moon-shaped and contained 11 α- and 10 β-polypeptides, indicating a critical role for protein-U in controlling the number of αβ-subunits required for dimer assembly and stabilization. (nature.com)

It identifies binding sites and allows for a more thorough examination of protein plasticity and hydration than could be achieved by a single structure. (ncsu.edu)
Our aim here is to critically examine central concepts in the description of protein hydration, and to assess the experimental basis for the current view of protein hydration, with the focus on dynamic aspects. (lu.se)
The high mobility of hydration water ensures that all thermally activated processes at the protein-water interface, such as binding, recognition and catalysis, can proceed at high rates. (lu.se)
It is also inconsistent with the common view of hydration effects on protein hydrodynamics. (lu.se)
During this evolutionary process, the experimental basis of the current (multiple) views on proteins have adapted to and exploited the unique physi- protein hydration, with an emphasis on the dynamic cal properties of liquid water (Eisenberg & Kauzmann aspects. (lu.se)

We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)

An analysis of local frustration profiles indicated that (1) the structural domains in C9orf72 are minimally-frustrated and relatively conserved, (2) high frustration patches on the protein-protein interface (3) increased frustration in the C-terminal helices involved in the dimerization of C9orf72 structures. (bvsalud.org)

Instead of detecting the (13)C labeling patterns in the typically used amino acids from the total cellular protein, our method detects these (13)C patterns in amino acids from the reporter protein that has been expressed in only one species of the consortium. (gozmusic.org)

Anatomy12

Organisms10

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Phenomena and Processes82

Disciplines and Occupations6

Technology, Industry, Agriculture3

Information Science11

Health Care4

Cysteine7

Amino acid residues2

Sequences14

Interface residues1

Macromolecules3

Functionally diverse proteins2

Interactions9

Solvent5

Recombinant3

Peptides and proteins2

Superfamily4

Arginine1

Similarity4

Ligands2

Peptide bond4

Substitution3

Membrane proteins2

Molecules5

Interaction6

Mutants2

Mutagenesis2

Alignments2

Tripeptide2

Proline1

Mutation1

Therapeutic2

Heteromeric1

Molecular8

Lipid6

Nucleotide1

Variants2

19991

Receptor4

Intrinsic1

Strains2

Fatty1

Homology1

Hallmarks1

Gene5

Encode2

Genes4

Plasmid1

Dynamics2

BLAST1

Interfaces1

NS4B2

Subunit1

Substrates1

Neurodegeneration1

Transcripts2

Chem1

Repeats3

Monomeric2

Diversity1

Critical1

Hydration5

Receptors1

Structural1

Species1